MD12G1236400.v1.1

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014757
Physical Location & Seq
Reverse (-)
31038539 .. 31039612
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1236400.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGCGTGGCGGCCATTGCTTAGTTGGATCATGTCTTCTTCTTCCTCCTCTTCTTCGTCTTCTTATTCATCGTCAGAGTATGAATCTGACCATGACGATGATATTTGTCGTCATGACCCCGGCAACAACAATGTCCGGCAAGCACAAAAGGGTCACGATGTTGATGATCATGAAGACGATGACGATGAAAACGACGTTTCATATGAACCCCATGAGGCCGAGGACGAGGACGAAGTGGAGGAACCAAAACCCAGCTCCACCTCCGACTACAGCTTCACAATATACGAGCCTCGAGCAAAACGACGGCGTATTGACAATGCAAACGAGGCATTAACGTCCGTCCAAGAGGAAAAGAAGCGGCGGGGTAAGAGGGTTTGGAGTAAGGAGGACGAGCTCGAGCTGTTGCGTGGCTTTCTTGACTACTACAGCACCAGAAAAACCAGAATTACCAAAGCCACCGCTAAGCAAACGGCGTTGTTTTACGAAGAGATCAAACCGAGGTTAAAGCTCGAATGCGGCAAGACAAAAATGGTGGAAAAGCTGAGAAGACTAAAGAGGAAGTACCACAATGTCGTGAAGAAAATGAACGAAATCAACTCCTCCGGGAAAGAGTTCCGGTTTAAAAACGCACACGACAATGCGGTTTTCGAGCTTTCGCATAAGATTTGGAACGTCGAAAAAACCAGAAATAATTGTGTCGTAGAATCGCATAACAATAATGTCATGGTGAAGAGGGAGGAGGACAATGTGGCGGCGAGTAGTAGCGTTGCGGATCATGAGAGGCCAACGACGTCGTTTGATGAGAATAATAACGTTACTAATGTGGATGATAACATGAATAATGGTCATGATGTGAGAGGGTTGATAGTAGGGTTGGCTATGAATCCAATGCCTCTGAGTTTGAGAGGTAAAATGGGAAGTGGCGGCGGCAGCGGAGTCGGAGAAGTGGTGGAGGATGAGAAGTGGAGGCAGCAGCAGATTTTGGAGTTGAAGGCTTATGAAAAGAGGTTGGATTTGGTGCAGCATCAGGTTAAGGCAGCTTTGGAGGAATTGAGGTCCAAGGGAGGAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

358

Amino Acids

40.92

Weight (kDa)

5.74

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 123 - 224 2.8e-24 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 794
AclI AACGTT 1 cut(s) 813
AclWI GGATC 2 cut(s) 35, 780
AcoI YGGCCR 1 cut(s) 11
AcyI GRCGYC 1 cut(s) 791
AfaI GTAC 1 cut(s) 563
AgsI TTSAA 1 cut(s) 991
AluBI AGCT 9 cut(s) 255, 273, 394, 400, 508, 541, 652, 1040, 1070
AluI AGCT 9 cut(s) 255, 273, 394, 400, 508, 541, 652, 1040, 1070
Alw21I GWGCWC 1 cut(s) 396
AlwI GGATC 2 cut(s) 35, 780
Ama87I CYCGRG 2 cut(s) 291, 395
AoxI GGCC 3 cut(s) 11, 216, 782
ApeKI GCWGC 5 cut(s) 930, 970, 973, 1021, 1037
Asp700I GAANNNNTTC 1 cut(s) 611
AspS9I GGNCC 1 cut(s) 1056
AsuC2I CCSGG 2 cut(s) 120, 604
AsuHPI GGTGA 1 cut(s) 739
AvaI CYCGRG 2 cut(s) 291, 395
AvaII GGWCC 1 cut(s) 1056
BanII GRGCYC 1 cut(s) 396
BbsI GAAGAC 4 cut(s) 27, 51, 180, 553
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 5 cut(s) 942, 982, 985, 1033, 1049
BceAI ACGGC 2 cut(s) 320, 486
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BclI TGATCA 1 cut(s) 166
BcnI CCSGG 2 cut(s) 120, 604
BfmI CTRYAG 2 cut(s) 268, 424
BlpI GCTNAGC 1 cut(s) 462
Bme1390I CCNGG 2 cut(s) 120, 604
Bme18I GGWCC 1 cut(s) 1056
BmeT110I CYCGRG 2 cut(s) 291, 395
BmgT120I GGNCC 1 cut(s) 1056
BmiI GGNNCC 1 cut(s) 243
BmrFI CCNGG 2 cut(s) 120, 604
BmsI GCATC 1 cut(s) 1033
BpiI GAAGAC 4 cut(s) 27, 51, 180, 553
Bpu1102I GCTNAGC 1 cut(s) 462
BpuMI CCSGG 2 cut(s) 120, 604
BsaHI GRCGYC 1 cut(s) 791
BsaJI CCNNGG 4 cut(s) 118, 219, 497, 1059
BsaWI WCCGGW 1 cut(s) 615
BsaXI ACNNNNNCTCC 2 cut(s) 933, 963
Bse3DI GCAATG 1 cut(s) 14
BseDI CCNNGG 4 cut(s) 118, 219, 497, 1059
BseGI GGATG 2 cut(s) 832, 961
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 2 cut(s) 533, 887
BseRI GAGGAG 3 cut(s) 37, 589, 752
BseXI GCAGC 5 cut(s) 942, 982, 985, 1033, 1049
BseYI CCCAGC 1 cut(s) 251
BsgI GTGCAG 1 cut(s) 1040
BshFI GGCC 3 cut(s) 13, 218, 784
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 2 cut(s) 291, 395
BsiSI CCGG 4 cut(s) 120, 136, 603, 616
BsmI GAATGC 1 cut(s) 518
BsnI GGCC 3 cut(s) 13, 218, 784
BsoBI CYCGRG 2 cut(s) 291, 395
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 4 cut(s) 27, 166, 489, 772
Bsp1720I GCTNAGC 1 cut(s) 462
BspANI GGCC 3 cut(s) 13, 218, 784
BspCNI CTCAG 2 cut(s) 534, 888
BspHI TCATGA 4 cut(s) 112, 169, 775, 847
BspLI GGNNCC 1 cut(s) 243
BspPI GGATC 2 cut(s) 35, 780
BsrDI GCAATG 1 cut(s) 14
BssECI CCNNGG 4 cut(s) 118, 219, 497, 1059
BssMI GATC 4 cut(s) 27, 166, 489, 772
BssNI GRCGYC 1 cut(s) 791
BssT1I CCWWGG 1 cut(s) 1059
Bst6I CTCTTC 3 cut(s) 55, 480, 725
BstACI GRCGYC 1 cut(s) 791
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 5 cut(s) 20, 462, 542, 896, 1071
BstF5I GGATG 2 cut(s) 832, 961
BstKTI GATC 4 cut(s) 30, 169, 492, 775
BstMBI GATC 4 cut(s) 27, 166, 489, 772
BstMWI GCNNNNNNNGC 3 cut(s) 16, 326, 930
BstSCI CCNGG 2 cut(s) 118, 602
BstSFI CTRYAG 2 cut(s) 268, 424
BstV1I GCAGC 5 cut(s) 942, 982, 985, 1033, 1049
BstV2I GAAGAC 4 cut(s) 27, 51, 180, 553
BsuRI GGCC 3 cut(s) 13, 218, 784
BtsCI GGATG 2 cut(s) 832, 961
Cac8I GCNNGC 1 cut(s) 141
CciI TCATGA 4 cut(s) 112, 169, 775, 847
Cfr13I GGNCC 1 cut(s) 1056
Csp6I GTAC 1 cut(s) 562
CspCI CAANNNNNGTGG 2 cut(s) 513, 548
CviAII CATG 9 cut(s) 31, 92, 113, 170, 212, 724, 776, 835, 848
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 5 cut(s) 20, 462, 542, 896, 1071
DpnI GATC 4 cut(s) 29, 168, 491, 774
DpnII GATC 4 cut(s) 27, 166, 489, 772
DraI TTTAAA 1 cut(s) 622
EaeI YGGCCR 1 cut(s) 11
Eam1104I CTCTTC 3 cut(s) 55, 480, 725
EarI CTCTTC 3 cut(s) 55, 480, 725
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 1059
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 1056
Eco53kI GAGCTC 1 cut(s) 394
Eco88I CYCGRG 2 cut(s) 291, 395
EcoICRI GAGCTC 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 1059
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 1059
FaeI CATG 9 cut(s) 34, 95, 116, 173, 215, 727, 779, 838, 851
FatI CATG 9 cut(s) 30, 91, 112, 169, 211, 723, 775, 834, 847
FauI CCCGC 1 cut(s) 354
FauNDI CATATG 1 cut(s) 202
FbaI TGATCA 1 cut(s) 166
FokI GGATG 2 cut(s) 839, 968
FriOI GRGCYC 1 cut(s) 396
GsaI CCCAGC 1 cut(s) 255
HaeIII GGCC 3 cut(s) 13, 218, 784
HapII CCGG 4 cut(s) 120, 136, 603, 616
Hin1I GRCGYC 1 cut(s) 791
Hin1II CATG 9 cut(s) 34, 95, 116, 173, 215, 727, 779, 838, 851
HinfI GANTC 4 cut(s) 83, 704, 883, 936
HpaII CCGG 4 cut(s) 120, 136, 603, 616
HphI GGTGA 1 cut(s) 739
Hpy188I TCNGA 5 cut(s) 76, 88, 265, 897, 941
Hpy188III TCNNGA 7 cut(s) 113, 155, 170, 416, 574, 776, 848
Hpy99I CGWCG 5 cut(s) 197, 306, 677, 793, 796
HpyAV CCTTC 1 cut(s) 985
HpyCH4IV ACGT 5 cut(s) 195, 335, 672, 791, 813
HpyCH4V TGCA 2 cut(s) 320, 1021
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 326, 930
HpyF3I CTNAG 5 cut(s) 20, 462, 542, 896, 1071
HpySE526I ACGT 5 cut(s) 195, 335, 672, 791, 813
Hsp92I GRCGYC 1 cut(s) 791
Hsp92II CATG 9 cut(s) 34, 95, 116, 173, 215, 727, 779, 838, 851
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 4 cut(s) 27, 166, 489, 772
LmnI GCTCC 2 cut(s) 260, 1067
LpnPI CCDG 9 cut(s) 133, 149, 265, 445, 454, 616, 629, 697, 1013
Lsp1109I GCAGC 5 cut(s) 942, 982, 985, 1033, 1049
LweI GCATC 1 cut(s) 1033
MaeII ACGT 5 cut(s) 195, 335, 672, 791, 813
MaeIII GTNAC 2 cut(s) 152, 814
MalI GATC 4 cut(s) 29, 168, 491, 774
MboI GATC 4 cut(s) 27, 166, 489, 772
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 3 cut(s) 444, 691, 1049
MlyI GAGTC 1 cut(s) 945
MmeI TCCRAC 4 cut(s) 5, 288, 919, 990
MroXI GAANNNNTTC 1 cut(s) 611
MseI TTAA 4 cut(s) 332, 503, 621, 1032
MslI CAYNNNNRTG 2 cut(s) 96, 636
MspA1I CMGCKG 1 cut(s) 933
MspI CCGG 4 cut(s) 120, 136, 603, 616
MspR9I CCNGG 2 cut(s) 120, 604
Mva1269I GAATGC 1 cut(s) 518
MwoI GCNNNNNNNGC 3 cut(s) 16, 326, 930
NciI CCSGG 2 cut(s) 120, 604
NdeI CATATG 1 cut(s) 202
NdeII GATC 4 cut(s) 27, 166, 489, 772
NlaIII CATG 9 cut(s) 34, 95, 116, 173, 215, 727, 779, 838, 851
NlaIV GGNNCC 1 cut(s) 243
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 152
PaeR7I CTCGAG 2 cut(s) 291, 395
PagI TCATGA 4 cut(s) 112, 169, 775, 847
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 298
PctI GAATGC 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 3 cut(s) 83, 704, 883
PfoI TCCNGGA 1 cut(s) 602
PleI GAGTC 1 cut(s) 944
PpsI GAGTC 1 cut(s) 944
Psp124BI GAGCTC 1 cut(s) 396
Psp1406I AACGTT 1 cut(s) 813
PspFI CCCAGC 1 cut(s) 251
PspN4I GGNNCC 1 cut(s) 243
PspPI GGNCC 1 cut(s) 1056
PspXI VCTCGAGB 2 cut(s) 291, 395
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 2 cut(s) 96, 636
SacI GAGCTC 1 cut(s) 396
SaqAI TTAA 4 cut(s) 332, 503, 621, 1032
Sau3AI GATC 4 cut(s) 27, 166, 489, 772
Sau96I GGNCC 1 cut(s) 1056
SchI GAGTC 1 cut(s) 945
ScrFI CCNGG 2 cut(s) 120, 604
SduI GDGCHC 1 cut(s) 396
SfaNI GCATC 1 cut(s) 1033
SfcI CTRYAG 2 cut(s) 268, 424
Sfr274I CTCGAG 2 cut(s) 291, 395
SinI GGWCC 1 cut(s) 1056
SlaI CTCGAG 2 cut(s) 291, 395
SmiMI CAYNNNNRTG 2 cut(s) 96, 636
SmlI CTYRAG 2 cut(s) 291, 395
SmoI CTYRAG 2 cut(s) 291, 395
Sse9I AATT 3 cut(s) 444, 691, 1049
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 118, 602
StyI CCWWGG 1 cut(s) 1059
TaiI ACGT 5 cut(s) 198, 338, 675, 794, 816
TaqI TCGA 5 cut(s) 292, 396, 510, 648, 675
TasI AATT 3 cut(s) 444, 691, 1049
TauI GCSGC 6 cut(s) 13, 361, 519, 755, 927, 930
TfiI GAWTC 3 cut(s) 83, 704, 883
Tru1I TTAA 4 cut(s) 332, 503, 621, 1032
Tru9I TTAA 4 cut(s) 332, 503, 621, 1032
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 5 cut(s) 930, 970, 973, 1021, 1037
Tsp45I GTSAC 1 cut(s) 152
TspGWI ACGGA 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 1056
XcmI CCANNNNNNNNNTGG 1 cut(s) 21
XhoI CTCGAG 2 cut(s) 291, 395
XmnI GAANNNNTTC 1 cut(s) 611
ZraI GACGTC 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.