RchiOBHm_Chr3g0452201

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0014757
Physical Location & Seq
Forward (+)
2939282 .. 2940437
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41941

Sequence Viewer

Length: 993 bp
ATGCAATTTTCTCAGACGCAGACCAAGCCATACTCAAAAGGAGAATATCAATCTCTGGTTTGGTCTGGGTCTTTTCAACTAACCAGAAAAATGCCGTGGCCATTGCTAAAATGGATCATGTATACTTCTTCGTCGTCTTCGTCTTCCTCTGAAGATTTCAGTGACGCCGAAGATGAAGGGACAAGTAAGAAGGGCAATGATCATCGTCGTGATCCCAACAAACAAGGAGAACAAAATCACGATGACGACTCCAATGAAGATGACTTTTCCTACAAACCCCAGGAAGACGAGACCTCCAACTCCTCCGACTATACTTTCACAATCAACGAACCGCGCAGAAAAAGACGTCGCATTGACAACAAGGAAGACTCAACAATTGTGAGTTTTAAACGCCGTAAGAGGATTTGGAGCAAGGAGGACGAGCTAGAGCTCTTGAAGAATTTCCTTGACTACAGAACCAGACCGAGGACGGCTAACGAAACCACGTCGTTTTACGACGAGGTTAACCCCAAGTTCAAGGACCAATTCAGCAAGCGAAAGCTGGCCGAGAAGATTGCAAGGTTGAAGAAGAAGCACCGCAACGTAGTAAACAAAATGGGGAACAATAATGGGCAAGAGTTTCGCTTCAGAAACCCACACGACAGAGCCATTTTCGATATTTCACATAGGATTTGGGCAGAATCCCCTCTTTACAATGTTCAGGAGAAGAACGTTGTGGTGGATTCACATGATGTGGGGAGTAGCAAAGCGTCGTTCGATGAGAAGCATAACAATGGGGATGATGAGGGTAACACTGACGTGCGAGAAATGATAATCGGGTTGGCGATGAATCCGATACCGTTGAGTTTAAGTAACTATGGAGGCCGTGGAGGAGAAGTGGTGGATGAGAAGTGGAAGGAGCAGCAGATACTGGAGTTGGAGGCGTACTCGAAGAGGTTGGAGTTGGTTCAACATCAGGTTAAGGCAGCTTTGGATGACTTGCGTTCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

330

Amino Acids

38.58

Weight (kDa)

6.74

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 133 - 225 4.7e-24 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 349
AccI GTMKAC 1 cut(s) 122
AccII CGCG 1 cut(s) 334
AciI CCGC 2 cut(s) 332, 577
AclI AACGTT 1 cut(s) 711
AclWI GGATC 2 cut(s) 122, 206
AcoI YGGCCR 2 cut(s) 98, 543
AcsI RAATTY 1 cut(s) 439
AcuI CTGAAG 2 cut(s) 171, 610
AcyI GRCGYC 2 cut(s) 165, 346
AfaI GTAC 1 cut(s) 926
AfiI CCNNNNNNNGG 1 cut(s) 465
AgsI TTSAA 5 cut(s) 77, 436, 517, 565, 950
AjiI CACGTC 2 cut(s) 486, 799
AjnI CCWGG 1 cut(s) 279
AleI CACNNNNGTG 1 cut(s) 797
AluBI AGCT 4 cut(s) 424, 430, 541, 968
AluI AGCT 4 cut(s) 424, 430, 541, 968
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 2 cut(s) 122, 206
AlwNI CAGNNNCTG 1 cut(s) 910
AoxI GGCC 3 cut(s) 98, 543, 862
ApeKI GCWGC 2 cut(s) 901, 965
ApoI RAATTY 1 cut(s) 439
Asp700I GAANNNNTTC 1 cut(s) 440
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 1 cut(s) 520
AvaII GGWCC 1 cut(s) 520
BaeI ACNNNNGTAYC 2 cut(s) 899, 932
BalI TGGCCA 1 cut(s) 100
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 4 cut(s) 129, 135, 291, 372
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 913, 977
BceAI ACGGC 4 cut(s) 79, 378, 486, 849
BcgI CGANNNNNNTGC 4 cut(s) 536, 570, 602, 636
BciT130I CCWGG 1 cut(s) 281
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 1 cut(s) 451
BisI GCNGC 2 cut(s) 902, 966
BlsI GCNGC 2 cut(s) 903, 967
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 520
BmgBI CACGTC 2 cut(s) 486, 799
BmgT120I GGNCC 1 cut(s) 520
BmrFI CCNGG 1 cut(s) 281
BpiI GAAGAC 4 cut(s) 129, 135, 291, 372
BplI GAGNNNNNCTC 2 cut(s) 911, 943
BpmI CTGGAG 1 cut(s) 932
BsaHI GRCGYC 2 cut(s) 165, 346
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 4 cut(s) 95, 279, 464, 865
BsaXI ACNNNNNCTCC 6 cut(s) 278, 308, 861, 864, 891, 894
Bsc4I CCNNNNNNNGG 1 cut(s) 465
Bse1I ACTGG 1 cut(s) 915
Bse3DI GCAATG 2 cut(s) 101, 202
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 4 cut(s) 95, 279, 464, 865
BseGI GGATG 3 cut(s) 784, 889, 979
BseLI CCNNNNNNNGG 1 cut(s) 465
BseMI GCAATG 2 cut(s) 101, 202
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 915
BseRI GAGGAG 2 cut(s) 292, 885
BseXI GCAGC 2 cut(s) 913, 977
Bsh1236I CGCG 1 cut(s) 334
BshFI GGCC 3 cut(s) 100, 545, 864
BsiHKAI GWGCWC 1 cut(s) 432
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 1 cut(s) 465
BsmAI GTCTC 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 3 cut(s) 100, 545, 864
Bso31I GGTCTC 1 cut(s) 284
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 3 cut(s) 114, 199, 211
BspACI CCGC 2 cut(s) 332, 577
BspANI GGCC 3 cut(s) 100, 545, 864
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 1 cut(s) 334
BspPI GGATC 2 cut(s) 122, 206
BspTNI GGTCTC 1 cut(s) 284
BsrDI GCAATG 2 cut(s) 101, 202
BsrI ACTGG 1 cut(s) 915
BssECI CCNNGG 4 cut(s) 95, 279, 464, 865
BssMI GATC 3 cut(s) 114, 199, 211
BssNAI GTATAC 1 cut(s) 123
BssNI GRCGYC 2 cut(s) 165, 346
Bst1107I GTATAC 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 840
Bst6I CTCTTC 1 cut(s) 926
BstACI GRCGYC 2 cut(s) 165, 346
BstC8I GCNNGC 2 cut(s) 533, 543
BstDEI CTNAG 1 cut(s) 12
BstDSI CCRYGG 2 cut(s) 95, 865
BstF5I GGATG 3 cut(s) 784, 889, 979
BstFNI CGCG 1 cut(s) 334
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 3 cut(s) 117, 202, 214
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 3 cut(s) 114, 199, 211
BstMWI GCNNNNNNNGC 1 cut(s) 25
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 451
BstUI CGCG 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 913, 977
BstV2I GAAGAC 4 cut(s) 129, 135, 291, 372
BstZ17I GTATAC 1 cut(s) 123
BsuRI GGCC 3 cut(s) 100, 545, 864
BtgI CCRYGG 2 cut(s) 95, 865
BtgZI GCGATG 1 cut(s) 839
BtrI CACGTC 2 cut(s) 486, 799
BtsCI GGATG 3 cut(s) 784, 889, 979
BtsIMutI CAGTG 2 cut(s) 166, 792
Cac8I GCNNGC 2 cut(s) 533, 543
CaiI CAGNNNCTG 1 cut(s) 910
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 520
CseI GACGC 3 cut(s) 25, 173, 738
Csp6I GTAC 1 cut(s) 925
CviAII CATG 2 cut(s) 118, 728
CviQI GTAC 1 cut(s) 925
DdeI CTNAG 1 cut(s) 12
DpnI GATC 3 cut(s) 116, 201, 213
DpnII GATC 3 cut(s) 114, 199, 211
DraI TTTAAA 1 cut(s) 388
EaeI YGGCCR 2 cut(s) 98, 543
Eam1104I CTCTTC 1 cut(s) 926
EarI CTCTTC 1 cut(s) 926
Ecl136II GAGCTC 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 520
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 2 cut(s) 171, 610
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 279
EcoT38I GRGCYC 1 cut(s) 432
FaeI CATG 2 cut(s) 121, 731
FaiI YATR 8 cut(s) 31, 119, 123, 312, 666, 729, 768, 858
FaqI GGGAC 1 cut(s) 193
FatI CATG 2 cut(s) 117, 727
FbaI TGATCA 1 cut(s) 199
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 2 cut(s) 902, 966
FokI GGATG 3 cut(s) 791, 896, 986
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 2 cut(s) 902, 966
FspBI CTAG 1 cut(s) 425
GlaI GCGC 1 cut(s) 335
GluI GCNGC 2 cut(s) 902, 966
GsuI CTGGAG 1 cut(s) 932
HaeIII GGCC 3 cut(s) 100, 545, 864
HgaI GACGC 3 cut(s) 25, 173, 738
HhaI GCGC 1 cut(s) 336
Hin1I GRCGYC 2 cut(s) 165, 346
Hin1II CATG 2 cut(s) 121, 731
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 5 cut(s) 248, 368, 680, 722, 829
HpaI GTTAAC 1 cut(s) 505
Hpy166II GTNNAC 3 cut(s) 123, 505, 589
Hpy188I TCNGA 5 cut(s) 15, 151, 307, 629, 834
Hpy188III TCNNGA 4 cut(s) 209, 239, 433, 701
Hpy8I GTNNAC 3 cut(s) 123, 505, 589
Hpy99I CGWCG 6 cut(s) 136, 210, 351, 490, 500, 754
HpyAV CCTTC 3 cut(s) 170, 184, 889
HpyCH4III ACNGT 1 cut(s) 840
HpyCH4IV ACGT 5 cut(s) 346, 485, 582, 711, 798
HpyCH4V TGCA 2 cut(s) 4, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 25
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 5 cut(s) 346, 485, 582, 711, 798
Hsp92I GRCGYC 2 cut(s) 165, 346
Hsp92II CATG 2 cut(s) 121, 731
HspAI GCGC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 505
Kzo9I GATC 3 cut(s) 114, 199, 211
LmnI GCTCC 2 cut(s) 408, 898
Lsp1109I GCAGC 2 cut(s) 913, 977
MaeI CTAG 1 cut(s) 425
MaeII ACGT 5 cut(s) 346, 485, 582, 711, 798
MaeIII GTNAC 3 cut(s) 161, 788, 851
MalI GATC 3 cut(s) 116, 201, 213
MboI GATC 3 cut(s) 114, 199, 211
MfeI CAATTG 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 432
MlsI TGGCCA 1 cut(s) 100
MluCI AATT 4 cut(s) 5, 375, 439, 524
MluNI TGGCCA 1 cut(s) 100
MlyI GAGTC 2 cut(s) 242, 362
MmeI TCCRAC 4 cut(s) 321, 330, 897, 918
Mox20I TGGCCA 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 440
MscI TGGCCA 1 cut(s) 100
MseI TTAA 4 cut(s) 387, 504, 848, 960
MslI CAYNNNNRTG 3 cut(s) 207, 771, 797
Msp20I TGGCCA 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 281
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 1 cut(s) 334
MwoI GCNNNNNNNGC 1 cut(s) 25
NdeII GATC 3 cut(s) 114, 199, 211
NlaIII CATG 2 cut(s) 121, 731
NmeAIII GCCGAG 1 cut(s) 571
NmuCI GTSAC 1 cut(s) 161
OliI CACNNNNGTG 1 cut(s) 797
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 3 cut(s) 680, 722, 829
PkrI GCNGC 2 cut(s) 903, 967
PleI GAGTC 2 cut(s) 242, 362
PpsI GAGTC 2 cut(s) 242, 362
Psp124BI GAGCTC 1 cut(s) 432
Psp1406I AACGTT 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 1 cut(s) 520
PstNI CAGNNNCTG 1 cut(s) 910
RsaI GTAC 1 cut(s) 926
RsaNI GTAC 1 cut(s) 925
RseI CAYNNNNRTG 3 cut(s) 207, 771, 797
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 4 cut(s) 387, 504, 848, 960
SatI GCNGC 2 cut(s) 902, 966
Sau3AI GATC 3 cut(s) 114, 199, 211
Sau96I GGNCC 1 cut(s) 520
SchI GAGTC 2 cut(s) 242, 362
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 432
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 1 cut(s) 520
SmiMI CAYNNNNRTG 3 cut(s) 207, 771, 797
Sse9I AATT 4 cut(s) 5, 375, 439, 524
SsiI CCGC 2 cut(s) 332, 577
SspMI CTAG 1 cut(s) 425
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 840
TaiI ACGT 5 cut(s) 349, 488, 585, 714, 801
TaqI TCGA 3 cut(s) 654, 756, 929
TaqII GACCGA 1 cut(s) 478
TasI AATT 4 cut(s) 5, 375, 439, 524
TfiI GAWTC 3 cut(s) 680, 722, 829
Tru1I TTAA 4 cut(s) 387, 504, 848, 960
Tru9I TTAA 4 cut(s) 387, 504, 848, 960
TscAI CASTG 2 cut(s) 166, 799
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 2 cut(s) 901, 965
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 3 cut(s) 189, 270, 842
TspRI CASTG 2 cut(s) 166, 799
VpaK11BI GGWCC 1 cut(s) 520
XapI RAATTY 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
XmiI GTMKAC 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 440
XspI CTAG 1 cut(s) 425
ZraI GACGTC 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.