MD04G1226100.v1.1

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0017185
Physical Location & Seq
Reverse (-)
30568550 .. 30575871
7322 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1226100.v1.1.491

Sequence Viewer

Length: 585 bp
ATGAAGGACAATAACTTACACCGACGGAAGATGTCATCATCGTCGCCGTTATTGTCCCTTTCGTCCTCCGAGGAGGAGCTCCAACACATGCCGCTGGCACCGCCCAAGACGTTGAGCCGGAAGCGCTTGAGCAAGCAGTTGTCCATGTGCGAGACGCCTAGGGACATCGCGTGGGAGAGGCGGCAGCGCCAGATTCTCAGCCAGGAGAGGAGGATGAATGGGATCAGAGACTCCGATGACATGCATCTGACCGACGAGGATTTGCATGAGCTCAAAGGGTGCATTGAGCTAGGGTTTGGGTTCAATGAGGAGGAGGGTCAGAGGTTGAGCGCTACCTTGCCCGCTCTTGACCTTTACTTCGCCGTCAACCGCCAGTTTTCACCGAGCCCCGCGTCCACGCCGAATAGCGCTAGCGCCCGCCATTCTACGTCGTCAATGGGTGGACATTACTCGTCGTTTGGAAGCCCTAGGAGCGATACAGATGCATGGAAGATATGCAGTCCAGGTGATAATCCTGAACAGGTGAAGACCAAGTTGAGGCATTGGGCACAAGCTGTGGCTTGCTCAGTGATCCAATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

195

Amino Acids

21.79

Weight (kDa)

7.75

Isoelectric Point (pI)

84.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 83 - 138 3.5e-25 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08790
fragaria_vesca FvH4_6g02671
malus_domestica MD04G1226100.v1.1 MD12G1243200.v1.1
prunus_persica Prupe.6G344900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0450641
rosa_laevigata RLG00000025715
rosa_multiflora Rmu_sc0000078.1_g000040
rosa_rugosa Rorug02G0629200
rosa_samantha Rh3AG029900 Rh3BG030300 Rh3CG029200 Rh3DG030000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 97
AccBSI CCGCTC 1 cut(s) 344
AccII CGCG 2 cut(s) 170, 392
AciI CCGC 7 cut(s) 92, 101, 181, 342, 370, 390, 418
AclWI GGATC 2 cut(s) 230, 565
AcyI GRCGYC 1 cut(s) 155
AfeI AGCGCT 3 cut(s) 125, 331, 409
AfiI CCNNNNNNNGG 1 cut(s) 537
AgsI TTSAA 1 cut(s) 304
AjnI CCWGG 2 cut(s) 201, 502
AluBI AGCT 4 cut(s) 79, 271, 289, 554
AluI AGCT 4 cut(s) 79, 271, 289, 554
Alw21I GWGCWC 2 cut(s) 81, 273
Alw26I GTCTC 2 cut(s) 146, 222
AlwI GGATC 2 cut(s) 230, 565
Aor51HI AGCGCT 3 cut(s) 125, 331, 409
ApeKI GCWGC 1 cut(s) 184
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AspA2I CCTAGG 2 cut(s) 158, 467
AspLEI GCGC 5 cut(s) 126, 189, 332, 410, 416
AsuHPI GGTGA 3 cut(s) 372, 518, 535
AsuNHI GCTAGC 1 cut(s) 410
AvrII CCTAGG 2 cut(s) 158, 467
BaeGI GKGCMC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 3 cut(s) 81, 273, 389
BbsI GAAGAC 1 cut(s) 533
Bbv12I GWGCWC 2 cut(s) 81, 273
BbvI GCAGC 1 cut(s) 196
BceAI ACGGC 2 cut(s) 31, 347
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 2 cut(s) 203, 504
BcoDI GTCTC 2 cut(s) 146, 222
BfaI CTAG 4 cut(s) 159, 290, 411, 468
BfoI RGCGCY 5 cut(s) 127, 190, 333, 411, 417
BisI GCNGC 3 cut(s) 92, 182, 185
BlnI CCTAGG 2 cut(s) 158, 467
BlsI GCNGC 3 cut(s) 93, 183, 186
Bme1390I CCNGG 2 cut(s) 203, 504
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 2 cut(s) 203, 504
BmsI GCATC 2 cut(s) 253, 472
BmtI GCTAGC 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 148
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 69, 158, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 537
Bse1I ACTGG 1 cut(s) 373
BseBI CCWGG 2 cut(s) 203, 504
BseDI CCNNGG 3 cut(s) 69, 158, 467
BseGI GGATG 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 537
BseMII CTCAG 2 cut(s) 211, 579
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 5 cut(s) 86, 89, 223, 323, 326
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 1 cut(s) 196
Bsh1236I CGCG 2 cut(s) 170, 392
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 2 cut(s) 81, 273
BsiSI CCGG 1 cut(s) 118
BslFI GGGAC 2 cut(s) 40, 176
BslI CCNNNNNNNGG 1 cut(s) 537
BsmAI GTCTC 2 cut(s) 146, 222
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 40, 176
Bsp1286I GDGCHC 4 cut(s) 81, 273, 389, 550
Bsp143I GATC 2 cut(s) 222, 570
BspACI CCGC 7 cut(s) 92, 101, 181, 342, 370, 390, 418
BspCNI CTCAG 2 cut(s) 210, 578
BspFNI CGCG 2 cut(s) 170, 392
BspLI GGNNCC 1 cut(s) 99
BspOI GCTAGC 1 cut(s) 414
BspPI GGATC 2 cut(s) 230, 565
BspT107I GGYRCC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 344
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 3 cut(s) 69, 158, 467
BssMI GATC 2 cut(s) 222, 570
BssNI GRCGYC 1 cut(s) 155
BssT1I CCWWGG 2 cut(s) 158, 467
Bst2UI CCWGG 2 cut(s) 203, 504
BstACI GRCGYC 1 cut(s) 155
BstC8I GCNNGC 6 cut(s) 96, 134, 342, 412, 418, 562
BstDEI CTNAG 2 cut(s) 197, 565
BstF5I GGATG 1 cut(s) 219
BstFNI CGCG 2 cut(s) 170, 392
BstH2I RGCGCY 5 cut(s) 127, 190, 333, 411, 417
BstHHI GCGC 5 cut(s) 126, 189, 332, 410, 416
BstKTI GATC 2 cut(s) 225, 573
BstMAI GTCTC 2 cut(s) 146, 222
BstMBI GATC 2 cut(s) 222, 570
BstMWI GCNNNNNNNGC 3 cut(s) 100, 123, 471
BstNI CCWGG 2 cut(s) 203, 504
BstNSI RCATGY 2 cut(s) 91, 244
BstSCI CCNGG 2 cut(s) 201, 502
BstSLI GKGCMC 1 cut(s) 550
BstUI CGCG 2 cut(s) 170, 392
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 533
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 573
Cac8I GCNNGC 6 cut(s) 96, 134, 342, 412, 418, 562
CfoI GCGC 5 cut(s) 126, 189, 332, 410, 416
CseI GACGC 2 cut(s) 163, 381
CviAII CATG 5 cut(s) 88, 145, 241, 266, 486
CviJI RGCY 9 cut(s) 79, 117, 201, 271, 289, 387, 465, 554, 560
CviKI_1 RGCY 9 cut(s) 79, 117, 201, 271, 289, 387, 465, 554, 560
DdeI CTNAG 2 cut(s) 197, 565
DpnI GATC 2 cut(s) 224, 572
DpnII GATC 2 cut(s) 222, 570
Ecl136II GAGCTC 2 cut(s) 79, 271
Eco130I CCWWGG 2 cut(s) 158, 467
Eco24I GRGCYC 3 cut(s) 81, 273, 389
Eco47III AGCGCT 3 cut(s) 125, 331, 409
Eco53kI GAGCTC 2 cut(s) 79, 271
EcoICRI GAGCTC 2 cut(s) 79, 271
EcoRII CCWGG 2 cut(s) 201, 502
EcoT14I CCWWGG 2 cut(s) 158, 467
EcoT22I ATGCAT 2 cut(s) 246, 487
EcoT38I GRGCYC 3 cut(s) 81, 273, 389
ErhI CCWWGG 2 cut(s) 158, 467
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 5 cut(s) 91, 148, 244, 269, 489
FaiI YATR 6 cut(s) 89, 146, 242, 267, 487, 496
FaqI GGGAC 2 cut(s) 40, 176
FatI CATG 5 cut(s) 87, 144, 240, 265, 485
FauI CCCGC 3 cut(s) 349, 397, 425
Fnu4HI GCNGC 3 cut(s) 92, 182, 185
FokI GGATG 1 cut(s) 226
FriOI GRGCYC 3 cut(s) 81, 273, 389
Fsp4HI GCNGC 3 cut(s) 92, 182, 185
FspBI CTAG 4 cut(s) 159, 290, 411, 468
GlaI GCGC 5 cut(s) 125, 188, 331, 409, 415
GluI GCNGC 3 cut(s) 92, 182, 185
HaeII RGCGCY 5 cut(s) 127, 190, 333, 411, 417
HapII CCGG 1 cut(s) 118
HgaI GACGC 2 cut(s) 163, 381
HhaI GCGC 5 cut(s) 126, 189, 332, 410, 416
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 5 cut(s) 91, 148, 244, 269, 489
Hin6I GCGC 5 cut(s) 124, 187, 330, 408, 414
HinP1I GCGC 5 cut(s) 124, 187, 330, 408, 414
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 2 cut(s) 193, 230
HpaII CCGG 1 cut(s) 118
HphI GGTGA 3 cut(s) 372, 518, 535
Hpy166II GTNNAC 3 cut(s) 367, 396, 443
Hpy188I TCNGA 5 cut(s) 70, 227, 235, 249, 321
Hpy188III TCNNGA 3 cut(s) 347, 515, 582
Hpy8I GTNNAC 3 cut(s) 367, 396, 443
Hpy99I CGWCG 5 cut(s) 27, 46, 257, 433, 457
HpyCH4IV ACGT 2 cut(s) 110, 428
HpyCH4V TGCA 5 cut(s) 244, 265, 282, 485, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 123, 471
HpyF3I CTNAG 2 cut(s) 197, 565
HpySE526I ACGT 2 cut(s) 110, 428
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 5 cut(s) 91, 148, 244, 269, 489
HspAI GCGC 5 cut(s) 124, 187, 330, 408, 414
Kzo9I GATC 2 cut(s) 222, 570
LmnI GCTCC 3 cut(s) 76, 84, 471
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 2 cut(s) 253, 472
MaeI CTAG 4 cut(s) 159, 290, 411, 468
MaeII ACGT 2 cut(s) 110, 428
MalI GATC 2 cut(s) 224, 572
MbiI CCGCTC 1 cut(s) 344
MboI GATC 2 cut(s) 222, 570
MboII GAAGA 4 cut(s) 40, 502, 538, 570
MhlI GDGCHC 4 cut(s) 81, 273, 389, 550
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 106
Mph1103I ATGCAT 2 cut(s) 246, 487
MspA1I CMGCKG 1 cut(s) 94
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 2 cut(s) 203, 504
MvaI CCWGG 2 cut(s) 203, 504
MvnI CGCG 2 cut(s) 170, 392
MwoI GCNNNNNNNGC 3 cut(s) 100, 123, 471
NdeII GATC 2 cut(s) 222, 570
NheI GCTAGC 1 cut(s) 410
NlaIII CATG 5 cut(s) 91, 148, 244, 269, 489
NlaIV GGNNCC 1 cut(s) 99
NsiI ATGCAT 2 cut(s) 246, 487
NspI RCATGY 2 cut(s) 91, 244
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 3 cut(s) 93, 183, 186
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Psp124BI GAGCTC 2 cut(s) 81, 273
Psp6I CCWGG 2 cut(s) 201, 502
PspGI CCWGG 2 cut(s) 201, 502
PspN4I GGNNCC 1 cut(s) 99
SacI GAGCTC 2 cut(s) 81, 273
SatI GCNGC 3 cut(s) 92, 182, 185
Sau3AI GATC 2 cut(s) 222, 570
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 203, 504
SduI GDGCHC 4 cut(s) 81, 273, 389, 550
SfaNI GCATC 2 cut(s) 253, 472
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SsiI CCGC 7 cut(s) 92, 101, 181, 342, 370, 390, 418
SspMI CTAG 4 cut(s) 159, 290, 411, 468
SstI GAGCTC 2 cut(s) 81, 273
StyD4I CCNGG 2 cut(s) 201, 502
StyI CCWWGG 2 cut(s) 158, 467
TaiI ACGT 2 cut(s) 113, 431
TaqII GACCGA 1 cut(s) 266
TauI GCSGC 2 cut(s) 94, 184
TfiI GAWTC 1 cut(s) 193
TscAI CASTG 1 cut(s) 573
TseI GCWGC 1 cut(s) 184
TspDTI ATGAA 2 cut(s) 17, 230
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 1 cut(s) 573
XceI RCATGY 2 cut(s) 91, 244
XmaJI CCTAGG 2 cut(s) 158, 467
XspI CTAG 4 cut(s) 159, 290, 411, 468
Zsp2I ATGCAT 2 cut(s) 246, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.