Rh3AG029900

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0017185
Physical Location & Seq
Forward (+)
2005653 .. 2008010
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG029900.1

Sequence Viewer

Length: 666 bp
ATGGATATGAACGACGTCGAGCAAATACCCGCGCCGCAGCCGGGGAAGTCATCGTTGTACTCGGAGTCATCGCCGTGCCTGTCGCTTTCGTCCTCCTCCGAGGAGCTGCAGGACATGCCGCTGGCGCCGCCGAAGATGATGAGCCGGAAGAGGCTGTGCAAGCAGCTGTCCATGTGCGAGACGCCGCGTGACATTGCATGGGAGAAGCGGCGCCGGCAAATTCTGAAGCAGGAGAGGAAGAGAAAGAGGAACGGAAATGGTGATTCCCCCGGCGCCGCCGCCGAACCAGAGGGAGACGACATGAACCTGACCGACGAGGACTTGCATGAGCTGAAAGGGTGTATAGAGCTAGGGTTTGGGTTCAACGAGGAAGAGGGTCAACGGTTGTGCCCCACATTGCCGGCTCTTGACCTTTACTTTGCCGTTAACCGCCAGTTCTCGCCGAGTCCGATCTCCTCGCCGAATAGCAGCCGACATTCCACGTCGTCAATTGGTGGTGCGACGTCGTCATTTGGTGGACTTTCATCGTCGTTTGGAAGCCCTAGCCCTAGGAGCGACAGTGATGGATGGAAGATATGCAGCCCAGGTGATAATCCTCAACAGGTGAAGACCAAGTTGAGGCATTGGGCGCAAGCAGTTGCTTGTTCAGTTATGCAATCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

221

Amino Acids

24.24

Weight (kDa)

5.98

Isoelectric Point (pI)

90.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 103 - 159 2.5e-25 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08790
fragaria_vesca FvH4_6g02671
malus_domestica MD04G1226100.v1.1 MD12G1243200.v1.1
prunus_persica Prupe.6G344900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0450641
rosa_laevigata RLG00000025715
rosa_multiflora Rmu_sc0000078.1_g000040
rosa_rugosa Rorug02G0629200
rosa_samantha Rh3AG029900 Rh3BG030300 Rh3CG029200 Rh3DG030000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 18, 506
AccB1I GGYRCC 3 cut(s) 124, 210, 272
AccII CGCG 2 cut(s) 32, 187
AciI CCGC 9 cut(s) 30, 35, 119, 128, 185, 208, 276, 279, 430
AcsI RAATTY 1 cut(s) 219
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 2 cut(s) 41, 618
AgsI TTSAA 1 cut(s) 364
AjiI CACGTC 1 cut(s) 483
AjnI CCWGG 1 cut(s) 583
AluBI AGCT 4 cut(s) 106, 166, 331, 349
AluI AGCT 4 cut(s) 106, 166, 331, 349
Alw26I GTCTC 2 cut(s) 173, 288
ApeKI GCWGC 5 cut(s) 37, 106, 163, 468, 579
ApoI RAATTY 1 cut(s) 219
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AspA2I CCTAGG 1 cut(s) 548
AspLEI GCGC 5 cut(s) 34, 127, 213, 275, 631
AsuC2I CCSGG 2 cut(s) 42, 270
AsuHPI GGTGA 3 cut(s) 272, 599, 616
AvrII CCTAGG 1 cut(s) 548
BaeGI GKGCMC 1 cut(s) 392
BanI GGYRCC 3 cut(s) 124, 210, 272
BbsI GAAGAC 1 cut(s) 614
BbvI GCAGC 5 cut(s) 49, 93, 175, 480, 591
BccI CCATC 2 cut(s) 557, 561
BceAI ACGGC 2 cut(s) 58, 407
BciT130I CCWGG 1 cut(s) 585
BcnI CCSGG 2 cut(s) 42, 270
BcoDI GTCTC 2 cut(s) 173, 288
BfaI CTAG 3 cut(s) 350, 543, 549
BfmI CTRYAG 1 cut(s) 107
BfoI RGCGCY 3 cut(s) 128, 214, 276
BlnI CCTAGG 1 cut(s) 548
Bme1390I CCNGG 3 cut(s) 42, 270, 585
BmgBI CACGTC 1 cut(s) 483
BmiI GGNNCC 3 cut(s) 126, 212, 274
BmrFI CCNGG 3 cut(s) 42, 270, 585
BpiI GAAGAC 1 cut(s) 614
BpuMI CCSGG 2 cut(s) 42, 270
BsaHI GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
BsaJI CCNNGG 5 cut(s) 41, 99, 268, 548, 583
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 618
Bse118I RCCGGY 2 cut(s) 213, 400
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 2 cut(s) 192, 395
BseBI CCWGG 1 cut(s) 585
BseDI CCNNGG 5 cut(s) 41, 99, 268, 548, 583
BseGI GGATG 1 cut(s) 572
BseLI CCNNNNNNNGG 2 cut(s) 41, 618
BseMI GCAATG 2 cut(s) 192, 395
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 3 cut(s) 85, 116, 445
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 5 cut(s) 49, 93, 175, 480, 591
Bsh1236I CGCG 2 cut(s) 32, 187
BshNI GGYRCC 3 cut(s) 124, 210, 272
BsiSI CCGG 5 cut(s) 41, 145, 214, 270, 401
BslI CCNNNNNNNGG 2 cut(s) 41, 618
BsmAI GTCTC 2 cut(s) 173, 288
BsmBI CGTCTC 2 cut(s) 173, 288
Bsp1286I GDGCHC 1 cut(s) 392
Bsp143I GATC 1 cut(s) 450
BspACI CCGC 9 cut(s) 30, 35, 119, 128, 185, 208, 276, 279, 430
BspFNI CGCG 2 cut(s) 32, 187
BspLI GGNNCC 3 cut(s) 126, 212, 274
BspMAI CTGCAG 1 cut(s) 111
BspT107I GGYRCC 3 cut(s) 124, 210, 272
BsrDI GCAATG 2 cut(s) 192, 395
BsrFI RCCGGY 2 cut(s) 213, 400
BsrI ACTGG 1 cut(s) 433
BssAI RCCGGY 2 cut(s) 213, 400
BssECI CCNNGG 5 cut(s) 41, 99, 268, 548, 583
BssMI GATC 1 cut(s) 450
BssNI GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
BssT1I CCWWGG 1 cut(s) 548
Bst2UI CCWGG 1 cut(s) 585
Bst4CI ACNGT 2 cut(s) 384, 560
Bst6I CTCTTC 3 cut(s) 143, 233, 366
BstACI GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
BstAPI GCANNNNNTGC 1 cut(s) 115
BstC8I GCNNGC 5 cut(s) 123, 161, 215, 402, 633
BstF5I GGATG 1 cut(s) 572
BstFNI CGCG 2 cut(s) 32, 187
BstH2I RGCGCY 3 cut(s) 128, 214, 276
BstHHI GCGC 5 cut(s) 34, 127, 213, 275, 631
BstKTI GATC 1 cut(s) 453
BstMAI GTCTC 2 cut(s) 173, 288
BstMBI GATC 1 cut(s) 450
BstMWI GCNNNNNNNGC 7 cut(s) 115, 124, 127, 160, 214, 552, 628
BstNI CCWGG 1 cut(s) 585
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 3 cut(s) 40, 268, 583
BstSFI CTRYAG 1 cut(s) 107
BstSLI GKGCMC 1 cut(s) 392
BstUI CGCG 2 cut(s) 32, 187
BstV1I GCAGC 5 cut(s) 49, 93, 175, 480, 591
BstV2I GAAGAC 1 cut(s) 614
BtgZI GCGATG 1 cut(s) 54
BtrI CACGTC 1 cut(s) 483
BtsCI GGATG 1 cut(s) 572
BtsIMutI CAGTG 1 cut(s) 565
Cac8I GCNNGC 5 cut(s) 123, 161, 215, 402, 633
CfoI GCGC 5 cut(s) 34, 127, 213, 275, 631
Cfr10I RCCGGY 2 cut(s) 213, 400
CseI GACGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 58
CviAII CATG 5 cut(s) 115, 172, 198, 301, 326
CviQI GTAC 1 cut(s) 58
DinI GGCGCC 3 cut(s) 126, 212, 274
DpnI GATC 1 cut(s) 452
DpnII GATC 1 cut(s) 450
Eam1104I CTCTTC 3 cut(s) 143, 233, 366
EarI CTCTTC 3 cut(s) 143, 233, 366
Eco130I CCWWGG 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 583
EcoT14I CCWWGG 1 cut(s) 548
EgeI GGCGCC 3 cut(s) 126, 212, 274
EheI GGCGCC 3 cut(s) 126, 212, 274
ErhI CCWWGG 1 cut(s) 548
Esp3I CGTCTC 2 cut(s) 173, 288
FaeI CATG 5 cut(s) 118, 175, 201, 304, 329
FaiI YATR 9 cut(s) 8, 116, 173, 199, 302, 327, 344, 577, 653
FatI CATG 5 cut(s) 114, 171, 197, 300, 325
FauI CCCGC 1 cut(s) 37
FokI GGATG 1 cut(s) 579
FspBI CTAG 3 cut(s) 350, 543, 549
GlaI GCGC 5 cut(s) 33, 126, 212, 274, 630
HaeII RGCGCY 3 cut(s) 128, 214, 276
HapII CCGG 5 cut(s) 41, 145, 214, 270, 401
HgaI GACGC 1 cut(s) 190
HhaI GCGC 5 cut(s) 34, 127, 213, 275, 631
Hin1I GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
Hin1II CATG 5 cut(s) 118, 175, 201, 304, 329
Hin6I GCGC 5 cut(s) 32, 125, 211, 273, 629
HinP1I GCGC 5 cut(s) 32, 125, 211, 273, 629
HincII GTYRAC 2 cut(s) 380, 427
HindII GTYRAC 2 cut(s) 380, 427
HinfI GANTC 3 cut(s) 65, 263, 445
HpaI GTTAAC 1 cut(s) 427
HpaII CCGG 5 cut(s) 41, 145, 214, 270, 401
HphI GGTGA 3 cut(s) 272, 599, 616
Hpy166II GTNNAC 3 cut(s) 380, 427, 518
Hpy188I TCNGA 4 cut(s) 64, 100, 225, 450
Hpy188III TCNNGA 1 cut(s) 407
Hpy8I GTNNAC 3 cut(s) 380, 427, 518
Hpy99I CGWCG 7 cut(s) 17, 20, 317, 487, 505, 508, 532
HpyCH4III ACNGT 2 cut(s) 384, 560
HpyCH4IV ACGT 3 cut(s) 15, 482, 503
HpyCH4V TGCA 6 cut(s) 109, 159, 197, 325, 579, 655
HpyF10VI GCNNNNNNNGC 7 cut(s) 115, 124, 127, 160, 214, 552, 628
HpySE526I ACGT 3 cut(s) 15, 482, 503
Hsp92I GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
Hsp92II CATG 5 cut(s) 118, 175, 201, 304, 329
HspAI GCGC 5 cut(s) 32, 125, 211, 273, 629
KasI GGCGCC 3 cut(s) 124, 210, 272
KroI GCCGGC 2 cut(s) 213, 400
KroNI GCCGGC 2 cut(s) 215, 402
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 1 cut(s) 450
LmnI GCTCC 2 cut(s) 103, 552
Lsp1109I GCAGC 5 cut(s) 49, 93, 175, 480, 591
MaeI CTAG 3 cut(s) 350, 543, 549
MaeII ACGT 3 cut(s) 15, 482, 503
MaeIII GTNAC 1 cut(s) 188
MalI GATC 1 cut(s) 452
MboI GATC 1 cut(s) 450
MboII GAAGA 6 cut(s) 145, 160, 250, 383, 583, 619
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 392
MluCI AATT 2 cut(s) 219, 489
Mly113I GGCGCC 3 cut(s) 125, 211, 273
MlyI GAGTC 2 cut(s) 74, 454
MroNI GCCGGC 2 cut(s) 213, 400
MseI TTAA 2 cut(s) 426, 664
MslI CAYNNNNRTG 1 cut(s) 73
MspA1I CMGCKG 2 cut(s) 121, 166
MspI CCGG 5 cut(s) 41, 145, 214, 270, 401
MspR9I CCNGG 3 cut(s) 42, 270, 585
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 585
MvnI CGCG 2 cut(s) 32, 187
MwoI GCNNNNNNNGC 7 cut(s) 115, 124, 127, 160, 214, 552, 628
NaeI GCCGGC 2 cut(s) 215, 402
NarI GGCGCC 3 cut(s) 125, 211, 273
NciI CCSGG 2 cut(s) 42, 270
NdeII GATC 1 cut(s) 450
NgoMIV GCCGGC 2 cut(s) 213, 400
NlaIII CATG 5 cut(s) 118, 175, 201, 304, 329
NlaIV GGNNCC 3 cut(s) 126, 212, 274
NmeAIII GCCGAG 1 cut(s) 468
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 1 cut(s) 118
PcsI WCGNNNNNNNCGW 1 cut(s) 446
PdiI GCCGGC 2 cut(s) 215, 402
PfeI GAWTC 1 cut(s) 263
PflFI GACNNNGTC 1 cut(s) 505
PleI GAGTC 2 cut(s) 73, 453
PluTI GGCGCC 3 cut(s) 128, 214, 276
PpsI GAGTC 2 cut(s) 73, 453
Psp6I CCWGG 1 cut(s) 583
PspGI CCWGG 1 cut(s) 583
PspN4I GGNNCC 3 cut(s) 126, 212, 274
PstI CTGCAG 1 cut(s) 111
PsyI GACNNNGTC 1 cut(s) 505
PvuII CAGCTG 1 cut(s) 166
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 73
SaqAI TTAA 2 cut(s) 426, 664
Sau3AI GATC 1 cut(s) 450
SchI GAGTC 2 cut(s) 74, 454
ScrFI CCNGG 3 cut(s) 42, 270, 585
SduI GDGCHC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 107
SfoI GGCGCC 3 cut(s) 126, 212, 274
SmiMI CAYNNNNRTG 1 cut(s) 73
Sse9I AATT 2 cut(s) 219, 489
SsiI CCGC 9 cut(s) 30, 35, 119, 128, 185, 208, 276, 279, 430
SspDI GGCGCC 3 cut(s) 124, 210, 272
SspMI CTAG 3 cut(s) 350, 543, 549
StyD4I CCNGG 3 cut(s) 40, 268, 583
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 2 cut(s) 384, 560
TaiI ACGT 3 cut(s) 18, 485, 506
TaqI TCGA 1 cut(s) 18
TaqII GACCGA 1 cut(s) 326
TasI AATT 2 cut(s) 219, 489
TatI WGTACW 1 cut(s) 57
TauI GCSGC 7 cut(s) 37, 121, 130, 187, 211, 278, 281
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 2 cut(s) 426, 664
Tru9I TTAA 2 cut(s) 426, 664
TscAI CASTG 1 cut(s) 565
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 5 cut(s) 37, 106, 163, 468, 579
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 3 cut(s) 23, 317, 513
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 565
Tth111I GACNNNGTC 1 cut(s) 505
XapI RAATTY 1 cut(s) 219
XceI RCATGY 1 cut(s) 118
XmaJI CCTAGG 1 cut(s) 548
XspI CTAG 3 cut(s) 350, 543, 549
ZraI GACGTC 2 cut(s) 16, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.