MD12G1243200.v1.1

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017185
Physical Location & Seq
Reverse (-)
31395024 .. 31396972
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1243200.v1.1.491

Sequence Viewer

Length: 585 bp
ATGAAGGACGAGAACTTACACCGAAGGAAGATGTCGTCATCGTCGCCATCACTATCCCTTTCATCCTCCTCCGAGGAGCTGCAGCACATGCCGCTGGCCCCACCCAAGATGTTGAACCGGAAGCGCCTGAGCAAGCAGCTGTCCATGTGCGAGACGCCTAGGGACATCGCCTGGGAGAGGCGGCGGCGCCAGATTCTCAGCCAGGAGAGGAGGATGCACGGGATCAGAGACTCCGAAGACATGCATCTGACGGACGAGGACTTGCATGAACTCAAAGGGTGCATTGAGCTAGGGTTTGGGTTCAATGAGGAGGAAGGTCAGAGGTTGAGCGCTACCTTGCCCGCTCTTGACCTTTACTTCGCGGTCAACCGCCAGTATTCCCTGAGCCCCGTGTCCACACCGAATAGCGCTAGCTCCCGCCATTCTACTTCGTCAATGGGTGGACATTCGTTGTCGTTTGGAAGCCCTACGAGCGATACCGATGCATGGAAGATATGCAGTCCAGGTGATAATCCTGAACAGGTGAAGACCAAATTGAGGCATTGGGCACAAGCTGTGGCTTGTTCAGTGATCCAGTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

195

Amino Acids

21.83

Weight (kDa)

7.76

Isoelectric Point (pI)

89.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 83 - 139 1.4e-25 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08790
fragaria_vesca FvH4_6g02671
malus_domestica MD04G1226100.v1.1 MD12G1243200.v1.1
prunus_persica Prupe.6G344900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0450641
rosa_laevigata RLG00000025715
rosa_multiflora Rmu_sc0000078.1_g000040
rosa_rugosa Rorug02G0629200
rosa_samantha Rh3AG029900 Rh3BG030300 Rh3CG029200 Rh3DG030000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 186
AccBSI CCGCTC 1 cut(s) 344
AccII CGCG 1 cut(s) 362
AciI CCGC 7 cut(s) 92, 181, 184, 342, 362, 370, 418
AclWI GGATC 2 cut(s) 230, 565
AcyI GRCGYC 2 cut(s) 155, 187
AfeI AGCGCT 2 cut(s) 331, 409
AfiI CCNNNNNNNGG 3 cut(s) 177, 486, 537
AgsI TTSAA 2 cut(s) 115, 304
AjnI CCWGG 3 cut(s) 170, 201, 502
AluBI AGCT 5 cut(s) 79, 139, 289, 414, 554
AluI AGCT 5 cut(s) 79, 139, 289, 414, 554
Alw26I GTCTC 2 cut(s) 146, 222
AlwI GGATC 2 cut(s) 230, 565
Aor51HI AGCGCT 2 cut(s) 331, 409
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 3 cut(s) 79, 82, 136
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AspA2I CCTAGG 1 cut(s) 158
AspLEI GCGC 4 cut(s) 126, 189, 332, 410
AspS9I GGNCC 1 cut(s) 97
AsuHPI GGTGA 2 cut(s) 518, 535
AsuNHI GCTAGC 1 cut(s) 410
AvrII CCTAGG 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 389
BbsI GAAGAC 3 cut(s) 243, 533, 570
BbvI GCAGC 3 cut(s) 66, 94, 148
BccI CCATC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 3 cut(s) 172, 203, 504
BcoDI GTCTC 2 cut(s) 146, 222
BfaI CTAG 3 cut(s) 159, 290, 411
BfmI CTRYAG 1 cut(s) 80
BfoI RGCGCY 4 cut(s) 127, 190, 333, 411
BisI GCNGC 6 cut(s) 80, 83, 92, 137, 182, 185
BlnI CCTAGG 1 cut(s) 158
BlsI GCNGC 6 cut(s) 81, 84, 93, 138, 183, 186
Bme1390I CCNGG 3 cut(s) 172, 203, 504
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 2 cut(s) 99, 188
BmrFI CCNGG 3 cut(s) 172, 203, 504
BmsI GCATC 3 cut(s) 204, 253, 472
BmtI GCTAGC 1 cut(s) 414
BpiI GAAGAC 3 cut(s) 243, 533, 570
Bpu10I CCTNAGC 2 cut(s) 128, 383
BsaHI GRCGYC 2 cut(s) 155, 187
BsaJI CCNNGG 3 cut(s) 72, 158, 171
BsaWI WCCGGW 1 cut(s) 117
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 486, 537
Bse1I ACTGG 2 cut(s) 373, 574
BseBI CCWGG 3 cut(s) 172, 203, 504
BseDI CCNNGG 3 cut(s) 72, 158, 171
BseGI GGATG 2 cut(s) 62, 219
BseLI CCNNNNNNNGG 3 cut(s) 177, 486, 537
BseMII CTCAG 3 cut(s) 119, 211, 374
BseNI ACTGG 2 cut(s) 373, 574
BseRI GAGGAG 4 cut(s) 58, 89, 223, 323
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 3 cut(s) 66, 94, 148
Bsh1236I CGCG 1 cut(s) 362
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 118
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 3 cut(s) 177, 486, 537
BsmAI GTCTC 2 cut(s) 146, 222
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 389, 550
Bsp143I GATC 2 cut(s) 222, 570
BspACI CCGC 7 cut(s) 92, 181, 184, 342, 362, 370, 418
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 3 cut(s) 120, 210, 375
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 2 cut(s) 99, 188
BspMAI CTGCAG 1 cut(s) 84
BspOI GCTAGC 1 cut(s) 414
BspPI GGATC 2 cut(s) 230, 565
BspT107I GGYRCC 1 cut(s) 186
BsrBI CCGCTC 1 cut(s) 344
BsrI ACTGG 2 cut(s) 373, 574
BssECI CCNNGG 3 cut(s) 72, 158, 171
BssMI GATC 2 cut(s) 222, 570
BssNI GRCGYC 2 cut(s) 155, 187
BssT1I CCWWGG 1 cut(s) 158
Bst2UI CCWGG 3 cut(s) 172, 203, 504
BstACI GRCGYC 2 cut(s) 155, 187
BstAPI GCANNNNNTGC 1 cut(s) 88
BstC8I GCNNGC 4 cut(s) 96, 134, 342, 412
BstDEI CTNAG 3 cut(s) 128, 197, 383
BstENI CCTNNNNNAGG 1 cut(s) 175
BstF5I GGATG 2 cut(s) 62, 219
BstFNI CGCG 1 cut(s) 362
BstH2I RGCGCY 4 cut(s) 127, 190, 333, 411
BstHHI GCGC 4 cut(s) 126, 189, 332, 410
BstKTI GATC 2 cut(s) 225, 573
BstMAI GTCTC 2 cut(s) 146, 222
BstMBI GATC 2 cut(s) 222, 570
BstMWI GCNNNNNNNGC 3 cut(s) 88, 91, 471
BstNI CCWGG 3 cut(s) 172, 203, 504
BstNSI RCATGY 2 cut(s) 91, 244
BstSCI CCNGG 3 cut(s) 170, 201, 502
BstSFI CTRYAG 1 cut(s) 80
BstSLI GKGCMC 1 cut(s) 550
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 3 cut(s) 66, 94, 148
BstV2I GAAGAC 3 cut(s) 243, 533, 570
BsuRI GGCC 1 cut(s) 98
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 2 cut(s) 62, 219
BtsIMutI CAGTG 1 cut(s) 573
Cac8I GCNNGC 4 cut(s) 96, 134, 342, 412
CfoI GCGC 4 cut(s) 126, 189, 332, 410
Cfr13I GGNCC 1 cut(s) 97
CseI GACGC 1 cut(s) 163
CviAII CATG 5 cut(s) 88, 145, 241, 266, 486
DdeI CTNAG 3 cut(s) 128, 197, 383
DinI GGCGCC 1 cut(s) 188
DpnI GATC 2 cut(s) 224, 572
DpnII GATC 2 cut(s) 222, 570
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 389
Eco47III AGCGCT 2 cut(s) 331, 409
EcoNI CCTNNNNNAGG 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 170, 201, 502
EcoT14I CCWWGG 1 cut(s) 158
EcoT22I ATGCAT 2 cut(s) 246, 487
EcoT38I GRGCYC 1 cut(s) 389
EgeI GGCGCC 1 cut(s) 188
EheI GGCGCC 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 158
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 5 cut(s) 91, 148, 244, 269, 489
FaiI YATR 6 cut(s) 89, 146, 242, 267, 487, 496
FaqI GGGAC 1 cut(s) 176
FatI CATG 5 cut(s) 87, 144, 240, 265, 485
FauI CCCGC 2 cut(s) 349, 425
Fnu4HI GCNGC 6 cut(s) 80, 83, 92, 137, 182, 185
FokI GGATG 2 cut(s) 49, 226
FriOI GRGCYC 1 cut(s) 389
Fsp4HI GCNGC 6 cut(s) 80, 83, 92, 137, 182, 185
FspBI CTAG 3 cut(s) 159, 290, 411
GlaI GCGC 4 cut(s) 125, 188, 331, 409
GluI GCNGC 6 cut(s) 80, 83, 92, 137, 182, 185
HaeII RGCGCY 4 cut(s) 127, 190, 333, 411
HaeIII GGCC 1 cut(s) 98
HapII CCGG 1 cut(s) 118
HgaI GACGC 1 cut(s) 163
HhaI GCGC 4 cut(s) 126, 189, 332, 410
Hin1I GRCGYC 2 cut(s) 155, 187
Hin1II CATG 5 cut(s) 91, 148, 244, 269, 489
Hin6I GCGC 4 cut(s) 124, 187, 330, 408
HinP1I GCGC 4 cut(s) 124, 187, 330, 408
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 2 cut(s) 193, 230
HpaII CCGG 1 cut(s) 118
HphI GGTGA 2 cut(s) 518, 535
Hpy166II GTNNAC 3 cut(s) 367, 396, 443
Hpy188I TCNGA 5 cut(s) 73, 227, 235, 249, 321
Hpy188III TCNNGA 3 cut(s) 347, 515, 582
Hpy8I GTNNAC 3 cut(s) 367, 396, 443
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 18, 308
HpyCH4V TGCA 7 cut(s) 82, 217, 244, 265, 282, 485, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 91, 471
HpyF3I CTNAG 3 cut(s) 128, 197, 383
Hsp92I GRCGYC 2 cut(s) 155, 187
Hsp92II CATG 5 cut(s) 91, 148, 244, 269, 489
HspAI GCGC 4 cut(s) 124, 187, 330, 408
KasI GGCGCC 1 cut(s) 186
Kzo9I GATC 2 cut(s) 222, 570
LmnI GCTCC 2 cut(s) 76, 419
Lsp1109I GCAGC 3 cut(s) 66, 94, 148
LweI GCATC 3 cut(s) 204, 253, 472
MaeI CTAG 3 cut(s) 159, 290, 411
MalI GATC 2 cut(s) 224, 572
MbiI CCGCTC 1 cut(s) 344
MboI GATC 2 cut(s) 222, 570
MboII GAAGA 5 cut(s) 40, 248, 502, 538, 570
MhlI GDGCHC 2 cut(s) 389, 550
MluCI AATT 1 cut(s) 533
Mly113I GGCGCC 1 cut(s) 187
MlyI GAGTC 1 cut(s) 224
Mph1103I ATGCAT 2 cut(s) 246, 487
MspA1I CMGCKG 2 cut(s) 94, 139
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 3 cut(s) 172, 203, 504
MvaI CCWGG 3 cut(s) 172, 203, 504
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 3 cut(s) 88, 91, 471
NarI GGCGCC 1 cut(s) 187
NdeII GATC 2 cut(s) 222, 570
NheI GCTAGC 1 cut(s) 410
NlaIII CATG 5 cut(s) 91, 148, 244, 269, 489
NlaIV GGNNCC 2 cut(s) 99, 188
NsiI ATGCAT 2 cut(s) 246, 487
NspI RCATGY 2 cut(s) 91, 244
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 6 cut(s) 81, 84, 93, 138, 183, 186
PleI GAGTC 1 cut(s) 224
PluTI GGCGCC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 224
Psp6I CCWGG 3 cut(s) 170, 201, 502
PspGI CCWGG 3 cut(s) 170, 201, 502
PspN4I GGNNCC 2 cut(s) 99, 188
PspPI GGNCC 1 cut(s) 97
PstI CTGCAG 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 139
SatI GCNGC 6 cut(s) 80, 83, 92, 137, 182, 185
Sau3AI GATC 2 cut(s) 222, 570
Sau96I GGNCC 1 cut(s) 97
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 3 cut(s) 172, 203, 504
SduI GDGCHC 2 cut(s) 389, 550
SfaNI GCATC 3 cut(s) 204, 253, 472
SfcI CTRYAG 1 cut(s) 80
SfoI GGCGCC 1 cut(s) 188
Sse9I AATT 1 cut(s) 533
SsiI CCGC 7 cut(s) 92, 181, 184, 342, 362, 370, 418
SspDI GGCGCC 1 cut(s) 186
SspMI CTAG 3 cut(s) 159, 290, 411
StyD4I CCNGG 3 cut(s) 170, 201, 502
StyI CCWWGG 1 cut(s) 158
TasI AATT 1 cut(s) 533
TauI GCSGC 3 cut(s) 94, 184, 187
TfiI GAWTC 1 cut(s) 193
TscAI CASTG 1 cut(s) 573
TseI GCWGC 3 cut(s) 79, 82, 136
TspDTI ATGAA 3 cut(s) 17, 51, 282
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 1 cut(s) 573
XagI CCTNNNNNAGG 1 cut(s) 175
XceI RCATGY 2 cut(s) 91, 244
XmaJI CCTAGG 1 cut(s) 158
XspI CTAG 3 cut(s) 159, 290, 411
Zsp2I ATGCAT 2 cut(s) 246, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.