MD04G1228400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014542
Physical Location & Seq
Forward (+)
30892044 .. 30893370
1327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1228400.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGCATCTCCTACTGCTTGTACTGATCCTTCCATGCGAGCCGATTACCATGCACTGTTTTCAGCAGCACAAGATGCCTCTGAGCGTCTCAGTAAAAAGCAGAAGAATCAGTTAACTACGTGGACTATCAGGGCAGTGATGGCAAATGAGTTATTCACCAACGACAGCTTCCTGTTTTCAAAAGCAGCTGGACGATTAAAGGAAGCCATATCTAATCTGCCTGTTGCAACTGAGGATGATGACAGAGAGGAAGCGGCTGTGCTGGAAGATGATACAAATTTAGCAAATGAAGATGATCCGACAAAGCAGGACGTGACTTCAGCTGCACCAGCGGAAAATAATGATGAGGAGTCTGATCCGTTTGGGCTTGATGCATTTTTAACTCCTGCCTCTACGAAGAAAGATGATAAAACAAAGGGAAAAACAGATAACATGATCACAAGTGAGGAGGAAGAGACCAAGAGATTCCTCAGGTCAAAAAGAGAAGCCTTGGTTCTTTGTTTAGAGATAGCTGCTCGGTGTTATAAAACCCCATGGTGCCAAACCGTCATAGACATATTAGCCAAGCACGCTTTTGATGACATAGCAAGGTTCACATCGAAACAAAGGAGTGCCATTGAGAAACTTTGGGCTTCCATACGGGAACAGCATAATCATAGAAAACAAGGGAAATCGGTCACTGGGAAACTCGATGTCAATGATTTCGAGTGGCTGCAGCAGAAGTATGCTACTGAGAAGATTAGCATTCGGCATTCAGTTGGAAATAGTGGGGATCGTAAAACTGAAATGTGGCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

29.94

Weight (kDa)

5.31

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014542)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04560
fragaria_vesca FvH4_6g02310
malus_domestica MD04G1228300.v1.1 MD04G1228400.v1.1 MD12G1245500.v1.1
prunus_persica Prupe.6G348300_v2.0.a1
pyrus_communis pycom12g22500
rosa_chinensis RchiOBHm_Chr3g0450061
rosa_laevigata RLG00000025760
rosa_multiflora Rmu_sc0002646.1_g000024
rosa_roxburghii Rroxscaffold_6G00427840
rosa_rugosa Rorug02G0625200
rosa_samantha Rh3AG024300 Rh3BG024900 Rh3CG023800 Rh3DG024900
rosa_wichuraiana Rw3G001940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 537
AciI CCGC 2 cut(s) 254, 332
AclWI GGATC 4 cut(s) 20, 290, 350, 780
AcsI RAATTY 1 cut(s) 277
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 1 cut(s) 22
AgsI TTSAA 1 cut(s) 180
AjiI CACGTC 1 cut(s) 313
AjuI GAANNNNNNNTTGG 3 cut(s) 152, 184, 777
AluBI AGCT 4 cut(s) 168, 188, 323, 512
AluI AGCT 4 cut(s) 168, 188, 323, 512
Alw26I GTCTC 2 cut(s) 92, 449
AlwI GGATC 4 cut(s) 20, 290, 350, 780
ApeKI GCWGC 6 cut(s) 65, 185, 323, 512, 712, 715
ApoI RAATTY 1 cut(s) 277
AsuHPI GGTGA 1 cut(s) 148
AxyI CCTNAGG 1 cut(s) 470
BanI GGYRCC 1 cut(s) 537
BarI GAAGNNNNNNTAC 2 cut(s) 13, 45
BbvI GCAGC 6 cut(s) 77, 197, 310, 499, 699, 727
BccI CCATC 1 cut(s) 133
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BclI TGATCA 1 cut(s) 435
BcoDI GTCTC 2 cut(s) 92, 449
BfmI CTRYAG 1 cut(s) 713
BisI GCNGC 7 cut(s) 66, 186, 255, 324, 513, 713, 716
BlsI GCNGC 7 cut(s) 67, 187, 256, 325, 514, 714, 717
BmgBI CACGTC 1 cut(s) 313
BmiI GGNNCC 1 cut(s) 539
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 3 cut(s) 14, 64, 361
BmuI ACTGGG 1 cut(s) 690
BsaAI YACGTR 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 2 cut(s) 489, 533
Bse1I ACTGG 1 cut(s) 685
Bse21I CCTNAGG 1 cut(s) 470
BseDI CCNNGG 2 cut(s) 489, 533
BseGI GGATG 1 cut(s) 241
BseMII CTCAG 5 cut(s) 72, 103, 222, 484, 723
BseNI ACTGG 1 cut(s) 685
BseRI GAGGAG 2 cut(s) 362, 461
BseXI GCAGC 6 cut(s) 77, 197, 310, 499, 699, 727
BsgI GTGCAG 1 cut(s) 309
BshNI GGYRCC 1 cut(s) 537
BsmAI GTCTC 2 cut(s) 92, 449
BsmBI CGTCTC 1 cut(s) 92
BsmI GAATGC 2 cut(s) 744, 751
Bso31I GGTCTC 1 cut(s) 449
Bsp143I GATC 5 cut(s) 25, 295, 355, 435, 772
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 2 cut(s) 254, 332
BspCNI CTCAG 5 cut(s) 73, 102, 223, 483, 724
BspLI GGNNCC 1 cut(s) 539
BspMAI CTGCAG 1 cut(s) 717
BspPI GGATC 4 cut(s) 20, 290, 350, 780
BspT107I GGYRCC 1 cut(s) 537
BspTNI GGTCTC 1 cut(s) 449
BsrI ACTGG 1 cut(s) 685
BssECI CCNNGG 2 cut(s) 489, 533
BssMI GATC 5 cut(s) 25, 295, 355, 435, 772
BssT1I CCWWGG 2 cut(s) 489, 533
Bst4CI ACNGT 2 cut(s) 57, 547
Bst6I CTCTTC 1 cut(s) 447
BstAPI GCANNNNNTGC 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 39, 570
BstDEI CTNAG 5 cut(s) 81, 89, 231, 470, 732
BstDSI CCRYGG 1 cut(s) 533
BstF5I GGATG 1 cut(s) 241
BstKTI GATC 5 cut(s) 28, 298, 358, 438, 775
BstMAI GTCTC 2 cut(s) 92, 449
BstMBI GATC 5 cut(s) 25, 295, 355, 435, 772
BstMWI GCNNNNNNNGC 4 cut(s) 74, 140, 329, 569
BstSFI CTRYAG 1 cut(s) 713
BstV1I GCAGC 6 cut(s) 77, 197, 310, 499, 699, 727
Bsu36I CCTNAGG 1 cut(s) 470
BtgI CCRYGG 1 cut(s) 533
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 3 cut(s) 53, 141, 678
Cac8I GCNNGC 2 cut(s) 39, 570
CseI GACGC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 21
CviAII CATG 4 cut(s) 34, 50, 433, 534
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 5 cut(s) 81, 89, 231, 470, 732
DpnI GATC 5 cut(s) 27, 297, 357, 437, 774
DpnII GATC 5 cut(s) 25, 295, 355, 435, 772
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Eco130I CCWWGG 2 cut(s) 489, 533
Eco31I GGTCTC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 303
Eco81I CCTNAGG 1 cut(s) 470
EcoT14I CCWWGG 2 cut(s) 489, 533
EcoT22I ATGCAT 1 cut(s) 376
ErhI CCWWGG 2 cut(s) 489, 533
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 4 cut(s) 37, 53, 436, 537
FatI CATG 4 cut(s) 33, 49, 432, 533
FbaI TGATCA 1 cut(s) 435
Fnu4HI GCNGC 7 cut(s) 66, 186, 255, 324, 513, 713, 716
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 7 cut(s) 66, 186, 255, 324, 513, 713, 716
GluI GCNGC 7 cut(s) 66, 186, 255, 324, 513, 713, 716
HgaI GACGC 1 cut(s) 74
Hin1II CATG 4 cut(s) 37, 53, 436, 537
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 3 cut(s) 106, 350, 465
HpaI GTTAAC 1 cut(s) 114
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 3 cut(s) 114, 123, 594
Hpy188I TCNGA 3 cut(s) 82, 300, 355
Hpy8I GTNNAC 3 cut(s) 114, 123, 594
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 2 cut(s) 57, 547
HpyCH4IV ACGT 2 cut(s) 119, 312
HpyCH4V TGCA 5 cut(s) 53, 227, 326, 374, 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 140, 329, 569
HpyF3I CTNAG 5 cut(s) 81, 89, 231, 470, 732
HpySE526I ACGT 2 cut(s) 119, 312
Hsp92II CATG 4 cut(s) 37, 53, 436, 537
Ksp22I TGATCA 1 cut(s) 435
KspAI GTTAAC 1 cut(s) 114
Kzo9I GATC 5 cut(s) 25, 295, 355, 435, 772
Lsp1109I GCAGC 6 cut(s) 77, 197, 310, 499, 699, 727
LweI GCATC 3 cut(s) 14, 64, 361
MaeII ACGT 2 cut(s) 119, 312
MaeIII GTNAC 2 cut(s) 313, 676
MalI GATC 5 cut(s) 27, 297, 357, 437, 774
MboI GATC 5 cut(s) 25, 295, 355, 435, 772
MboII GAAGA 6 cut(s) 115, 278, 302, 409, 464, 748
MluCI AATT 1 cut(s) 277
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 323, 739
MnlI CCTC 8 cut(s) 88, 226, 241, 340, 400, 439, 442, 479
Mph1103I ATGCAT 1 cut(s) 376
MseI TTAA 3 cut(s) 113, 197, 380
MspA1I CMGCKG 3 cut(s) 188, 323, 332
Mva1269I GAATGC 2 cut(s) 744, 751
MwoI GCNNNNNNNGC 4 cut(s) 74, 140, 329, 569
NcoI CCATGG 1 cut(s) 533
NdeII GATC 5 cut(s) 25, 295, 355, 435, 772
NlaIII CATG 4 cut(s) 37, 53, 436, 537
NlaIV GGNNCC 1 cut(s) 539
NmuCI GTSAC 2 cut(s) 313, 676
NsiI ATGCAT 1 cut(s) 376
PctI GAATGC 2 cut(s) 744, 751
PfeI GAWTC 2 cut(s) 106, 465
PkrI GCNGC 7 cut(s) 67, 187, 256, 325, 514, 714, 717
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Ppu21I YACGTR 1 cut(s) 120
PsiI TTATAA 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 539
PstI CTGCAG 1 cut(s) 717
PvuII CAGCTG 2 cut(s) 188, 323
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
SaqAI TTAA 3 cut(s) 113, 197, 380
SatI GCNGC 7 cut(s) 66, 186, 255, 324, 513, 713, 716
Sau3AI GATC 5 cut(s) 25, 295, 355, 435, 772
SchI GAGTC 1 cut(s) 359
SetI ASST 8 cut(s) 122, 170, 190, 315, 325, 476, 514, 593
SfaNI GCATC 3 cut(s) 14, 64, 361
SfcI CTRYAG 1 cut(s) 713
Sse9I AATT 1 cut(s) 277
SsiI CCGC 2 cut(s) 254, 332
StyI CCWWGG 2 cut(s) 489, 533
TaaI ACNGT 2 cut(s) 57, 547
TaiI ACGT 2 cut(s) 122, 315
TaqI TCGA 3 cut(s) 599, 690, 705
TaqII GACCGA 1 cut(s) 664
TasI AATT 1 cut(s) 277
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 106, 465
Tru1I TTAA 3 cut(s) 113, 197, 380
Tru9I TTAA 3 cut(s) 113, 197, 380
TscAI CASTG 3 cut(s) 60, 141, 685
TseFI GTSAC 2 cut(s) 313, 676
TseI GCWGC 6 cut(s) 65, 185, 323, 512, 712, 715
Tsp45I GTSAC 2 cut(s) 313, 676
TspDTI ATGAA 1 cut(s) 303
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 3 cut(s) 60, 141, 685
XapI RAATTY 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.