pycom12g22500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014542
Physical Location & Seq
Forward (+)
23375449 .. 23377802
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g22500.5

Sequence Viewer

Length: 1332 bp
ATGGAGAGATCCAATCGGGTTGGAACTCCTCCCTTTTTGGACAAGCTCCGAACCAGTTCGATAGAGAGGGAAAGGGACATGGCTGACGACCTGTTTGAAGGCCTCCCTCCGCCGTCTGCAGACCCTCCTCCTCCTCCTTCTCCTGAAATTCAACTCCGGCAACCGCGACCGGAACCACCAAAACCACCAAAACCTCGAAAAACAGCTTCTTTTAGTTGCGACTCTTCTTCCCCTGCTCCTGCTCCTCCTCCACCTGCTGCCCCTAAACCAATTCTCAAATCCGCCCTCAAGCGGCCGAATCCCGCGGATTCGACCCCAGAAGAAACTGTGCCGGAAAAGAAAAAGTTGAGGTTCAAAACCACGACGGATGCCTCAGAGCAACAGGTGATAGAGGCGATGCAGAAGATAGCTTCGCATATAAAGACTCCTGCGAAGTTTAGTAAGGCTTCAAAGCTTGCCATCCAGCTTATTCAGGCAGGAAGTGTGAAAGCTGAGACTAGTGGTTACTTTTTTGCTATACTAGAAGCCGTAATGGAATCTCCTACTTCTTGTACGGATCCTTCCATGCGAGCTGATTACCATGCATTGTTTTCAGCAGCACAAGATGCCTCTGAGTGTCTCGATAAGAAGCAAAAGAATCAATTAACGACATGGACAATCAGGGCAGTGATGGCAAATGAATTATTCACCGACGACAGCTTCCTGTTTTCAAAAGCAGCTGGACGATTAAAGGAAGCCATTTCTAATCTGCCTGTTGCAACCGAGGACGATGACAGAGAGGAAGCAGCTGTGCTGGAAGATGGAACAAAGATGGCAGATGAAGATGACCAGATGAAGCAGGACGTGACTTCAGCTGCACCAGCGGAAAATAATGATGAGTTGTCTGATCCTTTTGGGCTTGATGCATTTTTAACTCCTGCCTCTACAAAGAAAGATGATAGAACAAAGGAAAAAAAAGATAACATGATCACGAAGGAGGAGCAAGAGACCAATAGATTCCTCAGGTCAAAAAGGGAAGCCTTGGTTCTTTGTTTAGAGATAGCTGCTAGGCGTTATAAAACCCCATGGTGCCAAACAGTCATAGACATACTAGCAAAGCACGCCTTTGATAACATAGCAAGGTTCACATTGAAACAAAGGACTGCCATTGAGAAACTTTGGGTTTCCATACGGGAACAGCATAATCGTCGAAAACAAGGGAAATCTGTTACTGGGAAACTTGATGTCAATGCTTTCGAGTGGCTGCAGCAAAAATATTCTACTGAGAAGATCAGCATTCGGCACTCTGTTAGTGGTAGCGGAGGTCGTAAAACTGAAACGTGGCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

49.38

Weight (kDa)

8.1

Isoelectric Point (pI)

59.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014542)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04560
fragaria_vesca FvH4_6g02310
malus_domestica MD04G1228300.v1.1 MD04G1228400.v1.1 MD12G1245500.v1.1
prunus_persica Prupe.6G348300_v2.0.a1
pyrus_communis pycom12g22500
rosa_chinensis RchiOBHm_Chr3g0450061
rosa_laevigata RLG00000025760
rosa_multiflora Rmu_sc0002646.1_g000024
rosa_roxburghii Rroxscaffold_6G00427840
rosa_rugosa Rorug02G0625200
rosa_samantha Rh3AG024300 Rh3BG024900 Rh3CG023800 Rh3DG024900
rosa_wichuraiana Rw3G001940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1056
AarI CACCTGC 1 cut(s) 262
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 1068
AccII CGCG 2 cut(s) 166, 305
AciI CCGC 8 cut(s) 110, 164, 282, 292, 303, 305, 863, 1299
AclWI GGATC 4 cut(s) 3, 551, 564, 881
AcoI YGGCCR 1 cut(s) 293
AcsI RAATTY 1 cut(s) 147
AcuI CTGAAG 1 cut(s) 834
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 1 cut(s) 291
AgsI TTSAA 6 cut(s) 98, 152, 355, 450, 711, 1132
AhlI ACTAGT 1 cut(s) 497
AjiI CACGTC 1 cut(s) 844
AjuI GAANNNNNNNTTGG 1 cut(s) 1308
Alw26I GTCTC 3 cut(s) 488, 623, 980
AlwI GGATC 4 cut(s) 3, 551, 564, 881
AoxI GGCC 2 cut(s) 100, 293
ApeKI GCWGC 8 cut(s) 257, 596, 716, 785, 854, 1043, 1243, 1246
ApoI RAATTY 1 cut(s) 147
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
Asp700I GAANNNNTTC 1 cut(s) 55
AsuHPI GGTGA 2 cut(s) 397, 679
AxyI CCTNAGG 1 cut(s) 1001
BamHI GGATCC 1 cut(s) 556
BanI GGYRCC 1 cut(s) 1068
BarI GAAGNNNNNNTAC 2 cut(s) 544, 576
BbvI GCAGC 8 cut(s) 244, 608, 728, 797, 841, 1030, 1230, 1258
BccI CCATC 4 cut(s) 467, 664, 794, 805
BceAI ACGGC 2 cut(s) 97, 512
BcgI CGANNNNNNTGC 2 cut(s) 1169, 1203
BclI TGATCA 1 cut(s) 966
BcoDI GTCTC 3 cut(s) 488, 623, 980
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 4 cut(s) 498, 521, 1047, 1091
BfmI CTRYAG 2 cut(s) 117, 1244
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 9 cut(s) 258, 293, 597, 717, 786, 855, 1044, 1244, 1247
BlsI GCNGC 9 cut(s) 259, 294, 598, 718, 787, 856, 1045, 1245, 1248
BmgBI CACGTC 1 cut(s) 844
BmiI GGNNCC 3 cut(s) 174, 558, 1070
BmrI ACTGGG 1 cut(s) 1221
BmsI GCATC 4 cut(s) 358, 387, 595, 892
BmuI ACTGGG 1 cut(s) 1221
BpuEI CTTGAG 1 cut(s) 272
BsaI GGTCTC 1 cut(s) 980
BsaJI CCNNGG 4 cut(s) 303, 762, 1020, 1064
BsaWI WCCGGW 1 cut(s) 169
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse1I ACTGG 2 cut(s) 54, 1216
Bse21I CCTNAGG 1 cut(s) 1001
BseDI CCNNGG 4 cut(s) 303, 762, 1020, 1064
BseGI GGATG 2 cut(s) 373, 459
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMII CTCAG 5 cut(s) 387, 483, 603, 1015, 1254
BseNI ACTGG 2 cut(s) 54, 1216
BseRI GAGGAG 7 cut(s) 18, 117, 120, 123, 234, 237, 992
BseX3I CGGCCG 1 cut(s) 293
BseXI GCAGC 8 cut(s) 244, 608, 728, 797, 841, 1030, 1230, 1258
BsgI GTGCAG 1 cut(s) 840
Bsh1236I CGCG 2 cut(s) 166, 305
Bsh1285I CGRYCG 2 cut(s) 170, 296
BshFI GGCC 2 cut(s) 102, 295
BshNI GGYRCC 1 cut(s) 1068
BsiEI CGRYCG 2 cut(s) 170, 296
BsiSI CCGG 3 cut(s) 157, 170, 332
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 1 cut(s) 291
BsmAI GTCTC 3 cut(s) 488, 623, 980
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 1275
BsnI GGCC 2 cut(s) 102, 295
Bso31I GGTCTC 1 cut(s) 980
Bsp143I GATC 5 cut(s) 8, 556, 886, 966, 1269
Bsp19I CCATGG 1 cut(s) 1064
BspACI CCGC 8 cut(s) 110, 164, 282, 292, 303, 305, 863, 1299
BspANI GGCC 2 cut(s) 102, 295
BspCNI CTCAG 5 cut(s) 386, 484, 604, 1014, 1255
BspFNI CGCG 2 cut(s) 166, 305
BspLI GGNNCC 3 cut(s) 174, 558, 1070
BspMAI CTGCAG 2 cut(s) 121, 1248
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 4 cut(s) 3, 551, 564, 881
BspT107I GGYRCC 1 cut(s) 1068
BspTNI GGTCTC 1 cut(s) 980
BsrI ACTGG 2 cut(s) 54, 1216
BssECI CCNNGG 4 cut(s) 303, 762, 1020, 1064
BssMI GATC 5 cut(s) 8, 556, 886, 966, 1269
BssT1I CCWWGG 2 cut(s) 1020, 1064
Bst4CI ACNGT 2 cut(s) 328, 1078
Bst6I CTCTTC 1 cut(s) 229
BstAPI GCANNNNNTGC 1 cut(s) 605
BstC8I GCNNGC 3 cut(s) 456, 570, 1101
BstDEI CTNAG 5 cut(s) 373, 492, 612, 1001, 1263
BstDSI CCRYGG 2 cut(s) 303, 1064
BstF5I GGATG 2 cut(s) 373, 459
BstFNI CGCG 2 cut(s) 166, 305
BstKTI GATC 5 cut(s) 11, 559, 889, 969, 1272
BstMAI GTCTC 3 cut(s) 488, 623, 980
BstMBI GATC 5 cut(s) 8, 556, 886, 966, 1269
BstMCI CGRYCG 2 cut(s) 170, 296
BstMWI GCNNNNNNNGC 4 cut(s) 605, 671, 860, 1100
BstSFI CTRYAG 2 cut(s) 117, 1244
BstUI CGCG 2 cut(s) 166, 305
BstV1I GCAGC 8 cut(s) 244, 608, 728, 797, 841, 1030, 1230, 1258
BstX2I RGATCY 2 cut(s) 8, 556
BstYI RGATCY 2 cut(s) 8, 556
BstZI CGGCCG 1 cut(s) 293
Bsu36I CCTNAGG 1 cut(s) 1001
BsuRI GGCC 2 cut(s) 102, 295
BtgI CCRYGG 2 cut(s) 303, 1064
BtgZI GCGATG 1 cut(s) 410
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 2 cut(s) 373, 459
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 1 cut(s) 672
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 3 cut(s) 456, 570, 1101
Cfr42I CCGCGG 1 cut(s) 306
Csp6I GTAC 1 cut(s) 552
CviAII CATG 6 cut(s) 79, 565, 581, 651, 964, 1065
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 5 cut(s) 373, 492, 612, 1001, 1263
DpnI GATC 5 cut(s) 10, 558, 888, 968, 1271
DpnII GATC 5 cut(s) 8, 556, 886, 966, 1269
EaeI YGGCCR 1 cut(s) 293
EagI CGGCCG 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 229
EciI GGCGGA 2 cut(s) 99, 271
EclXI CGGCCG 1 cut(s) 293
Eco130I CCWWGG 2 cut(s) 1020, 1064
Eco147I AGGCCT 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 980
Eco52I CGGCCG 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 834
Eco81I CCTNAGG 1 cut(s) 1001
EcoT14I CCWWGG 2 cut(s) 1020, 1064
EcoT22I ATGCAT 2 cut(s) 586, 907
ErhI CCWWGG 2 cut(s) 1020, 1064
FaeI CATG 6 cut(s) 82, 568, 584, 654, 967, 1068
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 1 cut(s) 89
FatI CATG 6 cut(s) 78, 564, 580, 650, 963, 1064
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 1 cut(s) 966
Fnu4HI GCNGC 9 cut(s) 258, 293, 597, 717, 786, 855, 1044, 1244, 1247
FokI GGATG 2 cut(s) 380, 446
Fsp4HI GCNGC 9 cut(s) 258, 293, 597, 717, 786, 855, 1044, 1244, 1247
FspBI CTAG 4 cut(s) 498, 521, 1047, 1091
GluI GCNGC 9 cut(s) 258, 293, 597, 717, 786, 855, 1044, 1244, 1247
HaeIII GGCC 2 cut(s) 102, 295
HapII CCGG 3 cut(s) 157, 170, 332
Hin1II CATG 6 cut(s) 82, 568, 584, 654, 967, 1068
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 7 cut(s) 221, 298, 308, 424, 536, 637, 996
HpaII CCGG 3 cut(s) 157, 170, 332
HphI GGTGA 2 cut(s) 397, 679
Hpy166II GTNNAC 1 cut(s) 1125
Hpy188I TCNGA 4 cut(s) 50, 376, 613, 886
Hpy188III TCNNGA 3 cut(s) 143, 620, 970
Hpy8I GTNNAC 1 cut(s) 1125
Hpy99I CGWCG 3 cut(s) 367, 695, 1191
HpyAV CCTTC 4 cut(s) 92, 147, 570, 967
HpyCH4III ACNGT 2 cut(s) 328, 1078
HpyCH4IV ACGT 2 cut(s) 843, 1319
HpyCH4V TGCA 7 cut(s) 119, 400, 584, 758, 857, 905, 1246
HpyF10VI GCNNNNNNNGC 4 cut(s) 605, 671, 860, 1100
HpyF3I CTNAG 5 cut(s) 373, 492, 612, 1001, 1263
HpySE526I ACGT 2 cut(s) 843, 1319
Hsp92II CATG 6 cut(s) 82, 568, 584, 654, 967, 1068
Ksp22I TGATCA 1 cut(s) 966
KspI CCGCGG 1 cut(s) 306
Kzo9I GATC 5 cut(s) 8, 556, 886, 966, 1269
LmnI GCTCC 4 cut(s) 51, 241, 247, 979
Lsp1109I GCAGC 8 cut(s) 244, 608, 728, 797, 841, 1030, 1230, 1258
LweI GCATC 4 cut(s) 358, 387, 595, 892
MaeI CTAG 4 cut(s) 498, 521, 1047, 1091
MaeII ACGT 2 cut(s) 843, 1319
MaeIII GTNAC 3 cut(s) 503, 844, 1207
MalI GATC 5 cut(s) 10, 558, 888, 968, 1271
MboI GATC 5 cut(s) 8, 556, 886, 966, 1269
MboII GAAGA 7 cut(s) 216, 219, 332, 415, 809, 833, 1279
MflI RGATCY 2 cut(s) 8, 556
MluCI AATT 4 cut(s) 147, 270, 641, 680
MlyI GAGTC 2 cut(s) 215, 418
Mph1103I ATGCAT 2 cut(s) 586, 907
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 3 cut(s) 644, 728, 911
MspA1I CMGCKG 5 cut(s) 305, 719, 788, 854, 863
MspI CCGG 3 cut(s) 157, 170, 332
Mva1269I GAATGC 1 cut(s) 1275
MvnI CGCG 2 cut(s) 166, 305
MwoI GCNNNNNNNGC 4 cut(s) 605, 671, 860, 1100
NcoI CCATGG 1 cut(s) 1064
NdeII GATC 5 cut(s) 8, 556, 886, 966, 1269
NlaIII CATG 6 cut(s) 82, 568, 584, 654, 967, 1068
NlaIV GGNNCC 3 cut(s) 174, 558, 1070
NmuCI GTSAC 1 cut(s) 844
NsiI ATGCAT 2 cut(s) 586, 907
PaqCI CACCTGC 1 cut(s) 262
PceI AGGCCT 1 cut(s) 102
PctI GAATGC 1 cut(s) 1275
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 5 cut(s) 298, 308, 536, 637, 996
PkrI GCNGC 9 cut(s) 259, 294, 598, 718, 787, 856, 1045, 1245, 1248
PleI GAGTC 2 cut(s) 215, 418
PpsI GAGTC 2 cut(s) 215, 418
PsiI TTATAA 1 cut(s) 1056
PspN4I GGNNCC 3 cut(s) 174, 558, 1070
PstI CTGCAG 2 cut(s) 121, 1248
PsuI RGATCY 2 cut(s) 8, 556
PvuII CAGCTG 3 cut(s) 719, 788, 854
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
SacII CCGCGG 1 cut(s) 306
SaqAI TTAA 3 cut(s) 644, 728, 911
SatI GCNGC 9 cut(s) 258, 293, 597, 717, 786, 855, 1044, 1244, 1247
Sau3AI GATC 5 cut(s) 8, 556, 886, 966, 1269
SchI GAGTC 2 cut(s) 215, 418
SfaNI GCATC 4 cut(s) 358, 387, 595, 892
SfcI CTRYAG 2 cut(s) 117, 1244
Sfr303I CCGCGG 1 cut(s) 306
SgrBI CCGCGG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 497
Sse9I AATT 4 cut(s) 147, 270, 641, 680
SseBI AGGCCT 1 cut(s) 102
SsiI CCGC 8 cut(s) 110, 164, 282, 292, 303, 305, 863, 1299
SspI AATATT 1 cut(s) 1256
SspMI CTAG 4 cut(s) 498, 521, 1047, 1091
StuI AGGCCT 1 cut(s) 102
StyI CCWWGG 2 cut(s) 1020, 1064
TaaI ACNGT 2 cut(s) 328, 1078
TaiI ACGT 2 cut(s) 846, 1322
TaqI TCGA 6 cut(s) 59, 196, 311, 621, 1189, 1236
TasI AATT 4 cut(s) 147, 270, 641, 680
TauI GCSGC 1 cut(s) 295
TfiI GAWTC 5 cut(s) 298, 308, 536, 637, 996
Tru1I TTAA 3 cut(s) 644, 728, 911
Tru9I TTAA 3 cut(s) 644, 728, 911
TscAI CASTG 1 cut(s) 672
TseFI GTSAC 1 cut(s) 844
TseI GCWGC 8 cut(s) 257, 596, 716, 785, 854, 1043, 1243, 1246
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 3 cut(s) 693, 834, 848
TspGWI ACGGA 2 cut(s) 380, 569
TspRI CASTG 1 cut(s) 672
XapI RAATTY 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 55
XspI CTAG 4 cut(s) 498, 521, 1047, 1091
Zsp2I ATGCAT 2 cut(s) 586, 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.