RLG00000025760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014542
Physical Location & Seq
Forward (+)
48118772 .. 48123067
4296 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025760

Sequence Viewer

Length: 1275 bp
ATGGCGGACGACCTGTTTGAAGGTCTGCCGCCGCCTTCTTCTGCAAACCCTCTTCCCTCTTCTGAGCCTCAAGAACATGAAAAACAACCCAAACCCAAACCAAAGTTCTCTTCTTTCAGCACCGACTCTTCTTCTTCTTCACCTTCTCCTTCTCAAGCTCCACAGCCTGCTCTAAAAAGCGCATTGAAGAAGCCTAAGGAGCCCTCTGAGCCAAACCCAGTTCTCAAACGGCCCAAGGAGCCCTCTGAACCAAACCCAGAAGCCACTGTGCCTGGAAAGAAGTTGAGGTTTAAAACCACGACCGATGCCTCAGAGAAACAGGTTATAGAGGCCATGCAGAAGATAGCTTCACATATAAAGAATCCTACAAAGTTTGGGAAGGCTTCAAAGCTTGCCATCCAGCTGATTCAGGCAGGAAGTGTCAAGGCCGAAACCAGTGATTACTTCTTTTCAATATTAGAGGCTGCAATGACATCATCTACCTCTTGCACGGATCCTTCCATACGAACAGATATCCATGCACTCTTCTCAGCAGCACAAGATGCCTCTGAGTGTCTCAATAAGAAGCAAAAGAATCAGTTAACAACATGGACAATCAGAGCTGTGATGGCAAATGATTTACTCACTGATGACAGCTTCCTGTTTTCAAAAACAGCTGGACAATTAAAAGAAGAAATATCTAATCTGCCAGTTGCAACTGAGGATGATGACAGAGAAGAAGCAGCTGCCCTGGAAGTTGAAACTAAAACAACTGATGAGGATGATCTAATGAAGAAGGACATGACTTCGGATGTACCGGATGAAGAAAATAATGAGAAAGAGTCTGATCCATTTGGGCTAGATGCATTTTTAACTCCAAGCTCTATGAAGAAAGGTGATAAAGTAAAGGGAAAGAAAGATGTAACTCCCAAAGTCAAGGAGGAGGAAGAGACCAAGAGATTCCTCAAGTCACAGAGAGAAGCCTTGGTCCTTTGTTTAGAGATAGCTGCTCGGCGTTATAAAACTCCATGGTGCCAGACAGTTATAGACATATTAGCAAAGCATGCTTTTGATAACATAAGGAGGTTCACATCTGAACAAAGGAATGCCATTGGGAAACTTTGGGCTTCCATACGGGAACAGCATAATCGTAGAAAACAAGGGAAAACAGTTACTGGGAAACTTGACGTGAATGGTTTCGAATGGCTGCAGCAAAAATATGCTAATGAGAAGATCAGCATTCGGCATTCTGTTGGCGGTAGTGGGGATCGTAAAACTGAAATGTGGCTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

425

Amino Acids

47.17

Weight (kDa)

7.03

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014542)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04560
fragaria_vesca FvH4_6g02310
malus_domestica MD04G1228300.v1.1 MD04G1228400.v1.1 MD12G1245500.v1.1
prunus_persica Prupe.6G348300_v2.0.a1
pyrus_communis pycom12g22500
rosa_chinensis RchiOBHm_Chr3g0450061
rosa_laevigata RLG00000025760
rosa_multiflora Rmu_sc0002646.1_g000024
rosa_roxburghii Rroxscaffold_6G00427840
rosa_rugosa Rorug02G0625200
rosa_samantha Rh3AG024300 Rh3BG024900 Rh3CG023800 Rh3DG024900
rosa_wichuraiana Rw3G001940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 999
AccB1I GGYRCC 1 cut(s) 1011
AciI CCGC 4 cut(s) 5, 29, 32, 1236
AclWI GGATC 4 cut(s) 488, 501, 821, 1254
AfaI GTAC 1 cut(s) 795
AgsI TTSAA 6 cut(s) 20, 187, 387, 453, 648, 740
AjiI CACGTC 1 cut(s) 1168
AjnI CCWGG 2 cut(s) 271, 729
AjuI GAANNNNNNNTTGG 5 cut(s) 89, 94, 121, 126, 1251
Alw26I GTCTC 2 cut(s) 560, 923
AlwI GGATC 4 cut(s) 488, 501, 821, 1254
AlwNI CAGNNNCTG 1 cut(s) 1154
AoxI GGCC 3 cut(s) 230, 330, 426
ApeKI GCWGC 7 cut(s) 464, 533, 722, 725, 986, 1186, 1189
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 1 cut(s) 1175
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 2 cut(s) 231, 967
AsuHPI GGTGA 2 cut(s) 132, 887
AsuII TTCGAA 1 cut(s) 1179
AvaII GGWCC 1 cut(s) 967
AxyI CCTNAGG 1 cut(s) 195
BamHI GGATCC 1 cut(s) 493
BanI GGYRCC 1 cut(s) 1011
BanII GRGCYC 2 cut(s) 204, 243
BbvI GCAGC 7 cut(s) 451, 545, 712, 734, 973, 1173, 1201
BccI CCATC 2 cut(s) 404, 601
BceAI ACGGC 1 cut(s) 245
BciT130I CCWGG 2 cut(s) 273, 731
BcoDI GTCTC 2 cut(s) 560, 923
BfaI CTAG 1 cut(s) 839
BfmI CTRYAG 1 cut(s) 1187
BisI GCNGC 9 cut(s) 29, 32, 465, 534, 723, 726, 987, 1187, 1190
BlsI GCNGC 9 cut(s) 30, 33, 466, 535, 724, 727, 988, 1188, 1191
Bme1390I CCNGG 2 cut(s) 273, 731
Bme18I GGWCC 1 cut(s) 967
BmgBI CACGTC 1 cut(s) 1168
BmgT120I GGNCC 2 cut(s) 231, 967
BmiI GGNNCC 4 cut(s) 201, 240, 495, 1013
BmrFI CCNGG 2 cut(s) 273, 731
BmrI ACTGGG 2 cut(s) 212, 1164
BmsI GCATC 3 cut(s) 295, 532, 832
BmuI ACTGGG 2 cut(s) 212, 1164
Bpu14I TTCGAA 1 cut(s) 1179
BpuEI CTTGAG 3 cut(s) 54, 138, 929
BsaI GGTCTC 1 cut(s) 923
BsaJI CCNNGG 4 cut(s) 234, 729, 963, 1007
BsaWI WCCGGW 1 cut(s) 796
Bse1I ACTGG 4 cut(s) 218, 435, 689, 1159
Bse21I CCTNAGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 2 cut(s) 273, 731
BseDI CCNNGG 4 cut(s) 234, 729, 963, 1007
BseGI GGATG 5 cut(s) 396, 709, 766, 796, 805
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 6 cut(s) 54, 198, 324, 540, 543, 690
BseNI ACTGG 4 cut(s) 218, 435, 689, 1159
BseRI GAGGAG 1 cut(s) 935
BseXI GCAGC 7 cut(s) 451, 545, 712, 734, 973, 1173, 1201
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 3 cut(s) 232, 332, 428
BshNI GGYRCC 1 cut(s) 1011
BsiEI CGRYCG 1 cut(s) 303
BsiSI CCGG 1 cut(s) 797
BsmAI GTCTC 2 cut(s) 560, 923
BsmI GAATGC 3 cut(s) 1090, 1218, 1225
BsnI GGCC 3 cut(s) 232, 332, 428
Bso31I GGTCTC 1 cut(s) 923
Bsp119I TTCGAA 1 cut(s) 1179
Bsp1286I GDGCHC 2 cut(s) 204, 243
Bsp143I GATC 5 cut(s) 493, 763, 826, 1212, 1246
Bsp19I CCATGG 1 cut(s) 1007
BspACI CCGC 4 cut(s) 5, 29, 32, 1236
BspANI GGCC 3 cut(s) 232, 332, 428
BspCNI CTCAG 6 cut(s) 55, 199, 323, 541, 542, 691
BspLI GGNNCC 4 cut(s) 201, 240, 495, 1013
BspMAI CTGCAG 1 cut(s) 1191
BspPI GGATC 4 cut(s) 488, 501, 821, 1254
BspT104I TTCGAA 1 cut(s) 1179
BspT107I GGYRCC 1 cut(s) 1011
BspTNI GGTCTC 1 cut(s) 923
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 4 cut(s) 218, 435, 689, 1159
BssECI CCNNGG 4 cut(s) 234, 729, 963, 1007
BssMI GATC 5 cut(s) 493, 763, 826, 1212, 1246
BssT1I CCWWGG 3 cut(s) 234, 963, 1007
Bst2UI CCWGG 2 cut(s) 273, 731
Bst4CI ACNGT 3 cut(s) 268, 1021, 1150
Bst6I CTCTTC 6 cut(s) 57, 64, 115, 133, 530, 921
BstAPI GCANNNNNTGC 2 cut(s) 542, 1043
BstBI TTCGAA 1 cut(s) 1179
BstC8I GCNNGC 3 cut(s) 168, 393, 1044
BstDEI CTNAG 7 cut(s) 63, 195, 207, 310, 529, 549, 699
BstDSI CCRYGG 1 cut(s) 1007
BstF5I GGATG 5 cut(s) 396, 709, 766, 796, 805
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 5 cut(s) 496, 766, 829, 1215, 1249
BstMAI GTCTC 2 cut(s) 560, 923
BstMBI GATC 5 cut(s) 493, 763, 826, 1212, 1246
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 6 cut(s) 199, 208, 238, 542, 608, 1043
BstNI CCWGG 2 cut(s) 273, 731
BstNSI RCATGY 1 cut(s) 1046
BstSCI CCNGG 2 cut(s) 271, 729
BstSFI CTRYAG 1 cut(s) 1187
BstV1I GCAGC 7 cut(s) 451, 545, 712, 734, 973, 1173, 1201
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
Bsu36I CCTNAGG 1 cut(s) 195
BsuRI GGCC 3 cut(s) 232, 332, 428
BtgI CCRYGG 1 cut(s) 1007
BtrI CACGTC 1 cut(s) 1168
BtsCI GGATG 5 cut(s) 396, 709, 766, 796, 805
BtsIMutI CAGTG 3 cut(s) 264, 442, 624
Cac8I GCNNGC 3 cut(s) 168, 393, 1044
CaiI CAGNNNCTG 1 cut(s) 1154
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 231, 967
Csp6I GTAC 1 cut(s) 794
CviAII CATG 7 cut(s) 77, 334, 518, 588, 781, 1008, 1043
CviQI GTAC 1 cut(s) 794
DdeI CTNAG 7 cut(s) 63, 195, 207, 310, 529, 549, 699
DpnI GATC 5 cut(s) 495, 765, 828, 1214, 1248
DpnII GATC 5 cut(s) 493, 763, 826, 1212, 1246
DraI TTTAAA 1 cut(s) 292
Eam1104I CTCTTC 6 cut(s) 57, 64, 115, 133, 530, 921
EarI CTCTTC 6 cut(s) 57, 64, 115, 133, 530, 921
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 234, 963, 1007
Eco24I GRGCYC 2 cut(s) 204, 243
Eco31I GGTCTC 1 cut(s) 923
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 967
Eco81I CCTNAGG 1 cut(s) 195
EcoRII CCWGG 2 cut(s) 271, 729
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 3 cut(s) 234, 963, 1007
EcoT22I ATGCAT 1 cut(s) 847
EcoT38I GRGCYC 2 cut(s) 204, 243
ErhI CCWWGG 3 cut(s) 234, 963, 1007
FaeI CATG 7 cut(s) 80, 337, 521, 591, 784, 1011, 1046
FatI CATG 7 cut(s) 76, 333, 517, 587, 780, 1007, 1042
Fnu4HI GCNGC 9 cut(s) 29, 32, 465, 534, 723, 726, 987, 1187, 1190
FokI GGATG 5 cut(s) 383, 716, 773, 803, 812
FriOI GRGCYC 2 cut(s) 204, 243
Fsp4HI GCNGC 9 cut(s) 29, 32, 465, 534, 723, 726, 987, 1187, 1190
FspBI CTAG 1 cut(s) 839
GlaI GCGC 1 cut(s) 181
GluI GCNGC 9 cut(s) 29, 32, 465, 534, 723, 726, 987, 1187, 1190
HaeIII GGCC 3 cut(s) 232, 332, 428
HapII CCGG 1 cut(s) 797
HhaI GCGC 1 cut(s) 182
Hin1II CATG 7 cut(s) 80, 337, 521, 591, 784, 1011, 1046
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 6 cut(s) 125, 361, 406, 574, 821, 939
HpaI GTTAAC 1 cut(s) 582
HpaII CCGG 1 cut(s) 797
HphI GGTGA 2 cut(s) 132, 887
Hpy166II GTNNAC 2 cut(s) 582, 1068
Hpy188I TCNGA 9 cut(s) 64, 208, 247, 313, 550, 599, 790, 826, 1075
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 2 cut(s) 582, 1068
HpyAV CCTTC 7 cut(s) 14, 45, 153, 159, 373, 507, 769
HpyCH4III ACNGT 3 cut(s) 268, 1021, 1150
HpyCH4IV ACGT 1 cut(s) 1167
HpyCH4V TGCA 8 cut(s) 44, 337, 467, 489, 521, 695, 845, 1189
HpyF10VI GCNNNNNNNGC 6 cut(s) 199, 208, 238, 542, 608, 1043
HpyF3I CTNAG 7 cut(s) 63, 195, 207, 310, 529, 549, 699
HpySE526I ACGT 1 cut(s) 1167
Hsp92II CATG 7 cut(s) 80, 337, 521, 591, 784, 1011, 1046
HspAI GCGC 1 cut(s) 180
KspAI GTTAAC 1 cut(s) 582
Kzo9I GATC 5 cut(s) 493, 763, 826, 1212, 1246
LmnI GCTCC 3 cut(s) 163, 199, 238
Lsp1109I GCAGC 7 cut(s) 451, 545, 712, 734, 973, 1173, 1201
LweI GCATC 3 cut(s) 295, 532, 832
MaeI CTAG 1 cut(s) 839
MaeII ACGT 1 cut(s) 1167
MaeIII GTNAC 3 cut(s) 901, 948, 1150
MalI GATC 5 cut(s) 495, 765, 828, 1214, 1248
MboI GATC 5 cut(s) 493, 763, 826, 1212, 1246
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 2 cut(s) 204, 243
MluCI AATT 1 cut(s) 662
MlyI GAGTC 2 cut(s) 119, 830
Mph1103I ATGCAT 1 cut(s) 847
MroXI GAANNNNTTC 1 cut(s) 1175
MseI TTAA 4 cut(s) 291, 581, 665, 851
MspA1I CMGCKG 3 cut(s) 403, 656, 725
MspI CCGG 1 cut(s) 797
MspR9I CCNGG 2 cut(s) 273, 731
Mva1269I GAATGC 3 cut(s) 1090, 1218, 1225
MvaI CCWGG 2 cut(s) 273, 731
MwoI GCNNNNNNNGC 6 cut(s) 199, 208, 238, 542, 608, 1043
NcoI CCATGG 1 cut(s) 1007
NdeII GATC 5 cut(s) 493, 763, 826, 1212, 1246
NlaIII CATG 7 cut(s) 80, 337, 521, 591, 784, 1011, 1046
NlaIV GGNNCC 4 cut(s) 201, 240, 495, 1013
NmeAIII GCCGAG 1 cut(s) 970
NmuCI GTSAC 1 cut(s) 948
NsiI ATGCAT 1 cut(s) 847
NspI RCATGY 1 cut(s) 1046
NspV TTCGAA 1 cut(s) 1179
PaeI GCATGC 1 cut(s) 1046
PctI GAATGC 3 cut(s) 1090, 1218, 1225
PdmI GAANNNNTTC 1 cut(s) 1175
PfeI GAWTC 4 cut(s) 361, 406, 574, 939
PkrI GCNGC 9 cut(s) 30, 33, 466, 535, 724, 727, 988, 1188, 1191
PleI GAGTC 2 cut(s) 119, 829
PpsI GAGTC 2 cut(s) 119, 829
PsiI TTATAA 1 cut(s) 999
Psp6I CCWGG 2 cut(s) 271, 729
PspGI CCWGG 2 cut(s) 271, 729
PspN4I GGNNCC 4 cut(s) 201, 240, 495, 1013
PspPI GGNCC 2 cut(s) 231, 967
PstI CTGCAG 1 cut(s) 1191
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 1 cut(s) 493
PvuII CAGCTG 3 cut(s) 403, 656, 725
RsaI GTAC 1 cut(s) 795
RsaNI GTAC 1 cut(s) 794
SaqAI TTAA 4 cut(s) 291, 581, 665, 851
SatI GCNGC 9 cut(s) 29, 32, 465, 534, 723, 726, 987, 1187, 1190
Sau3AI GATC 5 cut(s) 493, 763, 826, 1212, 1246
Sau96I GGNCC 2 cut(s) 231, 967
SchI GAGTC 2 cut(s) 119, 830
ScrFI CCNGG 2 cut(s) 273, 731
SduI GDGCHC 2 cut(s) 204, 243
SfaNI GCATC 3 cut(s) 295, 532, 832
SfcI CTRYAG 1 cut(s) 1187
SfuI TTCGAA 1 cut(s) 1179
SinI GGWCC 1 cut(s) 967
SmlI CTYRAG 3 cut(s) 69, 153, 944
SmoI CTYRAG 3 cut(s) 69, 153, 944
SphI GCATGC 1 cut(s) 1046
Sse9I AATT 1 cut(s) 662
SsiI CCGC 4 cut(s) 5, 29, 32, 1236
SspI AATATT 1 cut(s) 456
SspMI CTAG 1 cut(s) 839
StyD4I CCNGG 2 cut(s) 271, 729
StyI CCWWGG 3 cut(s) 234, 963, 1007
TaaI ACNGT 3 cut(s) 268, 1021, 1150
TaiI ACGT 1 cut(s) 1170
TaqI TCGA 1 cut(s) 1179
TaqII GACCGA 1 cut(s) 317
TasI AATT 1 cut(s) 662
TauI GCSGC 2 cut(s) 31, 34
TfiI GAWTC 4 cut(s) 361, 406, 574, 939
Tru1I TTAA 4 cut(s) 291, 581, 665, 851
Tru9I TTAA 4 cut(s) 291, 581, 665, 851
TscAI CASTG 3 cut(s) 271, 442, 631
TseFI GTSAC 1 cut(s) 948
TseI GCWGC 7 cut(s) 464, 533, 722, 725, 986, 1186, 1189
Tsp45I GTSAC 1 cut(s) 948
TspDTI ATGAA 4 cut(s) 93, 785, 816, 881
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 3 cut(s) 271, 442, 631
VpaK11BI GGWCC 1 cut(s) 967
XceI RCATGY 1 cut(s) 1046
XmnI GAANNNNTTC 1 cut(s) 1175
XspI CTAG 1 cut(s) 839
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.