MD12G1245500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014542
Physical Location & Seq
Forward (+)
31595927 .. 31598293
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1245500.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGGCTGACGACCTGTTTGAAGGCCTCCCTCCGCCATCTGCAAACCCTCCTCCTCCTCCTTCTCCTGAAATCCAACTCCGGCAACAGCTGCCGGAACCACAAAAGCCACCAAAACCGCGAAAAACAGCTTCTTTTAGCTGCGACTCTTCTTCTTCCCCTGCTCCTGCTCCTCCTCCAGTTGCTGCCCCTAAACCAATTCTCAAATCCGCCCTCAAGCGGCCGAATCCCGCCGAGTCGACCCCAGAAGAAACTGTGCCGGAAAAGAAAAAGTTGAGGTTCAAAACCACGACGGATGCCTCAGAGCAACAGGTGATAGAGGCGATGCAGAAGATAGCTTCGCATATAAAGACTCCTGCGAAGTTTAGTAAGGCTTCAAAGCTTGCCATCCAGCTTATTCAGGCGGGAAGTGTGAAAGCTGAGACTAGTGATTACTGTTTTGCTATACTAGAAGCCGCAATGGAATCTCCTACTTCTTGTACGGATCCTTCCATGCGAGCTGATTACCATGCATTGTTTTCAGCGGCACAAGATGCCTCTGAGTGTCTCGATAAGAAGCAAAAGAATCAATTAACAACATGGACAATCAGGGCAGTGATGGCAAATGAATTATTCACCGACGACAGCTTCCTGTTTTCAAAAGCAGCTGGACGATTAAAGGAAGCCATATCTAATCTGCCTGTTGCAACCGAGGACGATGACAGAGAGGAAGCAGCTGTGCTGGAAGATGGAACAAAGATAGCAGATGAAGATGATCAGACGAAGCAGGATGTGACTTCAGCTGCACCAGCGGAAAATAATGATGAGTCATCTGATCCGTTTGGGCTTGATGCATTTTTAACTCCTGCCTCTACAAAGAAAGATGATAGAACAAAGGAAAAAAAAGATAACAAGATCACGAAGGAGGAGGAAGAGACCAAGAGATTCCTCAGATCAAAAAGAGAAGCCTTGGTTCTTTGTTTAGAGATAGCTGCTCAGCGTTATAAAACCCCGTGGTGCCAAACAGTCATAGACATATTAGCAAAGCACGCCTTTGATAACATAGCAAGGTTCACATTGAAACAAAGGACTGCCATTGAGAAACTTTGGGTTTCCATACGGGAACAGCATAATCGTCGAAAACAAGGGAAATCGATTACTGGGAAACTTGATGTCAACGCTTTCGAGTGGCTGCAGCAAAAATATTCTACTGAGAAGATCAGCATTCGGCACTCTGTAAGTGGTAGCGGAGGTCGTAAAACTGAAACTTGGCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

419

Amino Acids

46.34

Weight (kDa)

6.98

Isoelectric Point (pI)

60.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014542)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04560
fragaria_vesca FvH4_6g02310
malus_domestica MD04G1228300.v1.1 MD04G1228400.v1.1 MD12G1245500.v1.1
prunus_persica Prupe.6G348300_v2.0.a1
pyrus_communis pycom12g22500
rosa_chinensis RchiOBHm_Chr3g0450061
rosa_laevigata RLG00000025760
rosa_multiflora Rmu_sc0002646.1_g000024
rosa_roxburghii Rroxscaffold_6G00427840
rosa_rugosa Rorug02G0625200
rosa_samantha Rh3AG024300 Rh3BG024900 Rh3CG023800 Rh3DG024900
rosa_wichuraiana Rw3G001940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AccB1I GGYRCC 1 cut(s) 993
AccI GTMKAC 1 cut(s) 236
AccII CGCG 1 cut(s) 118
AclWI GGATC 3 cut(s) 476, 489, 806
AcoI YGGCCR 1 cut(s) 218
AcuI CTGAAG 1 cut(s) 759
AfaI GTAC 1 cut(s) 478
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 5 cut(s) 20, 280, 375, 636, 1057
AhlI ACTAGT 1 cut(s) 422
AjuI GAANNNNNNNTTGG 1 cut(s) 1233
Alw26I GTCTC 3 cut(s) 413, 548, 905
AlwI GGATC 3 cut(s) 476, 489, 806
AlwNI CAGNNNCTG 1 cut(s) 182
AoxI GGCC 2 cut(s) 22, 218
ApeKI GCWGC 9 cut(s) 88, 138, 182, 641, 710, 779, 968, 1168, 1171
ArsI GACNNNNNNTTYG 1 cut(s) 31
AsuHPI GGTGA 2 cut(s) 322, 604
BamHI GGATCC 1 cut(s) 481
BanI GGYRCC 1 cut(s) 993
BarI GAAGNNNNNNTAC 2 cut(s) 469, 501
BbvI GCAGC 9 cut(s) 75, 125, 169, 653, 722, 766, 955, 1155, 1183
BccI CCATC 4 cut(s) 43, 392, 589, 719
BcgI CGANNNNNNTGC 2 cut(s) 1094, 1128
BclI TGATCA 1 cut(s) 751
BcoDI GTCTC 3 cut(s) 413, 548, 905
BcuI ACTAGT 1 cut(s) 422
BfaI CTAG 2 cut(s) 423, 446
BfmI CTRYAG 1 cut(s) 1169
BlpI GCTNAGC 1 cut(s) 972
BmiI GGNNCC 3 cut(s) 96, 483, 995
BmrI ACTGGG 1 cut(s) 1146
BmsI GCATC 4 cut(s) 283, 312, 520, 817
BmuI ACTGGG 1 cut(s) 1146
BpmI CTGGAG 1 cut(s) 159
Bpu1102I GCTNAGC 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 197
Bsa29I ATCGAT 1 cut(s) 1130
BsaI GGTCTC 1 cut(s) 905
BsaJI CCNNGG 3 cut(s) 687, 945, 989
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse1I ACTGG 2 cut(s) 176, 1141
Bse3DI GCAATG 1 cut(s) 462
BseCI ATCGAT 1 cut(s) 1130
BseDI CCNNGG 3 cut(s) 687, 945, 989
BseGI GGATG 3 cut(s) 298, 384, 772
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 6 cut(s) 312, 408, 528, 940, 986, 1179
BseNI ACTGG 2 cut(s) 176, 1141
BseRI GAGGAG 6 cut(s) 39, 42, 45, 159, 162, 917
BseX3I CGGCCG 1 cut(s) 218
BseXI GCAGC 9 cut(s) 75, 125, 169, 653, 722, 766, 955, 1155, 1183
BsgI GTGCAG 1 cut(s) 765
Bsh1236I CGCG 1 cut(s) 118
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 2 cut(s) 24, 220
BshNI GGYRCC 1 cut(s) 993
BshVI ATCGAT 1 cut(s) 1130
BsiEI CGRYCG 1 cut(s) 221
BsiSI CCGG 3 cut(s) 79, 92, 257
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 3 cut(s) 413, 548, 905
BsmI GAATGC 1 cut(s) 1200
BsnI GGCC 2 cut(s) 24, 220
Bso31I GGTCTC 1 cut(s) 905
Bsp143I GATC 6 cut(s) 481, 751, 811, 891, 929, 1194
Bsp1720I GCTNAGC 1 cut(s) 972
BspANI GGCC 2 cut(s) 24, 220
BspCNI CTCAG 6 cut(s) 311, 409, 529, 939, 985, 1180
BspDI ATCGAT 1 cut(s) 1130
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 3 cut(s) 96, 483, 995
BspMAI CTGCAG 1 cut(s) 1173
BspPI GGATC 3 cut(s) 476, 489, 806
BspT107I GGYRCC 1 cut(s) 993
BspTNI GGTCTC 1 cut(s) 905
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 2 cut(s) 176, 1141
BssECI CCNNGG 3 cut(s) 687, 945, 989
BssMI GATC 6 cut(s) 481, 751, 811, 891, 929, 1194
BssT1I CCWWGG 1 cut(s) 945
Bst4CI ACNGT 3 cut(s) 253, 434, 1003
Bst6I CTCTTC 2 cut(s) 151, 903
BstAPI GCANNNNNTGC 2 cut(s) 88, 530
BstC8I GCNNGC 3 cut(s) 381, 495, 1026
BstDEI CTNAG 6 cut(s) 298, 417, 537, 926, 972, 1188
BstDSI CCRYGG 1 cut(s) 989
BstF5I GGATG 3 cut(s) 298, 384, 772
BstFNI CGCG 1 cut(s) 118
BstKTI GATC 6 cut(s) 484, 754, 814, 894, 932, 1197
BstMAI GTCTC 3 cut(s) 413, 548, 905
BstMBI GATC 6 cut(s) 481, 751, 811, 891, 929, 1194
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 5 cut(s) 88, 530, 596, 785, 1025
BstSFI CTRYAG 1 cut(s) 1169
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 9 cut(s) 75, 125, 169, 653, 722, 766, 955, 1155, 1183
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 1 cut(s) 218
Bsu15I ATCGAT 1 cut(s) 1130
BsuRI GGCC 2 cut(s) 24, 220
BsuTUI ATCGAT 1 cut(s) 1130
BtgI CCRYGG 1 cut(s) 989
BtgZI GCGATG 1 cut(s) 335
BtsCI GGATG 3 cut(s) 298, 384, 772
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 1 cut(s) 597
Cac8I GCNNGC 3 cut(s) 381, 495, 1026
CaiI CAGNNNCTG 1 cut(s) 182
ClaI ATCGAT 1 cut(s) 1130
Csp6I GTAC 1 cut(s) 477
CviAII CATG 3 cut(s) 490, 506, 576
CviQI GTAC 1 cut(s) 477
DdeI CTNAG 6 cut(s) 298, 417, 537, 926, 972, 1188
DpnI GATC 6 cut(s) 483, 753, 813, 893, 931, 1196
DpnII GATC 6 cut(s) 481, 751, 811, 891, 929, 1194
EaeI YGGCCR 1 cut(s) 218
EagI CGGCCG 1 cut(s) 218
Eam1104I CTCTTC 2 cut(s) 151, 903
EarI CTCTTC 2 cut(s) 151, 903
EciI GGCGGA 2 cut(s) 21, 196
EclXI CGGCCG 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 945
Eco147I AGGCCT 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 905
Eco52I CGGCCG 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 945
EcoT22I ATGCAT 2 cut(s) 511, 832
ErhI CCWWGG 1 cut(s) 945
FaeI CATG 3 cut(s) 493, 509, 579
FalI AAGNNNNNCTT 2 cut(s) 1013, 1045
FatI CATG 3 cut(s) 489, 505, 575
FauI CCCGC 2 cut(s) 235, 394
FbaI TGATCA 1 cut(s) 751
FblI GTMKAC 1 cut(s) 236
FokI GGATG 3 cut(s) 305, 371, 779
FspBI CTAG 2 cut(s) 423, 446
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 2 cut(s) 24, 220
HapII CCGG 3 cut(s) 79, 92, 257
Hin1II CATG 3 cut(s) 493, 509, 579
HincII GTYRAC 2 cut(s) 237, 1153
HindII GTYRAC 2 cut(s) 237, 1153
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 8 cut(s) 143, 223, 233, 349, 461, 562, 803, 921
HpaII CCGG 3 cut(s) 79, 92, 257
HphI GGTGA 2 cut(s) 322, 604
Hpy166II GTNNAC 3 cut(s) 237, 1050, 1153
Hpy188I TCNGA 5 cut(s) 301, 538, 756, 811, 929
Hpy188III TCNNGA 3 cut(s) 65, 545, 895
Hpy8I GTNNAC 3 cut(s) 237, 1050, 1153
Hpy99I CGWCG 3 cut(s) 292, 620, 1116
HpyAV CCTTC 4 cut(s) 14, 69, 495, 892
HpyCH4III ACNGT 3 cut(s) 253, 434, 1003
HpyCH4V TGCA 7 cut(s) 41, 325, 509, 683, 782, 830, 1171
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 530, 596, 785, 1025
HpyF3I CTNAG 6 cut(s) 298, 417, 537, 926, 972, 1188
Hsp92II CATG 3 cut(s) 493, 509, 579
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 6 cut(s) 481, 751, 811, 891, 929, 1194
LmnI GCTCC 2 cut(s) 166, 172
Lsp1109I GCAGC 9 cut(s) 75, 125, 169, 653, 722, 766, 955, 1155, 1183
LweI GCATC 4 cut(s) 283, 312, 520, 817
MaeI CTAG 2 cut(s) 423, 446
MaeIII GTNAC 1 cut(s) 769
MalI GATC 6 cut(s) 483, 753, 813, 893, 931, 1196
MboI GATC 6 cut(s) 481, 751, 811, 891, 929, 1194
MboII GAAGA 9 cut(s) 138, 141, 144, 257, 340, 734, 758, 920, 1204
MflI RGATCY 1 cut(s) 481
MluCI AATT 3 cut(s) 195, 566, 605
MlyI GAGTC 4 cut(s) 137, 242, 343, 812
MmeI TCCRAC 1 cut(s) 97
Mph1103I ATGCAT 2 cut(s) 511, 832
MseI TTAA 3 cut(s) 569, 653, 836
MspA1I CMGCKG 6 cut(s) 88, 521, 644, 713, 779, 788
MspI CCGG 3 cut(s) 79, 92, 257
Mva1269I GAATGC 1 cut(s) 1200
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 5 cut(s) 88, 530, 596, 785, 1025
NdeII GATC 6 cut(s) 481, 751, 811, 891, 929, 1194
NlaIII CATG 3 cut(s) 493, 509, 579
NlaIV GGNNCC 3 cut(s) 96, 483, 995
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 769
NsiI ATGCAT 2 cut(s) 511, 832
PceI AGGCCT 1 cut(s) 24
PctI GAATGC 1 cut(s) 1200
PfeI GAWTC 4 cut(s) 223, 461, 562, 921
PleI GAGTC 4 cut(s) 137, 241, 343, 811
PpsI GAGTC 4 cut(s) 137, 241, 343, 811
PsiI TTATAA 1 cut(s) 981
PspN4I GGNNCC 3 cut(s) 96, 483, 995
PstI CTGCAG 1 cut(s) 1173
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 481
PvuII CAGCTG 4 cut(s) 88, 644, 713, 779
RsaI GTAC 1 cut(s) 478
RsaNI GTAC 1 cut(s) 477
SalI GTCGAC 1 cut(s) 235
SaqAI TTAA 3 cut(s) 569, 653, 836
Sau3AI GATC 6 cut(s) 481, 751, 811, 891, 929, 1194
SchI GAGTC 4 cut(s) 137, 242, 343, 812
SfaNI GCATC 4 cut(s) 283, 312, 520, 817
SfcI CTRYAG 1 cut(s) 1169
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SpeI ACTAGT 1 cut(s) 422
Sse9I AATT 3 cut(s) 195, 566, 605
SseBI AGGCCT 1 cut(s) 24
SspI AATATT 1 cut(s) 1181
SspMI CTAG 2 cut(s) 423, 446
StuI AGGCCT 1 cut(s) 24
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 3 cut(s) 253, 434, 1003
TaqI TCGA 5 cut(s) 236, 546, 1114, 1130, 1161
TasI AATT 3 cut(s) 195, 566, 605
TauI GCSGC 3 cut(s) 220, 455, 524
TfiI GAWTC 4 cut(s) 223, 461, 562, 921
Tru1I TTAA 3 cut(s) 569, 653, 836
Tru9I TTAA 3 cut(s) 569, 653, 836
TscAI CASTG 1 cut(s) 597
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 9 cut(s) 88, 138, 182, 641, 710, 779, 968, 1168, 1171
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 2 cut(s) 618, 759
TspGWI ACGGA 3 cut(s) 305, 494, 804
TspRI CASTG 1 cut(s) 597
XmiI GTMKAC 1 cut(s) 236
XspI CTAG 2 cut(s) 423, 446
Zsp2I ATGCAT 2 cut(s) 511, 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.