MD04G1244000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013487
Physical Location & Seq
Reverse (-)
32037727 .. 32038789
1063 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1244000.v1.1.491

Sequence Viewer

Length: 504 bp
ATGATCCACCAAATCGAAATCAAAGGCCGCTCAAGAAAAAAGATCAAGGACCCACTTCCCAGCTCACACTCTTTTACCAAATTACCATCACCTTTTGCCACTCAAAGCTCACACCAAAGTAGCACCCATCTCAAGAAGCAATTCATGATGCCTGCTCGTGCTCATGGGGTCACCTTCTCTCTCCTCCTACTGCTGTCCTTTTTGGCTACTGCTATCTCCACCACTGAATCAGCAAGCTTAGACCTTGCCAATGGGTCTGCAGCTGTGGGGCCACTGGTGCCACTGATCGGAGCTGAAAAGATGAAGACTTTGGTGATGAATGAGACCAGGAGGAAGTTGGGGAGCTTTCAGATTTGTGCATTGTGTACTTGTTGTGGAGGTGCCAAAGGTCTTTGCTTGCCTTCTCCTTGTTGCTATGCCATCAATTGCAACATTCCAAACAGGCCTTTCGGTTTCTGTTCTTTCACACCTAAGACCTGTAATTGCTTTGGATGCCATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

17.98

Weight (kDa)

9.38

Isoelectric Point (pI)

59.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7866 PF25268 115 - 166 6e-23 Domain of unknown function (DUF7866)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65295
fragaria_vesca FvH4_6g00580
malus_domestica MD04G1244000.v1.1 MD12G1259800.v1.1
prunus_persica Prupe.1G131400_v2.0.a1 Prupe.6G360500_v2.0.a1
pyrus_communis pycom04g21490 pycom12g23790
rosa_chinensis RchiOBHm_Chr3g0447971
rosa_laevigata RLG00000025956
rosa_multiflora Rmu_sc0005969.1_g000009
rosa_roxburghii Rroxscaffold_6G00426160
rosa_rugosa Rorug02G0607500
rosa_samantha Rh3AG006700 Rh3BG006400 Rh3CG006600 Rh3DG007000
rosa_wichuraiana Rw3G000540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 277, 380
AccBSI CCGCTC 1 cut(s) 30
AciI CCGC 1 cut(s) 28
AfaI GTAC 1 cut(s) 367
AfiI CCNNNNNNNGG 1 cut(s) 287
AjnI CCWGG 1 cut(s) 326
AluBI AGCT 6 cut(s) 63, 108, 237, 263, 293, 345
AluI AGCT 6 cut(s) 63, 108, 237, 263, 293, 345
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 1 cut(s) 317
AoxI GGCC 3 cut(s) 25, 269, 443
ApeKI GCWGC 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 140
AspS9I GGNCC 2 cut(s) 49, 269
AsuHPI GGTGA 3 cut(s) 81, 163, 325
AvaII GGWCC 1 cut(s) 49
BanI GGYRCC 2 cut(s) 277, 380
BauI CACGAG 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 272
BccI CCATC 3 cut(s) 94, 135, 428
BciT130I CCWGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 258
BisI GCNGC 2 cut(s) 28, 261
BlsI GCNGC 2 cut(s) 29, 262
Bme1390I CCNGG 1 cut(s) 328
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 49, 269
BmiI GGNNCC 4 cut(s) 51, 270, 279, 382
BmrFI CCNGG 1 cut(s) 328
BmsI GCATC 2 cut(s) 138, 482
BpiI GAAGAC 1 cut(s) 311
BpuEI CTTGAG 2 cut(s) 16, 116
BsaI GGTCTC 1 cut(s) 317
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 328
BseGI GGATG 1 cut(s) 497
BseLI CCNNNNNNNGG 1 cut(s) 287
BseNI ACTGG 1 cut(s) 279
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 272
BseYI CCCAGC 1 cut(s) 59
BshFI GGCC 3 cut(s) 27, 271, 445
BshNI GGYRCC 2 cut(s) 277, 380
BsiHKAI GWGCWC 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 317
BsnI GGCC 3 cut(s) 27, 271, 445
Bso31I GGTCTC 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 3 cut(s) 3, 42, 285
BspACI CCGC 1 cut(s) 28
BspANI GGCC 3 cut(s) 27, 271, 445
BspHI TCATGA 1 cut(s) 144
BspLI GGNNCC 4 cut(s) 51, 270, 279, 382
BspMAI CTGCAG 1 cut(s) 262
BspT107I GGYRCC 2 cut(s) 277, 380
BspTNI GGTCTC 1 cut(s) 317
BsrBI CCGCTC 1 cut(s) 30
BsrI ACTGG 1 cut(s) 279
BssMI GATC 3 cut(s) 3, 42, 285
BssSI CACGAG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 328
BstC8I GCNNGC 3 cut(s) 153, 235, 398
BstDEI CTNAG 2 cut(s) 238, 471
BstEII GGTNACC 1 cut(s) 169
BstF5I GGATG 1 cut(s) 497
BstKTI GATC 3 cut(s) 6, 45, 288
BstMAI GTCTC 1 cut(s) 317
BstMBI GATC 3 cut(s) 3, 42, 285
BstMWI GCNNNNNNNGC 2 cut(s) 277, 492
BstNI CCWGG 1 cut(s) 328
BstPI GGTNACC 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 272
BstV2I GAAGAC 1 cut(s) 311
BsuRI GGCC 3 cut(s) 27, 271, 445
BtsCI GGATG 1 cut(s) 497
BtsIMutI CAGTG 3 cut(s) 222, 272, 281
Cac8I GCNNGC 3 cut(s) 153, 235, 398
CciI TCATGA 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 49, 269
Csp6I GTAC 1 cut(s) 366
CviAII CATG 2 cut(s) 145, 164
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 2 cut(s) 238, 471
DpnI GATC 3 cut(s) 5, 44, 287
DpnII GATC 3 cut(s) 3, 42, 285
Eco147I AGGCCT 1 cut(s) 445
Eco31I GGTCTC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 49
Eco91I GGTNACC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 49
EcoO65I GGTNACC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 326
FaeI CATG 2 cut(s) 148, 167
FaiI YATR 4 cut(s) 146, 165, 417, 498
FatI CATG 2 cut(s) 144, 163
Fnu4HI GCNGC 2 cut(s) 28, 261
Fsp4HI GCNGC 2 cut(s) 28, 261
GluI GCNGC 2 cut(s) 28, 261
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 3 cut(s) 27, 271, 445
Hin1II CATG 2 cut(s) 148, 167
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 1 cut(s) 227
HphI GGTGA 3 cut(s) 81, 163, 325
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 2 cut(s) 290, 351
Hpy188III TCNNGA 3 cut(s) 33, 133, 145
Hpy8I GTNNAC 1 cut(s) 366
HpyAV CCTTC 2 cut(s) 184, 411
HpyCH4V TGCA 3 cut(s) 260, 359, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 492
HpyF3I CTNAG 2 cut(s) 238, 471
Hsp92II CATG 2 cut(s) 148, 167
Kzo9I GATC 3 cut(s) 3, 42, 285
LmnI GCTCC 2 cut(s) 290, 342
LpnPI CCDG 7 cut(s) 73, 165, 260, 313, 340, 427, 490
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 2 cut(s) 138, 482
MaeIII GTNAC 1 cut(s) 169
MalI GATC 3 cut(s) 5, 44, 287
MbiI CCGCTC 1 cut(s) 30
MboI GATC 3 cut(s) 3, 42, 285
MboII GAAGA 1 cut(s) 316
MfeI CAATTG 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 4 cut(s) 80, 140, 424, 481
MnlI CCTC 3 cut(s) 194, 324, 371
MroXI GAANNNNTTC 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 328
MunI CAATTG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 328
MwoI GCNNNNNNNGC 2 cut(s) 277, 492
NdeII GATC 3 cut(s) 3, 42, 285
NlaIII CATG 2 cut(s) 148, 167
NlaIV GGNNCC 4 cut(s) 51, 270, 279, 382
NmuCI GTSAC 1 cut(s) 169
PagI TCATGA 1 cut(s) 144
PceI AGGCCT 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 29, 262
PpuMI RGGWCCY 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 326
PspEI GGTNACC 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 4 cut(s) 51, 270, 279, 382
PspPI GGNCC 2 cut(s) 49, 269
PspPPI RGGWCCY 1 cut(s) 49
PstI CTGCAG 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
SatI GCNGC 2 cut(s) 28, 261
Sau3AI GATC 3 cut(s) 3, 42, 285
Sau96I GGNCC 2 cut(s) 49, 269
ScrFI CCNGG 1 cut(s) 328
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 2 cut(s) 138, 482
SfcI CTRYAG 1 cut(s) 258
SinI GGWCC 1 cut(s) 49
SmlI CTYRAG 2 cut(s) 31, 131
SmoI CTYRAG 2 cut(s) 31, 131
Sse9I AATT 4 cut(s) 80, 140, 424, 481
SseBI AGGCCT 1 cut(s) 445
SsiI CCGC 1 cut(s) 28
StuI AGGCCT 1 cut(s) 445
StyD4I CCNGG 1 cut(s) 326
TaqI TCGA 1 cut(s) 15
TasI AATT 4 cut(s) 80, 140, 424, 481
TatI WGTACW 1 cut(s) 365
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 1 cut(s) 227
TscAI CASTG 3 cut(s) 229, 279, 288
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 3 cut(s) 133, 317, 332
TspRI CASTG 3 cut(s) 229, 279, 288
VpaK11BI GGWCC 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.