MD05G1001800.v1.1

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003815
Physical Location & Seq
Reverse (-)
869307 .. 874614
5308 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1001800.v1.1.491

Sequence Viewer

Length: 1854 bp
ATGCAGAGATCACGTGGCGCTCTTCTGAACAGAAGATCTTTAGCGATCAGCGGAAAGAACCACTTGTATAAGGTGTCTCTGTCTTTCGTATTTGTCCTCTGGGGACTTGTCTTTCTATTCAGCTTATGGTTCAGTTGTGGACATGCATACAAAGATGGATCTACTGTATCTCCAGTTGGTATATCAACTTGGGACGAAGCTAAGCTGGACCGTGATGAACACTATGATTCTGTAAATATACAAAAAGAAACTGATTTGGTCTACTCTTCTGGAGGTGAATGCACAAATGGGATTGAAACATGTGGTTTAAAGGGTGAGTTATCTGCTACAGAAAGAAGTCAACACCATGTCTCAGCTGAAGGAAGTAGACAGCATGCCTCAGCTGAAGGAAGTCTACATCATGCCTCAGCTGAAGGAAGTATATTTCATGACTCAGCTGTTGGTGAGCAACCTGAGGTGGTGAATGCTGGTTCAGGTGCAAAACTTGAAAATGATGCATTAAAGAATGGTCGCTTGCCACGTGCTGTGCCTCTTGGTCTTGATGAATTCAAAAGCAAAACATTTAGTTCCAAAAGTAAATCTGGAAATGGTCAGGCTGAAGGCATAAAACATAGGGTGGAGCCTGGTGGTGCAGAGTACAATTATGCGTCAGCCGCAAAGGGAGCCAAGGTCTTGGCTTTTAACAAGGAAGCTAAGGGTGCCTCTAATATCTTAGGCAAAGACAAAGACAAGTACCTTCGCAATCCATGTTCTGCAGAAGAAAAGTTTGTTGATATAGAGCTTTCCGAAGAAACCTTAGTAGATACAATTGAAATAGCTAATCTTGAGCACTATTCATCTAATTTAAAAGATTTTGAGGTGCTCGGCAGTTTGGCTTATCCAACTAATGAATGGGTCTTTCTTGGGAATGGTACTGCTGCAAATAATAAACTTGTACAAAGGTTTGTTCTTCAGCAACCCAAGTGGGTGAGATATATTAAGTTGAAACTGTTGAGCCATTATGGTTCAGAATTCTACTGCACGCTTAGTATTATTGAACTCTATGGAGTGGATGCTGTTGAGCGAATGCTTGAGGATTTGATTTCTGTTGAAGGCGGCTCGTTTGTTACTTCGGGAGCAGCAGTTGACCAGAAACCTGTGCCCTCCCACCCGGACTCCCCTGAGGGTGAGGAATTGTATCCTGATATGGTTAAGGAATTGGAACCCCAAGATGCAGCTGGAGGGTCTGAAGTGAATAATAATACGATGAATAGTGAAGTGCGTGATCCTGTCAAAGAAGTTCGTCATCAACAAGTTAATAGGATGCCTGGGGACACTGTTCTAAAAATTTTAATGCAGAAAGTTCGTTCATTAGATTATAGTTTATCAGTTCTGGAGCGATACCTGGAGGAATCAACATCAAAATATGGCAGTATTTTCAGAGAATTTGACAAAGATTTGGGAGAGAAAGATATAGATTTAAAGAAGATCAGAGAAGACATAAGGAATCTTGTTCTCAGCCAGGAAGTCATTGCTAAAGATGTTGACAATCTTATATCTTGGCAGTCCCTAGTTACCATGCAATTGGATAATCTAGTCAGGGACAATGCTATCCTCAGATGTGAGGTTGAAAGGGTGAGGGAGAAGCAGGCATCTGTAGATAATAAGGGTATCATAATATTTCTTATATGCATAATTTTTTCATTGTTAGCTCTTGTGAGGCTTTTCACAGAAATGGCAGTTAGCGTTTGTATGATGGCATCGAGTGTTGATCCAACGACAGAAAAGTCGAGGAAGTTTTGTTGGACGAGTTCTTCGTCCTGGATTTTTCTACTTGCGAGCTGTATCCTTGTCCTATTTATATTATCATTATAA

Protein Analysis

618

Amino Acids

68.45

Weight (kDa)

5.44

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 228 - 350 2.5e-32 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1852
AasI GACNNNNNNGTC 1 cut(s) 1765
AccB1I GGYRCC 1 cut(s) 698
AccI GTMKAC 3 cut(s) 261, 367, 394
AciI CCGC 3 cut(s) 51, 654, 1095
AclWI GGATC 3 cut(s) 166, 1259, 1745
AcsI RAATTY 4 cut(s) 545, 1010, 1326, 1424
AcuI CTGAAG 6 cut(s) 378, 405, 432, 618, 935, 1248
AcvI CACGTG 2 cut(s) 14, 521
AfaI GTAC 4 cut(s) 638, 734, 913, 936
AflIII ACRYGT 1 cut(s) 299
AgsI TTSAA 8 cut(s) 296, 488, 550, 812, 985, 1037, 1091, 1610
AjnI CCWGG 5 cut(s) 622, 1306, 1383, 1500, 1799
Alw21I GWGCWC 2 cut(s) 831, 864
Alw26I GTCTC 2 cut(s) 81, 355
AlwI GGATC 3 cut(s) 166, 1259, 1745
ApeKI GCWGC 3 cut(s) 917, 1118, 1214
ApoI RAATTY 4 cut(s) 545, 1010, 1326, 1424
ArsI GACNNNNNNTTYG 2 cut(s) 1777, 1809
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 1151
AsuHPI GGTGA 7 cut(s) 287, 326, 455, 472, 979, 1178, 1627
AvaII GGWCC 1 cut(s) 208
AxyI CCTNAGG 2 cut(s) 453, 1161
BaeGI GKGCMC 1 cut(s) 1143
BanI GGYRCC 1 cut(s) 698
BarI GAAGNNNNNNTAC 2 cut(s) 378, 410
BbrPI CACGTG 2 cut(s) 14, 521
BbsI GAAGAC 1 cut(s) 1482
Bbv12I GWGCWC 2 cut(s) 831, 864
BbvCI CCTCAGC 2 cut(s) 379, 406
BbvI GCAGC 3 cut(s) 904, 1130, 1226
BccI CCATC 2 cut(s) 149, 1729
BcgI CGANNNNNNTGC 2 cut(s) 1325, 1359
BciT130I CCWGG 5 cut(s) 624, 1308, 1385, 1502, 1801
BciVI GTATCC 2 cut(s) 1188, 1835
BcnI CCSGG 1 cut(s) 1151
BcoDI GTCTC 2 cut(s) 81, 355
BfaI CTAG 2 cut(s) 1550, 1574
BfmI CTRYAG 3 cut(s) 327, 753, 1635
BfoI RGCGCY 1 cut(s) 21
BfuI GTATCC 2 cut(s) 1188, 1835
BglII AGATCT 1 cut(s) 35
BisI GCNGC 5 cut(s) 654, 918, 1096, 1119, 1215
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 5 cut(s) 655, 919, 1097, 1120, 1216
Bme1390I CCNGG 6 cut(s) 624, 1151, 1308, 1385, 1502, 1801
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 4 cut(s) 621, 664, 700, 1203
BmrFI CCNGG 6 cut(s) 624, 1151, 1308, 1385, 1502, 1801
BmsI GCATC 6 cut(s) 484, 1042, 1201, 1293, 1640, 1748
BpiI GAAGAC 1 cut(s) 1482
BpmI CTGGAG 5 cut(s) 156, 291, 1239, 1394, 1406
Bpu10I CCTNAGC 3 cut(s) 379, 406, 693
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 845, 1091
BpuMI CCSGG 1 cut(s) 1151
BsaAI YACGTR 2 cut(s) 14, 521
BsaJI CCNNGG 2 cut(s) 666, 1307
BsaXI ACNNNNNCTCC 8 cut(s) 154, 184, 654, 684, 1107, 1137, 1139, 1169
Bse1I ACTGG 1 cut(s) 173
Bse21I CCTNAGG 2 cut(s) 453, 1161
Bse3DI GCAATG 1 cut(s) 1509
BseBI CCWGG 5 cut(s) 624, 1308, 1385, 1502, 1801
BseDI CCNNGG 2 cut(s) 666, 1307
BseGI GGATG 2 cut(s) 1057, 1308
BseMI GCAATG 1 cut(s) 1509
BseMII CTCAG 8 cut(s) 366, 393, 420, 444, 447, 1152, 1510, 1609
BseNI ACTGG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 1143
BseXI GCAGC 3 cut(s) 904, 1130, 1226
BsgI GTGCAG 2 cut(s) 651, 1003
BshNI GGYRCC 1 cut(s) 698
BsiHKAI GWGCWC 2 cut(s) 831, 864
BsiSI CCGG 1 cut(s) 1151
BslFI GGGAC 5 cut(s) 117, 206, 1325, 1531, 1595
BsmAI GTCTC 2 cut(s) 81, 355
BsmFI GGGAC 5 cut(s) 117, 206, 1325, 1531, 1595
BsmI GAATGC 3 cut(s) 284, 469, 1071
Bsp1286I GDGCHC 3 cut(s) 831, 864, 1143
Bsp1407I TGTACA 1 cut(s) 934
Bsp143I GATC 7 cut(s) 8, 35, 45, 158, 1264, 1467, 1750
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 3 cut(s) 51, 654, 1095
BspCNI CTCAG 8 cut(s) 365, 392, 419, 445, 446, 1153, 1509, 1608
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 4 cut(s) 621, 664, 700, 1203
BspMAI CTGCAG 1 cut(s) 757
BspPI GGATC 3 cut(s) 166, 1259, 1745
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 1509
BsrGI TGTACA 1 cut(s) 934
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 666, 1307
BssMI GATC 7 cut(s) 8, 35, 45, 158, 1264, 1467, 1750
BssT1I CCWWGG 1 cut(s) 666
Bst2UI CCWGG 5 cut(s) 624, 1308, 1385, 1502, 1801
Bst4CI ACNGT 4 cut(s) 166, 212, 990, 1318
Bst6I CTCTTC 2 cut(s) 27, 271
BstAUI TGTACA 1 cut(s) 934
BstBAI YACGTR 2 cut(s) 14, 521
BstC8I GCNNGC 5 cut(s) 375, 515, 1022, 1629, 1819
BstF5I GGATG 2 cut(s) 1057, 1308
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 7 cut(s) 11, 38, 48, 161, 1267, 1470, 1753
BstMAI GTCTC 2 cut(s) 81, 355
BstMBI GATC 7 cut(s) 8, 35, 45, 158, 1264, 1467, 1750
BstMWI GCNNNNNNNGC 3 cut(s) 653, 662, 698
BstNI CCWGG 5 cut(s) 624, 1308, 1385, 1502, 1801
BstNSI RCATGY 3 cut(s) 146, 303, 377
BstSCI CCNGG 6 cut(s) 622, 1149, 1306, 1383, 1500, 1799
BstSFI CTRYAG 3 cut(s) 327, 753, 1635
BstSLI GKGCMC 1 cut(s) 1143
BstV1I GCAGC 3 cut(s) 904, 1130, 1226
BstV2I GAAGAC 1 cut(s) 1482
BstX2I RGATCY 2 cut(s) 35, 158
BstXI CCANNNNNNTGG 2 cut(s) 673, 1564
BstYI RGATCY 2 cut(s) 35, 158
Bsu36I CCTNAGG 2 cut(s) 453, 1161
BsuI GTATCC 2 cut(s) 1188, 1835
BtsCI GGATG 2 cut(s) 1057, 1308
BtsIMutI CAGTG 1 cut(s) 1314
Cac8I GCNNGC 5 cut(s) 375, 515, 1022, 1629, 1819
CciI TCATGA 1 cut(s) 427
CfoI GCGC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 1 cut(s) 636
Csp6I GTAC 4 cut(s) 637, 733, 912, 935
CspCI CAANNNNNGTGG 2 cut(s) 944, 979
CviAII CATG 8 cut(s) 143, 300, 347, 374, 401, 428, 747, 1558
CviQI GTAC 4 cut(s) 637, 733, 912, 935
DpnI GATC 7 cut(s) 10, 37, 47, 160, 1266, 1469, 1752
DpnII GATC 7 cut(s) 8, 35, 45, 158, 1264, 1467, 1750
DraI TTTAAA 3 cut(s) 309, 846, 1461
DrdI GACNNNNNNGTC 1 cut(s) 1765
DseDI GACNNNNNNGTC 1 cut(s) 1765
Eam1104I CTCTTC 2 cut(s) 27, 271
EarI CTCTTC 2 cut(s) 27, 271
Eco130I CCWWGG 1 cut(s) 666
Eco47I GGWCC 1 cut(s) 208
Eco57I CTGAAG 6 cut(s) 378, 405, 432, 618, 935, 1248
Eco72I CACGTG 2 cut(s) 14, 521
Eco81I CCTNAGG 2 cut(s) 453, 1161
EcoRI GAATTC 2 cut(s) 545, 1010
EcoRII CCWGG 5 cut(s) 622, 1306, 1383, 1500, 1799
EcoT14I CCWWGG 1 cut(s) 666
EcoT22I ATGCAT 3 cut(s) 148, 499, 1673
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 8 cut(s) 146, 303, 350, 377, 404, 431, 750, 1561
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FaqI GGGAC 5 cut(s) 117, 206, 1325, 1531, 1595
FatI CATG 8 cut(s) 142, 299, 346, 373, 400, 427, 746, 1557
FblI GTMKAC 3 cut(s) 261, 367, 394
Fnu4HI GCNGC 5 cut(s) 654, 918, 1096, 1119, 1215
FokI GGATG 2 cut(s) 1064, 1315
Fsp4HI GCNGC 5 cut(s) 654, 918, 1096, 1119, 1215
FspBI CTAG 2 cut(s) 1550, 1574
GlaI GCGC 1 cut(s) 19
GluI GCNGC 5 cut(s) 654, 918, 1096, 1119, 1215
GsuI CTGGAG 5 cut(s) 156, 291, 1239, 1394, 1406
HaeII RGCGCY 1 cut(s) 21
HapII CCGG 1 cut(s) 1151
HgaI GACGC 1 cut(s) 636
HhaI GCGC 1 cut(s) 20
Hin1II CATG 8 cut(s) 146, 303, 350, 377, 404, 431, 750, 1561
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HincII GTYRAC 3 cut(s) 341, 1126, 1525
HindII GTYRAC 3 cut(s) 341, 1126, 1525
HinfI GANTC 5 cut(s) 227, 431, 1154, 1391, 1486
HpaII CCGG 1 cut(s) 1151
HphI GGTGA 7 cut(s) 287, 326, 455, 472, 979, 1178, 1627
Hpy166II GTNNAC 7 cut(s) 140, 262, 341, 368, 395, 1126, 1525
Hpy188I TCNGA 7 cut(s) 27, 787, 1009, 1228, 1421, 1472, 1598
Hpy188III TCNNGA 8 cut(s) 270, 428, 539, 582, 824, 1113, 1181, 1373
Hpy8I GTNNAC 7 cut(s) 140, 262, 341, 368, 395, 1126, 1525
HpyAV CCTTC 6 cut(s) 353, 380, 407, 593, 746, 1085
HpyCH4III ACNGT 4 cut(s) 166, 212, 990, 1318
HpyCH4IV ACGT 2 cut(s) 13, 520
HpyF10VI GCNNNNNNNGC 3 cut(s) 653, 662, 698
HpySE526I ACGT 2 cut(s) 13, 520
Hsp92II CATG 8 cut(s) 146, 303, 350, 377, 404, 431, 750, 1561
HspAI GCGC 1 cut(s) 18
Kzo9I GATC 7 cut(s) 8, 35, 45, 158, 1264, 1467, 1750
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 4 cut(s) 619, 662, 1115, 1375
Lsp1109I GCAGC 3 cut(s) 904, 1130, 1226
LweI GCATC 6 cut(s) 484, 1042, 1201, 1293, 1640, 1748
MaeI CTAG 2 cut(s) 1550, 1574
MaeII ACGT 2 cut(s) 13, 520
MaeIII GTNAC 2 cut(s) 1105, 1552
MalI GATC 7 cut(s) 10, 37, 47, 160, 1266, 1469, 1752
MboI GATC 7 cut(s) 8, 35, 45, 158, 1264, 1467, 1750
MboII GAAGA 9 cut(s) 14, 45, 258, 770, 800, 941, 1477, 1487, 1785
MfeI CAATTG 2 cut(s) 807, 1562
MflI RGATCY 2 cut(s) 35, 158
MhlI GDGCHC 3 cut(s) 831, 864, 1143
MlyI GAGTC 2 cut(s) 425, 1148
MmeI TCCRAC 3 cut(s) 905, 1763, 1778
Mph1103I ATGCAT 3 cut(s) 148, 499, 1673
MseI TTAA 9 cut(s) 308, 500, 681, 845, 978, 1191, 1296, 1331, 1460
MslI CAYNNNNRTG 1 cut(s) 1712
MspA1I CMGCKG 6 cut(s) 51, 356, 383, 410, 437, 1217
MspI CCGG 1 cut(s) 1151
MspR9I CCNGG 6 cut(s) 624, 1151, 1308, 1385, 1502, 1801
MunI CAATTG 2 cut(s) 807, 1562
Mva1269I GAATGC 3 cut(s) 284, 469, 1071
MvaI CCWGG 5 cut(s) 624, 1308, 1385, 1502, 1801
MwoI GCNNNNNNNGC 3 cut(s) 653, 662, 698
NciI CCSGG 1 cut(s) 1151
NdeII GATC 7 cut(s) 8, 35, 45, 158, 1264, 1467, 1750
NlaIII CATG 8 cut(s) 146, 303, 350, 377, 404, 431, 750, 1561
NlaIV GGNNCC 4 cut(s) 621, 664, 700, 1203
NmeAIII GCCGAG 1 cut(s) 843
NsiI ATGCAT 3 cut(s) 148, 499, 1673
NspI RCATGY 3 cut(s) 146, 303, 377
PaeI GCATGC 1 cut(s) 377
PagI TCATGA 1 cut(s) 427
PciI ACATGT 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 2 cut(s) 517, 1793
PctI GAATGC 3 cut(s) 284, 469, 1071
PfeI GAWTC 3 cut(s) 227, 1391, 1486
PfoI TCCNGGA 1 cut(s) 1799
PkrI GCNGC 5 cut(s) 655, 919, 1097, 1120, 1216
PleI GAGTC 2 cut(s) 425, 1148
PmaCI CACGTG 2 cut(s) 14, 521
PmlI CACGTG 2 cut(s) 14, 521
PpsI GAGTC 2 cut(s) 425, 1148
Ppu21I YACGTR 2 cut(s) 14, 521
PscI ACATGT 1 cut(s) 299
PsiI TTATAA 1 cut(s) 1852
Psp6I CCWGG 5 cut(s) 622, 1306, 1383, 1500, 1799
PspCI CACGTG 2 cut(s) 14, 521
PspGI CCWGG 5 cut(s) 622, 1306, 1383, 1500, 1799
PspN4I GGNNCC 4 cut(s) 621, 664, 700, 1203
PspPI GGNCC 1 cut(s) 208
PstI CTGCAG 1 cut(s) 757
PsuI RGATCY 2 cut(s) 35, 158
PvuII CAGCTG 5 cut(s) 356, 383, 410, 437, 1217
RsaI GTAC 4 cut(s) 638, 734, 913, 936
RsaNI GTAC 4 cut(s) 637, 733, 912, 935
RseI CAYNNNNRTG 1 cut(s) 1712
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 9 cut(s) 308, 500, 681, 845, 978, 1191, 1296, 1331, 1460
SatI GCNGC 5 cut(s) 654, 918, 1096, 1119, 1215
Sau3AI GATC 7 cut(s) 8, 35, 45, 158, 1264, 1467, 1750
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 2 cut(s) 425, 1148
ScrFI CCNGG 6 cut(s) 624, 1151, 1308, 1385, 1502, 1801
SduI GDGCHC 3 cut(s) 831, 864, 1143
SfaNI GCATC 6 cut(s) 484, 1042, 1201, 1293, 1640, 1748
SfcI CTRYAG 3 cut(s) 327, 753, 1635
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 1712
SmlI CTYRAG 2 cut(s) 824, 1070
SmoI CTYRAG 2 cut(s) 824, 1070
SphI GCATGC 1 cut(s) 377
SsiI CCGC 3 cut(s) 51, 654, 1095
SspI AATATT 1 cut(s) 1659
SspMI CTAG 2 cut(s) 1550, 1574
StyD4I CCNGG 6 cut(s) 622, 1149, 1306, 1383, 1500, 1799
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 4 cut(s) 166, 212, 990, 1318
TaiI ACGT 2 cut(s) 16, 523
TaqI TCGA 2 cut(s) 1742, 1769
TatI WGTACW 2 cut(s) 636, 934
TauI GCSGC 2 cut(s) 656, 1098
TfiI GAWTC 3 cut(s) 227, 1391, 1486
Tru1I TTAA 9 cut(s) 308, 500, 681, 845, 978, 1191, 1296, 1331, 1460
Tru9I TTAA 9 cut(s) 308, 500, 681, 845, 978, 1191, 1296, 1331, 1460
TscAI CASTG 1 cut(s) 1321
TseI GCWGC 3 cut(s) 917, 1118, 1214
TspDTI ATGAA 8 cut(s) 231, 416, 558, 825, 903, 1262, 1338, 1671
TspRI CASTG 1 cut(s) 1321
VpaK11BI GGWCC 1 cut(s) 208
XapI RAATTY 4 cut(s) 545, 1010, 1326, 1424
XceI RCATGY 3 cut(s) 146, 303, 377
XcmI CCANNNNNNNNNTGG 2 cut(s) 888, 1214
XmiI GTMKAC 3 cut(s) 261, 367, 394
XspI CTAG 2 cut(s) 1550, 1574
Zsp2I ATGCAT 3 cut(s) 148, 499, 1673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.