Rh6DG004200

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003815
Physical Location & Seq
Reverse (-)
500839 .. 506055
5217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG004200.1

Sequence Viewer

Length: 1689 bp
ATGCAGAGGTCGCGTAGAGCTCTTCTGCATAGAAGAGCTTTGGAAAAGGTCATCACCGGAAGGAGCCGCTTGTATAAGGTGTCTCTATCTTTAGTTTTTGCTCTGTGGGGGTTTGTCTTCCTTATCAGCTTATGGTTTAGTCATGGCGACGGACACAGAGATGGATCCACAGCATCTCCAGTTGGATTATCAACTTGGAATGAAACTAAGCTGGATTGTGATGAACACACTGATTCTGTAGAACTACAAAAGCAAGAAGATTTAGTCTACTCTTCTGAAGGTGTGTGCACAAATGGTGTTGAAACTAGTAGTTTAAATGGTGAACTACTTAGTGAAGGAAATAGACATCAGGCCTCAACAGAAGGAAGTGCAATTTATAACTCGGCCGTAGCTGAGCAACCTGAATTGGAGAAATCTAGTTCAGGCGTGAAACATGAAATTGATGCTCCAAAAAATGGCCGCCTGCCTCGTTCTGTGCCTCTTGGTCTGGATGAATTCAAGAGCAAAACATTTAGTTCAAAAATTAAATCTGTAATTGGTCAGGCTGGAGGCATAAAACATAGAGTGGAGCCTGGTGGTACAGAGTACAATTACGCTTCAGCTGCAAAGGGAGCCAAGGTCTTGGCTTTTAACAAGGAAGCCAAGGGTGCCTCTAATATCATAAGCAGAGACAAGGACAAGTACCTTCGCAATCCATGTTCTGCAGAAGAAAAGTTTGTTGATATAGAACTTTCAGAAGAAACCTTGGTAGACACAATCAAAATAGCAAATATTAAGCTTGCGCAAAGATTTGATCTTGAGGAACCTAAGTGGGTGAGATATATAAGGTTAAAGCTTTTAAACCATCATGGTTCAGAGTTCTATTGCACAGTCAGTGTTATTGAAATTTATGGAATTGATGCTGTTGAGCGAATGCTTGAGGATTTAATTTCTGTTGAAAGCGGCGCATATGTTTCTAATGGAGTAACTGTTGACCAGAAACCTGTGACCTCCCACCCAGATTCCCCTGAGGGTGATGATTTTTTTAATATTGATAAAGAAATGGAACCTCAAGCTGCAGTTGAAAACTATAATGTGAACAATGAAATAATCAAGAGTGATGTGCCTGATCCTATCAAAGAAGTACTTCATCAACAAGGTAGCAGGATGCCTGGGGATACTGTTCTTAAGATACTAATGCAGAAAGTCCGTTCATTGGATTTTAGTTTATCACTACTTGAGCGATACCTTGAGGAATCAAATTCAAGATACGGCAGCATTTTCAAAGAATTCGAGACAGATATGGAAGAGAAAGAGTTAGATTTACAGAAGATCAAAGAAAACATGAGGAATCTCTTAGAAAGCCAGGAAGTAATTGCTAAAGATGTAAACAATCTCATGTCTTGGCAGTCCCTTGTCTCTGTGCAATTGGATAATTTAGTCAGGGATAACGCTATCCTCAGGTATGAGGTTGAAAAGGTCAGGGAGAAGCAGATATCTGTAGATAATAAGGGTGTTGTAATATTTGTAGTCTGTGTACTTTTCTCATTGTTGGCCCTTGCAAGGCTTTTTATAGATATTGTGGGTAGTGTTTATAGTGCATTCAGTGTTCATACAACAGAGAAATCCAGGAAATTTTGTTTGATGAGCTCTTCCTGGGTTTCCTTACTAGTGAGCTGTATCCTTGTCCTATTTATATTATCTCTATAA

Protein Analysis

562

Amino Acids

63.08

Weight (kDa)

5.45

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 261 - 298 1.3e-08 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
Acc16I TGCGCA 1 cut(s) 783
AccB1I GGYRCC 1 cut(s) 647
AccB7I CCANNNNNTGG 1 cut(s) 455
AccI GTMKAC 2 cut(s) 267, 750
AccII CGCG 1 cut(s) 13
AciI CCGC 3 cut(s) 67, 460, 942
AclWI GGATC 3 cut(s) 159, 172, 1103
AcoI YGGCCR 2 cut(s) 384, 457
AcsI RAATTY 5 cut(s) 494, 885, 1240, 1268, 1613
AcuI CTGAAG 2 cut(s) 297, 582
AfaI GTAC 5 cut(s) 580, 587, 683, 1125, 1518
AfiI CCNNNNNNNGG 3 cut(s) 455, 1195, 1542
AflII CTTAAG 1 cut(s) 1166
AgsI TTSAA 9 cut(s) 302, 499, 519, 884, 938, 1064, 1245, 1264, 1454
AhlI ACTAGT 2 cut(s) 305, 1648
AjnI CCWGG 5 cut(s) 571, 1150, 1344, 1607, 1634
Alw21I GWGCWC 3 cut(s) 22, 290, 1631
Alw26I GTCTC 4 cut(s) 87, 663, 1268, 1402
Alw44I GTGCAC 1 cut(s) 286
AlwI GGATC 3 cut(s) 159, 172, 1103
AoxI GGCC 4 cut(s) 351, 384, 457, 1533
ApaLI GTGCAC 1 cut(s) 286
ApeKI GCWGC 3 cut(s) 602, 1055, 1254
ApoI RAATTY 5 cut(s) 494, 885, 1240, 1268, 1613
Asp700I GAANNNNTTC 1 cut(s) 1125
AspLEI GCGC 2 cut(s) 784, 947
AspS9I GGNCC 1 cut(s) 1534
AsuHPI GGTGA 4 cut(s) 46, 332, 826, 1025
AxyI CCTNAGG 2 cut(s) 1008, 1439
BaeGI GKGCMC 1 cut(s) 290
BamHI GGATCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 2 cut(s) 22, 1631
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 3 cut(s) 22, 290, 1631
BbvI GCAGC 3 cut(s) 589, 1042, 1266
BccI CCATC 2 cut(s) 155, 852
BceAI ACGGC 2 cut(s) 371, 1267
BciT130I CCWGG 5 cut(s) 573, 1152, 1346, 1609, 1636
BciVI GTATCC 2 cut(s) 1150, 1670
BcoDI GTCTC 4 cut(s) 87, 663, 1268, 1402
BcuI ACTAGT 2 cut(s) 305, 1648
BfaI CTAG 3 cut(s) 306, 417, 1649
BfmI CTRYAG 4 cut(s) 237, 702, 1056, 1479
BfrI CTTAAG 1 cut(s) 1166
BfuI GTATCC 2 cut(s) 1150, 1670
BisI GCNGC 6 cut(s) 67, 460, 603, 943, 1056, 1255
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 6 cut(s) 68, 461, 604, 944, 1057, 1256
BmcAI AGTACT 1 cut(s) 1125
Bme1390I CCNGG 5 cut(s) 573, 1152, 1346, 1609, 1636
BmgT120I GGNCC 1 cut(s) 1534
BmiI GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 804, 1047
BmrFI CCNGG 5 cut(s) 573, 1152, 1346, 1609, 1636
BmsI GCATC 4 cut(s) 182, 433, 889, 1137
BpiI GAAGAC 1 cut(s) 109
BpmI CTGGAG 2 cut(s) 162, 567
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 5 cut(s) 818, 938, 1035, 1238, 1250
BsaJI CCNNGG 5 cut(s) 615, 642, 744, 1151, 1635
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 6 cut(s) 160, 190, 603, 633, 954, 984
Bsc4I CCNNNNNNNGG 3 cut(s) 455, 1195, 1542
Bse1I ACTGG 1 cut(s) 179
Bse21I CCTNAGG 2 cut(s) 1008, 1439
BseBI CCWGG 5 cut(s) 573, 1152, 1346, 1609, 1636
BseDI CCNNGG 5 cut(s) 615, 642, 744, 1151, 1635
BseGI GGATG 2 cut(s) 496, 1152
BseLI CCNNNNNNNGG 3 cut(s) 455, 1195, 1542
BseMII CTCAG 3 cut(s) 384, 999, 1453
BseNI ACTGG 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 290
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 3 cut(s) 589, 1042, 1266
Bsh1236I CGCG 1 cut(s) 13
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 4 cut(s) 353, 386, 459, 1535
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 3 cut(s) 22, 290, 1631
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 1 cut(s) 1375
BslI CCNNNNNNNGG 3 cut(s) 455, 1195, 1542
BsmAI GTCTC 4 cut(s) 87, 663, 1268, 1402
BsmFI GGGAC 1 cut(s) 1375
BsmI GAATGC 2 cut(s) 918, 1580
BsnI GGCC 4 cut(s) 353, 386, 459, 1535
Bsp1286I GDGCHC 3 cut(s) 22, 290, 1631
Bsp143I GATC 4 cut(s) 164, 793, 1108, 1311
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 3 cut(s) 67, 460, 942
BspANI GGCC 4 cut(s) 353, 386, 459, 1535
BspCNI CTCAG 3 cut(s) 385, 1000, 1452
BspFNI CGCG 1 cut(s) 13
BspLI GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 804, 1047
BspMAI CTGCAG 2 cut(s) 706, 1060
BspPI GGATC 3 cut(s) 159, 172, 1103
BspQI GCTCTTC 3 cut(s) 27, 28, 1636
BspT107I GGYRCC 1 cut(s) 647
BspTI CTTAAG 1 cut(s) 1166
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 5 cut(s) 615, 642, 744, 1151, 1635
BssMI GATC 4 cut(s) 164, 793, 1108, 1311
BssT1I CCWWGG 3 cut(s) 615, 642, 744
Bst2UI CCWGG 5 cut(s) 573, 1152, 1346, 1609, 1636
Bst4CI ACNGT 3 cut(s) 871, 970, 1162
Bst6I CTCTTC 5 cut(s) 27, 28, 277, 1281, 1636
BstAFI CTTAAG 1 cut(s) 1166
BstC8I GCNNGC 2 cut(s) 464, 780
BstDEI CTNAG 7 cut(s) 207, 329, 393, 807, 1008, 1336, 1439
BstF5I GGATG 2 cut(s) 496, 1152
BstFNI CGCG 1 cut(s) 13
BstHHI GCGC 2 cut(s) 784, 947
BstKTI GATC 4 cut(s) 167, 796, 1111, 1314
BstMAI GTCTC 4 cut(s) 87, 663, 1268, 1402
BstMBI GATC 4 cut(s) 164, 793, 1108, 1311
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
BstNI CCWGG 5 cut(s) 573, 1152, 1346, 1609, 1636
BstSCI CCNGG 5 cut(s) 571, 1150, 1344, 1607, 1634
BstSFI CTRYAG 4 cut(s) 237, 702, 1056, 1479
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 3 cut(s) 589, 1042, 1266
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 164
BstXI CCANNNNNNTGG 1 cut(s) 622
BstYI RGATCY 1 cut(s) 164
BstZI CGGCCG 1 cut(s) 384
Bsu36I CCTNAGG 2 cut(s) 1008, 1439
BsuI GTATCC 2 cut(s) 1150, 1670
BsuRI GGCC 4 cut(s) 353, 386, 459, 1535
BtsCI GGATG 2 cut(s) 496, 1152
BtsIMutI CAGTG 3 cut(s) 228, 880, 1591
Cac8I GCNNGC 2 cut(s) 464, 780
CfoI GCGC 2 cut(s) 784, 947
Cfr13I GGNCC 1 cut(s) 1534
Csp6I GTAC 5 cut(s) 579, 586, 682, 1124, 1517
CviAII CATG 6 cut(s) 143, 434, 696, 848, 1324, 1378
CviQI GTAC 5 cut(s) 579, 586, 682, 1124, 1517
DdeI CTNAG 7 cut(s) 207, 329, 393, 807, 1008, 1336, 1439
DpnI GATC 4 cut(s) 166, 795, 1110, 1313
DpnII GATC 4 cut(s) 164, 793, 1108, 1311
DraI TTTAAA 2 cut(s) 315, 840
EaeI YGGCCR 2 cut(s) 384, 457
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 5 cut(s) 27, 28, 277, 1281, 1636
EarI CTCTTC 5 cut(s) 27, 28, 277, 1281, 1636
Ecl136II GAGCTC 2 cut(s) 20, 1629
EclXI CGGCCG 1 cut(s) 384
Eco130I CCWWGG 3 cut(s) 615, 642, 744
Eco147I AGGCCT 1 cut(s) 353
Eco24I GRGCYC 2 cut(s) 22, 1631
Eco32I GATATC 1 cut(s) 1476
Eco52I CGGCCG 1 cut(s) 384
Eco53kI GAGCTC 2 cut(s) 20, 1629
Eco57I CTGAAG 2 cut(s) 297, 582
Eco81I CCTNAGG 2 cut(s) 1008, 1439
EcoICRI GAGCTC 2 cut(s) 20, 1629
EcoRI GAATTC 2 cut(s) 494, 1268
EcoRII CCWGG 5 cut(s) 571, 1150, 1344, 1607, 1634
EcoRV GATATC 1 cut(s) 1476
EcoT14I CCWWGG 3 cut(s) 615, 642, 744
EcoT38I GRGCYC 2 cut(s) 22, 1631
ErhI CCWWGG 3 cut(s) 615, 642, 744
FaeI CATG 6 cut(s) 146, 437, 699, 851, 1327, 1381
FalI AAGNNNNNCTT 2 cut(s) 1110, 1142
FaqI GGGAC 1 cut(s) 1375
FatI CATG 6 cut(s) 142, 433, 695, 847, 1323, 1377
FauNDI CATATG 1 cut(s) 949
FblI GTMKAC 2 cut(s) 267, 750
Fnu4HI GCNGC 6 cut(s) 67, 460, 603, 943, 1056, 1255
FokI GGATG 2 cut(s) 503, 1159
FriOI GRGCYC 2 cut(s) 22, 1631
Fsp4HI GCNGC 6 cut(s) 67, 460, 603, 943, 1056, 1255
FspBI CTAG 3 cut(s) 306, 417, 1649
FspI TGCGCA 1 cut(s) 783
GlaI GCGC 2 cut(s) 783, 946
GluI GCNGC 6 cut(s) 67, 460, 603, 943, 1056, 1255
GsuI CTGGAG 2 cut(s) 162, 567
HaeIII GGCC 4 cut(s) 353, 386, 459, 1535
HapII CCGG 1 cut(s) 57
HhaI GCGC 2 cut(s) 784, 947
Hin1II CATG 6 cut(s) 146, 437, 699, 851, 1327, 1381
Hin6I GCGC 2 cut(s) 782, 945
HinP1I GCGC 2 cut(s) 782, 945
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HindIII AAGCTT 2 cut(s) 776, 833
HinfI GANTC 4 cut(s) 233, 1001, 1235, 1330
HpaII CCGG 1 cut(s) 57
HphI GGTGA 4 cut(s) 46, 332, 826, 1025
Hpy166II GTNNAC 8 cut(s) 268, 288, 323, 751, 973, 1078, 1369, 1517
Hpy188I TCNGA 3 cut(s) 277, 736, 856
Hpy188III TCNNGA 6 cut(s) 488, 499, 797, 1093, 1245, 1273
Hpy8I GTNNAC 8 cut(s) 268, 288, 323, 751, 973, 1078, 1369, 1517
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 54, 272, 329, 356, 695
HpyCH4III ACNGT 3 cut(s) 871, 970, 1162
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
HpyF3I CTNAG 7 cut(s) 207, 329, 393, 807, 1008, 1336, 1439
Hsp92II CATG 6 cut(s) 146, 437, 699, 851, 1327, 1381
HspAI GCGC 2 cut(s) 782, 945
Kzo9I GATC 4 cut(s) 164, 793, 1108, 1311
LguI GCTCTTC 3 cut(s) 27, 28, 1636
LmnI GCTCC 4 cut(s) 63, 451, 568, 611
Lsp1109I GCAGC 3 cut(s) 589, 1042, 1266
LweI GCATC 4 cut(s) 182, 433, 889, 1137
MaeI CTAG 3 cut(s) 306, 417, 1649
MaeIII GTNAC 2 cut(s) 964, 985
MalI GATC 4 cut(s) 166, 795, 1110, 1313
MboI GATC 4 cut(s) 164, 793, 1108, 1311
MfeI CAATTG 1 cut(s) 1406
MflI RGATCY 1 cut(s) 164
MhlI GDGCHC 3 cut(s) 22, 290, 1631
MmeI TCCRAC 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 1125
MseI TTAA 9 cut(s) 314, 525, 630, 774, 830, 839, 926, 1026, 1167
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 1 cut(s) 1166
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 5 cut(s) 573, 1152, 1346, 1609, 1636
MunI CAATTG 1 cut(s) 1406
Mva1269I GAATGC 2 cut(s) 918, 1580
MvaI CCWGG 5 cut(s) 573, 1152, 1346, 1609, 1636
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
NdeI CATATG 1 cut(s) 949
NdeII GATC 4 cut(s) 164, 793, 1108, 1311
NlaIII CATG 6 cut(s) 146, 437, 699, 851, 1327, 1381
NlaIV GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 804, 1047
NmeAIII GCCGAG 1 cut(s) 362
NmuCI GTSAC 1 cut(s) 985
NsbI TGCGCA 1 cut(s) 783
PceI AGGCCT 1 cut(s) 353
PciSI GCTCTTC 3 cut(s) 27, 28, 1636
PctI GAATGC 2 cut(s) 918, 1580
PdmI GAANNNNTTC 1 cut(s) 1125
PfeI GAWTC 4 cut(s) 233, 1001, 1235, 1330
PflMI CCANNNNNTGG 1 cut(s) 455
PfoI TCCNGGA 1 cut(s) 1607
PkrI GCNGC 6 cut(s) 68, 461, 604, 944, 1057, 1256
PsiI TTATAA 1 cut(s) 378
Psp124BI GAGCTC 2 cut(s) 22, 1631
Psp6I CCWGG 5 cut(s) 571, 1150, 1344, 1607, 1634
PspGI CCWGG 5 cut(s) 571, 1150, 1344, 1607, 1634
PspN4I GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 804, 1047
PspPI GGNCC 1 cut(s) 1534
PstI CTGCAG 2 cut(s) 706, 1060
PsuI RGATCY 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 5 cut(s) 580, 587, 683, 1125, 1518
RsaNI GTAC 5 cut(s) 579, 586, 682, 1124, 1517
RseI CAYNNNNRTG 1 cut(s) 159
SacI GAGCTC 2 cut(s) 22, 1631
SapI GCTCTTC 3 cut(s) 27, 28, 1636
SaqAI TTAA 9 cut(s) 314, 525, 630, 774, 830, 839, 926, 1026, 1167
SatI GCNGC 6 cut(s) 67, 460, 603, 943, 1056, 1255
Sau3AI GATC 4 cut(s) 164, 793, 1108, 1311
Sau96I GGNCC 1 cut(s) 1534
ScaI AGTACT 1 cut(s) 1125
ScrFI CCNGG 5 cut(s) 573, 1152, 1346, 1609, 1636
SduI GDGCHC 3 cut(s) 22, 290, 1631
SfaNI GCATC 4 cut(s) 182, 433, 889, 1137
SfcI CTRYAG 4 cut(s) 237, 702, 1056, 1479
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 6 cut(s) 797, 917, 1050, 1166, 1217, 1229
SmoI CTYRAG 6 cut(s) 797, 917, 1050, 1166, 1217, 1229
SpeI ACTAGT 2 cut(s) 305, 1648
SseBI AGGCCT 1 cut(s) 353
SsiI CCGC 3 cut(s) 67, 460, 942
SspI AATATT 3 cut(s) 772, 1030, 1503
SspMI CTAG 3 cut(s) 306, 417, 1649
SstI GAGCTC 2 cut(s) 22, 1631
StuI AGGCCT 1 cut(s) 353
StyD4I CCNGG 5 cut(s) 571, 1150, 1344, 1607, 1634
StyI CCWWGG 3 cut(s) 615, 642, 744
TaaI ACNGT 3 cut(s) 871, 970, 1162
TaqI TCGA 1 cut(s) 1272
TatI WGTACW 3 cut(s) 585, 1123, 1516
TauI GCSGC 3 cut(s) 69, 462, 945
TfiI GAWTC 4 cut(s) 233, 1001, 1235, 1330
Tru1I TTAA 9 cut(s) 314, 525, 630, 774, 830, 839, 926, 1026, 1167
Tru9I TTAA 9 cut(s) 314, 525, 630, 774, 830, 839, 926, 1026, 1167
TscAI CASTG 3 cut(s) 235, 880, 1591
TseFI GTSAC 1 cut(s) 985
TseI GCWGC 3 cut(s) 602, 1055, 1254
Tsp45I GTSAC 1 cut(s) 985
TspDTI ATGAA 8 cut(s) 216, 237, 450, 507, 1098, 1118, 1182, 1580
TspGWI ACGGA 2 cut(s) 165, 1178
TspRI CASTG 3 cut(s) 235, 880, 1591
Van91I CCANNNNNTGG 1 cut(s) 455
Vha464I CTTAAG 1 cut(s) 1166
VneI GTGCAC 1 cut(s) 286
XapI RAATTY 5 cut(s) 494, 885, 1240, 1268, 1613
XmiI GTMKAC 2 cut(s) 267, 750
XmnI GAANNNNTTC 1 cut(s) 1125
XspI CTAG 3 cut(s) 306, 417, 1649
ZrmI AGTACT 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.