Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003815

Physical Location & Seq

Genomic Coordinates Reverse (-)

627113 .. 632392

Gene Structure 5280 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1003000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1839 bp

ATGCAGAGATCACGTAGAGCTCTTCTGAACAGAAGAGCTTTAGGGATCAGTGGAAGGAACCGCTTGTATAAGGTGTCTCTGTCTTTAGTTTTCGTCCTTTGGGGGCTTGTCTTCCTTTTCAGCTTATGGTTCAGTCGTGGACATGGATACAAAGATGGATCCACTGTATCTCCAGTTGGTATATCAACTTGGGATGAAGCTAAGCTGGATCGTGATGAACACTATGATATACAAAAAGAATCTGATTTGGGCTACTCTTCTGGAGGTGAGTGCACAAATGGGGTTGAAACCGGTGGTTTAAATGGTGAGTTCTTTGCTATGGAAGGAAGTAAACAGCATGCCTCTGCTGAAGGAAGTAGACAGCAAGATTTAGCTGAAGGAAGTCTACATCATGCCTCAACTGAAGGAAGTATCTTTCATGACTCAGCTGTGGATGAGCAACCTGAGGTGGTGACTGCTGGTTCAGGTGTAAAACTTGAAAATGATGCTCCAAAGAATGGTCGGTTGCCTCGTGCTGTGCCTCTTGGTCTTGATGAATTCAAGAGCAAAACATTTAGTTCCAAAAGTAAATCTGGAAATGGTCAGGCTGGAGGCATAAAACATAGAGTGGAGCCTGGTGGTGCAGAGTACAATTATGCGTCAGCTGCAAAGGGAGCCAAGGTCTTGGCTTTCAACAAGGAAGCTAAGGGTGCCTCTAATATCTTAGGCAAAGACAAAGACAAGTACCTTCGCAATCCATGTTCTGCAGAAGGAAAGTTTGTCGATATAGAGCTTTCTGAAGAAACCTTGGTAGATACAATTGAAATAGCTAATCTTGAGCACTATTCGTCTAATTTAAAAGATTTTGAGGTGCTCGGCAGTTTGACTTATCCAACAAATGAATGGGTCTTTCTTGGGAATGTTACTGCTGCAAATAATAAACTTGTACAAAGGTTTGTTCTTCAGCAACCCAAGTGGGTGAGATATATAAAGTTGAAACTTTTGAGCCATTATGGTTCAGAATTCTACTGCACACTTAGTATTATTGAACTTTATGGAGTCGATGCTGTTGAGCGAATGCTTGAGGATTTGATTTCTGTTGAAAGCAGTTCGTTTGTTTCTGAGGGAGCAACAGTTGACCAGAAACCTGTACCCTCACACCCGGACTCCCCTGAGGTTGATGAATTTTTTCATGATATTGTTAAAGAATCGGAACCCCAATATGCAGCTGGAGTGTCTAATGTGAATAATGATATGATGAATAGTGAAGTGCCTGATCCTGTCAAAGAAGTTCGTCATCAACAAGTTAATAGGATGCCTGGGGACACAGTTCTGAAAATTTTAATGCAGAAAGTTCGTTCATTAGATTTCAGTTTATCAGTTCTGGAGCGATACCTGGAGGAATCAACTTCAAAATATGGCAGTATTTTTGGAGAATTTGACAAGGATTTGGGAGAGAAAGGTACGGATTTACAGAAGATCAGAGAAGACATAAGGAATCTCGTTCAAAGCCAGGAAGTCATTGCTAAAGATGTTCACAATCTTATATCTTGGCAGTCCCTAGTTACCATGCAATTGAATAATCTAGTCAGGGACAATGCTATCCTCAGATCTGAGGTTGAAAAGGTGAGGGAGAAGCAGATATCTGTAGATAATAAGGGTATCTTAATATTTCTTATATGCATAATATTTTCTTTGTTAGCTCTTGTGAGGCTTTTCACAGAAATGGCGGTTAGTGTTTATATGGTATTGAGTGTTGATAGAGCAACAGAAAAGCCGAGGAAATTTTGTTGGATGAGTTCTTCCTGGCTTTTTTTACTTGTGAGCTGTATCCTTGTCCTGTTTATATTATCATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0006996 GO:0006997 GO:0008150 GO:0009987 GO:0012505 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sad1_UNC

Protein Analysis

613

Amino Acids

68.25

Weight (kDa)

5.56

Isoelectric Point (pI)

40.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sad1_UNC	PF07738	225 - 347	2.4e-33	Sad1 / UNC-like C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22882 AT1G71360 AT1G71360
fragaria_vesca	FvH4_2g00480 FvH4_2g00480 FvH4_2g00480 FvH4_2g00480 FvH4_2g00480 FvH4_2g00480 FvH4_2g00480 FvH4_2g00480
malus_domestica	MD05G1001800.v1.1 MD10G1003000.v1.1
prunus_persica	Prupe.8G004700_v2.0.a1 Prupe.8G004700_v2.0.a1 Prupe.8G004700_v2.0.a1 Prupe.8G004700_v2.0.a1
pyrus_communis	pycom05g00200 pycom10g00240
rosa_chinensis	RchiOBHm_Chr6g0250471
rosa_laevigata	RLG00000015538
rosa_multiflora	Rmu_sc0001209.1_g000035
rosa_roxburghii	Rroxscaffold_7G00217210
rosa_rugosa	Rorug05G0497300 Rorug05G0497400 Rorug05G0497400 Rorug05G0497400
rosa_samantha	Rh6AG006100 Rh6BG004600 Rh6CG004300 Rh6DG004200
rosa_wichuraiana	Rw0G015440 Rw6G000660 Rw6G000730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1837
AccB1I	GGYRCC	1 cut(s)	689
AccB7I	CCANNNNNTGG	1 cut(s)	497
AccI	GTMKAC	2 cut(s)	358, 385
AciI	CCGC	2 cut(s)	61, 1709
AclWI	GGATC	5 cut(s)	53, 153, 166, 216, 1250
AcsI	RAATTY	6 cut(s)	536, 1001, 1163, 1317, 1415, 1763
AcuI	CTGAAG	5 cut(s)	369, 396, 423, 798, 926
AfaI	GTAC	5 cut(s)	629, 725, 927, 1131, 1444
AfiI	CCNNNNNNNGG	1 cut(s)	497
AgeI	ACCGGT	1 cut(s)	290
AjnI	CCWGG	5 cut(s)	613, 1297, 1374, 1491, 1784
Alw21I	GWGCWC	4 cut(s)	22, 275, 822, 855
Alw26I	GTCTC	1 cut(s)	81
Alw44I	GTGCAC	1 cut(s)	271
AlwI	GGATC	5 cut(s)	53, 153, 166, 216, 1250
ApaLI	GTGCAC	1 cut(s)	271
ApeKI	GCWGC	3 cut(s)	644, 908, 1205
ApoI	RAATTY	6 cut(s)	536, 1001, 1163, 1317, 1415, 1763
AsiGI	ACCGGT	1 cut(s)	290
Asp700I	GAANNNNTTC	1 cut(s)	1167
AsuC2I	CCSGG	1 cut(s)	1142
AsuHPI	GGTGA	5 cut(s)	278, 317, 463, 970, 1618
AxyI	CCTNAGG	2 cut(s)	444, 1152
BaeGI	GKGCMC	1 cut(s)	275
BamHI	GGATCC	1 cut(s)	158
BanI	GGYRCC	1 cut(s)	689
BanII	GRGCYC	1 cut(s)	22
BarI	GAAGNNNNNNTAC	2 cut(s)	369, 401
BauI	CACGAG	1 cut(s)	510
BbsI	GAAGAC	2 cut(s)	103, 1473
Bbv12I	GWGCWC	4 cut(s)	22, 275, 822, 855
BbvI	GCAGC	3 cut(s)	631, 895, 1217
BccI	CCATC	1 cut(s)	149
BcgI	CGANNNNNNTGC	2 cut(s)	1316, 1350
BciT130I	CCWGG	5 cut(s)	615, 1299, 1376, 1493, 1786
BciVI	GTATCC	2 cut(s)	140, 1820
BcnI	CCSGG	1 cut(s)	1142
BcoDI	GTCTC	1 cut(s)	81
BfaI	CTAG	2 cut(s)	1541, 1565
BfmI	CTRYAG	2 cut(s)	744, 1626
BfuI	GTATCC	2 cut(s)	140, 1820
BglII	AGATCT	1 cut(s)	1589
BisI	GCNGC	3 cut(s)	645, 909, 1206
BlpI	GCTNAGC	1 cut(s)	201
BlsI	GCNGC	3 cut(s)	646, 910, 1207
Bme1390I	CCNGG	6 cut(s)	615, 1142, 1299, 1376, 1493, 1786
BmiI	GGNNCC	6 cut(s)	59, 160, 612, 655, 691, 1194
BmrFI	CCNGG	6 cut(s)	615, 1142, 1299, 1376, 1493, 1786
BmsI	GCATC	3 cut(s)	475, 1033, 1284
BpiI	GAAGAC	2 cut(s)	103, 1473
BpmI	CTGGAG	6 cut(s)	156, 282, 609, 1230, 1385, 1397
Bpu10I	CCTNAGC	1 cut(s)	684
Bpu1102I	GCTNAGC	1 cut(s)	201
BpuEI	CTTGAG	2 cut(s)	836, 1082
BpuMI	CCSGG	1 cut(s)	1142
BsaAI	YACGTR	1 cut(s)	14
BsaJI	CCNNGG	4 cut(s)	657, 786, 1298, 1757
BsaWI	WCCGGW	1 cut(s)	290
BsaXI	ACNNNNNCTCC	8 cut(s)	154, 184, 645, 675, 1098, 1128, 1130, 1160
Bsc4I	CCNNNNNNNGG	1 cut(s)	497
Bse118I	RCCGGY	1 cut(s)	290
Bse1I	ACTGG	1 cut(s)	173
Bse21I	CCTNAGG	2 cut(s)	444, 1152
Bse3DI	GCAATG	1 cut(s)	1500
BseBI	CCWGG	5 cut(s)	615, 1299, 1376, 1493, 1786
BseDI	CCNNGG	4 cut(s)	657, 786, 1298, 1757
BseGI	GGATG	4 cut(s)	199, 439, 1299, 1779
BseLI	CCNNNNNNNGG	1 cut(s)	497
BseMI	GCAATG	1 cut(s)	1500
BseMII	CTCAG	6 cut(s)	435, 438, 1092, 1143, 1584, 1600
BseNI	ACTGG	1 cut(s)	173
BseSI	GKGCMC	1 cut(s)	275
BseXI	GCAGC	3 cut(s)	631, 895, 1217
BsgI	GTGCAG	2 cut(s)	642, 994
BshNI	GGYRCC	1 cut(s)	689
BshTI	ACCGGT	1 cut(s)	290
BsiHKAI	GWGCWC	4 cut(s)	22, 275, 822, 855
BsiSI	CCGG	2 cut(s)	291, 1142
BslFI	GGGAC	3 cut(s)	1316, 1522, 1586
BslI	CCNNNNNNNGG	1 cut(s)	497
BsmAI	GTCTC	1 cut(s)	81
BsmFI	GGGAC	3 cut(s)	1316, 1522, 1586
BsmI	GAATGC	1 cut(s)	1062
Bsp1286I	GDGCHC	4 cut(s)	22, 275, 822, 855
Bsp1407I	TGTACA	1 cut(s)	925
Bsp143I	GATC	7 cut(s)	8, 45, 158, 208, 1255, 1458, 1589
Bsp1720I	GCTNAGC	1 cut(s)	201
BspACI	CCGC	2 cut(s)	61, 1709
BspCNI	CTCAG	6 cut(s)	436, 437, 1093, 1144, 1585, 1599
BspHI	TCATGA	2 cut(s)	418, 1171
BspLI	GGNNCC	6 cut(s)	59, 160, 612, 655, 691, 1194
BspMAI	CTGCAG	1 cut(s)	748
BspPI	GGATC	5 cut(s)	53, 153, 166, 216, 1250
BspQI	GCTCTTC	2 cut(s)	27, 28
BspT107I	GGYRCC	1 cut(s)	689
BsrDI	GCAATG	1 cut(s)	1500
BsrFI	RCCGGY	1 cut(s)	290
BsrGI	TGTACA	1 cut(s)	925
BsrI	ACTGG	1 cut(s)	173
BssAI	RCCGGY	1 cut(s)	290
BssECI	CCNNGG	4 cut(s)	657, 786, 1298, 1757
BssMI	GATC	7 cut(s)	8, 45, 158, 208, 1255, 1458, 1589
BssSI	CACGAG	1 cut(s)	510
BssT1I	CCWWGG	2 cut(s)	657, 786
Bst2BI	CACGAG	1 cut(s)	510
Bst2UI	CCWGG	5 cut(s)	615, 1299, 1376, 1493, 1786
Bst4CI	ACNGT	3 cut(s)	166, 1114, 1309
Bst6I	CTCTTC	3 cut(s)	27, 28, 262
BstAUI	TGTACA	1 cut(s)	925
BstBAI	YACGTR	1 cut(s)	14
BstC8I	GCNNGC	1 cut(s)	339
BstF5I	GGATG	4 cut(s)	199, 439, 1299, 1779
BstKTI	GATC	7 cut(s)	11, 48, 161, 211, 1258, 1461, 1592
BstMAI	GTCTC	1 cut(s)	81
BstMBI	GATC	7 cut(s)	8, 45, 158, 208, 1255, 1458, 1589
BstMWI	GCNNNNNNNGC	3 cut(s)	644, 653, 689
BstNI	CCWGG	5 cut(s)	615, 1299, 1376, 1493, 1786
BstNSI	RCATGY	1 cut(s)	341
BstSCI	CCNGG	6 cut(s)	613, 1140, 1297, 1374, 1491, 1784
BstSFI	CTRYAG	2 cut(s)	744, 1626
BstSLI	GKGCMC	1 cut(s)	275
BstV1I	GCAGC	3 cut(s)	631, 895, 1217
BstV2I	GAAGAC	2 cut(s)	103, 1473
BstX2I	RGATCY	2 cut(s)	158, 1589
BstXI	CCANNNNNNTGG	1 cut(s)	664
BstYI	RGATCY	2 cut(s)	158, 1589
Bsu36I	CCTNAGG	2 cut(s)	444, 1152
BsuI	GTATCC	2 cut(s)	140, 1820
BtsCI	GGATG	4 cut(s)	199, 439, 1299, 1779
BtsIMutI	CAGTG	2 cut(s)	55, 162
Cac8I	GCNNGC	1 cut(s)	339
CciI	TCATGA	2 cut(s)	418, 1171
Cfr10I	RCCGGY	1 cut(s)	290
CseI	GACGC	1 cut(s)	627
Csp6I	GTAC	5 cut(s)	628, 724, 926, 1130, 1443
CspAI	ACCGGT	1 cut(s)	290
CspCI	CAANNNNNGTGG	2 cut(s)	935, 970
CviAII	CATG	7 cut(s)	143, 338, 392, 419, 738, 1172, 1549
CviQI	GTAC	5 cut(s)	628, 724, 926, 1130, 1443
DpnI	GATC	7 cut(s)	10, 47, 160, 210, 1257, 1460, 1591
DpnII	GATC	7 cut(s)	8, 45, 158, 208, 1255, 1458, 1589
DraI	TTTAAA	2 cut(s)	300, 837
Eam1104I	CTCTTC	3 cut(s)	27, 28, 262
EarI	CTCTTC	3 cut(s)	27, 28, 262
Ecl136II	GAGCTC	1 cut(s)	20
Eco130I	CCWWGG	2 cut(s)	657, 786
Eco24I	GRGCYC	1 cut(s)	22
Eco32I	GATATC	1 cut(s)	1623
Eco53kI	GAGCTC	1 cut(s)	20
Eco57I	CTGAAG	5 cut(s)	369, 396, 423, 798, 926
Eco81I	CCTNAGG	2 cut(s)	444, 1152
EcoICRI	GAGCTC	1 cut(s)	20
EcoRI	GAATTC	2 cut(s)	536, 1001
EcoRII	CCWGG	5 cut(s)	613, 1297, 1374, 1491, 1784
EcoRV	GATATC	1 cut(s)	1623
EcoT14I	CCWWGG	2 cut(s)	657, 786
EcoT22I	ATGCAT	1 cut(s)	1664
EcoT38I	GRGCYC	1 cut(s)	22
ErhI	CCWWGG	2 cut(s)	657, 786
FaeI	CATG	7 cut(s)	146, 341, 395, 422, 741, 1175, 1552
FalI	AAGNNNNNCTT	2 cut(s)	1628, 1660
FaqI	GGGAC	3 cut(s)	1316, 1522, 1586
FatI	CATG	7 cut(s)	142, 337, 391, 418, 737, 1171, 1548
FblI	GTMKAC	2 cut(s)	358, 385
Fnu4HI	GCNGC	3 cut(s)	645, 909, 1206
FokI	GGATG	4 cut(s)	206, 446, 1306, 1786
FriOI	GRGCYC	1 cut(s)	22
Fsp4HI	GCNGC	3 cut(s)	645, 909, 1206
FspBI	CTAG	2 cut(s)	1541, 1565
GluI	GCNGC	3 cut(s)	645, 909, 1206
GsuI	CTGGAG	6 cut(s)	156, 282, 609, 1230, 1385, 1397
HapII	CCGG	2 cut(s)	291, 1142
HgaI	GACGC	1 cut(s)	627
Hin1II	CATG	7 cut(s)	146, 341, 395, 422, 741, 1175, 1552
HincII	GTYRAC	1 cut(s)	1117
HindII	GTYRAC	1 cut(s)	1117
HinfI	GANTC	7 cut(s)	239, 422, 1038, 1145, 1187, 1382, 1477
HpaII	CCGG	2 cut(s)	291, 1142
HphI	GGTGA	5 cut(s)	278, 317, 463, 970, 1618
Hpy166II	GTNNAC	7 cut(s)	140, 273, 332, 359, 386, 1117, 1516
Hpy188III	TCNNGA	9 cut(s)	212, 261, 419, 530, 541, 573, 815, 1172, 1364
Hpy8I	GTNNAC	7 cut(s)	140, 273, 332, 359, 386, 1117, 1516
HpyAV	CCTTC	7 cut(s)	48, 317, 344, 371, 398, 737, 743
HpyCH4III	ACNGT	3 cut(s)	166, 1114, 1309
HpyCH4IV	ACGT	1 cut(s)	13
HpyF10VI	GCNNNNNNNGC	3 cut(s)	644, 653, 689
HpySE526I	ACGT	1 cut(s)	13
Hsp92II	CATG	7 cut(s)	146, 341, 395, 422, 741, 1175, 1552
Kzo9I	GATC	7 cut(s)	8, 45, 158, 208, 1255, 1458, 1589
LguI	GCTCTTC	2 cut(s)	27, 28
LmnI	GCTCC	5 cut(s)	493, 610, 653, 1106, 1366
Lsp1109I	GCAGC	3 cut(s)	631, 895, 1217
LweI	GCATC	3 cut(s)	475, 1033, 1284
MaeI	CTAG	2 cut(s)	1541, 1565
MaeII	ACGT	1 cut(s)	13
MaeIII	GTNAC	3 cut(s)	451, 901, 1543
MalI	GATC	7 cut(s)	10, 47, 160, 210, 1257, 1460, 1591
MboI	GATC	7 cut(s)	8, 45, 158, 208, 1255, 1458, 1589
MboII	GAAGA	9 cut(s)	14, 45, 103, 249, 791, 932, 1468, 1478, 1773
MfeI	CAATTG	2 cut(s)	798, 1553
MflI	RGATCY	2 cut(s)	158, 1589
MhlI	GDGCHC	4 cut(s)	22, 275, 822, 855
MlyI	GAGTC	3 cut(s)	416, 1047, 1139
MmeI	TCCRAC	2 cut(s)	896, 1751
Mph1103I	ATGCAT	1 cut(s)	1664
MroXI	GAANNNNTTC	1 cut(s)	1167
MseI	TTAA	6 cut(s)	299, 836, 1182, 1287, 1322, 1646
MslI	CAYNNNNRTG	1 cut(s)	1703
MspA1I	CMGCKG	3 cut(s)	428, 644, 1208
MspI	CCGG	2 cut(s)	291, 1142
MspR9I	CCNGG	6 cut(s)	615, 1142, 1299, 1376, 1493, 1786
MunI	CAATTG	2 cut(s)	798, 1553
Mva1269I	GAATGC	1 cut(s)	1062
MvaI	CCWGG	5 cut(s)	615, 1299, 1376, 1493, 1786
MwoI	GCNNNNNNNGC	3 cut(s)	644, 653, 689
NciI	CCSGG	1 cut(s)	1142
NdeII	GATC	7 cut(s)	8, 45, 158, 208, 1255, 1458, 1589
NlaIII	CATG	7 cut(s)	146, 341, 395, 422, 741, 1175, 1552
NlaIV	GGNNCC	6 cut(s)	59, 160, 612, 655, 691, 1194
NmeAIII	GCCGAG	2 cut(s)	834, 1782
NmuCI	GTSAC	1 cut(s)	451
NsiI	ATGCAT	1 cut(s)	1664
NspI	RCATGY	1 cut(s)	341
PaeI	GCATGC	1 cut(s)	341
PagI	TCATGA	2 cut(s)	418, 1171
PciSI	GCTCTTC	2 cut(s)	27, 28
PcsI	WCGNNNNNNNCGW	1 cut(s)	508
PctI	GAATGC	1 cut(s)	1062
PdmI	GAANNNNTTC	1 cut(s)	1167
PfeI	GAWTC	4 cut(s)	239, 1187, 1382, 1477
PflMI	CCANNNNNTGG	1 cut(s)	497
PinAI	ACCGGT	1 cut(s)	290
PkrI	GCNGC	3 cut(s)	646, 910, 1207
PleI	GAGTC	3 cut(s)	416, 1046, 1139
PpsI	GAGTC	3 cut(s)	416, 1046, 1139
Ppu21I	YACGTR	1 cut(s)	14
PsiI	TTATAA	1 cut(s)	1837
Psp124BI	GAGCTC	1 cut(s)	22
Psp6I	CCWGG	5 cut(s)	613, 1297, 1374, 1491, 1784
PspGI	CCWGG	5 cut(s)	613, 1297, 1374, 1491, 1784
PspN4I	GGNNCC	6 cut(s)	59, 160, 612, 655, 691, 1194
PstI	CTGCAG	1 cut(s)	748
PsuI	RGATCY	2 cut(s)	158, 1589
PvuII	CAGCTG	3 cut(s)	428, 644, 1208
RsaI	GTAC	5 cut(s)	629, 725, 927, 1131, 1444
RsaNI	GTAC	5 cut(s)	628, 724, 926, 1130, 1443
RseI	CAYNNNNRTG	1 cut(s)	1703
SacI	GAGCTC	1 cut(s)	22
SapI	GCTCTTC	2 cut(s)	27, 28
SaqAI	TTAA	6 cut(s)	299, 836, 1182, 1287, 1322, 1646
SatI	GCNGC	3 cut(s)	645, 909, 1206
Sau3AI	GATC	7 cut(s)	8, 45, 158, 208, 1255, 1458, 1589
SchI	GAGTC	3 cut(s)	416, 1047, 1139
ScrFI	CCNGG	6 cut(s)	615, 1142, 1299, 1376, 1493, 1786
SduI	GDGCHC	4 cut(s)	22, 275, 822, 855
SfaNI	GCATC	3 cut(s)	475, 1033, 1284
SfcI	CTRYAG	2 cut(s)	744, 1626
SmiMI	CAYNNNNRTG	1 cut(s)	1703
SmlI	CTYRAG	2 cut(s)	815, 1061
SmoI	CTYRAG	2 cut(s)	815, 1061
SphI	GCATGC	1 cut(s)	341
SsiI	CCGC	2 cut(s)	61, 1709
SspI	AATATT	2 cut(s)	1650, 1668
SspMI	CTAG	2 cut(s)	1541, 1565
SstI	GAGCTC	1 cut(s)	22
StyD4I	CCNGG	6 cut(s)	613, 1140, 1297, 1374, 1491, 1784
StyI	CCWWGG	2 cut(s)	657, 786
TaaI	ACNGT	3 cut(s)	166, 1114, 1309
TaiI	ACGT	1 cut(s)	16
TaqI	TCGA	2 cut(s)	762, 1041
TatI	WGTACW	2 cut(s)	627, 925
TfiI	GAWTC	4 cut(s)	239, 1187, 1382, 1477
Tru1I	TTAA	6 cut(s)	299, 836, 1182, 1287, 1322, 1646
Tru9I	TTAA	6 cut(s)	299, 836, 1182, 1287, 1322, 1646
TscAI	CASTG	2 cut(s)	55, 169
TseFI	GTSAC	1 cut(s)	451
TseI	GCWGC	3 cut(s)	644, 908, 1205
Tsp45I	GTSAC	1 cut(s)	451
TspDTI	ATGAA	9 cut(s)	210, 231, 407, 549, 894, 1160, 1176, 1253, 1329
TspGWI	ACGGA	1 cut(s)	1460
TspRI	CASTG	2 cut(s)	55, 169
Van91I	CCANNNNNTGG	1 cut(s)	497
VneI	GTGCAC	1 cut(s)	271
XapI	RAATTY	6 cut(s)	536, 1001, 1163, 1317, 1415, 1763
XceI	RCATGY	1 cut(s)	341
XcmI	CCANNNNNNNNNTGG	2 cut(s)	879, 1205
XmiI	GTMKAC	2 cut(s)	358, 385
XmnI	GAANNNNTTC	1 cut(s)	1167
XspI	CTAG	2 cut(s)	1541, 1565
Zsp2I	ATGCAT	1 cut(s)	1664

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.