Rh6AG006100

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003815
Physical Location & Seq
Reverse (-)
785770 .. 790678
4909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG006100.1

Sequence Viewer

Length: 1548 bp
ATGCAGAGGTCGCGTAGAGCTCTTCTGCATAGAAGAGCTTTGGAAAAGGTCATCACCGGAAGGAGCCGCTTGTATAAGGTGTCTCTATCTTTAGTTTTTGCTCTGTGGGGGTTTGTCTTCCTTATCAGCTTATGGTTTAGTCGTGGCGACGGACACAGAGATGGATCCACAGCATCTCCAGTTGGATTATCAACTTGGAATGAAACTAAGCTGGATCGTGATGAACACACTGATTCTGTAGAACTACAAAAGCAAGAAGATTTAGTCTACTCTTCTGAAGGTGTGTGCACAAATGGTGTTGAAACTAGTAGTTTAAATGCTGAACTACTTAGTGAAGGAAATAAACATCAGGCCTCAGCAGAAGGAAGTGCAATTTATAACTCGGCCGTAGCTGAGCAACCTGAATTGGAGAAATCTAGTTCAGGCGTGAAACATGAAATTGATGCTCCAAAAAATGGCCGCCTGCCTCGTGCTGTGCCTCTTGGTCTGGATGAATTCAAGAGCAAAACATTTAGTTCAAAAATTAAATCTGTAATTGGTCAGGCTGGAGGCATAAAACATAGAGTGGAGCCTGGTGGTGCAGAGTACAATTACGCTTCAGCTGCAAAGGGAGCCAAGGTCTTGGCTTTTAACAAGGAAGCCAAGGGTGCCTCTAATATCATAAGCAGAGACAAGGACAAGTACCTTCGCAATCCATGTTCTGCAGAAGAAAAGTTTGTTGATATAGAACTTTCAGAAGAAACCTTGGTAGACACAATCAAAATAGGTAATCTTGAACACTATTCATCTAATTTAAGGGATTTTGAGCTGCTTGGCAGTTTGGTTTATCCAACAGATGAATGGGTTAAGCTTGGGAATTTCACTGCAGCAAATATTAAGCTTGCGCAAAGATTTGATCTTGAGGAACCTAAGTGGGTGAGATATATAAGGTTAAAGCTTTTAAACCATTATGGTTCAGAGTTCTATTGCACAGTCAGTGTTATTGAAATTTATGGAATTGATGCTGTTGAGCGAATGCTTGAGGATTTAATTTCTGTTGAAAGCGGCGCATATGTTTCTAATGGAGTAACTGTTGACCAGAAACCTGTGACCTCCCACCCAGATTCCCCTGAGGGTGATGATTTTTTTAATATTGATAAAGAAATGGAACCTCAAGCTGCAGTTGAAAACTCTAATGTGAACAATGAAATAATCAAGAGTGATGTGCCTGATCCTATCAAAGAAGCACTTCATCAACAAGGTAGCAGGATGCCTGGGGATACTGTTCTTAAGATACTAATGCAGAAAGTCCGTTCATTGGATTTTAGTTTATCACTACTTGAGCGATACCTTGAGGAATCAAATTCAAGATACGGGAGCATTTTCAAAGAATTCGAGACAGATATGGAAGAGAAAGAGTTAGATTTACAGAAGATAAAAGAAAACATGAGGAATCTCTTAGAAAGCCAGGAAGTAATTGCTAAAGATGTAAACAATCTCATGTCTTGGCAGTCCCTTGTCTCTGTGCAATTGGATAATTTAGTCAGGGATAATGCTATCCTCAGGTGA

Protein Analysis

515

Amino Acids

57.78

Weight (kDa)

5.21

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 211 - 332 7.7e-32 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
Acc16I TGCGCA 1 cut(s) 885
AccB1I GGYRCC 1 cut(s) 647
AccB7I CCANNNNNTGG 1 cut(s) 455
AccI GTMKAC 2 cut(s) 267, 750
AccII CGCG 1 cut(s) 13
AciI CCGC 3 cut(s) 67, 460, 1044
AclWI GGATC 4 cut(s) 159, 172, 222, 1205
AcoI YGGCCR 2 cut(s) 384, 457
AcsI RAATTY 5 cut(s) 494, 856, 987, 1342, 1370
AcuI CTGAAG 2 cut(s) 297, 582
AfaI GTAC 2 cut(s) 587, 683
AfiI CCNNNNNNNGG 2 cut(s) 455, 1297
AflII CTTAAG 1 cut(s) 1268
AgsI TTSAA 9 cut(s) 302, 499, 519, 776, 986, 1040, 1166, 1347, 1366
AhlI ACTAGT 1 cut(s) 305
AjnI CCWGG 3 cut(s) 571, 1252, 1446
Alw21I GWGCWC 2 cut(s) 22, 290
Alw26I GTCTC 4 cut(s) 87, 663, 1370, 1504
Alw44I GTGCAC 1 cut(s) 286
AlwI GGATC 4 cut(s) 159, 172, 222, 1205
AoxI GGCC 3 cut(s) 351, 384, 457
ApaLI GTGCAC 1 cut(s) 286
ApeKI GCWGC 4 cut(s) 602, 808, 866, 1157
ApoI RAATTY 5 cut(s) 494, 856, 987, 1342, 1370
Asp700I GAANNNNTTC 1 cut(s) 1227
AspLEI GCGC 2 cut(s) 886, 1049
AsuHPI GGTGA 3 cut(s) 46, 928, 1127
AxyI CCTNAGG 2 cut(s) 1110, 1541
BaeGI GKGCMC 1 cut(s) 290
BamHI GGATCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 1 cut(s) 22
BauI CACGAG 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 2 cut(s) 22, 290
BbvCI CCTCAGC 1 cut(s) 355
BbvI GCAGC 4 cut(s) 589, 795, 878, 1144
BccI CCATC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 371
BciT130I CCWGG 3 cut(s) 573, 1254, 1448
BciVI GTATCC 1 cut(s) 1252
BcoDI GTCTC 4 cut(s) 87, 663, 1370, 1504
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 2 cut(s) 306, 417
BfmI CTRYAG 4 cut(s) 237, 702, 864, 1158
BfrI CTTAAG 1 cut(s) 1268
BfuI GTATCC 1 cut(s) 1252
BisI GCNGC 7 cut(s) 67, 460, 603, 809, 867, 1045, 1158
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 7 cut(s) 68, 461, 604, 810, 868, 1046, 1159
Bme1390I CCNGG 3 cut(s) 573, 1254, 1448
BmiI GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 906, 1149
BmrFI CCNGG 3 cut(s) 573, 1254, 1448
BmsI GCATC 4 cut(s) 182, 433, 991, 1239
BpiI GAAGAC 1 cut(s) 109
BpmI CTGGAG 2 cut(s) 162, 567
Bpu10I CCTNAGC 1 cut(s) 355
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 5 cut(s) 920, 1040, 1137, 1340, 1352
BsaJI CCNNGG 4 cut(s) 615, 642, 744, 1253
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 6 cut(s) 160, 190, 603, 633, 1056, 1086
Bsc4I CCNNNNNNNGG 2 cut(s) 455, 1297
Bse1I ACTGG 1 cut(s) 179
Bse21I CCTNAGG 2 cut(s) 1110, 1541
BseBI CCWGG 3 cut(s) 573, 1254, 1448
BseDI CCNNGG 4 cut(s) 615, 642, 744, 1253
BseGI GGATG 2 cut(s) 496, 1254
BseLI CCNNNNNNNGG 2 cut(s) 455, 1297
BseMII CTCAG 3 cut(s) 369, 384, 1101
BseNI ACTGG 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 290
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 4 cut(s) 589, 795, 878, 1144
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 13
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 3 cut(s) 353, 386, 459
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 2 cut(s) 22, 290
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 1 cut(s) 1477
BslI CCNNNNNNNGG 2 cut(s) 455, 1297
BsmAI GTCTC 4 cut(s) 87, 663, 1370, 1504
BsmFI GGGAC 1 cut(s) 1477
BsmI GAATGC 1 cut(s) 1020
BsnI GGCC 3 cut(s) 353, 386, 459
Bsp1286I GDGCHC 2 cut(s) 22, 290
Bsp143I GATC 4 cut(s) 164, 214, 895, 1210
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 3 cut(s) 67, 460, 1044
BspANI GGCC 3 cut(s) 353, 386, 459
BspCNI CTCAG 3 cut(s) 368, 385, 1102
BspFNI CGCG 1 cut(s) 13
BspLI GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 906, 1149
BspMAI CTGCAG 3 cut(s) 706, 868, 1162
BspPI GGATC 4 cut(s) 159, 172, 222, 1205
BspQI GCTCTTC 2 cut(s) 27, 28
BspT107I GGYRCC 1 cut(s) 647
BspTI CTTAAG 1 cut(s) 1268
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 4 cut(s) 615, 642, 744, 1253
BssMI GATC 4 cut(s) 164, 214, 895, 1210
BssSI CACGAG 1 cut(s) 468
BssT1I CCWWGG 3 cut(s) 615, 642, 744
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 3 cut(s) 573, 1254, 1448
Bst4CI ACNGT 3 cut(s) 973, 1072, 1264
Bst6I CTCTTC 4 cut(s) 27, 28, 277, 1383
BstAFI CTTAAG 1 cut(s) 1268
BstC8I GCNNGC 2 cut(s) 464, 882
BstDEI CTNAG 8 cut(s) 207, 329, 355, 393, 909, 1110, 1438, 1541
BstF5I GGATG 2 cut(s) 496, 1254
BstFNI CGCG 1 cut(s) 13
BstHHI GCGC 2 cut(s) 886, 1049
BstKTI GATC 4 cut(s) 167, 217, 898, 1213
BstMAI GTCTC 4 cut(s) 87, 663, 1370, 1504
BstMBI GATC 4 cut(s) 164, 214, 895, 1210
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
BstNI CCWGG 3 cut(s) 573, 1254, 1448
BstSCI CCNGG 3 cut(s) 571, 1252, 1446
BstSFI CTRYAG 4 cut(s) 237, 702, 864, 1158
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 4 cut(s) 589, 795, 878, 1144
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 164
BstXI CCANNNNNNTGG 1 cut(s) 622
BstYI RGATCY 1 cut(s) 164
BstZI CGGCCG 1 cut(s) 384
Bsu36I CCTNAGG 2 cut(s) 1110, 1541
BsuI GTATCC 1 cut(s) 1252
BsuRI GGCC 3 cut(s) 353, 386, 459
BtsCI GGATG 2 cut(s) 496, 1254
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 3 cut(s) 228, 861, 982
Cac8I GCNNGC 2 cut(s) 464, 882
CfoI GCGC 2 cut(s) 886, 1049
Csp6I GTAC 2 cut(s) 586, 682
CviAII CATG 4 cut(s) 434, 696, 1426, 1480
CviQI GTAC 2 cut(s) 586, 682
DdeI CTNAG 8 cut(s) 207, 329, 355, 393, 909, 1110, 1438, 1541
DpnI GATC 4 cut(s) 166, 216, 897, 1212
DpnII GATC 4 cut(s) 164, 214, 895, 1210
DraI TTTAAA 2 cut(s) 315, 942
EaeI YGGCCR 2 cut(s) 384, 457
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 4 cut(s) 27, 28, 277, 1383
EarI CTCTTC 4 cut(s) 27, 28, 277, 1383
Ecl136II GAGCTC 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 384
Eco130I CCWWGG 3 cut(s) 615, 642, 744
Eco147I AGGCCT 1 cut(s) 353
Eco24I GRGCYC 1 cut(s) 22
Eco52I CGGCCG 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 20
Eco57I CTGAAG 2 cut(s) 297, 582
Eco81I CCTNAGG 2 cut(s) 1110, 1541
EcoICRI GAGCTC 1 cut(s) 20
EcoRI GAATTC 2 cut(s) 494, 1370
EcoRII CCWGG 3 cut(s) 571, 1252, 1446
EcoT14I CCWWGG 3 cut(s) 615, 642, 744
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 3 cut(s) 615, 642, 744
FaeI CATG 4 cut(s) 437, 699, 1429, 1483
FalI AAGNNNNNCTT 2 cut(s) 1212, 1244
FaqI GGGAC 1 cut(s) 1477
FatI CATG 4 cut(s) 433, 695, 1425, 1479
FauNDI CATATG 1 cut(s) 1051
FblI GTMKAC 2 cut(s) 267, 750
Fnu4HI GCNGC 7 cut(s) 67, 460, 603, 809, 867, 1045, 1158
FokI GGATG 2 cut(s) 503, 1261
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 7 cut(s) 67, 460, 603, 809, 867, 1045, 1158
FspBI CTAG 2 cut(s) 306, 417
FspI TGCGCA 1 cut(s) 885
GlaI GCGC 2 cut(s) 885, 1048
GluI GCNGC 7 cut(s) 67, 460, 603, 809, 867, 1045, 1158
GsuI CTGGAG 2 cut(s) 162, 567
HaeIII GGCC 3 cut(s) 353, 386, 459
HapII CCGG 1 cut(s) 57
HhaI GCGC 2 cut(s) 886, 1049
Hin1II CATG 4 cut(s) 437, 699, 1429, 1483
Hin6I GCGC 2 cut(s) 884, 1047
HinP1I GCGC 2 cut(s) 884, 1047
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HindIII AAGCTT 3 cut(s) 848, 878, 935
HinfI GANTC 4 cut(s) 233, 1103, 1337, 1432
HpaII CCGG 1 cut(s) 57
HphI GGTGA 3 cut(s) 46, 928, 1127
Hpy166II GTNNAC 6 cut(s) 268, 288, 751, 1075, 1180, 1471
Hpy188I TCNGA 3 cut(s) 277, 736, 958
Hpy188III TCNNGA 8 cut(s) 218, 488, 499, 773, 899, 1195, 1347, 1375
Hpy8I GTNNAC 6 cut(s) 268, 288, 751, 1075, 1180, 1471
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 54, 272, 329, 356, 695
HpyCH4III ACNGT 3 cut(s) 973, 1072, 1264
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
HpyF3I CTNAG 8 cut(s) 207, 329, 355, 393, 909, 1110, 1438, 1541
Hsp92II CATG 4 cut(s) 437, 699, 1429, 1483
HspAI GCGC 2 cut(s) 884, 1047
Kzo9I GATC 4 cut(s) 164, 214, 895, 1210
LguI GCTCTTC 2 cut(s) 27, 28
LmnI GCTCC 5 cut(s) 63, 451, 568, 611, 1356
Lsp1109I GCAGC 4 cut(s) 589, 795, 878, 1144
LweI GCATC 4 cut(s) 182, 433, 991, 1239
MaeI CTAG 2 cut(s) 306, 417
MaeIII GTNAC 2 cut(s) 1066, 1087
MalI GATC 4 cut(s) 166, 216, 897, 1212
MboI GATC 4 cut(s) 164, 214, 895, 1210
MboII GAAGA 9 cut(s) 14, 45, 109, 264, 269, 719, 749, 1400, 1423
MfeI CAATTG 1 cut(s) 1508
MflI RGATCY 1 cut(s) 164
MhlI GDGCHC 2 cut(s) 22, 290
MmeI TCCRAC 2 cut(s) 163, 854
MroXI GAANNNNTTC 1 cut(s) 1227
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 1 cut(s) 1268
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 3 cut(s) 573, 1254, 1448
MunI CAATTG 1 cut(s) 1508
Mva1269I GAATGC 1 cut(s) 1020
MvaI CCWGG 3 cut(s) 573, 1254, 1448
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
NdeI CATATG 1 cut(s) 1051
NdeII GATC 4 cut(s) 164, 214, 895, 1210
NlaIII CATG 4 cut(s) 437, 699, 1429, 1483
NlaIV GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 906, 1149
NmeAIII GCCGAG 1 cut(s) 362
NmuCI GTSAC 1 cut(s) 1087
NsbI TGCGCA 1 cut(s) 885
PceI AGGCCT 1 cut(s) 353
PciSI GCTCTTC 2 cut(s) 27, 28
PctI GAATGC 1 cut(s) 1020
PdmI GAANNNNTTC 1 cut(s) 1227
PfeI GAWTC 4 cut(s) 233, 1103, 1337, 1432
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 7 cut(s) 68, 461, 604, 810, 868, 1046, 1159
PsiI TTATAA 1 cut(s) 378
Psp124BI GAGCTC 1 cut(s) 22
Psp6I CCWGG 3 cut(s) 571, 1252, 1446
PspGI CCWGG 3 cut(s) 571, 1252, 1446
PspN4I GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 906, 1149
PstI CTGCAG 3 cut(s) 706, 868, 1162
PsuI RGATCY 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 2 cut(s) 587, 683
RsaNI GTAC 2 cut(s) 586, 682
RseI CAYNNNNRTG 1 cut(s) 159
SacI GAGCTC 1 cut(s) 22
SapI GCTCTTC 2 cut(s) 27, 28
SatI GCNGC 7 cut(s) 67, 460, 603, 809, 867, 1045, 1158
Sau3AI GATC 4 cut(s) 164, 214, 895, 1210
ScrFI CCNGG 3 cut(s) 573, 1254, 1448
SduI GDGCHC 2 cut(s) 22, 290
SfaNI GCATC 4 cut(s) 182, 433, 991, 1239
SfcI CTRYAG 4 cut(s) 237, 702, 864, 1158
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 6 cut(s) 899, 1019, 1152, 1268, 1319, 1331
SmoI CTYRAG 6 cut(s) 899, 1019, 1152, 1268, 1319, 1331
SpeI ACTAGT 1 cut(s) 305
SseBI AGGCCT 1 cut(s) 353
SsiI CCGC 3 cut(s) 67, 460, 1044
SspI AATATT 2 cut(s) 874, 1132
SspMI CTAG 2 cut(s) 306, 417
SstI GAGCTC 1 cut(s) 22
StuI AGGCCT 1 cut(s) 353
StyD4I CCNGG 3 cut(s) 571, 1252, 1446
StyI CCWWGG 3 cut(s) 615, 642, 744
TaaI ACNGT 3 cut(s) 973, 1072, 1264
TaqI TCGA 1 cut(s) 1374
TatI WGTACW 1 cut(s) 585
TauI GCSGC 3 cut(s) 69, 462, 1047
TfiI GAWTC 4 cut(s) 233, 1103, 1337, 1432
TscAI CASTG 3 cut(s) 235, 868, 982
TseFI GTSAC 1 cut(s) 1087
TseI GCWGC 4 cut(s) 602, 808, 866, 1157
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 9 cut(s) 216, 237, 450, 507, 774, 852, 1200, 1220, 1284
TspGWI ACGGA 2 cut(s) 165, 1280
TspRI CASTG 3 cut(s) 235, 868, 982
Van91I CCANNNNNTGG 1 cut(s) 455
Vha464I CTTAAG 1 cut(s) 1268
VneI GTGCAC 1 cut(s) 286
XapI RAATTY 5 cut(s) 494, 856, 987, 1342, 1370
XcmI CCANNNNNNNNNTGG 1 cut(s) 837
XmiI GTMKAC 2 cut(s) 267, 750
XmnI GAANNNNTTC 1 cut(s) 1227
XspI CTAG 2 cut(s) 306, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.