RLG00000015538

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003815
Physical Location & Seq
Forward (+)
67630911 .. 67636019
5109 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015538

Sequence Viewer

Length: 1488 bp
ATGCAGAGGTCGCGTAGAGCTCTTCTGCATAGAAGAGCTTTGGAAAAGGTCTTCACCGGAAGGAGCCGCTTGTATAAGGTGTCTTTATCTTTAGTTTTTGCTCTGTGGGGGTTTGTCCTCCTTATCAGCTTATGGTTTAGTCGTGGCGACGGACACAGAGATGGATCCACAGCATCTCCAGTTGGATTATCAACTTGGAATGAAACTAAGCTGGACCGTGATGAACACACTGATTCTGTAGAACTACAAAAGCAAGCAGATTTAGTCTACTCTTCTGAAGGTGTGTGCACAAATGGTGTTGAAACTAGTAGTTTAAATGGTGAACTACTTAGTGAAGGAAATAAACATCAGGCCTCAGCAGAAGGAAGTGCAATTTATAACTCGGCTGAAGCTGAGCAACCTGAATTGGAGAAATCTAGTTCAGGCGTGAAACATGAAATTGATGCTTCAAAAAATGGCCGGCTGCCTCGTGCTGTGCCTCTTGGTCTGGATGAATTCAAGAGCAAAACATTTAGTTCAAAAATTAAATCTGTAATTGGTCAGGCTGGAGGCATAAAACATAGAGTGGAGCCTGGTGGTGCAGAGTACAATTATGCTTCAGCTGCAAAGGGAGCCAAGGTCTTGGCTTTTAACAAGGAAGCCAAGGGTGCCTCTAATATCATAAGCAGAGACAAGGACAAGTACCTTCGCAATCCATGTTCTGCAGAAGAAAAGTTTGTTGATATAGAACTTTCAGAAGAAACATTGGTAGACACAATCAAAATAGGTAATCTTGAACACTATTCATCTAATTTAAGGGATTTTGAGCTGCTTGGCAGTTTGGTTTATCCAACAGATGAATGGGTTAAGCTTGGGAATTTCACTGCAGCAAATATAAAGCTTGCGCAAAGATTTGATCTTGAGGAACCTAAGTGGGTGAGATATATAAGGTTAAAGCTTTTAAACCATCATGGTTCAGAGTTCTATTGCACAGTCAGTGTTATTGAAATTTATGGAGTTGATGCTGTTGAGCGAATGCTTGAGGATTTAATTTCTGTTGAAAGCGGCACATATGTTTCTGATGGAGTAACTGTTGACCAGAAACCTATGACCTCCCACCCAGATTCCCCTGAGGGTGATGATTTTTTTAATATTGATAAAGAAATGGAACCTCAAGCTGCAGTTGAAAACTCTAATGTGAACAATGAGATAATCAAAAGTGATGTGCCTGATCCTATCAAAGAAGTTCTTCATCAACAAGGTAGCAGGATGCCTGGGGATACTGTTCTTAAGATACTAATGCAGAAAGTCCGTTCATTGGATTTTAGTTTATCACTACTTGAGCGATACCTTGAGGAATCAAATTCAAGATACGGCAGCATTTTCAAAGAATTCGACACAGATATGGAAGAGAAAGAGTTAGATTTACAGAAGATCAAAGAAAACATGAGGAATCTCCTAGAAAGCCAGGAAGTAATTGTATGTAAAACTCTTAACTTACACCTTTAG

Protein Analysis

496

Amino Acids

55.49

Weight (kDa)

5.28

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 211 - 333 4.8e-32 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
Acc16I TGCGCA 1 cut(s) 885
AccB1I GGYRCC 1 cut(s) 647
AccI GTMKAC 2 cut(s) 267, 750
AccII CGCG 1 cut(s) 13
AciI CCGC 2 cut(s) 67, 1044
AclWI GGATC 3 cut(s) 159, 172, 1205
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 5 cut(s) 494, 856, 987, 1342, 1370
AcuI CTGAAG 3 cut(s) 297, 408, 582
AfaI GTAC 2 cut(s) 587, 683
AfiI CCNNNNNNNGG 1 cut(s) 1297
AflII CTTAAG 1 cut(s) 1268
AhlI ACTAGT 1 cut(s) 305
AjnI CCWGG 3 cut(s) 571, 1252, 1446
Alw21I GWGCWC 2 cut(s) 22, 290
Alw26I GTCTC 1 cut(s) 663
Alw44I GTGCAC 1 cut(s) 286
AlwI GGATC 3 cut(s) 159, 172, 1205
AoxI GGCC 2 cut(s) 351, 457
ApaLI GTGCAC 1 cut(s) 286
ApeKI GCWGC 6 cut(s) 463, 602, 808, 866, 1157, 1356
ApoI RAATTY 5 cut(s) 494, 856, 987, 1342, 1370
Asp700I GAANNNNTTC 1 cut(s) 1227
AspLEI GCGC 1 cut(s) 886
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 4 cut(s) 46, 332, 928, 1127
AvaII GGWCC 1 cut(s) 214
AxyI CCTNAGG 1 cut(s) 1110
BaeGI GKGCMC 1 cut(s) 290
BamHI GGATCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 1 cut(s) 22
BauI CACGAG 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 43
Bbv12I GWGCWC 2 cut(s) 22, 290
BbvCI CCTCAGC 1 cut(s) 355
BbvI GCAGC 6 cut(s) 450, 589, 795, 878, 1144, 1368
BccI CCATC 3 cut(s) 155, 954, 1055
BceAI ACGGC 1 cut(s) 1369
BciT130I CCWGG 3 cut(s) 573, 1254, 1448
BciVI GTATCC 1 cut(s) 1252
BcoDI GTCTC 1 cut(s) 663
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 3 cut(s) 306, 417, 1439
BfmI CTRYAG 4 cut(s) 237, 702, 864, 1158
BfrI CTTAAG 1 cut(s) 1268
BfuI GTATCC 1 cut(s) 1252
BisI GCNGC 8 cut(s) 67, 464, 603, 809, 867, 1045, 1158, 1357
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 8 cut(s) 68, 465, 604, 810, 868, 1046, 1159, 1358
Bme1390I CCNGG 3 cut(s) 573, 1254, 1448
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 906, 1149
BmrFI CCNGG 3 cut(s) 573, 1254, 1448
BmsI GCATC 4 cut(s) 182, 433, 991, 1239
BpiI GAAGAC 1 cut(s) 43
BpmI CTGGAG 2 cut(s) 162, 567
Bpu10I CCTNAGC 1 cut(s) 355
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 5 cut(s) 920, 1040, 1137, 1340, 1352
BsaJI CCNNGG 3 cut(s) 615, 642, 1253
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 6 cut(s) 160, 190, 603, 633, 1056, 1086
Bsc4I CCNNNNNNNGG 1 cut(s) 1297
Bse118I RCCGGY 1 cut(s) 459
Bse1I ACTGG 1 cut(s) 179
Bse21I CCTNAGG 1 cut(s) 1110
BseBI CCWGG 3 cut(s) 573, 1254, 1448
BseDI CCNNGG 3 cut(s) 615, 642, 1253
BseGI GGATG 2 cut(s) 496, 1254
BseLI CCNNNNNNNGG 1 cut(s) 1297
BseMII CTCAG 3 cut(s) 369, 384, 1101
BseNI ACTGG 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 6 cut(s) 450, 589, 795, 878, 1144, 1368
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 13
BshFI GGCC 2 cut(s) 353, 459
BshNI GGYRCC 1 cut(s) 647
BsiHKAI GWGCWC 2 cut(s) 22, 290
BsiSI CCGG 2 cut(s) 57, 460
BslI CCNNNNNNNGG 1 cut(s) 1297
BsmAI GTCTC 1 cut(s) 663
BsmI GAATGC 1 cut(s) 1020
BsnI GGCC 2 cut(s) 353, 459
Bsp1286I GDGCHC 2 cut(s) 22, 290
Bsp143I GATC 4 cut(s) 164, 895, 1210, 1413
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 2 cut(s) 67, 1044
BspANI GGCC 2 cut(s) 353, 459
BspCNI CTCAG 3 cut(s) 368, 385, 1102
BspFNI CGCG 1 cut(s) 13
BspLI GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 906, 1149
BspMAI CTGCAG 3 cut(s) 706, 868, 1162
BspPI GGATC 3 cut(s) 159, 172, 1205
BspQI GCTCTTC 2 cut(s) 27, 28
BspT107I GGYRCC 1 cut(s) 647
BspTI CTTAAG 1 cut(s) 1268
BsrFI RCCGGY 1 cut(s) 459
BsrI ACTGG 1 cut(s) 179
BssAI RCCGGY 1 cut(s) 459
BssECI CCNNGG 3 cut(s) 615, 642, 1253
BssMI GATC 4 cut(s) 164, 895, 1210, 1413
BssSI CACGAG 1 cut(s) 468
BssT1I CCWWGG 2 cut(s) 615, 642
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 3 cut(s) 573, 1254, 1448
Bst4CI ACNGT 4 cut(s) 218, 973, 1072, 1264
Bst6I CTCTTC 4 cut(s) 27, 28, 277, 1383
BstAFI CTTAAG 1 cut(s) 1268
BstC8I GCNNGC 3 cut(s) 255, 461, 882
BstDEI CTNAG 6 cut(s) 207, 329, 355, 393, 909, 1110
BstF5I GGATG 2 cut(s) 496, 1254
BstFNI CGCG 1 cut(s) 13
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 4 cut(s) 167, 898, 1213, 1416
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 4 cut(s) 164, 895, 1210, 1413
BstMWI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
BstNI CCWGG 3 cut(s) 573, 1254, 1448
BstSCI CCNGG 3 cut(s) 571, 1252, 1446
BstSFI CTRYAG 4 cut(s) 237, 702, 864, 1158
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 6 cut(s) 450, 589, 795, 878, 1144, 1368
BstV2I GAAGAC 1 cut(s) 43
BstX2I RGATCY 1 cut(s) 164
BstXI CCANNNNNNTGG 1 cut(s) 622
BstYI RGATCY 1 cut(s) 164
Bsu36I CCTNAGG 1 cut(s) 1110
BsuI GTATCC 1 cut(s) 1252
BsuRI GGCC 2 cut(s) 353, 459
BtsCI GGATG 2 cut(s) 496, 1254
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 3 cut(s) 228, 861, 982
Cac8I GCNNGC 3 cut(s) 255, 461, 882
CfoI GCGC 1 cut(s) 886
Cfr10I RCCGGY 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 2 cut(s) 586, 682
CviAII CATG 4 cut(s) 434, 696, 950, 1426
CviQI GTAC 2 cut(s) 586, 682
DdeI CTNAG 6 cut(s) 207, 329, 355, 393, 909, 1110
DpnI GATC 4 cut(s) 166, 897, 1212, 1415
DpnII GATC 4 cut(s) 164, 895, 1210, 1413
DraI TTTAAA 2 cut(s) 315, 942
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 4 cut(s) 27, 28, 277, 1383
EarI CTCTTC 4 cut(s) 27, 28, 277, 1383
Ecl136II GAGCTC 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 615, 642
Eco147I AGGCCT 1 cut(s) 353
Eco24I GRGCYC 1 cut(s) 22
Eco47I GGWCC 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 20
Eco57I CTGAAG 3 cut(s) 297, 408, 582
Eco81I CCTNAGG 1 cut(s) 1110
EcoICRI GAGCTC 1 cut(s) 20
EcoRI GAATTC 2 cut(s) 494, 1370
EcoRII CCWGG 3 cut(s) 571, 1252, 1446
EcoT14I CCWWGG 2 cut(s) 615, 642
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 2 cut(s) 615, 642
FaeI CATG 4 cut(s) 437, 699, 953, 1429
FalI AAGNNNNNCTT 2 cut(s) 1212, 1244
FatI CATG 4 cut(s) 433, 695, 949, 1425
FauNDI CATATG 1 cut(s) 1051
FblI GTMKAC 2 cut(s) 267, 750
Fnu4HI GCNGC 8 cut(s) 67, 464, 603, 809, 867, 1045, 1158, 1357
FokI GGATG 2 cut(s) 503, 1261
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 8 cut(s) 67, 464, 603, 809, 867, 1045, 1158, 1357
FspBI CTAG 3 cut(s) 306, 417, 1439
FspI TGCGCA 1 cut(s) 885
GlaI GCGC 1 cut(s) 885
GluI GCNGC 8 cut(s) 67, 464, 603, 809, 867, 1045, 1158, 1357
GsuI CTGGAG 2 cut(s) 162, 567
HaeIII GGCC 2 cut(s) 353, 459
HapII CCGG 2 cut(s) 57, 460
HhaI GCGC 1 cut(s) 886
Hin1II CATG 4 cut(s) 437, 699, 953, 1429
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HindIII AAGCTT 3 cut(s) 848, 878, 935
HinfI GANTC 4 cut(s) 233, 1103, 1337, 1432
HpaII CCGG 2 cut(s) 57, 460
HphI GGTGA 4 cut(s) 46, 332, 928, 1127
Hpy166II GTNNAC 6 cut(s) 268, 288, 323, 751, 1075, 1180
Hpy188I TCNGA 4 cut(s) 277, 736, 958, 1060
Hpy188III TCNNGA 5 cut(s) 488, 499, 773, 899, 1347
Hpy8I GTNNAC 6 cut(s) 268, 288, 323, 751, 1075, 1180
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 54, 272, 329, 356, 695
HpyCH4III ACNGT 4 cut(s) 218, 973, 1072, 1264
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
HpyF3I CTNAG 6 cut(s) 207, 329, 355, 393, 909, 1110
Hsp92II CATG 4 cut(s) 437, 699, 953, 1429
HspAI GCGC 1 cut(s) 884
KroI GCCGGC 1 cut(s) 459
KroNI GCCGGC 1 cut(s) 461
Kzo9I GATC 4 cut(s) 164, 895, 1210, 1413
LguI GCTCTTC 2 cut(s) 27, 28
LmnI GCTCC 3 cut(s) 63, 568, 611
Lsp1109I GCAGC 6 cut(s) 450, 589, 795, 878, 1144, 1368
LweI GCATC 4 cut(s) 182, 433, 991, 1239
MaeI CTAG 3 cut(s) 306, 417, 1439
MaeIII GTNAC 1 cut(s) 1066
MalI GATC 4 cut(s) 166, 897, 1212, 1415
MboI GATC 4 cut(s) 164, 895, 1210, 1413
MboII GAAGA 9 cut(s) 14, 43, 45, 264, 719, 749, 1220, 1400, 1423
MflI RGATCY 1 cut(s) 164
MhlI GDGCHC 2 cut(s) 22, 290
MmeI TCCRAC 2 cut(s) 163, 854
MroNI GCCGGC 1 cut(s) 459
MroXI GAANNNNTTC 1 cut(s) 1227
MslI CAYNNNNRTG 2 cut(s) 159, 1382
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 1 cut(s) 1268
MspI CCGG 2 cut(s) 57, 460
MspR9I CCNGG 3 cut(s) 573, 1254, 1448
Mva1269I GAATGC 1 cut(s) 1020
MvaI CCWGG 3 cut(s) 573, 1254, 1448
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 4 cut(s) 10, 602, 611, 647
NaeI GCCGGC 1 cut(s) 461
NdeI CATATG 1 cut(s) 1051
NdeII GATC 4 cut(s) 164, 895, 1210, 1413
NgoMIV GCCGGC 1 cut(s) 459
NlaIII CATG 4 cut(s) 437, 699, 953, 1429
NlaIV GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 906, 1149
NmeAIII GCCGAG 1 cut(s) 362
NsbI TGCGCA 1 cut(s) 885
PceI AGGCCT 1 cut(s) 353
PciSI GCTCTTC 2 cut(s) 27, 28
PctI GAATGC 1 cut(s) 1020
PdiI GCCGGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 1227
PfeI GAWTC 4 cut(s) 233, 1103, 1337, 1432
PkrI GCNGC 8 cut(s) 68, 465, 604, 810, 868, 1046, 1159, 1358
PsiI TTATAA 1 cut(s) 378
Psp124BI GAGCTC 1 cut(s) 22
Psp6I CCWGG 3 cut(s) 571, 1252, 1446
PspGI CCWGG 3 cut(s) 571, 1252, 1446
PspN4I GGNNCC 7 cut(s) 65, 166, 570, 613, 649, 906, 1149
PspPI GGNCC 1 cut(s) 214
PstI CTGCAG 3 cut(s) 706, 868, 1162
PsuI RGATCY 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 2 cut(s) 587, 683
RsaNI GTAC 2 cut(s) 586, 682
RseI CAYNNNNRTG 2 cut(s) 159, 1382
SacI GAGCTC 1 cut(s) 22
SapI GCTCTTC 2 cut(s) 27, 28
SatI GCNGC 8 cut(s) 67, 464, 603, 809, 867, 1045, 1158, 1357
Sau3AI GATC 4 cut(s) 164, 895, 1210, 1413
Sau96I GGNCC 1 cut(s) 214
ScrFI CCNGG 3 cut(s) 573, 1254, 1448
SduI GDGCHC 2 cut(s) 22, 290
SfaNI GCATC 4 cut(s) 182, 433, 991, 1239
SfcI CTRYAG 4 cut(s) 237, 702, 864, 1158
SinI GGWCC 1 cut(s) 214
SmiMI CAYNNNNRTG 2 cut(s) 159, 1382
SmlI CTYRAG 6 cut(s) 899, 1019, 1152, 1268, 1319, 1331
SmoI CTYRAG 6 cut(s) 899, 1019, 1152, 1268, 1319, 1331
SpeI ACTAGT 1 cut(s) 305
SseBI AGGCCT 1 cut(s) 353
SsiI CCGC 2 cut(s) 67, 1044
SspI AATATT 1 cut(s) 1132
SspMI CTAG 3 cut(s) 306, 417, 1439
SstI GAGCTC 1 cut(s) 22
StuI AGGCCT 1 cut(s) 353
StyD4I CCNGG 3 cut(s) 571, 1252, 1446
StyI CCWWGG 2 cut(s) 615, 642
TaaI ACNGT 4 cut(s) 218, 973, 1072, 1264
TaqI TCGA 1 cut(s) 1374
TatI WGTACW 1 cut(s) 585
TauI GCSGC 2 cut(s) 69, 1047
TfiI GAWTC 4 cut(s) 233, 1103, 1337, 1432
TscAI CASTG 3 cut(s) 235, 868, 982
TseI GCWGC 6 cut(s) 463, 602, 808, 866, 1157, 1356
TspDTI ATGAA 8 cut(s) 216, 237, 450, 507, 774, 852, 1220, 1284
TspGWI ACGGA 2 cut(s) 165, 1280
TspRI CASTG 3 cut(s) 235, 868, 982
Vha464I CTTAAG 1 cut(s) 1268
VneI GTGCAC 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 214
XapI RAATTY 5 cut(s) 494, 856, 987, 1342, 1370
XcmI CCANNNNNNNNNTGG 1 cut(s) 837
XmiI GTMKAC 2 cut(s) 267, 750
XmnI GAANNNNTTC 1 cut(s) 1227
XspI CTAG 3 cut(s) 306, 417, 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.