MD05G1016700.v1.1

GDP-mannose transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008023
Physical Location & Seq
Reverse (-)
2932359 .. 2937878
5520 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1016700.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGAGTTCTTTTGACAAGAATGAAACCGATTTGGAAGGTGGAAAATTGCAAAAAGATGGAGATAAATCAACAAGGGGTAATAAGGTGGTTAAGATACAGAATAAGGCATTGTTGTCTGGCCTTGCATATTGCCTGTCGTCCTGCAGCATGATATTAGTTAACAAATTCGTCCTTTCGAGCTACAACTTTGATGCTGGAATTTCTCTGATGCTGTACCAGAACCTAGTCTCGGTTATAATCGTTTCTGTGTTGACTTTTACCGGCATCATATCAACGGAGCCACTAACATGGAGATTGGTTAAGGTCTGGTTGCCTGTGAATGTTATATTCGTTGGGATGCTCATTACAAGCATGTTTAGCTTGAAGTACATCAATGTAGCGATGGTCACAGTCCTAAAGAATGTTACCAACGTGATAACTGCTGTTGGTGAAATGTATCTATTCCAGAAGCACCATGACAGCAGAGTATGGGCAGCGCTTTTCCTAATGATAATTTCAGCAATTTCTGGAGGAGTCACTGATTTATCTTTCCATGCCGTTGGCTATGCATGGCAATTTCTGAACTGTATCTTGACTGCATCATATTCTCTTACTCTACGTAGAGTCATGGATACAGCTAAGCTGGTCACGAAATCTGGAAAGCTGAATGAGTTTTCAATGGTCTTGCTAAATAACACACTTTCTCTACCTTTGGGGATTCTGCTTATTTTTGTTTTCAATGAAGTGGATTATCTCTTTACAACACCACTTTTGAGGTCTCCAACCTTCTGGTTGGTGATGACTTTAAGTGGATTTTTGGGCCTTGCAATCAGCTTCACATCTATGTGGTTTCTTCATCAAACAGGTGCCACCACATACAGCCTTGTTGGGTCGCTGAATAAGATCCCTCTATCTGTTGCCGGTATTCTACTTTTCAAAGCGCCGACCAGTTTAGAGAACTCTGCTAGCATTTTCTTTGGTCTCGTGGCTGGAGTATTTTTCGCTAGAGCCAAAATGCGGGAGAGATCTTAA

Protein Analysis

337

Amino Acids

37.12

Weight (kDa)

9.52

Isoelectric Point (pI)

24.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 38 - 326 1.1e-11 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 845
AciI CCGC 1 cut(s) 997
AclWI GGATC 1 cut(s) 877
AcsI RAATTY 2 cut(s) 164, 198
AfaI GTAC 2 cut(s) 215, 368
AfeI AGCGCT 1 cut(s) 477
AfiI CCNNNNNNNGG 2 cut(s) 229, 996
AgsI TTSAA 4 cut(s) 364, 657, 718, 916
AluBI AGCT 6 cut(s) 180, 360, 617, 622, 643, 813
AluI AGCT 6 cut(s) 180, 360, 617, 622, 643, 813
Alw26I GTCTC 3 cut(s) 232, 762, 965
AlwI GGATC 1 cut(s) 877
Aor51HI AGCGCT 1 cut(s) 477
AoxI GGCC 2 cut(s) 118, 799
ApeKI GCWGC 2 cut(s) 144, 473
ApoI RAATTY 2 cut(s) 164, 198
AspLEI GCGC 2 cut(s) 478, 922
AspS9I GGNCC 1 cut(s) 799
AsuHPI GGTGA 2 cut(s) 440, 787
AsuNHI GCTAGC 1 cut(s) 944
BanI GGYRCC 1 cut(s) 845
BauI CACGAG 1 cut(s) 962
BbvI GCAGC 2 cut(s) 156, 485
BccI CCATC 2 cut(s) 50, 376
BceAI ACGGC 1 cut(s) 521
BciVI GTATCC 1 cut(s) 604
BcoDI GTCTC 3 cut(s) 232, 762, 965
BfaI CTAG 3 cut(s) 224, 945, 984
BfmI CTRYAG 1 cut(s) 142
BfoI RGCGCY 2 cut(s) 479, 923
BfuI GTATCC 1 cut(s) 604
BglII AGATCT 1 cut(s) 1004
BisI GCNGC 2 cut(s) 145, 474
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 2 cut(s) 146, 475
BmgT120I GGNCC 1 cut(s) 799
BmiI GGNNCC 2 cut(s) 279, 847
BmsI GCATC 5 cut(s) 181, 198, 273, 327, 587
BmtI GCTAGC 1 cut(s) 948
BpmI CTGGAG 2 cut(s) 528, 990
Bpu1102I GCTNAGC 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 599
BsaI GGTCTC 2 cut(s) 762, 965
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 996
Bse118I RCCGGY 2 cut(s) 260, 899
Bse1I ACTGG 1 cut(s) 927
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 229, 996
BseNI ACTGG 1 cut(s) 927
BseRI GAGGAG 1 cut(s) 525
BseXI GCAGC 2 cut(s) 156, 485
BshFI GGCC 2 cut(s) 120, 801
BshNI GGYRCC 1 cut(s) 845
BsiSI CCGG 2 cut(s) 261, 900
BslI CCNNNNNNNGG 2 cut(s) 229, 996
BsmAI GTCTC 3 cut(s) 232, 762, 965
BsnI GGCC 2 cut(s) 120, 801
Bso31I GGTCTC 2 cut(s) 762, 965
Bsp143I GATC 2 cut(s) 882, 1004
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 1 cut(s) 997
BspANI GGCC 2 cut(s) 120, 801
BspLI GGNNCC 2 cut(s) 279, 847
BspMAI CTGCAG 1 cut(s) 146
BspOI GCTAGC 1 cut(s) 948
BspPI GGATC 1 cut(s) 877
BspT107I GGYRCC 1 cut(s) 845
BspTNI GGTCTC 2 cut(s) 762, 965
BsrFI RCCGGY 2 cut(s) 260, 899
BsrI ACTGG 1 cut(s) 927
BssAI RCCGGY 2 cut(s) 260, 899
BssMI GATC 2 cut(s) 882, 1004
BssSI CACGAG 1 cut(s) 962
Bst2BI CACGAG 1 cut(s) 962
Bst4CI ACNGT 2 cut(s) 391, 566
BstBAI YACGTR 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 946
BstDEI CTNAG 1 cut(s) 618
BstF5I GGATG 1 cut(s) 342
BstH2I RGCGCY 2 cut(s) 479, 923
BstHHI GCGC 2 cut(s) 478, 922
BstKTI GATC 2 cut(s) 885, 1007
BstMAI GTCTC 3 cut(s) 232, 762, 965
BstMBI GATC 2 cut(s) 882, 1004
BstMWI GCNNNNNNNGC 1 cut(s) 357
BstNSI RCATGY 1 cut(s) 355
BstSFI CTRYAG 1 cut(s) 142
BstSNI TACGTA 1 cut(s) 599
BstV1I GCAGC 2 cut(s) 156, 485
BstX2I RGATCY 2 cut(s) 882, 1004
BstXI CCANNNNNNTGG 3 cut(s) 288, 539, 768
BstYI RGATCY 2 cut(s) 882, 1004
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 2 cut(s) 120, 801
BtgZI GCGATG 1 cut(s) 395
BtsCI GGATG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 516
Cac8I GCNNGC 1 cut(s) 946
CfoI GCGC 2 cut(s) 478, 922
Cfr10I RCCGGY 2 cut(s) 260, 899
Cfr13I GGNCC 1 cut(s) 799
Csp6I GTAC 2 cut(s) 214, 367
CviAII CATG 7 cut(s) 148, 288, 352, 455, 533, 549, 607
CviQI GTAC 2 cut(s) 214, 367
DdeI CTNAG 1 cut(s) 618
DpnI GATC 2 cut(s) 884, 1006
DpnII GATC 2 cut(s) 882, 1004
Eco105I TACGTA 1 cut(s) 599
Eco31I GGTCTC 2 cut(s) 762, 965
Eco47III AGCGCT 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 550
FaeI CATG 7 cut(s) 151, 291, 355, 458, 536, 552, 610
FatI CATG 7 cut(s) 147, 287, 351, 454, 532, 548, 606
FauI CCCGC 1 cut(s) 990
Fnu4HI GCNGC 2 cut(s) 145, 474
FokI GGATG 1 cut(s) 349
Fsp4HI GCNGC 2 cut(s) 145, 474
FspBI CTAG 3 cut(s) 224, 945, 984
GlaI GCGC 2 cut(s) 477, 921
GluI GCNGC 2 cut(s) 145, 474
GsuI CTGGAG 2 cut(s) 528, 990
HaeII RGCGCY 2 cut(s) 479, 923
HaeIII GGCC 2 cut(s) 120, 801
HapII CCGG 2 cut(s) 261, 900
HhaI GCGC 2 cut(s) 478, 922
Hin1II CATG 7 cut(s) 151, 291, 355, 458, 536, 552, 610
Hin6I GCGC 2 cut(s) 476, 920
HinP1I GCGC 2 cut(s) 476, 920
HincII GTYRAC 2 cut(s) 160, 252
HindII GTYRAC 2 cut(s) 160, 252
HinfI GANTC 3 cut(s) 513, 603, 697
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 2 cut(s) 261, 900
HphI GGTGA 2 cut(s) 440, 787
Hpy166II GTNNAC 2 cut(s) 160, 252
Hpy188I TCNGA 2 cut(s) 207, 561
Hpy188III TCNNGA 5 cut(s) 445, 507, 571, 628, 636
Hpy8I GTNNAC 2 cut(s) 160, 252
HpyAV CCTTC 2 cut(s) 29, 775
HpyCH4III ACNGT 2 cut(s) 391, 566
HpyCH4IV ACGT 2 cut(s) 411, 598
HpyCH4V TGCA 6 cut(s) 49, 125, 144, 548, 578, 806
HpyF10VI GCNNNNNNNGC 1 cut(s) 357
HpyF3I CTNAG 1 cut(s) 618
HpySE526I ACGT 2 cut(s) 411, 598
Hsp92II CATG 7 cut(s) 151, 291, 355, 458, 536, 552, 610
HspAI GCGC 2 cut(s) 476, 920
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 2 cut(s) 882, 1004
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 2 cut(s) 156, 485
LweI GCATC 5 cut(s) 181, 198, 273, 327, 587
MaeI CTAG 3 cut(s) 224, 945, 984
MaeII ACGT 2 cut(s) 411, 598
MaeIII GTNAC 4 cut(s) 385, 403, 514, 625
MalI GATC 2 cut(s) 884, 1006
MboI GATC 2 cut(s) 882, 1004
MboII GAAGA 1 cut(s) 824
MflI RGATCY 2 cut(s) 882, 1004
MluCI AATT 6 cut(s) 44, 164, 198, 492, 501, 554
MlyI GAGTC 2 cut(s) 522, 612
MmeI TCCRAC 1 cut(s) 785
MnlI CCTC 3 cut(s) 503, 747, 897
Mph1103I ATGCAT 1 cut(s) 550
MseI TTAA 5 cut(s) 90, 159, 300, 785, 1009
MslI CAYNNNNRTG 3 cut(s) 286, 821, 823
MspI CCGG 2 cut(s) 261, 900
MwoI GCNNNNNNNGC 1 cut(s) 357
NdeII GATC 2 cut(s) 882, 1004
NheI GCTAGC 1 cut(s) 944
NlaIII CATG 7 cut(s) 151, 291, 355, 458, 536, 552, 610
NlaIV GGNNCC 2 cut(s) 279, 847
NmuCI GTSAC 3 cut(s) 385, 514, 625
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 1 cut(s) 355
PfeI GAWTC 1 cut(s) 697
PkrI GCNGC 2 cut(s) 146, 475
PleI GAGTC 2 cut(s) 521, 611
PpsI GAGTC 2 cut(s) 521, 611
Ppu21I YACGTR 1 cut(s) 599
PsiI TTATAA 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 279, 847
PspPI GGNCC 1 cut(s) 799
PstI CTGCAG 1 cut(s) 146
PsuI RGATCY 2 cut(s) 882, 1004
RsaI GTAC 2 cut(s) 215, 368
RsaNI GTAC 2 cut(s) 214, 367
RseI CAYNNNNRTG 3 cut(s) 286, 821, 823
SaqAI TTAA 5 cut(s) 90, 159, 300, 785, 1009
SatI GCNGC 2 cut(s) 145, 474
Sau3AI GATC 2 cut(s) 882, 1004
Sau96I GGNCC 1 cut(s) 799
SchI GAGTC 2 cut(s) 522, 612
SfaNI GCATC 5 cut(s) 181, 198, 273, 327, 587
SfcI CTRYAG 1 cut(s) 142
SmiMI CAYNNNNRTG 3 cut(s) 286, 821, 823
SnaBI TACGTA 1 cut(s) 599
Sse9I AATT 6 cut(s) 44, 164, 198, 492, 501, 554
SsiI CCGC 1 cut(s) 997
SspMI CTAG 3 cut(s) 224, 945, 984
TaaI ACNGT 2 cut(s) 391, 566
TaiI ACGT 2 cut(s) 414, 601
TaqI TCGA 1 cut(s) 176
TasI AATT 6 cut(s) 44, 164, 198, 492, 501, 554
TatI WGTACW 1 cut(s) 366
TfiI GAWTC 1 cut(s) 697
Tru1I TTAA 5 cut(s) 90, 159, 300, 785, 1009
Tru9I TTAA 5 cut(s) 90, 159, 300, 785, 1009
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 3 cut(s) 385, 514, 625
TseI GCWGC 2 cut(s) 144, 473
Tsp45I GTSAC 3 cut(s) 385, 514, 625
TspDTI ATGAA 3 cut(s) 36, 735, 824
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 1 cut(s) 523
XapI RAATTY 2 cut(s) 164, 198
XceI RCATGY 1 cut(s) 355
XspI CTAG 3 cut(s) 224, 945, 984
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.