MD05G1034600.v1.1

Ribosomal protein S1-like RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013437
Physical Location & Seq
Forward (+)
5604641 .. 5618039
13399 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1034600.v1.1.491

Sequence Viewer

Length: 1569 bp
ATGCTACTCCTTGCTCACCCCTGTAAGTCTTTCTCCTTCCCCGACTCGTCACTTCATCTCGACAATTTCAACTCACAAACCTCAGCATCTGCAAAACCTTCAAGTTATAGCTCCTTTTCCCCAATTGGGTTGTCGAAAAGTCTGAAACTTTGTAGGAATTTTCCCGTTTTGGGAAGTACCCGGATTACGTTTTGCTCTAGGAGTGAGGTGTATGATATTTTTCCAAGTACCCATTTGGAGAACAAGTCTGAAGGGGATGGGGTTGGAGAGAATGAAGAGCTTGAGCTGCTGGATAAGCCTTCCCATGTACCTATAAGTAATGGGTCTGCTTTGGAAGTGGATAAAGATTCCGAAAAACCGGATGAAGAATCGGAAATGATTGATAAAGACGAGGCATTGGCGCCTTTCTTGAAGTTTTTCGCGCCGAGAGATAGTGCGGATGGAGAGGGAGTGGAAGGAAAGGGAGGGGAGGTTGGAGTTTTCGAGGAAGAAAGTGAATTGGATGGTGATAATGAGGAGGCTGAGAAGGTTAATGTGGAATACTATGAACCGAAACCGGGTGATTTTGTGGTTGGAATTGTTGTTTCAGGCAATGAGAATAAACTTGATGTGAATGTCGGAGCAGACTTGTTGGGTACAATGCTGACTAAGGAAGTGCTGCCTTTGTATGACAAAGAGATGGATTACTTGTTATGTGATACGAATTATGATGCCGAAGAGTTTATGGTTAGAGGGAAGATGGGAATTGTGAAAAATGAAGCTGTGATGGGGGGACCTGTGCTGGGAGGGCCCGTTGTTGAAATCGGAACTGTTTTGTTTGCTGAGGTTCTTGGAAGAACGCTCAGCGGTAGGCCTTTGCTTTCAACAAGACGGCTCTTCAGGCGAATTGCTTGGCACCGAGTGAGGCAGATAAAGCAACTCAATGAACCTATTGAAGTTAGAATTACAGAATGGAATACTGGAGGCCTTCTGACAAGAATTGAGGGTCTGCGAGCTTTCCTTCCAAAAACTGAGTTAATGAGTAAAGTGAACAACTTCACTGAATTGAAAGAGAATGTGGGTTGCCGGATGCATGTGCAGATTACTCGAATAGACGAAACTAAGAATGACTTAGTTTTGAGTGAGAAAGAAGCTTGGGAACTGTTATACCTTAAGGAGGGAACACTTTTAGAAGGAACTGTCAAGAAAATCTTTCCATATGGAGCCCAAGTGAGGATAGGAGAAACTAACAGGAGTGGCTTGTTGCATATATCAAACATCACTCGTGCCCGAATTACCTCTGTCAGTGACATACTTAAGGTTAATGAGAAGCTCAAAGTTCTGGTTGTGAAGTCGATGTTTCCTGACAAAATATCTCTCAGCATTGCTGACCTTGAAAGTGAGCCTGGCCTCTTTTTGTCGAACAGAGAGAAATTATTTTCGGAGGCTCAAATGATGGCAAAGAAGTATAGGCAAAAGCTGCCTGCTGTTCTTGCCAACCGCAAGTCAGAATCCCCTGAAAACGATGCTCTGCCTTTTGACAAATTAAGCATGTATGCAAATTGGAAGTGGTTCAAATTCGAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
S1

Protein Analysis

523

Amino Acids

58.46

Weight (kDa)

5.16

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 384 - 444 1.1e-08 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71720 AT1G71720
fragaria_vesca FvH4_2g12900
malus_domestica MD05G1034600.v1.1
prunus_persica Prupe.8G042900_v2.0.a1 Prupe.8G042900_v2.0.a1
pyrus_communis pycom05g02520
rosa_chinensis RchiOBHm_Chr6g0274001
rosa_laevigata RLG00000013585
rosa_multiflora Rmu_sc0000045.1_g000088 Rmu_sc0002247.1_g000020
rosa_roxburghii Rroxscaffold_7G00194800
rosa_rugosa Rorug06G0078200
rosa_samantha Rh6AG193800 Rh6BG197400 Rh6CG197300 Rh6DG189200
rosa_wichuraiana Rw6G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 400, 894
AccII CGCG 1 cut(s) 422
AciI CCGC 3 cut(s) 437, 846, 1480
AcsI RAATTY 2 cut(s) 157, 1556
AcuI CTGAAG 2 cut(s) 270, 862
AcyI GRCGYC 1 cut(s) 401
AdeI CACNNNGTG 1 cut(s) 901
AfaI GTAC 4 cut(s) 178, 229, 309, 637
AfiI CCNNNNNNNGG 6 cut(s) 126, 170, 557, 782, 1156, 1212
AflII CTTAAG 2 cut(s) 1151, 1295
AgsI TTSAA 9 cut(s) 70, 102, 412, 800, 864, 935, 1048, 1376, 1555
AjnI CCWGG 1 cut(s) 1384
AluBI AGCT 8 cut(s) 111, 280, 286, 761, 995, 1133, 1312, 1459
AluI AGCT 8 cut(s) 111, 280, 286, 761, 995, 1133, 1312, 1459
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 4 cut(s) 788, 851, 964, 1387
ApaI GGGCCC 1 cut(s) 792
ApeKI GCWGC 3 cut(s) 286, 658, 1459
ApoI RAATTY 2 cut(s) 157, 1556
Asp700I GAANNNNTTC 3 cut(s) 416, 1034, 1550
AspLEI GCGC 2 cut(s) 403, 424
AspS9I GGNCC 3 cut(s) 773, 788, 789
AsuC2I CCSGG 2 cut(s) 181, 558
AsuHPI GGTGA 3 cut(s) 8, 518, 572
AsuII TTCGAA 1 cut(s) 1560
AvaII GGWCC 1 cut(s) 773
BaeGI GKGCMC 2 cut(s) 792, 1270
BanI GGYRCC 2 cut(s) 400, 894
BanII GRGCYC 2 cut(s) 792, 1207
BauI CACGAG 1 cut(s) 1263
BbvCI CCTCAGC 2 cut(s) 82, 822
BbvI GCAGC 3 cut(s) 273, 645, 1446
BccI CCATC 7 cut(s) 251, 434, 497, 673, 733, 760, 1429
BceAI ACGGC 1 cut(s) 887
BcgI CGANNNNNNTGC 2 cut(s) 1067, 1101
BciT130I CCWGG 1 cut(s) 1386
BcnI CCSGG 2 cut(s) 181, 558
BfaI CTAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 404
BfrI CTTAAG 2 cut(s) 1151, 1295
BisI GCNGC 3 cut(s) 287, 659, 1460
BlpI GCTNAGC 1 cut(s) 842
BlsI GCNGC 3 cut(s) 288, 660, 1461
Bme1390I CCNGG 3 cut(s) 181, 558, 1386
Bme18I GGWCC 1 cut(s) 773
BmgT120I GGNCC 3 cut(s) 773, 788, 789
BmiI GGNNCC 5 cut(s) 402, 774, 790, 896, 1204
BmrFI CCNGG 3 cut(s) 181, 558, 1386
BmsI GCATC 4 cut(s) 95, 700, 1059, 1495
BpmI CTGGAG 1 cut(s) 981
Bpu10I CCTNAGC 2 cut(s) 82, 822
Bpu1102I GCTNAGC 1 cut(s) 842
Bpu14I TTCGAA 1 cut(s) 1560
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 2 cut(s) 181, 558
BsaHI GRCGYC 1 cut(s) 401
BsaWI WCCGGW 1 cut(s) 358
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 170, 557, 782, 1156, 1212
Bse1I ACTGG 1 cut(s) 964
Bse3DI GCAATG 2 cut(s) 598, 1362
BseBI CCWGG 1 cut(s) 1386
BseGI GGATG 5 cut(s) 262, 367, 445, 508, 1074
BseLI CCNNNNNNNGG 6 cut(s) 126, 170, 557, 782, 1156, 1212
BseMI GCAATG 2 cut(s) 598, 1362
BseMII CTCAG 6 cut(s) 96, 513, 813, 856, 1002, 1372
BseNI ACTGG 1 cut(s) 964
BseRI GAGGAG 1 cut(s) 530
BseSI GKGCMC 2 cut(s) 792, 1270
BseXI GCAGC 3 cut(s) 273, 645, 1446
BseYI CCCAGC 1 cut(s) 781
BsgI GTGCAG 1 cut(s) 1097
Bsh1236I CGCG 1 cut(s) 422
BshFI GGCC 4 cut(s) 790, 853, 966, 1389
BshNI GGYRCC 2 cut(s) 400, 894
BsiSI CCGG 4 cut(s) 181, 359, 557, 1066
BslFI GGGAC 1 cut(s) 786
BslI CCNNNNNNNGG 6 cut(s) 126, 170, 557, 782, 1156, 1212
BsmFI GGGAC 1 cut(s) 786
BsnI GGCC 4 cut(s) 790, 853, 966, 1389
Bsp119I TTCGAA 1 cut(s) 1560
Bsp120I GGGCCC 1 cut(s) 788
Bsp1286I GDGCHC 3 cut(s) 792, 1207, 1270
Bsp1720I GCTNAGC 1 cut(s) 842
BspACI CCGC 3 cut(s) 437, 846, 1480
BspANI GGCC 4 cut(s) 790, 853, 966, 1389
BspCNI CTCAG 6 cut(s) 95, 514, 814, 855, 1003, 1371
BspFNI CGCG 1 cut(s) 422
BspLI GGNNCC 5 cut(s) 402, 774, 790, 896, 1204
BspQI GCTCTTC 2 cut(s) 270, 881
BspT104I TTCGAA 1 cut(s) 1560
BspT107I GGYRCC 2 cut(s) 400, 894
BspTI CTTAAG 2 cut(s) 1151, 1295
BsrDI GCAATG 2 cut(s) 598, 1362
BsrI ACTGG 1 cut(s) 964
BssNI GRCGYC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 1263
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 1 cut(s) 1386
Bst4CI ACNGT 3 cut(s) 811, 1143, 1180
Bst6I CTCTTC 3 cut(s) 270, 711, 881
BstACI GRCGYC 1 cut(s) 401
BstAFI CTTAAG 2 cut(s) 1151, 1295
BstAPI GCANNNNNTGC 1 cut(s) 1459
BstBI TTCGAA 1 cut(s) 1560
BstC8I GCNNGC 2 cut(s) 993, 1464
BstDEI CTNAG 9 cut(s) 82, 522, 648, 822, 842, 1011, 1101, 1111, 1358
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 5 cut(s) 262, 367, 445, 508, 1074
BstFNI CGCG 1 cut(s) 422
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 2 cut(s) 403, 424
BstMWI GCNNNNNNNGC 7 cut(s) 286, 295, 787, 880, 913, 1459, 1472
BstNI CCWGG 1 cut(s) 1386
BstNSI RCATGY 2 cut(s) 1076, 1534
BstSCI CCNGG 3 cut(s) 179, 556, 1384
BstSLI GKGCMC 2 cut(s) 792, 1270
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 3 cut(s) 273, 645, 1446
BsuRI GGCC 4 cut(s) 790, 853, 966, 1389
BtsCI GGATG 5 cut(s) 262, 367, 445, 508, 1074
BtsIMutI CAGTG 2 cut(s) 1038, 1291
Cac8I GCNNGC 2 cut(s) 993, 1464
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 2 cut(s) 403, 424
Cfr13I GGNCC 3 cut(s) 773, 788, 789
Csp6I GTAC 4 cut(s) 177, 228, 308, 636
CviAII CATG 3 cut(s) 305, 1073, 1531
CviQI GTAC 4 cut(s) 177, 228, 308, 636
DdeI CTNAG 9 cut(s) 82, 522, 648, 822, 842, 1011, 1101, 1111, 1358
DinI GGCGCC 1 cut(s) 402
DraIII CACNNNGTG 1 cut(s) 901
Eam1104I CTCTTC 3 cut(s) 270, 711, 881
EarI CTCTTC 3 cut(s) 270, 711, 881
Eco147I AGGCCT 2 cut(s) 853, 966
Eco24I GRGCYC 2 cut(s) 792, 1207
Eco47I GGWCC 1 cut(s) 773
Eco57I CTGAAG 2 cut(s) 270, 862
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoO109I RGGNCCY 2 cut(s) 773, 788
EcoRII CCWGG 1 cut(s) 1384
EcoT22I ATGCAT 1 cut(s) 1074
EcoT38I GRGCYC 2 cut(s) 792, 1207
EgeI GGCGCC 1 cut(s) 402
EheI GGCGCC 1 cut(s) 402
FaeI CATG 3 cut(s) 308, 1076, 1534
FalI AAGNNNNNCTT 4 cut(s) 1094, 1126, 1175, 1207
FaqI GGGAC 1 cut(s) 786
FatI CATG 3 cut(s) 304, 1072, 1530
FauNDI CATATG 1 cut(s) 1198
Fnu4HI GCNGC 3 cut(s) 287, 659, 1460
FokI GGATG 5 cut(s) 269, 374, 452, 515, 1081
FriOI GRGCYC 2 cut(s) 792, 1207
Fsp4HI GCNGC 3 cut(s) 287, 659, 1460
FspBI CTAG 1 cut(s) 198
GlaI GCGC 2 cut(s) 402, 423
GluI GCNGC 3 cut(s) 287, 659, 1460
GsaI CCCAGC 1 cut(s) 785
GsuI CTGGAG 1 cut(s) 981
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 4 cut(s) 790, 853, 966, 1389
HapII CCGG 4 cut(s) 181, 359, 557, 1066
HhaI GCGC 2 cut(s) 403, 424
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 3 cut(s) 308, 1076, 1534
Hin6I GCGC 2 cut(s) 401, 422
HinP1I GCGC 2 cut(s) 401, 422
HindIII AAGCTT 1 cut(s) 1131
HinfI GANTC 4 cut(s) 44, 347, 368, 1490
HpaII CCGG 4 cut(s) 181, 359, 557, 1066
HphI GGTGA 3 cut(s) 8, 518, 572
Hpy166II GTNNAC 1 cut(s) 1030
Hpy188I TCNGA 9 cut(s) 144, 250, 352, 373, 620, 806, 972, 1423, 1489
Hpy188III TCNNGA 4 cut(s) 59, 409, 1183, 1343
Hpy8I GTNNAC 1 cut(s) 1030
HpyAV CCTTC 9 cut(s) 46, 108, 245, 309, 449, 520, 977, 1010, 1166
HpyCH4III ACNGT 3 cut(s) 811, 1143, 1180
HpyCH4IV ACGT 1 cut(s) 188
HpyCH4V TGCA 5 cut(s) 92, 1072, 1078, 1246, 1538
HpyF10VI GCNNNNNNNGC 7 cut(s) 286, 295, 787, 880, 913, 1459, 1472
HpyF3I CTNAG 9 cut(s) 82, 522, 648, 822, 842, 1011, 1101, 1111, 1358
HpySE526I ACGT 1 cut(s) 188
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 3 cut(s) 308, 1076, 1534
HspAI GCGC 2 cut(s) 401, 422
KasI GGCGCC 1 cut(s) 400
LguI GCTCTTC 2 cut(s) 270, 881
LmnI GCTCC 3 cut(s) 116, 620, 1202
Lsp1109I GCAGC 3 cut(s) 273, 645, 1446
LweI GCATC 4 cut(s) 95, 700, 1059, 1495
MaeI CTAG 1 cut(s) 198
MaeII ACGT 1 cut(s) 188
MaeIII GTNAC 2 cut(s) 48, 1286
MboII GAAGA 7 cut(s) 287, 377, 500, 728, 748, 846, 868
MfeI CAATTG 1 cut(s) 123
MhlI GDGCHC 3 cut(s) 792, 1207, 1270
Mly113I GGCGCC 1 cut(s) 401
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 4 cut(s) 244, 454, 553, 598
Mph1103I ATGCAT 1 cut(s) 1074
MroXI GAANNNNTTC 3 cut(s) 416, 1034, 1550
MseI TTAA 6 cut(s) 531, 1016, 1152, 1296, 1302, 1526
MspA1I CMGCKG 1 cut(s) 846
MspCI CTTAAG 2 cut(s) 1151, 1295
MspI CCGG 4 cut(s) 181, 359, 557, 1066
MspR9I CCNGG 3 cut(s) 181, 558, 1386
MunI CAATTG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 1386
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 7 cut(s) 286, 295, 787, 880, 913, 1459, 1472
NarI GGCGCC 1 cut(s) 401
NciI CCSGG 2 cut(s) 181, 558
NdeI CATATG 1 cut(s) 1198
NlaIII CATG 3 cut(s) 308, 1076, 1534
NlaIV GGNNCC 5 cut(s) 402, 774, 790, 896, 1204
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 2 cut(s) 48, 1286
NsiI ATGCAT 1 cut(s) 1074
NspI RCATGY 2 cut(s) 1076, 1534
NspV TTCGAA 1 cut(s) 1560
PceI AGGCCT 2 cut(s) 853, 966
PciSI GCTCTTC 2 cut(s) 270, 881
PdmI GAANNNNTTC 3 cut(s) 416, 1034, 1550
PfeI GAWTC 3 cut(s) 347, 368, 1490
PkrI GCNGC 3 cut(s) 288, 660, 1461
PleI GAGTC 1 cut(s) 38
PluTI GGCGCC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 38
PpuMI RGGWCCY 1 cut(s) 773
Psp5II RGGWCCY 1 cut(s) 773
Psp6I CCWGG 1 cut(s) 1384
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 1 cut(s) 1384
PspN4I GGNNCC 5 cut(s) 402, 774, 790, 896, 1204
PspOMI GGGCCC 1 cut(s) 788
PspPI GGNCC 3 cut(s) 773, 788, 789
PspPPI RGGWCCY 1 cut(s) 773
PstNI CAGNNNCTG 1 cut(s) 89
RsaI GTAC 4 cut(s) 178, 229, 309, 637
RsaNI GTAC 4 cut(s) 177, 228, 308, 636
SapI GCTCTTC 2 cut(s) 270, 881
SaqAI TTAA 6 cut(s) 531, 1016, 1152, 1296, 1302, 1526
SatI GCNGC 3 cut(s) 287, 659, 1460
Sau96I GGNCC 3 cut(s) 773, 788, 789
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 3 cut(s) 181, 558, 1386
SduI GDGCHC 3 cut(s) 792, 1207, 1270
SfaNI GCATC 4 cut(s) 95, 700, 1059, 1495
SfoI GGCGCC 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 1560
SinI GGWCC 1 cut(s) 773
SmlI CTYRAG 3 cut(s) 281, 1151, 1295
SmoI CTYRAG 3 cut(s) 281, 1151, 1295
SseBI AGGCCT 2 cut(s) 853, 966
SsiI CCGC 3 cut(s) 437, 846, 1480
SspDI GGCGCC 1 cut(s) 400
SspMI CTAG 1 cut(s) 198
StuI AGGCCT 2 cut(s) 853, 966
StyD4I CCNGG 3 cut(s) 179, 556, 1384
TaaI ACNGT 3 cut(s) 811, 1143, 1180
TaiI ACGT 1 cut(s) 191
TaqI TCGA 7 cut(s) 60, 134, 483, 1087, 1334, 1400, 1560
TfiI GAWTC 3 cut(s) 347, 368, 1490
Tru1I TTAA 6 cut(s) 531, 1016, 1152, 1296, 1302, 1526
Tru9I TTAA 6 cut(s) 531, 1016, 1152, 1296, 1302, 1526
TscAI CASTG 2 cut(s) 1045, 1291
TseFI GTSAC 2 cut(s) 48, 1286
TseI GCWGC 3 cut(s) 286, 658, 1459
Tsp45I GTSAC 2 cut(s) 48, 1286
TspDTI ATGAA 6 cut(s) 44, 288, 378, 561, 771, 939
TspRI CASTG 2 cut(s) 1045, 1291
Vha464I CTTAAG 2 cut(s) 1151, 1295
VpaK11BI GGWCC 1 cut(s) 773
XagI CCTNNNNNAGG 1 cut(s) 1154
XapI RAATTY 2 cut(s) 157, 1556
XceI RCATGY 2 cut(s) 1076, 1534
XmnI GAANNNNTTC 3 cut(s) 416, 1034, 1550
XspI CTAG 1 cut(s) 198
Zsp2I ATGCAT 1 cut(s) 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.