Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0011651

Physical Location & Seq

Genomic Coordinates Reverse (-)

6944136 .. 6948447

Gene Structure 4312 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1041200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGTTCGATTGCACTCGCAAATCACAGTATATTGGTGGCCAAAAGGAGAAATTTGTACGGTTGGATGACTTGGGTTCTACGTATTCCATGAGTTCTGATACCGGTAAAATGAATAAATGTGGATTTAGTATCGAGTCATTCCCCTTTACTGGTGGAAAACCAAAGAGAGCACCGTCACAATCATTCAGGTATGGAATTCAACAGGGAACAGATGGACTAAAGACATTTGGTAGATCATTAAGAACTGGCGTCACCCGCGCAGTGTTCCCGGAAGATCTTAAAGTGTCTGATAAAAAGATATTTGACCCCCAAGACAAATCCCTCCTGCTATGGAACAGGCTTTTCGTCATAACTTGCATCTTTGCAATATCCATTGACCCTTTGTTCTTCTATCTTCCAATCTTCGACAATGATGAGGATTGCCTCTCTATCGATCGAAGATTAGGAACGACAACTATAACTATACGGTCATTAGTAGATGCTTTCTACCTTATTCGCATGGCTTTACAGTTTCGTACAGCTTATATTGCTCCATCATCTCGGGTTTTCGGAAGAGGCGAACTTGTGATAGATCCTCCGCAGATAGCAAAGCGATATCTACACCGTTATTTCTTAGTTGACTTGGCTTCTGTGCTCCCCTTACCCCAGATTGTGGTTTGGAGATTCTTGCATGGAAGAGAGTCAGAGGTTTTGGCTACGAAACAGGCATTGCTGTTTATTGTCTGGCTCCAGTTACTACCGAGATTTATCCGCTTATTAGCTTTAAACTCAGACCTGAAAAGGAGTGCAGGTGCTTTTGCCGAAAGTGCTTGGGCCGGGGCTGCCTACTATTTGCTTTGGTTTATGCTTGCCGCTCAAGTCACCGGGGCTGCATGGTACTTATTAGCTGTGGAGCGCTATGATGGATGCTGGCGGGGAGCTTGTAGAAATAGTGGAAAATGTAGGATAGAATACTTGTACTGTGGCATTGATGCGAATGCCCCCGGATTCAAAGAATGGCAAAAAATCAGAGTGGATCTTCTTAAGAAGAGCTGCACTGCAGAAGGGGAAAATCCGCCATTTAATTTTGGAATCTTCGCAGAGGCTATATCTTCAGATGTCATTGCATCAGCCGAATTTATGTCCAAATTATTGTTCTGCTTGTGGTGGGGACTGAAAAATTTAAGTACACTTGGCCAGGGGCTCGAAACTAGCACGTACCCTCTAGAGGTTCTATTTTCCATTGGGATAGGCATTTTCGGGCTCATCCTCTTTGCCCTTCTGATTGGAAATATCCAGACGTACCTTCAGTCTCTAACAATTCGTCTTGAGGAGATGCGGATTAAAAAGCGTGACTCTGAGCAGTGGATGCACCACCGCTGGCTTCCACAAGAACTTAGGGAAAGGGTTCGGCGCTATGATCAATACAAGTGGTTGGAGACTCGCGGTGTGGATGAGGAGAACATTGTCCAGAGTCTTCCAAAAGATCTAAGAAGGGACATTAAACGCCATCTCTGCTTGAATTTGGTTAGAAGGGTGCCTCTTTTCGCCAATATGGATGAGCGGTTACTTGACGCCATTTGTGAGCGTCTGAAACCAAGTTTATGCACGGATAATACCTACATTGTCCGGGAAGGGGATCCGGTTATTGAGATGCTGTTCATCATACGTGGTCGCCTAGAAAGTGTAACTACAGATGGCGGTAGAAGTGGATTTTTCAACAGCGGTGTACTGAAAGAAGGGGACTTTTGCGGAGAAGAGCTCCTAACATGGGCACTGGATCCTAAAGCTGGTTCTAACTTGCCATCGTCAACTCGAACTGTGAAGGCTTTAACTGAGGTTGAGGCTTTTGCTTTAGAAGCAGAGGAGTTGAAATTTGTTGCCAGTCAGTTTCGGCACCTGCACAGCAGACAGGTTCAGTACACCTTCCGTTTCTACTCCCAGCAGTGGAGGACTTGGGCAGCGTGCTTTATCCAAGCCGCATGGCGCCACTACAGGAGAAAATTGGCTGAAAAACAGCGTAGAGAAGAGGAAGAAGGCTTTGACTACGGTGAAGGAGATGATGATGATGGTGGTGGTGGTGGTGATTTTAAAGGCGGTTCTGGTACAAGCATGCTTCGTTCTGCCATCTATGCATCCCGGTTTGCAGCAAATGCTCTTCGTGGTCATAGGATCCGCAGTGGAAGGGAGCAGATGTTGAAGCTGCAGAAGCCCACTGAACCCGATTTCTCTGCTTATGATGACAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0022414 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032989 GO:0034220 GO:0040007 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0044706 GO:0046873 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051179 GO:0051234 GO:0051704 GO:0055085 GO:0060560 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

743

Amino Acids

84.94

Weight (kDa)

8.88

Isoelectric Point (pI)

50.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	111 - 434	1.7e-18	Ion transport protein
cNMP_binding	PF00027	532 - 619	8.6e-07	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011651)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15990 AT1G19780 AT1G19780
fragaria_vesca	FvH4_6g28560 FvH4_6g28560
malus_domestica	MD05G1041200.v1.1
prunus_persica	Prupe.3G099200_v2.0.a1
pyrus_communis	pycom05g03400
rosa_chinensis	RchiOBHm_Chr2g0132901
rosa_laevigata	RLG00000019289
rosa_multiflora	Rmu_sc0001314.1_g000002 Rmu_sc0016946.1_g000003
rosa_roxburghii	Rroxscaffold_2G00111640
rosa_rugosa	Rorug02G0308100
rosa_samantha	Rh2AG360700 Rh2BG366600 Rh2CG343900 Rh2DG383600
rosa_wichuraiana	Rw2G029330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	779, 1887
Acc36I	ACCTGC	2 cut(s)	779, 1887
AccB1I	GGYRCC	3 cut(s)	1516, 1875, 1965
AccB7I	CCANNNNNTGG	1 cut(s)	652
AccBSI	CCGCTC	2 cut(s)	854, 1543
AccII	CGCG	2 cut(s)	258, 1425
AclWI	GGATC	8 cut(s)	566, 1023, 1613, 1626, 1754, 1767, 2146, 2159
AcoI	YGGCCR	2 cut(s)	37, 1174
AcsI	RAATTY	6 cut(s)	50, 195, 1115, 1159, 1501, 1853
AcuI	CTGAAG	2 cut(s)	1077, 1271
AcyI	GRCGYC	3 cut(s)	249, 1554, 1966
AfeI	AGCGCT	1 cut(s)	896
AfiI	CCNNNNNNNGG	7 cut(s)	149, 652, 1207, 1615, 1750, 1769, 1926
AflII	CTTAAG	1 cut(s)	1022
AgeI	ACCGGT	1 cut(s)	101
AgsI	TTSAA	6 cut(s)	200, 991, 1501, 1699, 1852, 2179
AjnI	CCWGG	1 cut(s)	1176
AjuI	GAANNNNNNNTTGG	2 cut(s)	1118, 1150
AluBI	AGCT	8 cut(s)	521, 761, 887, 920, 1032, 1741, 1769, 2182
AluI	AGCT	8 cut(s)	521, 761, 887, 920, 1032, 1741, 1769, 2182
Alw21I	GWGCWC	3 cut(s)	172, 636, 1743
Alw26I	GTCTC	2 cut(s)	1296, 1412
AlwI	GGATC	8 cut(s)	566, 1023, 1613, 1626, 1754, 1767, 2146, 2159
Ama87I	CYCGRG	1 cut(s)	540
Aor51HI	AGCGCT	1 cut(s)	896
AoxI	GGCC	3 cut(s)	37, 813, 1174
ApeKI	GCWGC	6 cut(s)	821, 869, 1032, 1940, 2126, 2182
ApoI	RAATTY	6 cut(s)	50, 195, 1115, 1159, 1501, 1853
AsiGI	ACCGGT	1 cut(s)	101
Asp700I	GAANNNNTTC	1 cut(s)	1386
AspLEI	GCGC	4 cut(s)	260, 897, 1395, 1968
AspS9I	GGNCC	1 cut(s)	813
AsuC2I	CCSGG	6 cut(s)	269, 817, 865, 984, 1610, 2119
AsuHPI	GGTGA	4 cut(s)	244, 853, 2042, 2075
AvaI	CYCGRG	1 cut(s)	540
BaeGI	GKGCMC	1 cut(s)	1756
BalI	TGGCCA	2 cut(s)	39, 1176
BamHI	GGATCC	3 cut(s)	1618, 1759, 2151
BanI	GGYRCC	3 cut(s)	1516, 1875, 1965
BanII	GRGCYC	3 cut(s)	1185, 1245, 1743
BbsI	GAAGAC	1 cut(s)	1448
Bbv12I	GWGCWC	3 cut(s)	172, 636, 1743
BbvI	GCAGC	6 cut(s)	808, 856, 1019, 1952, 2138, 2169
BccI	CCATC	8 cut(s)	206, 541, 896, 1497, 1670, 1792, 2042, 2114
BcgI	CGANNNNNNTGC	2 cut(s)	2192, 2226
BciT130I	CCWGG	1 cut(s)	1178
BclI	TGATCA	1 cut(s)	1399
BcnI	CCSGG	6 cut(s)	269, 817, 865, 984, 1610, 2119
BcoDI	GTCTC	2 cut(s)	1296, 1412
BfaI	CTAG	3 cut(s)	1191, 1205, 1658
BfmI	CTRYAG	4 cut(s)	1038, 1671, 1972, 2183
BfoI	RGCGCY	3 cut(s)	898, 1396, 1969
BfrI	CTTAAG	1 cut(s)	1022
BfuAI	ACCTGC	2 cut(s)	779, 1887
BglII	AGATCT	2 cut(s)	274, 1465
BisI	GCNGC	8 cut(s)	822, 852, 870, 1033, 1941, 1959, 2127, 2183
BlsI	GCNGC	8 cut(s)	823, 853, 871, 1034, 1942, 1960, 2128, 2184
Bme1390I	CCNGG	7 cut(s)	269, 817, 865, 984, 1178, 1610, 2119
BmeT110I	CYCGRG	1 cut(s)	540
BmgT120I	GGNCC	1 cut(s)	813
BmiI	GGNNCC	7 cut(s)	728, 1518, 1620, 1761, 1877, 1967, 2153
BmrFI	CCNGG	7 cut(s)	269, 817, 865, 984, 1178, 1610, 2119
BmsI	GCATC	9 cut(s)	366, 469, 896, 961, 1115, 1305, 1338, 1623, 2123
BpiI	GAAGAC	1 cut(s)	1448
BplI	GAGNNNNNCTC	2 cut(s)	1725, 1757
BpmI	CTGGAG	1 cut(s)	713
BpuEI	CTTGAG	2 cut(s)	840, 1328
BpuMI	CCSGG	6 cut(s)	269, 817, 865, 984, 1610, 2119
Bsa29I	ATCGAT	1 cut(s)	432
BsaAI	YACGTR	3 cut(s)	81, 1197, 1649
BsaHI	GRCGYC	3 cut(s)	249, 1554, 1966
BsaJI	CCNNGG	4 cut(s)	816, 864, 982, 1177
BsaWI	WCCGGW	2 cut(s)	101, 1621
BsaXI	ACNNNNNCTCC	4 cut(s)	775, 805, 2159, 2189
Bsc4I	CCNNNNNNNGG	7 cut(s)	149, 652, 1207, 1615, 1750, 1769, 1926
Bse118I	RCCGGY	1 cut(s)	101
Bse1I	ACTGG	5 cut(s)	154, 250, 730, 1761, 1863
Bse3DI	GCAATG	2 cut(s)	707, 1101
BseBI	CCWGG	1 cut(s)	1178
BseCI	ATCGAT	1 cut(s)	432
BseDI	CCNNGG	4 cut(s)	816, 864, 982, 1177
BseGI	GGATG	7 cut(s)	70, 911, 1245, 1353, 1438, 1543, 2114
BseLI	CCNNNNNNNGG	7 cut(s)	149, 652, 1207, 1615, 1750, 1769, 1926
BseMI	GCAATG	2 cut(s)	707, 1101
BseMII	CTCAG	3 cut(s)	783, 1329, 1806
BseNI	ACTGG	5 cut(s)	154, 250, 730, 1761, 1863
BseRI	GAGGAG	3 cut(s)	1325, 1451, 1859
BseSI	GKGCMC	1 cut(s)	1756
BseXI	GCAGC	6 cut(s)	808, 856, 1019, 1952, 2138, 2169
BseYI	CCCAGC	1 cut(s)	1920
BsgI	GTGCAG	3 cut(s)	807, 1018, 1865
Bsh1236I	CGCG	2 cut(s)	258, 1425
Bsh1285I	CGRYCG	1 cut(s)	436
BshFI	GGCC	3 cut(s)	39, 815, 1176
BshNI	GGYRCC	3 cut(s)	1516, 1875, 1965
BshTI	ACCGGT	1 cut(s)	101
BshVI	ATCGAT	1 cut(s)	432
BsiEI	CGRYCG	1 cut(s)	436
BsiHKAI	GWGCWC	3 cut(s)	172, 636, 1743
BsiHKCI	CYCGRG	1 cut(s)	540
BsiSI	CCGG	8 cut(s)	102, 269, 816, 864, 984, 1609, 1622, 2119
BslFI	GGGAC	3 cut(s)	1164, 1490, 1736
BslI	CCNNNNNNNGG	7 cut(s)	149, 652, 1207, 1615, 1750, 1769, 1926
BsmAI	GTCTC	2 cut(s)	1296, 1412
BsmFI	GGGAC	3 cut(s)	1164, 1490, 1736
BsmI	GAATGC	1 cut(s)	982
BsnI	GGCC	3 cut(s)	39, 815, 1176
BsoBI	CYCGRG	1 cut(s)	540
Bsp1286I	GDGCHC	6 cut(s)	172, 636, 1185, 1245, 1743, 1756
BspANI	GGCC	3 cut(s)	39, 815, 1176
BspCNI	CTCAG	3 cut(s)	782, 1330, 1807
BspDI	ATCGAT	1 cut(s)	432
BspFNI	CGCG	2 cut(s)	258, 1425
BspLI	GGNNCC	7 cut(s)	728, 1518, 1620, 1761, 1877, 1967, 2153
BspMAI	CTGCAG	2 cut(s)	1042, 2187
BspMI	ACCTGC	2 cut(s)	779, 1887
BspPI	GGATC	8 cut(s)	566, 1023, 1613, 1626, 1754, 1767, 2146, 2159
BspQI	GCTCTTC	3 cut(s)	1022, 1731, 2142
BspT107I	GGYRCC	3 cut(s)	1516, 1875, 1965
BspTI	CTTAAG	1 cut(s)	1022
BsrBI	CCGCTC	2 cut(s)	854, 1543
BsrDI	GCAATG	2 cut(s)	707, 1101
BsrFI	RCCGGY	1 cut(s)	101
BsrI	ACTGG	5 cut(s)	154, 250, 730, 1761, 1863
BssAI	RCCGGY	1 cut(s)	101
BssECI	CCNNGG	4 cut(s)	816, 864, 982, 1177
BssNI	GRCGYC	3 cut(s)	249, 1554, 1966
Bst2UI	CCWGG	1 cut(s)	1178
Bst4CI	ACNGT	9 cut(s)	27, 60, 174, 468, 510, 605, 962, 1801, 2030
Bst6I	CTCTTC	6 cut(s)	547, 670, 1022, 1731, 2001, 2142
BstACI	GRCGYC	3 cut(s)	249, 1554, 1966
BstAFI	CTTAAG	1 cut(s)	1022
BstAPI	GCANNNNNTGC	2 cut(s)	1348, 2132
BstBAI	YACGTR	3 cut(s)	81, 1197, 1649
BstC8I	GCNNGC	5 cut(s)	849, 911, 1361, 1945, 2093
BstDEI	CTNAG	6 cut(s)	613, 769, 1338, 1376, 1469, 1815
BstF5I	GGATG	7 cut(s)	70, 911, 1245, 1353, 1438, 1543, 2114
BstFNI	CGCG	2 cut(s)	258, 1425
BstH2I	RGCGCY	3 cut(s)	898, 1396, 1969
BstHHI	GCGC	4 cut(s)	260, 897, 1395, 1968
BstMAI	GTCTC	2 cut(s)	1296, 1412
BstMCI	CGRYCG	1 cut(s)	436
BstNI	CCWGG	1 cut(s)	1178
BstNSI	RCATGY	1 cut(s)	2095
BstSCI	CCNGG	7 cut(s)	267, 815, 863, 982, 1176, 1608, 2117
BstSFI	CTRYAG	4 cut(s)	1038, 1671, 1972, 2183
BstSLI	GKGCMC	1 cut(s)	1756
BstSNI	TACGTA	1 cut(s)	81
BstUI	CGCG	2 cut(s)	258, 1425
BstV1I	GCAGC	6 cut(s)	808, 856, 1019, 1952, 2138, 2169
BstV2I	GAAGAC	1 cut(s)	1448
BstX2I	RGATCY	7 cut(s)	274, 571, 1015, 1465, 1618, 1759, 2151
BstYI	RGATCY	7 cut(s)	274, 571, 1015, 1465, 1618, 1759, 2151
Bsu15I	ATCGAT	1 cut(s)	432
BsuRI	GGCC	3 cut(s)	39, 815, 1176
BsuTUI	ATCGAT	1 cut(s)	432
BtsCI	GGATG	7 cut(s)	70, 911, 1245, 1353, 1438, 1543, 2114
BtsI	GCAGTG	5 cut(s)	267, 1035, 1349, 1931, 2164
BtsIMutI	CAGTG	7 cut(s)	267, 1035, 1349, 1754, 1931, 2164, 2193
BveI	ACCTGC	2 cut(s)	779, 1887
Cac8I	GCNNGC	5 cut(s)	849, 911, 1361, 1945, 2093
CfoI	GCGC	4 cut(s)	260, 897, 1395, 1968
Cfr10I	RCCGGY	1 cut(s)	101
Cfr13I	GGNCC	1 cut(s)	813
ClaI	ATCGAT	1 cut(s)	432
CseI	GACGC	3 cut(s)	238, 1556, 1562
CspAI	ACCGGT	1 cut(s)	101
CspCI	CAANNNNNGTGG	2 cut(s)	1391, 1426
CviAII	CATG	7 cut(s)	88, 499, 671, 873, 1749, 1962, 2092
DdeI	CTNAG	6 cut(s)	613, 769, 1338, 1376, 1469, 1815
DinI	GGCGCC	1 cut(s)	1967
DraI	TTTAAA	2 cut(s)	765, 2071
EaeI	YGGCCR	2 cut(s)	37, 1174
Eam1104I	CTCTTC	6 cut(s)	547, 670, 1022, 1731, 2001, 2142
EarI	CTCTTC	6 cut(s)	547, 670, 1022, 1731, 2001, 2142
EciI	GGCGGA	1 cut(s)	1044
Ecl136II	GAGCTC	1 cut(s)	1741
Eco105I	TACGTA	1 cut(s)	81
Eco24I	GRGCYC	3 cut(s)	1185, 1245, 1743
Eco32I	GATATC	1 cut(s)	596
Eco47III	AGCGCT	1 cut(s)	896
Eco53kI	GAGCTC	1 cut(s)	1741
Eco57I	CTGAAG	2 cut(s)	1077, 1271
Eco88I	CYCGRG	1 cut(s)	540
EcoICRI	GAGCTC	1 cut(s)	1741
EcoRI	GAATTC	1 cut(s)	195
EcoRII	CCWGG	1 cut(s)	1176
EcoRV	GATATC	1 cut(s)	596
EcoT22I	ATGCAT	1 cut(s)	2116
EcoT38I	GRGCYC	3 cut(s)	1185, 1245, 1743
EgeI	GGCGCC	1 cut(s)	1967
EheI	GGCGCC	1 cut(s)	1967
FaeI	CATG	7 cut(s)	91, 502, 674, 876, 1752, 1965, 2095
FaqI	GGGAC	3 cut(s)	1164, 1490, 1736
FatI	CATG	7 cut(s)	87, 498, 670, 872, 1748, 1961, 2091
FauI	CCCGC	2 cut(s)	263, 906
FbaI	TGATCA	1 cut(s)	1399
Fnu4HI	GCNGC	8 cut(s)	822, 852, 870, 1033, 1941, 1959, 2127, 2183
FokI	GGATG	7 cut(s)	77, 918, 1232, 1360, 1445, 1550, 2101
FriOI	GRGCYC	3 cut(s)	1185, 1245, 1743
Fsp4HI	GCNGC	8 cut(s)	822, 852, 870, 1033, 1941, 1959, 2127, 2183
FspBI	CTAG	3 cut(s)	1191, 1205, 1658
GlaI	GCGC	4 cut(s)	259, 896, 1394, 1967
GluI	GCNGC	8 cut(s)	822, 852, 870, 1033, 1941, 1959, 2127, 2183
GsaI	CCCAGC	1 cut(s)	1924
GsuI	CTGGAG	1 cut(s)	713
HaeII	RGCGCY	3 cut(s)	898, 1396, 1969
HaeIII	GGCC	3 cut(s)	39, 815, 1176
HapII	CCGG	8 cut(s)	102, 269, 816, 864, 984, 1609, 1622, 2119
HgaI	GACGC	3 cut(s)	238, 1556, 1562
HhaI	GCGC	4 cut(s)	260, 897, 1395, 1968
Hin1I	GRCGYC	3 cut(s)	249, 1554, 1966
Hin1II	CATG	7 cut(s)	91, 502, 674, 876, 1752, 1965, 2095
Hin6I	GCGC	4 cut(s)	258, 895, 1393, 1966
HinP1I	GCGC	4 cut(s)	258, 895, 1393, 1966
HincII	GTYRAC	2 cut(s)	619, 1791
HindII	GTYRAC	2 cut(s)	619, 1791
HinfI	GANTC	8 cut(s)	134, 663, 680, 987, 1071, 1334, 1420, 1453
HpaII	CCGG	8 cut(s)	102, 269, 816, 864, 984, 1609, 1622, 2119
HphI	GGTGA	4 cut(s)	244, 853, 2042, 2075
Hpy166II	GTNNAC	5 cut(s)	619, 1169, 1709, 1791, 1902
Hpy188III	TCNNGA	4 cut(s)	1205, 1276, 1307, 1450
Hpy8I	GTNNAC	5 cut(s)	619, 1169, 1709, 1791, 1902
HpyCH4III	ACNGT	9 cut(s)	27, 60, 174, 468, 510, 605, 962, 1801, 2030
HpyCH4IV	ACGT	4 cut(s)	80, 1196, 1280, 1648
HpyF3I	CTNAG	6 cut(s)	613, 769, 1338, 1376, 1469, 1815
HpySE526I	ACGT	4 cut(s)	80, 1196, 1280, 1648
Hsp92I	GRCGYC	3 cut(s)	249, 1554, 1966
Hsp92II	CATG	7 cut(s)	91, 502, 674, 876, 1752, 1965, 2095
HspAI	GCGC	4 cut(s)	258, 895, 1393, 1966
KasI	GGCGCC	1 cut(s)	1965
Ksp22I	TGATCA	1 cut(s)	1399
LguI	GCTCTTC	3 cut(s)	1022, 1731, 2142
LmnI	GCTCC	7 cut(s)	535, 639, 732, 892, 917, 1746, 2167
Lsp1109I	GCAGC	6 cut(s)	808, 856, 1019, 1952, 2138, 2169
LweI	GCATC	9 cut(s)	366, 469, 896, 961, 1115, 1305, 1338, 1623, 2123
MaeI	CTAG	3 cut(s)	1191, 1205, 1658
MaeII	ACGT	4 cut(s)	80, 1196, 1280, 1648
MaeIII	GTNAC	7 cut(s)	174, 250, 732, 859, 1331, 1545, 1666
MbiI	CCGCTC	2 cut(s)	854, 1543
MflI	RGATCY	7 cut(s)	274, 571, 1015, 1465, 1618, 1759, 2151
MhlI	GDGCHC	6 cut(s)	172, 636, 1185, 1245, 1743, 1756
MlsI	TGGCCA	2 cut(s)	39, 1176
MluNI	TGGCCA	2 cut(s)	39, 1176
Mly113I	GGCGCC	1 cut(s)	1966
MlyI	GAGTC	5 cut(s)	143, 689, 1328, 1414, 1462
MmeI	TCCRAC	2 cut(s)	42, 1395
Mox20I	TGGCCA	2 cut(s)	39, 1176
Mph1103I	ATGCAT	1 cut(s)	2116
MroXI	GAANNNNTTC	1 cut(s)	1386
MscI	TGGCCA	2 cut(s)	39, 1176
Msp20I	TGGCCA	2 cut(s)	39, 1176
MspA1I	CMGCKG	2 cut(s)	1359, 1704
MspCI	CTTAAG	1 cut(s)	1022
MspI	CCGG	8 cut(s)	102, 269, 816, 864, 984, 1609, 1622, 2119
MspR9I	CCNGG	7 cut(s)	269, 817, 865, 984, 1178, 1610, 2119
Mva1269I	GAATGC	1 cut(s)	982
MvaI	CCWGG	1 cut(s)	1178
MvnI	CGCG	2 cut(s)	258, 1425
NarI	GGCGCC	1 cut(s)	1966
NciI	CCSGG	6 cut(s)	269, 817, 865, 984, 1610, 2119
NlaIII	CATG	7 cut(s)	91, 502, 674, 876, 1752, 1965, 2095
NlaIV	GGNNCC	7 cut(s)	728, 1518, 1620, 1761, 1877, 1967, 2153
NmuCI	GTSAC	4 cut(s)	174, 250, 859, 1331
NsiI	ATGCAT	1 cut(s)	2116
NspI	RCATGY	1 cut(s)	2095
PaeI	GCATGC	1 cut(s)	2095
PaqCI	CACCTGC	2 cut(s)	779, 1887
PciSI	GCTCTTC	3 cut(s)	1022, 1731, 2142
PcsI	WCGNNNNNNNCGW	1 cut(s)	555
PctI	GAATGC	1 cut(s)	982
PdmI	GAANNNNTTC	1 cut(s)	1386
PfeI	GAWTC	3 cut(s)	663, 987, 1071
PflMI	CCANNNNNTGG	1 cut(s)	652
PfoI	TCCNGGA	2 cut(s)	267, 1608
PinAI	ACCGGT	1 cut(s)	101
PkrI	GCNGC	8 cut(s)	823, 853, 871, 1034, 1942, 1960, 2128, 2184
Ple19I	CGATCG	1 cut(s)	436
PleI	GAGTC	5 cut(s)	142, 688, 1328, 1414, 1461
PluTI	GGCGCC	1 cut(s)	1969
PpsI	GAGTC	5 cut(s)	142, 688, 1328, 1414, 1461
Ppu21I	YACGTR	3 cut(s)	81, 1197, 1649
Psp124BI	GAGCTC	1 cut(s)	1743
Psp6I	CCWGG	1 cut(s)	1176
PspFI	CCCAGC	1 cut(s)	1920
PspGI	CCWGG	1 cut(s)	1176
PspN4I	GGNNCC	7 cut(s)	728, 1518, 1620, 1761, 1877, 1967, 2153
PspPI	GGNCC	1 cut(s)	813
PstI	CTGCAG	2 cut(s)	1042, 2187
PsuI	RGATCY	7 cut(s)	274, 571, 1015, 1465, 1618, 1759, 2151
PvuI	CGATCG	1 cut(s)	436
SacI	GAGCTC	1 cut(s)	1743
SapI	GCTCTTC	3 cut(s)	1022, 1731, 2142
SatI	GCNGC	8 cut(s)	822, 852, 870, 1033, 1941, 1959, 2127, 2183
Sau96I	GGNCC	1 cut(s)	813
SchI	GAGTC	5 cut(s)	143, 689, 1328, 1414, 1462
ScrFI	CCNGG	7 cut(s)	269, 817, 865, 984, 1178, 1610, 2119
SduI	GDGCHC	6 cut(s)	172, 636, 1185, 1245, 1743, 1756
SfaNI	GCATC	9 cut(s)	366, 469, 896, 961, 1115, 1305, 1338, 1623, 2123
SfcI	CTRYAG	4 cut(s)	1038, 1671, 1972, 2183
SfoI	GGCGCC	1 cut(s)	1967
SmlI	CTYRAG	3 cut(s)	855, 1022, 1307
SmoI	CTYRAG	3 cut(s)	855, 1022, 1307
SnaBI	TACGTA	1 cut(s)	81
SphI	GCATGC	1 cut(s)	2095
SspDI	GGCGCC	1 cut(s)	1965
SspMI	CTAG	3 cut(s)	1191, 1205, 1658
SstI	GAGCTC	1 cut(s)	1743
StyD4I	CCNGG	7 cut(s)	267, 815, 863, 982, 1176, 1608, 2117
TaaI	ACNGT	9 cut(s)	27, 60, 174, 468, 510, 605, 962, 1801, 2030
TaiI	ACGT	4 cut(s)	83, 1199, 1283, 1651
TaqI	TCGA	7 cut(s)	6, 132, 405, 432, 436, 1185, 1795
TatI	WGTACW	4 cut(s)	957, 1166, 1708, 1899
TauI	GCSGC	2 cut(s)	854, 1961
TfiI	GAWTC	3 cut(s)	663, 987, 1071
TscAI	CASTG	7 cut(s)	267, 1042, 1349, 1761, 1931, 2164, 2200
TseFI	GTSAC	4 cut(s)	174, 250, 859, 1331
TseI	GCWGC	6 cut(s)	821, 869, 1032, 1940, 2126, 2182
Tsp45I	GTSAC	4 cut(s)	174, 250, 859, 1331
TspDTI	ATGAA	2 cut(s)	125, 1630
TspGWI	ACGGA	2 cut(s)	1604, 1898
TspRI	CASTG	7 cut(s)	267, 1042, 1349, 1761, 1931, 2164, 2200
Van91I	CCANNNNNTGG	1 cut(s)	652
Vha464I	CTTAAG	1 cut(s)	1022
XapI	RAATTY	6 cut(s)	50, 195, 1115, 1159, 1501, 1853
XbaI	TCTAGA	1 cut(s)	1204
XceI	RCATGY	1 cut(s)	2095
XmnI	GAANNNNTTC	1 cut(s)	1386
XspI	CTAG	3 cut(s)	1191, 1205, 1658
Zsp2I	ATGCAT	1 cut(s)	2116

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.