MD05G1055900.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014653
Physical Location & Seq
Reverse (-)
9829468 .. 9835319
5852 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1055900.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGGCTTCTCCTTGTGGGGTTTTCGGCACCCTTTTTCTTCTTCTTGCTCTACAGAATTCTGGGTTTCCTCTCTCTTTGGCTTCCAAAAATGTTTACATTGTATATATGGGAGAAAGACAGCATGGAGAGCCTGAGCTGGTTTCTGAGTCTCACCATCAGACTCTGTCATCTGTATTTGGAAGCAAAGAAGCCGCAAAGGAATCAATTCTGTACAACTACAAGCATGGATTTTCAGGGTTCGCTGCTGTCCTAACTCCATGTCAAGCAAAGCTTATTGCAGATTCCCCTGGCGTTGTTCATGTCATTCCAAACAGAATTCTTAATTTGCATACGACTAGAAGTTGGAATTTTCTGGAAGTAAACTCTCATATTAGAAATGGAATTCTTTCAAAGAGTCAATCAGGCATTGGGTCTATTATTGGAATCATGGACACTGGAATTTGGCCTGAGTCTGAAAGCTTCAGAGATGAGGGTATGGGAGACGCCCCATCCCGATTCAGGGGGATATGTCAAGAAGGAGAAAAGTTCAATCGCTCCCATTGTAACCGGAAGATAATTGGTGCACGGTGGTATAGTAAAGGATATGAAGCTGAATTCGGAAATCTAAGTACAAGTGATGAGTTTGAATATCTATCTCCCCGAGATGCTGCAGGACATGGTACTCATACATCATCTACCGCAGCTGGTGATTCAATAGAAAATGCAAGCTATAAGGGATTATCTGCGGGATTGGCAAGAGGGGGTGCTCCATCAGCTCGGTTAGCTGTCTATAAAGTTTGTTGGGCAACTGGTGGCTGCAGCTCAGCAGACCTGCTTGCCGCATTTGATGATGCAATCTATGATGGTGTAGATGTGTTGTCGGTATCTCTAGGTTCTCCACCTCCACTTTCTTCTTATGTTGAGGATGCTGTGGCCATTGGCTCTTTCCATGCTGTTGCCAGAGGAATCTCTGTTATATGCTCTGCTGGAAATTCTGGTCCATACCCTCAAACTGTCATAAATTCTGCTCCCTGGATTTTCACTGTTGCGGCAAGCACTATTGATAGGGCTTTCCCTACTGTGATTACCCTGGGAAACAATCAAACTGTTGTGGGTCAGGCTTTGTATACAGGGATGGATATAAACAAGTATTACCCTCTTGTTTATGGAGGAGACATTGCATCTATTGATTCAGATGAAGACACTGCTGGGAGCTGTGATCCAGGAACTCTAAATGAAACTTTAGCAACTGGAAAGGTCATTCTTTGTTTCCAATCTCAATCACAGAGGTTCTCTATTCCTGCAACGACAACTGTGACGAGTGCTAGGGGCGCTGGTCTCATTTTTGCTCAGTTTCCTAGCAAGGATGTTTCTTTGTCTTCGGGTAGTCTACCTTGTGTCCAAGTTGACTTTGCAATTGGCACAAATCTGCTAACATACATTGGAGCAACCAGAAATCCCGTAGTCAAGTTCAATCCTTCAAAAACACTAGTGGGACAACAGATATCCCCAGAAGTGGCATTTTTCTCTTCCCGAGGACCCAATTCTCTCTCTCCTTCCGTACTTAAGCCAGACATAGCTGCTCCGGGTGTTAATATATTAGCCTCCTGGTCTCCTGCTCGGTCCCAAAATCAAGCAAATCCGGATATTTTCAAAATTGAATCAGGAACTTCCATGGCTTGTCCCCATATATCCGGTATTGTTGCTCTTCTTAAATCTATCCATCCAACGTGGAGTCCTGCTGCAATAAAGTCTGCTCTGGTTACAACAGCTTCTCTAGAAGATGAATATGGTCAAAGTATTGTGGCCGAGGGGGCTCCTCACAAGCAGGCCGATCCATTTGACTATGGTGGTGGTCATGTTGATCCTAACAAAGCCATAGCTCCTGGTCTCATATATGACATAGAATCATCAGATTATATCCGTTTCCTGTGCTCCATGGGTTATAACAACACAGCCATCAGTTTAGTGGCTGGGGCTAAGATCACATGCCGTAAATCAACCAACTTCATTAATAACCTTAATCTTCCTTCTATCACCATTCATGAGCTGAAGCAGAGAACAACAGTATCAAGAACGGTCACAAATGTAGGTCCAGTTAATTCTATCTACAATGCTCGTGTTATAACGCCTGCAGGTGTGTCTGTGAGAGTTAAGCCATCAATTTTGTCATTCAACTCTACTGTAAAGAAGCTGGAGTTTAAGGTTACTTTTCGTTCCCTATTAAGAATTCAAGGAAGATTCTCCTTCGGAAATCTGTTTTGGGAAGATGGTTTGCATGTAGTTAGGATACCACTCATAGTTCGAATTGTCCTTGATAATTTTTATGCAGACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

773

Amino Acids

82.42

Weight (kDa)

6.79

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 110 1.2e-20 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 588 1e-44 Subtilase family
fn3_6 PF17766 666 - 761 8e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014653)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10210 FvH4_2g10210 FvH4_2g10210
malus_domestica MD05G1055900.v1.1
prunus_persica Prupe.8G086300_v2.0.a1
pyrus_communis pycom05g04760
rosa_chinensis RchiOBHm_Chr6g0267441
rosa_laevigata RLG00000014004
rosa_multiflora Rmu_sc0001068.1_g000015 Rmu_sc0006674.1_g000011
rosa_roxburghii Rroxscaffold_7G00200780
rosa_rugosa Rorug06G0032600 Rorug06G0032600
rosa_samantha Rh6AG154000 Rh6BG154100 Rh6CG149800
rosa_wichuraiana Rw6G013220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1926, 2105
AarI CACCTGC 1 cut(s) 2105
Acc36I ACCTGC 2 cut(s) 819, 2105
AccB1I GGYRCC 1 cut(s) 26
AccI GTMKAC 2 cut(s) 1106, 1369
AccIII TCCGGA 1 cut(s) 1621
AciI CCGC 5 cut(s) 192, 678, 725, 819, 1028
AclWI GGATC 3 cut(s) 1193, 1808, 1838
AcoI YGGCCR 2 cut(s) 912, 1785
AcsI RAATTY 9 cut(s) 55, 315, 346, 381, 438, 593, 970, 1000, 2208
AcuI CTGAAG 2 cut(s) 445, 2051
AcyI GRCGYC 1 cut(s) 483
AfaI GTAC 4 cut(s) 212, 610, 661, 1542
AfiI CCNNNNNNNGG 3 cut(s) 498, 499, 1495
AflII CTTAAG 1 cut(s) 1544
AhlI ACTAGT 1 cut(s) 1468
AjnI CCWGG 6 cut(s) 286, 1010, 1068, 1201, 1586, 1864
AjuI GAANNNNNNNTTGG 2 cut(s) 2224, 2256
Alw21I GWGCWC 3 cut(s) 565, 748, 1916
Alw26I GTCTC 6 cut(s) 153, 474, 1146, 1322, 1596, 1874
Alw44I GTGCAC 1 cut(s) 561
AlwI GGATC 3 cut(s) 1193, 1808, 1838
AlwNI CAGNNNCTG 1 cut(s) 163
Ama87I CYCGRG 2 cut(s) 639, 1512
Aor13HI TCCGGA 1 cut(s) 1621
AoxI GGCC 4 cut(s) 443, 912, 1785, 1809
ApaLI GTGCAC 1 cut(s) 561
ApeKI GCWGC 7 cut(s) 242, 647, 680, 795, 798, 1559, 1721
ApoI RAATTY 9 cut(s) 55, 315, 346, 381, 438, 593, 970, 1000, 2208
AseI ATTAAT 1 cut(s) 1992
Asp700I GAANNNNTTC 2 cut(s) 204, 385
AspLEI GCGC 1 cut(s) 1313
AspS9I GGNCC 4 cut(s) 977, 1517, 1602, 2072
AsuC2I CCSGG 1 cut(s) 1566
AsuHPI GGTGA 3 cut(s) 143, 698, 2008
AsuII TTCGAA 1 cut(s) 2284
AvaI CYCGRG 2 cut(s) 639, 1512
AvaII GGWCC 4 cut(s) 977, 1517, 1602, 2072
BaeGI GKGCMC 1 cut(s) 565
BaeI ACNNNNGTAYC 2 cut(s) 2031, 2064
BalI TGGCCA 1 cut(s) 914
BanI GGYRCC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 1798
BauI CACGAG 1 cut(s) 2097
BbsI GAAGAC 2 cut(s) 1185, 1350
Bbv12I GWGCWC 3 cut(s) 565, 748, 1916
BbvI GCAGC 7 cut(s) 229, 634, 692, 782, 810, 1546, 1708
BccI CCATC 9 cut(s) 162, 496, 757, 836, 1108, 1710, 1946, 2146, 2243
BceAI ACGGC 1 cut(s) 1956
BciT130I CCWGG 6 cut(s) 288, 1012, 1070, 1203, 1588, 1866
BciVI GTATCC 1 cut(s) 2262
BcnI CCSGG 1 cut(s) 1566
BcoDI GTCTC 6 cut(s) 153, 474, 1146, 1322, 1596, 1874
BcuI ACTAGT 1 cut(s) 1468
BfaI CTAG 6 cut(s) 336, 869, 1305, 1338, 1469, 1757
BfmI CTRYAG 4 cut(s) 50, 648, 796, 2112
BfoI RGCGCY 1 cut(s) 1314
BfrI CTTAAG 1 cut(s) 1544
BfuAI ACCTGC 2 cut(s) 819, 2105
BfuI GTATCC 1 cut(s) 2262
BglI GCCNNNNNGGC 1 cut(s) 1793
BlpI GCTNAGC 1 cut(s) 802
Bme1390I CCNGG 7 cut(s) 288, 1012, 1070, 1203, 1566, 1588, 1866
Bme18I GGWCC 4 cut(s) 977, 1517, 1602, 2072
BmeT110I CYCGRG 2 cut(s) 639, 1512
BmgT120I GGNCC 4 cut(s) 977, 1517, 1602, 2072
BmiI GGNNCC 4 cut(s) 28, 1519, 1604, 1797
BmrFI CCNGG 7 cut(s) 288, 1012, 1070, 1203, 1566, 1588, 1866
BmsI GCATC 4 cut(s) 634, 820, 895, 1169
BpiI GAAGAC 2 cut(s) 1185, 1350
BpmI CTGGAG 1 cut(s) 2195
Bpu10I CCTNAGC 1 cut(s) 132
Bpu1102I GCTNAGC 1 cut(s) 802
Bpu14I TTCGAA 1 cut(s) 2284
BpuMI CCSGG 1 cut(s) 1566
BsaHI GRCGYC 1 cut(s) 483
BsaI GGTCTC 3 cut(s) 1322, 1596, 1874
BsaJI CCNNGG 8 cut(s) 286, 1010, 1068, 1069, 1513, 1653, 1788, 1917
BsaWI WCCGGW 3 cut(s) 546, 1621, 1673
Bsc4I CCNNNNNNNGG 3 cut(s) 498, 499, 1495
Bse1I ACTGG 4 cut(s) 439, 793, 1234, 2075
Bse3DI GCAATG 1 cut(s) 1155
BseAI TCCGGA 1 cut(s) 1621
BseBI CCWGG 6 cut(s) 288, 1012, 1070, 1203, 1588, 1866
BseDI CCNNGG 8 cut(s) 286, 1010, 1068, 1069, 1513, 1653, 1788, 1917
BseGI GGATG 5 cut(s) 488, 910, 1119, 1351, 1702
BseLI CCNNNNNNNGG 3 cut(s) 498, 499, 1495
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 5 cut(s) 123, 135, 438, 816, 1343
BseNI ACTGG 4 cut(s) 439, 793, 1234, 2075
BseRI GAGGAG 2 cut(s) 1164, 1788
BseSI GKGCMC 1 cut(s) 565
BseXI GCAGC 7 cut(s) 229, 634, 692, 782, 810, 1546, 1708
BseYI CCCAGC 2 cut(s) 1187, 1952
BshFI GGCC 4 cut(s) 445, 914, 1787, 1811
BshNI GGYRCC 1 cut(s) 26
BsiHKAI GWGCWC 3 cut(s) 565, 748, 1916
BsiHKCI CYCGRG 2 cut(s) 639, 1512
BsiSI CCGG 4 cut(s) 547, 1565, 1622, 1674
BslFI GGGAC 3 cut(s) 1488, 1588, 1647
BslI CCNNNNNNNGG 3 cut(s) 498, 499, 1495
BsmAI GTCTC 6 cut(s) 153, 474, 1146, 1322, 1596, 1874
BsmBI CGTCTC 1 cut(s) 474
BsmFI GGGAC 3 cut(s) 1488, 1588, 1647
BsnI GGCC 4 cut(s) 445, 914, 1787, 1811
Bso31I GGTCTC 3 cut(s) 1322, 1596, 1874
BsoBI CYCGRG 2 cut(s) 639, 1512
Bsp119I TTCGAA 1 cut(s) 2284
Bsp1286I GDGCHC 4 cut(s) 565, 748, 1798, 1916
Bsp13I TCCGGA 1 cut(s) 1621
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 4 cut(s) 1198, 1813, 1843, 1962
Bsp1720I GCTNAGC 1 cut(s) 802
Bsp19I CCATGG 2 cut(s) 1653, 1917
BspACI CCGC 5 cut(s) 192, 678, 725, 819, 1028
BspANI GGCC 4 cut(s) 445, 914, 1787, 1811
BspCNI CTCAG 5 cut(s) 124, 136, 439, 815, 1342
BspEI TCCGGA 1 cut(s) 1621
BspHI TCATGA 1 cut(s) 2023
BspLI GGNNCC 4 cut(s) 28, 1519, 1604, 1797
BspMAI CTGCAG 3 cut(s) 652, 800, 2116
BspMI ACCTGC 2 cut(s) 819, 2105
BspPI GGATC 3 cut(s) 1193, 1808, 1838
BspQI GCTCTTC 1 cut(s) 1692
BspT104I TTCGAA 1 cut(s) 2284
BspT107I GGYRCC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 1544
BspTNI GGTCTC 3 cut(s) 1322, 1596, 1874
BsrDI GCAATG 1 cut(s) 1155
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 4 cut(s) 439, 793, 1234, 2075
BssECI CCNNGG 8 cut(s) 286, 1010, 1068, 1069, 1513, 1653, 1788, 1917
BssMI GATC 4 cut(s) 1198, 1813, 1843, 1962
BssNAI GTATAC 1 cut(s) 1107
BssNI GRCGYC 1 cut(s) 483
BssSI CACGAG 1 cut(s) 2097
BssT1I CCWWGG 2 cut(s) 1653, 1917
Bst1107I GTATAC 1 cut(s) 1107
Bst2BI CACGAG 1 cut(s) 2097
Bst2UI CCWGG 6 cut(s) 288, 1012, 1070, 1203, 1588, 1866
Bst4CI ACNGT 9 cut(s) 567, 994, 1024, 1060, 1087, 1294, 2047, 2059, 2164
Bst6I CTCTTC 2 cut(s) 1513, 1692
BstACI GRCGYC 1 cut(s) 483
BstAFI CTTAAG 1 cut(s) 1544
BstAUI TGTACA 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 2284
BstC8I GCNNGC 5 cut(s) 706, 816, 1033, 1809, 2112
BstDEI CTNAG 7 cut(s) 132, 144, 447, 605, 802, 1329, 1959
BstDSI CCRYGG 2 cut(s) 1653, 1917
BstF5I GGATG 5 cut(s) 488, 910, 1119, 1351, 1702
BstH2I RGCGCY 1 cut(s) 1314
BstHHI GCGC 1 cut(s) 1313
BstKTI GATC 4 cut(s) 1201, 1816, 1846, 1965
BstMAI GTCTC 6 cut(s) 153, 474, 1146, 1322, 1596, 1874
BstMBI GATC 4 cut(s) 1198, 1813, 1843, 1962
BstMWI GCNNNNNNNGC 6 cut(s) 127, 731, 752, 761, 1310, 1793
BstNI CCWGG 6 cut(s) 288, 1012, 1070, 1203, 1588, 1866
BstNSI RCATGY 2 cut(s) 1971, 2261
BstSCI CCNGG 7 cut(s) 286, 1010, 1068, 1201, 1564, 1586, 1864
BstSFI CTRYAG 4 cut(s) 50, 648, 796, 2112
BstSLI GKGCMC 1 cut(s) 565
BstV1I GCAGC 7 cut(s) 229, 634, 692, 782, 810, 1546, 1708
BstV2I GAAGAC 2 cut(s) 1185, 1350
BstZ17I GTATAC 1 cut(s) 1107
BsuI GTATCC 1 cut(s) 2262
BsuRI GGCC 4 cut(s) 445, 914, 1787, 1811
BtgI CCRYGG 2 cut(s) 1653, 1917
BtsCI GGATG 5 cut(s) 488, 910, 1119, 1351, 1702
BtsI GCAGTG 1 cut(s) 1182
BtsIMutI CAGTG 3 cut(s) 432, 1020, 1182
BveI ACCTGC 2 cut(s) 819, 2105
Cac8I GCNNGC 5 cut(s) 706, 816, 1033, 1809, 2112
CaiI CAGNNNCTG 1 cut(s) 163
CciI TCATGA 1 cut(s) 2023
CfoI GCGC 1 cut(s) 1313
Cfr13I GGNCC 4 cut(s) 977, 1517, 1602, 2072
CseI GACGC 1 cut(s) 491
Csp6I GTAC 4 cut(s) 211, 609, 660, 1541
CviQI GTAC 4 cut(s) 211, 609, 660, 1541
DdeI CTNAG 7 cut(s) 132, 144, 447, 605, 802, 1329, 1959
DpnI GATC 4 cut(s) 1200, 1815, 1845, 1964
DpnII GATC 4 cut(s) 1198, 1813, 1843, 1962
EaeI YGGCCR 2 cut(s) 912, 1785
Eam1104I CTCTTC 2 cut(s) 1513, 1692
EarI CTCTTC 2 cut(s) 1513, 1692
Eco130I CCWWGG 2 cut(s) 1653, 1917
Eco24I GRGCYC 1 cut(s) 1798
Eco31I GGTCTC 3 cut(s) 1322, 1596, 1874
Eco32I GATATC 1 cut(s) 1485
Eco47I GGWCC 4 cut(s) 977, 1517, 1602, 2072
Eco57I CTGAAG 2 cut(s) 445, 2051
Eco88I CYCGRG 2 cut(s) 639, 1512
EcoO109I RGGNCCY 1 cut(s) 1517
EcoRI GAATTC 5 cut(s) 55, 315, 381, 593, 2208
EcoRII CCWGG 6 cut(s) 286, 1010, 1068, 1201, 1586, 1864
EcoRV GATATC 1 cut(s) 1485
EcoT14I CCWWGG 2 cut(s) 1653, 1917
EcoT38I GRGCYC 1 cut(s) 1798
ErhI CCWWGG 2 cut(s) 1653, 1917
Esp3I CGTCTC 1 cut(s) 474
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FaqI GGGAC 3 cut(s) 1488, 1588, 1647
FauI CCCGC 1 cut(s) 718
FblI GTMKAC 2 cut(s) 1106, 1369
FokI GGATG 5 cut(s) 475, 917, 1126, 1358, 1689
FriOI GRGCYC 1 cut(s) 1798
FspBI CTAG 6 cut(s) 336, 869, 1305, 1338, 1469, 1757
GlaI GCGC 1 cut(s) 1312
GsaI CCCAGC 2 cut(s) 1191, 1956
GsuI CTGGAG 1 cut(s) 2195
HaeII RGCGCY 1 cut(s) 1314
HaeIII GGCC 4 cut(s) 445, 914, 1787, 1811
HapII CCGG 4 cut(s) 547, 1565, 1622, 1674
HgaI GACGC 1 cut(s) 491
HhaI GCGC 1 cut(s) 1313
Hin1I GRCGYC 1 cut(s) 483
Hin6I GCGC 1 cut(s) 1311
HinP1I GCGC 1 cut(s) 1311
HincII GTYRAC 1 cut(s) 1387
HindII GTYRAC 1 cut(s) 1387
HindIII AAGCTT 2 cut(s) 269, 457
HpaII CCGG 4 cut(s) 547, 1565, 1622, 1674
HphI GGTGA 3 cut(s) 143, 698, 2008
Hpy166II GTNNAC 6 cut(s) 94, 361, 563, 1107, 1370, 1387
Hpy188I TCNGA 8 cut(s) 145, 159, 454, 464, 599, 1174, 1894, 2231
Hpy188III TCNNGA 9 cut(s) 353, 492, 512, 1512, 1622, 1644, 1757, 2024, 2052
Hpy8I GTNNAC 6 cut(s) 94, 361, 563, 1107, 1370, 1387
HpyAV CCTTC 5 cut(s) 509, 1467, 1545, 2019, 2236
HpyCH4III ACNGT 9 cut(s) 567, 994, 1024, 1060, 1087, 1294, 2047, 2059, 2164
HpyCH4IV ACGT 1 cut(s) 1709
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 731, 752, 761, 1310, 1793
HpyF3I CTNAG 7 cut(s) 132, 144, 447, 605, 802, 1329, 1959
HpySE526I ACGT 1 cut(s) 1709
Hsp92I GRCGYC 1 cut(s) 483
HspAI GCGC 1 cut(s) 1311
Kpn2I TCCGGA 1 cut(s) 1621
Kzo9I GATC 4 cut(s) 1198, 1813, 1843, 1962
LguI GCTCTTC 1 cut(s) 1692
LmnI GCTCC 9 cut(s) 539, 751, 1012, 1191, 1424, 1567, 1801, 1867, 1919
Lsp1109I GCAGC 7 cut(s) 229, 634, 692, 782, 810, 1546, 1708
LweI GCATC 4 cut(s) 634, 820, 895, 1169
MaeI CTAG 6 cut(s) 336, 869, 1305, 1338, 1469, 1757
MaeII ACGT 1 cut(s) 1709
MaeIII GTNAC 5 cut(s) 542, 1294, 1741, 2059, 2185
MalI GATC 4 cut(s) 1200, 1815, 1845, 1964
MboI GATC 4 cut(s) 1198, 1813, 1843, 1962
MfeI CAATTG 1 cut(s) 1395
MhlI GDGCHC 4 cut(s) 565, 748, 1798, 1916
MlsI TGGCCA 1 cut(s) 914
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 5 cut(s) 154, 155, 403, 458, 1723
MmeI TCCRAC 2 cut(s) 323, 1730
Mox20I TGGCCA 1 cut(s) 914
MroI TCCGGA 1 cut(s) 1621
MroXI GAANNNNTTC 2 cut(s) 204, 385
MscI TGGCCA 1 cut(s) 914
Msp20I TGGCCA 1 cut(s) 914
MspA1I CMGCKG 1 cut(s) 683
MspCI CTTAAG 1 cut(s) 1544
MspI CCGG 4 cut(s) 547, 1565, 1622, 1674
MspR9I CCNGG 7 cut(s) 288, 1012, 1070, 1203, 1566, 1588, 1866
MunI CAATTG 1 cut(s) 1395
MvaI CCWGG 6 cut(s) 288, 1012, 1070, 1203, 1588, 1866
MwoI GCNNNNNNNGC 6 cut(s) 127, 731, 752, 761, 1310, 1793
NciI CCSGG 1 cut(s) 1566
NcoI CCATGG 2 cut(s) 1653, 1917
NdeII GATC 4 cut(s) 1198, 1813, 1843, 1962
NlaIV GGNNCC 4 cut(s) 28, 1519, 1604, 1797
NmeAIII GCCGAG 1 cut(s) 1813
NmuCI GTSAC 2 cut(s) 1294, 2059
NspI RCATGY 2 cut(s) 1971, 2261
NspV TTCGAA 1 cut(s) 2284
PagI TCATGA 1 cut(s) 2023
PaqCI CACCTGC 1 cut(s) 2105
PasI CCCWGGG 1 cut(s) 1069
PciSI GCTCTTC 1 cut(s) 1692
PdmI GAANNNNTTC 2 cut(s) 204, 385
PflFI GACNNNGTC 1 cut(s) 163
PfoI TCCNGGA 1 cut(s) 1201
PleI GAGTC 5 cut(s) 154, 154, 402, 457, 1722
PpsI GAGTC 5 cut(s) 154, 154, 402, 457, 1722
PpuMI RGGWCCY 1 cut(s) 1517
PshBI ATTAAT 1 cut(s) 1992
PsiI TTATAA 2 cut(s) 1926, 2105
Psp5II RGGWCCY 1 cut(s) 1517
Psp6I CCWGG 6 cut(s) 286, 1010, 1068, 1201, 1586, 1864
PspFI CCCAGC 2 cut(s) 1187, 1952
PspGI CCWGG 6 cut(s) 286, 1010, 1068, 1201, 1586, 1864
PspN4I GGNNCC 4 cut(s) 28, 1519, 1604, 1797
PspPI GGNCC 4 cut(s) 977, 1517, 1602, 2072
PspPPI RGGWCCY 1 cut(s) 1517
PstI CTGCAG 3 cut(s) 652, 800, 2116
PstNI CAGNNNCTG 1 cut(s) 163
PsyI GACNNNGTC 1 cut(s) 163
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 4 cut(s) 212, 610, 661, 1542
RsaNI GTAC 4 cut(s) 211, 609, 660, 1541
SapI GCTCTTC 1 cut(s) 1692
Sau3AI GATC 4 cut(s) 1198, 1813, 1843, 1962
Sau96I GGNCC 4 cut(s) 977, 1517, 1602, 2072
SbfI CCTGCAGG 1 cut(s) 2116
SchI GAGTC 5 cut(s) 154, 155, 403, 458, 1723
ScrFI CCNGG 7 cut(s) 288, 1012, 1070, 1203, 1566, 1588, 1866
SdaI CCTGCAGG 1 cut(s) 2116
SduI GDGCHC 4 cut(s) 565, 748, 1798, 1916
SfaNI GCATC 4 cut(s) 634, 820, 895, 1169
SfcI CTRYAG 4 cut(s) 50, 648, 796, 2112
SfuI TTCGAA 1 cut(s) 2284
SinI GGWCC 4 cut(s) 977, 1517, 1602, 2072
SmlI CTYRAG 1 cut(s) 1544
SmoI CTYRAG 1 cut(s) 1544
SpeI ACTAGT 1 cut(s) 1468
Sse8387I CCTGCAGG 1 cut(s) 2116
SsiI CCGC 5 cut(s) 192, 678, 725, 819, 1028
SspMI CTAG 6 cut(s) 336, 869, 1305, 1338, 1469, 1757
StyD4I CCNGG 7 cut(s) 286, 1010, 1068, 1201, 1564, 1586, 1864
StyI CCWWGG 2 cut(s) 1653, 1917
TaaI ACNGT 9 cut(s) 567, 994, 1024, 1060, 1087, 1294, 2047, 2059, 2164
TaiI ACGT 1 cut(s) 1712
TaqI TCGA 1 cut(s) 2284
TaqII GACCGA 1 cut(s) 1590
TatI WGTACW 2 cut(s) 210, 608
TauI GCSGC 3 cut(s) 194, 821, 1031
TscAI CASTG 3 cut(s) 439, 1027, 1189
TseFI GTSAC 2 cut(s) 1294, 2059
TseI GCWGC 7 cut(s) 242, 647, 680, 795, 798, 1559, 1721
Tsp45I GTSAC 2 cut(s) 1294, 2059
TspDTI ATGAA 7 cut(s) 287, 600, 1191, 1230, 1779, 1978, 2012
TspGWI ACGGA 2 cut(s) 1528, 1892
TspRI CASTG 3 cut(s) 439, 1027, 1189
Tth111I GACNNNGTC 1 cut(s) 163
Vha464I CTTAAG 1 cut(s) 1544
VneI GTGCAC 1 cut(s) 561
VpaK11BI GGWCC 4 cut(s) 977, 1517, 1602, 2072
VspI ATTAAT 1 cut(s) 1992
XapI RAATTY 9 cut(s) 55, 315, 346, 381, 438, 593, 970, 1000, 2208
XbaI TCTAGA 1 cut(s) 1756
XceI RCATGY 2 cut(s) 1971, 2261
XcmI CCANNNNNNNNNTGG 1 cut(s) 1945
XmiI GTMKAC 2 cut(s) 1106, 1369
XmnI GAANNNNTTC 2 cut(s) 204, 385
XspI CTAG 6 cut(s) 336, 869, 1305, 1338, 1469, 1757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.