Subtilase family
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_2g10210 FvH4_2g10210 FvH4_2g10210 |
| malus_domestica | MD05G1055900.v1.1 |
| prunus_persica | Prupe.8G086300_v2.0.a1 |
| pyrus_communis | pycom05g04760 |
| rosa_chinensis | RchiOBHm_Chr6g0267441 |
| rosa_laevigata | RLG00000014004 |
| rosa_multiflora | Rmu_sc0001068.1_g000015 Rmu_sc0006674.1_g000011 |
| rosa_roxburghii | Rroxscaffold_7G00200780 |
| rosa_rugosa | Rorug06G0032600 Rorug06G0032600 |
| rosa_samantha | Rh6AG154000 Rh6BG154100 Rh6CG149800 |
| rosa_wichuraiana | Rw6G013220 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2125 |
| Acc36I | ACCTGC | 1 cut(s) | 2125 |
| AccB1I | GGYRCC | 1 cut(s) | 1310 |
| AccI | GTMKAC | 3 cut(s) | 360, 1106, 1369 |
| AccII | CGCG | 1 cut(s) | 2106 |
| AciI | CCGC | 2 cut(s) | 878, 2204 |
| AclWI | GGATC | 1 cut(s) | 1814 |
| AcoI | YGGCCR | 1 cut(s) | 936 |
| AcsI | RAATTY | 4 cut(s) | 438, 593, 626, 1000 |
| AcuI | CTGAAG | 3 cut(s) | 633, 708, 1565 |
| AfaI | GTAC | 6 cut(s) | 212, 285, 661, 1219, 1542, 2246 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 14, 136, 1495 |
| AflIII | ACRYGT | 1 cut(s) | 1843 |
| AgsI | TTSAA | 8 cut(s) | 529, 626, 1382, 1646, 2091, 2111, 2161, 2188 |
| AjiI | CACGTC | 1 cut(s) | 853 |
| AjnI | CCWGG | 3 cut(s) | 13, 1068, 1564 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 579, 611 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1224, 1256 |
| Alw21I | GWGCWC | 2 cut(s) | 565, 748 |
| Alw26I | GTCTC | 6 cut(s) | 153, 456, 474, 1322, 1534, 2312 |
| Alw44I | GTGCAC | 1 cut(s) | 561 |
| AlwI | GGATC | 1 cut(s) | 1814 |
| Ama87I | CYCGRG | 3 cut(s) | 639, 1512, 1871 |
| AoxI | GGCC | 3 cut(s) | 443, 936, 976 |
| ApaLI | GTGCAC | 1 cut(s) | 561 |
| ApoI | RAATTY | 4 cut(s) | 438, 593, 626, 1000 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 204, 1127, 2227 |
| AspLEI | GCGC | 2 cut(s) | 1802, 1952 |
| AspS9I | GGNCC | 4 cut(s) | 977, 1517, 1590, 2078 |
| AsuC2I | CCSGG | 4 cut(s) | 288, 1189, 1872, 1873 |
| AsuHPI | GGTGA | 3 cut(s) | 19, 143, 2014 |
| AsuNHI | GCTAGC | 1 cut(s) | 1964 |
| AvaI | CYCGRG | 3 cut(s) | 639, 1512, 1871 |
| AvaII | GGWCC | 3 cut(s) | 1517, 1590, 2078 |
| BaeGI | GKGCMC | 1 cut(s) | 565 |
| BalI | TGGCCA | 1 cut(s) | 938 |
| BanI | GGYRCC | 1 cut(s) | 1310 |
| BanII | GRGCYC | 2 cut(s) | 1724, 1878 |
| BbsI | GAAGAC | 1 cut(s) | 1185 |
| Bbv12I | GWGCWC | 2 cut(s) | 565, 748 |
| BbvCI | CCTCAGC | 1 cut(s) | 1794 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1541, 1575 |
| BciT130I | CCWGG | 3 cut(s) | 15, 1070, 1566 |
| BciVI | GTATCC | 2 cut(s) | 778, 2268 |
| BcnI | CCSGG | 4 cut(s) | 288, 1189, 1872, 1873 |
| BcoDI | GTCTC | 6 cut(s) | 153, 456, 474, 1322, 1534, 2312 |
| BfaI | CTAG | 3 cut(s) | 336, 869, 1965 |
| BfmI | CTRYAG | 6 cut(s) | 50, 243, 678, 796, 1086, 1725 |
| BfoI | RGCGCY | 1 cut(s) | 1803 |
| BfuAI | ACCTGC | 1 cut(s) | 2125 |
| BfuI | GTATCC | 2 cut(s) | 778, 2268 |
| BlpI | GCTNAGC | 1 cut(s) | 802 |
| Bme1390I | CCNGG | 7 cut(s) | 15, 288, 1070, 1189, 1566, 1872, 1873 |
| Bme18I | GGWCC | 3 cut(s) | 1517, 1590, 2078 |
| BmeT110I | CYCGRG | 3 cut(s) | 639, 1512, 1871 |
| BmgBI | CACGTC | 1 cut(s) | 853 |
| BmgT120I | GGNCC | 4 cut(s) | 977, 1517, 1590, 2078 |
| BmiI | GGNNCC | 6 cut(s) | 6, 65, 1312, 1519, 1592, 1721 |
| BmrFI | CCNGG | 7 cut(s) | 15, 288, 1070, 1189, 1566, 1872, 1873 |
| BmrI | ACTGGG | 1 cut(s) | 2257 |
| BmsI | GCATC | 6 cut(s) | 634, 820, 1159, 1169, 1713, 1977 |
| BmtI | GCTAGC | 1 cut(s) | 1968 |
| BmuI | ACTGGG | 1 cut(s) | 2257 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1991 |
| BpiI | GAAGAC | 1 cut(s) | 1185 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2263, 2295 |
| BpmI | CTGGAG | 2 cut(s) | 1611, 2201 |
| Bpu10I | CCTNAGC | 2 cut(s) | 132, 1794 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 802 |
| BpuEI | CTTGAG | 2 cut(s) | 246, 2021 |
| BpuMI | CCSGG | 4 cut(s) | 288, 1189, 1872, 1873 |
| BsaI | GGTCTC | 1 cut(s) | 1322 |
| BsaJI | CCNNGG | 8 cut(s) | 14, 1010, 1068, 1069, 1359, 1870, 1871, 1923 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 14, 136, 1495 |
| Bse118I | RCCGGY | 3 cut(s) | 683, 1313, 2120 |
| Bse1I | ACTGG | 6 cut(s) | 439, 793, 1628, 2081, 2184, 2252 |
| Bse3DI | GCAATG | 3 cut(s) | 538, 1155, 2097 |
| BseBI | CCWGG | 3 cut(s) | 15, 1070, 1566 |
| BseDI | CCNNGG | 8 cut(s) | 14, 1010, 1068, 1069, 1359, 1870, 1871, 1923 |
| BseGI | GGATG | 8 cut(s) | 671, 1119, 1123, 1271, 1351, 1704, 1905, 2052 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 14, 136, 1495 |
| BseMI | GCAATG | 3 cut(s) | 538, 1155, 2097 |
| BseMII | CTCAG | 9 cut(s) | 123, 135, 438, 816, 963, 1343, 1785, 1820, 1911 |
| BseNI | ACTGG | 6 cut(s) | 439, 793, 1628, 2081, 2184, 2252 |
| BseRI | GAGGAG | 2 cut(s) | 753, 1794 |
| BseSI | GKGCMC | 1 cut(s) | 565 |
| BseYI | CCCAGC | 2 cut(s) | 1304, 1337 |
| BsgI | GTGCAG | 1 cut(s) | 1266 |
| Bsh1236I | CGCG | 1 cut(s) | 2106 |
| BshFI | GGCC | 3 cut(s) | 445, 938, 978 |
| BshNI | GGYRCC | 1 cut(s) | 1310 |
| BsiHKAI | GWGCWC | 2 cut(s) | 565, 748 |
| BsiHKCI | CYCGRG | 3 cut(s) | 639, 1512, 1871 |
| BsiSI | CCGG | 6 cut(s) | 288, 684, 1188, 1314, 1872, 2121 |
| BslFI | GGGAC | 2 cut(s) | 729, 1576 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 14, 136, 1495 |
| BsmAI | GTCTC | 6 cut(s) | 153, 456, 474, 1322, 1534, 2312 |
| BsmFI | GGGAC | 2 cut(s) | 729, 1576 |
| BsmI | GAATGC | 1 cut(s) | 1499 |
| BsnI | GGCC | 3 cut(s) | 445, 938, 978 |
| Bso31I | GGTCTC | 1 cut(s) | 1322 |
| BsoBI | CYCGRG | 3 cut(s) | 639, 1512, 1871 |
| Bsp1286I | GDGCHC | 4 cut(s) | 565, 748, 1724, 1878 |
| Bsp1407I | TGTACA | 1 cut(s) | 210 |
| Bsp143I | GATC | 2 cut(s) | 1768, 1819 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 802 |
| Bsp19I | CCATGG | 1 cut(s) | 1923 |
| BspACI | CCGC | 2 cut(s) | 878, 2204 |
| BspANI | GGCC | 3 cut(s) | 445, 938, 978 |
| BspCNI | CTCAG | 9 cut(s) | 124, 136, 439, 815, 962, 1342, 1786, 1819, 1910 |
| BspFNI | CGCG | 1 cut(s) | 2106 |
| BspLI | GGNNCC | 6 cut(s) | 6, 65, 1312, 1519, 1592, 1721 |
| BspMAI | CTGCAG | 5 cut(s) | 54, 247, 682, 800, 1729 |
| BspMI | ACCTGC | 1 cut(s) | 2125 |
| BspOI | GCTAGC | 1 cut(s) | 1968 |
| BspPI | GGATC | 1 cut(s) | 1814 |
| BspQI | GCTCTTC | 1 cut(s) | 1698 |
| BspT107I | GGYRCC | 1 cut(s) | 1310 |
| BspTNI | GGTCTC | 1 cut(s) | 1322 |
| BsrDI | GCAATG | 3 cut(s) | 538, 1155, 2097 |
| BsrFI | RCCGGY | 3 cut(s) | 683, 1313, 2120 |
| BsrGI | TGTACA | 1 cut(s) | 210 |
| BsrI | ACTGG | 6 cut(s) | 439, 793, 1628, 2081, 2184, 2252 |
| BssAI | RCCGGY | 3 cut(s) | 683, 1313, 2120 |
| BssECI | CCNNGG | 8 cut(s) | 14, 1010, 1068, 1069, 1359, 1870, 1871, 1923 |
| BssMI | GATC | 2 cut(s) | 1768, 1819 |
| BssNAI | GTATAC | 1 cut(s) | 1107 |
| BssT1I | CCWWGG | 2 cut(s) | 1010, 1923 |
| Bst1107I | GTATAC | 1 cut(s) | 1107 |
| Bst2UI | CCWGG | 3 cut(s) | 15, 1070, 1566 |
| Bst6I | CTCTTC | 1 cut(s) | 1698 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1974 |
| BstAUI | TGTACA | 1 cut(s) | 210 |
| BstC8I | GCNNGC | 6 cut(s) | 565, 706, 1033, 1966, 2104, 2122 |
| BstDEI | CTNAG | 9 cut(s) | 132, 144, 447, 802, 949, 1329, 1794, 1806, 1897 |
| BstDSI | CCRYGG | 1 cut(s) | 1923 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 12 |
| BstF5I | GGATG | 8 cut(s) | 671, 1119, 1123, 1271, 1351, 1704, 1905, 2052 |
| BstFNI | CGCG | 1 cut(s) | 2106 |
| BstH2I | RGCGCY | 1 cut(s) | 1803 |
| BstHHI | GCGC | 2 cut(s) | 1802, 1952 |
| BstKTI | GATC | 2 cut(s) | 1771, 1822 |
| BstMAI | GTCTC | 6 cut(s) | 153, 456, 474, 1322, 1534, 2312 |
| BstMBI | GATC | 2 cut(s) | 1768, 1819 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 1310, 1799, 1949, 1974 |
| BstNI | CCWGG | 3 cut(s) | 15, 1070, 1566 |
| BstNSI | RCATGY | 2 cut(s) | 1847, 1977 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1991 |
| BstSCI | CCNGG | 7 cut(s) | 13, 286, 1068, 1187, 1564, 1870, 1871 |
| BstSFI | CTRYAG | 6 cut(s) | 50, 243, 678, 796, 1086, 1725 |
| BstSLI | GKGCMC | 1 cut(s) | 565 |
| BstUI | CGCG | 1 cut(s) | 2106 |
| BstV2I | GAAGAC | 1 cut(s) | 1185 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 495, 756 |
| BstZ17I | GTATAC | 1 cut(s) | 1107 |
| BsuI | GTATCC | 2 cut(s) | 778, 2268 |
| BsuRI | GGCC | 3 cut(s) | 445, 938, 978 |
| BtgI | CCRYGG | 1 cut(s) | 1923 |
| BtrI | CACGTC | 1 cut(s) | 853 |
| BtsCI | GGATG | 8 cut(s) | 671, 1119, 1123, 1271, 1351, 1704, 1905, 2052 |
| BtsI | GCAGTG | 2 cut(s) | 675, 1275 |
| BtsIMutI | CAGTG | 6 cut(s) | 432, 675, 1020, 1189, 1275, 2292 |
| BveI | ACCTGC | 1 cut(s) | 2125 |
| Cac8I | GCNNGC | 6 cut(s) | 565, 706, 1033, 1966, 2104, 2122 |
| CfoI | GCGC | 2 cut(s) | 1802, 1952 |
| Cfr10I | RCCGGY | 3 cut(s) | 683, 1313, 2120 |
| Cfr13I | GGNCC | 4 cut(s) | 977, 1517, 1590, 2078 |
| Cfr9I | CCCGGG | 1 cut(s) | 1871 |
| Csp6I | GTAC | 6 cut(s) | 211, 284, 660, 1218, 1541, 2245 |
| CviQI | GTAC | 6 cut(s) | 211, 284, 660, 1218, 1541, 2245 |
| DdeI | CTNAG | 9 cut(s) | 132, 144, 447, 802, 949, 1329, 1794, 1806, 1897 |
| DpnI | GATC | 2 cut(s) | 1770, 1821 |
| DpnII | GATC | 2 cut(s) | 1768, 1819 |
| DraI | TTTAAA | 1 cut(s) | 1639 |
| EaeI | YGGCCR | 1 cut(s) | 936 |
| Eam1104I | CTCTTC | 1 cut(s) | 1698 |
| EarI | CTCTTC | 1 cut(s) | 1698 |
| EciI | GGCGGA | 1 cut(s) | 867 |
| Eco130I | CCWWGG | 2 cut(s) | 1010, 1923 |
| Eco24I | GRGCYC | 2 cut(s) | 1724, 1878 |
| Eco31I | GGTCTC | 1 cut(s) | 1322 |
| Eco32I | GATATC | 1 cut(s) | 1485 |
| Eco47I | GGWCC | 3 cut(s) | 1517, 1590, 2078 |
| Eco57I | CTGAAG | 3 cut(s) | 633, 708, 1565 |
| Eco88I | CYCGRG | 3 cut(s) | 639, 1512, 1871 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 12 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1517 |
| EcoRII | CCWGG | 3 cut(s) | 13, 1068, 1564 |
| EcoRV | GATATC | 1 cut(s) | 1485 |
| EcoT14I | CCWWGG | 2 cut(s) | 1010, 1923 |
| EcoT38I | GRGCYC | 2 cut(s) | 1724, 1878 |
| ErhI | CCWWGG | 2 cut(s) | 1010, 1923 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2215, 2247 |
| FaqI | GGGAC | 2 cut(s) | 729, 1576 |
| FauNDI | CATATG | 2 cut(s) | 169, 506 |
| FblI | GTMKAC | 3 cut(s) | 360, 1106, 1369 |
| FokI | GGATG | 8 cut(s) | 658, 1126, 1130, 1258, 1358, 1691, 1892, 2059 |
| FriOI | GRGCYC | 2 cut(s) | 1724, 1878 |
| FspBI | CTAG | 3 cut(s) | 336, 869, 1965 |
| GlaI | GCGC | 2 cut(s) | 1801, 1951 |
| GsaI | CCCAGC | 2 cut(s) | 1308, 1341 |
| GsuI | CTGGAG | 2 cut(s) | 1611, 2201 |
| HaeII | RGCGCY | 1 cut(s) | 1803 |
| HaeIII | GGCC | 3 cut(s) | 445, 938, 978 |
| HapII | CCGG | 6 cut(s) | 288, 684, 1188, 1314, 1872, 2121 |
| HhaI | GCGC | 2 cut(s) | 1802, 1952 |
| Hin6I | GCGC | 2 cut(s) | 1800, 1950 |
| HinP1I | GCGC | 2 cut(s) | 1800, 1950 |
| HindIII | AAGCTT | 2 cut(s) | 457, 706 |
| HinfI | GANTC | 9 cut(s) | 146, 160, 200, 362, 394, 423, 449, 945, 1646 |
| HpaII | CCGG | 6 cut(s) | 288, 684, 1188, 1314, 1872, 2121 |
| HphI | GGTGA | 3 cut(s) | 19, 143, 2014 |
| Hpy188I | TCNGA | 4 cut(s) | 145, 1363, 1809, 1900 |
| Hpy188III | TCNNGA | 6 cut(s) | 320, 512, 651, 726, 1650, 2058 |
| HpyAV | CCTTC | 4 cut(s) | 77, 509, 1545, 2025 |
| HpyCH4IV | ACGT | 1 cut(s) | 852 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 1310, 1799, 1949, 1974 |
| HpyF3I | CTNAG | 9 cut(s) | 132, 144, 447, 802, 949, 1329, 1794, 1806, 1897 |
| HpySE526I | ACGT | 1 cut(s) | 852 |
| HspAI | GCGC | 2 cut(s) | 1800, 1950 |
| KroI | GCCGGC | 1 cut(s) | 2120 |
| KroNI | GCCGGC | 1 cut(s) | 2122 |
| Kzo9I | GATC | 2 cut(s) | 1768, 1819 |
| LguI | GCTCTTC | 1 cut(s) | 1698 |
| LweI | GCATC | 6 cut(s) | 634, 820, 1159, 1169, 1713, 1977 |
| MaeI | CTAG | 3 cut(s) | 336, 869, 1965 |
| MaeII | ACGT | 1 cut(s) | 852 |
| MaeIII | GTNAC | 2 cut(s) | 25, 2065 |
| MalI | GATC | 2 cut(s) | 1770, 1821 |
| MboI | GATC | 2 cut(s) | 1768, 1819 |
| MboII | GAAGA | 9 cut(s) | 32, 562, 1190, 1495, 1685, 1778, 1822, 2235, 2264 |
| MhlI | GDGCHC | 4 cut(s) | 565, 748, 1724, 1878 |
| MlsI | TGGCCA | 1 cut(s) | 938 |
| MluNI | TGGCCA | 1 cut(s) | 938 |
| MlyI | GAGTC | 5 cut(s) | 155, 356, 403, 432, 458 |
| MmeI | TCCRAC | 2 cut(s) | 323, 476 |
| Mox20I | TGGCCA | 1 cut(s) | 938 |
| MroNI | GCCGGC | 1 cut(s) | 2120 |
| MroXI | GAANNNNTTC | 3 cut(s) | 204, 1127, 2227 |
| MscI | TGGCCA | 1 cut(s) | 938 |
| MseI | TTAA | 8 cut(s) | 576, 1451, 1638, 1698, 2001, 2085, 2133, 2139 |
| Msp20I | TGGCCA | 1 cut(s) | 938 |
| MspA1I | CMGCKG | 1 cut(s) | 2206 |
| MspI | CCGG | 6 cut(s) | 288, 684, 1188, 1314, 1872, 2121 |
| MspR9I | CCNGG | 7 cut(s) | 15, 288, 1070, 1189, 1566, 1872, 1873 |
| Mva1269I | GAATGC | 1 cut(s) | 1499 |
| MvaI | CCWGG | 3 cut(s) | 15, 1070, 1566 |
| MvnI | CGCG | 1 cut(s) | 2106 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 1310, 1799, 1949, 1974 |
| NaeI | GCCGGC | 1 cut(s) | 2122 |
| NciI | CCSGG | 4 cut(s) | 288, 1189, 1872, 1873 |
| NcoI | CCATGG | 1 cut(s) | 1923 |
| NdeI | CATATG | 2 cut(s) | 169, 506 |
| NdeII | GATC | 2 cut(s) | 1768, 1819 |
| NgoMIV | GCCGGC | 1 cut(s) | 2120 |
| NheI | GCTAGC | 1 cut(s) | 1964 |
| NlaIV | GGNNCC | 6 cut(s) | 6, 65, 1312, 1519, 1592, 1721 |
| NmuCI | GTSAC | 2 cut(s) | 25, 2065 |
| NspI | RCATGY | 2 cut(s) | 1847, 1977 |
| PaeR7I | CTCGAG | 1 cut(s) | 1512 |
| PaqCI | CACCTGC | 1 cut(s) | 2125 |
| PasI | CCCWGGG | 1 cut(s) | 1069 |
| PciI | ACATGT | 1 cut(s) | 1843 |
| PciSI | GCTCTTC | 1 cut(s) | 1698 |
| PctI | GAATGC | 1 cut(s) | 1499 |
| PdiI | GCCGGC | 1 cut(s) | 2122 |
| PdmI | GAANNNNTTC | 3 cut(s) | 204, 1127, 2227 |
| PfeI | GAWTC | 4 cut(s) | 160, 200, 945, 1646 |
| PleI | GAGTC | 5 cut(s) | 154, 356, 402, 431, 457 |
| PpsI | GAGTC | 5 cut(s) | 154, 356, 402, 431, 457 |
| PpuMI | RGGWCCY | 1 cut(s) | 1517 |
| PscI | ACATGT | 1 cut(s) | 1843 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1991 |
| Psp5II | RGGWCCY | 1 cut(s) | 1517 |
| Psp6I | CCWGG | 3 cut(s) | 13, 1068, 1564 |
| PspFI | CCCAGC | 2 cut(s) | 1304, 1337 |
| PspGI | CCWGG | 3 cut(s) | 13, 1068, 1564 |
| PspN4I | GGNNCC | 6 cut(s) | 6, 65, 1312, 1519, 1592, 1721 |
| PspPI | GGNCC | 4 cut(s) | 977, 1517, 1590, 2078 |
| PspPPI | RGGWCCY | 1 cut(s) | 1517 |
| PspXI | VCTCGAGB | 1 cut(s) | 1512 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1115, 1147 |
| PstI | CTGCAG | 5 cut(s) | 54, 247, 682, 800, 1729 |
| RsaI | GTAC | 6 cut(s) | 212, 285, 661, 1219, 1542, 2246 |
| RsaNI | GTAC | 6 cut(s) | 211, 284, 660, 1218, 1541, 2245 |
| SapI | GCTCTTC | 1 cut(s) | 1698 |
| SaqAI | TTAA | 8 cut(s) | 576, 1451, 1638, 1698, 2001, 2085, 2133, 2139 |
| Sau3AI | GATC | 2 cut(s) | 1768, 1819 |
| Sau96I | GGNCC | 4 cut(s) | 977, 1517, 1590, 2078 |
| SchI | GAGTC | 5 cut(s) | 155, 356, 403, 432, 458 |
| ScrFI | CCNGG | 7 cut(s) | 15, 288, 1070, 1189, 1566, 1872, 1873 |
| SduI | GDGCHC | 4 cut(s) | 565, 748, 1724, 1878 |
| SfaNI | GCATC | 6 cut(s) | 634, 820, 1159, 1169, 1713, 1977 |
| SfcI | CTRYAG | 6 cut(s) | 50, 243, 678, 796, 1086, 1725 |
| Sfr274I | CTCGAG | 1 cut(s) | 1512 |
| SinI | GGWCC | 3 cut(s) | 1517, 1590, 2078 |
| SlaI | CTCGAG | 1 cut(s) | 1512 |
| SmaI | CCCGGG | 1 cut(s) | 1873 |
| SmlI | CTYRAG | 3 cut(s) | 261, 1512, 2036 |
| SmoI | CTYRAG | 3 cut(s) | 261, 1512, 2036 |
| SsiI | CCGC | 2 cut(s) | 878, 2204 |
| SspMI | CTAG | 3 cut(s) | 336, 869, 1965 |
| StyD4I | CCNGG | 7 cut(s) | 13, 286, 1068, 1187, 1564, 1870, 1871 |
| StyI | CCWWGG | 2 cut(s) | 1010, 1923 |
| TaiI | ACGT | 1 cut(s) | 855 |
| TaqI | TCGA | 5 cut(s) | 1041, 1513, 1788, 1827, 1985 |
| TatI | WGTACW | 2 cut(s) | 210, 2244 |
| TfiI | GAWTC | 4 cut(s) | 160, 200, 945, 1646 |
| Tru1I | TTAA | 8 cut(s) | 576, 1451, 1638, 1698, 2001, 2085, 2133, 2139 |
| Tru9I | TTAA | 8 cut(s) | 576, 1451, 1638, 1698, 2001, 2085, 2133, 2139 |
| TscAI | CASTG | 6 cut(s) | 439, 682, 1027, 1189, 1282, 2292 |
| TseFI | GTSAC | 2 cut(s) | 25, 2065 |
| Tsp45I | GTSAC | 2 cut(s) | 25, 2065 |
| TspDTI | ATGAA | 5 cut(s) | 600, 917, 1191, 2018, 2252 |
| TspGWI | ACGGA | 1 cut(s) | 1898 |
| TspMI | CCCGGG | 1 cut(s) | 1871 |
| TspRI | CASTG | 6 cut(s) | 439, 682, 1027, 1189, 1282, 2292 |
| VneI | GTGCAC | 1 cut(s) | 561 |
| VpaK11BI | GGWCC | 3 cut(s) | 1517, 1590, 2078 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 12 |
| XapI | RAATTY | 4 cut(s) | 438, 593, 626, 1000 |
| XceI | RCATGY | 2 cut(s) | 1847, 1977 |
| XhoI | CTCGAG | 1 cut(s) | 1512 |
| XmaI | CCCGGG | 1 cut(s) | 1871 |
| XmiI | GTMKAC | 3 cut(s) | 360, 1106, 1369 |
| XmnI | GAANNNNTTC | 3 cut(s) | 204, 1127, 2227 |
| XspI | CTAG | 3 cut(s) | 336, 869, 1965 |