Rh6CG149800

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0014653
Physical Location & Seq
Forward (+)
19556915 .. 19563340
6426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG149800.1

Sequence Viewer

Length: 2379 bp
ATGTATTTGTCTTCAATTTCAGGCCGACTGAAGGACACACTCTCACTCACTCATATGGCTCCTCCTCCAGGGATTGCTGTCACCCTTTTGCTTCTTCTTGCTCTGCAGAGTTATGGGTTCCTTCTCTCTTTTGCTTCCACAAATGTTTACATTGTTTATATGGGAGAAAGAAAGTATGCAAAGCCTGAGCTGGTATCTGAGTCTCATCATGAGATTCTGTCATATGTATTTGGAAGCAAAGAAGCCGCAAGGGAATCAATTCTGTACACCTACAAGCATGGATTTTCGGGGTTCGCTGCAGTTCTAACTCCATCTCAAGCCAAGCATATTGCAGATGTACCCGGTGTTGTTCGTGTCATTCCTCACAGGTTTCTTGATTTGCATACAACTAGAAGTTGGAACTTTCTGCAAGTAGACTCTCACTTTAGAAATGGAGTTCTTTCAAGGAGTCAATCAGGCGTTGGGTCTATTATTGGAGTCATGGACACTGGAATTTGGCCCGAGTCTCAAAGCTTTAGAGATGAGGGTATGGGAGACATTCCATCTCGTTGGAGAGGCATATGTCAAGAAGGAGAAAGGTTCAATCACTCCCATTGCAACAGGAAGATTATTGGTGCACGCTGGTATATTAAAGGATATGAAGCTGAATTTGGAAAGATAGATACAAGTGATGGGTTTGAATTTCTATCTCCCCGAGATGCTTCAGGACATGGTACTCACACATCATCCACTGCAGCCGGTGGTTTTATAGAAAATGCAAGCTTTATGGGACTTGCTTCAGGATTGGCAAGAGGAGGTGCTCCATCATCTTGGTTAGCTATTTACAAAGTTTGTTGGGCTACTGGTGGCTGCAGCTCAGCAGACATACTTGCTGCATTTGATGATGCAATTTTTGATGGTGTGGACGTGCTTTCGGCATCTCTAGGTTCTCCGCCTCCACTTGCTTCTTATGTTGAGGATGTTGTGTCCATTGGTTCTTTTCATGCTGTGGCCAAAGGAATCTCAGTTGTATGCTCTGCTGGAAACTCTGGCCCATACTCTCAAACTATCATAAATTCTGCTCCTTGGATATTCACTGTTGCAGCAAGCACTATCGACAGGGCTTTTCCTACTGCAATCTCCCTGGGAAACAATCAAACTGTAGTGGGTCAGGGTTTGTATACAGGGATGGATGTGAACAAGTTCTACCCTCTTGTGTATGGACAAGACATTGCATCTGTTGATGCAGACGAAGACAGTGCCGGGAGCTGTGAGTTGGGAACTCTAAATGGTACTTTAGCAAACGGAAAATTTGTTCTTTGTTTCCAATCTCGATCACAGAGGTCATCCACTGCTGCACAGAGAACTGTAATGAGTGCTGGGGGTGCCGGTCTCATTTTTGCTCAGTTTCCCAGCAAGGATGTTTCTTTGTCCTCGGATAGTCTACCGTGTGTTCAAATTGACTTTGCAATCGGCACATATATTCTCACATATATTGGAGCAACCAGAAATCCAATAGTCAAGTTAAACCCTACAAAAACAGTATTGGAACAGCAGATATCCCCAGAAGTGGCATTCTTCTCTGCTCGAGGACCCAATTCTGTCTCTCCTTCGGTACTGAAGCCCGATATTGCTGCTCCTGGTGTGAATATATTGTCCTCTTGGTCCCCTGCTTATTCACTATTACAACATCAACCATCTCCAGTCATCTTTAAAATTGAATCAGGAACTTCCATGGCTTGTCCACATATATCTGGTATAGTGGCTCTTCTTAAAAGCATCCACCCAATATGGAGCCCTGCAGCAATAAAGTCTGCCCTTATTACAACAGCCTCTTTAGAAGATCAATACGGTCAAAGTATCGAGGCTGAGGGCGCTCCTCAGAAGAAAGCTGATCCATTCGACTATGGTGGTGGACATGTCAATCCTAACAAAGCCATAGCCCCCGGGCTCATTTATGATATAGAAACCTCAGATTACATCCGTTTTCTTTGCTCCATGGGATATAACAATTCAGCCATCAGCGCAATCGCAGGAGCTAGCATCACATGCCATAAATCGACCAATGTCCAAGTTAATCTCAACCTGCCTTCCATCACCATTCATGAGCTCAAGGAAAGGATGACAGTATCAAGAACAGTCACAAATGTTGGTCCAGTTAATTCTATCTACATTGCTCGCGTTCAAACACCTGCCGGCGTGTCTATTAAAGTTAAGCCATCGGCTTTGTTGTTCAACTCTACTGCAAAGAAGCTGGAGTTCAAGGTTGTTTTTCGTCCCCTGCTAAAAGTTGCAGGAAGATTTTCTTTTGGAAATCTGTACTGGGAAGATGGTTTTCATGTAGTGAGGATACCTCTAACAGTGAGAACTGTCCTTGATGATTTTTACGCAGAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

792

Amino Acids

84.9

Weight (kDa)

6.85

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 49 - 128 6.8e-18 Peptidase inhibitor I9
MORF_dom PF21864 52 - 127 1e-05 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 155 - 609 6.2e-44 Subtilase family
fn3_6 PF17766 686 - 781 1e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014653)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10210 FvH4_2g10210 FvH4_2g10210
malus_domestica MD05G1055900.v1.1
prunus_persica Prupe.8G086300_v2.0.a1
pyrus_communis pycom05g04760
rosa_chinensis RchiOBHm_Chr6g0267441
rosa_laevigata RLG00000014004
rosa_multiflora Rmu_sc0001068.1_g000015 Rmu_sc0006674.1_g000011
rosa_roxburghii Rroxscaffold_7G00200780
rosa_rugosa Rorug06G0032600 Rorug06G0032600
rosa_samantha Rh6AG154000 Rh6BG154100 Rh6CG149800
rosa_wichuraiana Rw6G013220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2179
Acc36I ACCTGC 2 cut(s) 2073, 2179
AccB1I GGYRCC 1 cut(s) 1364
AccI GTMKAC 3 cut(s) 414, 1160, 1423
AccII CGCG 1 cut(s) 2160
AciI CCGC 2 cut(s) 246, 932
AclWI GGATC 1 cut(s) 1868
AcoI YGGCCR 1 cut(s) 990
AcsI RAATTY 5 cut(s) 492, 647, 680, 1054, 1289
AcuI CTGAAG 4 cut(s) 50, 687, 762, 1619
AfaI GTAC 6 cut(s) 266, 339, 715, 1273, 1596, 2300
AfiI CCNNNNNNNGG 3 cut(s) 31, 68, 1549
AflIII ACRYGT 1 cut(s) 1897
AgsI TTSAA 9 cut(s) 15, 444, 583, 680, 1436, 1700, 2165, 2215, 2242
AjiI CACGTC 1 cut(s) 907
AjnI CCWGG 3 cut(s) 67, 1122, 1618
AjuI GAANNNNNNNTTGG 2 cut(s) 633, 665
AloI GAACNNNNNNTCC 2 cut(s) 1278, 1310
Alw21I GWGCWC 3 cut(s) 619, 802, 2091
Alw26I GTCTC 5 cut(s) 207, 510, 528, 1376, 1588
Alw44I GTGCAC 1 cut(s) 615
AlwI GGATC 1 cut(s) 1868
Ama87I CYCGRG 4 cut(s) 500, 693, 1566, 1925
AoxI GGCC 4 cut(s) 22, 497, 990, 1030
ApaLI GTGCAC 1 cut(s) 615
ApeKI GCWGC 9 cut(s) 296, 734, 849, 852, 872, 1082, 1334, 1613, 1781
ApoI RAATTY 5 cut(s) 492, 647, 680, 1054, 1289
Asp700I GAANNNNTTC 3 cut(s) 258, 1181, 2281
AspLEI GCGC 2 cut(s) 1856, 2006
AspS9I GGNCC 5 cut(s) 498, 1031, 1571, 1644, 2132
AsuC2I CCSGG 4 cut(s) 342, 1243, 1926, 1927
AsuHPI GGTGA 2 cut(s) 73, 2068
AsuNHI GCTAGC 1 cut(s) 2018
AvaI CYCGRG 4 cut(s) 500, 693, 1566, 1925
AvaII GGWCC 3 cut(s) 1571, 1644, 2132
BaeGI GKGCMC 1 cut(s) 619
BalI TGGCCA 1 cut(s) 992
BanI GGYRCC 1 cut(s) 1364
BanII GRGCYC 3 cut(s) 1778, 1932, 2091
BbsI GAAGAC 2 cut(s) 3, 1239
Bbv12I GWGCWC 3 cut(s) 619, 802, 2091
BbvCI CCTCAGC 1 cut(s) 1848
BbvI GCAGC 9 cut(s) 283, 746, 836, 859, 864, 1094, 1321, 1600, 1793
BcgI CGANNNNNNTGC 4 cut(s) 1220, 1254, 1595, 1629
BciT130I CCWGG 3 cut(s) 69, 1124, 1620
BciVI GTATCC 1 cut(s) 2322
BcnI CCSGG 4 cut(s) 342, 1243, 1926, 1927
BcoDI GTCTC 5 cut(s) 207, 510, 528, 1376, 1588
BfaI CTAG 3 cut(s) 390, 923, 2019
BfmI CTRYAG 6 cut(s) 104, 297, 732, 850, 1140, 1779
BfoI RGCGCY 1 cut(s) 1857
BfuAI ACCTGC 2 cut(s) 2073, 2179
BfuI GTATCC 1 cut(s) 2322
BlpI GCTNAGC 1 cut(s) 856
Bme1390I CCNGG 7 cut(s) 69, 342, 1124, 1243, 1620, 1926, 1927
Bme18I GGWCC 3 cut(s) 1571, 1644, 2132
BmeT110I CYCGRG 4 cut(s) 500, 693, 1566, 1925
BmgBI CACGTC 1 cut(s) 907
BmgT120I GGNCC 5 cut(s) 498, 1031, 1571, 1644, 2132
BmiI GGNNCC 6 cut(s) 60, 119, 1366, 1573, 1646, 1775
BmrFI CCNGG 7 cut(s) 69, 342, 1124, 1243, 1620, 1926, 1927
BmrI ACTGGG 1 cut(s) 2311
BmsI GCATC 7 cut(s) 688, 874, 926, 1213, 1223, 1767, 2031
BmtI GCTAGC 1 cut(s) 2022
BmuI ACTGGG 1 cut(s) 2311
BoxI GACNNNNGTC 1 cut(s) 2045
BpiI GAAGAC 2 cut(s) 3, 1239
BplI GAGNNNNNCTC 2 cut(s) 2317, 2349
BpmI CTGGAG 3 cut(s) 51, 1665, 2255
Bpu10I CCTNAGC 2 cut(s) 186, 1848
Bpu1102I GCTNAGC 1 cut(s) 856
BpuEI CTTGAG 2 cut(s) 300, 2075
BpuMI CCSGG 4 cut(s) 342, 1243, 1926, 1927
BsaI GGTCTC 1 cut(s) 1376
BsaJI CCNNGG 9 cut(s) 68, 1064, 1122, 1123, 1413, 1713, 1924, 1925, 1977
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 68, 1549
Bse118I RCCGGY 3 cut(s) 737, 1367, 2174
Bse1I ACTGG 5 cut(s) 493, 847, 1682, 2135, 2306
Bse3DI GCAATG 3 cut(s) 592, 1209, 2151
BseBI CCWGG 3 cut(s) 69, 1124, 1620
BseDI CCNNGG 9 cut(s) 68, 1064, 1122, 1123, 1413, 1713, 1924, 1925, 1977
BseGI GGATG 9 cut(s) 725, 964, 1173, 1177, 1325, 1405, 1758, 1959, 2106
BseLI CCNNNNNNNGG 3 cut(s) 31, 68, 1549
BseMI GCAATG 3 cut(s) 592, 1209, 2151
BseMII CTCAG 8 cut(s) 177, 189, 870, 1017, 1397, 1839, 1874, 1965
BseNI ACTGG 5 cut(s) 493, 847, 1682, 2135, 2306
BseRI GAGGAG 4 cut(s) 51, 54, 807, 1848
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 9 cut(s) 283, 746, 836, 859, 864, 1094, 1321, 1600, 1793
BseYI CCCAGC 2 cut(s) 1358, 1391
BsgI GTGCAG 1 cut(s) 1320
Bsh1236I CGCG 1 cut(s) 2160
BshFI GGCC 4 cut(s) 24, 499, 992, 1032
BshNI GGYRCC 1 cut(s) 1364
BsiHKAI GWGCWC 3 cut(s) 619, 802, 2091
BsiHKCI CYCGRG 4 cut(s) 500, 693, 1566, 1925
BsiSI CCGG 6 cut(s) 342, 738, 1242, 1368, 1926, 2175
BslFI GGGAC 3 cut(s) 783, 1630, 2241
BslI CCNNNNNNNGG 3 cut(s) 31, 68, 1549
BsmAI GTCTC 5 cut(s) 207, 510, 528, 1376, 1588
BsmFI GGGAC 3 cut(s) 783, 1630, 2241
BsmI GAATGC 1 cut(s) 1553
BsnI GGCC 4 cut(s) 24, 499, 992, 1032
Bso31I GGTCTC 1 cut(s) 1376
BsoBI CYCGRG 4 cut(s) 500, 693, 1566, 1925
Bsp1286I GDGCHC 5 cut(s) 619, 802, 1778, 1932, 2091
Bsp1407I TGTACA 1 cut(s) 264
Bsp143I GATC 3 cut(s) 1313, 1822, 1873
Bsp1720I GCTNAGC 1 cut(s) 856
Bsp19I CCATGG 2 cut(s) 1713, 1977
BspACI CCGC 2 cut(s) 246, 932
BspANI GGCC 4 cut(s) 24, 499, 992, 1032
BspCNI CTCAG 8 cut(s) 178, 190, 869, 1016, 1396, 1840, 1873, 1964
BspFNI CGCG 1 cut(s) 2160
BspHI TCATGA 2 cut(s) 208, 2083
BspLI GGNNCC 6 cut(s) 60, 119, 1366, 1573, 1646, 1775
BspMAI CTGCAG 5 cut(s) 108, 301, 736, 854, 1783
BspMI ACCTGC 2 cut(s) 2073, 2179
BspOI GCTAGC 1 cut(s) 2022
BspPI GGATC 1 cut(s) 1868
BspQI GCTCTTC 1 cut(s) 1752
BspT107I GGYRCC 1 cut(s) 1364
BspTNI GGTCTC 1 cut(s) 1376
BsrDI GCAATG 3 cut(s) 592, 1209, 2151
BsrFI RCCGGY 3 cut(s) 737, 1367, 2174
BsrGI TGTACA 1 cut(s) 264
BsrI ACTGG 5 cut(s) 493, 847, 1682, 2135, 2306
BssAI RCCGGY 3 cut(s) 737, 1367, 2174
BssECI CCNNGG 9 cut(s) 68, 1064, 1122, 1123, 1413, 1713, 1924, 1925, 1977
BssMI GATC 3 cut(s) 1313, 1822, 1873
BssNAI GTATAC 1 cut(s) 1161
BssT1I CCWWGG 3 cut(s) 1064, 1713, 1977
Bst1107I GTATAC 1 cut(s) 1161
Bst2UI CCWGG 3 cut(s) 69, 1124, 1620
Bst6I CTCTTC 1 cut(s) 1752
BstAPI GCANNNNNTGC 1 cut(s) 2028
BstAUI TGTACA 1 cut(s) 264
BstC8I GCNNGC 6 cut(s) 619, 760, 1087, 2020, 2158, 2176
BstDEI CTNAG 8 cut(s) 186, 198, 856, 1003, 1383, 1848, 1860, 1951
BstDSI CCRYGG 2 cut(s) 1713, 1977
BstENI CCTNNNNNAGG 1 cut(s) 66
BstF5I GGATG 9 cut(s) 725, 964, 1173, 1177, 1325, 1405, 1758, 1959, 2106
BstFNI CGCG 1 cut(s) 2160
BstH2I RGCGCY 1 cut(s) 1857
BstHHI GCGC 2 cut(s) 1856, 2006
BstKTI GATC 3 cut(s) 1316, 1825, 1876
BstMAI GTCTC 5 cut(s) 207, 510, 528, 1376, 1588
BstMBI GATC 3 cut(s) 1313, 1822, 1873
BstMWI GCNNNNNNNGC 4 cut(s) 1364, 1853, 2003, 2028
BstNI CCWGG 3 cut(s) 69, 1124, 1620
BstNSI RCATGY 2 cut(s) 1901, 2031
BstPAI GACNNNNGTC 1 cut(s) 2045
BstSCI CCNGG 7 cut(s) 67, 340, 1122, 1241, 1618, 1924, 1925
BstSFI CTRYAG 6 cut(s) 104, 297, 732, 850, 1140, 1779
BstSLI GKGCMC 1 cut(s) 619
BstUI CGCG 1 cut(s) 2160
BstV1I GCAGC 9 cut(s) 283, 746, 836, 859, 864, 1094, 1321, 1600, 1793
BstV2I GAAGAC 2 cut(s) 3, 1239
BstXI CCANNNNNNTGG 2 cut(s) 549, 810
BstZ17I GTATAC 1 cut(s) 1161
BsuI GTATCC 1 cut(s) 2322
BsuRI GGCC 4 cut(s) 24, 499, 992, 1032
BtgI CCRYGG 2 cut(s) 1713, 1977
BtrI CACGTC 1 cut(s) 907
BtsCI GGATG 9 cut(s) 725, 964, 1173, 1177, 1325, 1405, 1758, 1959, 2106
BtsI GCAGTG 2 cut(s) 729, 1329
BtsIMutI CAGTG 6 cut(s) 486, 729, 1074, 1243, 1329, 2346
BveI ACCTGC 2 cut(s) 2073, 2179
Cac8I GCNNGC 6 cut(s) 619, 760, 1087, 2020, 2158, 2176
CciI TCATGA 2 cut(s) 208, 2083
CfoI GCGC 2 cut(s) 1856, 2006
Cfr10I RCCGGY 3 cut(s) 737, 1367, 2174
Cfr13I GGNCC 5 cut(s) 498, 1031, 1571, 1644, 2132
Cfr9I CCCGGG 1 cut(s) 1925
Csp6I GTAC 6 cut(s) 265, 338, 714, 1272, 1595, 2299
CviQI GTAC 6 cut(s) 265, 338, 714, 1272, 1595, 2299
DdeI CTNAG 8 cut(s) 186, 198, 856, 1003, 1383, 1848, 1860, 1951
DpnI GATC 3 cut(s) 1315, 1824, 1875
DpnII GATC 3 cut(s) 1313, 1822, 1873
DraI TTTAAA 1 cut(s) 1693
EaeI YGGCCR 1 cut(s) 990
Eam1104I CTCTTC 1 cut(s) 1752
EarI CTCTTC 1 cut(s) 1752
EciI GGCGGA 1 cut(s) 921
Ecl136II GAGCTC 1 cut(s) 2089
Eco130I CCWWGG 3 cut(s) 1064, 1713, 1977
Eco24I GRGCYC 3 cut(s) 1778, 1932, 2091
Eco31I GGTCTC 1 cut(s) 1376
Eco32I GATATC 1 cut(s) 1539
Eco47I GGWCC 3 cut(s) 1571, 1644, 2132
Eco53kI GAGCTC 1 cut(s) 2089
Eco57I CTGAAG 4 cut(s) 50, 687, 762, 1619
Eco88I CYCGRG 4 cut(s) 500, 693, 1566, 1925
EcoICRI GAGCTC 1 cut(s) 2089
EcoNI CCTNNNNNAGG 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 1571
EcoRII CCWGG 3 cut(s) 67, 1122, 1618
EcoRV GATATC 1 cut(s) 1539
EcoT14I CCWWGG 3 cut(s) 1064, 1713, 1977
EcoT38I GRGCYC 3 cut(s) 1778, 1932, 2091
ErhI CCWWGG 3 cut(s) 1064, 1713, 1977
FalI AAGNNNNNCTT 2 cut(s) 2269, 2301
FaqI GGGAC 3 cut(s) 783, 1630, 2241
FauNDI CATATG 3 cut(s) 54, 223, 560
FblI GTMKAC 3 cut(s) 414, 1160, 1423
FokI GGATG 9 cut(s) 712, 971, 1180, 1184, 1312, 1412, 1745, 1946, 2113
FriOI GRGCYC 3 cut(s) 1778, 1932, 2091
FspBI CTAG 3 cut(s) 390, 923, 2019
GlaI GCGC 2 cut(s) 1855, 2005
GsaI CCCAGC 2 cut(s) 1362, 1395
GsuI CTGGAG 3 cut(s) 51, 1665, 2255
HaeII RGCGCY 1 cut(s) 1857
HaeIII GGCC 4 cut(s) 24, 499, 992, 1032
HapII CCGG 6 cut(s) 342, 738, 1242, 1368, 1926, 2175
HhaI GCGC 2 cut(s) 1856, 2006
Hin6I GCGC 2 cut(s) 1854, 2004
HinP1I GCGC 2 cut(s) 1854, 2004
HindIII AAGCTT 2 cut(s) 511, 760
HinfI GANTC 9 cut(s) 200, 214, 254, 416, 448, 477, 503, 999, 1700
HpaII CCGG 6 cut(s) 342, 738, 1242, 1368, 1926, 2175
HphI GGTGA 2 cut(s) 73, 2068
Hpy188I TCNGA 4 cut(s) 199, 1417, 1863, 1954
Hpy188III TCNNGA 9 cut(s) 209, 374, 566, 705, 780, 1311, 1704, 2084, 2112
HpyAV CCTTC 5 cut(s) 25, 131, 563, 1599, 2079
HpyCH4IV ACGT 1 cut(s) 906
HpyF10VI GCNNNNNNNGC 4 cut(s) 1364, 1853, 2003, 2028
HpyF3I CTNAG 8 cut(s) 186, 198, 856, 1003, 1383, 1848, 1860, 1951
HpySE526I ACGT 1 cut(s) 906
HspAI GCGC 2 cut(s) 1854, 2004
KroI GCCGGC 1 cut(s) 2174
KroNI GCCGGC 1 cut(s) 2176
Kzo9I GATC 3 cut(s) 1313, 1822, 1873
LguI GCTCTTC 1 cut(s) 1752
Lsp1109I GCAGC 9 cut(s) 283, 746, 836, 859, 864, 1094, 1321, 1600, 1793
LweI GCATC 7 cut(s) 688, 874, 926, 1213, 1223, 1767, 2031
MaeI CTAG 3 cut(s) 390, 923, 2019
MaeII ACGT 1 cut(s) 906
MaeIII GTNAC 2 cut(s) 79, 2119
MalI GATC 3 cut(s) 1315, 1824, 1875
MboI GATC 3 cut(s) 1313, 1822, 1873
MhlI GDGCHC 5 cut(s) 619, 802, 1778, 1932, 2091
MlsI TGGCCA 1 cut(s) 992
MluNI TGGCCA 1 cut(s) 992
MlyI GAGTC 5 cut(s) 209, 410, 457, 486, 512
MmeI TCCRAC 2 cut(s) 377, 530
Mox20I TGGCCA 1 cut(s) 992
MroNI GCCGGC 1 cut(s) 2174
MroXI GAANNNNTTC 3 cut(s) 258, 1181, 2281
MscI TGGCCA 1 cut(s) 992
MseI TTAA 8 cut(s) 630, 1505, 1692, 1752, 2055, 2139, 2187, 2193
MslI CAYNNNNRTG 1 cut(s) 53
Msp20I TGGCCA 1 cut(s) 992
MspI CCGG 6 cut(s) 342, 738, 1242, 1368, 1926, 2175
MspR9I CCNGG 7 cut(s) 69, 342, 1124, 1243, 1620, 1926, 1927
Mva1269I GAATGC 1 cut(s) 1553
MvaI CCWGG 3 cut(s) 69, 1124, 1620
MvnI CGCG 1 cut(s) 2160
MwoI GCNNNNNNNGC 4 cut(s) 1364, 1853, 2003, 2028
NaeI GCCGGC 1 cut(s) 2176
NciI CCSGG 4 cut(s) 342, 1243, 1926, 1927
NcoI CCATGG 2 cut(s) 1713, 1977
NdeI CATATG 3 cut(s) 54, 223, 560
NdeII GATC 3 cut(s) 1313, 1822, 1873
NgoMIV GCCGGC 1 cut(s) 2174
NheI GCTAGC 1 cut(s) 2018
NlaIV GGNNCC 6 cut(s) 60, 119, 1366, 1573, 1646, 1775
NmuCI GTSAC 2 cut(s) 79, 2119
NspI RCATGY 2 cut(s) 1901, 2031
PaeR7I CTCGAG 1 cut(s) 1566
PagI TCATGA 2 cut(s) 208, 2083
PaqCI CACCTGC 1 cut(s) 2179
PasI CCCWGGG 1 cut(s) 1123
PciI ACATGT 1 cut(s) 1897
PciSI GCTCTTC 1 cut(s) 1752
PctI GAATGC 1 cut(s) 1553
PdiI GCCGGC 1 cut(s) 2176
PdmI GAANNNNTTC 3 cut(s) 258, 1181, 2281
PfeI GAWTC 4 cut(s) 214, 254, 999, 1700
PleI GAGTC 5 cut(s) 208, 410, 456, 485, 511
PpsI GAGTC 5 cut(s) 208, 410, 456, 485, 511
PpuMI RGGWCCY 1 cut(s) 1571
PscI ACATGT 1 cut(s) 1897
PshAI GACNNNNGTC 1 cut(s) 2045
Psp124BI GAGCTC 1 cut(s) 2091
Psp5II RGGWCCY 1 cut(s) 1571
Psp6I CCWGG 3 cut(s) 67, 1122, 1618
PspFI CCCAGC 2 cut(s) 1358, 1391
PspGI CCWGG 3 cut(s) 67, 1122, 1618
PspN4I GGNNCC 6 cut(s) 60, 119, 1366, 1573, 1646, 1775
PspPI GGNCC 5 cut(s) 498, 1031, 1571, 1644, 2132
PspPPI RGGWCCY 1 cut(s) 1571
PspXI VCTCGAGB 1 cut(s) 1566
PsrI GAACNNNNNNTAC 2 cut(s) 1169, 1201
PstI CTGCAG 5 cut(s) 108, 301, 736, 854, 1783
RsaI GTAC 6 cut(s) 266, 339, 715, 1273, 1596, 2300
RsaNI GTAC 6 cut(s) 265, 338, 714, 1272, 1595, 2299
RseI CAYNNNNRTG 1 cut(s) 53
SacI GAGCTC 1 cut(s) 2091
SapI GCTCTTC 1 cut(s) 1752
SaqAI TTAA 8 cut(s) 630, 1505, 1692, 1752, 2055, 2139, 2187, 2193
Sau3AI GATC 3 cut(s) 1313, 1822, 1873
Sau96I GGNCC 5 cut(s) 498, 1031, 1571, 1644, 2132
SchI GAGTC 5 cut(s) 209, 410, 457, 486, 512
ScrFI CCNGG 7 cut(s) 69, 342, 1124, 1243, 1620, 1926, 1927
SduI GDGCHC 5 cut(s) 619, 802, 1778, 1932, 2091
SfaNI GCATC 7 cut(s) 688, 874, 926, 1213, 1223, 1767, 2031
SfcI CTRYAG 6 cut(s) 104, 297, 732, 850, 1140, 1779
Sfr274I CTCGAG 1 cut(s) 1566
SinI GGWCC 3 cut(s) 1571, 1644, 2132
SlaI CTCGAG 1 cut(s) 1566
SmaI CCCGGG 1 cut(s) 1927
SmiMI CAYNNNNRTG 1 cut(s) 53
SmlI CTYRAG 3 cut(s) 315, 1566, 2090
SmoI CTYRAG 3 cut(s) 315, 1566, 2090
SsiI CCGC 2 cut(s) 246, 932
SspMI CTAG 3 cut(s) 390, 923, 2019
SstI GAGCTC 1 cut(s) 2091
StyD4I CCNGG 7 cut(s) 67, 340, 1122, 1241, 1618, 1924, 1925
StyI CCWWGG 3 cut(s) 1064, 1713, 1977
TaiI ACGT 1 cut(s) 909
TaqI TCGA 6 cut(s) 1095, 1312, 1567, 1842, 1881, 2039
TatI WGTACW 2 cut(s) 264, 2298
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 4 cut(s) 214, 254, 999, 1700
Tru1I TTAA 8 cut(s) 630, 1505, 1692, 1752, 2055, 2139, 2187, 2193
Tru9I TTAA 8 cut(s) 630, 1505, 1692, 1752, 2055, 2139, 2187, 2193
TscAI CASTG 6 cut(s) 493, 736, 1081, 1243, 1336, 2346
TseFI GTSAC 2 cut(s) 79, 2119
TseI GCWGC 9 cut(s) 296, 734, 849, 852, 872, 1082, 1334, 1613, 1781
Tsp45I GTSAC 2 cut(s) 79, 2119
TspDTI ATGAA 4 cut(s) 654, 971, 2072, 2306
TspGWI ACGGA 2 cut(s) 1299, 1952
TspMI CCCGGG 1 cut(s) 1925
TspRI CASTG 6 cut(s) 493, 736, 1081, 1243, 1336, 2346
VneI GTGCAC 1 cut(s) 615
VpaK11BI GGWCC 3 cut(s) 1571, 1644, 2132
XagI CCTNNNNNAGG 1 cut(s) 66
XapI RAATTY 5 cut(s) 492, 647, 680, 1054, 1289
XceI RCATGY 2 cut(s) 1901, 2031
XhoI CTCGAG 1 cut(s) 1566
XmaI CCCGGG 1 cut(s) 1925
XmiI GTMKAC 3 cut(s) 414, 1160, 1423
XmnI GAANNNNTTC 3 cut(s) 258, 1181, 2281
XspI CTAG 3 cut(s) 390, 923, 2019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.