Rw6G013220

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0014653
Physical Location & Seq
Forward (+)
24383345 .. 24388795
5451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013220.1

Sequence Viewer

Length: 2325 bp
ATGGCTCCTCCTCCAGGGATTGCTGTCACCCTTTTGCTTCTTCTTGCTCTGCAGAGTTATGGGTTCCTTCTTTCTTTTGCTTCCACAAATGTTTACATTGTTTATATGGGAGAAAGAAAGTATGCAAATCCTGAGCTGGTATCTGAGTCTCATCATGAGATTCTGTCATATGTATTTGGAAGCAAAGAAGCCGCAAGGGAATCAATTCTGTACACCTACAAGCATGGATTTTCGGGGTTCGCTGCAGTTCTAACTCCATCTCAAGCCAAGCATATTGCAGATGTACCCGGTGTTGTTCGTGTCATTCCTCACAGGTTTCTTGATTTGCATACAACTAGAAGTTGGAACTTTCTGCAAGTAGACTCTCACTTTAGAAATGGAGTTCTTTCAAGGAGTCAATCAGGCGTTGGGTCTATTATTGGAGTCATGGACACTGGAATTTGGCCCGAGTCTCAAAGCTTTAGAGATGAGGGTATGGGAGACATTCCATCTCGTTGGAGAGGCATATGTCAAGAAGGAGAAAGGTTCAATCACTCCCATTGCAACAGGAAGATTATTGGTGCACGCTGGTATATTAAAGGATATGAAGCTGAATTTGGAAAGATAGATACAAGTGATGGGTTTGAATTTCTATCTCCCCGAGATGCTTCAGGACATGGTACTCACACATCATCCACTGCAGCCGGTGGTTTTATAGAAAATGCAAGCTTTATGGGACTTGCTTCAGGATTGGCAAGAGGAGGTGCTCCATCATCTTGGTTAGCTATTTACAAAGTTTGTTGGGCTACTGGTGGCTGCAGCTCAGCAGACATACTTGCTGCATTTGATGATGCAATTTTTGATGGTGTGGACGTGCTTTCGGCATCTCTAGGTTCTCCGCCTCCACTTGCTTCTTATGTTGAGGATGTTGTGTCCATTGGTTCTTTTCATGCTGTGGCCAAAGGAATCTCAGTTGTATGCTCTGCTGGAAACTCTGGCCCATACTCTCAAACTATCATAAATTCTGCTCCTTGGATATTCACTGTTGCAGCAAGCACTATCGACAGGGCTTTTCCTACTGCAATCTCCCTGGGAAACAATCAAACTGTAGTGGGTCAGGGTTTGTATACAGGGATGGATGTGAACAAGTTCTACCCTCTTGTGTATGGACAAGACATTGCATCTGTTGATGCAGACGAAGACAGTGCCGGGAGCTGTGGGTTGGGAACTCTAAATGGTACTTTAGCAAACGGAAAATTTGTTCTTTGTTTCCAATCTCGATCACAGAGGTCATCCACTGCTGCACAGAGAACTGTAATGAGTGCTGGGGGTGCCGGTCTCATTTTTGCTCAGTTTCCCAGCAAGGATGTTTCTTTGTCCTCGGATAGTCTACCGTGTGTTCAAATTGACTTTGCAATCGGCACATATATTCTCACATATATTGGAGCAACCAGAAATCCAATAGTCAAGTTAAACCCTACAAAAACAGTATTGGAACAGCAGATATCCCCAGAAGTGGCATTCTTCTCTGCTCGAGGACCCAATTCTGTCTCTCCTTCGGTACTGAAGCCCGATATTGCTGCTCCTGGTGTGAATATATTGTCCTCTTGGTCCCCTGCTTATTCACTATTACAACATCAACCATCTCCAGTCATCTTTAAAATTGAATCAGGAACTTCCATGGCTTGTCCACATATATCTGGTATAGTGGCTCTTCTTAAAAGCATCCACCCAATATGGAGCCCTGCAGCAATAAAGTCTGCCCTTATTACAACAGCCTCTTTAGAAGATCAATACGGTCAAAGTATCGAGGCTGAGGGCGCTCCTCAGAAGAAAGCTGATCCATTCGACTATGGTGGTGGACATGTCAATCCTAACAAAGCCATAGCCCCCGGGCTCATTTATGATATAGAAACCTCAGATTACATCCGTTTTCTTTGCTCCATGGGATATAACAATTCAGCCATCAGCGCAATCGCAGGAGCTAGCATCACATGCCATAAATCGACCAATGTCCAAGTTAATCTCAACCTGCCTTCCATCACCATTCATGAGCTCAAGGAAAGGATGACGGTATCAAGAACAGTCACAAATGTTGGTCCAGTTAATTCTATCTACATTGCTCGCGTTCAAACACCTGCCGGCGTGTCTATTAAAGTTAAGCCATCGGCTTTGTTGTTCAACTCTACTGCAAAGAAGCTGGAGTTCAAGGTTGTTTTTCGTCCCCTGCTAAAAGTTGCAGGAAGATTTTCTTTTGGAAATCTGTACTGGGAAGATGGTTTTCATGTAGTGAGGATACCTCTAACAGTGAGAACTGTCCTTGATGATTTTTACGCAGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

774

Amino Acids

82.8

Weight (kDa)

6.68

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 110 7e-18 Peptidase inhibitor I9
Peptidase_S8 PF00082 137 - 591 6e-44 Subtilase family
fn3_6 PF17766 668 - 763 9.8e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014653)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10210 FvH4_2g10210 FvH4_2g10210
malus_domestica MD05G1055900.v1.1
prunus_persica Prupe.8G086300_v2.0.a1
pyrus_communis pycom05g04760
rosa_chinensis RchiOBHm_Chr6g0267441
rosa_laevigata RLG00000014004
rosa_multiflora Rmu_sc0001068.1_g000015 Rmu_sc0006674.1_g000011
rosa_roxburghii Rroxscaffold_7G00200780
rosa_rugosa Rorug06G0032600 Rorug06G0032600
rosa_samantha Rh6AG154000 Rh6BG154100 Rh6CG149800
rosa_wichuraiana Rw6G013220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2125
Acc36I ACCTGC 2 cut(s) 2019, 2125
AccB1I GGYRCC 1 cut(s) 1310
AccI GTMKAC 3 cut(s) 360, 1106, 1369
AccII CGCG 1 cut(s) 2106
AciI CCGC 2 cut(s) 192, 878
AclWI GGATC 1 cut(s) 1814
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 5 cut(s) 438, 593, 626, 1000, 1235
AcuI CTGAAG 3 cut(s) 633, 708, 1565
AfaI GTAC 6 cut(s) 212, 285, 661, 1219, 1542, 2246
AfiI CCNNNNNNNGG 2 cut(s) 14, 1495
AflIII ACRYGT 1 cut(s) 1843
AgsI TTSAA 8 cut(s) 390, 529, 626, 1382, 1646, 2111, 2161, 2188
AjiI CACGTC 1 cut(s) 853
AjnI CCWGG 3 cut(s) 13, 1068, 1564
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AloI GAACNNNNNNTCC 2 cut(s) 1224, 1256
Alw21I GWGCWC 3 cut(s) 565, 748, 2037
Alw26I GTCTC 5 cut(s) 153, 456, 474, 1322, 1534
Alw44I GTGCAC 1 cut(s) 561
AlwI GGATC 1 cut(s) 1814
Ama87I CYCGRG 4 cut(s) 446, 639, 1512, 1871
AoxI GGCC 3 cut(s) 443, 936, 976
ApaLI GTGCAC 1 cut(s) 561
ApeKI GCWGC 9 cut(s) 242, 680, 795, 798, 818, 1028, 1280, 1559, 1727
ApoI RAATTY 5 cut(s) 438, 593, 626, 1000, 1235
Asp700I GAANNNNTTC 3 cut(s) 204, 1127, 2227
AspLEI GCGC 2 cut(s) 1802, 1952
AspS9I GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
AsuC2I CCSGG 4 cut(s) 288, 1189, 1872, 1873
AsuHPI GGTGA 2 cut(s) 19, 2014
AsuNHI GCTAGC 1 cut(s) 1964
AvaI CYCGRG 4 cut(s) 446, 639, 1512, 1871
AvaII GGWCC 3 cut(s) 1517, 1590, 2078
BaeGI GKGCMC 1 cut(s) 565
BalI TGGCCA 1 cut(s) 938
BanI GGYRCC 1 cut(s) 1310
BanII GRGCYC 3 cut(s) 1724, 1878, 2037
BbsI GAAGAC 1 cut(s) 1185
Bbv12I GWGCWC 3 cut(s) 565, 748, 2037
BbvCI CCTCAGC 1 cut(s) 1794
BbvI GCAGC 9 cut(s) 229, 692, 782, 805, 810, 1040, 1267, 1546, 1739
BcgI CGANNNNNNTGC 4 cut(s) 1166, 1200, 1541, 1575
BciT130I CCWGG 3 cut(s) 15, 1070, 1566
BciVI GTATCC 1 cut(s) 2268
BcnI CCSGG 4 cut(s) 288, 1189, 1872, 1873
BcoDI GTCTC 5 cut(s) 153, 456, 474, 1322, 1534
BfaI CTAG 3 cut(s) 336, 869, 1965
BfmI CTRYAG 6 cut(s) 50, 243, 678, 796, 1086, 1725
BfoI RGCGCY 1 cut(s) 1803
BfuAI ACCTGC 2 cut(s) 2019, 2125
BfuI GTATCC 1 cut(s) 2268
BlpI GCTNAGC 1 cut(s) 802
Bme1390I CCNGG 7 cut(s) 15, 288, 1070, 1189, 1566, 1872, 1873
Bme18I GGWCC 3 cut(s) 1517, 1590, 2078
BmeT110I CYCGRG 4 cut(s) 446, 639, 1512, 1871
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
BmiI GGNNCC 6 cut(s) 6, 65, 1312, 1519, 1592, 1721
BmrFI CCNGG 7 cut(s) 15, 288, 1070, 1189, 1566, 1872, 1873
BmrI ACTGGG 1 cut(s) 2257
BmsI GCATC 8 cut(s) 634, 820, 872, 1159, 1169, 1713, 1977, 2308
BmtI GCTAGC 1 cut(s) 1968
BmuI ACTGGG 1 cut(s) 2257
BoxI GACNNNNGTC 1 cut(s) 1991
BpiI GAAGAC 1 cut(s) 1185
BplI GAGNNNNNCTC 2 cut(s) 2263, 2295
BpmI CTGGAG 2 cut(s) 1611, 2201
Bpu10I CCTNAGC 2 cut(s) 132, 1794
Bpu1102I GCTNAGC 1 cut(s) 802
BpuEI CTTGAG 2 cut(s) 246, 2021
BpuMI CCSGG 4 cut(s) 288, 1189, 1872, 1873
BsaI GGTCTC 1 cut(s) 1322
BsaJI CCNNGG 9 cut(s) 14, 1010, 1068, 1069, 1359, 1659, 1870, 1871, 1923
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 1495
Bse118I RCCGGY 3 cut(s) 683, 1313, 2120
Bse1I ACTGG 5 cut(s) 439, 793, 1628, 2081, 2252
Bse3DI GCAATG 3 cut(s) 538, 1155, 2097
BseBI CCWGG 3 cut(s) 15, 1070, 1566
BseDI CCNNGG 9 cut(s) 14, 1010, 1068, 1069, 1359, 1659, 1870, 1871, 1923
BseGI GGATG 9 cut(s) 671, 910, 1119, 1123, 1271, 1351, 1704, 1905, 2052
BseLI CCNNNNNNNGG 2 cut(s) 14, 1495
BseMI GCAATG 3 cut(s) 538, 1155, 2097
BseMII CTCAG 8 cut(s) 123, 135, 816, 963, 1343, 1785, 1820, 1911
BseNI ACTGG 5 cut(s) 439, 793, 1628, 2081, 2252
BseRI GAGGAG 2 cut(s) 753, 1794
BseSI GKGCMC 1 cut(s) 565
BseXI GCAGC 9 cut(s) 229, 692, 782, 805, 810, 1040, 1267, 1546, 1739
BseYI CCCAGC 2 cut(s) 1304, 1337
BsgI GTGCAG 1 cut(s) 1266
Bsh1236I CGCG 1 cut(s) 2106
BshFI GGCC 3 cut(s) 445, 938, 978
BshNI GGYRCC 1 cut(s) 1310
BsiHKAI GWGCWC 3 cut(s) 565, 748, 2037
BsiHKCI CYCGRG 4 cut(s) 446, 639, 1512, 1871
BsiSI CCGG 6 cut(s) 288, 684, 1188, 1314, 1872, 2121
BslFI GGGAC 3 cut(s) 729, 1576, 2187
BslI CCNNNNNNNGG 2 cut(s) 14, 1495
BsmAI GTCTC 5 cut(s) 153, 456, 474, 1322, 1534
BsmFI GGGAC 3 cut(s) 729, 1576, 2187
BsmI GAATGC 1 cut(s) 1499
BsnI GGCC 3 cut(s) 445, 938, 978
Bso31I GGTCTC 1 cut(s) 1322
BsoBI CYCGRG 4 cut(s) 446, 639, 1512, 1871
Bsp1286I GDGCHC 5 cut(s) 565, 748, 1724, 1878, 2037
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 3 cut(s) 1259, 1768, 1819
Bsp1720I GCTNAGC 1 cut(s) 802
Bsp19I CCATGG 2 cut(s) 1659, 1923
BspACI CCGC 2 cut(s) 192, 878
BspANI GGCC 3 cut(s) 445, 938, 978
BspCNI CTCAG 8 cut(s) 124, 136, 815, 962, 1342, 1786, 1819, 1910
BspFNI CGCG 1 cut(s) 2106
BspHI TCATGA 2 cut(s) 154, 2029
BspLI GGNNCC 6 cut(s) 6, 65, 1312, 1519, 1592, 1721
BspMAI CTGCAG 5 cut(s) 54, 247, 682, 800, 1729
BspMI ACCTGC 2 cut(s) 2019, 2125
BspOI GCTAGC 1 cut(s) 1968
BspPI GGATC 1 cut(s) 1814
BspQI GCTCTTC 1 cut(s) 1698
BspT107I GGYRCC 1 cut(s) 1310
BspTNI GGTCTC 1 cut(s) 1322
BsrDI GCAATG 3 cut(s) 538, 1155, 2097
BsrFI RCCGGY 3 cut(s) 683, 1313, 2120
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 5 cut(s) 439, 793, 1628, 2081, 2252
BssAI RCCGGY 3 cut(s) 683, 1313, 2120
BssECI CCNNGG 9 cut(s) 14, 1010, 1068, 1069, 1359, 1659, 1870, 1871, 1923
BssMI GATC 3 cut(s) 1259, 1768, 1819
BssNAI GTATAC 1 cut(s) 1107
BssT1I CCWWGG 3 cut(s) 1010, 1659, 1923
Bst1107I GTATAC 1 cut(s) 1107
Bst2UI CCWGG 3 cut(s) 15, 1070, 1566
Bst6I CTCTTC 1 cut(s) 1698
BstAPI GCANNNNNTGC 1 cut(s) 1974
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 6 cut(s) 565, 706, 1033, 1966, 2104, 2122
BstDEI CTNAG 8 cut(s) 132, 144, 802, 949, 1329, 1794, 1806, 1897
BstDSI CCRYGG 2 cut(s) 1659, 1923
BstENI CCTNNNNNAGG 1 cut(s) 12
BstF5I GGATG 9 cut(s) 671, 910, 1119, 1123, 1271, 1351, 1704, 1905, 2052
BstFNI CGCG 1 cut(s) 2106
BstH2I RGCGCY 1 cut(s) 1803
BstHHI GCGC 2 cut(s) 1802, 1952
BstKTI GATC 3 cut(s) 1262, 1771, 1822
BstMAI GTCTC 5 cut(s) 153, 456, 474, 1322, 1534
BstMBI GATC 3 cut(s) 1259, 1768, 1819
BstMWI GCNNNNNNNGC 4 cut(s) 1310, 1799, 1949, 1974
BstNI CCWGG 3 cut(s) 15, 1070, 1566
BstNSI RCATGY 2 cut(s) 1847, 1977
BstPAI GACNNNNGTC 1 cut(s) 1991
BstSCI CCNGG 7 cut(s) 13, 286, 1068, 1187, 1564, 1870, 1871
BstSFI CTRYAG 6 cut(s) 50, 243, 678, 796, 1086, 1725
BstSLI GKGCMC 1 cut(s) 565
BstUI CGCG 1 cut(s) 2106
BstV1I GCAGC 9 cut(s) 229, 692, 782, 805, 810, 1040, 1267, 1546, 1739
BstV2I GAAGAC 1 cut(s) 1185
BstXI CCANNNNNNTGG 2 cut(s) 495, 756
BstZ17I GTATAC 1 cut(s) 1107
BsuI GTATCC 1 cut(s) 2268
BsuRI GGCC 3 cut(s) 445, 938, 978
BtgI CCRYGG 2 cut(s) 1659, 1923
BtrI CACGTC 1 cut(s) 853
BtsCI GGATG 9 cut(s) 671, 910, 1119, 1123, 1271, 1351, 1704, 1905, 2052
BtsI GCAGTG 2 cut(s) 675, 1275
BtsIMutI CAGTG 6 cut(s) 432, 675, 1020, 1189, 1275, 2292
BveI ACCTGC 2 cut(s) 2019, 2125
Cac8I GCNNGC 6 cut(s) 565, 706, 1033, 1966, 2104, 2122
CciI TCATGA 2 cut(s) 154, 2029
CfoI GCGC 2 cut(s) 1802, 1952
Cfr10I RCCGGY 3 cut(s) 683, 1313, 2120
Cfr13I GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
Cfr9I CCCGGG 1 cut(s) 1871
Csp6I GTAC 6 cut(s) 211, 284, 660, 1218, 1541, 2245
CviQI GTAC 6 cut(s) 211, 284, 660, 1218, 1541, 2245
DdeI CTNAG 8 cut(s) 132, 144, 802, 949, 1329, 1794, 1806, 1897
DpnI GATC 3 cut(s) 1261, 1770, 1821
DpnII GATC 3 cut(s) 1259, 1768, 1819
DraI TTTAAA 1 cut(s) 1639
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 1 cut(s) 1698
EarI CTCTTC 1 cut(s) 1698
EciI GGCGGA 1 cut(s) 867
Ecl136II GAGCTC 1 cut(s) 2035
Eco130I CCWWGG 3 cut(s) 1010, 1659, 1923
Eco24I GRGCYC 3 cut(s) 1724, 1878, 2037
Eco31I GGTCTC 1 cut(s) 1322
Eco32I GATATC 1 cut(s) 1485
Eco47I GGWCC 3 cut(s) 1517, 1590, 2078
Eco53kI GAGCTC 1 cut(s) 2035
Eco57I CTGAAG 3 cut(s) 633, 708, 1565
Eco88I CYCGRG 4 cut(s) 446, 639, 1512, 1871
EcoICRI GAGCTC 1 cut(s) 2035
EcoNI CCTNNNNNAGG 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 1517
EcoRII CCWGG 3 cut(s) 13, 1068, 1564
EcoRV GATATC 1 cut(s) 1485
EcoT14I CCWWGG 3 cut(s) 1010, 1659, 1923
EcoT22I ATGCAT 1 cut(s) 2323
EcoT38I GRGCYC 3 cut(s) 1724, 1878, 2037
ErhI CCWWGG 3 cut(s) 1010, 1659, 1923
FalI AAGNNNNNCTT 2 cut(s) 2215, 2247
FaqI GGGAC 3 cut(s) 729, 1576, 2187
FauNDI CATATG 2 cut(s) 169, 506
FblI GTMKAC 3 cut(s) 360, 1106, 1369
FokI GGATG 9 cut(s) 658, 917, 1126, 1130, 1258, 1358, 1691, 1892, 2059
FriOI GRGCYC 3 cut(s) 1724, 1878, 2037
FspBI CTAG 3 cut(s) 336, 869, 1965
GlaI GCGC 2 cut(s) 1801, 1951
GsaI CCCAGC 2 cut(s) 1308, 1341
GsuI CTGGAG 2 cut(s) 1611, 2201
HaeII RGCGCY 1 cut(s) 1803
HaeIII GGCC 3 cut(s) 445, 938, 978
HapII CCGG 6 cut(s) 288, 684, 1188, 1314, 1872, 2121
HhaI GCGC 2 cut(s) 1802, 1952
Hin6I GCGC 2 cut(s) 1800, 1950
HinP1I GCGC 2 cut(s) 1800, 1950
HindIII AAGCTT 2 cut(s) 457, 706
HinfI GANTC 9 cut(s) 146, 160, 200, 362, 394, 423, 449, 945, 1646
HpaII CCGG 6 cut(s) 288, 684, 1188, 1314, 1872, 2121
HphI GGTGA 2 cut(s) 19, 2014
Hpy188I TCNGA 4 cut(s) 145, 1363, 1809, 1900
HpyAV CCTTC 4 cut(s) 77, 509, 1545, 2025
HpyCH4IV ACGT 1 cut(s) 852
HpyF10VI GCNNNNNNNGC 4 cut(s) 1310, 1799, 1949, 1974
HpyF3I CTNAG 8 cut(s) 132, 144, 802, 949, 1329, 1794, 1806, 1897
HpySE526I ACGT 1 cut(s) 852
HspAI GCGC 2 cut(s) 1800, 1950
KroI GCCGGC 1 cut(s) 2120
KroNI GCCGGC 1 cut(s) 2122
Kzo9I GATC 3 cut(s) 1259, 1768, 1819
LguI GCTCTTC 1 cut(s) 1698
Lsp1109I GCAGC 9 cut(s) 229, 692, 782, 805, 810, 1040, 1267, 1546, 1739
LweI GCATC 8 cut(s) 634, 820, 872, 1159, 1169, 1713, 1977, 2308
MaeI CTAG 3 cut(s) 336, 869, 1965
MaeII ACGT 1 cut(s) 852
MaeIII GTNAC 2 cut(s) 25, 2065
MalI GATC 3 cut(s) 1261, 1770, 1821
MboI GATC 3 cut(s) 1259, 1768, 1819
MboII GAAGA 9 cut(s) 32, 562, 1190, 1495, 1685, 1778, 1822, 2235, 2264
MhlI GDGCHC 5 cut(s) 565, 748, 1724, 1878, 2037
MlsI TGGCCA 1 cut(s) 938
MluNI TGGCCA 1 cut(s) 938
MlyI GAGTC 5 cut(s) 155, 356, 403, 432, 458
MmeI TCCRAC 2 cut(s) 323, 476
Mox20I TGGCCA 1 cut(s) 938
Mph1103I ATGCAT 1 cut(s) 2323
MroNI GCCGGC 1 cut(s) 2120
MroXI GAANNNNTTC 3 cut(s) 204, 1127, 2227
MscI TGGCCA 1 cut(s) 938
MseI TTAA 8 cut(s) 576, 1451, 1638, 1698, 2001, 2085, 2133, 2139
Msp20I TGGCCA 1 cut(s) 938
MspI CCGG 6 cut(s) 288, 684, 1188, 1314, 1872, 2121
MspR9I CCNGG 7 cut(s) 15, 288, 1070, 1189, 1566, 1872, 1873
Mva1269I GAATGC 1 cut(s) 1499
MvaI CCWGG 3 cut(s) 15, 1070, 1566
MvnI CGCG 1 cut(s) 2106
MwoI GCNNNNNNNGC 4 cut(s) 1310, 1799, 1949, 1974
NaeI GCCGGC 1 cut(s) 2122
NciI CCSGG 4 cut(s) 288, 1189, 1872, 1873
NcoI CCATGG 2 cut(s) 1659, 1923
NdeI CATATG 2 cut(s) 169, 506
NdeII GATC 3 cut(s) 1259, 1768, 1819
NgoMIV GCCGGC 1 cut(s) 2120
NheI GCTAGC 1 cut(s) 1964
NlaIV GGNNCC 6 cut(s) 6, 65, 1312, 1519, 1592, 1721
NmuCI GTSAC 2 cut(s) 25, 2065
NsiI ATGCAT 1 cut(s) 2323
NspI RCATGY 2 cut(s) 1847, 1977
PaeR7I CTCGAG 1 cut(s) 1512
PagI TCATGA 2 cut(s) 154, 2029
PaqCI CACCTGC 1 cut(s) 2125
PasI CCCWGGG 1 cut(s) 1069
PciI ACATGT 1 cut(s) 1843
PciSI GCTCTTC 1 cut(s) 1698
PctI GAATGC 1 cut(s) 1499
PdiI GCCGGC 1 cut(s) 2122
PdmI GAANNNNTTC 3 cut(s) 204, 1127, 2227
PfeI GAWTC 4 cut(s) 160, 200, 945, 1646
PleI GAGTC 5 cut(s) 154, 356, 402, 431, 457
PpsI GAGTC 5 cut(s) 154, 356, 402, 431, 457
PpuMI RGGWCCY 1 cut(s) 1517
PscI ACATGT 1 cut(s) 1843
PshAI GACNNNNGTC 1 cut(s) 1991
Psp124BI GAGCTC 1 cut(s) 2037
Psp5II RGGWCCY 1 cut(s) 1517
Psp6I CCWGG 3 cut(s) 13, 1068, 1564
PspFI CCCAGC 2 cut(s) 1304, 1337
PspGI CCWGG 3 cut(s) 13, 1068, 1564
PspN4I GGNNCC 6 cut(s) 6, 65, 1312, 1519, 1592, 1721
PspPI GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
PspPPI RGGWCCY 1 cut(s) 1517
PspXI VCTCGAGB 1 cut(s) 1512
PsrI GAACNNNNNNTAC 2 cut(s) 1115, 1147
PstI CTGCAG 5 cut(s) 54, 247, 682, 800, 1729
RsaI GTAC 6 cut(s) 212, 285, 661, 1219, 1542, 2246
RsaNI GTAC 6 cut(s) 211, 284, 660, 1218, 1541, 2245
SacI GAGCTC 1 cut(s) 2037
SapI GCTCTTC 1 cut(s) 1698
SaqAI TTAA 8 cut(s) 576, 1451, 1638, 1698, 2001, 2085, 2133, 2139
Sau3AI GATC 3 cut(s) 1259, 1768, 1819
Sau96I GGNCC 5 cut(s) 444, 977, 1517, 1590, 2078
SchI GAGTC 5 cut(s) 155, 356, 403, 432, 458
ScrFI CCNGG 7 cut(s) 15, 288, 1070, 1189, 1566, 1872, 1873
SduI GDGCHC 5 cut(s) 565, 748, 1724, 1878, 2037
SfaNI GCATC 8 cut(s) 634, 820, 872, 1159, 1169, 1713, 1977, 2308
SfcI CTRYAG 6 cut(s) 50, 243, 678, 796, 1086, 1725
Sfr274I CTCGAG 1 cut(s) 1512
SinI GGWCC 3 cut(s) 1517, 1590, 2078
SlaI CTCGAG 1 cut(s) 1512
SmaI CCCGGG 1 cut(s) 1873
SmlI CTYRAG 3 cut(s) 261, 1512, 2036
SmoI CTYRAG 3 cut(s) 261, 1512, 2036
SsiI CCGC 2 cut(s) 192, 878
SspMI CTAG 3 cut(s) 336, 869, 1965
SstI GAGCTC 1 cut(s) 2037
StyD4I CCNGG 7 cut(s) 13, 286, 1068, 1187, 1564, 1870, 1871
StyI CCWWGG 3 cut(s) 1010, 1659, 1923
TaiI ACGT 1 cut(s) 855
TaqI TCGA 6 cut(s) 1041, 1258, 1513, 1788, 1827, 1985
TatI WGTACW 2 cut(s) 210, 2244
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 4 cut(s) 160, 200, 945, 1646
Tru1I TTAA 8 cut(s) 576, 1451, 1638, 1698, 2001, 2085, 2133, 2139
Tru9I TTAA 8 cut(s) 576, 1451, 1638, 1698, 2001, 2085, 2133, 2139
TscAI CASTG 6 cut(s) 439, 682, 1027, 1189, 1282, 2292
TseFI GTSAC 2 cut(s) 25, 2065
TseI GCWGC 9 cut(s) 242, 680, 795, 798, 818, 1028, 1280, 1559, 1727
Tsp45I GTSAC 2 cut(s) 25, 2065
TspDTI ATGAA 4 cut(s) 600, 917, 2018, 2252
TspGWI ACGGA 2 cut(s) 1245, 1898
TspMI CCCGGG 1 cut(s) 1871
TspRI CASTG 6 cut(s) 439, 682, 1027, 1189, 1282, 2292
VneI GTGCAC 1 cut(s) 561
VpaK11BI GGWCC 3 cut(s) 1517, 1590, 2078
XagI CCTNNNNNAGG 1 cut(s) 12
XapI RAATTY 5 cut(s) 438, 593, 626, 1000, 1235
XceI RCATGY 2 cut(s) 1847, 1977
XhoI CTCGAG 1 cut(s) 1512
XmaI CCCGGG 1 cut(s) 1871
XmiI GTMKAC 3 cut(s) 360, 1106, 1369
XmnI GAANNNNTTC 3 cut(s) 204, 1127, 2227
XspI CTAG 3 cut(s) 336, 869, 1965
Zsp2I ATGCAT 1 cut(s) 2323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.