Subtilase family
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_2g10210 FvH4_2g10210 FvH4_2g10210 |
| malus_domestica | MD05G1055900.v1.1 |
| prunus_persica | Prupe.8G086300_v2.0.a1 |
| pyrus_communis | pycom05g04760 |
| rosa_chinensis | RchiOBHm_Chr6g0267441 |
| rosa_laevigata | RLG00000014004 |
| rosa_multiflora | Rmu_sc0001068.1_g000015 Rmu_sc0006674.1_g000011 |
| rosa_roxburghii | Rroxscaffold_7G00200780 |
| rosa_rugosa | Rorug06G0032600 Rorug06G0032600 |
| rosa_samantha | Rh6AG154000 Rh6BG154100 Rh6CG149800 |
| rosa_wichuraiana | Rw6G013220 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 525 |
| Acc36I | ACCTGC | 1 cut(s) | 2049 |
| AccI | GTMKAC | 3 cut(s) | 807, 1145, 2124 |
| AciI | CCGC | 2 cut(s) | 764, 1869 |
| AclWI | GGATC | 3 cut(s) | 1844, 1874, 1887 |
| AcsI | RAATTY | 8 cut(s) | 385, 420, 477, 632, 665, 1009, 1039, 2021 |
| AcuI | CTGAAG | 3 cut(s) | 484, 708, 2087 |
| AcyI | GRCGYC | 1 cut(s) | 522 |
| AfaI | GTAC | 3 cut(s) | 649, 700, 826 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 115, 1404 |
| AjnI | CCWGG | 8 cut(s) | 272, 325, 1049, 1107, 1603, 1625, 1773, 1900 |
| Alw21I | GWGCWC | 3 cut(s) | 604, 787, 1834 |
| Alw26I | GTCTC | 5 cut(s) | 290, 513, 678, 1361, 1910 |
| Alw44I | GTGCAC | 1 cut(s) | 600 |
| AlwI | GGATC | 3 cut(s) | 1844, 1874, 1887 |
| Ama87I | CYCGRG | 4 cut(s) | 485, 678, 1399, 1551 |
| AoxI | GGCC | 2 cut(s) | 117, 482 |
| ApaLI | GTGCAC | 1 cut(s) | 600 |
| ApeKI | GCWGC | 8 cut(s) | 719, 761, 831, 834, 837, 857, 1067, 1757 |
| ApoI | RAATTY | 8 cut(s) | 385, 420, 477, 632, 665, 1009, 1039, 2021 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 424, 1166 |
| AspLEI | GCGC | 1 cut(s) | 446 |
| AspS9I | GGNCC | 6 cut(s) | 201, 483, 1016, 1556, 1629, 2108 |
| AsuC2I | CCSGG | 2 cut(s) | 73, 1228 |
| AsuHPI | GGTGA | 4 cut(s) | 118, 280, 1202, 2044 |
| AsuII | TTCGAA | 1 cut(s) | 2320 |
| AvaI | CYCGRG | 4 cut(s) | 485, 678, 1399, 1551 |
| AvaII | GGWCC | 5 cut(s) | 201, 1016, 1556, 1629, 2108 |
| BaeGI | GKGCMC | 2 cut(s) | 50, 604 |
| BamHI | GGATCC | 1 cut(s) | 1879 |
| BanII | GRGCYC | 1 cut(s) | 1834 |
| BauI | CACGAG | 1 cut(s) | 2133 |
| BbsI | GAAGAC | 1 cut(s) | 1224 |
| Bbv12I | GWGCWC | 3 cut(s) | 604, 787, 1834 |
| BbvCI | CCTCAGC | 1 cut(s) | 1824 |
| BbvI | GCAGC | 8 cut(s) | 731, 748, 821, 843, 844, 849, 1079, 1744 |
| BciT130I | CCWGG | 8 cut(s) | 274, 327, 1051, 1109, 1605, 1627, 1775, 1902 |
| BciVI | GTATCC | 1 cut(s) | 1150 |
| BcnI | CCSGG | 2 cut(s) | 73, 1228 |
| BcoDI | GTCTC | 5 cut(s) | 290, 513, 678, 1361, 1910 |
| BfaI | CTAG | 7 cut(s) | 375, 774, 795, 854, 1377, 1481, 1793 |
| BfmI | CTRYAG | 3 cut(s) | 717, 835, 972 |
| BfoI | RGCGCY | 1 cut(s) | 447 |
| BfuAI | ACCTGC | 1 cut(s) | 2049 |
| BfuI | GTATCC | 1 cut(s) | 1150 |
| BisI | GCNGC | 8 cut(s) | 720, 762, 832, 835, 838, 858, 1068, 1758 |
| BlpI | GCTNAGC | 1 cut(s) | 841 |
| BlsI | GCNGC | 8 cut(s) | 721, 763, 833, 836, 839, 859, 1069, 1759 |
| Bme18I | GGWCC | 5 cut(s) | 201, 1016, 1556, 1629, 2108 |
| BmeT110I | CYCGRG | 4 cut(s) | 485, 678, 1399, 1551 |
| BmgT120I | GGNCC | 6 cut(s) | 201, 483, 1016, 1556, 1629, 2108 |
| BmiI | GGNNCC | 5 cut(s) | 202, 1558, 1631, 1684, 1881 |
| BmsI | GCATC | 5 cut(s) | 673, 719, 859, 911, 1198 |
| BpiI | GAAGAC | 1 cut(s) | 1224 |
| BpmI | CTGGAG | 3 cut(s) | 1512, 1641, 2231 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1824 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 841 |
| Bpu14I | TTCGAA | 1 cut(s) | 2320 |
| BpuMI | CCSGG | 2 cut(s) | 73, 1228 |
| BsaHI | GRCGYC | 1 cut(s) | 522 |
| BsaI | GGTCTC | 3 cut(s) | 678, 1361, 1910 |
| BsaWI | WCCGGW | 1 cut(s) | 2110 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 115, 1404 |
| Bse118I | RCCGGY | 1 cut(s) | 2150 |
| Bse1I | ACTGG | 3 cut(s) | 478, 832, 2316 |
| Bse3DI | GCAATG | 2 cut(s) | 421, 1194 |
| BseBI | CCWGG | 8 cut(s) | 274, 327, 1051, 1109, 1605, 1627, 1775, 1902 |
| BseGI | GGATG | 9 cut(s) | 36, 342, 949, 1158, 1390, 1646, 1738, 1804, 1935 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 115, 1404 |
| BseMI | GCAATG | 2 cut(s) | 421, 1194 |
| BseMII | CTCAG | 5 cut(s) | 272, 855, 1254, 1382, 1815 |
| BseNI | ACTGG | 3 cut(s) | 478, 832, 2316 |
| BseRI | GAGGAG | 3 cut(s) | 106, 792, 1824 |
| BseSI | GKGCMC | 2 cut(s) | 50, 604 |
| BseXI | GCAGC | 8 cut(s) | 731, 748, 821, 843, 844, 849, 1079, 1744 |
| BseYI | CCCAGC | 2 cut(s) | 446, 1988 |
| BshFI | GGCC | 2 cut(s) | 119, 484 |
| BsiHKAI | GWGCWC | 3 cut(s) | 604, 787, 1834 |
| BsiHKCI | CYCGRG | 4 cut(s) | 485, 678, 1399, 1551 |
| BsiSI | CCGG | 4 cut(s) | 73, 1227, 2111, 2151 |
| BslFI | GGGAC | 5 cut(s) | 509, 1527, 1615, 1683, 2005 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 115, 1404 |
| BsmAI | GTCTC | 5 cut(s) | 290, 513, 678, 1361, 1910 |
| BsmBI | CGTCTC | 1 cut(s) | 513 |
| BsmFI | GGGAC | 5 cut(s) | 509, 1527, 1615, 1683, 2005 |
| BsmI | GAATGC | 1 cut(s) | 1538 |
| BsnI | GGCC | 2 cut(s) | 119, 484 |
| Bso31I | GGTCTC | 3 cut(s) | 678, 1361, 1910 |
| BsoBI | CYCGRG | 4 cut(s) | 485, 678, 1399, 1551 |
| Bsp119I | TTCGAA | 1 cut(s) | 2320 |
| Bsp1286I | GDGCHC | 4 cut(s) | 50, 604, 787, 1834 |
| Bsp143I | GATC | 4 cut(s) | 1081, 1849, 1879, 2335 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 841 |
| Bsp19I | CCATGG | 1 cut(s) | 1953 |
| BspACI | CCGC | 2 cut(s) | 764, 1869 |
| BspANI | GGCC | 2 cut(s) | 119, 484 |
| BspCNI | CTCAG | 5 cut(s) | 273, 854, 1253, 1381, 1816 |
| BspHI | TCATGA | 1 cut(s) | 2059 |
| BspLI | GGNNCC | 5 cut(s) | 202, 1558, 1631, 1684, 1881 |
| BspMAI | CTGCAG | 2 cut(s) | 721, 839 |
| BspMI | ACCTGC | 1 cut(s) | 2049 |
| BspPI | GGATC | 3 cut(s) | 1844, 1874, 1887 |
| BspQI | GCTCTTC | 1 cut(s) | 257 |
| BspT104I | TTCGAA | 1 cut(s) | 2320 |
| BspTNI | GGTCTC | 3 cut(s) | 678, 1361, 1910 |
| BsrDI | GCAATG | 2 cut(s) | 421, 1194 |
| BsrFI | RCCGGY | 1 cut(s) | 2150 |
| BsrI | ACTGG | 3 cut(s) | 478, 832, 2316 |
| BssAI | RCCGGY | 1 cut(s) | 2150 |
| BssMI | GATC | 4 cut(s) | 1081, 1849, 1879, 2335 |
| BssNAI | GTATAC | 1 cut(s) | 1146 |
| BssNI | GRCGYC | 1 cut(s) | 522 |
| BssSI | CACGAG | 1 cut(s) | 2133 |
| BssT1I | CCWWGG | 1 cut(s) | 1953 |
| Bst1107I | GTATAC | 1 cut(s) | 1146 |
| Bst2BI | CACGAG | 1 cut(s) | 2133 |
| Bst2UI | CCWGG | 8 cut(s) | 274, 327, 1051, 1109, 1605, 1627, 1775, 1902 |
| Bst6I | CTCTTC | 3 cut(s) | 257, 1552, 2086 |
| BstACI | GRCGYC | 1 cut(s) | 522 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 716, 725 |
| BstBI | TTCGAA | 1 cut(s) | 2320 |
| BstC8I | GCNNGC | 3 cut(s) | 745, 1072, 1845 |
| BstDEI | CTNAG | 8 cut(s) | 281, 644, 841, 1240, 1368, 1824, 1981, 2240 |
| BstDSI | CCRYGG | 1 cut(s) | 1953 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1402 |
| BstF5I | GGATG | 9 cut(s) | 36, 342, 949, 1158, 1390, 1646, 1738, 1804, 1935 |
| BstH2I | RGCGCY | 1 cut(s) | 447 |
| BstHHI | GCGC | 1 cut(s) | 446 |
| BstKTI | GATC | 4 cut(s) | 1084, 1852, 1882, 2338 |
| BstMAI | GTCTC | 5 cut(s) | 290, 513, 678, 1361, 1910 |
| BstMBI | GATC | 4 cut(s) | 1081, 1849, 1879, 2335 |
| BstNI | CCWGG | 8 cut(s) | 274, 327, 1051, 1109, 1605, 1627, 1775, 1902 |
| BstNSI | RCATGY | 2 cut(s) | 2007, 2297 |
| BstSFI | CTRYAG | 3 cut(s) | 717, 835, 972 |
| BstSLI | GKGCMC | 2 cut(s) | 50, 604 |
| BstV1I | GCAGC | 8 cut(s) | 731, 748, 821, 843, 844, 849, 1079, 1744 |
| BstV2I | GAAGAC | 1 cut(s) | 1224 |
| BstX2I | RGATCY | 1 cut(s) | 1879 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 534, 1709 |
| BstYI | RGATCY | 1 cut(s) | 1879 |
| BstZ17I | GTATAC | 1 cut(s) | 1146 |
| BsuI | GTATCC | 1 cut(s) | 1150 |
| BsuRI | GGCC | 2 cut(s) | 119, 484 |
| BtgI | CCRYGG | 1 cut(s) | 1953 |
| BtsCI | GGATG | 9 cut(s) | 36, 342, 949, 1158, 1390, 1646, 1738, 1804, 1935 |
| BtsIMutI | CAGTG | 7 cut(s) | 50, 471, 577, 1059, 1228, 1338, 2309 |
| BveI | ACCTGC | 1 cut(s) | 2049 |
| Cac8I | GCNNGC | 3 cut(s) | 745, 1072, 1845 |
| CciI | TCATGA | 1 cut(s) | 2059 |
| CfoI | GCGC | 1 cut(s) | 446 |
| Cfr10I | RCCGGY | 1 cut(s) | 2150 |
| Cfr13I | GGNCC | 6 cut(s) | 201, 483, 1016, 1556, 1629, 2108 |
| CpoI | CGGWCCG | 1 cut(s) | 2108 |
| CsiI | ACCWGGT | 1 cut(s) | 272 |
| Csp6I | GTAC | 3 cut(s) | 648, 699, 825 |
| CspI | CGGWCCG | 1 cut(s) | 2108 |
| CviQI | GTAC | 3 cut(s) | 648, 699, 825 |
| DdeI | CTNAG | 8 cut(s) | 281, 644, 841, 1240, 1368, 1824, 1981, 2240 |
| DpnI | GATC | 4 cut(s) | 1083, 1851, 1881, 2337 |
| DpnII | GATC | 4 cut(s) | 1081, 1849, 1879, 2335 |
| Eam1104I | CTCTTC | 3 cut(s) | 257, 1552, 2086 |
| EarI | CTCTTC | 3 cut(s) | 257, 1552, 2086 |
| Ecl136II | GAGCTC | 1 cut(s) | 1832 |
| Eco130I | CCWWGG | 1 cut(s) | 1953 |
| Eco147I | AGGCCT | 1 cut(s) | 119 |
| Eco24I | GRGCYC | 1 cut(s) | 1834 |
| Eco31I | GGTCTC | 3 cut(s) | 678, 1361, 1910 |
| Eco32I | GATATC | 2 cut(s) | 322, 1524 |
| Eco47I | GGWCC | 5 cut(s) | 201, 1016, 1556, 1629, 2108 |
| Eco53kI | GAGCTC | 1 cut(s) | 1832 |
| Eco57I | CTGAAG | 3 cut(s) | 484, 708, 2087 |
| Eco88I | CYCGRG | 4 cut(s) | 485, 678, 1399, 1551 |
| EcoICRI | GAGCTC | 1 cut(s) | 1832 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1402 |
| EcoO109I | RGGNCCY | 2 cut(s) | 201, 1556 |
| EcoRI | GAATTC | 2 cut(s) | 420, 632 |
| EcoRII | CCWGG | 8 cut(s) | 272, 325, 1049, 1107, 1603, 1625, 1773, 1900 |
| EcoRV | GATATC | 2 cut(s) | 322, 1524 |
| EcoT14I | CCWWGG | 1 cut(s) | 1953 |
| EcoT38I | GRGCYC | 1 cut(s) | 1834 |
| ErhI | CCWWGG | 1 cut(s) | 1953 |
| Esp3I | CGTCTC | 1 cut(s) | 513 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2245, 2277 |
| FaqI | GGGAC | 5 cut(s) | 509, 1527, 1615, 1683, 2005 |
| FauI | CCCGC | 1 cut(s) | 757 |
| FblI | GTMKAC | 3 cut(s) | 807, 1145, 2124 |
| Fnu4HI | GCNGC | 8 cut(s) | 720, 762, 832, 835, 838, 858, 1068, 1758 |
| FokI | GGATG | 9 cut(s) | 23, 329, 956, 1165, 1397, 1633, 1725, 1811, 1922 |
| FriOI | GRGCYC | 1 cut(s) | 1834 |
| Fsp4HI | GCNGC | 8 cut(s) | 720, 762, 832, 835, 838, 858, 1068, 1758 |
| FspBI | CTAG | 7 cut(s) | 375, 774, 795, 854, 1377, 1481, 1793 |
| GlaI | GCGC | 1 cut(s) | 445 |
| GluI | GCNGC | 8 cut(s) | 720, 762, 832, 835, 838, 858, 1068, 1758 |
| GsaI | CCCAGC | 2 cut(s) | 450, 1992 |
| GsuI | CTGGAG | 3 cut(s) | 1512, 1641, 2231 |
| HaeII | RGCGCY | 1 cut(s) | 447 |
| HaeIII | GGCC | 2 cut(s) | 119, 484 |
| HapII | CCGG | 4 cut(s) | 73, 1227, 2111, 2151 |
| HhaI | GCGC | 1 cut(s) | 446 |
| Hin1I | GRCGYC | 1 cut(s) | 522 |
| Hin6I | GCGC | 1 cut(s) | 444 |
| HinP1I | GCGC | 1 cut(s) | 444 |
| HincII | GTYRAC | 1 cut(s) | 1426 |
| HindII | GTYRAC | 1 cut(s) | 1426 |
| HindIII | AAGCTT | 2 cut(s) | 496, 745 |
| HpaII | CCGG | 4 cut(s) | 73, 1227, 2111, 2151 |
| HphI | GGTGA | 4 cut(s) | 118, 280, 1202, 2044 |
| Hpy166II | GTNNAC | 7 cut(s) | 231, 400, 602, 808, 1146, 1426, 2125 |
| Hpy188I | TCNGA | 6 cut(s) | 282, 503, 638, 1328, 1930, 2267 |
| Hpy8I | GTNNAC | 7 cut(s) | 231, 400, 602, 808, 1146, 1426, 2125 |
| HpyAV | CCTTC | 8 cut(s) | 214, 548, 683, 939, 1333, 1584, 1675, 2055 |
| HpyCH4IV | ACGT | 1 cut(s) | 522 |
| HpyF3I | CTNAG | 8 cut(s) | 281, 644, 841, 1240, 1368, 1824, 1981, 2240 |
| HpySE526I | ACGT | 1 cut(s) | 522 |
| Hsp92I | GRCGYC | 1 cut(s) | 522 |
| HspAI | GCGC | 1 cut(s) | 444 |
| Kzo9I | GATC | 4 cut(s) | 1081, 1849, 1879, 2335 |
| LguI | GCTCTTC | 1 cut(s) | 257 |
| LmnI | GCTCC | 8 cut(s) | 578, 790, 1051, 1463, 1606, 1829, 1837, 1955 |
| Lsp1109I | GCAGC | 8 cut(s) | 731, 748, 821, 843, 844, 849, 1079, 1744 |
| LweI | GCATC | 5 cut(s) | 673, 719, 859, 911, 1198 |
| MabI | ACCWGGT | 1 cut(s) | 272 |
| MaeI | CTAG | 7 cut(s) | 375, 774, 795, 854, 1377, 1481, 1793 |
| MaeII | ACGT | 1 cut(s) | 522 |
| MaeIII | GTNAC | 6 cut(s) | 61, 581, 1190, 1235, 1777, 2095 |
| MalI | GATC | 4 cut(s) | 1083, 1851, 1881, 2337 |
| MboI | GATC | 4 cut(s) | 1081, 1849, 1879, 2335 |
| MfeI | CAATTG | 1 cut(s) | 1434 |
| MflI | RGATCY | 1 cut(s) | 1879 |
| MhlI | GDGCHC | 4 cut(s) | 50, 604, 787, 1834 |
| MlyI | GAGTC | 5 cut(s) | 292, 442, 497, 1232, 1759 |
| MmeI | TCCRAC | 4 cut(s) | 362, 515, 1520, 1766 |
| MroXI | GAANNNNTTC | 2 cut(s) | 424, 1166 |
| MspA1I | CMGCKG | 2 cut(s) | 722, 834 |
| MspI | CCGG | 4 cut(s) | 73, 1227, 2111, 2151 |
| MunI | CAATTG | 1 cut(s) | 1434 |
| Mva1269I | GAATGC | 1 cut(s) | 1538 |
| MvaI | CCWGG | 8 cut(s) | 274, 327, 1051, 1109, 1605, 1627, 1775, 1902 |
| NciI | CCSGG | 2 cut(s) | 73, 1228 |
| NcoI | CCATGG | 1 cut(s) | 1953 |
| NdeII | GATC | 4 cut(s) | 1081, 1849, 1879, 2335 |
| NlaIV | GGNNCC | 5 cut(s) | 202, 1558, 1631, 1684, 1881 |
| NmuCI | GTSAC | 2 cut(s) | 1190, 1235 |
| NspI | RCATGY | 2 cut(s) | 2007, 2297 |
| NspV | TTCGAA | 1 cut(s) | 2320 |
| PagI | TCATGA | 1 cut(s) | 2059 |
| PasI | CCCWGGG | 1 cut(s) | 1108 |
| PceI | AGGCCT | 1 cut(s) | 119 |
| PciSI | GCTCTTC | 1 cut(s) | 257 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 676 |
| PctI | GAATGC | 1 cut(s) | 1538 |
| PdmI | GAANNNNTTC | 2 cut(s) | 424, 1166 |
| PfeI | GAWTC | 7 cut(s) | 297, 462, 984, 1279, 1676, 1922, 2256 |
| PkrI | GCNGC | 8 cut(s) | 721, 763, 833, 836, 839, 859, 1069, 1759 |
| PleI | GAGTC | 5 cut(s) | 291, 441, 496, 1232, 1758 |
| PpsI | GAGTC | 5 cut(s) | 291, 441, 496, 1232, 1758 |
| PpuMI | RGGWCCY | 2 cut(s) | 201, 1556 |
| Psp124BI | GAGCTC | 1 cut(s) | 1834 |
| Psp5II | RGGWCCY | 2 cut(s) | 201, 1556 |
| Psp6I | CCWGG | 8 cut(s) | 272, 325, 1049, 1107, 1603, 1625, 1773, 1900 |
| PspFI | CCCAGC | 2 cut(s) | 446, 1988 |
| PspGI | CCWGG | 8 cut(s) | 272, 325, 1049, 1107, 1603, 1625, 1773, 1900 |
| PspN4I | GGNNCC | 5 cut(s) | 202, 1558, 1631, 1684, 1881 |
| PspPI | GGNCC | 6 cut(s) | 201, 483, 1016, 1556, 1629, 2108 |
| PspPPI | RGGWCCY | 2 cut(s) | 201, 1556 |
| PstI | CTGCAG | 2 cut(s) | 721, 839 |
| PsuI | RGATCY | 1 cut(s) | 1879 |
| PvuII | CAGCTG | 2 cut(s) | 722, 834 |
| RsaI | GTAC | 3 cut(s) | 649, 700, 826 |
| RsaNI | GTAC | 3 cut(s) | 648, 699, 825 |
| Rsr2I | CGGWCCG | 1 cut(s) | 2108 |
| RsrII | CGGWCCG | 1 cut(s) | 2108 |
| SacI | GAGCTC | 1 cut(s) | 1834 |
| SapI | GCTCTTC | 1 cut(s) | 257 |
| SatI | GCNGC | 8 cut(s) | 720, 762, 832, 835, 838, 858, 1068, 1758 |
| Sau3AI | GATC | 4 cut(s) | 1081, 1849, 1879, 2335 |
| Sau96I | GGNCC | 6 cut(s) | 201, 483, 1016, 1556, 1629, 2108 |
| SchI | GAGTC | 5 cut(s) | 292, 442, 497, 1232, 1759 |
| SduI | GDGCHC | 4 cut(s) | 50, 604, 787, 1834 |
| SexAI | ACCWGGT | 1 cut(s) | 272 |
| SfaNI | GCATC | 5 cut(s) | 673, 719, 859, 911, 1198 |
| SfcI | CTRYAG | 3 cut(s) | 717, 835, 972 |
| SfuI | TTCGAA | 1 cut(s) | 2320 |
| SinI | GGWCC | 5 cut(s) | 201, 1016, 1556, 1629, 2108 |
| SseBI | AGGCCT | 1 cut(s) | 119 |
| SsiI | CCGC | 2 cut(s) | 764, 1869 |
| SspI | AATATT | 1 cut(s) | 1615 |
| SspMI | CTAG | 7 cut(s) | 375, 774, 795, 854, 1377, 1481, 1793 |
| SstI | GAGCTC | 1 cut(s) | 1834 |
| StuI | AGGCCT | 1 cut(s) | 119 |
| StyI | CCWWGG | 1 cut(s) | 1953 |
| TaiI | ACGT | 1 cut(s) | 525 |
| TaqI | TCGA | 4 cut(s) | 89, 1297, 2015, 2320 |
| TaqII | GACCGA | 2 cut(s) | 660, 2096 |
| TatI | WGTACW | 1 cut(s) | 647 |
| TfiI | GAWTC | 7 cut(s) | 297, 462, 984, 1279, 1676, 1922, 2256 |
| TscAI | CASTG | 7 cut(s) | 50, 478, 584, 1066, 1228, 1338, 2316 |
| TseFI | GTSAC | 2 cut(s) | 1190, 1235 |
| TseI | GCWGC | 8 cut(s) | 719, 761, 831, 834, 837, 857, 1067, 1757 |
| Tsp45I | GTSAC | 2 cut(s) | 1190, 1235 |
| TspDTI | ATGAA | 9 cut(s) | 20, 275, 326, 639, 1230, 1352, 1815, 2014, 2048 |
| TspGWI | ACGGA | 1 cut(s) | 1842 |
| TspRI | CASTG | 7 cut(s) | 50, 478, 584, 1066, 1228, 1338, 2316 |
| VneI | GTGCAC | 1 cut(s) | 600 |
| VpaK11BI | GGWCC | 5 cut(s) | 201, 1016, 1556, 1629, 2108 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1402 |
| XapI | RAATTY | 8 cut(s) | 385, 420, 477, 632, 665, 1009, 1039, 2021 |
| XbaI | TCTAGA | 1 cut(s) | 1792 |
| XceI | RCATGY | 2 cut(s) | 2007, 2297 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1981 |
| XmiI | GTMKAC | 3 cut(s) | 807, 1145, 2124 |
| XmnI | GAANNNNTTC | 2 cut(s) | 424, 1166 |
| XspI | CTAG | 7 cut(s) | 375, 774, 795, 854, 1377, 1481, 1793 |
| ZraI | GACGTC | 1 cut(s) | 523 |