Rh6AG154000

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014653
Physical Location & Seq
Forward (+)
23990465 .. 23997244
6780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG154000.1

Sequence Viewer

Length: 1899 bp
ATGGACACTGGAATTTGGCCCGAGTCTCAAAGCTTTAGAGATGAGGGTATGGGAGACATTCCATCTCGTTGGAGAGGCATATGTCAAGAAGGAGAAAGGTTCAATCACTCCCATTGCAACAGGAAGATTATTGGTGCACGCTGGTATATTAAAGGATATGAAGCTGAATTTGGAAAGATAGATACAAGTGATGGGTTTGAATTTCTATCTCCCCGAGATGCTTCAGGACATGGTACTCACACATCATCCACTGCAGCCGGTGGTTTTATAGAAAATGCAAGCTTTATGGGACTTGCTTCAGGATTGGCAAGAGGAGGTGCTCCATCATCTTGGTTAGCTATTTACAAAGTTTGTTGGGCTACTGGTGGCTGCAGCTCAGCAGACATACTTGCTGCATTTGATGATGCAATTTTTGATGGTGTGGACGTGCTTTCGGCATCTCTAGGTTCTCCGCCTCCACTTGCTTCTTATGTTGAGGATGTTGTGTCCATTGGTTCTTTTCATGCTGTGGCCAAAGGAATCTCAGTTGTATGCTCTGCTGGAAACTCTGGCCCATACTCTCAAACTATCATAAATTCTGCTCCTTGGATATTCACTGTTGCAGCAAGCACTATCGACAGGGCTTTTCCTACTGCAATCTCCCTGGGAAACAATCAAACTGTAGTGGGTCAGGGTTTGTATACAGGGATGGATGTGAACAAGTTCTACCCTCTTGTGTATGGACAAGACATTGCATCTGTTGATGCAGACGAAGACAGTGCCGGGAGCTGTGAGTTGGGAACTCTAAATGGTACTTTAGCAAACGGAAAATTTGTTCTTTGTTTCCAATCTCGATCACAGAGGTCATCCACTGCTGCACAGAGAACTGTAATGAGTGCTGGGGGTGCCGGTCTCATTTTTGCTCAGTTTCCCAGCAAGGATGTTTCTTTGTCCTCGGATAGTCTACCGTGTGTTCAAATTGACTTTGCAATCGGCACATATATTCTCACATATATTGGAGCAACCAGAAATCCAATAGTCAAGTTAAACCCTACAAAAACAGTTTTGGAACAGCAGATATCCCCAGAAGTGGCATTCTTCTCTGCTCGAGGACCCAATTCTGTCTCTCCTTCAGTACTGAAGCCCGATATTGCTGCTCCTGGTGTGAATATATTGTCCTCTTGGTCCCCTGCTTATTCACTATTACAACATCAACCATCTCCAGTCATCTTTAAAATTGAATCAGGAACTTCCATGGCTTGTCCACATATATCTGGTATAGTGGCTCTTCTTAAAAGCATCCACCCAATATGGAGCCCTGCAGCAATAAAGTCTGCCCTTATTACAACAGCCTCTTTAGAAGATCAATACGGTCAAAGTATCGAGGCTGAGGGCGCTCCTCAGAAGAAAGCTGATCCATTTGACTATGGTGGTGGACATGTCAATCCTAACAAAGCCATAGCCCCCGGGCTCATTTATGATATAGAAACCTCAGATTACATCCGTTTTCTTTGCTCCATGGGATATAACAATTCAGCCATCAGCGCAATCGCAGGAGCTAGCATCACATGCCATAAATCGACCAATGTCCAAGTTAATCTCAACCTGCCTTCCATCACCATTCATGAGCTCAAGGAAAGGATGACAGTATCAAGAACAGTCACAAATGTTGGTCCAGTTAATTCTATCTACATTGCTCGCGTTCAAACACCTGCCGGCGTGTCTATTAAAGTTAAGCCATCGGCTTTGTTGTTCAACTCTACTGCAAAGAAGCTGGAGTTCAAGGTTGTTTTTCGTCCCCTGCTAAAAGTTGCAGGAAGATTTTCTTTTGGAAATCTGTACTGGGAAGATGGTTTTCATGTAGTGAGGATACCTCTAACAGTGAGAACTGTCCTTGATGATTTTTACGCAGAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

632

Amino Acids

67.27

Weight (kDa)

6.08

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 64 - 449 1e-41 Subtilase family
fn3_6 PF17766 526 - 621 7.3e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014653)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10210 FvH4_2g10210 FvH4_2g10210
malus_domestica MD05G1055900.v1.1
prunus_persica Prupe.8G086300_v2.0.a1
pyrus_communis pycom05g04760
rosa_chinensis RchiOBHm_Chr6g0267441
rosa_laevigata RLG00000014004
rosa_multiflora Rmu_sc0001068.1_g000015 Rmu_sc0006674.1_g000011
rosa_roxburghii Rroxscaffold_7G00200780
rosa_rugosa Rorug06G0032600 Rorug06G0032600
rosa_samantha Rh6AG154000 Rh6BG154100 Rh6CG149800
rosa_wichuraiana Rw6G013220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1699
Acc36I ACCTGC 2 cut(s) 1593, 1699
AccB1I GGYRCC 1 cut(s) 884
AccI GTMKAC 2 cut(s) 680, 943
AccII CGCG 1 cut(s) 1680
AciI CCGC 1 cut(s) 452
AclWI GGATC 1 cut(s) 1388
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 5 cut(s) 12, 167, 200, 574, 809
AcuI CTGAAG 4 cut(s) 207, 282, 1095, 1139
AfaI GTAC 4 cut(s) 235, 793, 1116, 1820
AfiI CCNNNNNNNGG 1 cut(s) 1069
AflIII ACRYGT 1 cut(s) 1417
AgsI TTSAA 7 cut(s) 103, 200, 956, 1220, 1685, 1735, 1762
AjiI CACGTC 1 cut(s) 427
AjnI CCWGG 2 cut(s) 642, 1138
AjuI GAANNNNNNNTTGG 2 cut(s) 153, 185
AloI GAACNNNNNNTCC 2 cut(s) 798, 830
Alw21I GWGCWC 3 cut(s) 139, 322, 1611
Alw26I GTCTC 4 cut(s) 30, 48, 896, 1108
Alw44I GTGCAC 1 cut(s) 135
AlwI GGATC 1 cut(s) 1388
Ama87I CYCGRG 4 cut(s) 20, 213, 1086, 1445
AoxI GGCC 3 cut(s) 17, 510, 550
ApaLI GTGCAC 1 cut(s) 135
ApeKI GCWGC 8 cut(s) 254, 369, 372, 392, 602, 854, 1133, 1301
ApoI RAATTY 5 cut(s) 12, 167, 200, 574, 809
Asp700I GAANNNNTTC 2 cut(s) 701, 1801
AspLEI GCGC 2 cut(s) 1376, 1526
AspS9I GGNCC 5 cut(s) 18, 551, 1091, 1164, 1652
AsuC2I CCSGG 3 cut(s) 763, 1446, 1447
AsuHPI GGTGA 1 cut(s) 1588
AsuNHI GCTAGC 1 cut(s) 1538
AvaI CYCGRG 4 cut(s) 20, 213, 1086, 1445
AvaII GGWCC 3 cut(s) 1091, 1164, 1652
BaeGI GKGCMC 1 cut(s) 139
BalI TGGCCA 1 cut(s) 512
BanI GGYRCC 1 cut(s) 884
BanII GRGCYC 3 cut(s) 1298, 1452, 1611
BbsI GAAGAC 1 cut(s) 759
Bbv12I GWGCWC 3 cut(s) 139, 322, 1611
BbvCI CCTCAGC 1 cut(s) 1368
BbvI GCAGC 8 cut(s) 266, 356, 379, 384, 614, 841, 1120, 1313
BcgI CGANNNNNNTGC 4 cut(s) 740, 774, 1115, 1149
BciT130I CCWGG 2 cut(s) 644, 1140
BciVI GTATCC 1 cut(s) 1842
BcnI CCSGG 3 cut(s) 763, 1446, 1447
BcoDI GTCTC 4 cut(s) 30, 48, 896, 1108
BfaI CTAG 2 cut(s) 443, 1539
BfmI CTRYAG 4 cut(s) 252, 370, 660, 1299
BfoI RGCGCY 1 cut(s) 1377
BfuAI ACCTGC 2 cut(s) 1593, 1699
BfuI GTATCC 1 cut(s) 1842
BisI GCNGC 8 cut(s) 255, 370, 373, 393, 603, 855, 1134, 1302
BlpI GCTNAGC 1 cut(s) 376
BlsI GCNGC 8 cut(s) 256, 371, 374, 394, 604, 856, 1135, 1303
BmcAI AGTACT 1 cut(s) 1116
Bme1390I CCNGG 5 cut(s) 644, 763, 1140, 1446, 1447
Bme18I GGWCC 3 cut(s) 1091, 1164, 1652
BmeT110I CYCGRG 4 cut(s) 20, 213, 1086, 1445
BmgBI CACGTC 1 cut(s) 427
BmgT120I GGNCC 5 cut(s) 18, 551, 1091, 1164, 1652
BmiI GGNNCC 4 cut(s) 886, 1093, 1166, 1295
BmrFI CCNGG 5 cut(s) 644, 763, 1140, 1446, 1447
BmrI ACTGGG 1 cut(s) 1831
BmsI GCATC 7 cut(s) 208, 394, 446, 733, 743, 1287, 1551
BmtI GCTAGC 1 cut(s) 1542
BmuI ACTGGG 1 cut(s) 1831
BoxI GACNNNNGTC 1 cut(s) 1565
BpiI GAAGAC 1 cut(s) 759
BplI GAGNNNNNCTC 2 cut(s) 1837, 1869
BpmI CTGGAG 2 cut(s) 1185, 1775
Bpu10I CCTNAGC 1 cut(s) 1368
Bpu1102I GCTNAGC 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 1595
BpuMI CCSGG 3 cut(s) 763, 1446, 1447
BsaI GGTCTC 1 cut(s) 896
BsaJI CCNNGG 8 cut(s) 584, 642, 643, 933, 1233, 1444, 1445, 1497
Bsc4I CCNNNNNNNGG 1 cut(s) 1069
Bse118I RCCGGY 3 cut(s) 257, 887, 1694
Bse1I ACTGG 5 cut(s) 13, 367, 1202, 1655, 1826
Bse3DI GCAATG 3 cut(s) 112, 729, 1671
BseBI CCWGG 2 cut(s) 644, 1140
BseDI CCNNGG 8 cut(s) 584, 642, 643, 933, 1233, 1444, 1445, 1497
BseGI GGATG 9 cut(s) 245, 484, 693, 697, 845, 925, 1278, 1479, 1626
BseLI CCNNNNNNNGG 1 cut(s) 1069
BseMI GCAATG 3 cut(s) 112, 729, 1671
BseMII CTCAG 6 cut(s) 390, 537, 917, 1359, 1394, 1485
BseNI ACTGG 5 cut(s) 13, 367, 1202, 1655, 1826
BseRI GAGGAG 2 cut(s) 327, 1368
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 8 cut(s) 266, 356, 379, 384, 614, 841, 1120, 1313
BseYI CCCAGC 2 cut(s) 878, 911
BsgI GTGCAG 1 cut(s) 840
Bsh1236I CGCG 1 cut(s) 1680
BshFI GGCC 3 cut(s) 19, 512, 552
BshNI GGYRCC 1 cut(s) 884
BsiHKAI GWGCWC 3 cut(s) 139, 322, 1611
BsiHKCI CYCGRG 4 cut(s) 20, 213, 1086, 1445
BsiSI CCGG 5 cut(s) 258, 762, 888, 1446, 1695
BslFI GGGAC 3 cut(s) 303, 1150, 1761
BslI CCNNNNNNNGG 1 cut(s) 1069
BsmAI GTCTC 4 cut(s) 30, 48, 896, 1108
BsmFI GGGAC 3 cut(s) 303, 1150, 1761
BsmI GAATGC 1 cut(s) 1073
BsnI GGCC 3 cut(s) 19, 512, 552
Bso31I GGTCTC 1 cut(s) 896
BsoBI CYCGRG 4 cut(s) 20, 213, 1086, 1445
Bsp1286I GDGCHC 5 cut(s) 139, 322, 1298, 1452, 1611
Bsp143I GATC 3 cut(s) 833, 1342, 1393
Bsp1720I GCTNAGC 1 cut(s) 376
Bsp19I CCATGG 2 cut(s) 1233, 1497
BspACI CCGC 1 cut(s) 452
BspANI GGCC 3 cut(s) 19, 512, 552
BspCNI CTCAG 6 cut(s) 389, 536, 916, 1360, 1393, 1484
BspFNI CGCG 1 cut(s) 1680
BspHI TCATGA 1 cut(s) 1603
BspLI GGNNCC 4 cut(s) 886, 1093, 1166, 1295
BspMAI CTGCAG 3 cut(s) 256, 374, 1303
BspMI ACCTGC 2 cut(s) 1593, 1699
BspOI GCTAGC 1 cut(s) 1542
BspPI GGATC 1 cut(s) 1388
BspQI GCTCTTC 1 cut(s) 1272
BspT107I GGYRCC 1 cut(s) 884
BspTNI GGTCTC 1 cut(s) 896
BsrDI GCAATG 3 cut(s) 112, 729, 1671
BsrFI RCCGGY 3 cut(s) 257, 887, 1694
BsrI ACTGG 5 cut(s) 13, 367, 1202, 1655, 1826
BssAI RCCGGY 3 cut(s) 257, 887, 1694
BssECI CCNNGG 8 cut(s) 584, 642, 643, 933, 1233, 1444, 1445, 1497
BssMI GATC 3 cut(s) 833, 1342, 1393
BssNAI GTATAC 1 cut(s) 681
BssT1I CCWWGG 3 cut(s) 584, 1233, 1497
Bst1107I GTATAC 1 cut(s) 681
Bst2UI CCWGG 2 cut(s) 644, 1140
Bst6I CTCTTC 1 cut(s) 1272
BstAPI GCANNNNNTGC 1 cut(s) 1548
BstC8I GCNNGC 6 cut(s) 139, 280, 607, 1540, 1678, 1696
BstDEI CTNAG 6 cut(s) 376, 523, 903, 1368, 1380, 1471
BstDSI CCRYGG 2 cut(s) 1233, 1497
BstF5I GGATG 9 cut(s) 245, 484, 693, 697, 845, 925, 1278, 1479, 1626
BstFNI CGCG 1 cut(s) 1680
BstH2I RGCGCY 1 cut(s) 1377
BstHHI GCGC 2 cut(s) 1376, 1526
BstKTI GATC 3 cut(s) 836, 1345, 1396
BstMAI GTCTC 4 cut(s) 30, 48, 896, 1108
BstMBI GATC 3 cut(s) 833, 1342, 1393
BstMWI GCNNNNNNNGC 4 cut(s) 884, 1373, 1523, 1548
BstNI CCWGG 2 cut(s) 644, 1140
BstNSI RCATGY 2 cut(s) 1421, 1551
BstPAI GACNNNNGTC 1 cut(s) 1565
BstSCI CCNGG 5 cut(s) 642, 761, 1138, 1444, 1445
BstSFI CTRYAG 4 cut(s) 252, 370, 660, 1299
BstSLI GKGCMC 1 cut(s) 139
BstUI CGCG 1 cut(s) 1680
BstV1I GCAGC 8 cut(s) 266, 356, 379, 384, 614, 841, 1120, 1313
BstV2I GAAGAC 1 cut(s) 759
BstXI CCANNNNNNTGG 2 cut(s) 69, 330
BstZ17I GTATAC 1 cut(s) 681
BsuI GTATCC 1 cut(s) 1842
BsuRI GGCC 3 cut(s) 19, 512, 552
BtgI CCRYGG 2 cut(s) 1233, 1497
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 9 cut(s) 245, 484, 693, 697, 845, 925, 1278, 1479, 1626
BtsI GCAGTG 2 cut(s) 249, 849
BtsIMutI CAGTG 6 cut(s) 6, 249, 594, 763, 849, 1866
BveI ACCTGC 2 cut(s) 1593, 1699
Cac8I GCNNGC 6 cut(s) 139, 280, 607, 1540, 1678, 1696
CciI TCATGA 1 cut(s) 1603
CfoI GCGC 2 cut(s) 1376, 1526
Cfr10I RCCGGY 3 cut(s) 257, 887, 1694
Cfr13I GGNCC 5 cut(s) 18, 551, 1091, 1164, 1652
Cfr9I CCCGGG 1 cut(s) 1445
Csp6I GTAC 4 cut(s) 234, 792, 1115, 1819
CviAII CATG 9 cut(s) 230, 503, 1234, 1418, 1498, 1548, 1604, 1838, 1896
CviQI GTAC 4 cut(s) 234, 792, 1115, 1819
DdeI CTNAG 6 cut(s) 376, 523, 903, 1368, 1380, 1471
DpnI GATC 3 cut(s) 835, 1344, 1395
DpnII GATC 3 cut(s) 833, 1342, 1393
DraI TTTAAA 1 cut(s) 1213
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 1272
EarI CTCTTC 1 cut(s) 1272
EciI GGCGGA 1 cut(s) 441
Ecl136II GAGCTC 1 cut(s) 1609
Eco130I CCWWGG 3 cut(s) 584, 1233, 1497
Eco24I GRGCYC 3 cut(s) 1298, 1452, 1611
Eco31I GGTCTC 1 cut(s) 896
Eco32I GATATC 1 cut(s) 1059
Eco47I GGWCC 3 cut(s) 1091, 1164, 1652
Eco53kI GAGCTC 1 cut(s) 1609
Eco57I CTGAAG 4 cut(s) 207, 282, 1095, 1139
Eco88I CYCGRG 4 cut(s) 20, 213, 1086, 1445
EcoICRI GAGCTC 1 cut(s) 1609
EcoO109I RGGNCCY 1 cut(s) 1091
EcoRII CCWGG 2 cut(s) 642, 1138
EcoRV GATATC 1 cut(s) 1059
EcoT14I CCWWGG 3 cut(s) 584, 1233, 1497
EcoT38I GRGCYC 3 cut(s) 1298, 1452, 1611
ErhI CCWWGG 3 cut(s) 584, 1233, 1497
FaeI CATG 9 cut(s) 233, 506, 1237, 1421, 1501, 1551, 1607, 1841, 1899
FalI AAGNNNNNCTT 2 cut(s) 1789, 1821
FaqI GGGAC 3 cut(s) 303, 1150, 1761
FatI CATG 9 cut(s) 229, 502, 1233, 1417, 1497, 1547, 1603, 1837, 1895
FauNDI CATATG 1 cut(s) 80
FblI GTMKAC 2 cut(s) 680, 943
Fnu4HI GCNGC 8 cut(s) 255, 370, 373, 393, 603, 855, 1134, 1302
FokI GGATG 9 cut(s) 232, 491, 700, 704, 832, 932, 1265, 1466, 1633
FriOI GRGCYC 3 cut(s) 1298, 1452, 1611
Fsp4HI GCNGC 8 cut(s) 255, 370, 373, 393, 603, 855, 1134, 1302
FspBI CTAG 2 cut(s) 443, 1539
GlaI GCGC 2 cut(s) 1375, 1525
GluI GCNGC 8 cut(s) 255, 370, 373, 393, 603, 855, 1134, 1302
GsaI CCCAGC 2 cut(s) 882, 915
GsuI CTGGAG 2 cut(s) 1185, 1775
HaeII RGCGCY 1 cut(s) 1377
HaeIII GGCC 3 cut(s) 19, 512, 552
HapII CCGG 5 cut(s) 258, 762, 888, 1446, 1695
HhaI GCGC 2 cut(s) 1376, 1526
Hin1II CATG 9 cut(s) 233, 506, 1237, 1421, 1501, 1551, 1607, 1841, 1899
Hin6I GCGC 2 cut(s) 1374, 1524
HinP1I GCGC 2 cut(s) 1374, 1524
HindIII AAGCTT 2 cut(s) 31, 280
HinfI GANTC 3 cut(s) 23, 519, 1220
HpaII CCGG 5 cut(s) 258, 762, 888, 1446, 1695
HphI GGTGA 1 cut(s) 1588
Hpy166II GTNNAC 7 cut(s) 137, 424, 681, 697, 944, 1244, 1415
Hpy188I TCNGA 3 cut(s) 937, 1383, 1474
Hpy188III TCNNGA 7 cut(s) 86, 225, 300, 831, 1224, 1604, 1632
Hpy8I GTNNAC 7 cut(s) 137, 424, 681, 697, 944, 1244, 1415
HpyAV CCTTC 3 cut(s) 83, 1119, 1599
HpyCH4IV ACGT 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 4 cut(s) 884, 1373, 1523, 1548
HpyF3I CTNAG 6 cut(s) 376, 523, 903, 1368, 1380, 1471
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 9 cut(s) 233, 506, 1237, 1421, 1501, 1551, 1607, 1841, 1899
HspAI GCGC 2 cut(s) 1374, 1524
KroI GCCGGC 1 cut(s) 1694
KroNI GCCGGC 1 cut(s) 1696
Kzo9I GATC 3 cut(s) 833, 1342, 1393
LguI GCTCTTC 1 cut(s) 1272
LmnI GCTCC 9 cut(s) 325, 586, 765, 998, 1141, 1293, 1381, 1499, 1535
Lsp1109I GCAGC 8 cut(s) 266, 356, 379, 384, 614, 841, 1120, 1313
LweI GCATC 7 cut(s) 208, 394, 446, 733, 743, 1287, 1551
MaeI CTAG 2 cut(s) 443, 1539
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 1 cut(s) 1639
MalI GATC 3 cut(s) 835, 1344, 1395
MboI GATC 3 cut(s) 833, 1342, 1393
MboII GAAGA 8 cut(s) 136, 764, 1069, 1259, 1352, 1396, 1809, 1838
MhlI GDGCHC 5 cut(s) 139, 322, 1298, 1452, 1611
MlsI TGGCCA 1 cut(s) 512
MluNI TGGCCA 1 cut(s) 512
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 50
Mox20I TGGCCA 1 cut(s) 512
MroNI GCCGGC 1 cut(s) 1694
MroXI GAANNNNTTC 2 cut(s) 701, 1801
MscI TGGCCA 1 cut(s) 512
MseI TTAA 8 cut(s) 150, 1025, 1212, 1272, 1575, 1659, 1707, 1713
Msp20I TGGCCA 1 cut(s) 512
MspI CCGG 5 cut(s) 258, 762, 888, 1446, 1695
MspR9I CCNGG 5 cut(s) 644, 763, 1140, 1446, 1447
Mva1269I GAATGC 1 cut(s) 1073
MvaI CCWGG 2 cut(s) 644, 1140
MvnI CGCG 1 cut(s) 1680
MwoI GCNNNNNNNGC 4 cut(s) 884, 1373, 1523, 1548
NaeI GCCGGC 1 cut(s) 1696
NciI CCSGG 3 cut(s) 763, 1446, 1447
NcoI CCATGG 2 cut(s) 1233, 1497
NdeI CATATG 1 cut(s) 80
NdeII GATC 3 cut(s) 833, 1342, 1393
NgoMIV GCCGGC 1 cut(s) 1694
NheI GCTAGC 1 cut(s) 1538
NlaIII CATG 9 cut(s) 233, 506, 1237, 1421, 1501, 1551, 1607, 1841, 1899
NlaIV GGNNCC 4 cut(s) 886, 1093, 1166, 1295
NmuCI GTSAC 1 cut(s) 1639
NspI RCATGY 2 cut(s) 1421, 1551
PaeR7I CTCGAG 1 cut(s) 1086
PagI TCATGA 1 cut(s) 1603
PaqCI CACCTGC 1 cut(s) 1699
PasI CCCWGGG 1 cut(s) 643
PciI ACATGT 1 cut(s) 1417
PciSI GCTCTTC 1 cut(s) 1272
PctI GAATGC 1 cut(s) 1073
PdiI GCCGGC 1 cut(s) 1696
PdmI GAANNNNTTC 2 cut(s) 701, 1801
PfeI GAWTC 2 cut(s) 519, 1220
PkrI GCNGC 8 cut(s) 256, 371, 374, 394, 604, 856, 1135, 1303
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 1091
PscI ACATGT 1 cut(s) 1417
PshAI GACNNNNGTC 1 cut(s) 1565
Psp124BI GAGCTC 1 cut(s) 1611
Psp5II RGGWCCY 1 cut(s) 1091
Psp6I CCWGG 2 cut(s) 642, 1138
PspFI CCCAGC 2 cut(s) 878, 911
PspGI CCWGG 2 cut(s) 642, 1138
PspN4I GGNNCC 4 cut(s) 886, 1093, 1166, 1295
PspPI GGNCC 5 cut(s) 18, 551, 1091, 1164, 1652
PspPPI RGGWCCY 1 cut(s) 1091
PspXI VCTCGAGB 1 cut(s) 1086
PsrI GAACNNNNNNTAC 2 cut(s) 689, 721
PstI CTGCAG 3 cut(s) 256, 374, 1303
RsaI GTAC 4 cut(s) 235, 793, 1116, 1820
RsaNI GTAC 4 cut(s) 234, 792, 1115, 1819
SacI GAGCTC 1 cut(s) 1611
SapI GCTCTTC 1 cut(s) 1272
SaqAI TTAA 8 cut(s) 150, 1025, 1212, 1272, 1575, 1659, 1707, 1713
SatI GCNGC 8 cut(s) 255, 370, 373, 393, 603, 855, 1134, 1302
Sau3AI GATC 3 cut(s) 833, 1342, 1393
Sau96I GGNCC 5 cut(s) 18, 551, 1091, 1164, 1652
ScaI AGTACT 1 cut(s) 1116
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 5 cut(s) 644, 763, 1140, 1446, 1447
SduI GDGCHC 5 cut(s) 139, 322, 1298, 1452, 1611
SfaNI GCATC 7 cut(s) 208, 394, 446, 733, 743, 1287, 1551
SfcI CTRYAG 4 cut(s) 252, 370, 660, 1299
Sfr274I CTCGAG 1 cut(s) 1086
SinI GGWCC 3 cut(s) 1091, 1164, 1652
SlaI CTCGAG 1 cut(s) 1086
SmaI CCCGGG 1 cut(s) 1447
SmlI CTYRAG 2 cut(s) 1086, 1610
SmoI CTYRAG 2 cut(s) 1086, 1610
SsiI CCGC 1 cut(s) 452
SspMI CTAG 2 cut(s) 443, 1539
SstI GAGCTC 1 cut(s) 1611
StyD4I CCNGG 5 cut(s) 642, 761, 1138, 1444, 1445
StyI CCWWGG 3 cut(s) 584, 1233, 1497
TaiI ACGT 1 cut(s) 429
TaqI TCGA 5 cut(s) 615, 832, 1087, 1362, 1559
TatI WGTACW 2 cut(s) 1114, 1818
TfiI GAWTC 2 cut(s) 519, 1220
Tru1I TTAA 8 cut(s) 150, 1025, 1212, 1272, 1575, 1659, 1707, 1713
Tru9I TTAA 8 cut(s) 150, 1025, 1212, 1272, 1575, 1659, 1707, 1713
TscAI CASTG 6 cut(s) 13, 256, 601, 763, 856, 1866
TseFI GTSAC 1 cut(s) 1639
TseI GCWGC 8 cut(s) 254, 369, 372, 392, 602, 854, 1133, 1301
Tsp45I GTSAC 1 cut(s) 1639
TspDTI ATGAA 4 cut(s) 174, 491, 1592, 1826
TspGWI ACGGA 2 cut(s) 819, 1472
TspMI CCCGGG 1 cut(s) 1445
TspRI CASTG 6 cut(s) 13, 256, 601, 763, 856, 1866
VneI GTGCAC 1 cut(s) 135
VpaK11BI GGWCC 3 cut(s) 1091, 1164, 1652
XapI RAATTY 5 cut(s) 12, 167, 200, 574, 809
XceI RCATGY 2 cut(s) 1421, 1551
XhoI CTCGAG 1 cut(s) 1086
XmaI CCCGGG 1 cut(s) 1445
XmiI GTMKAC 2 cut(s) 680, 943
XmnI GAANNNNTTC 2 cut(s) 701, 1801
XspI CTAG 2 cut(s) 443, 1539
ZrmI AGTACT 1 cut(s) 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.