Subtilase family
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_2g10210 FvH4_2g10210 FvH4_2g10210 |
| malus_domestica | MD05G1055900.v1.1 |
| prunus_persica | Prupe.8G086300_v2.0.a1 |
| pyrus_communis | pycom05g04760 |
| rosa_chinensis | RchiOBHm_Chr6g0267441 |
| rosa_laevigata | RLG00000014004 |
| rosa_multiflora | Rmu_sc0001068.1_g000015 Rmu_sc0006674.1_g000011 |
| rosa_roxburghii | Rroxscaffold_7G00200780 |
| rosa_rugosa | Rorug06G0032600 Rorug06G0032600 |
| rosa_samantha | Rh6AG154000 Rh6BG154100 Rh6CG149800 |
| rosa_wichuraiana | Rw6G013220 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2206 |
| Acc36I | ACCTGC | 2 cut(s) | 2100, 2206 |
| AccB1I | GGYRCC | 1 cut(s) | 1391 |
| AccI | GTMKAC | 3 cut(s) | 441, 1187, 1450 |
| AciI | CCGC | 3 cut(s) | 165, 273, 959 |
| AclWI | GGATC | 1 cut(s) | 1895 |
| AcoI | YGGCCR | 1 cut(s) | 1017 |
| AcsI | RAATTY | 6 cut(s) | 519, 674, 707, 1026, 1081, 1316 |
| AcuI | CTGAAG | 4 cut(s) | 77, 714, 789, 1646 |
| AfaI | GTAC | 6 cut(s) | 293, 366, 742, 1300, 1623, 2327 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 58, 95, 217, 1576 |
| AflIII | ACRYGT | 1 cut(s) | 1924 |
| AgsI | TTSAA | 9 cut(s) | 42, 471, 610, 707, 1463, 1727, 2192, 2242, 2269 |
| AjiI | CACGTC | 1 cut(s) | 934 |
| AjnI | CCWGG | 4 cut(s) | 94, 1149, 1645, 1951 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 660, 692 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1305, 1337 |
| Alw21I | GWGCWC | 3 cut(s) | 646, 829, 2118 |
| Alw26I | GTCTC | 5 cut(s) | 234, 537, 555, 1403, 1615 |
| Alw44I | GTGCAC | 1 cut(s) | 642 |
| AlwI | GGATC | 1 cut(s) | 1895 |
| Ama87I | CYCGRG | 4 cut(s) | 527, 720, 1593, 2184 |
| AoxI | GGCC | 4 cut(s) | 49, 524, 1017, 1057 |
| ApaLI | GTGCAC | 1 cut(s) | 642 |
| ApeKI | GCWGC | 9 cut(s) | 323, 761, 876, 879, 899, 1109, 1361, 1640, 1808 |
| ApoI | RAATTY | 6 cut(s) | 519, 674, 707, 1026, 1081, 1316 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 285, 1208, 2308 |
| AspLEI | GCGC | 2 cut(s) | 1883, 2033 |
| AspS9I | GGNCC | 5 cut(s) | 525, 1058, 1598, 1671, 2159 |
| AsuC2I | CCSGG | 2 cut(s) | 369, 1270 |
| AsuHPI | GGTGA | 2 cut(s) | 100, 2095 |
| AsuNHI | GCTAGC | 1 cut(s) | 2045 |
| AvaI | CYCGRG | 4 cut(s) | 527, 720, 1593, 2184 |
| AvaII | GGWCC | 3 cut(s) | 1598, 1671, 2159 |
| BaeGI | GKGCMC | 1 cut(s) | 646 |
| BalI | TGGCCA | 1 cut(s) | 1019 |
| BanI | GGYRCC | 1 cut(s) | 1391 |
| BanII | GRGCYC | 3 cut(s) | 1805, 1959, 2118 |
| BbsI | GAAGAC | 2 cut(s) | 30, 1266 |
| Bbv12I | GWGCWC | 3 cut(s) | 646, 829, 2118 |
| BbvCI | CCTCAGC | 1 cut(s) | 1875 |
| BbvI | GCAGC | 9 cut(s) | 310, 773, 863, 886, 891, 1121, 1348, 1627, 1820 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1247, 1281, 1622, 1656 |
| BciT130I | CCWGG | 4 cut(s) | 96, 1151, 1647, 1953 |
| BciVI | GTATCC | 1 cut(s) | 2349 |
| BcnI | CCSGG | 2 cut(s) | 369, 1270 |
| BcoDI | GTCTC | 5 cut(s) | 234, 537, 555, 1403, 1615 |
| BfaI | CTAG | 3 cut(s) | 417, 950, 2046 |
| BfmI | CTRYAG | 6 cut(s) | 131, 324, 759, 877, 1167, 1806 |
| BfoI | RGCGCY | 1 cut(s) | 1884 |
| BfuAI | ACCTGC | 2 cut(s) | 2100, 2206 |
| BfuI | GTATCC | 1 cut(s) | 2349 |
| BlpI | GCTNAGC | 1 cut(s) | 883 |
| Bme1390I | CCNGG | 6 cut(s) | 96, 369, 1151, 1270, 1647, 1953 |
| Bme18I | GGWCC | 3 cut(s) | 1598, 1671, 2159 |
| BmeT110I | CYCGRG | 4 cut(s) | 527, 720, 1593, 2184 |
| BmgBI | CACGTC | 1 cut(s) | 934 |
| BmgT120I | GGNCC | 5 cut(s) | 525, 1058, 1598, 1671, 2159 |
| BmiI | GGNNCC | 6 cut(s) | 87, 146, 1393, 1600, 1673, 1802 |
| BmrFI | CCNGG | 6 cut(s) | 96, 369, 1151, 1270, 1647, 1953 |
| BmrI | ACTGGG | 1 cut(s) | 2338 |
| BmsI | GCATC | 7 cut(s) | 715, 901, 953, 1240, 1250, 1794, 2058 |
| BmtI | GCTAGC | 1 cut(s) | 2049 |
| BmuI | ACTGGG | 1 cut(s) | 2338 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2072 |
| BpiI | GAAGAC | 2 cut(s) | 30, 1266 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2344, 2376 |
| BpmI | CTGGAG | 3 cut(s) | 78, 1692, 2282 |
| Bpu10I | CCTNAGC | 2 cut(s) | 213, 1875 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 883 |
| BpuEI | CTTGAG | 2 cut(s) | 327, 2102 |
| BpuMI | CCSGG | 2 cut(s) | 369, 1270 |
| BsaI | GGTCTC | 1 cut(s) | 1403 |
| BsaJI | CCNNGG | 9 cut(s) | 95, 1091, 1149, 1150, 1440, 1740, 1951, 1952, 2004 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 58, 95, 217, 1576 |
| Bse118I | RCCGGY | 3 cut(s) | 764, 1394, 2201 |
| Bse1I | ACTGG | 5 cut(s) | 520, 874, 1709, 2162, 2333 |
| Bse3DI | GCAATG | 3 cut(s) | 619, 1236, 2178 |
| BseBI | CCWGG | 4 cut(s) | 96, 1151, 1647, 1953 |
| BseDI | CCNNGG | 9 cut(s) | 95, 1091, 1149, 1150, 1440, 1740, 1951, 1952, 2004 |
| BseGI | GGATG | 9 cut(s) | 752, 991, 1200, 1204, 1352, 1432, 1785, 1986, 2133 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 58, 95, 217, 1576 |
| BseMI | GCAATG | 3 cut(s) | 619, 1236, 2178 |
| BseMII | CTCAG | 7 cut(s) | 204, 216, 897, 1424, 1866, 1901, 1992 |
| BseNI | ACTGG | 5 cut(s) | 520, 874, 1709, 2162, 2333 |
| BseRI | GAGGAG | 4 cut(s) | 78, 81, 834, 1875 |
| BseSI | GKGCMC | 1 cut(s) | 646 |
| BseXI | GCAGC | 9 cut(s) | 310, 773, 863, 886, 891, 1121, 1348, 1627, 1820 |
| BseYI | CCCAGC | 2 cut(s) | 1385, 1418 |
| BsgI | GTGCAG | 1 cut(s) | 1347 |
| BshFI | GGCC | 4 cut(s) | 51, 526, 1019, 1059 |
| BshNI | GGYRCC | 1 cut(s) | 1391 |
| BsiHKAI | GWGCWC | 3 cut(s) | 646, 829, 2118 |
| BsiHKCI | CYCGRG | 4 cut(s) | 527, 720, 1593, 2184 |
| BsiSI | CCGG | 5 cut(s) | 369, 765, 1269, 1395, 2202 |
| BslFI | GGGAC | 3 cut(s) | 810, 1657, 2268 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 58, 95, 217, 1576 |
| BsmAI | GTCTC | 5 cut(s) | 234, 537, 555, 1403, 1615 |
| BsmFI | GGGAC | 3 cut(s) | 810, 1657, 2268 |
| BsmI | GAATGC | 1 cut(s) | 1580 |
| BsnI | GGCC | 4 cut(s) | 51, 526, 1019, 1059 |
| Bso31I | GGTCTC | 1 cut(s) | 1403 |
| BsoBI | CYCGRG | 4 cut(s) | 527, 720, 1593, 2184 |
| Bsp1286I | GDGCHC | 5 cut(s) | 646, 829, 1805, 1959, 2118 |
| Bsp1407I | TGTACA | 1 cut(s) | 291 |
| Bsp143I | GATC | 3 cut(s) | 1349, 1849, 1900 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 883 |
| Bsp19I | CCATGG | 2 cut(s) | 1740, 2004 |
| BspACI | CCGC | 3 cut(s) | 165, 273, 959 |
| BspANI | GGCC | 4 cut(s) | 51, 526, 1019, 1059 |
| BspCNI | CTCAG | 7 cut(s) | 205, 217, 896, 1423, 1867, 1900, 1991 |
| BspHI | TCATGA | 2 cut(s) | 235, 2110 |
| BspLI | GGNNCC | 6 cut(s) | 87, 146, 1393, 1600, 1673, 1802 |
| BspMAI | CTGCAG | 5 cut(s) | 135, 328, 763, 881, 1810 |
| BspMI | ACCTGC | 2 cut(s) | 2100, 2206 |
| BspOI | GCTAGC | 1 cut(s) | 2049 |
| BspPI | GGATC | 1 cut(s) | 1895 |
| BspQI | GCTCTTC | 1 cut(s) | 1779 |
| BspT107I | GGYRCC | 1 cut(s) | 1391 |
| BspTNI | GGTCTC | 1 cut(s) | 1403 |
| BsrDI | GCAATG | 3 cut(s) | 619, 1236, 2178 |
| BsrFI | RCCGGY | 3 cut(s) | 764, 1394, 2201 |
| BsrGI | TGTACA | 1 cut(s) | 291 |
| BsrI | ACTGG | 5 cut(s) | 520, 874, 1709, 2162, 2333 |
| BssAI | RCCGGY | 3 cut(s) | 764, 1394, 2201 |
| BssECI | CCNNGG | 9 cut(s) | 95, 1091, 1149, 1150, 1440, 1740, 1951, 1952, 2004 |
| BssMI | GATC | 3 cut(s) | 1349, 1849, 1900 |
| BssNAI | GTATAC | 1 cut(s) | 1188 |
| BssT1I | CCWWGG | 3 cut(s) | 1091, 1740, 2004 |
| Bst1107I | GTATAC | 1 cut(s) | 1188 |
| Bst2UI | CCWGG | 4 cut(s) | 96, 1151, 1647, 1953 |
| Bst6I | CTCTTC | 1 cut(s) | 1779 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2055 |
| BstAUI | TGTACA | 1 cut(s) | 291 |
| BstC8I | GCNNGC | 5 cut(s) | 646, 787, 1114, 2047, 2203 |
| BstDEI | CTNAG | 7 cut(s) | 213, 225, 883, 1410, 1875, 1887, 1978 |
| BstDSI | CCRYGG | 2 cut(s) | 1740, 2004 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 93 |
| BstF5I | GGATG | 9 cut(s) | 752, 991, 1200, 1204, 1352, 1432, 1785, 1986, 2133 |
| BstH2I | RGCGCY | 1 cut(s) | 1884 |
| BstHHI | GCGC | 2 cut(s) | 1883, 2033 |
| BstKTI | GATC | 3 cut(s) | 1352, 1852, 1903 |
| BstMAI | GTCTC | 5 cut(s) | 234, 537, 555, 1403, 1615 |
| BstMBI | GATC | 3 cut(s) | 1349, 1849, 1900 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 24, 1391, 1880, 2030, 2055 |
| BstNI | CCWGG | 4 cut(s) | 96, 1151, 1647, 1953 |
| BstNSI | RCATGY | 3 cut(s) | 31, 1928, 2058 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2072 |
| BstSCI | CCNGG | 6 cut(s) | 94, 367, 1149, 1268, 1645, 1951 |
| BstSFI | CTRYAG | 6 cut(s) | 131, 324, 759, 877, 1167, 1806 |
| BstSLI | GKGCMC | 1 cut(s) | 646 |
| BstV1I | GCAGC | 9 cut(s) | 310, 773, 863, 886, 891, 1121, 1348, 1627, 1820 |
| BstV2I | GAAGAC | 2 cut(s) | 30, 1266 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 576, 837 |
| BstZ17I | GTATAC | 1 cut(s) | 1188 |
| BsuI | GTATCC | 1 cut(s) | 2349 |
| BsuRI | GGCC | 4 cut(s) | 51, 526, 1019, 1059 |
| BtgI | CCRYGG | 2 cut(s) | 1740, 2004 |
| BtrI | CACGTC | 1 cut(s) | 934 |
| BtsCI | GGATG | 9 cut(s) | 752, 991, 1200, 1204, 1352, 1432, 1785, 1986, 2133 |
| BtsI | GCAGTG | 2 cut(s) | 756, 1356 |
| BtsIMutI | CAGTG | 6 cut(s) | 513, 756, 1101, 1270, 1356, 2373 |
| BveI | ACCTGC | 2 cut(s) | 2100, 2206 |
| Cac8I | GCNNGC | 5 cut(s) | 646, 787, 1114, 2047, 2203 |
| CciI | TCATGA | 2 cut(s) | 235, 2110 |
| CfoI | GCGC | 2 cut(s) | 1883, 2033 |
| Cfr10I | RCCGGY | 3 cut(s) | 764, 1394, 2201 |
| Cfr13I | GGNCC | 5 cut(s) | 525, 1058, 1598, 1671, 2159 |
| Csp6I | GTAC | 6 cut(s) | 292, 365, 741, 1299, 1622, 2326 |
| CviQI | GTAC | 6 cut(s) | 292, 365, 741, 1299, 1622, 2326 |
| DdeI | CTNAG | 7 cut(s) | 213, 225, 883, 1410, 1875, 1887, 1978 |
| DpnI | GATC | 3 cut(s) | 1351, 1851, 1902 |
| DpnII | GATC | 3 cut(s) | 1349, 1849, 1900 |
| DraI | TTTAAA | 1 cut(s) | 1720 |
| EaeI | YGGCCR | 1 cut(s) | 1017 |
| Eam1104I | CTCTTC | 1 cut(s) | 1779 |
| EarI | CTCTTC | 1 cut(s) | 1779 |
| EciI | GGCGGA | 1 cut(s) | 948 |
| Ecl136II | GAGCTC | 1 cut(s) | 2116 |
| Eco130I | CCWWGG | 3 cut(s) | 1091, 1740, 2004 |
| Eco24I | GRGCYC | 3 cut(s) | 1805, 1959, 2118 |
| Eco31I | GGTCTC | 1 cut(s) | 1403 |
| Eco32I | GATATC | 1 cut(s) | 1566 |
| Eco47I | GGWCC | 3 cut(s) | 1598, 1671, 2159 |
| Eco53kI | GAGCTC | 1 cut(s) | 2116 |
| Eco57I | CTGAAG | 4 cut(s) | 77, 714, 789, 1646 |
| Eco88I | CYCGRG | 4 cut(s) | 527, 720, 1593, 2184 |
| EcoICRI | GAGCTC | 1 cut(s) | 2116 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 93 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1598 |
| EcoRII | CCWGG | 4 cut(s) | 94, 1149, 1645, 1951 |
| EcoRV | GATATC | 1 cut(s) | 1566 |
| EcoT14I | CCWWGG | 3 cut(s) | 1091, 1740, 2004 |
| EcoT38I | GRGCYC | 3 cut(s) | 1805, 1959, 2118 |
| ErhI | CCWWGG | 3 cut(s) | 1091, 1740, 2004 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2296, 2328 |
| FaqI | GGGAC | 3 cut(s) | 810, 1657, 2268 |
| FauNDI | CATATG | 3 cut(s) | 81, 250, 587 |
| FblI | GTMKAC | 3 cut(s) | 441, 1187, 1450 |
| FokI | GGATG | 9 cut(s) | 739, 998, 1207, 1211, 1339, 1439, 1772, 1973, 2140 |
| FriOI | GRGCYC | 3 cut(s) | 1805, 1959, 2118 |
| FspBI | CTAG | 3 cut(s) | 417, 950, 2046 |
| GlaI | GCGC | 2 cut(s) | 1882, 2032 |
| GsaI | CCCAGC | 2 cut(s) | 1389, 1422 |
| GsuI | CTGGAG | 3 cut(s) | 78, 1692, 2282 |
| HaeII | RGCGCY | 1 cut(s) | 1884 |
| HaeIII | GGCC | 4 cut(s) | 51, 526, 1019, 1059 |
| HapII | CCGG | 5 cut(s) | 369, 765, 1269, 1395, 2202 |
| HhaI | GCGC | 2 cut(s) | 1883, 2033 |
| Hin6I | GCGC | 2 cut(s) | 1881, 2031 |
| HinP1I | GCGC | 2 cut(s) | 1881, 2031 |
| HindIII | AAGCTT | 2 cut(s) | 538, 787 |
| HinfI | GANTC | 7 cut(s) | 227, 241, 281, 443, 504, 530, 1727 |
| HpaII | CCGG | 5 cut(s) | 369, 765, 1269, 1395, 2202 |
| HphI | GGTGA | 2 cut(s) | 100, 2095 |
| Hpy188I | TCNGA | 4 cut(s) | 226, 1444, 1890, 1981 |
| Hpy188III | TCNNGA | 8 cut(s) | 236, 401, 593, 732, 807, 1731, 2111, 2139 |
| HpyAV | CCTTC | 5 cut(s) | 52, 158, 590, 1626, 2106 |
| HpyCH4IV | ACGT | 1 cut(s) | 933 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 24, 1391, 1880, 2030, 2055 |
| HpyF3I | CTNAG | 7 cut(s) | 213, 225, 883, 1410, 1875, 1887, 1978 |
| HpySE526I | ACGT | 1 cut(s) | 933 |
| HspAI | GCGC | 2 cut(s) | 1881, 2031 |
| KroI | GCCGGC | 1 cut(s) | 2201 |
| KroNI | GCCGGC | 1 cut(s) | 2203 |
| Kzo9I | GATC | 3 cut(s) | 1349, 1849, 1900 |
| LguI | GCTCTTC | 1 cut(s) | 1779 |
| Lsp1109I | GCAGC | 9 cut(s) | 310, 773, 863, 886, 891, 1121, 1348, 1627, 1820 |
| LweI | GCATC | 7 cut(s) | 715, 901, 953, 1240, 1250, 1794, 2058 |
| MaeI | CTAG | 3 cut(s) | 417, 950, 2046 |
| MaeII | ACGT | 1 cut(s) | 933 |
| MaeIII | GTNAC | 2 cut(s) | 106, 2146 |
| MalI | GATC | 3 cut(s) | 1351, 1851, 1902 |
| MboI | GATC | 3 cut(s) | 1349, 1849, 1900 |
| MfeI | CAATTG | 1 cut(s) | 1476 |
| MhlI | GDGCHC | 5 cut(s) | 646, 829, 1805, 1959, 2118 |
| MlsI | TGGCCA | 1 cut(s) | 1019 |
| MluNI | TGGCCA | 1 cut(s) | 1019 |
| MlyI | GAGTC | 4 cut(s) | 236, 437, 513, 539 |
| MmeI | TCCRAC | 2 cut(s) | 404, 557 |
| Mox20I | TGGCCA | 1 cut(s) | 1019 |
| MroNI | GCCGGC | 1 cut(s) | 2201 |
| MroXI | GAANNNNTTC | 3 cut(s) | 285, 1208, 2308 |
| MscI | TGGCCA | 1 cut(s) | 1019 |
| MseI | TTAA | 8 cut(s) | 657, 1532, 1719, 1779, 2082, 2166, 2214, 2220 |
| MslI | CAYNNNNRTG | 1 cut(s) | 80 |
| Msp20I | TGGCCA | 1 cut(s) | 1019 |
| MspI | CCGG | 5 cut(s) | 369, 765, 1269, 1395, 2202 |
| MspR9I | CCNGG | 6 cut(s) | 96, 369, 1151, 1270, 1647, 1953 |
| MunI | CAATTG | 1 cut(s) | 1476 |
| Mva1269I | GAATGC | 1 cut(s) | 1580 |
| MvaI | CCWGG | 4 cut(s) | 96, 1151, 1647, 1953 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 24, 1391, 1880, 2030, 2055 |
| NaeI | GCCGGC | 1 cut(s) | 2203 |
| NciI | CCSGG | 2 cut(s) | 369, 1270 |
| NcoI | CCATGG | 2 cut(s) | 1740, 2004 |
| NdeI | CATATG | 3 cut(s) | 81, 250, 587 |
| NdeII | GATC | 3 cut(s) | 1349, 1849, 1900 |
| NgoMIV | GCCGGC | 1 cut(s) | 2201 |
| NheI | GCTAGC | 1 cut(s) | 2045 |
| NlaIV | GGNNCC | 6 cut(s) | 87, 146, 1393, 1600, 1673, 1802 |
| NmuCI | GTSAC | 2 cut(s) | 106, 2146 |
| NspI | RCATGY | 3 cut(s) | 31, 1928, 2058 |
| PaeR7I | CTCGAG | 1 cut(s) | 1593 |
| PagI | TCATGA | 2 cut(s) | 235, 2110 |
| PaqCI | CACCTGC | 1 cut(s) | 2206 |
| PasI | CCCWGGG | 2 cut(s) | 1150, 1952 |
| PciI | ACATGT | 1 cut(s) | 1924 |
| PciSI | GCTCTTC | 1 cut(s) | 1779 |
| PctI | GAATGC | 1 cut(s) | 1580 |
| PdiI | GCCGGC | 1 cut(s) | 2203 |
| PdmI | GAANNNNTTC | 3 cut(s) | 285, 1208, 2308 |
| PfeI | GAWTC | 3 cut(s) | 241, 281, 1727 |
| PleI | GAGTC | 4 cut(s) | 235, 437, 512, 538 |
| PpsI | GAGTC | 4 cut(s) | 235, 437, 512, 538 |
| PpuMI | RGGWCCY | 1 cut(s) | 1598 |
| PscI | ACATGT | 1 cut(s) | 1924 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2072 |
| Psp124BI | GAGCTC | 1 cut(s) | 2118 |
| Psp5II | RGGWCCY | 1 cut(s) | 1598 |
| Psp6I | CCWGG | 4 cut(s) | 94, 1149, 1645, 1951 |
| PspFI | CCCAGC | 2 cut(s) | 1385, 1418 |
| PspGI | CCWGG | 4 cut(s) | 94, 1149, 1645, 1951 |
| PspN4I | GGNNCC | 6 cut(s) | 87, 146, 1393, 1600, 1673, 1802 |
| PspPI | GGNCC | 5 cut(s) | 525, 1058, 1598, 1671, 2159 |
| PspPPI | RGGWCCY | 1 cut(s) | 1598 |
| PspXI | VCTCGAGB | 1 cut(s) | 1593 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1196, 1228 |
| PstI | CTGCAG | 5 cut(s) | 135, 328, 763, 881, 1810 |
| RsaI | GTAC | 6 cut(s) | 293, 366, 742, 1300, 1623, 2327 |
| RsaNI | GTAC | 6 cut(s) | 292, 365, 741, 1299, 1622, 2326 |
| RseI | CAYNNNNRTG | 1 cut(s) | 80 |
| SacI | GAGCTC | 1 cut(s) | 2118 |
| SapI | GCTCTTC | 1 cut(s) | 1779 |
| SaqAI | TTAA | 8 cut(s) | 657, 1532, 1719, 1779, 2082, 2166, 2214, 2220 |
| Sau3AI | GATC | 3 cut(s) | 1349, 1849, 1900 |
| Sau96I | GGNCC | 5 cut(s) | 525, 1058, 1598, 1671, 2159 |
| SchI | GAGTC | 4 cut(s) | 236, 437, 513, 539 |
| ScrFI | CCNGG | 6 cut(s) | 96, 369, 1151, 1270, 1647, 1953 |
| SduI | GDGCHC | 5 cut(s) | 646, 829, 1805, 1959, 2118 |
| SfaNI | GCATC | 7 cut(s) | 715, 901, 953, 1240, 1250, 1794, 2058 |
| SfcI | CTRYAG | 6 cut(s) | 131, 324, 759, 877, 1167, 1806 |
| Sfr274I | CTCGAG | 1 cut(s) | 1593 |
| SinI | GGWCC | 3 cut(s) | 1598, 1671, 2159 |
| SlaI | CTCGAG | 1 cut(s) | 1593 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 80 |
| SmlI | CTYRAG | 3 cut(s) | 342, 1593, 2117 |
| SmoI | CTYRAG | 3 cut(s) | 342, 1593, 2117 |
| SsiI | CCGC | 3 cut(s) | 165, 273, 959 |
| SspMI | CTAG | 3 cut(s) | 417, 950, 2046 |
| SstI | GAGCTC | 1 cut(s) | 2118 |
| StyD4I | CCNGG | 6 cut(s) | 94, 367, 1149, 1268, 1645, 1951 |
| StyI | CCWWGG | 3 cut(s) | 1091, 1740, 2004 |
| TaiI | ACGT | 1 cut(s) | 936 |
| TaqI | TCGA | 5 cut(s) | 1122, 1594, 1869, 1908, 2066 |
| TatI | WGTACW | 2 cut(s) | 291, 2325 |
| TauI | GCSGC | 1 cut(s) | 275 |
| TfiI | GAWTC | 3 cut(s) | 241, 281, 1727 |
| Tru1I | TTAA | 8 cut(s) | 657, 1532, 1719, 1779, 2082, 2166, 2214, 2220 |
| Tru9I | TTAA | 8 cut(s) | 657, 1532, 1719, 1779, 2082, 2166, 2214, 2220 |
| TscAI | CASTG | 6 cut(s) | 520, 763, 1108, 1270, 1363, 2373 |
| TseFI | GTSAC | 2 cut(s) | 106, 2146 |
| TseI | GCWGC | 9 cut(s) | 323, 761, 876, 879, 899, 1109, 1361, 1640, 1808 |
| Tsp45I | GTSAC | 2 cut(s) | 106, 2146 |
| TspDTI | ATGAA | 5 cut(s) | 17, 681, 998, 2099, 2333 |
| TspGWI | ACGGA | 1 cut(s) | 1979 |
| TspRI | CASTG | 6 cut(s) | 520, 763, 1108, 1270, 1363, 2373 |
| VneI | GTGCAC | 1 cut(s) | 642 |
| VpaK11BI | GGWCC | 3 cut(s) | 1598, 1671, 2159 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 93 |
| XapI | RAATTY | 6 cut(s) | 519, 674, 707, 1026, 1081, 1316 |
| XceI | RCATGY | 3 cut(s) | 31, 1928, 2058 |
| XhoI | CTCGAG | 1 cut(s) | 1593 |
| XmiI | GTMKAC | 3 cut(s) | 441, 1187, 1450 |
| XmnI | GAANNNNTTC | 3 cut(s) | 285, 1208, 2308 |
| XspI | CTAG | 3 cut(s) | 417, 950, 2046 |