Subtilase family
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_2g10210 FvH4_2g10210 FvH4_2g10210 |
| malus_domestica | MD05G1055900.v1.1 |
| prunus_persica | Prupe.8G086300_v2.0.a1 |
| pyrus_communis | pycom05g04760 |
| rosa_chinensis | RchiOBHm_Chr6g0267441 |
| rosa_laevigata | RLG00000014004 |
| rosa_multiflora | Rmu_sc0001068.1_g000015 Rmu_sc0006674.1_g000011 |
| rosa_roxburghii | Rroxscaffold_7G00200780 |
| rosa_rugosa | Rorug06G0032600 Rorug06G0032600 |
| rosa_samantha | Rh6AG154000 Rh6BG154100 Rh6CG149800 |
| rosa_wichuraiana | Rw6G013220 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2209 |
| Acc36I | ACCTGC | 2 cut(s) | 2103, 2209 |
| AccB1I | GGYRCC | 1 cut(s) | 1394 |
| AccI | GTMKAC | 3 cut(s) | 444, 1190, 1453 |
| AccII | CGCG | 1 cut(s) | 2190 |
| AciI | CCGC | 1 cut(s) | 276 |
| AclWI | GGATC | 1 cut(s) | 1898 |
| AcoI | YGGCCR | 1 cut(s) | 1020 |
| AcsI | RAATTY | 5 cut(s) | 522, 677, 710, 1084, 1319 |
| AcuI | CTGAAG | 4 cut(s) | 77, 717, 792, 1649 |
| AfaI | GTAC | 6 cut(s) | 296, 369, 745, 1303, 1626, 2330 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 58, 98, 220, 1579 |
| AflIII | ACRYGT | 1 cut(s) | 1927 |
| AgsI | TTSAA | 9 cut(s) | 42, 474, 613, 710, 1466, 1730, 2195, 2245, 2272 |
| AjiI | CACGTC | 1 cut(s) | 937 |
| AjnI | CCWGG | 3 cut(s) | 97, 1152, 1648 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 663, 695 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1308, 1340 |
| Alw21I | GWGCWC | 3 cut(s) | 649, 832, 2121 |
| Alw26I | GTCTC | 5 cut(s) | 237, 540, 558, 1406, 1618 |
| Alw44I | GTGCAC | 1 cut(s) | 645 |
| AlwI | GGATC | 1 cut(s) | 1898 |
| Ama87I | CYCGRG | 4 cut(s) | 530, 723, 1596, 1955 |
| AoxI | GGCC | 4 cut(s) | 49, 527, 1020, 1060 |
| ApaLI | GTGCAC | 1 cut(s) | 645 |
| ApeKI | GCWGC | 9 cut(s) | 326, 764, 879, 882, 902, 1112, 1364, 1643, 1811 |
| ApoI | RAATTY | 5 cut(s) | 522, 677, 710, 1084, 1319 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 288, 1211, 2311 |
| AspLEI | GCGC | 2 cut(s) | 1886, 2036 |
| AspS9I | GGNCC | 5 cut(s) | 528, 1061, 1601, 1674, 2162 |
| AsuC2I | CCSGG | 4 cut(s) | 372, 1273, 1956, 1957 |
| AsuHPI | GGTGA | 3 cut(s) | 103, 227, 2098 |
| AsuNHI | GCTAGC | 1 cut(s) | 2048 |
| AvaI | CYCGRG | 4 cut(s) | 530, 723, 1596, 1955 |
| AvaII | GGWCC | 3 cut(s) | 1601, 1674, 2162 |
| BaeGI | GKGCMC | 1 cut(s) | 649 |
| BalI | TGGCCA | 1 cut(s) | 1022 |
| BanI | GGYRCC | 1 cut(s) | 1394 |
| BanII | GRGCYC | 3 cut(s) | 1808, 1962, 2121 |
| BbsI | GAAGAC | 2 cut(s) | 30, 1269 |
| Bbv12I | GWGCWC | 3 cut(s) | 649, 832, 2121 |
| BbvCI | CCTCAGC | 1 cut(s) | 1878 |
| BbvI | GCAGC | 9 cut(s) | 313, 751, 866, 889, 894, 1124, 1351, 1630, 1823 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1250, 1284, 1625, 1659 |
| BciT130I | CCWGG | 3 cut(s) | 99, 1154, 1650 |
| BciVI | GTATCC | 1 cut(s) | 2352 |
| BcnI | CCSGG | 4 cut(s) | 372, 1273, 1956, 1957 |
| BcoDI | GTCTC | 5 cut(s) | 237, 540, 558, 1406, 1618 |
| BfaI | CTAG | 3 cut(s) | 420, 953, 2049 |
| BfmI | CTRYAG | 5 cut(s) | 134, 327, 880, 1170, 1809 |
| BfoI | RGCGCY | 1 cut(s) | 1887 |
| BfuAI | ACCTGC | 2 cut(s) | 2103, 2209 |
| BfuI | GTATCC | 1 cut(s) | 2352 |
| BlpI | GCTNAGC | 1 cut(s) | 886 |
| Bme1390I | CCNGG | 7 cut(s) | 99, 372, 1154, 1273, 1650, 1956, 1957 |
| Bme18I | GGWCC | 3 cut(s) | 1601, 1674, 2162 |
| BmeT110I | CYCGRG | 4 cut(s) | 530, 723, 1596, 1955 |
| BmgBI | CACGTC | 1 cut(s) | 937 |
| BmgT120I | GGNCC | 5 cut(s) | 528, 1061, 1601, 1674, 2162 |
| BmiI | GGNNCC | 6 cut(s) | 87, 149, 1396, 1603, 1676, 1805 |
| BmrFI | CCNGG | 7 cut(s) | 99, 372, 1154, 1273, 1650, 1956, 1957 |
| BmrI | ACTGGG | 1 cut(s) | 2341 |
| BmsI | GCATC | 7 cut(s) | 718, 904, 956, 1243, 1253, 1797, 2061 |
| BmtI | GCTAGC | 1 cut(s) | 2052 |
| BmuI | ACTGGG | 1 cut(s) | 2341 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2075 |
| BpiI | GAAGAC | 2 cut(s) | 30, 1269 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2347, 2379 |
| BpmI | CTGGAG | 3 cut(s) | 81, 1695, 2285 |
| Bpu10I | CCTNAGC | 2 cut(s) | 216, 1878 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 886 |
| BpuEI | CTTGAG | 2 cut(s) | 330, 2105 |
| BpuMI | CCSGG | 4 cut(s) | 372, 1273, 1956, 1957 |
| BsaI | GGTCTC | 1 cut(s) | 1406 |
| BsaJI | CCNNGG | 9 cut(s) | 98, 1094, 1152, 1153, 1443, 1743, 1954, 1955, 2007 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 58, 98, 220, 1579 |
| Bse118I | RCCGGY | 3 cut(s) | 767, 1397, 2204 |
| Bse1I | ACTGG | 5 cut(s) | 523, 877, 1712, 2165, 2336 |
| Bse3DI | GCAATG | 3 cut(s) | 622, 1239, 2181 |
| BseBI | CCWGG | 3 cut(s) | 99, 1154, 1650 |
| BseDI | CCNNGG | 9 cut(s) | 98, 1094, 1152, 1153, 1443, 1743, 1954, 1955, 2007 |
| BseGI | GGATG | 9 cut(s) | 755, 994, 1203, 1207, 1355, 1435, 1788, 1989, 2136 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 58, 98, 220, 1579 |
| BseMI | GCAATG | 3 cut(s) | 622, 1239, 2181 |
| BseMII | CTCAG | 8 cut(s) | 207, 219, 900, 1047, 1427, 1869, 1904, 1995 |
| BseNI | ACTGG | 5 cut(s) | 523, 877, 1712, 2165, 2336 |
| BseRI | GAGGAG | 5 cut(s) | 78, 81, 84, 837, 1878 |
| BseSI | GKGCMC | 1 cut(s) | 649 |
| BseXI | GCAGC | 9 cut(s) | 313, 751, 866, 889, 894, 1124, 1351, 1630, 1823 |
| BseYI | CCCAGC | 2 cut(s) | 1388, 1421 |
| BsgI | GTGCAG | 1 cut(s) | 1350 |
| Bsh1236I | CGCG | 1 cut(s) | 2190 |
| BshFI | GGCC | 4 cut(s) | 51, 529, 1022, 1062 |
| BshNI | GGYRCC | 1 cut(s) | 1394 |
| BsiHKAI | GWGCWC | 3 cut(s) | 649, 832, 2121 |
| BsiHKCI | CYCGRG | 4 cut(s) | 530, 723, 1596, 1955 |
| BsiSI | CCGG | 6 cut(s) | 372, 768, 1272, 1398, 1956, 2205 |
| BslFI | GGGAC | 3 cut(s) | 813, 1660, 2271 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 58, 98, 220, 1579 |
| BsmAI | GTCTC | 5 cut(s) | 237, 540, 558, 1406, 1618 |
| BsmFI | GGGAC | 3 cut(s) | 813, 1660, 2271 |
| BsmI | GAATGC | 1 cut(s) | 1583 |
| BsnI | GGCC | 4 cut(s) | 51, 529, 1022, 1062 |
| Bso31I | GGTCTC | 1 cut(s) | 1406 |
| BsoBI | CYCGRG | 4 cut(s) | 530, 723, 1596, 1955 |
| Bsp1286I | GDGCHC | 5 cut(s) | 649, 832, 1808, 1962, 2121 |
| Bsp1407I | TGTACA | 1 cut(s) | 294 |
| Bsp143I | GATC | 2 cut(s) | 1852, 1903 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 886 |
| Bsp19I | CCATGG | 2 cut(s) | 1743, 2007 |
| BspACI | CCGC | 1 cut(s) | 276 |
| BspANI | GGCC | 4 cut(s) | 51, 529, 1022, 1062 |
| BspCNI | CTCAG | 8 cut(s) | 208, 220, 899, 1046, 1426, 1870, 1903, 1994 |
| BspFNI | CGCG | 1 cut(s) | 2190 |
| BspHI | TCATGA | 1 cut(s) | 2113 |
| BspLI | GGNNCC | 6 cut(s) | 87, 149, 1396, 1603, 1676, 1805 |
| BspMAI | CTGCAG | 4 cut(s) | 138, 331, 884, 1813 |
| BspMI | ACCTGC | 2 cut(s) | 2103, 2209 |
| BspOI | GCTAGC | 1 cut(s) | 2052 |
| BspPI | GGATC | 1 cut(s) | 1898 |
| BspQI | GCTCTTC | 1 cut(s) | 1782 |
| BspT107I | GGYRCC | 1 cut(s) | 1394 |
| BspTNI | GGTCTC | 1 cut(s) | 1406 |
| BsrDI | GCAATG | 3 cut(s) | 622, 1239, 2181 |
| BsrFI | RCCGGY | 3 cut(s) | 767, 1397, 2204 |
| BsrGI | TGTACA | 1 cut(s) | 294 |
| BsrI | ACTGG | 5 cut(s) | 523, 877, 1712, 2165, 2336 |
| BssAI | RCCGGY | 3 cut(s) | 767, 1397, 2204 |
| BssECI | CCNNGG | 9 cut(s) | 98, 1094, 1152, 1153, 1443, 1743, 1954, 1955, 2007 |
| BssMI | GATC | 2 cut(s) | 1852, 1903 |
| BssNAI | GTATAC | 1 cut(s) | 1191 |
| BssT1I | CCWWGG | 3 cut(s) | 1094, 1743, 2007 |
| Bst1107I | GTATAC | 1 cut(s) | 1191 |
| Bst2UI | CCWGG | 3 cut(s) | 99, 1154, 1650 |
| Bst6I | CTCTTC | 1 cut(s) | 1782 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2058 |
| BstAUI | TGTACA | 1 cut(s) | 294 |
| BstC8I | GCNNGC | 6 cut(s) | 649, 790, 1117, 2050, 2188, 2206 |
| BstDEI | CTNAG | 8 cut(s) | 216, 228, 886, 1033, 1413, 1878, 1890, 1981 |
| BstDSI | CCRYGG | 2 cut(s) | 1743, 2007 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 96 |
| BstF5I | GGATG | 9 cut(s) | 755, 994, 1203, 1207, 1355, 1435, 1788, 1989, 2136 |
| BstFNI | CGCG | 1 cut(s) | 2190 |
| BstH2I | RGCGCY | 1 cut(s) | 1887 |
| BstHHI | GCGC | 2 cut(s) | 1886, 2036 |
| BstKTI | GATC | 2 cut(s) | 1855, 1906 |
| BstMAI | GTCTC | 5 cut(s) | 237, 540, 558, 1406, 1618 |
| BstMBI | GATC | 2 cut(s) | 1852, 1903 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 24, 1394, 1883, 2033, 2058 |
| BstNI | CCWGG | 3 cut(s) | 99, 1154, 1650 |
| BstNSI | RCATGY | 3 cut(s) | 31, 1931, 2061 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2075 |
| BstSCI | CCNGG | 7 cut(s) | 97, 370, 1152, 1271, 1648, 1954, 1955 |
| BstSFI | CTRYAG | 5 cut(s) | 134, 327, 880, 1170, 1809 |
| BstSLI | GKGCMC | 1 cut(s) | 649 |
| BstUI | CGCG | 1 cut(s) | 2190 |
| BstV1I | GCAGC | 9 cut(s) | 313, 751, 866, 889, 894, 1124, 1351, 1630, 1823 |
| BstV2I | GAAGAC | 2 cut(s) | 30, 1269 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 579, 840 |
| BstZ17I | GTATAC | 1 cut(s) | 1191 |
| BsuI | GTATCC | 1 cut(s) | 2352 |
| BsuRI | GGCC | 4 cut(s) | 51, 529, 1022, 1062 |
| BtgI | CCRYGG | 2 cut(s) | 1743, 2007 |
| BtrI | CACGTC | 1 cut(s) | 937 |
| BtsCI | GGATG | 9 cut(s) | 755, 994, 1203, 1207, 1355, 1435, 1788, 1989, 2136 |
| BtsI | GCAGTG | 2 cut(s) | 759, 1359 |
| BtsIMutI | CAGTG | 6 cut(s) | 516, 759, 1104, 1273, 1359, 2376 |
| BveI | ACCTGC | 2 cut(s) | 2103, 2209 |
| Cac8I | GCNNGC | 6 cut(s) | 649, 790, 1117, 2050, 2188, 2206 |
| CciI | TCATGA | 1 cut(s) | 2113 |
| CfoI | GCGC | 2 cut(s) | 1886, 2036 |
| Cfr10I | RCCGGY | 3 cut(s) | 767, 1397, 2204 |
| Cfr13I | GGNCC | 5 cut(s) | 528, 1061, 1601, 1674, 2162 |
| Cfr9I | CCCGGG | 1 cut(s) | 1955 |
| Csp6I | GTAC | 6 cut(s) | 295, 368, 744, 1302, 1625, 2329 |
| CviQI | GTAC | 6 cut(s) | 295, 368, 744, 1302, 1625, 2329 |
| DdeI | CTNAG | 8 cut(s) | 216, 228, 886, 1033, 1413, 1878, 1890, 1981 |
| DpnI | GATC | 2 cut(s) | 1854, 1905 |
| DpnII | GATC | 2 cut(s) | 1852, 1903 |
| DraI | TTTAAA | 1 cut(s) | 1723 |
| EaeI | YGGCCR | 1 cut(s) | 1020 |
| Eam1104I | CTCTTC | 1 cut(s) | 1782 |
| EarI | CTCTTC | 1 cut(s) | 1782 |
| Ecl136II | GAGCTC | 1 cut(s) | 2119 |
| Eco130I | CCWWGG | 3 cut(s) | 1094, 1743, 2007 |
| Eco24I | GRGCYC | 3 cut(s) | 1808, 1962, 2121 |
| Eco31I | GGTCTC | 1 cut(s) | 1406 |
| Eco32I | GATATC | 1 cut(s) | 1569 |
| Eco47I | GGWCC | 3 cut(s) | 1601, 1674, 2162 |
| Eco53kI | GAGCTC | 1 cut(s) | 2119 |
| Eco57I | CTGAAG | 4 cut(s) | 77, 717, 792, 1649 |
| Eco88I | CYCGRG | 4 cut(s) | 530, 723, 1596, 1955 |
| EcoICRI | GAGCTC | 1 cut(s) | 2119 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 96 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1601 |
| EcoRII | CCWGG | 3 cut(s) | 97, 1152, 1648 |
| EcoRV | GATATC | 1 cut(s) | 1569 |
| EcoT14I | CCWWGG | 3 cut(s) | 1094, 1743, 2007 |
| EcoT38I | GRGCYC | 3 cut(s) | 1808, 1962, 2121 |
| ErhI | CCWWGG | 3 cut(s) | 1094, 1743, 2007 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2299, 2331 |
| FaqI | GGGAC | 3 cut(s) | 813, 1660, 2271 |
| FauNDI | CATATG | 3 cut(s) | 81, 253, 590 |
| FblI | GTMKAC | 3 cut(s) | 444, 1190, 1453 |
| FokI | GGATG | 9 cut(s) | 742, 1001, 1210, 1214, 1342, 1442, 1775, 1976, 2143 |
| FriOI | GRGCYC | 3 cut(s) | 1808, 1962, 2121 |
| FspBI | CTAG | 3 cut(s) | 420, 953, 2049 |
| GlaI | GCGC | 2 cut(s) | 1885, 2035 |
| GsaI | CCCAGC | 2 cut(s) | 1392, 1425 |
| GsuI | CTGGAG | 3 cut(s) | 81, 1695, 2285 |
| HaeII | RGCGCY | 1 cut(s) | 1887 |
| HaeIII | GGCC | 4 cut(s) | 51, 529, 1022, 1062 |
| HapII | CCGG | 6 cut(s) | 372, 768, 1272, 1398, 1956, 2205 |
| HhaI | GCGC | 2 cut(s) | 1886, 2036 |
| Hin6I | GCGC | 2 cut(s) | 1884, 2034 |
| HinP1I | GCGC | 2 cut(s) | 1884, 2034 |
| HindIII | AAGCTT | 2 cut(s) | 541, 790 |
| HinfI | GANTC | 9 cut(s) | 230, 244, 284, 446, 478, 507, 533, 1029, 1730 |
| HpaII | CCGG | 6 cut(s) | 372, 768, 1272, 1398, 1956, 2205 |
| HphI | GGTGA | 3 cut(s) | 103, 227, 2098 |
| Hpy188I | TCNGA | 4 cut(s) | 229, 1447, 1893, 1984 |
| Hpy188III | TCNNGA | 7 cut(s) | 404, 596, 735, 810, 1734, 2114, 2142 |
| HpyAV | CCTTC | 5 cut(s) | 52, 161, 593, 1629, 2109 |
| HpyCH4IV | ACGT | 1 cut(s) | 936 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 24, 1394, 1883, 2033, 2058 |
| HpyF3I | CTNAG | 8 cut(s) | 216, 228, 886, 1033, 1413, 1878, 1890, 1981 |
| HpySE526I | ACGT | 1 cut(s) | 936 |
| HspAI | GCGC | 2 cut(s) | 1884, 2034 |
| KroI | GCCGGC | 1 cut(s) | 2204 |
| KroNI | GCCGGC | 1 cut(s) | 2206 |
| Kzo9I | GATC | 2 cut(s) | 1852, 1903 |
| LguI | GCTCTTC | 1 cut(s) | 1782 |
| Lsp1109I | GCAGC | 9 cut(s) | 313, 751, 866, 889, 894, 1124, 1351, 1630, 1823 |
| LweI | GCATC | 7 cut(s) | 718, 904, 956, 1243, 1253, 1797, 2061 |
| MaeI | CTAG | 3 cut(s) | 420, 953, 2049 |
| MaeII | ACGT | 1 cut(s) | 936 |
| MaeIII | GTNAC | 2 cut(s) | 109, 2149 |
| MalI | GATC | 2 cut(s) | 1854, 1905 |
| MboI | GATC | 2 cut(s) | 1852, 1903 |
| MhlI | GDGCHC | 5 cut(s) | 649, 832, 1808, 1962, 2121 |
| MlsI | TGGCCA | 1 cut(s) | 1022 |
| MluNI | TGGCCA | 1 cut(s) | 1022 |
| MlyI | GAGTC | 5 cut(s) | 239, 440, 487, 516, 542 |
| MmeI | TCCRAC | 2 cut(s) | 407, 560 |
| Mox20I | TGGCCA | 1 cut(s) | 1022 |
| MroNI | GCCGGC | 1 cut(s) | 2204 |
| MroXI | GAANNNNTTC | 3 cut(s) | 288, 1211, 2311 |
| MscI | TGGCCA | 1 cut(s) | 1022 |
| MseI | TTAA | 8 cut(s) | 660, 1535, 1722, 1782, 2085, 2169, 2217, 2223 |
| MslI | CAYNNNNRTG | 1 cut(s) | 80 |
| Msp20I | TGGCCA | 1 cut(s) | 1022 |
| MspI | CCGG | 6 cut(s) | 372, 768, 1272, 1398, 1956, 2205 |
| MspR9I | CCNGG | 7 cut(s) | 99, 372, 1154, 1273, 1650, 1956, 1957 |
| Mva1269I | GAATGC | 1 cut(s) | 1583 |
| MvaI | CCWGG | 3 cut(s) | 99, 1154, 1650 |
| MvnI | CGCG | 1 cut(s) | 2190 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 24, 1394, 1883, 2033, 2058 |
| NaeI | GCCGGC | 1 cut(s) | 2206 |
| NciI | CCSGG | 4 cut(s) | 372, 1273, 1956, 1957 |
| NcoI | CCATGG | 2 cut(s) | 1743, 2007 |
| NdeI | CATATG | 3 cut(s) | 81, 253, 590 |
| NdeII | GATC | 2 cut(s) | 1852, 1903 |
| NgoMIV | GCCGGC | 1 cut(s) | 2204 |
| NheI | GCTAGC | 1 cut(s) | 2048 |
| NlaIV | GGNNCC | 6 cut(s) | 87, 149, 1396, 1603, 1676, 1805 |
| NmuCI | GTSAC | 2 cut(s) | 109, 2149 |
| NspI | RCATGY | 3 cut(s) | 31, 1931, 2061 |
| PaeR7I | CTCGAG | 1 cut(s) | 1596 |
| PagI | TCATGA | 1 cut(s) | 2113 |
| PaqCI | CACCTGC | 1 cut(s) | 2209 |
| PasI | CCCWGGG | 1 cut(s) | 1153 |
| PciI | ACATGT | 1 cut(s) | 1927 |
| PciSI | GCTCTTC | 1 cut(s) | 1782 |
| PctI | GAATGC | 1 cut(s) | 1583 |
| PdiI | GCCGGC | 1 cut(s) | 2206 |
| PdmI | GAANNNNTTC | 3 cut(s) | 288, 1211, 2311 |
| PfeI | GAWTC | 4 cut(s) | 244, 284, 1029, 1730 |
| PleI | GAGTC | 5 cut(s) | 238, 440, 486, 515, 541 |
| PpsI | GAGTC | 5 cut(s) | 238, 440, 486, 515, 541 |
| PpuMI | RGGWCCY | 1 cut(s) | 1601 |
| PscI | ACATGT | 1 cut(s) | 1927 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2075 |
| Psp124BI | GAGCTC | 1 cut(s) | 2121 |
| Psp5II | RGGWCCY | 1 cut(s) | 1601 |
| Psp6I | CCWGG | 3 cut(s) | 97, 1152, 1648 |
| PspFI | CCCAGC | 2 cut(s) | 1388, 1421 |
| PspGI | CCWGG | 3 cut(s) | 97, 1152, 1648 |
| PspN4I | GGNNCC | 6 cut(s) | 87, 149, 1396, 1603, 1676, 1805 |
| PspPI | GGNCC | 5 cut(s) | 528, 1061, 1601, 1674, 2162 |
| PspPPI | RGGWCCY | 1 cut(s) | 1601 |
| PspXI | VCTCGAGB | 1 cut(s) | 1596 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1199, 1231 |
| PstI | CTGCAG | 4 cut(s) | 138, 331, 884, 1813 |
| RsaI | GTAC | 6 cut(s) | 296, 369, 745, 1303, 1626, 2330 |
| RsaNI | GTAC | 6 cut(s) | 295, 368, 744, 1302, 1625, 2329 |
| RseI | CAYNNNNRTG | 1 cut(s) | 80 |
| SacI | GAGCTC | 1 cut(s) | 2121 |
| SapI | GCTCTTC | 1 cut(s) | 1782 |
| SaqAI | TTAA | 8 cut(s) | 660, 1535, 1722, 1782, 2085, 2169, 2217, 2223 |
| Sau3AI | GATC | 2 cut(s) | 1852, 1903 |
| Sau96I | GGNCC | 5 cut(s) | 528, 1061, 1601, 1674, 2162 |
| SchI | GAGTC | 5 cut(s) | 239, 440, 487, 516, 542 |
| ScrFI | CCNGG | 7 cut(s) | 99, 372, 1154, 1273, 1650, 1956, 1957 |
| SduI | GDGCHC | 5 cut(s) | 649, 832, 1808, 1962, 2121 |
| SfaNI | GCATC | 7 cut(s) | 718, 904, 956, 1243, 1253, 1797, 2061 |
| SfcI | CTRYAG | 5 cut(s) | 134, 327, 880, 1170, 1809 |
| Sfr274I | CTCGAG | 1 cut(s) | 1596 |
| SinI | GGWCC | 3 cut(s) | 1601, 1674, 2162 |
| SlaI | CTCGAG | 1 cut(s) | 1596 |
| SmaI | CCCGGG | 1 cut(s) | 1957 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 80 |
| SmlI | CTYRAG | 3 cut(s) | 345, 1596, 2120 |
| SmoI | CTYRAG | 3 cut(s) | 345, 1596, 2120 |
| SsiI | CCGC | 1 cut(s) | 276 |
| SspMI | CTAG | 3 cut(s) | 420, 953, 2049 |
| SstI | GAGCTC | 1 cut(s) | 2121 |
| StyD4I | CCNGG | 7 cut(s) | 97, 370, 1152, 1271, 1648, 1954, 1955 |
| StyI | CCWWGG | 3 cut(s) | 1094, 1743, 2007 |
| TaiI | ACGT | 1 cut(s) | 939 |
| TaqI | TCGA | 5 cut(s) | 1125, 1597, 1872, 1911, 2069 |
| TatI | WGTACW | 2 cut(s) | 294, 2328 |
| TauI | GCSGC | 1 cut(s) | 278 |
| TfiI | GAWTC | 4 cut(s) | 244, 284, 1029, 1730 |
| Tru1I | TTAA | 8 cut(s) | 660, 1535, 1722, 1782, 2085, 2169, 2217, 2223 |
| Tru9I | TTAA | 8 cut(s) | 660, 1535, 1722, 1782, 2085, 2169, 2217, 2223 |
| TscAI | CASTG | 6 cut(s) | 523, 766, 1111, 1273, 1366, 2376 |
| TseFI | GTSAC | 2 cut(s) | 109, 2149 |
| TseI | GCWGC | 9 cut(s) | 326, 764, 879, 882, 902, 1112, 1364, 1643, 1811 |
| Tsp45I | GTSAC | 2 cut(s) | 109, 2149 |
| TspDTI | ATGAA | 5 cut(s) | 17, 684, 1001, 2102, 2336 |
| TspGWI | ACGGA | 2 cut(s) | 1329, 1982 |
| TspMI | CCCGGG | 1 cut(s) | 1955 |
| TspRI | CASTG | 6 cut(s) | 523, 766, 1111, 1273, 1366, 2376 |
| VneI | GTGCAC | 1 cut(s) | 645 |
| VpaK11BI | GGWCC | 3 cut(s) | 1601, 1674, 2162 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 96 |
| XapI | RAATTY | 5 cut(s) | 522, 677, 710, 1084, 1319 |
| XceI | RCATGY | 3 cut(s) | 31, 1931, 2061 |
| XhoI | CTCGAG | 1 cut(s) | 1596 |
| XmaI | CCCGGG | 1 cut(s) | 1955 |
| XmiI | GTMKAC | 3 cut(s) | 444, 1190, 1453 |
| XmnI | GAANNNNTTC | 3 cut(s) | 288, 1211, 2311 |
| XspI | CTAG | 3 cut(s) | 420, 953, 2049 |