MD05G1087100.v1.1

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013455
Physical Location & Seq
Forward (+)
18551201 .. 18558118
6918 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1087100.v1.1.491

Sequence Viewer

Length: 1839 bp
ATGGATGCAGAACCTGCTGACTCCTCACCTGCACCTCCAGTTAATGCTCCCGAATCACAACCTCCTCCTGCCACAGACTCTGGATCAGATTCTCATGGTGCACCAACTAAGCAATCTTCTGGCTTGTCATCATGGGCCAAAAATCTGAAACTTCATCAGCCATTGGCACCAACGCAGGACAACTCTCAGTCTGGGAATAGTGGACAATCGAGTTTTTCACGCTTTACTAGTGGGTTAGGATTGCGTTTGTCTCCAAAAGCTCCACCATCAGATGATATTTCAAATGAAAGTCCAACAACTCTGCAACCTGGTTTTATTGGAACAATTACGAAAGGGATAGTTGACTCTTCTAAGAGTGCAGTGAAAGCTGTTCAGGTTAAAGCCAGGCATGTTGTCTCTCAAAACAAACGAAGATACCAGGAAGGAGGATTTGATTTGGACATGACTTATATTACGGAGAATATAATTGCCATGGGATTCCCTGCTGGTGATATGAGCTCTGGTTTTTTCGGATATGTTGAGGGGTTCTACAGAAATCATATGGAAGAAGTGATTAAGTTCTTTGAAACTAATCATAAGGACAAGTACAAAGTATATAACCTTTGTTCTGAAAGGCTTTATGATGCGTCATTATTTGAAGGAAAGGTGGCTAGTTTCCCATTTGATGACCATAATTGTCCGCCAATTCATCTAATAACATTATTTTGTCGAAGTGCTTATGCATGGCTGAAAGAGGATATTGAGAATGTTGTGGTTGTGCATTGCAAGGCCGGAATGGCAAGAACAGGACTGATGATTTCCAGTCTTCTCCTATACTTAAAGTTCTTTCCGACCGCTGAGGAGTCTATTGACTACTACAACCAGAAACGATGCTTTGATGGAAAAGGGCTTGTTCTGCCGAGTCAGATTAGGTACGTTAAATATTTTGAACGTATCTTAACATACTTCAACGGTGAAAACCCACCTGGGCGCAGGTGCATGCTTAGGGGATTTCGGCTTCATAGGTGCCCATACTGGATCAGGCCCTCCATTACTGTATCTGATCATAACGGTGTTCTCTTCTCCACAAAAGAACATCCTAGAACAAAAGGTCTTTCGCCAGAAGATTATTGGTTTTCTGCTCCAAAGAAGGGTGTAATGGTCTTTGCCCTGCCGGGGGAACTTGGTTTGACAGAGTTAGTTGGGGACTTCAAAGTCCATTTTCATGATCGTCAAGGAGATTTTTACTGCTGGCTGAACACAACAATGACAGAAAATAGAAAAGTTTTAACCAACAATGATCTCGATGGGTTTGACAAGAGAAAACTTCCTTCCCCAGGGTTTCAGGTTGAGGTGGTTTTAGTGGATTATAGCGGCAATGTTCCTACGACACCTAATACTGAAACTTCTGCTAAGAAACCAGATGAAAGCTCAGGTACCAAACCTTCCTCGGATGAGGGAGGTGCAGCTGCTGCTCCACAAGAAAGTAAAAGCCCCGGAAGTCAAGACAAGGATGATGTGTTCTCAGATAGTGAGGCAGAGGAAGGTGGCTCTGCTAAGAGCCGGCAAGCAGAAGCAACTTCTCCACCCACAGGTGTACCTGTGGCCAGCACCAGCACCAGTTCCGAGTCTGATACCAAACCAGATCAAATTTCAAGTTTAACAAATGTGACCAAGCAAGTTTCTATTGGAAACACGGACTCCAAACAGGGAGCTGCTGCTAGTGAGCCTAAAAGTTCTGTTAGTGGTGGAAATGCCCCAGGCCTTGAGGCTTCGAGCACAGAAAGTGAATTTAAGGCAATGGCTGCTGATGCCTCCGTTTTCTCGTTTGGAGATGACGAGGACTCTGAAAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001931 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004725 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005938 GO:0006464 GO:0006469 GO:0006470 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007154 GO:0007163 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008104 GO:0008150 GO:0008152 GO:0008154 GO:0008289 GO:0009267 GO:0009605 GO:0009628 GO:0009651 GO:0009892 GO:0009987 GO:0009991 GO:0010563 GO:0010605 GO:0014065 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019637 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0023052 GO:0030010 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030041 GO:0030258 GO:0030587 GO:0031152 GO:0031254 GO:0031257 GO:0031268 GO:0031269 GO:0031272 GO:0031279 GO:0031323 GO:0031324 GO:0031344 GO:0031399 GO:0031400 GO:0031667 GO:0031668 GO:0031669 GO:0032153 GO:0032155 GO:0032268 GO:0032269 GO:0032502 GO:0032796 GO:0032879 GO:0033036 GO:0033554 GO:0033673 GO:0034461 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0034613 GO:0034622 GO:0035335 GO:0035556 GO:0036051 GO:0036052 GO:0036211 GO:0040011 GO:0042221 GO:0042325 GO:0042326 GO:0042330 GO:0042578 GO:0042594 GO:0042995 GO:0043086 GO:0043167 GO:0043168 GO:0043170 GO:0043327 GO:0043412 GO:0043549 GO:0043933 GO:0044085 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0045761 GO:0045859 GO:0045936 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048015 GO:0048017 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0050919 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051248 GO:0051258 GO:0051270 GO:0051272 GO:0051285 GO:0051286 GO:0051301 GO:0051338 GO:0051339 GO:0051348 GO:0051641 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0051800 GO:0052866 GO:0060255 GO:0060491 GO:0061640 GO:0065003 GO:0065007 GO:0065009 GO:0070300 GO:0070727 GO:0071496 GO:0071704 GO:0071840 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0090702 GO:0097435 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0106017 GO:0120025 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140096 GO:1901564 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
ko00562 ko01521 ko03050 ko04068 ko04070 ko04071 ko04115 ko04140 ko04150 ko04151 ko04212 ko04218 ko04510 ko04931 ko05161 ko05165 ko05169 ko05200 ko05206 ko05213 ko05214 ko05215 ko05218 ko05222 ko05224 ko05225 ko05230 map00562 map01521 map03050 map04068 map04070 map04071 map04115 map04140 map04150 map04151 map04212 map04218 map04510 map04931 map05161 map05165 map05169 map05200 map05206 map05213 map05214 map05215 map05218 map05222 map05224 map05225 map05230
Pfam Domains
Protein Families
DSPc

Protein Analysis

613

Amino Acids

66.58

Weight (kDa)

5.35

Isoelectric Point (pI)

55.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tc-R-P PF22785 217 - 271 1.8e-06 Polymorphic toxin system, DSP-PTPase phosphatase
PTEN2_C2 PF22918 323 - 451 8.1e-73 PTEN2, C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19420
fragaria_vesca FvH4_2g05490 FvH4_2g05490
malus_domestica MD00G1045500.v1.1 MD05G1087100.v1.1
prunus_persica Prupe.8G131700_v2.0.a1
pyrus_communis pycom05g08640 pycom10g08170
rosa_chinensis RchiOBHm_Chr6g0255761
rosa_laevigata RLG00000014815
rosa_roxburghii Rroxscaffold_7G00209690
rosa_rugosa Rorug05G0561200 Rorug05G0561300
rosa_samantha Rh6AG079200 Rh6BG072300 Rh6CG070500 Rh6DG067700
rosa_wichuraiana Rw6G006830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 37, 963
AasI GACNNNNNNGTC 1 cut(s) 1193
Acc36I ACCTGC 3 cut(s) 22, 37, 963
Acc65I GGTACC 1 cut(s) 1415
AccB1I GGYRCC 3 cut(s) 166, 1005, 1415
AciI CCGC 3 cut(s) 680, 834, 1353
AclWI GGATC 2 cut(s) 91, 1025
AcoI YGGCCR 1 cut(s) 1584
AcsI RAATTY 2 cut(s) 1629, 1769
AfaI GTAC 4 cut(s) 587, 914, 1417, 1578
AfiI CCNNNNNNNGG 5 cut(s) 1130, 1155, 1156, 1316, 1571
AgsI TTSAA 7 cut(s) 282, 566, 638, 929, 949, 1192, 1635
AhlI ACTAGT 1 cut(s) 227
AjnI CCWGG 6 cut(s) 307, 383, 417, 964, 1315, 1738
AloI GAACNNNNNNTCC 2 cut(s) 1484, 1516
AluBI AGCT 6 cut(s) 260, 368, 498, 1410, 1448, 1694
AluI AGCT 6 cut(s) 260, 368, 498, 1410, 1448, 1694
Alw21I GWGCWC 3 cut(s) 103, 500, 1760
Alw26I GTCTC 2 cut(s) 255, 400
Alw44I GTGCAC 1 cut(s) 99
AlwI GGATC 2 cut(s) 91, 1025
AlwNI CAGNNNCTG 3 cut(s) 14, 80, 1451
AoxI GGCC 5 cut(s) 135, 768, 1022, 1584, 1741
ApaLI GTGCAC 1 cut(s) 99
ApeKI GCWGC 6 cut(s) 1445, 1448, 1451, 1694, 1697, 1784
ApoI RAATTY 2 cut(s) 1629, 1769
Asp718I GGTACC 1 cut(s) 1415
AspLEI GCGC 1 cut(s) 972
AspS9I GGNCC 2 cut(s) 135, 1023
AsuC2I CCSGG 2 cut(s) 1155, 1476
AsuHPI GGTGA 3 cut(s) 18, 500, 965
BaeGI GKGCMC 2 cut(s) 103, 1010
BaeI ACNNNNGTAYC 2 cut(s) 1560, 1593
BalI TGGCCA 1 cut(s) 1586
BanI GGYRCC 3 cut(s) 166, 1005, 1415
BanII GRGCYC 1 cut(s) 500
BarI GAAGNNNNNNTAC 2 cut(s) 1408, 1440
BbsI GAAGAC 1 cut(s) 797
Bbv12I GWGCWC 3 cut(s) 103, 500, 1760
BbvCI CCTCAGC 1 cut(s) 837
BbvI GCAGC 6 cut(s) 1435, 1438, 1457, 1681, 1684, 1771
BccI CCATC 3 cut(s) 274, 872, 1280
BciT130I CCWGG 6 cut(s) 309, 385, 419, 966, 1317, 1740
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 2 cut(s) 1155, 1476
BcoDI GTCTC 2 cut(s) 255, 400
BcuI ACTAGT 1 cut(s) 227
BfaI CTAG 4 cut(s) 228, 651, 1080, 1701
BfmI CTRYAG 1 cut(s) 529
BfuAI ACCTGC 3 cut(s) 22, 37, 963
BglI GCCNNNNNGGC 1 cut(s) 776
BisI GCNGC 7 cut(s) 1354, 1446, 1449, 1452, 1695, 1698, 1785
BlsI GCNGC 7 cut(s) 1355, 1447, 1450, 1453, 1696, 1699, 1786
Bme1390I CCNGG 8 cut(s) 309, 385, 419, 966, 1155, 1317, 1476, 1740
BmgT120I GGNCC 2 cut(s) 135, 1023
BmiI GGNNCC 3 cut(s) 168, 1007, 1417
BmrFI CCNGG 8 cut(s) 309, 385, 419, 966, 1155, 1317, 1476, 1740
BmsI GCATC 3 cut(s) 613, 860, 1780
BpiI GAAGAC 1 cut(s) 797
BpmI CTGGAG 1 cut(s) 21
Bpu10I CCTNAGC 3 cut(s) 837, 983, 1411
BpuEI CTTGAG 1 cut(s) 1766
BpuMI CCSGG 2 cut(s) 1155, 1476
BsaJI CCNNGG 8 cut(s) 471, 965, 1154, 1315, 1316, 1428, 1474, 1738
BsaXI ACNNNNNCTCC 2 cut(s) 1664, 1694
Bsc4I CCNNNNNNNGG 5 cut(s) 1130, 1155, 1156, 1316, 1571
Bse118I RCCGGY 1 cut(s) 1542
Bse1I ACTGG 4 cut(s) 38, 801, 1019, 1599
Bse3DI GCAATG 3 cut(s) 760, 1363, 1785
BseBI CCWGG 6 cut(s) 309, 385, 419, 966, 1317, 1740
BseDI CCNNGG 8 cut(s) 471, 965, 1154, 1315, 1316, 1428, 1474, 1738
BseGI GGATG 4 cut(s) 10, 1075, 1438, 1498
BseLI CCNNNNNNNGG 5 cut(s) 1130, 1155, 1156, 1316, 1571
BseMI GCAATG 3 cut(s) 760, 1363, 1785
BseMII CTCAG 4 cut(s) 200, 828, 1425, 1518
BseNI ACTGG 4 cut(s) 38, 801, 1019, 1599
BseRI GAGGAG 3 cut(s) 13, 54, 854
BseSI GKGCMC 2 cut(s) 103, 1010
BseXI GCAGC 6 cut(s) 1435, 1438, 1457, 1681, 1684, 1771
BsgI GTGCAG 3 cut(s) 15, 378, 1464
Bsh1285I CGRYCG 1 cut(s) 834
BshFI GGCC 5 cut(s) 137, 770, 1024, 1586, 1743
BshNI GGYRCC 3 cut(s) 166, 1005, 1415
BsiEI CGRYCG 1 cut(s) 834
BsiHKAI GWGCWC 3 cut(s) 103, 500, 1760
BsiSI CCGG 4 cut(s) 771, 1154, 1476, 1543
BslFI GGGAC 1 cut(s) 1199
BslI CCNNNNNNNGG 5 cut(s) 1130, 1155, 1156, 1316, 1571
BsmAI GTCTC 2 cut(s) 255, 400
BsmFI GGGAC 1 cut(s) 1199
BsnI GGCC 5 cut(s) 137, 770, 1024, 1586, 1743
Bsp1286I GDGCHC 4 cut(s) 103, 500, 1010, 1760
Bsp143I GATC 6 cut(s) 83, 1017, 1042, 1207, 1279, 1624
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 3 cut(s) 680, 834, 1353
BspANI GGCC 5 cut(s) 137, 770, 1024, 1586, 1743
BspCNI CTCAG 4 cut(s) 199, 829, 1424, 1517
BspHI TCATGA 1 cut(s) 1204
BspLI GGNNCC 3 cut(s) 168, 1007, 1417
BspMI ACCTGC 3 cut(s) 22, 37, 963
BspPI GGATC 2 cut(s) 91, 1025
BspT107I GGYRCC 3 cut(s) 166, 1005, 1415
BsrDI GCAATG 3 cut(s) 760, 1363, 1785
BsrFI RCCGGY 1 cut(s) 1542
BsrI ACTGG 4 cut(s) 38, 801, 1019, 1599
BssAI RCCGGY 1 cut(s) 1542
BssECI CCNNGG 8 cut(s) 471, 965, 1154, 1315, 1316, 1428, 1474, 1738
BssMI GATC 6 cut(s) 83, 1017, 1042, 1207, 1279, 1624
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 6 cut(s) 309, 385, 419, 966, 1317, 1740
Bst4CI ACNGT 3 cut(s) 953, 1036, 1052
Bst6I CTCTTC 2 cut(s) 352, 1064
BstAPI GCANNNNNTGC 3 cut(s) 14, 1451, 1784
BstC8I GCNNGC 5 cut(s) 980, 1232, 1544, 1548, 1588
BstDEI CTNAG 9 cut(s) 108, 186, 351, 837, 983, 1392, 1411, 1504, 1536
BstDSI CCRYGG 1 cut(s) 471
BstENI CCTNNNNNAGG 1 cut(s) 1314
BstF5I GGATG 4 cut(s) 10, 1075, 1438, 1498
BstHHI GCGC 1 cut(s) 972
BstKTI GATC 6 cut(s) 86, 1020, 1045, 1210, 1282, 1627
BstMAI GTCTC 2 cut(s) 255, 400
BstMBI GATC 6 cut(s) 83, 1017, 1042, 1207, 1279, 1624
BstMCI CGRYCG 1 cut(s) 834
BstMWI GCNNNNNNNGC 7 cut(s) 14, 365, 776, 895, 1451, 1784, 1790
BstNI CCWGG 6 cut(s) 309, 385, 419, 966, 1317, 1740
BstNSI RCATGY 2 cut(s) 392, 982
BstSCI CCNGG 8 cut(s) 307, 383, 417, 964, 1153, 1315, 1474, 1738
BstSFI CTRYAG 1 cut(s) 529
BstSLI GKGCMC 2 cut(s) 103, 1010
BstV1I GCAGC 6 cut(s) 1435, 1438, 1457, 1681, 1684, 1771
BstV2I GAAGAC 1 cut(s) 797
BsuRI GGCC 5 cut(s) 137, 770, 1024, 1586, 1743
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 4 cut(s) 10, 1075, 1438, 1498
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 366
BveI ACCTGC 3 cut(s) 22, 37, 963
Cac8I GCNNGC 5 cut(s) 980, 1232, 1544, 1548, 1588
CaiI CAGNNNCTG 3 cut(s) 14, 80, 1451
CciI TCATGA 1 cut(s) 1204
CfoI GCGC 1 cut(s) 972
Cfr10I RCCGGY 1 cut(s) 1542
Cfr13I GGNCC 2 cut(s) 135, 1023
CseI GACGC 1 cut(s) 615
CsiI ACCWGGT 1 cut(s) 307
Csp6I GTAC 4 cut(s) 586, 913, 1416, 1577
CviAII CATG 8 cut(s) 95, 132, 389, 442, 472, 723, 979, 1205
CviQI GTAC 4 cut(s) 586, 913, 1416, 1577
DdeI CTNAG 9 cut(s) 108, 186, 351, 837, 983, 1392, 1411, 1504, 1536
DpnI GATC 6 cut(s) 85, 1019, 1044, 1209, 1281, 1626
DpnII GATC 6 cut(s) 83, 1017, 1042, 1207, 1279, 1624
DrdI GACNNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 1 cut(s) 1584
Eam1104I CTCTTC 2 cut(s) 352, 1064
EarI CTCTTC 2 cut(s) 352, 1064
EciI GGCGGA 1 cut(s) 669
Ecl136II GAGCTC 1 cut(s) 498
Eco130I CCWWGG 1 cut(s) 471
Eco147I AGGCCT 1 cut(s) 1743
Eco24I GRGCYC 1 cut(s) 500
Eco53kI GAGCTC 1 cut(s) 498
EcoICRI GAGCTC 1 cut(s) 498
EcoNI CCTNNNNNAGG 1 cut(s) 1314
EcoO109I RGGNCCY 1 cut(s) 1023
EcoRII CCWGG 6 cut(s) 307, 383, 417, 964, 1315, 1738
EcoT14I CCWWGG 1 cut(s) 471
EcoT22I ATGCAT 1 cut(s) 724
EcoT38I GRGCYC 1 cut(s) 500
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 8 cut(s) 98, 135, 392, 445, 475, 726, 982, 1208
FaqI GGGAC 1 cut(s) 1199
FatI CATG 8 cut(s) 94, 131, 388, 441, 471, 722, 978, 1204
FauNDI CATATG 1 cut(s) 540
FbaI TGATCA 1 cut(s) 1042
Fnu4HI GCNGC 7 cut(s) 1354, 1446, 1449, 1452, 1695, 1698, 1785
FokI GGATG 4 cut(s) 17, 1062, 1445, 1505
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 7 cut(s) 1354, 1446, 1449, 1452, 1695, 1698, 1785
FspBI CTAG 4 cut(s) 228, 651, 1080, 1701
GlaI GCGC 1 cut(s) 971
GluI GCNGC 7 cut(s) 1354, 1446, 1449, 1452, 1695, 1698, 1785
GsuI CTGGAG 1 cut(s) 21
HaeIII GGCC 5 cut(s) 137, 770, 1024, 1586, 1743
HapII CCGG 4 cut(s) 771, 1154, 1476, 1543
HgaI GACGC 1 cut(s) 615
HhaI GCGC 1 cut(s) 972
Hin1II CATG 8 cut(s) 98, 135, 392, 445, 475, 726, 982, 1208
Hin6I GCGC 1 cut(s) 970
HinP1I GCGC 1 cut(s) 970
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HpaII CCGG 4 cut(s) 771, 1154, 1476, 1543
HphI GGTGA 3 cut(s) 18, 500, 965
Hpy166II GTNNAC 4 cut(s) 101, 203, 343, 1577
Hpy188III TCNNGA 5 cut(s) 50, 81, 1205, 1283, 1484
Hpy8I GTNNAC 4 cut(s) 101, 203, 343, 1577
HpyAV CCTTC 6 cut(s) 416, 632, 1123, 1320, 1434, 1517
HpyCH4III ACNGT 3 cut(s) 953, 1036, 1052
HpyCH4IV ACGT 2 cut(s) 915, 931
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 365, 776, 895, 1451, 1784, 1790
HpyF3I CTNAG 9 cut(s) 108, 186, 351, 837, 983, 1392, 1411, 1504, 1536
HpySE526I ACGT 2 cut(s) 915, 931
Hsp92II CATG 8 cut(s) 98, 135, 392, 445, 475, 726, 982, 1208
HspAI GCGC 1 cut(s) 970
KpnI GGTACC 1 cut(s) 1419
KroI GCCGGC 1 cut(s) 1542
KroNI GCCGGC 1 cut(s) 1544
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 6 cut(s) 83, 1017, 1042, 1207, 1279, 1624
LmnI GCTCC 5 cut(s) 52, 265, 1126, 1459, 1691
Lsp1109I GCAGC 6 cut(s) 1435, 1438, 1457, 1681, 1684, 1771
LweI GCATC 3 cut(s) 613, 860, 1780
MabI ACCWGGT 1 cut(s) 307
MaeI CTAG 4 cut(s) 228, 651, 1080, 1701
MaeII ACGT 2 cut(s) 915, 931
MaeIII GTNAC 1 cut(s) 1648
MalI GATC 6 cut(s) 85, 1019, 1044, 1209, 1281, 1626
MboI GATC 6 cut(s) 83, 1017, 1042, 1207, 1279, 1624
MboII GAAGA 7 cut(s) 108, 339, 423, 557, 797, 1051, 1115
MhlI GDGCHC 4 cut(s) 103, 500, 1010, 1760
MlsI TGGCCA 1 cut(s) 1586
MluCI AATT 6 cut(s) 324, 465, 673, 684, 1629, 1769
MluNI TGGCCA 1 cut(s) 1586
MlyI GAGTC 8 cut(s) 14, 71, 338, 851, 910, 1616, 1673, 1817
MmeI TCCRAC 2 cut(s) 317, 854
Mox20I TGGCCA 1 cut(s) 1586
Mph1103I ATGCAT 1 cut(s) 724
MroNI GCCGGC 1 cut(s) 1542
MscI TGGCCA 1 cut(s) 1586
MseI TTAA 9 cut(s) 42, 378, 555, 818, 918, 938, 1268, 1640, 1773
MslI CAYNNNNRTG 3 cut(s) 1050, 1203, 1244
Msp20I TGGCCA 1 cut(s) 1586
MspA1I CMGCKG 2 cut(s) 836, 1448
MspI CCGG 4 cut(s) 771, 1154, 1476, 1543
MspR9I CCNGG 8 cut(s) 309, 385, 419, 966, 1155, 1317, 1476, 1740
MvaI CCWGG 6 cut(s) 309, 385, 419, 966, 1317, 1740
MwoI GCNNNNNNNGC 7 cut(s) 14, 365, 776, 895, 1451, 1784, 1790
NaeI GCCGGC 1 cut(s) 1544
NciI CCSGG 2 cut(s) 1155, 1476
NcoI CCATGG 1 cut(s) 471
NdeI CATATG 1 cut(s) 540
NdeII GATC 6 cut(s) 83, 1017, 1042, 1207, 1279, 1624
NgoMIV GCCGGC 1 cut(s) 1542
NlaIII CATG 8 cut(s) 98, 135, 392, 445, 475, 726, 982, 1208
NlaIV GGNNCC 3 cut(s) 168, 1007, 1417
NmeAIII GCCGAG 1 cut(s) 924
NmuCI GTSAC 1 cut(s) 1648
NsiI ATGCAT 1 cut(s) 724
NspI RCATGY 2 cut(s) 392, 982
PaeI GCATGC 1 cut(s) 982
PagI TCATGA 1 cut(s) 1204
PaqCI CACCTGC 2 cut(s) 37, 963
PasI CCCWGGG 1 cut(s) 1316
PceI AGGCCT 1 cut(s) 1743
PdiI GCCGGC 1 cut(s) 1544
PfeI GAWTC 3 cut(s) 53, 89, 477
PkrI GCNGC 7 cut(s) 1355, 1447, 1450, 1453, 1696, 1699, 1786
PleI GAGTC 8 cut(s) 14, 71, 338, 850, 909, 1615, 1673, 1817
PpsI GAGTC 8 cut(s) 14, 71, 338, 850, 909, 1615, 1673, 1817
Psp124BI GAGCTC 1 cut(s) 500
Psp6I CCWGG 6 cut(s) 307, 383, 417, 964, 1315, 1738
PspGI CCWGG 6 cut(s) 307, 383, 417, 964, 1315, 1738
PspN4I GGNNCC 3 cut(s) 168, 1007, 1417
PspPI GGNCC 2 cut(s) 135, 1023
PstNI CAGNNNCTG 3 cut(s) 14, 80, 1451
PvuII CAGCTG 1 cut(s) 1448
RsaI GTAC 4 cut(s) 587, 914, 1417, 1578
RsaNI GTAC 4 cut(s) 586, 913, 1416, 1577
RseI CAYNNNNRTG 3 cut(s) 1050, 1203, 1244
SacI GAGCTC 1 cut(s) 500
SaqAI TTAA 9 cut(s) 42, 378, 555, 818, 918, 938, 1268, 1640, 1773
SatI GCNGC 7 cut(s) 1354, 1446, 1449, 1452, 1695, 1698, 1785
Sau3AI GATC 6 cut(s) 83, 1017, 1042, 1207, 1279, 1624
Sau96I GGNCC 2 cut(s) 135, 1023
SchI GAGTC 8 cut(s) 14, 71, 338, 851, 910, 1616, 1673, 1817
ScrFI CCNGG 8 cut(s) 309, 385, 419, 966, 1155, 1317, 1476, 1740
SduI GDGCHC 4 cut(s) 103, 500, 1010, 1760
SexAI ACCWGGT 1 cut(s) 307
SfaNI GCATC 3 cut(s) 613, 860, 1780
SfcI CTRYAG 1 cut(s) 529
SmiMI CAYNNNNRTG 3 cut(s) 1050, 1203, 1244
SmlI CTYRAG 1 cut(s) 1745
SmoI CTYRAG 1 cut(s) 1745
SpeI ACTAGT 1 cut(s) 227
SphI GCATGC 1 cut(s) 982
Sse9I AATT 6 cut(s) 324, 465, 673, 684, 1629, 1769
SseBI AGGCCT 1 cut(s) 1743
SsiI CCGC 3 cut(s) 680, 834, 1353
SspI AATATT 1 cut(s) 923
SspMI CTAG 4 cut(s) 228, 651, 1080, 1701
SstI GAGCTC 1 cut(s) 500
StuI AGGCCT 1 cut(s) 1743
StyD4I CCNGG 8 cut(s) 307, 383, 417, 964, 1153, 1315, 1474, 1738
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 3 cut(s) 953, 1036, 1052
TaiI ACGT 2 cut(s) 918, 934
TaqI TCGA 4 cut(s) 209, 709, 1284, 1754
TasI AATT 6 cut(s) 324, 465, 673, 684, 1629, 1769
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 1356
TfiI GAWTC 3 cut(s) 53, 89, 477
Tru1I TTAA 9 cut(s) 42, 378, 555, 818, 918, 938, 1268, 1640, 1773
Tru9I TTAA 9 cut(s) 42, 378, 555, 818, 918, 938, 1268, 1640, 1773
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 1 cut(s) 1648
TseI GCWGC 6 cut(s) 1445, 1448, 1451, 1694, 1697, 1784
Tsp45I GTSAC 1 cut(s) 1648
TspDTI ATGAA 6 cut(s) 143, 300, 677, 989, 1193, 1419
TspGWI ACGGA 3 cut(s) 470, 1691, 1786
TspRI CASTG 1 cut(s) 366
VneI GTGCAC 1 cut(s) 99
XagI CCTNNNNNAGG 1 cut(s) 1314
XapI RAATTY 2 cut(s) 1629, 1769
XceI RCATGY 2 cut(s) 392, 982
XcmI CCANNNNNNNNNTGG 1 cut(s) 1107
XspI CTAG 4 cut(s) 228, 651, 1080, 1701
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.