Rorug05G0561300

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013455
Physical Location & Seq
Forward (+)
75144337 .. 75154260
9924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0561300.1

Sequence Viewer

Length: 2331 bp
ATGATGACAAGGATAGGGAACCGACAACGTGAAAGGGATCCCAGTGATAGATGTGGAAGGTGGAGGGGTGATCGTCAAGATAGGGATCGACACACTTCTGATGAAAATGGTGAGGTGGCAGAAGAAGACAGGGGTGGGCAAGAAAGAGGAAGGAAAAATGAAAATGGGAACAGACAACGTGAAAATGCGAATGGTGATAGGGGGAGAGAAAATAGAAGTTCTAAGGATTATGAGCGACTGAAGAGATACCGATATGGTAGTGGACATAGAAGGCGGGAGAATGAGGATGGTCGTAGAAACAGGGACAGGCATTCACGTCACTACTCTGAGCGCAGGGATGAAAGGGAAGAGCGTAAAACTTCAGAACGGAAAAGGGATAGTAGAGATGATGGTAGGCAAAGTAGGCATAGAAACCGGGATGACGATGGTGAGATAAAGAATCGCAGAAATAAGGAGGTGAATGAAAGACGAGAAGACACTGAGCGAGATGATAGGATGCTTGAGAATTCAAAACCTCAAGGACGGTCAACATTGCAGGAGGGAAATCTGAATAATAATGTGGCAAATTTGGGAAAGAGTGGTGGGGTTTATATTCCGCCCTATAAGTTGGCCCGAATGATGAAAGAGGTCGAAGATAAAAGTAGCGTCGAGTATCAACGCTTGACATGGGATGCCCTCCGAAAGAGTATCAATGGGCTTGTGAACAAGGTCAATGCTGCAAACATTAAAAATATAATTCCAGAAATTTTTGCAGAGAATCTCATTCGTGGAAGAGGTCTCTTTTGTCGATCCTGTATGAAGTCACAGATGGCATCTCCAGGATTTACAGACGTCTTTGCTGGCTTGGTTGCTGTTGTTAACACCAAGTTTCCTGAGGTTGGTGAACTTCTGTTGGGAAGAATCGTCTTGCAGCTTAAGAGAGCATTTAAGCGGAACGACAAGCCACAATTACTAGCTGCTGTCAAATTTATCGCGCATCTCGTCAACCAACAAGTGGCTCATGAGATTATAGCATTGGAATTGCTTACTGTGCTGCTGGAGAACCCTACCGATGACAGTGTTGAAGTTGCTGTTGGCTTTGTCACAGAGTGTGGATCGATACTGCAAGACCTTTCACCTAAAGGGCTCCATGGCATCTTTGAGCGCTTCCGTGGAATTCTTCATGAAGGAGAAATTGACAAACGAGTTCAGTTTTTGATCGAAGGCTTATTTGCATTACGAAAAGCAAAATTTCAGGGGCACCCAGCTGTTCGTCCAGAACTGGACCTTGTTGAGCAAGAAGACCAGTTAACTCATGAGATTTCTCTTCAAGAGGAGATAAATCCAGAAATTACCCTTGATATTTTCAAGCCTGATCCTGATTTCATTGAGAATGAGAAGCGCTATGAAGATGTGAAGAAAACAATTCTTGGTGAAGACTCTGAAGAGGAAGGTTCTGATGCAGCTTCAGATGGTGAGGATGATGATGATGAGGAGGAGGACGAAGATGATGATGATGAGGAGGACGAGCAGGAAATGAAAATAAGAGATGAGACGGAGACAAATCTTGTGAATCTTCGAAGGACAATATATCTGACTATCATGTCCAGTGTAGATTTTGAGGAAGCAGGTCATAAGCTCCTTAAGATTAAGCTTGAGCCAGGCCAAGAGATGGAGCTATGTGTCATGCTTTTGGAATGTTGCAGTCAGGAGAGAACCTACCTCCGCTATTATGGTCTCTTGGGACAGCGGTTCTGCATGATCAACAGAATCCACCAGGAAAATTTTGAGAAATGTTTTGTCCAGCAGTATTCAATGATTCACCGGCTTGAAACAAACAAGCTACGTAATGTAGCTAAGTTTTTTGCGCATTTACTCGGAACAGATGCCCTTCCGTGGCATTGCTTAGCCTATATACGGTTAACTGAAGAGGACACCACTTCTTCTTCTCGTATTTTTATCAAGATCCTTTTCCAGGAATTGTCAGAGCATCTTGGTATCCGTCTGCTTAATGAGCGGTTGACTGATCCTGCAATGCAAGACTCTTACGAATCTATTTTCCCAAGGGATAATCCCAAGAACACCCGATTTGCTATTAACTTTTTCACATCCATTGGGCTTGGCGGCATCACTGAGAACCTGCGTGAGTATTTGAAGCACATGCCACGCCTTATTATGCAACAACAAAAAGCAGCATCAGATGAAGAATCCGACAGTTCTGGTTCATCGGATTCGGACTCAGGGTCGGCTAGTTCTGAGTCAGAATCAGAATCAGAATCAGAATCATCAAGCTCTGAAAGTGAAAGTGATCGCGATGAAAGACGCAAGAAGAAACGTAGGAGGAGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001931 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004725 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005938 GO:0006464 GO:0006469 GO:0006470 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007154 GO:0007163 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008104 GO:0008150 GO:0008152 GO:0008154 GO:0008289 GO:0009267 GO:0009605 GO:0009628 GO:0009651 GO:0009892 GO:0009987 GO:0009991 GO:0010563 GO:0010605 GO:0014065 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019637 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0023052 GO:0030010 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030041 GO:0030258 GO:0030587 GO:0031152 GO:0031254 GO:0031257 GO:0031268 GO:0031269 GO:0031272 GO:0031279 GO:0031323 GO:0031324 GO:0031344 GO:0031399 GO:0031400 GO:0031667 GO:0031668 GO:0031669 GO:0032153 GO:0032155 GO:0032268 GO:0032269 GO:0032502 GO:0032796 GO:0032879 GO:0033036 GO:0033554 GO:0033673 GO:0034461 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0034613 GO:0034622 GO:0035335 GO:0035556 GO:0036051 GO:0036052 GO:0036211 GO:0040011 GO:0042221 GO:0042325 GO:0042326 GO:0042330 GO:0042578 GO:0042594 GO:0042995 GO:0043086 GO:0043167 GO:0043168 GO:0043170 GO:0043327 GO:0043412 GO:0043549 GO:0043933 GO:0044085 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0045761 GO:0045859 GO:0045936 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048015 GO:0048017 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0050919 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051248 GO:0051258 GO:0051270 GO:0051272 GO:0051285 GO:0051286 GO:0051301 GO:0051338 GO:0051339 GO:0051348 GO:0051641 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0051800 GO:0052866 GO:0060255 GO:0060491 GO:0061640 GO:0065003 GO:0065007 GO:0065009 GO:0070300 GO:0070727 GO:0071496 GO:0071704 GO:0071840 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0090702 GO:0097435 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0106017 GO:0120025 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140096 GO:1901564 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
ko00562 ko01521 ko03050 ko04068 ko04070 ko04071 ko04115 ko04140 ko04150 ko04151 ko04212 ko04218 ko04510 ko04931 ko05161 ko05165 ko05169 ko05200 ko05206 ko05213 ko05214 ko05215 ko05218 ko05222 ko05224 ko05225 ko05230 map00562 map01521 map03050 map04068 map04070 map04071 map04115 map04140 map04150 map04151 map04212 map04218 map04510 map04931 map05161 map05165 map05169 map05200 map05206 map05213 map05214 map05215 map05218 map05222 map05224 map05225 map05230
Pfam Domains
Protein Families
DSPc

Protein Analysis

776

Amino Acids

90.08

Weight (kDa)

5.44

Isoelectric Point (pI)

58.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF4G PF02854 228 - 409 8.2e-14 MIF4G domain
MA3 PF02847 519 - 625 8.4e-31 MA3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19420
fragaria_vesca FvH4_2g05490 FvH4_2g05490
malus_domestica MD00G1045500.v1.1 MD05G1087100.v1.1
prunus_persica Prupe.8G131700_v2.0.a1
pyrus_communis pycom05g08640 pycom10g08170
rosa_chinensis RchiOBHm_Chr6g0255761
rosa_laevigata RLG00000014815
rosa_roxburghii Rroxscaffold_7G00209690
rosa_rugosa Rorug05G0561200 Rorug05G0561300
rosa_samantha Rh6AG079200 Rh6BG072300 Rh6CG070500 Rh6DG067700
rosa_wichuraiana Rw6G006830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1582
AatII GACGTC 1 cut(s) 834
Acc16I TGCGCA 1 cut(s) 1848
Acc36I ACCTGC 2 cut(s) 1598, 2127
AccB1I GGYRCC 1 cut(s) 1239
AccB7I CCANNNNNTGG 2 cut(s) 994, 1651
AccBSI CCGCTC 1 cut(s) 1996
AccII CGCG 2 cut(s) 974, 2292
AciI CCGC 7 cut(s) 274, 596, 931, 1705, 1729, 1996, 2103
AclWI GGATC 8 cut(s) 32, 45, 93, 783, 1102, 1349, 1939, 2000
AcsI RAATTY 7 cut(s) 505, 565, 744, 965, 1155, 1229, 1762
AcuI CTGAAG 5 cut(s) 260, 345, 1431, 1443, 1926
AcyI GRCGYC 1 cut(s) 831
AdeI CACNNNGTG 1 cut(s) 1089
AfeI AGCGCT 2 cut(s) 1145, 1382
AfiI CCNNNNNNNGG 6 cut(s) 878, 994, 1651, 1875, 1896, 1954
AflII CTTAAG 2 cut(s) 914, 1622
AgsI TTSAA 7 cut(s) 510, 1064, 1310, 1348, 1794, 1811, 2134
AhdI GACNNNNNGTC 1 cut(s) 2221
AjiI CACGTC 1 cut(s) 317
AjnI CCWGG 4 cut(s) 817, 1639, 1755, 1953
AjuI GAANNNNNNNTTGG 2 cut(s) 1056, 1088
Alw26I GTCTC 4 cut(s) 782, 1526, 1532, 1721
AlwI GGATC 8 cut(s) 32, 45, 93, 783, 1102, 1349, 1939, 2000
Aor51HI AGCGCT 2 cut(s) 1145, 1382
AoxI GGCC 2 cut(s) 609, 1642
ApeKI GCWGC 6 cut(s) 716, 910, 956, 1033, 1442, 2171
ApoI RAATTY 7 cut(s) 505, 565, 744, 965, 1155, 1229, 1762
AspLEI GCGC 5 cut(s) 333, 976, 1146, 1383, 1849
AspS9I GGNCC 2 cut(s) 610, 1264
AsuC2I CCSGG 1 cut(s) 416
AsuII TTCGAA 1 cut(s) 1558
AvaII GGWCC 1 cut(s) 1264
AxyI CCTNAGG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 1242
BamHI GGATCC 1 cut(s) 37
BanI GGYRCC 1 cut(s) 1239
BanII GRGCYC 1 cut(s) 1128
BbsI GAAGAC 4 cut(s) 132, 480, 1287, 1422
BbvI GCAGC 6 cut(s) 703, 922, 943, 1020, 1454, 2183
BccI CCATC 6 cut(s) 281, 383, 419, 802, 1445, 1645
BciT130I CCWGG 4 cut(s) 819, 1641, 1757, 1955
BciVI GTATCC 1 cut(s) 1988
BclI TGATCA 1 cut(s) 1740
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 4 cut(s) 782, 1526, 1532, 1721
BfaI CTAG 2 cut(s) 953, 2229
BfoI RGCGCY 2 cut(s) 1147, 1384
BfrI CTTAAG 2 cut(s) 914, 1622
BfuAI ACCTGC 2 cut(s) 1598, 2127
BfuI GTATCC 1 cut(s) 1988
BisI GCNGC 7 cut(s) 717, 911, 957, 1034, 1443, 2104, 2172
BlpI GCTNAGC 1 cut(s) 1885
BlsI GCNGC 7 cut(s) 718, 912, 958, 1035, 1444, 2105, 2173
Bme1390I CCNGG 5 cut(s) 416, 819, 1641, 1757, 1955
Bme18I GGWCC 1 cut(s) 1264
BmeRI GACNNNNNGTC 1 cut(s) 2221
BmgBI CACGTC 1 cut(s) 317
BmgT120I GGNCC 2 cut(s) 610, 1264
BmiI GGNNCC 4 cut(s) 20, 39, 1127, 1241
BmrFI CCNGG 5 cut(s) 416, 819, 1641, 1757, 1955
BmrI ACTGGG 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 4 cut(s) 132, 480, 1287, 1422
BpmI CTGGAG 2 cut(s) 801, 1058
Bpu1102I GCTNAGC 1 cut(s) 1885
Bpu14I TTCGAA 1 cut(s) 1558
BpuEI CTTGAG 3 cut(s) 501, 521, 1655
BpuMI CCSGG 1 cut(s) 416
Bsa29I ATCGAT 1 cut(s) 1097
BsaAI YACGTR 1 cut(s) 1826
BsaBI GATNNNNATC 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 831
BsaI GGTCTC 2 cut(s) 782, 1721
BsaJI CCNNGG 4 cut(s) 1129, 1150, 1874, 2042
Bsc4I CCNNNNNNNGG 6 cut(s) 878, 994, 1651, 1875, 1896, 1954
Bse118I RCCGGY 1 cut(s) 1803
Bse1I ACTGG 4 cut(s) 42, 1266, 1285, 1587
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 3 cut(s) 530, 1879, 2019
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 4 cut(s) 819, 1641, 1757, 1955
BseCI ATCGAT 1 cut(s) 1097
BseDI CCNNGG 4 cut(s) 1129, 1150, 1874, 2042
BseGI GGATG 7 cut(s) 292, 343, 424, 501, 676, 1465, 2087
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 6 cut(s) 878, 994, 1651, 1875, 1896, 1954
BseMI GCAATG 3 cut(s) 530, 1879, 2019
BseMII CTCAG 6 cut(s) 318, 471, 864, 2103, 2226, 2232
BseNI ACTGG 4 cut(s) 42, 1266, 1285, 1587
BseRI GAGGAG 4 cut(s) 1328, 1487, 1490, 1514
BseSI GKGCMC 1 cut(s) 1242
BseXI GCAGC 6 cut(s) 703, 922, 943, 1020, 1454, 2183
BseYI CCCAGC 1 cut(s) 1243
Bsh1236I CGCG 2 cut(s) 974, 2292
BshFI GGCC 2 cut(s) 611, 1644
BshNI GGYRCC 1 cut(s) 1239
BshVI ATCGAT 1 cut(s) 1097
BsiSI CCGG 2 cut(s) 415, 1804
BslFI GGGAC 2 cut(s) 317, 1737
BslI CCNNNNNNNGG 6 cut(s) 878, 994, 1651, 1875, 1896, 1954
BsmAI GTCTC 4 cut(s) 782, 1526, 1532, 1721
BsmBI CGTCTC 1 cut(s) 1526
BsmFI GGGAC 2 cut(s) 317, 1737
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 2 cut(s) 611, 1644
Bso31I GGTCTC 2 cut(s) 782, 1721
Bsp119I TTCGAA 1 cut(s) 1558
Bsp1286I GDGCHC 2 cut(s) 1128, 1242
Bsp1720I GCTNAGC 1 cut(s) 1885
Bsp19I CCATGG 1 cut(s) 1129
Bsp68I TCGCGA 1 cut(s) 2292
BspACI CCGC 7 cut(s) 274, 596, 931, 1705, 1729, 1996, 2103
BspANI GGCC 2 cut(s) 611, 1644
BspCNI CTCAG 6 cut(s) 319, 472, 865, 2104, 2227, 2231
BspDI ATCGAT 1 cut(s) 1097
BspFNI CGCG 2 cut(s) 974, 2292
BspHI TCATGA 3 cut(s) 1000, 1162, 1294
BspLI GGNNCC 4 cut(s) 20, 39, 1127, 1241
BspMI ACCTGC 2 cut(s) 1598, 2127
BspPI GGATC 8 cut(s) 32, 45, 93, 783, 1102, 1349, 1939, 2000
BspQI GCTCTTC 1 cut(s) 342
BspT104I TTCGAA 1 cut(s) 1558
BspT107I GGYRCC 1 cut(s) 1239
BspTI CTTAAG 2 cut(s) 914, 1622
BspTNI GGTCTC 2 cut(s) 782, 1721
BsrBI CCGCTC 1 cut(s) 1996
BsrDI GCAATG 3 cut(s) 530, 1879, 2019
BsrFI RCCGGY 1 cut(s) 1803
BsrI ACTGG 4 cut(s) 42, 1266, 1285, 1587
BssAI RCCGGY 1 cut(s) 1803
BssECI CCNNGG 4 cut(s) 1129, 1150, 1874, 2042
BssNI GRCGYC 1 cut(s) 831
BssT1I CCWWGG 2 cut(s) 1129, 2042
Bst2UI CCWGG 4 cut(s) 819, 1641, 1757, 1955
Bst4CI ACNGT 5 cut(s) 525, 1030, 1058, 1899, 2195
Bst6I CTCTTC 6 cut(s) 236, 342, 766, 1311, 1419, 1902
BstACI GRCGYC 1 cut(s) 831
BstAFI CTTAAG 2 cut(s) 914, 1622
BstBAI YACGTR 1 cut(s) 1826
BstBI TTCGAA 1 cut(s) 1558
BstC8I GCNNGC 1 cut(s) 841
BstDEI CTNAG 9 cut(s) 222, 327, 480, 873, 1836, 1885, 2112, 2218, 2235
BstDSI CCRYGG 3 cut(s) 1129, 1150, 1874
BstENI CCTNNNNNAGG 1 cut(s) 1952
BstF5I GGATG 7 cut(s) 292, 343, 424, 501, 676, 1465, 2087
BstFNI CGCG 2 cut(s) 974, 2292
BstH2I RGCGCY 2 cut(s) 1147, 1384
BstHHI GCGC 5 cut(s) 333, 976, 1146, 1383, 1849
BstMAI GTCTC 4 cut(s) 782, 1526, 1532, 1721
BstMWI GCNNNNNNNGC 3 cut(s) 403, 1030, 1993
BstNI CCWGG 4 cut(s) 819, 1641, 1757, 1955
BstNSI RCATGY 1 cut(s) 2143
BstSCI CCNGG 5 cut(s) 414, 817, 1639, 1755, 1953
BstSLI GKGCMC 1 cut(s) 1242
BstSNI TACGTA 1 cut(s) 1826
BstUI CGCG 2 cut(s) 974, 2292
BstV1I GCAGC 6 cut(s) 703, 922, 943, 1020, 1454, 2183
BstV2I GAAGAC 4 cut(s) 132, 480, 1287, 1422
BstX2I RGATCY 2 cut(s) 37, 1944
BstYI RGATCY 2 cut(s) 37, 1944
Bsu15I ATCGAT 1 cut(s) 1097
Bsu36I CCTNAGG 1 cut(s) 873
BsuI GTATCC 1 cut(s) 1988
BsuRI GGCC 2 cut(s) 611, 1644
BsuTUI ATCGAT 1 cut(s) 1097
BtgI CCRYGG 3 cut(s) 1129, 1150, 1874
BtgZI GCGATG 1 cut(s) 2307
BtrI CACGTC 1 cut(s) 317
BtsCI GGATG 7 cut(s) 292, 343, 424, 501, 676, 1465, 2087
BtsIMutI CAGTG 5 cut(s) 49, 477, 1063, 1594, 2109
BtuMI TCGCGA 1 cut(s) 2292
BveI ACCTGC 2 cut(s) 1598, 2127
Cac8I GCNNGC 1 cut(s) 841
CciI TCATGA 3 cut(s) 1000, 1162, 1294
CfoI GCGC 5 cut(s) 333, 976, 1146, 1383, 1849
Cfr10I RCCGGY 1 cut(s) 1803
Cfr13I GGNCC 2 cut(s) 610, 1264
ClaI ATCGAT 1 cut(s) 1097
CseI GACGC 2 cut(s) 634, 2310
CviAII CATG 9 cut(s) 666, 1001, 1130, 1163, 1295, 1582, 1666, 1738, 2140
DdeI CTNAG 9 cut(s) 222, 327, 480, 873, 1836, 1885, 2112, 2218, 2235
DraIII CACNNNGTG 1 cut(s) 1089
DrdI GACNNNNNNGTC 1 cut(s) 1582
DriI GACNNNNNGTC 1 cut(s) 2221
DseDI GACNNNNNNGTC 1 cut(s) 1582
Eam1104I CTCTTC 6 cut(s) 236, 342, 766, 1311, 1419, 1902
Eam1105I GACNNNNNGTC 1 cut(s) 2221
EarI CTCTTC 6 cut(s) 236, 342, 766, 1311, 1419, 1902
EciI GGCGGA 1 cut(s) 585
Eco105I TACGTA 1 cut(s) 1826
Eco130I CCWWGG 2 cut(s) 1129, 2042
Eco24I GRGCYC 1 cut(s) 1128
Eco31I GGTCTC 2 cut(s) 782, 1721
Eco47I GGWCC 1 cut(s) 1264
Eco47III AGCGCT 2 cut(s) 1145, 1382
Eco57I CTGAAG 5 cut(s) 260, 345, 1431, 1443, 1926
Eco81I CCTNAGG 1 cut(s) 873
EcoNI CCTNNNNNAGG 1 cut(s) 1952
EcoRI GAATTC 2 cut(s) 505, 1155
EcoRII CCWGG 4 cut(s) 817, 1639, 1755, 1953
EcoT14I CCWWGG 2 cut(s) 1129, 2042
EcoT38I GRGCYC 1 cut(s) 1128
ErhI CCWWGG 2 cut(s) 1129, 2042
Esp3I CGTCTC 1 cut(s) 1526
FaeI CATG 9 cut(s) 669, 1004, 1133, 1166, 1298, 1585, 1669, 1741, 2143
FaqI GGGAC 2 cut(s) 317, 1737
FatI CATG 9 cut(s) 665, 1000, 1129, 1162, 1294, 1581, 1665, 1737, 2139
FauI CCCGC 1 cut(s) 267
FbaI TGATCA 1 cut(s) 1740
Fnu4HI GCNGC 7 cut(s) 717, 911, 957, 1034, 1443, 2104, 2172
FokI GGATG 7 cut(s) 299, 350, 431, 508, 683, 1472, 2074
FriOI GRGCYC 1 cut(s) 1128
Fsp4HI GCNGC 7 cut(s) 717, 911, 957, 1034, 1443, 2104, 2172
FspBI CTAG 2 cut(s) 953, 2229
FspI TGCGCA 1 cut(s) 1848
GlaI GCGC 5 cut(s) 332, 975, 1145, 1382, 1848
GluI GCNGC 7 cut(s) 717, 911, 957, 1034, 1443, 2104, 2172
GsaI CCCAGC 1 cut(s) 1247
GsuI CTGGAG 2 cut(s) 801, 1058
HaeII RGCGCY 2 cut(s) 1147, 1384
HaeIII GGCC 2 cut(s) 611, 1644
HapII CCGG 2 cut(s) 415, 1804
HgaI GACGC 2 cut(s) 634, 2310
HhaI GCGC 5 cut(s) 333, 976, 1146, 1383, 1849
Hin1I GRCGYC 1 cut(s) 831
Hin1II CATG 9 cut(s) 669, 1004, 1133, 1166, 1298, 1585, 1669, 1741, 2143
Hin6I GCGC 5 cut(s) 331, 974, 1144, 1381, 1847
HinP1I GCGC 5 cut(s) 331, 974, 1144, 1381, 1847
HincII GTYRAC 6 cut(s) 528, 859, 985, 1290, 1902, 2001
HindII GTYRAC 6 cut(s) 528, 859, 985, 1290, 1902, 2001
HindIII AAGCTT 1 cut(s) 1631
HpaI GTTAAC 3 cut(s) 859, 1290, 1902
HpaII CCGG 2 cut(s) 415, 1804
Hpy166II GTNNAC 9 cut(s) 263, 528, 703, 859, 884, 985, 1290, 1902, 2001
Hpy8I GTNNAC 9 cut(s) 263, 528, 703, 859, 884, 985, 1290, 1902, 2001
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 8 cut(s) 51, 144, 264, 1160, 1196, 1424, 1554, 1880
HpyCH4III ACNGT 5 cut(s) 525, 1030, 1058, 1899, 2195
HpyCH4IV ACGT 6 cut(s) 28, 178, 316, 831, 1825, 2314
HpyF10VI GCNNNNNNNGC 3 cut(s) 403, 1030, 1993
HpyF3I CTNAG 9 cut(s) 222, 327, 480, 873, 1836, 1885, 2112, 2218, 2235
HpySE526I ACGT 6 cut(s) 28, 178, 316, 831, 1825, 2314
Hsp92I GRCGYC 1 cut(s) 831
Hsp92II CATG 9 cut(s) 669, 1004, 1133, 1166, 1298, 1585, 1669, 1741, 2143
HspAI GCGC 5 cut(s) 331, 974, 1144, 1381, 1847
Ksp22I TGATCA 1 cut(s) 1740
KspAI GTTAAC 3 cut(s) 859, 1290, 1902
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 3 cut(s) 1131, 1623, 1654
Lsp1109I GCAGC 6 cut(s) 703, 922, 943, 1020, 1454, 2183
MaeI CTAG 2 cut(s) 953, 2229
MaeII ACGT 6 cut(s) 28, 178, 316, 831, 1825, 2314
MaeIII GTNAC 3 cut(s) 317, 801, 1081
MbiI CCGCTC 1 cut(s) 1996
MflI RGATCY 2 cut(s) 37, 1944
MhlI GDGCHC 2 cut(s) 1128, 1242
MlyI GAGTC 4 cut(s) 1412, 2015, 2210, 2246
MmeI TCCRAC 1 cut(s) 2214
MspA1I CMGCKG 2 cut(s) 1247, 1729
MspCI CTTAAG 2 cut(s) 914, 1622
MspI CCGG 2 cut(s) 415, 1804
MspR9I CCNGG 5 cut(s) 416, 819, 1641, 1757, 1955
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 4 cut(s) 819, 1641, 1757, 1955
MvnI CGCG 2 cut(s) 974, 2292
MwoI GCNNNNNNNGC 3 cut(s) 403, 1030, 1993
NciI CCSGG 1 cut(s) 416
NcoI CCATGG 1 cut(s) 1129
NlaIII CATG 9 cut(s) 669, 1004, 1133, 1166, 1298, 1585, 1669, 1741, 2143
NlaIV GGNNCC 4 cut(s) 20, 39, 1127, 1241
NmuCI GTSAC 3 cut(s) 317, 801, 1081
NruI TCGCGA 1 cut(s) 2292
NsbI TGCGCA 1 cut(s) 1848
NspI RCATGY 1 cut(s) 2143
NspV TTCGAA 1 cut(s) 1558
PagI TCATGA 3 cut(s) 1000, 1162, 1294
PciSI GCTCTTC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 978
PctI GAATGC 1 cut(s) 310
PflMI CCANNNNNTGG 2 cut(s) 994, 1651
PfoI TCCNGGA 2 cut(s) 817, 1953
PkrI GCNGC 7 cut(s) 718, 912, 958, 1035, 1444, 2105, 2173
PleI GAGTC 4 cut(s) 1412, 2015, 2210, 2245
PpsI GAGTC 4 cut(s) 1412, 2015, 2210, 2245
Ppu21I YACGTR 1 cut(s) 1826
Psp6I CCWGG 4 cut(s) 817, 1639, 1755, 1953
PspFI CCCAGC 1 cut(s) 1243
PspGI CCWGG 4 cut(s) 817, 1639, 1755, 1953
PspN4I GGNNCC 4 cut(s) 20, 39, 1127, 1241
PspPI GGNCC 2 cut(s) 610, 1264
PsuI RGATCY 2 cut(s) 37, 1944
PvuII CAGCTG 1 cut(s) 1247
RruI TCGCGA 1 cut(s) 2292
SapI GCTCTTC 1 cut(s) 342
SatI GCNGC 7 cut(s) 717, 911, 957, 1034, 1443, 2104, 2172
Sau96I GGNCC 2 cut(s) 610, 1264
SchI GAGTC 4 cut(s) 1412, 2015, 2210, 2246
ScrFI CCNGG 5 cut(s) 416, 819, 1641, 1757, 1955
SduI GDGCHC 2 cut(s) 1128, 1242
SfuI TTCGAA 1 cut(s) 1558
SinI GGWCC 1 cut(s) 1264
SmlI CTYRAG 5 cut(s) 500, 516, 914, 1622, 1634
SmoI CTYRAG 5 cut(s) 500, 516, 914, 1622, 1634
SnaBI TACGTA 1 cut(s) 1826
SsiI CCGC 7 cut(s) 274, 596, 931, 1705, 1729, 1996, 2103
SspMI CTAG 2 cut(s) 953, 2229
StyD4I CCNGG 5 cut(s) 414, 817, 1639, 1755, 1953
StyI CCWWGG 2 cut(s) 1129, 2042
TaaI ACNGT 5 cut(s) 525, 1030, 1058, 1899, 2195
TaiI ACGT 6 cut(s) 31, 181, 319, 834, 1828, 2317
TaqI TCGA 7 cut(s) 88, 630, 648, 787, 1097, 1200, 1558
TauI GCSGC 1 cut(s) 2106
TscAI CASTG 5 cut(s) 49, 484, 1063, 1594, 2116
TseFI GTSAC 3 cut(s) 317, 801, 1081
TseI GCWGC 6 cut(s) 716, 910, 956, 1033, 1442, 2171
Tsp45I GTSAC 3 cut(s) 317, 801, 1081
TspGWI ACGGA 5 cut(s) 382, 1139, 1550, 1863, 1970
TspRI CASTG 5 cut(s) 49, 484, 1063, 1594, 2116
Van91I CCANNNNNTGG 2 cut(s) 994, 1651
Vha464I CTTAAG 2 cut(s) 914, 1622
VpaK11BI GGWCC 1 cut(s) 1264
XagI CCTNNNNNAGG 1 cut(s) 1952
XapI RAATTY 7 cut(s) 505, 565, 744, 965, 1155, 1229, 1762
XceI RCATGY 1 cut(s) 2143
XspI CTAG 2 cut(s) 953, 2229
ZraI GACGTC 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.