Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0011220

Physical Location & Seq

Genomic Coordinates Reverse (-)

18893673 .. 18897673

Gene Structure 4001 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1088500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGACCTGGTGGAGGGTTGCCTTCAATTTTCTTCTTCTTCTTCTTCCTACAACATTTGCTCTCAACTCAGATGGGGTCCTTCTGCTTTCCTTCAAATACTCAATCCTCAGCGATCCGCTCTCAGTCCTCGACTCCTGGAACTACGAAGACGAAACCCCATGTTCCTGGACTGGCGTCACGTGCACCCAGCTGGGAAGCCCAGGTACCCCAGACATGTTCAGAGTCACCAGCTTAGTCCTCCCCAACAGCCAGCTGCTGGGTTCAATCTCACCCGAGCTGGGTTCACTCCAACACCTTCAAAGACTCGATCTTTCCAACAATTTCTTCAATGGGTCTCTGCCTGTCGCCCTCTTCAATGCCTCGGAGCTTCAGGTCCTCTCTCTCTCCAGCAATGTGATCTCCGGCGACCTGCCGGATCAGTTGGTCTCCGGCCTCAAAGGCCTCCAGTTTCTCAACGTCTCCGACAATGCATTGGCCGGAAGAATCCCAGAAATTCTCACTTCTTTACAAAACCTCACTGTAATTTCTCTAAGGAGTAACTACTTTTCTGGGAATCTACCCACTGGTTTTAAGTCCATTGAGGTTTTGGATCTCTCCTCCAACCTCCTCAACGGGACTCTGCCTCTCGACTTCGGCGGTGAAAACCTGCGCTACTTGAACCTCTCCTACAACAAAATCTCTGGGAAATTTCCAGTTGGTTTCGCGAAAAGGGTTTCGGAGAACTCGACTATTGATCTCTCCTTCAACAATTTGACCGGACCGATACCGGACTCGCGGGCTCTGCTCAATCAGAAAACAGAGCAGTTTGCTGGAAACAGTGAGCTTTGTGGGAAGCCATTGAAGACCCTCTGTCCCATTCCTTCTACTCTGTCCACACCGCCGAATATGACAACTAGTACCAGTTCTTCTCCGGCGATTGCTGCCATTCCCAGAACGTTTGATACCCGCCCGGAAACGAACACTTCTGGGGGAGCAAATGGGAATCGGAATCAGTCGCAGAGCGGATTGAAACCTGGCACGATCGCCGGCATTGCCGTCGGAGACTTGGCCGGAATAGCTGTACTAGGATTGGTGATTCTCTATGTCTACCAAGTTAGGAAGAGAAAAAGTCTCAAGTCAAAGGGTGGTAGTGCCAAAGACCTGGAAAATAAGCCAGAAATTGTCACAAAACTAGACCCAGATGAAAAAGCAAAATCCGCAGCATGGTCATGCCTAACAATCAAAGGGGAAGAAACATCAGAGGCAACAAGCTCAGACAGTGACCATGATGATAAAAATGAACAAATGGGTTGTCTGCAGAATCAGAACGGGGAGTCTCAGAAAAGTGGCATCTTAGTGACCATAGAAGGCGGGGACCAACTGGAGCTGGAACTCGAGACACTGCTGAAAGCATCAGCTTATATATTGGGAGCCAGCGGCCCAAGCATTGTGTACAAGGCAGTGCTGGAGAACAAGACTGAGCTTGCAGTGAGGAGGATAGGAGAGTGTAGGGTTTTGAAGATGAGGGATTTCGAGAGCCTAGTGCGGGCCATTGCGAAGGTGAGGCATCCGAATCTGGTTAGGGTTCGTGGGTTTTACTGGGGAGACGATGAGAAGCTTGTTATCTATGACTATGTCTCCAATGGCAGCCTTGCCACCACCACCAACAGAAGAGCTGGGTTGTCGCCGTGTCATCTACCTCTCCAAACTCGGATGAAGATCGCAAGAGGGGTTGCGAAGGGGCTGGCTTACATCCATGAGAAGAAACATGTTCACGGAAACATCAAACCTAGCAACATCCTTTTAAATCCTGATATGGAGCCCATAATTAGTGATTTCGGCCTTGATAAGCTGATGTTAGGCAACATGATTAGTCATAAAGCTAGCGGGTCAGCTCGGGGGTACTTCGGAAGCCTAAAATCCACGGCGACTTGTGAAGGTACGCATGACGCGCTCCCAATAGGCAGCAGCCCCGTGGCAATGCCATCCGCGGGGAATGCATCTTTGCCTTATCAAGCGCCGGAGTCCCTAAAGAACTTGAAGCCAAACCCCAAGTGGGATGTGTATTCTTTTGGTATAGTTTTGCTTGAGCTTCTCACGGGAAGGATACCTTCGGAACGAGAATTGGTTGCCCAATGGACACCGGGTACGCGGACGGAGGAGAAAATTCGAATTTTAATAATGGTTGACTTGGCGATTAGGGCTGAGGTGGAGGGCAGGGAAGATGCCTTGCTGGCTTGTTTGAAATTAGGGTTTAGTTGTGCTAGTTTTAGTCCACAAAAAAGACCAACCATGAAAGAAGCCTTCCAAACCCTAGACAAAAGTACTCGTCCTTGTTCCACATCTTCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0007154 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010029 GO:0010030 GO:0010035 GO:0010646 GO:0010647 GO:0016020 GO:0023051 GO:0023056 GO:0031668 GO:0033554 GO:0042221 GO:0042631 GO:0044464 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071944 GO:0104004 GO:1900140 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1905957 GO:1905959 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

778

Amino Acids

84.17

Weight (kDa)

7.86

Isoelectric Point (pI)

35.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	21 - 62	3.6e-13	Leucine rich repeat N-terminal domain
Pkinase	PF00069	469 - 612	9e-18	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	476 - 612	1.4e-18	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	658 - 763	3e-06	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	660 - 763	2.2e-08	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011220)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15300 AT4G34220
fragaria_vesca	FvH4_2g05270
malus_domestica	MD05G1088500.v1.1 MD10G1096400.v1.1
prunus_persica	Prupe.8G134500_v2.0.a1
pyrus_communis	pycom05g08790 pycom10g08270
rosa_chinensis	RchiOBHm_Chr6g0255401
rosa_laevigata	RLG00000014841
rosa_multiflora	Rmu_co8383769.1_g000001 Rmu_sc0002360.1_g000015
rosa_roxburghii	Rroxscaffold_7G00210020
rosa_rugosa	Rorug05G0558100.1 Rorug05G0558200
rosa_samantha	Rh6AG075300 Rh6BG068400 Rh6CG068000 Rh6DG064700
rosa_wichuraiana	Rw6G006620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	417, 654
Acc65I	GGTACC	1 cut(s)	203
AccB1I	GGYRCC	1 cut(s)	203
AccB7I	CCANNNNNTGG	1 cut(s)	256
AccBSI	CCGCTC	2 cut(s)	118, 1002
AccI	GTMKAC	1 cut(s)	1086
AccII	CGCG	5 cut(s)	704, 775, 1931, 1970, 2131
AclI	AACGTT	1 cut(s)	935
AclWI	GGATC	3 cut(s)	107, 423, 597
AcoI	YGGCCR	2 cut(s)	474, 1047
AcsI	RAATTY	4 cut(s)	492, 686, 2145, 2151
AcuI	CTGAAG	1 cut(s)	353
AcvI	CACGTG	1 cut(s)	180
AcyI	GRCGYC	1 cut(s)	174
AfaI	GTAC	8 cut(s)	205, 898, 1062, 1433, 1883, 1921, 2128, 2305
AfiI	CCNNNNNNNGG	7 cut(s)	12, 256, 278, 1203, 1525, 1970, 2035
AflIII	ACRYGT	2 cut(s)	213, 1747
AhdI	GACNNNNNGTC	1 cut(s)	849
AhlI	ACTAGT	1 cut(s)	893
AjnI	CCWGG	6 cut(s)	5, 134, 164, 199, 1012, 1140
AjuI	GAANNNNNNNTTGG	2 cut(s)	308, 340
Alw21I	GWGCWC	1 cut(s)	185
Alw26I	GTCTC	9 cut(s)	339, 430, 463, 1035, 1115, 1320, 1370, 1578, 1621
Alw44I	GTGCAC	1 cut(s)	181
AlwI	GGATC	3 cut(s)	107, 423, 597
AlwNI	CAGNNNCTG	1 cut(s)	256
Ama87I	CYCGRG	3 cut(s)	272, 1373, 1875
AoxI	GGCC	7 cut(s)	430, 439, 474, 1047, 1417, 1527, 1819
ApaLI	GTGCAC	1 cut(s)	181
ApeKI	GCWGC	6 cut(s)	253, 920, 1199, 1626, 1944, 1947
ApoI	RAATTY	4 cut(s)	492, 686, 2145, 2151
ArsI	GACNNNNNNTTYG	2 cut(s)	1276, 1308
Asp718I	GGTACC	1 cut(s)	203
AspLEI	GCGC	3 cut(s)	651, 1933, 1999
AspS9I	GGNCC	6 cut(s)	76, 373, 758, 1354, 1418, 1527
AsuC2I	CCSGG	2 cut(s)	950, 2124
AsuHPI	GGTGA	5 cut(s)	217, 261, 650, 1084, 1552
AsuII	TTCGAA	1 cut(s)	2149
AsuNHI	GCTAGC	1 cut(s)	1862
AvaI	CYCGRG	3 cut(s)	272, 1373, 1875
AvaII	GGWCC	4 cut(s)	76, 373, 758, 1354
BaeGI	GKGCMC	1 cut(s)	185
BanI	GGYRCC	1 cut(s)	203
BanII	GRGCYC	2 cut(s)	781, 1803
BarI	GAAGNNNNNNTAC	4 cut(s)	187, 219, 889, 921
BbrPI	CACGTG	1 cut(s)	180
BbsI	GAAGAC	2 cut(s)	153, 848
Bbv12I	GWGCWC	1 cut(s)	185
BbvCI	CCTCAGC	2 cut(s)	107, 2184
BbvI	GCAGC	6 cut(s)	240, 907, 1211, 1638, 1956, 1959
BccI	CCATC	2 cut(s)	65, 1972
BceAI	ACGGC	3 cut(s)	1019, 1651, 1920
BcgI	CGANNNNNNTGC	2 cut(s)	1018, 1052
BciT130I	CCWGG	6 cut(s)	7, 136, 166, 201, 1014, 1142
BciVI	GTATCC	1 cut(s)	2079
BcnI	CCSGG	2 cut(s)	950, 2124
BcoDI	GTCTC	9 cut(s)	339, 430, 463, 1035, 1115, 1320, 1370, 1578, 1621
BcuI	ACTAGT	1 cut(s)	893
BfaI	CTAG	8 cut(s)	894, 1064, 1172, 1520, 1770, 1863, 2244, 2294
BfmI	CTRYAG	1 cut(s)	1295
BfoI	RGCGCY	1 cut(s)	2000
BfuAI	ACCTGC	2 cut(s)	417, 654
BfuI	GTATCC	1 cut(s)	2079
BglI	GCCNNNNNGGC	2 cut(s)	438, 2213
BisI	GCNGC	7 cut(s)	254, 921, 1200, 1417, 1627, 1945, 1948
BlsI	GCNGC	7 cut(s)	255, 922, 1201, 1418, 1628, 1946, 1949
BmcAI	AGTACT	1 cut(s)	2305
Bme1390I	CCNGG	8 cut(s)	7, 136, 166, 201, 950, 1014, 1142, 2124
Bme18I	GGWCC	4 cut(s)	76, 373, 758, 1354
BmeRI	GACNNNNNGTC	1 cut(s)	849
BmeT110I	CYCGRG	3 cut(s)	272, 1373, 1875
BmgT120I	GGNCC	6 cut(s)	76, 373, 758, 1354, 1418, 1527
BmiI	GGNNCC	5 cut(s)	77, 205, 1355, 1411, 1800
BmrFI	CCNGG	8 cut(s)	7, 136, 166, 201, 950, 1014, 1142, 2124
BmrI	ACTGGG	1 cut(s)	1588
BmsI	GCATC	5 cut(s)	1338, 1400, 1555, 1988, 2194
BmtI	GCTAGC	1 cut(s)	1866
BmuI	ACTGGG	1 cut(s)	1588
BoxI	GACNNNNGTC	1 cut(s)	173
BpiI	GAAGAC	2 cut(s)	153, 848
BpmI	CTGGAG	4 cut(s)	370, 428, 1382, 1466
Bpu10I	CCTNAGC	2 cut(s)	107, 2184
Bpu14I	TTCGAA	1 cut(s)	2149
BpuEI	CTTGAG	2 cut(s)	1097, 2087
BpuMI	CCSGG	2 cut(s)	950, 2124
BsaAI	YACGTR	1 cut(s)	180
BsaBI	GATNNNNATC	1 cut(s)	1697
BsaHI	GRCGYC	1 cut(s)	174
BsaI	GGTCTC	2 cut(s)	339, 430
BsaJI	CCNNGG	5 cut(s)	199, 360, 1902, 1953, 1968
BsaWI	WCCGGW	2 cut(s)	755, 766
BsaXI	ACNNNNNCTCC	2 cut(s)	1601, 1631
Bsc4I	CCNNNNNNNGG	7 cut(s)	12, 256, 278, 1203, 1525, 1970, 2035
Bse118I	RCCGGY	1 cut(s)	1025
Bse1I	ACTGG	7 cut(s)	175, 445, 568, 692, 900, 1365, 1583
Bse3DI	GCAATG	4 cut(s)	397, 1029, 1530, 1965
Bse8I	GATNNNNATC	1 cut(s)	1697
BseBI	CCWGG	6 cut(s)	7, 136, 166, 201, 1014, 1142
BseDI	CCNNGG	5 cut(s)	199, 360, 1902, 1953, 1968
BseGI	GGATG	6 cut(s)	1546, 1698, 1731, 1776, 1964, 2044
BseJI	GATNNNNATC	1 cut(s)	1697
BseLI	CCNNNNNNNGG	7 cut(s)	12, 256, 278, 1203, 1525, 1970, 2035
BseMI	GCAATG	4 cut(s)	397, 1029, 1530, 1965
BseMII	CTCAG	7 cut(s)	81, 121, 135, 1266, 1331, 1449, 2175
BseNI	ACTGG	7 cut(s)	175, 445, 568, 692, 900, 1365, 1583
BseRI	GAGGAG	4 cut(s)	586, 596, 1486, 2153
BseSI	GKGCMC	1 cut(s)	185
BseXI	GCAGC	6 cut(s)	240, 907, 1211, 1638, 1956, 1959
BseYI	CCCAGC	5 cut(s)	186, 190, 256, 277, 1655
Bsh1236I	CGCG	5 cut(s)	704, 775, 1931, 1970, 2131
Bsh1285I	CGRYCG	1 cut(s)	1023
BshFI	GGCC	7 cut(s)	432, 441, 476, 1049, 1419, 1529, 1821
BshNI	GGYRCC	1 cut(s)	203
BsiEI	CGRYCG	1 cut(s)	1023
BsiHKAI	GWGCWC	1 cut(s)	185
BsiHKCI	CYCGRG	3 cut(s)	272, 1373, 1875
BslFI	GGGAC	4 cut(s)	628, 837, 1367, 1990
BslI	CCNNNNNNNGG	7 cut(s)	12, 256, 278, 1203, 1525, 1970, 2035
BsmAI	GTCTC	9 cut(s)	339, 430, 463, 1035, 1115, 1320, 1370, 1578, 1621
BsmBI	CGTCTC	2 cut(s)	463, 1578
BsmFI	GGGAC	4 cut(s)	628, 837, 1367, 1990
BsmI	GAATGC	1 cut(s)	1981
BsnI	GGCC	7 cut(s)	432, 441, 476, 1049, 1419, 1529, 1821
Bso31I	GGTCTC	2 cut(s)	339, 430
BsoBI	CYCGRG	3 cut(s)	272, 1373, 1875
Bsp119I	TTCGAA	1 cut(s)	2149
Bsp1286I	GDGCHC	3 cut(s)	185, 781, 1803
Bsp1407I	TGTACA	1 cut(s)	1431
Bsp143I	GATC	8 cut(s)	112, 307, 396, 415, 589, 733, 1020, 1698
Bsp68I	TCGCGA	1 cut(s)	704
BspANI	GGCC	7 cut(s)	432, 441, 476, 1049, 1419, 1529, 1821
BspCNI	CTCAG	7 cut(s)	80, 120, 134, 1265, 1330, 1450, 2176
BspFNI	CGCG	5 cut(s)	704, 775, 1931, 1970, 2131
BspLI	GGNNCC	5 cut(s)	77, 205, 1355, 1411, 1800
BspMAI	CTGCAG	1 cut(s)	1299
BspMI	ACCTGC	2 cut(s)	417, 654
BspOI	GCTAGC	1 cut(s)	1866
BspPI	GGATC	3 cut(s)	107, 423, 597
BspQI	GCTCTTC	1 cut(s)	1645
BspT104I	TTCGAA	1 cut(s)	2149
BspT107I	GGYRCC	1 cut(s)	203
BspTNI	GGTCTC	2 cut(s)	339, 430
BsrBI	CCGCTC	2 cut(s)	118, 1002
BsrDI	GCAATG	4 cut(s)	397, 1029, 1530, 1965
BsrFI	RCCGGY	1 cut(s)	1025
BsrGI	TGTACA	1 cut(s)	1431
BsrI	ACTGG	7 cut(s)	175, 445, 568, 692, 900, 1365, 1583
BssAI	RCCGGY	1 cut(s)	1025
BssECI	CCNNGG	5 cut(s)	199, 360, 1902, 1953, 1968
BssMI	GATC	8 cut(s)	112, 307, 396, 415, 589, 733, 1020, 1698
BssNI	GRCGYC	1 cut(s)	174
Bst2UI	CCWGG	6 cut(s)	7, 136, 166, 201, 1014, 1142
Bst4CI	ACNGT	3 cut(s)	520, 818, 1259
Bst6I	CTCTTC	3 cut(s)	356, 1094, 1645
BstACI	GRCGYC	1 cut(s)	174
BstAUI	TGTACA	1 cut(s)	1431
BstBAI	YACGTR	1 cut(s)	180
BstBI	TTCGAA	1 cut(s)	2149
BstC8I	GCNNGC	9 cut(s)	251, 777, 1027, 1414, 1464, 1527, 1725, 1864, 2214
BstDSI	CCRYGG	3 cut(s)	1902, 1953, 1968
BstENI	CCTNNNNNAGG	1 cut(s)	10
BstF5I	GGATG	6 cut(s)	1546, 1698, 1731, 1776, 1964, 2044
BstFNI	CGCG	5 cut(s)	704, 775, 1931, 1970, 2131
BstH2I	RGCGCY	1 cut(s)	2000
BstHHI	GCGC	3 cut(s)	651, 1933, 1999
BstKTI	GATC	8 cut(s)	115, 310, 399, 418, 592, 736, 1023, 1701
BstMAI	GTCTC	9 cut(s)	339, 430, 463, 1035, 1115, 1320, 1370, 1578, 1621
BstMBI	GATC	8 cut(s)	112, 307, 396, 415, 589, 733, 1020, 1698
BstMCI	CGRYCG	1 cut(s)	1023
BstNI	CCWGG	6 cut(s)	7, 136, 166, 201, 1014, 1142
BstNSI	RCATGY	2 cut(s)	217, 1751
BstPAI	GACNNNNGTC	1 cut(s)	173
BstSCI	CCNGG	8 cut(s)	5, 134, 164, 199, 948, 1012, 1140, 2122
BstSFI	CTRYAG	1 cut(s)	1295
BstSLI	GKGCMC	1 cut(s)	185
BstUI	CGCG	5 cut(s)	704, 775, 1931, 1970, 2131
BstV1I	GCAGC	6 cut(s)	240, 907, 1211, 1638, 1956, 1959
BstV2I	GAAGAC	2 cut(s)	153, 848
BstX2I	RGATCY	1 cut(s)	589
BstXI	CCANNNNNNTGG	2 cut(s)	165, 1141
BstYI	RGATCY	1 cut(s)	589
BsuI	GTATCC	1 cut(s)	2079
BsuRI	GGCC	7 cut(s)	432, 441, 476, 1049, 1419, 1529, 1821
BtgI	CCRYGG	3 cut(s)	1902, 1953, 1968
BtsCI	GGATG	6 cut(s)	1546, 1698, 1731, 1776, 1964, 2044
BtsI	GCAGTG	3 cut(s)	1379, 1446, 1473
BtsIMutI	CAGTG	7 cut(s)	516, 561, 823, 1264, 1379, 1446, 1473
BtuMI	TCGCGA	1 cut(s)	704
BveI	ACCTGC	2 cut(s)	417, 654
Cac8I	GCNNGC	9 cut(s)	251, 777, 1027, 1414, 1464, 1527, 1725, 1864, 2214
CaiI	CAGNNNCTG	1 cut(s)	256
CfoI	GCGC	3 cut(s)	651, 1933, 1999
Cfr10I	RCCGGY	1 cut(s)	1025
Cfr13I	GGNCC	6 cut(s)	76, 373, 758, 1354, 1418, 1527
Cfr42I	CCGCGG	1 cut(s)	1971
CpoI	CGGWCCG	1 cut(s)	758
CseI	GACGC	2 cut(s)	163, 1937
CsiI	ACCWGGT	1 cut(s)	5
Csp6I	GTAC	8 cut(s)	204, 897, 1061, 1432, 1882, 1920, 2127, 2304
CspI	CGGWCCG	1 cut(s)	758
CviQI	GTAC	8 cut(s)	204, 897, 1061, 1432, 1882, 1920, 2127, 2304
DpnI	GATC	8 cut(s)	114, 309, 398, 417, 591, 735, 1022, 1700
DpnII	GATC	8 cut(s)	112, 307, 396, 415, 589, 733, 1020, 1698
DraI	TTTAAA	1 cut(s)	1785
DriI	GACNNNNNGTC	1 cut(s)	849
EaeI	YGGCCR	2 cut(s)	474, 1047
Eam1104I	CTCTTC	3 cut(s)	356, 1094, 1645
Eam1105I	GACNNNNNGTC	1 cut(s)	849
EarI	CTCTTC	3 cut(s)	356, 1094, 1645
Eco147I	AGGCCT	1 cut(s)	441
Eco24I	GRGCYC	2 cut(s)	781, 1803
Eco31I	GGTCTC	2 cut(s)	339, 430
Eco47I	GGWCC	4 cut(s)	76, 373, 758, 1354
Eco57I	CTGAAG	1 cut(s)	353
Eco72I	CACGTG	1 cut(s)	180
Eco88I	CYCGRG	3 cut(s)	272, 1373, 1875
EcoNI	CCTNNNNNAGG	1 cut(s)	10
EcoO109I	RGGNCCY	2 cut(s)	76, 373
EcoRII	CCWGG	6 cut(s)	5, 134, 164, 199, 1012, 1140
EcoT22I	ATGCAT	2 cut(s)	472, 1981
EcoT38I	GRGCYC	2 cut(s)	781, 1803
Esp3I	CGTCTC	2 cut(s)	463, 1578
FalI	AAGNNNNNCTT	2 cut(s)	2074, 2106
FaqI	GGGAC	4 cut(s)	628, 837, 1367, 1990
FauI	CCCGC	6 cut(s)	768, 953, 1343, 1518, 1859, 1963
FblI	GTMKAC	1 cut(s)	1086
Fnu4HI	GCNGC	7 cut(s)	254, 921, 1200, 1417, 1627, 1945, 1948
FokI	GGATG	6 cut(s)	1533, 1705, 1718, 1763, 1951, 2051
FriOI	GRGCYC	2 cut(s)	781, 1803
Fsp4HI	GCNGC	7 cut(s)	254, 921, 1200, 1417, 1627, 1945, 1948
FspBI	CTAG	8 cut(s)	894, 1064, 1172, 1520, 1770, 1863, 2244, 2294
GlaI	GCGC	3 cut(s)	650, 1932, 1998
GluI	GCNGC	7 cut(s)	254, 921, 1200, 1417, 1627, 1945, 1948
GsaI	CCCAGC	5 cut(s)	190, 194, 260, 281, 1659
GsuI	CTGGAG	4 cut(s)	370, 428, 1382, 1466
HaeII	RGCGCY	1 cut(s)	2000
HaeIII	GGCC	7 cut(s)	432, 441, 476, 1049, 1419, 1529, 1821
HgaI	GACGC	2 cut(s)	163, 1937
HhaI	GCGC	3 cut(s)	651, 1933, 1999
Hin1I	GRCGYC	1 cut(s)	174
Hin6I	GCGC	3 cut(s)	649, 1931, 1997
HinP1I	GCGC	3 cut(s)	649, 1931, 1997
HincII	GTYRAC	1 cut(s)	2167
HindII	GTYRAC	1 cut(s)	2167
HindIII	AAGCTT	1 cut(s)	1595
HphI	GGTGA	5 cut(s)	217, 261, 650, 1084, 1552
Hpy166II	GTNNAC	8 cut(s)	183, 284, 873, 1087, 1432, 1753, 2167, 2255
Hpy188III	TCNNGA	5 cut(s)	626, 703, 1375, 1513, 1790
Hpy8I	GTNNAC	8 cut(s)	183, 284, 873, 1087, 1432, 1753, 2167, 2255
Hpy99I	CGWCG	1 cut(s)	1040
HpyCH4III	ACNGT	3 cut(s)	520, 818, 1259
HpyCH4IV	ACGT	3 cut(s)	179, 456, 935
HpyCH4V	TGCA	5 cut(s)	183, 470, 1297, 1466, 1979
HpySE526I	ACGT	3 cut(s)	179, 456, 935
Hsp92I	GRCGYC	1 cut(s)	174
HspAI	GCGC	3 cut(s)	649, 1931, 1997
KpnI	GGTACC	1 cut(s)	207
KroI	GCCGGC	1 cut(s)	1025
KroNI	GCCGGC	1 cut(s)	1027
KspI	CCGCGG	1 cut(s)	1971
Kzo9I	GATC	8 cut(s)	112, 307, 396, 415, 589, 733, 1020, 1698
LguI	GCTCTTC	1 cut(s)	1645
LmnI	GCTCC	6 cut(s)	364, 971, 1363, 1409, 1798, 1938
Lsp1109I	GCAGC	6 cut(s)	240, 907, 1211, 1638, 1956, 1959
LweI	GCATC	5 cut(s)	1338, 1400, 1555, 1988, 2194
MabI	ACCWGGT	1 cut(s)	5
MaeI	CTAG	8 cut(s)	894, 1064, 1172, 1520, 1770, 1863, 2244, 2294
MaeII	ACGT	3 cut(s)	179, 456, 935
MaeIII	GTNAC	6 cut(s)	175, 223, 536, 1162, 1259, 1336
MalI	GATC	8 cut(s)	114, 309, 398, 417, 591, 735, 1022, 1700
MbiI	CCGCTC	2 cut(s)	118, 1002
MboI	GATC	8 cut(s)	112, 307, 396, 415, 589, 733, 1020, 1698
MflI	RGATCY	1 cut(s)	589
MhlI	GDGCHC	3 cut(s)	185, 781, 1803
MlyI	GAGTC	7 cut(s)	125, 231, 297, 610, 764, 1322, 2012
MmeI	TCCRAC	5 cut(s)	313, 339, 486, 624, 1018
Mph1103I	ATGCAT	2 cut(s)	472, 1981
MroNI	GCCGGC	1 cut(s)	1025
MseI	TTAA	3 cut(s)	570, 1784, 2156
MslI	CAYNNNNRTG	2 cut(s)	1207, 1334
MspA1I	CMGCKG	4 cut(s)	190, 253, 1416, 1970
MspR9I	CCNGG	8 cut(s)	7, 136, 166, 201, 950, 1014, 1142, 2124
Mva1269I	GAATGC	1 cut(s)	1981
MvaI	CCWGG	6 cut(s)	7, 136, 166, 201, 1014, 1142
MvnI	CGCG	5 cut(s)	704, 775, 1931, 1970, 2131
NaeI	GCCGGC	1 cut(s)	1027
NciI	CCSGG	2 cut(s)	950, 2124
NdeII	GATC	8 cut(s)	112, 307, 396, 415, 589, 733, 1020, 1698
NgoMIV	GCCGGC	1 cut(s)	1025
NheI	GCTAGC	1 cut(s)	1862
NlaIV	GGNNCC	5 cut(s)	77, 205, 1355, 1411, 1800
NmuCI	GTSAC	5 cut(s)	175, 223, 1162, 1259, 1336
NruI	TCGCGA	1 cut(s)	704
NsiI	ATGCAT	2 cut(s)	472, 1981
NspI	RCATGY	2 cut(s)	217, 1751
NspV	TTCGAA	1 cut(s)	2149
PaeR7I	CTCGAG	1 cut(s)	1373
PceI	AGGCCT	1 cut(s)	441
PciI	ACATGT	2 cut(s)	213, 1747
PciSI	GCTCTTC	1 cut(s)	1645
PcsI	WCGNNNNNNNCGW	1 cut(s)	722
PctI	GAATGC	1 cut(s)	1981
PdiI	GCCGGC	1 cut(s)	1027
PfeI	GAWTC	7 cut(s)	483, 553, 982, 988, 1075, 1300, 1552
PflFI	GACNNNGTC	1 cut(s)	1613
PflMI	CCANNNNNTGG	1 cut(s)	256
PfoI	TCCNGGA	2 cut(s)	134, 164
PkrI	GCNGC	7 cut(s)	255, 922, 1201, 1418, 1628, 1946, 1949
Ple19I	CGATCG	1 cut(s)	1023
PleI	GAGTC	7 cut(s)	125, 230, 297, 610, 764, 1321, 2011
PmaCI	CACGTG	1 cut(s)	180
PmlI	CACGTG	1 cut(s)	180
PpsI	GAGTC	7 cut(s)	125, 230, 297, 610, 764, 1321, 2011
Ppu21I	YACGTR	1 cut(s)	180
PpuMI	RGGWCCY	2 cut(s)	76, 373
PscI	ACATGT	2 cut(s)	213, 1747
PshAI	GACNNNNGTC	1 cut(s)	173
Psp1406I	AACGTT	1 cut(s)	935
Psp5II	RGGWCCY	2 cut(s)	76, 373
Psp6I	CCWGG	6 cut(s)	5, 134, 164, 199, 1012, 1140
PspCI	CACGTG	1 cut(s)	180
PspFI	CCCAGC	5 cut(s)	186, 190, 256, 277, 1655
PspGI	CCWGG	6 cut(s)	5, 134, 164, 199, 1012, 1140
PspN4I	GGNNCC	5 cut(s)	77, 205, 1355, 1411, 1800
PspPI	GGNCC	6 cut(s)	76, 373, 758, 1354, 1418, 1527
PspPPI	RGGWCCY	2 cut(s)	76, 373
PsrI	GAACNNNNNNTAC	4 cut(s)	650, 682, 925, 957
PstI	CTGCAG	1 cut(s)	1299
PstNI	CAGNNNCTG	1 cut(s)	256
PsuI	RGATCY	1 cut(s)	589
PsyI	GACNNNGTC	1 cut(s)	1613
PvuI	CGATCG	1 cut(s)	1023
PvuII	CAGCTG	2 cut(s)	190, 253
RruI	TCGCGA	1 cut(s)	704
RsaI	GTAC	8 cut(s)	205, 898, 1062, 1433, 1883, 1921, 2128, 2305
RsaNI	GTAC	8 cut(s)	204, 897, 1061, 1432, 1882, 1920, 2127, 2304
RseI	CAYNNNNRTG	2 cut(s)	1207, 1334
Rsr2I	CGGWCCG	1 cut(s)	758
RsrII	CGGWCCG	1 cut(s)	758
SacII	CCGCGG	1 cut(s)	1971
SapI	GCTCTTC	1 cut(s)	1645
SaqAI	TTAA	3 cut(s)	570, 1784, 2156
SatI	GCNGC	7 cut(s)	254, 921, 1200, 1417, 1627, 1945, 1948
Sau3AI	GATC	8 cut(s)	112, 307, 396, 415, 589, 733, 1020, 1698
Sau96I	GGNCC	6 cut(s)	76, 373, 758, 1354, 1418, 1527
ScaI	AGTACT	1 cut(s)	2305
SchI	GAGTC	7 cut(s)	125, 231, 297, 610, 764, 1322, 2012
ScrFI	CCNGG	8 cut(s)	7, 136, 166, 201, 950, 1014, 1142, 2124
SduI	GDGCHC	3 cut(s)	185, 781, 1803
SexAI	ACCWGGT	1 cut(s)	5
SfaNI	GCATC	5 cut(s)	1338, 1400, 1555, 1988, 2194
SfcI	CTRYAG	1 cut(s)	1295
SfiI	GGCCNNNNNGGCC	1 cut(s)	438
Sfr274I	CTCGAG	1 cut(s)	1373
Sfr303I	CCGCGG	1 cut(s)	1971
SfuI	TTCGAA	1 cut(s)	2149
SgrBI	CCGCGG	1 cut(s)	1971
SinI	GGWCC	4 cut(s)	76, 373, 758, 1354
SlaI	CTCGAG	1 cut(s)	1373
SmiMI	CAYNNNNRTG	2 cut(s)	1207, 1334
SmlI	CTYRAG	3 cut(s)	1112, 1373, 2066
SmoI	CTYRAG	3 cut(s)	1112, 1373, 2066
SpeI	ACTAGT	1 cut(s)	893
SseBI	AGGCCT	1 cut(s)	441
SspMI	CTAG	8 cut(s)	894, 1064, 1172, 1520, 1770, 1863, 2244, 2294
StuI	AGGCCT	1 cut(s)	441
StyD4I	CCNGG	8 cut(s)	5, 134, 164, 199, 948, 1012, 1140, 2122
TaaI	ACNGT	3 cut(s)	520, 818, 1259
TaiI	ACGT	3 cut(s)	182, 459, 938
TaqI	TCGA	7 cut(s)	129, 306, 627, 725, 1374, 1512, 2149
TaqII	GACCGA	1 cut(s)	775
TatI	WGTACW	3 cut(s)	1060, 1431, 2303
TauI	GCSGC	1 cut(s)	1419
TfiI	GAWTC	7 cut(s)	483, 553, 982, 988, 1075, 1300, 1552
Tru1I	TTAA	3 cut(s)	570, 1784, 2156
Tru9I	TTAA	3 cut(s)	570, 1784, 2156
TscAI	CASTG	7 cut(s)	523, 568, 823, 1264, 1386, 1446, 1473
TseFI	GTSAC	5 cut(s)	175, 223, 1162, 1259, 1336
TseI	GCWGC	6 cut(s)	253, 920, 1199, 1626, 1944, 1947
Tsp45I	GTSAC	5 cut(s)	175, 223, 1162, 1259, 1336
TspDTI	ATGAA	4 cut(s)	1197, 1293, 1709, 2288
TspGWI	ACGGA	2 cut(s)	1770, 2150
TspRI	CASTG	7 cut(s)	523, 568, 823, 1264, 1386, 1446, 1473
Tth111I	GACNNNGTC	1 cut(s)	1613
Van91I	CCANNNNNTGG	1 cut(s)	256
VneI	GTGCAC	1 cut(s)	181
VpaK11BI	GGWCC	4 cut(s)	76, 373, 758, 1354
XagI	CCTNNNNNAGG	1 cut(s)	10
XapI	RAATTY	4 cut(s)	492, 686, 2145, 2151
XceI	RCATGY	2 cut(s)	217, 1751
XcmI	CCANNNNNNNNNTGG	2 cut(s)	583, 2031
XhoI	CTCGAG	1 cut(s)	1373
XmiI	GTMKAC	1 cut(s)	1086
XspI	CTAG	8 cut(s)	894, 1064, 1172, 1520, 1770, 1863, 2244, 2294
ZrmI	AGTACT	1 cut(s)	2305
Zsp2I	ATGCAT	2 cut(s)	472, 1981

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.