Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0011220

Physical Location & Seq

Genomic Coordinates Reverse (-)

10779176 .. 10782461

Gene Structure 3286 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g08270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGATCTGGTGGAGGGTTGCTTCCATTTTTCTTCCTCTTCCTCTTCTTCTTCTTCTTCTTCTTCCCTCAGCATTTGCTCTCAACTCAGATGGGATTCTGCTGCTTTCCTTCAAGTACTCAATCATCAGCGACCCGCTCTCAGTCCTCGACAACTGGAACTACGAAGACGAAACCCCATGTTCCTGGATTGGCGTCACGTGCACCCAGCTGGGAAACCCAGGTACCCCAGACATGTTCAGAGTCACCAGCTTAGTCCTCCCCAACAGCCACCTGGTGGGTTCAATCTCACCCGAGCTGGGTTCACTCCAACACATCCAGAGACTCGATCTTTCCAAGAATTTCTTCAATGGGTCTCTGCCTGTCACCCTCTTCAATGCCTCAGAGCTTAAGGTCCTCTCCCTCTCCAACAATTTCATCTCTGGCGACCTGCCGGATGAGCTCGTCTCGGGCCTCAAAAGCCTCCAGTTTCTCAACGTCTCCGACAATGACTTGGCCGGAGACATCCCAGAAAATCTCACCTCTTTACAAAACCTCACCGTAATTTCTCTGAGGAGTAATTACTTTACCGGAAATCTCCCAGCTGGGTTTAATTCCGTTGAGGTTTTAGATCTCTCCTCCAACCTCCTCAACGGGACTCTGCCTCTCGACTTCGGCGGCGAAAACCTGCGCTACTTGAACCTCTCCTACAACAAAATCTCTGGAAAAATTCCTGTTGGTTTCGTGAAAAGGGTTTCGGAGAACTCCAACATTGATCTCTCCTTCAACAATTTGACCGGACCGATTCCAGACTCGCCGGCTCTGCTCAGTCAGAAAACAGAGCAGTTTGCAGGAAACAGTGAGCTTTGTGGGAAGCCATTGAAGACCCTCTGTCCCATTCCTTCTACTCTGTCCACACCGCCGAATATGGCAACTAGTACCAGTTCTTCTTCTCCGGCGATTGCTGCCATTCCCAGAACATTTGATAATGGCCCAGAAACGAACACTTCTGGGGGAGCAAATGGGACTCGGAATCAATCGCAGACCGGGCTGAAACCCGGCACGATCGCCGGCATTGTGGTCGGGGACTTGGCCGGAATAGCTGTACTTGGATTGGTCATTCTCTATGTCTACCACGTCAGGAAGAGAAAAAGTCTCAACTCAAGAGTTGTCACTAAACAAGACCCAGATCCAAAAGCAAAATCCGCAGCATGGTCATGCATAACAATAAAAGGGGAAGAGAAATCAGAGGCAACAAGCTCAGACAGTGACCATGATGACAAAAATGGACCAGTAGGTGGTCTGCACAACCCGAACGGGGAGGCTCAGAAAAGCAGCGTCTTGGTGACGGTAGAAGGTGGGGACCAACCGGAGCTTGAGCTCGAGACACTGCTGAAAGCATCAGCTTATATATTGGGAGCCAGTGGCCCAAGCATTGTGTACAAGGCAGTGCTGGAGGACAAGACTGAGCTTGCTGTGAGGAGGATAGGAGAGTGTAGGGTTTTGAAGATGAGGGATTTCGAGAGCCAAGTGCGGGCCATTGCAAAGGTGAGGCATCCGAATCTGGTTAGGGTTCGTGGGTTTTACTGGGGAGACGATGAGAAGCTTGTTATCTATGACTATGTCTCCAATGGCAGCCTTGCCACCACCAACACCAGAAGAGCAGGGTCGTCGCCGTGTCATCTACCTCTCGAAACTCGGATGAAGATAGCAAGAGGGGTTGCGAGGGGGCTGGCGTACATCCATGAGAAGAAACATGTGCATGGAAATATCAAACCTAGCAACATTCTTTTAAATTCTGATATGGAACCTATAATTAGCGATTTCGGCCTTGATAAGCTGGTGTTAGGTAACACGATTGGTCAAAAAGCTAGTGGTTCAGCCCGGGGGTACTTTGGAAGCCTAAAATCCACGGCCACTCGCGAAGGTACGCATGATGTGCTCCCAATTGGTGGCAGCCCCGCCGCAATGCCATCCGCGGGAGCGGGGAGCGCATCTTCGCCGTATCAAGCACCGGAGTCCCTAAAGAACTTGAAGCCAAACCCCAAGTGGGATGTGTATTCTTTTGGTATAGTCTTGCTTGAGCTTCTCACGGGAAGGATACCTTCGGAGCGAGAATTGGTTGCCCAATGGACACCGGGTTCGCTGACGGAGGAGAAAATTCGAATTTTAATAATGGTTGACGTGGTGATTAGGGCTGAGGTGGAAGGCAGGGAAGATGCCTTGCTGGCTTGTTTGAAATTAGGGTTTAGTTGTGCTAGTTTTAGCCCACAAAAAAGACCAACCATGAAAGAAGTCTTCCAAATCCTAGACAAAGTAGTTCCACTTCTTCAAACTGATGTATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0007154 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010029 GO:0010030 GO:0010035 GO:0010646 GO:0010647 GO:0016020 GO:0023051 GO:0023056 GO:0031668 GO:0033554 GO:0042221 GO:0042631 GO:0044464 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071944 GO:0104004 GO:1900140 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1905957 GO:1905959 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

775

Amino Acids

83.44

Weight (kDa)

6.32

Isoelectric Point (pI)

34.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	27 - 68	2.4e-12	Leucine rich repeat N-terminal domain
LRR_14	PF23598	93 - 184	6.8e-06	Leucine-rich repeat region
LRR_8	PF13855	104 - 164	9.6e-07	Leucine rich repeat
LRR_4	PF12799	128 - 168	7.2e-06	Leucine Rich repeats (2 copies)
Pkinase	PF00069	464 - 607	4.5e-18	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	471 - 607	5.3e-19	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	657 - 760	3.8e-08	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	657 - 761	4.9e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011220)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15300 AT4G34220
fragaria_vesca	FvH4_2g05270
malus_domestica	MD05G1088500.v1.1 MD10G1096400.v1.1
prunus_persica	Prupe.8G134500_v2.0.a1
pyrus_communis	pycom05g08790 pycom10g08270
rosa_chinensis	RchiOBHm_Chr6g0255401
rosa_laevigata	RLG00000014841
rosa_multiflora	Rmu_co8383769.1_g000001 Rmu_sc0002360.1_g000015
rosa_roxburghii	Rroxscaffold_7G00210020
rosa_rugosa	Rorug05G0558100.1 Rorug05G0558200
rosa_samantha	Rh6AG075300 Rh6BG068400 Rh6CG068000 Rh6DG064700
rosa_wichuraiana	Rw6G006620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	435, 672
Acc65I	GGTACC	1 cut(s)	221
AccB1I	GGYRCC	1 cut(s)	221
AccB7I	CCANNNNNTGG	3 cut(s)	274, 1274, 1928
AccBSI	CCGCTC	2 cut(s)	136, 1961
AccI	GTMKAC	1 cut(s)	1107
AccII	CGCG	2 cut(s)	1899, 1955
AclWI	GGATC	1 cut(s)	1160
AcoI	YGGCCR	3 cut(s)	492, 1068, 1890
AcsI	RAATTY	5 cut(s)	337, 705, 1771, 2136, 2142
AcvI	CACGTG	1 cut(s)	198
AcyI	GRCGYC	1 cut(s)	192
AdeI	CACNNNGTG	1 cut(s)	274
AfaI	GTAC	8 cut(s)	116, 223, 916, 1083, 1418, 1715, 1868, 1906
AfiI	CCNNNNNNNGG	9 cut(s)	274, 296, 1188, 1274, 1294, 1510, 1928, 1955, 2026
AflII	CTTAAG	1 cut(s)	386
AflIII	ACRYGT	2 cut(s)	231, 1732
AhdI	GACNNNNNGTC	1 cut(s)	867
AhlI	ACTAGT	1 cut(s)	911
AjiI	CACGTC	2 cut(s)	1114, 2161
AjnI	CCWGG	3 cut(s)	182, 217, 270
AjuI	GAANNNNNNNTTGG	2 cut(s)	326, 358
AloI	GAACNNNNNNTCC	2 cut(s)	2101, 2133
Alw21I	GWGCWC	4 cut(s)	203, 441, 1359, 1920
Alw26I	GTCTC	9 cut(s)	313, 357, 448, 481, 492, 1136, 1355, 1563, 1606
Alw44I	GTGCAC	1 cut(s)	199
AlwI	GGATC	1 cut(s)	1160
Ama87I	CYCGRG	4 cut(s)	290, 445, 1358, 1860
AoxI	GGCC	8 cut(s)	448, 492, 967, 1068, 1402, 1512, 1804, 1890
ApaLI	GTGCAC	1 cut(s)	199
ApeKI	GCWGC	6 cut(s)	100, 941, 1184, 1311, 1611, 1932
ApoI	RAATTY	5 cut(s)	337, 705, 1771, 2136, 2142
ArsI	GACNNNNNNTTYG	2 cut(s)	2101, 2133
Asp700I	GAANNNNTTC	1 cut(s)	341
Asp718I	GGTACC	1 cut(s)	221
AspLEI	GCGC	2 cut(s)	669, 1970
AspS9I	GGNCC	8 cut(s)	391, 448, 776, 968, 1265, 1339, 1403, 1512
AsuC2I	CCSGG	5 cut(s)	1024, 1035, 1861, 1862, 2115
AsuHPI	GGTGA	8 cut(s)	235, 279, 355, 508, 526, 1333, 1537, 2176
AsuII	TTCGAA	1 cut(s)	2140
AvaI	CYCGRG	4 cut(s)	290, 445, 1358, 1860
AvaII	GGWCC	4 cut(s)	391, 776, 1265, 1339
BaeGI	GKGCMC	1 cut(s)	203
BaeI	ACNNNNGTAYC	2 cut(s)	205, 238
BanI	GGYRCC	1 cut(s)	221
BanII	GRGCYC	2 cut(s)	441, 1359
BarI	GAAGNNNNNNTAC	4 cut(s)	907, 939, 2286, 2318
BbrPI	CACGTG	1 cut(s)	198
BbsI	GAAGAC	3 cut(s)	171, 866, 2266
Bbv12I	GWGCWC	4 cut(s)	203, 441, 1359, 1920
BbvCI	CCTCAGC	2 cut(s)	67, 2175
BbvI	GCAGC	6 cut(s)	87, 928, 1196, 1323, 1623, 1944
BccI	CCATC	2 cut(s)	83, 1957
BceAI	ACGGC	3 cut(s)	1636, 1905, 1963
BcgI	CGANNNNNNTGC	4 cut(s)	1039, 1073, 1629, 1663
BciT130I	CCWGG	3 cut(s)	184, 219, 272
BciVI	GTATCC	1 cut(s)	2070
BcnI	CCSGG	5 cut(s)	1024, 1035, 1861, 1862, 2115
BcoDI	GTCTC	9 cut(s)	313, 357, 448, 481, 492, 1136, 1355, 1563, 1606
BcuI	ACTAGT	1 cut(s)	911
BfaI	CTAG	5 cut(s)	912, 1755, 1848, 2235, 2285
BfrI	CTTAAG	1 cut(s)	386
BfuAI	ACCTGC	2 cut(s)	435, 672
BfuI	GTATCC	1 cut(s)	2070
BglI	GCCNNNNNGGC	1 cut(s)	2204
BglII	AGATCT	1 cut(s)	607
BisI	GCNGC	8 cut(s)	101, 655, 942, 1185, 1312, 1612, 1933, 1941
BlsI	GCNGC	8 cut(s)	102, 656, 943, 1186, 1313, 1613, 1934, 1942
BmcAI	AGTACT	1 cut(s)	116
Bme1390I	CCNGG	8 cut(s)	184, 219, 272, 1024, 1035, 1861, 1862, 2115
Bme18I	GGWCC	4 cut(s)	391, 776, 1265, 1339
BmeRI	GACNNNNNGTC	1 cut(s)	867
BmeT110I	CYCGRG	4 cut(s)	290, 445, 1358, 1860
BmgBI	CACGTC	2 cut(s)	1114, 2161
BmgT120I	GGNCC	8 cut(s)	391, 448, 776, 968, 1265, 1339, 1403, 1512
BmiI	GGNNCC	4 cut(s)	223, 1340, 1396, 1785
BmrFI	CCNGG	8 cut(s)	184, 219, 272, 1024, 1035, 1861, 1862, 2115
BmrI	ACTGGG	1 cut(s)	1573
BmsI	GCATC	4 cut(s)	1385, 1540, 1979, 2185
BmuI	ACTGGG	1 cut(s)	1573
BpiI	GAAGAC	3 cut(s)	171, 866, 2266
BplI	GAGNNNNNCTC	2 cut(s)	428, 460
BpmI	CTGGAG	2 cut(s)	446, 1451
Bpu10I	CCTNAGC	2 cut(s)	67, 2175
Bpu14I	TTCGAA	1 cut(s)	2140
BpuEI	CTTGAG	3 cut(s)	1123, 1373, 2078
BpuMI	CCSGG	5 cut(s)	1024, 1035, 1861, 1862, 2115
BsaAI	YACGTR	1 cut(s)	198
BsaHI	GRCGYC	1 cut(s)	192
BsaI	GGTCTC	1 cut(s)	357
BsaJI	CCNNGG	5 cut(s)	217, 1860, 1861, 1887, 1953
BsaWI	WCCGGW	4 cut(s)	566, 773, 1345, 1990
BsaXI	ACNNNNNCTCC	2 cut(s)	1586, 1616
Bsc4I	CCNNNNNNNGG	9 cut(s)	274, 296, 1188, 1274, 1294, 1510, 1928, 1955, 2026
Bse118I	RCCGGY	2 cut(s)	793, 1046
Bse1I	ACTGG	6 cut(s)	158, 463, 918, 1268, 1398, 1568
Bse3DI	GCAATG	2 cut(s)	1515, 1950
BseBI	CCWGG	3 cut(s)	184, 219, 272
BseDI	CCNNGG	5 cut(s)	217, 1860, 1861, 1887, 1953
BseGI	GGATG	8 cut(s)	312, 439, 501, 1531, 1683, 1716, 1949, 2035
BseLI	CCNNNNNNNGG	9 cut(s)	274, 296, 1188, 1274, 1294, 1510, 1928, 1955, 2026
BseMI	GCAATG	2 cut(s)	1515, 1950
BseNI	ACTGG	6 cut(s)	158, 463, 918, 1268, 1398, 1568
BseRI	GAGGAG	5 cut(s)	565, 604, 614, 1471, 2144
BseSI	GKGCMC	1 cut(s)	203
BseXI	GCAGC	6 cut(s)	87, 928, 1196, 1323, 1623, 1944
BseYI	CCCAGC	5 cut(s)	204, 208, 295, 577, 581
BsgI	GTGCAG	1 cut(s)	1265
Bsh1236I	CGCG	2 cut(s)	1899, 1955
Bsh1285I	CGRYCG	1 cut(s)	1044
BshFI	GGCC	8 cut(s)	450, 494, 969, 1070, 1404, 1514, 1806, 1892
BshNI	GGYRCC	1 cut(s)	221
BsiEI	CGRYCG	1 cut(s)	1044
BsiHKAI	GWGCWC	4 cut(s)	203, 441, 1359, 1920
BsiHKCI	CYCGRG	4 cut(s)	290, 445, 1358, 1860
BslFI	GGGAC	6 cut(s)	646, 855, 1015, 1076, 1352, 1981
BslI	CCNNNNNNNGG	9 cut(s)	274, 296, 1188, 1274, 1294, 1510, 1928, 1955, 2026
BsmAI	GTCTC	9 cut(s)	313, 357, 448, 481, 492, 1136, 1355, 1563, 1606
BsmBI	CGTCTC	3 cut(s)	448, 481, 1563
BsmFI	GGGAC	6 cut(s)	646, 855, 1015, 1076, 1352, 1981
BsnI	GGCC	8 cut(s)	450, 494, 969, 1070, 1404, 1514, 1806, 1892
Bso31I	GGTCTC	1 cut(s)	357
BsoBI	CYCGRG	4 cut(s)	290, 445, 1358, 1860
Bsp119I	TTCGAA	1 cut(s)	2140
Bsp1286I	GDGCHC	4 cut(s)	203, 441, 1359, 1920
Bsp1407I	TGTACA	1 cut(s)	1416
Bsp143I	GATC	6 cut(s)	3, 325, 607, 751, 1041, 1165
Bsp68I	TCGCGA	1 cut(s)	1899
BspANI	GGCC	8 cut(s)	450, 494, 969, 1070, 1404, 1514, 1806, 1892
BspFNI	CGCG	2 cut(s)	1899, 1955
BspLI	GGNNCC	4 cut(s)	223, 1340, 1396, 1785
BspMI	ACCTGC	2 cut(s)	435, 672
BspPI	GGATC	1 cut(s)	1160
BspQI	GCTCTTC	1 cut(s)	1630
BspT104I	TTCGAA	1 cut(s)	2140
BspT107I	GGYRCC	1 cut(s)	221
BspTI	CTTAAG	1 cut(s)	386
BspTNI	GGTCTC	1 cut(s)	357
BsrBI	CCGCTC	2 cut(s)	136, 1961
BsrDI	GCAATG	2 cut(s)	1515, 1950
BsrFI	RCCGGY	2 cut(s)	793, 1046
BsrGI	TGTACA	1 cut(s)	1416
BsrI	ACTGG	6 cut(s)	158, 463, 918, 1268, 1398, 1568
BssAI	RCCGGY	2 cut(s)	793, 1046
BssECI	CCNNGG	5 cut(s)	217, 1860, 1861, 1887, 1953
BssMI	GATC	6 cut(s)	3, 325, 607, 751, 1041, 1165
BssNI	GRCGYC	1 cut(s)	192
Bst2UI	CCWGG	3 cut(s)	184, 219, 272
Bst4CI	ACNGT	4 cut(s)	538, 836, 1244, 1327
Bst6I	CTCTTC	6 cut(s)	42, 48, 374, 1115, 1209, 1630
BstACI	GRCGYC	1 cut(s)	192
BstAFI	CTTAAG	1 cut(s)	386
BstAPI	GCANNNNNTGC	1 cut(s)	1915
BstAUI	TGTACA	1 cut(s)	1416
BstBAI	YACGTR	1 cut(s)	198
BstBI	TTCGAA	1 cut(s)	2140
BstC8I	GCNNGC	6 cut(s)	795, 1048, 1449, 1512, 1710, 2205
BstDSI	CCRYGG	2 cut(s)	1887, 1953
BstF5I	GGATG	8 cut(s)	312, 439, 501, 1531, 1683, 1716, 1949, 2035
BstFNI	CGCG	2 cut(s)	1899, 1955
BstHHI	GCGC	2 cut(s)	669, 1970
BstKTI	GATC	6 cut(s)	6, 328, 610, 754, 1044, 1168
BstMAI	GTCTC	9 cut(s)	313, 357, 448, 481, 492, 1136, 1355, 1563, 1606
BstMBI	GATC	6 cut(s)	3, 325, 607, 751, 1041, 1165
BstMCI	CGRYCG	1 cut(s)	1044
BstNI	CCWGG	3 cut(s)	184, 219, 272
BstNSI	RCATGY	2 cut(s)	235, 1736
BstSCI	CCNGG	8 cut(s)	182, 217, 270, 1022, 1033, 1859, 1860, 2113
BstSLI	GKGCMC	1 cut(s)	203
BstUI	CGCG	2 cut(s)	1899, 1955
BstV1I	GCAGC	6 cut(s)	87, 928, 1196, 1323, 1623, 1944
BstV2I	GAAGAC	3 cut(s)	171, 866, 2266
BstX2I	RGATCY	2 cut(s)	607, 1165
BstXI	CCANNNNNNTGG	1 cut(s)	183
BstYI	RGATCY	2 cut(s)	607, 1165
BsuI	GTATCC	1 cut(s)	2070
BsuRI	GGCC	8 cut(s)	450, 494, 969, 1070, 1404, 1514, 1806, 1892
BtgI	CCRYGG	2 cut(s)	1887, 1953
BtrI	CACGTC	2 cut(s)	1114, 2161
BtsCI	GGATG	8 cut(s)	312, 439, 501, 1531, 1683, 1716, 1949, 2035
BtsI	GCAGTG	2 cut(s)	1364, 1431
BtsIMutI	CAGTG	5 cut(s)	841, 1249, 1364, 1405, 1431
BtuMI	TCGCGA	1 cut(s)	1899
BveI	ACCTGC	2 cut(s)	435, 672
Cac8I	GCNNGC	6 cut(s)	795, 1048, 1449, 1512, 1710, 2205
CfoI	GCGC	2 cut(s)	669, 1970
Cfr10I	RCCGGY	2 cut(s)	793, 1046
Cfr13I	GGNCC	8 cut(s)	391, 448, 776, 968, 1265, 1339, 1403, 1512
Cfr42I	CCGCGG	1 cut(s)	1956
Cfr9I	CCCGGG	1 cut(s)	1860
CpoI	CGGWCCG	1 cut(s)	776
CseI	GACGC	2 cut(s)	181, 1303
CsiI	ACCWGGT	1 cut(s)	270
Csp6I	GTAC	8 cut(s)	115, 222, 915, 1082, 1417, 1714, 1867, 1905
CspI	CGGWCCG	1 cut(s)	776
CviQI	GTAC	8 cut(s)	115, 222, 915, 1082, 1417, 1714, 1867, 1905
DpnI	GATC	6 cut(s)	5, 327, 609, 753, 1043, 1167
DpnII	GATC	6 cut(s)	3, 325, 607, 751, 1041, 1165
DraI	TTTAAA	1 cut(s)	1770
DraIII	CACNNNGTG	1 cut(s)	274
DriI	GACNNNNNGTC	1 cut(s)	867
EaeI	YGGCCR	3 cut(s)	492, 1068, 1890
Eam1104I	CTCTTC	6 cut(s)	42, 48, 374, 1115, 1209, 1630
Eam1105I	GACNNNNNGTC	1 cut(s)	867
EarI	CTCTTC	6 cut(s)	42, 48, 374, 1115, 1209, 1630
Ecl136II	GAGCTC	2 cut(s)	439, 1357
Eco24I	GRGCYC	2 cut(s)	441, 1359
Eco31I	GGTCTC	1 cut(s)	357
Eco47I	GGWCC	4 cut(s)	391, 776, 1265, 1339
Eco53kI	GAGCTC	2 cut(s)	439, 1357
Eco72I	CACGTG	1 cut(s)	198
Eco88I	CYCGRG	4 cut(s)	290, 445, 1358, 1860
EcoICRI	GAGCTC	2 cut(s)	439, 1357
EcoO109I	RGGNCCY	1 cut(s)	391
EcoRII	CCWGG	3 cut(s)	182, 217, 270
EcoT22I	ATGCAT	1 cut(s)	1199
EcoT38I	GRGCYC	2 cut(s)	441, 1359
Esp3I	CGTCTC	3 cut(s)	448, 481, 1563
FalI	AAGNNNNNCTT	4 cut(s)	326, 358, 2065, 2097
FaqI	GGGAC	6 cut(s)	646, 855, 1015, 1076, 1352, 1981
FauI	CCCGC	5 cut(s)	141, 1503, 1945, 1948, 1954
FblI	GTMKAC	1 cut(s)	1107
Fnu4HI	GCNGC	8 cut(s)	101, 655, 942, 1185, 1312, 1612, 1933, 1941
FokI	GGATG	8 cut(s)	299, 446, 488, 1518, 1690, 1703, 1936, 2042
FriOI	GRGCYC	2 cut(s)	441, 1359
Fsp4HI	GCNGC	8 cut(s)	101, 655, 942, 1185, 1312, 1612, 1933, 1941
FspBI	CTAG	5 cut(s)	912, 1755, 1848, 2235, 2285
GlaI	GCGC	2 cut(s)	668, 1969
GluI	GCNGC	8 cut(s)	101, 655, 942, 1185, 1312, 1612, 1933, 1941
GsaI	CCCAGC	5 cut(s)	208, 212, 299, 581, 585
GsuI	CTGGAG	2 cut(s)	446, 1451
HaeIII	GGCC	8 cut(s)	450, 494, 969, 1070, 1404, 1514, 1806, 1892
HgaI	GACGC	2 cut(s)	181, 1303
HhaI	GCGC	2 cut(s)	669, 1970
Hin1I	GRCGYC	1 cut(s)	192
Hin6I	GCGC	2 cut(s)	667, 1968
HinP1I	GCGC	2 cut(s)	667, 1968
HincII	GTYRAC	1 cut(s)	2158
HindII	GTYRAC	1 cut(s)	2158
HindIII	AAGCTT	1 cut(s)	1580
HphI	GGTGA	8 cut(s)	235, 279, 355, 508, 526, 1333, 1537, 2176
Hpy166II	GTNNAC	6 cut(s)	201, 302, 891, 1108, 1417, 2158
Hpy8I	GTNNAC	6 cut(s)	201, 302, 891, 1108, 1417, 2158
Hpy99I	CGWCG	1 cut(s)	1651
HpyAV	CCTTC	8 cut(s)	118, 769, 888, 1325, 1895, 2067, 2091, 2177
HpyCH4III	ACNGT	4 cut(s)	538, 836, 1244, 1327
HpyCH4IV	ACGT	4 cut(s)	197, 474, 1113, 2160
HpyCH4V	TGCA	6 cut(s)	201, 827, 1197, 1282, 1520, 1738
HpySE526I	ACGT	4 cut(s)	197, 474, 1113, 2160
Hsp92I	GRCGYC	1 cut(s)	192
HspAI	GCGC	2 cut(s)	667, 1968
KpnI	GGTACC	1 cut(s)	225
KroI	GCCGGC	2 cut(s)	793, 1046
KroNI	GCCGGC	2 cut(s)	795, 1048
KspI	CCGCGG	1 cut(s)	1956
Kzo9I	GATC	6 cut(s)	3, 325, 607, 751, 1041, 1165
LguI	GCTCTTC	1 cut(s)	1630
LmnI	GCTCC	7 cut(s)	992, 1348, 1394, 1923, 1958, 1965, 2086
Lsp1109I	GCAGC	6 cut(s)	87, 928, 1196, 1323, 1623, 1944
LweI	GCATC	4 cut(s)	1385, 1540, 1979, 2185
MabI	ACCWGGT	1 cut(s)	270
MaeI	CTAG	5 cut(s)	912, 1755, 1848, 2235, 2285
MaeII	ACGT	4 cut(s)	197, 474, 1113, 2160
MaeIII	GTNAC	7 cut(s)	193, 241, 361, 1147, 1244, 1321, 1826
MalI	GATC	6 cut(s)	5, 327, 609, 753, 1043, 1167
MbiI	CCGCTC	2 cut(s)	136, 1961
MboI	GATC	6 cut(s)	3, 325, 607, 751, 1041, 1165
MfeI	CAATTG	1 cut(s)	1923
MflI	RGATCY	2 cut(s)	607, 1165
MhlI	GDGCHC	4 cut(s)	203, 441, 1359, 1920
MlyI	GAGTC	6 cut(s)	249, 315, 628, 782, 997, 2003
MmeI	TCCRAC	5 cut(s)	331, 429, 504, 642, 768
Mph1103I	ATGCAT	1 cut(s)	1199
MroNI	GCCGGC	2 cut(s)	793, 1046
MroXI	GAANNNNTTC	1 cut(s)	341
MseI	TTAA	4 cut(s)	387, 588, 1769, 2147
MslI	CAYNNNNRTG	2 cut(s)	1192, 1737
MspA1I	CMGCKG	3 cut(s)	208, 581, 1955
MspCI	CTTAAG	1 cut(s)	386
MspR9I	CCNGG	8 cut(s)	184, 219, 272, 1024, 1035, 1861, 1862, 2115
MunI	CAATTG	1 cut(s)	1923
MvaI	CCWGG	3 cut(s)	184, 219, 272
MvnI	CGCG	2 cut(s)	1899, 1955
NaeI	GCCGGC	2 cut(s)	795, 1048
NciI	CCSGG	5 cut(s)	1024, 1035, 1861, 1862, 2115
NdeII	GATC	6 cut(s)	3, 325, 607, 751, 1041, 1165
NgoMIV	GCCGGC	2 cut(s)	793, 1046
NlaIV	GGNNCC	4 cut(s)	223, 1340, 1396, 1785
NmuCI	GTSAC	6 cut(s)	193, 241, 361, 1147, 1244, 1321
NruI	TCGCGA	1 cut(s)	1899
NsiI	ATGCAT	1 cut(s)	1199
NspI	RCATGY	2 cut(s)	235, 1736
NspV	TTCGAA	1 cut(s)	2140
PaeR7I	CTCGAG	1 cut(s)	1358
PciI	ACATGT	2 cut(s)	231, 1732
PciSI	GCTCTTC	1 cut(s)	1630
PdiI	GCCGGC	2 cut(s)	795, 1048
PdmI	GAANNNNTTC	1 cut(s)	341
PfeI	GAWTC	4 cut(s)	94, 781, 1009, 1537
PflFI	GACNNNGTC	1 cut(s)	1598
PflMI	CCANNNNNTGG	3 cut(s)	274, 1274, 1928
PfoI	TCCNGGA	1 cut(s)	182
PkrI	GCNGC	8 cut(s)	102, 656, 943, 1186, 1313, 1613, 1934, 1942
Ple19I	CGATCG	1 cut(s)	1044
PleI	GAGTC	6 cut(s)	248, 315, 628, 782, 997, 2002
PmaCI	CACGTG	1 cut(s)	198
PmlI	CACGTG	1 cut(s)	198
PpsI	GAGTC	6 cut(s)	248, 315, 628, 782, 997, 2002
Ppu21I	YACGTR	1 cut(s)	198
PpuMI	RGGWCCY	1 cut(s)	391
PscI	ACATGT	2 cut(s)	231, 1732
Psp124BI	GAGCTC	2 cut(s)	441, 1359
Psp5II	RGGWCCY	1 cut(s)	391
Psp6I	CCWGG	3 cut(s)	182, 217, 270
PspCI	CACGTG	1 cut(s)	198
PspFI	CCCAGC	5 cut(s)	204, 208, 295, 577, 581
PspGI	CCWGG	3 cut(s)	182, 217, 270
PspN4I	GGNNCC	4 cut(s)	223, 1340, 1396, 1785
PspPI	GGNCC	8 cut(s)	391, 448, 776, 968, 1265, 1339, 1403, 1512
PspPPI	RGGWCCY	1 cut(s)	391
PsrI	GAACNNNNNNTAC	2 cut(s)	668, 700
PsuI	RGATCY	2 cut(s)	607, 1165
PsyI	GACNNNGTC	1 cut(s)	1598
PvuI	CGATCG	1 cut(s)	1044
PvuII	CAGCTG	2 cut(s)	208, 581
RruI	TCGCGA	1 cut(s)	1899
RsaI	GTAC	8 cut(s)	116, 223, 916, 1083, 1418, 1715, 1868, 1906
RsaNI	GTAC	8 cut(s)	115, 222, 915, 1082, 1417, 1714, 1867, 1905
RseI	CAYNNNNRTG	2 cut(s)	1192, 1737
Rsr2I	CGGWCCG	1 cut(s)	776
RsrII	CGGWCCG	1 cut(s)	776
SacI	GAGCTC	2 cut(s)	441, 1359
SacII	CCGCGG	1 cut(s)	1956
SapI	GCTCTTC	1 cut(s)	1630
SaqAI	TTAA	4 cut(s)	387, 588, 1769, 2147
SatI	GCNGC	8 cut(s)	101, 655, 942, 1185, 1312, 1612, 1933, 1941
Sau3AI	GATC	6 cut(s)	3, 325, 607, 751, 1041, 1165
Sau96I	GGNCC	8 cut(s)	391, 448, 776, 968, 1265, 1339, 1403, 1512
ScaI	AGTACT	1 cut(s)	116
SchI	GAGTC	6 cut(s)	249, 315, 628, 782, 997, 2003
ScrFI	CCNGG	8 cut(s)	184, 219, 272, 1024, 1035, 1861, 1862, 2115
SduI	GDGCHC	4 cut(s)	203, 441, 1359, 1920
SexAI	ACCWGGT	1 cut(s)	270
SfaNI	GCATC	4 cut(s)	1385, 1540, 1979, 2185
Sfr274I	CTCGAG	1 cut(s)	1358
Sfr303I	CCGCGG	1 cut(s)	1956
SfuI	TTCGAA	1 cut(s)	2140
SgrBI	CCGCGG	1 cut(s)	1956
SinI	GGWCC	4 cut(s)	391, 776, 1265, 1339
SlaI	CTCGAG	1 cut(s)	1358
SmaI	CCCGGG	1 cut(s)	1862
SmiMI	CAYNNNNRTG	2 cut(s)	1192, 1737
SmlI	CTYRAG	5 cut(s)	386, 1138, 1352, 1358, 2057
SmoI	CTYRAG	5 cut(s)	386, 1138, 1352, 1358, 2057
SpeI	ACTAGT	1 cut(s)	911
SspMI	CTAG	5 cut(s)	912, 1755, 1848, 2235, 2285
SstI	GAGCTC	2 cut(s)	441, 1359
StyD4I	CCNGG	8 cut(s)	182, 217, 270, 1022, 1033, 1859, 1860, 2113
TaaI	ACNGT	4 cut(s)	538, 836, 1244, 1327
TaiI	ACGT	4 cut(s)	200, 477, 1116, 2163
TaqI	TCGA	7 cut(s)	147, 324, 645, 1359, 1497, 1668, 2140
TaqII	GACCGA	1 cut(s)	793
TatI	WGTACW	3 cut(s)	114, 1081, 1416
TauI	GCSGC	2 cut(s)	657, 1943
TfiI	GAWTC	4 cut(s)	94, 781, 1009, 1537
Tru1I	TTAA	4 cut(s)	387, 588, 1769, 2147
Tru9I	TTAA	4 cut(s)	387, 588, 1769, 2147
TscAI	CASTG	5 cut(s)	841, 1249, 1371, 1405, 1431
TseFI	GTSAC	6 cut(s)	193, 241, 361, 1147, 1244, 1321
TseI	GCWGC	6 cut(s)	100, 941, 1184, 1311, 1611, 1932
Tsp45I	GTSAC	6 cut(s)	193, 241, 361, 1147, 1244, 1321
TspDTI	ATGAA	3 cut(s)	403, 1694, 2279
TspGWI	ACGGA	2 cut(s)	583, 2141
TspMI	CCCGGG	1 cut(s)	1860
TspRI	CASTG	5 cut(s)	841, 1249, 1371, 1405, 1431
Tth111I	GACNNNGTC	1 cut(s)	1598
Van91I	CCANNNNNTGG	3 cut(s)	274, 1274, 1928
Vha464I	CTTAAG	1 cut(s)	386
VneI	GTGCAC	1 cut(s)	199
VpaK11BI	GGWCC	4 cut(s)	391, 776, 1265, 1339
XapI	RAATTY	5 cut(s)	337, 705, 1771, 2136, 2142
XceI	RCATGY	2 cut(s)	235, 1736
XcmI	CCANNNNNNNNNTGG	2 cut(s)	268, 2022
XhoI	CTCGAG	1 cut(s)	1358
XmaI	CCCGGG	1 cut(s)	1860
XmiI	GTMKAC	1 cut(s)	1107
XmnI	GAANNNNTTC	1 cut(s)	341
XspI	CTAG	5 cut(s)	912, 1755, 1848, 2235, 2285
ZrmI	AGTACT	1 cut(s)	116
Zsp2I	ATGCAT	1 cut(s)	1199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.