Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0011220

Physical Location & Seq

Genomic Coordinates Reverse (-)

60273714 .. 60277985

Gene Structure 4272 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00210020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGAGCTCCAAAACCCAAAATCTCTGTTTCTGGTGGAGGCTCATCTCCCTTTTGCTTCTTCTAGTACCCTCGTTGTCTCTAAACTCAGATGGGGTTTTCTTGCTTTCTTTCAAGTACTCCGTTCTCAGTGATCCACTTTCAGTCTTGGAAAGCTGGAACTATGAGGATGAAACACCTTGTTCATGGAGTGGAGTCACATGCACCCAACTAGGAAGCCCAGGAACCATTGACATGTTCAGAGTCACAAGCTTGGTCCTCCCAAACAGTCAGCTTCTGGGTTCTATAGCTGAAGAGTTGGGAATGATTGAACACCTCGTCCATCTTGATCTTTCCAACAATTTCTTCAATGGGTCTCTGCCTAAAACCATTCTCAACGCCTCTGAACTTCAGGTGCTTTCTCTCTCAAACAATGTCATCTCCGGTGAGCTTCCTGAGTCCATCGGTGATCTCAAAAGCCTTCAGCTTCTCAACATTTCTGACAATGCTTTGGCCGGAAAAGTCCCTCAAAATCTCACTTCTCTGCAAAACCTCACAGTGGTTTCTTTGAGAAGCAACTACTTCACTGGTAATGTTCCAACCAGGTTTAATTCCGTTGAGGTTTTAGATCTCTCTTCAAATCTACTCAATGGCTCTCTGCCTCTTGATTTTGGTGGAGACAATCTGCGGTACTTAAACATCTCTTACAACAAGATTTGGGGGAAAATACCACCAGAGTTTGCAAGGAATATTTCAGTGAATTCAACCATTGATCTCTCCTTCAACAACTTAACCGGAGCAATCCCGGATTCGCAAGCTTTGCTCAACCAGAAAACAGAGCAGTTTGCAGGAAATGGTGAGCTTTGTGGCAAACCGTTGAAGAATCTTTGCGCAATTCCTTCGACTCTGTCTACTCCACCGAATGTGACTTCATCTTCTCCGGCCATTGCAGCCATTCCCAAAACACTCGACTCACCAGAATCAAACCCTTCCGGCGCACCAAATAGTACTCAGAACAAAACCCAAACCGGGCTAAAACCGGTCACAATCACGGGAATAGTAGTCGGAGACTTAGCCGGAATAGCCATCGTTGGACTGGTCATCCTTTTCGTCTACCAAGTCAGGGAGAAATCAAAGAACAACCCAACTTCCACTTCATCAGACGCAGAGAAGAAACCAGACATTGTCACTAAACTCGACCCAGCTGCAACAAAACCATCCTCATGGTCATGCCTCACCATCAAAGGTGAAGAGACCTCGGAAGCAACGAGCTCAGACAGCGACCACGACGAGAAGCCAAACCATGAAACAGGGGGTGTGCATAGTCAAAACAGCGAAAACTCTCAAAAGCGTGGTGTGCTTATAACCGTGGACGGCGAACCAGAGCTTGAGCTCGAAACGTTGCTGAAGGCCTCGGCGTATGTGTTGGGTGCGAGTGGTCCGAGCATTGTGTACAAGGCGGTGCTGGAAGACAAGACGGCGCTTGCGGTGAGGAGGATCGGAGAGAGTGTGGTGGAGAAGATGAGGGATTTCGAGAATCAAGTGAGGGCCATTGCGAAGATGAGGCACCCAAATTTGGTGAGAGTTCGTGGGTTTTATTGGGGAGAAGATGAGAAGCTTGTGATATATGACTATATCTCCAATGGCAGCCTCGCCGGAACCATTAACAGAAAAGCAGGTTCATCTCCTTACCATCTGCCTCTAGAAGTCCGGTTGAAGATAGCGAAAGGCGTTGCCCGGGGGCTAAGCTACATCCACGAGAAGAAAAACGTGCATGGAAATATAAAACCAAGCAACATTCTGCTAAACTCCGATTTCGACCCTGTAATCAGCGATTTCGGCCTTGACAAGCTAATGTTAGGCGGCATGATCAGTCAAAAAGGTAGCGGTTCTGCCCGAGGGTATTTCGGAAGCCTAAAGTCCGCGGGGGCTCGCGAAGGTATTAATGAAATGCTACCGCCTGTTGCCGGCAGCCCCATCGCGACATCCTCCTCTGCGGGAGGAGCATTGTCGCCTTATCATGCCCCGGAGTCCTTGAAGAACTTGAAGCCGAACCCCAAGTGGGACGTGTACTCTTTTGGCATAGTTTTGCTAGAGCTTCTCACGGGTAGGGTATTTACGGAAAGGGAGCTGGCGGACCAGTGGACCGCCGGTTCACTGATGGGGGAGAAGAACCGGGTTTTGGTAATGGTCGACGTGGCGATTAGGGCTGAGGTGGAGGGCAGGGAAGATGCCTTGCAGGCGTGTTTGAAGCTAGGGTTTAGTTGTGCTAGTTTTGTTCCGCAGAAAAGACCAACCATGAAAGAAGCCCTCCAGGTGCTAGAAAAAAGTATTTCTTCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0007154 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010029 GO:0010030 GO:0010035 GO:0010646 GO:0010647 GO:0016020 GO:0023051 GO:0023056 GO:0031668 GO:0033554 GO:0042221 GO:0042631 GO:0044464 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071944 GO:0104004 GO:1900140 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1905957 GO:1905959 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

773

Amino Acids

83.4

Weight (kDa)

5.85

Isoelectric Point (pI)

33.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	27 - 68	2.2e-13	Leucine rich repeat N-terminal domain
LRR_14	PF23598	95 - 184	4.6e-10	Leucine-rich repeat region
LRR_8	PF13855	104 - 163	5e-07	Leucine rich repeat
Pkinase	PF00069	468 - 611	1.2e-18	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	474 - 611	2.7e-19	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	664 - 764	3.5e-08	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011220)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15300 AT4G34220
fragaria_vesca	FvH4_2g05270
malus_domestica	MD05G1088500.v1.1 MD10G1096400.v1.1
prunus_persica	Prupe.8G134500_v2.0.a1
pyrus_communis	pycom05g08790 pycom10g08270
rosa_chinensis	RchiOBHm_Chr6g0255401
rosa_laevigata	RLG00000014841
rosa_multiflora	Rmu_co8383769.1_g000001 Rmu_sc0002360.1_g000015
rosa_roxburghii	Rroxscaffold_7G00210020
rosa_rugosa	Rorug05G0558100.1 Rorug05G0558200
rosa_samantha	Rh6AG075300 Rh6BG068400 Rh6CG068000 Rh6DG064700
rosa_wichuraiana	Rw6G006620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1340
Acc16I	TGCGCA	1 cut(s)	868
Acc36I	ACCTGC	1 cut(s)	1643
AccB1I	GGYRCC	1 cut(s)	1542
AccI	GTMKAC	3 cut(s)	887, 1089, 2169
AccII	CGCG	3 cut(s)	1901, 1911, 1958
AclI	AACGTT	1 cut(s)	1376
AclWI	GGATC	2 cut(s)	125, 1481
AcoI	YGGCCR	2 cut(s)	489, 918
AcsI	RAATTY	2 cut(s)	736, 1549
AcuI	CTGAAG	4 cut(s)	309, 371, 443, 1403
AfaI	GTAC	6 cut(s)	66, 116, 668, 985, 1430, 2048
AfiI	CCNNNNNNNGG	7 cut(s)	535, 1005, 1099, 1552, 1943, 2038, 2158
AflIII	ACRYGT	2 cut(s)	231, 2043
AgeI	ACCGGT	1 cut(s)	1015
AjiI	CACGTC	2 cut(s)	2044, 2173
AjnI	CCWGG	3 cut(s)	217, 578, 2289
AjuI	GAANNNNNNNTTGG	6 cut(s)	326, 358, 1114, 1146, 2141, 2173
AloI	GAACNNNNNNTCC	4 cut(s)	300, 332, 2113, 2145
Alw21I	GWGCWC	3 cut(s)	8, 1250, 1371
Alw26I	GTCTC	5 cut(s)	81, 357, 648, 1038, 1223
AlwI	GGATC	2 cut(s)	125, 1481
AlwNI	CAGNNNCTG	1 cut(s)	274
Ama87I	CYCGRG	2 cut(s)	1713, 1872
AoxI	GGCC	5 cut(s)	489, 918, 1386, 1524, 1816
ApeKI	GCWGC	4 cut(s)	926, 1181, 1623, 1947
ApoI	RAATTY	2 cut(s)	736, 1549
AseI	ATTAAT	1 cut(s)	1920
AsiGI	ACCGGT	1 cut(s)	1015
AspLEI	GCGC	3 cut(s)	869, 974, 1459
AspS9I	GGNCC	5 cut(s)	253, 1415, 1524, 2113, 2121
AsuC2I	CCSGG	6 cut(s)	782, 1006, 1714, 1715, 2003, 2153
AsuHPI	GGTGA	8 cut(s)	434, 455, 845, 942, 1204, 1235, 1477, 1567
AvaI	CYCGRG	2 cut(s)	1713, 1872
AvaII	GGWCC	4 cut(s)	253, 1415, 2113, 2121
BanI	GGYRCC	1 cut(s)	1542
BanII	GRGCYC	4 cut(s)	8, 1250, 1371, 1909
BauI	CACGAG	1 cut(s)	1733
BbsI	GAAGAC	1 cut(s)	1452
Bbv12I	GWGCWC	3 cut(s)	8, 1250, 1371
BbvCI	CCTCAGC	1 cut(s)	2187
BbvI	GCAGC	4 cut(s)	938, 1168, 1635, 1959
BccI	CCATC	9 cut(s)	83, 327, 446, 1070, 1201, 1223, 1677, 1961, 2131
BceAI	ACGGC	2 cut(s)	1366, 1470
BcgI	CGANNNNNNTGC	6 cut(s)	858, 892, 1163, 1197, 1936, 1970
BciT130I	CCWGG	3 cut(s)	219, 580, 2291
BclI	TGATCA	1 cut(s)	1845
BcnI	CCSGG	6 cut(s)	782, 1006, 1714, 1715, 2003, 2153
BcoDI	GTCTC	5 cut(s)	81, 357, 648, 1038, 1223
BfaI	CTAG	7 cut(s)	62, 209, 1679, 2069, 2231, 2247, 2297
BfmI	CTRYAG	1 cut(s)	282
BfoI	RGCGCY	1 cut(s)	1460
BfuAI	ACCTGC	1 cut(s)	1643
BglI	GCCNNNNNGGC	1 cut(s)	2216
BglII	AGATCT	1 cut(s)	604
BisI	GCNGC	5 cut(s)	927, 1182, 1624, 1840, 1948
BlpI	GCTNAGC	1 cut(s)	1721
BlsI	GCNGC	5 cut(s)	928, 1183, 1625, 1841, 1949
BmcAI	AGTACT	2 cut(s)	116, 985
Bme1390I	CCNGG	9 cut(s)	219, 580, 782, 1006, 1714, 1715, 2003, 2153, 2291
Bme18I	GGWCC	4 cut(s)	253, 1415, 2113, 2121
BmeT110I	CYCGRG	2 cut(s)	1713, 1872
BmgBI	CACGTC	2 cut(s)	2044, 2173
BmgT120I	GGNCC	5 cut(s)	253, 1415, 1524, 2113, 2121
BmiI	GGNNCC	3 cut(s)	223, 1544, 1636
BmrFI	CCNGG	9 cut(s)	219, 580, 782, 1006, 1714, 1715, 2003, 2153, 2291
BmsI	GCATC	1 cut(s)	2197
BpiI	GAAGAC	1 cut(s)	1452
BpmI	CTGGAG	1 cut(s)	2273
Bpu10I	CCTNAGC	1 cut(s)	2187
Bpu1102I	GCTNAGC	1 cut(s)	1721
BpuEI	CTTGAG	1 cut(s)	1385
BpuMI	CCSGG	6 cut(s)	782, 1006, 1714, 1715, 2003, 2153
BsaI	GGTCTC	2 cut(s)	357, 1223
BsaJI	CCNNGG	9 cut(s)	217, 1233, 1344, 1389, 1713, 1714, 1873, 1899, 2001
BsaWI	WCCGGW	4 cut(s)	419, 770, 1015, 1686
BsaXI	ACNNNNNCTCC	4 cut(s)	1470, 1500, 1598, 1628
Bsc4I	CCNNNNNNNGG	7 cut(s)	535, 1005, 1099, 1552, 1943, 2038, 2158
Bse118I	RCCGGY	3 cut(s)	1015, 1943, 2126
Bse1I	ACTGG	3 cut(s)	568, 1077, 2116
Bse3DI	GCAATG	2 cut(s)	921, 1527
BseBI	CCWGG	3 cut(s)	219, 580, 2291
BseDI	CCNNGG	9 cut(s)	217, 1233, 1344, 1389, 1713, 1714, 1873, 1899, 2001
BseGI	GGATG	5 cut(s)	172, 1077, 1193, 1728, 1961
BseLI	CCNNNNNNNGG	7 cut(s)	535, 1005, 1099, 1552, 1943, 2038, 2158
BseMI	GCAATG	2 cut(s)	921, 1527
BseMII	CTCAG	6 cut(s)	99, 139, 423, 1001, 1263, 2178
BseNI	ACTGG	3 cut(s)	568, 1077, 2116
BseRI	GAGGAG	3 cut(s)	1483, 1957, 1992
BseXI	GCAGC	4 cut(s)	938, 1168, 1635, 1959
BseYI	CCCAGC	1 cut(s)	1177
Bsh1236I	CGCG	3 cut(s)	1901, 1911, 1958
BshFI	GGCC	5 cut(s)	491, 920, 1388, 1526, 1818
BshNI	GGYRCC	1 cut(s)	1542
BshTI	ACCGGT	1 cut(s)	1015
BsiHKAI	GWGCWC	3 cut(s)	8, 1250, 1371
BsiHKCI	CYCGRG	2 cut(s)	1713, 1872
BslFI	GGGAC	2 cut(s)	485, 2054
BslI	CCNNNNNNNGG	7 cut(s)	535, 1005, 1099, 1552, 1943, 2038, 2158
BsmAI	GTCTC	5 cut(s)	81, 357, 648, 1038, 1223
BsmFI	GGGAC	2 cut(s)	485, 2054
BsnI	GGCC	5 cut(s)	491, 920, 1388, 1526, 1818
Bso31I	GGTCTC	2 cut(s)	357, 1223
BsoBI	CYCGRG	2 cut(s)	1713, 1872
Bsp1286I	GDGCHC	4 cut(s)	8, 1250, 1371, 1909
Bsp1407I	TGTACA	1 cut(s)	1428
Bsp143I	GATC	7 cut(s)	130, 325, 445, 604, 748, 1473, 1845
Bsp1720I	GCTNAGC	1 cut(s)	1721
Bsp68I	TCGCGA	2 cut(s)	1911, 1958
BspANI	GGCC	5 cut(s)	491, 920, 1388, 1526, 1818
BspCNI	CTCAG	6 cut(s)	98, 138, 424, 1000, 1262, 2179
BspFNI	CGCG	3 cut(s)	1901, 1911, 1958
BspLI	GGNNCC	3 cut(s)	223, 1544, 1636
BspMI	ACCTGC	1 cut(s)	1643
BspPI	GGATC	2 cut(s)	125, 1481
BspT107I	GGYRCC	1 cut(s)	1542
BspTNI	GGTCTC	2 cut(s)	357, 1223
BsrDI	GCAATG	2 cut(s)	921, 1527
BsrFI	RCCGGY	3 cut(s)	1015, 1943, 2126
BsrGI	TGTACA	1 cut(s)	1428
BsrI	ACTGG	3 cut(s)	568, 1077, 2116
BssAI	RCCGGY	3 cut(s)	1015, 1943, 2126
BssECI	CCNNGG	9 cut(s)	217, 1233, 1344, 1389, 1713, 1714, 1873, 1899, 2001
BssMI	GATC	7 cut(s)	130, 325, 445, 604, 748, 1473, 1845
BssSI	CACGAG	1 cut(s)	1733
Bst2BI	CACGAG	1 cut(s)	1733
Bst2UI	CCWGG	3 cut(s)	219, 580, 2291
Bst4CI	ACNGT	4 cut(s)	266, 535, 852, 1345
Bst6I	CTCTTC	3 cut(s)	285, 616, 1221
BstAPI	GCANNNNNTGC	1 cut(s)	796
BstAUI	TGTACA	1 cut(s)	1428
BstC8I	GCNNGC	6 cut(s)	792, 1461, 1909, 1945, 2109, 2217
BstDEI	CTNAG	8 cut(s)	85, 125, 432, 987, 1048, 1249, 1721, 2187
BstDSI	CCRYGG	2 cut(s)	1344, 1899
BstF5I	GGATG	5 cut(s)	172, 1077, 1193, 1728, 1961
BstFNI	CGCG	3 cut(s)	1901, 1911, 1958
BstH2I	RGCGCY	1 cut(s)	1460
BstHHI	GCGC	3 cut(s)	869, 974, 1459
BstKTI	GATC	7 cut(s)	133, 328, 448, 607, 751, 1476, 1848
BstMAI	GTCTC	5 cut(s)	81, 357, 648, 1038, 1223
BstMBI	GATC	7 cut(s)	130, 325, 445, 604, 748, 1473, 1845
BstMWI	GCNNNNNNNGC	9 cut(s)	796, 926, 1058, 1254, 1333, 1815, 1979, 2183, 2216
BstNI	CCWGG	3 cut(s)	219, 580, 2291
BstNSI	RCATGY	2 cut(s)	201, 235
BstSCI	CCNGG	9 cut(s)	217, 578, 780, 1004, 1712, 1713, 2001, 2151, 2289
BstSFI	CTRYAG	1 cut(s)	282
BstUI	CGCG	3 cut(s)	1901, 1911, 1958
BstV1I	GCAGC	4 cut(s)	938, 1168, 1635, 1959
BstV2I	GAAGAC	1 cut(s)	1452
BstX2I	RGATCY	1 cut(s)	604
BstXI	CCANNNNNNTGG	1 cut(s)	1200
BstYI	RGATCY	1 cut(s)	604
BsuRI	GGCC	5 cut(s)	491, 920, 1388, 1526, 1818
BtgI	CCRYGG	2 cut(s)	1344, 1899
BtgZI	GCGATG	1 cut(s)	1939
BtrI	CACGTC	2 cut(s)	2044, 2173
BtsCI	GGATG	5 cut(s)	172, 1077, 1193, 1728, 1961
BtsIMutI	CAGTG	6 cut(s)	133, 540, 561, 738, 2123, 2132
BtuMI	TCGCGA	2 cut(s)	1911, 1958
BveI	ACCTGC	1 cut(s)	1643
Cac8I	GCNNGC	6 cut(s)	792, 1461, 1909, 1945, 2109, 2217
CaiI	CAGNNNCTG	1 cut(s)	274
CfoI	GCGC	3 cut(s)	869, 974, 1459
Cfr10I	RCCGGY	3 cut(s)	1015, 1943, 2126
Cfr13I	GGNCC	5 cut(s)	253, 1415, 1524, 2113, 2121
Cfr42I	CCGCGG	1 cut(s)	1902
Cfr9I	CCCGGG	1 cut(s)	1713
CseI	GACGC	1 cut(s)	1148
CsiI	ACCWGGT	1 cut(s)	578
Csp6I	GTAC	6 cut(s)	65, 115, 667, 984, 1429, 2047
CspAI	ACCGGT	1 cut(s)	1015
CviQI	GTAC	6 cut(s)	65, 115, 667, 984, 1429, 2047
DdeI	CTNAG	8 cut(s)	85, 125, 432, 987, 1048, 1249, 1721, 2187
DpnI	GATC	7 cut(s)	132, 327, 447, 606, 750, 1475, 1847
DpnII	GATC	7 cut(s)	130, 325, 445, 604, 748, 1473, 1845
EaeI	YGGCCR	2 cut(s)	489, 918
Eam1104I	CTCTTC	3 cut(s)	285, 616, 1221
EarI	CTCTTC	3 cut(s)	285, 616, 1221
EciI	GGCGGA	1 cut(s)	2126
Ecl136II	GAGCTC	3 cut(s)	6, 1248, 1369
Eco147I	AGGCCT	1 cut(s)	1388
Eco24I	GRGCYC	4 cut(s)	8, 1250, 1371, 1909
Eco31I	GGTCTC	2 cut(s)	357, 1223
Eco47I	GGWCC	4 cut(s)	253, 1415, 2113, 2121
Eco53kI	GAGCTC	3 cut(s)	6, 1248, 1369
Eco57I	CTGAAG	4 cut(s)	309, 371, 443, 1403
Eco88I	CYCGRG	2 cut(s)	1713, 1872
EcoICRI	GAGCTC	3 cut(s)	6, 1248, 1369
EcoRI	GAATTC	1 cut(s)	736
EcoRII	CCWGG	3 cut(s)	217, 578, 2289
EcoT38I	GRGCYC	4 cut(s)	8, 1250, 1371, 1909
FalI	AAGNNNNNCTT	1 cut(s)	2296
FaqI	GGGAC	2 cut(s)	485, 2054
FauI	CCCGC	2 cut(s)	1894, 1966
FbaI	TGATCA	1 cut(s)	1845
FblI	GTMKAC	3 cut(s)	887, 1089, 2169
Fnu4HI	GCNGC	5 cut(s)	927, 1182, 1624, 1840, 1948
FokI	GGATG	5 cut(s)	179, 1064, 1180, 1715, 1948
FriOI	GRGCYC	4 cut(s)	8, 1250, 1371, 1909
Fsp4HI	GCNGC	5 cut(s)	927, 1182, 1624, 1840, 1948
FspBI	CTAG	7 cut(s)	62, 209, 1679, 2069, 2231, 2247, 2297
FspI	TGCGCA	1 cut(s)	868
GlaI	GCGC	3 cut(s)	868, 973, 1458
GluI	GCNGC	5 cut(s)	927, 1182, 1624, 1840, 1948
GsaI	CCCAGC	1 cut(s)	1181
GsuI	CTGGAG	1 cut(s)	2273
HaeII	RGCGCY	1 cut(s)	1460
HaeIII	GGCC	5 cut(s)	491, 920, 1388, 1526, 1818
HgaI	GACGC	1 cut(s)	1148
HhaI	GCGC	3 cut(s)	869, 974, 1459
Hin6I	GCGC	3 cut(s)	867, 972, 1457
HinP1I	GCGC	3 cut(s)	867, 972, 1457
HincII	GTYRAC	1 cut(s)	2170
HindII	GTYRAC	1 cut(s)	2170
HindIII	AAGCTT	3 cut(s)	247, 792, 1592
HphI	GGTGA	8 cut(s)	434, 455, 845, 942, 1204, 1235, 1477, 1567
Hpy166II	GTNNAC	8 cut(s)	888, 1090, 1348, 1429, 2047, 2121, 2132, 2170
Hpy188III	TCNNGA	7 cut(s)	323, 431, 641, 1510, 1679, 1910, 1957
Hpy8I	GTNNAC	8 cut(s)	888, 1090, 1348, 1429, 2047, 2121, 2132, 2170
Hpy99I	CGWCG	2 cut(s)	1268, 2174
HpyAV	CCTTC	6 cut(s)	467, 766, 885, 975, 1378, 1907
HpyCH4III	ACNGT	4 cut(s)	266, 535, 852, 1345
HpyCH4IV	ACGT	4 cut(s)	1376, 1746, 2043, 2172
HpyCH4V	TGCA	9 cut(s)	201, 523, 719, 824, 926, 1184, 1297, 1750, 2215
HpyF10VI	GCNNNNNNNGC	9 cut(s)	796, 926, 1058, 1254, 1333, 1815, 1979, 2183, 2216
HpyF3I	CTNAG	8 cut(s)	85, 125, 432, 987, 1048, 1249, 1721, 2187
HpySE526I	ACGT	4 cut(s)	1376, 1746, 2043, 2172
HspAI	GCGC	3 cut(s)	867, 972, 1457
KroI	GCCGGC	1 cut(s)	1943
KroNI	GCCGGC	1 cut(s)	1945
Ksp22I	TGATCA	1 cut(s)	1845
KspI	CCGCGG	1 cut(s)	1902
Kzo9I	GATC	7 cut(s)	130, 325, 445, 604, 748, 1473, 1845
LmnI	GCTCC	4 cut(s)	11, 773, 1979, 2104
Lsp1109I	GCAGC	4 cut(s)	938, 1168, 1635, 1959
LweI	GCATC	1 cut(s)	2197
MabI	ACCWGGT	1 cut(s)	578
MaeI	CTAG	7 cut(s)	62, 209, 1679, 2069, 2231, 2247, 2297
MaeII	ACGT	4 cut(s)	1376, 1746, 2043, 2172
MaeIII	GTNAC	5 cut(s)	193, 241, 901, 1018, 1162
MalI	GATC	7 cut(s)	132, 327, 447, 606, 750, 1475, 1847
MboI	GATC	7 cut(s)	130, 325, 445, 604, 748, 1473, 1845
MflI	RGATCY	1 cut(s)	604
MhlI	GDGCHC	4 cut(s)	8, 1250, 1371, 1909
MluCI	AATT	5 cut(s)	337, 586, 736, 870, 1549
MlyI	GAGTC	6 cut(s)	201, 249, 443, 874, 941, 2015
MmeI	TCCRAC	4 cut(s)	357, 599, 1021, 1048
MroNI	GCCGGC	1 cut(s)	1943
MseI	TTAA	5 cut(s)	585, 671, 767, 1641, 1920
MslI	CAYNNNNRTG	3 cut(s)	230, 1198, 1204
MspA1I	CMGCKG	2 cut(s)	1181, 1901
MspR9I	CCNGG	9 cut(s)	219, 580, 782, 1006, 1714, 1715, 2003, 2153, 2291
MvaI	CCWGG	3 cut(s)	219, 580, 2291
MvnI	CGCG	3 cut(s)	1901, 1911, 1958
MwoI	GCNNNNNNNGC	9 cut(s)	796, 926, 1058, 1254, 1333, 1815, 1979, 2183, 2216
NaeI	GCCGGC	1 cut(s)	1945
NciI	CCSGG	6 cut(s)	782, 1006, 1714, 1715, 2003, 2153
NdeII	GATC	7 cut(s)	130, 325, 445, 604, 748, 1473, 1845
NgoMIV	GCCGGC	1 cut(s)	1943
NlaIV	GGNNCC	3 cut(s)	223, 1544, 1636
NmeAIII	GCCGAG	1 cut(s)	1370
NmuCI	GTSAC	5 cut(s)	193, 241, 901, 1018, 1162
NruI	TCGCGA	2 cut(s)	1911, 1958
NsbI	TGCGCA	1 cut(s)	868
NspI	RCATGY	2 cut(s)	201, 235
PceI	AGGCCT	1 cut(s)	1388
PciI	ACATGT	1 cut(s)	231
PcsI	WCGNNNNNNNCGW	1 cut(s)	1241
PdiI	GCCGGC	1 cut(s)	1945
PfeI	GAWTC	4 cut(s)	785, 859, 956, 1513
PflFI	GACNNNGTC	2 cut(s)	883, 1160
PfoI	TCCNGGA	1 cut(s)	780
PinAI	ACCGGT	1 cut(s)	1015
PkrI	GCNGC	5 cut(s)	928, 1183, 1625, 1841, 1949
PleI	GAGTC	6 cut(s)	200, 248, 442, 874, 941, 2014
PpsI	GAGTC	6 cut(s)	200, 248, 442, 874, 941, 2014
PscI	ACATGT	1 cut(s)	231
PshBI	ATTAAT	1 cut(s)	1920
PsiI	TTATAA	1 cut(s)	1340
Psp124BI	GAGCTC	3 cut(s)	8, 1250, 1371
Psp1406I	AACGTT	1 cut(s)	1376
Psp6I	CCWGG	3 cut(s)	217, 578, 2289
PspFI	CCCAGC	1 cut(s)	1177
PspGI	CCWGG	3 cut(s)	217, 578, 2289
PspN4I	GGNNCC	3 cut(s)	223, 1544, 1636
PspPI	GGNCC	5 cut(s)	253, 1415, 1524, 2113, 2121
PstNI	CAGNNNCTG	1 cut(s)	274
PsuI	RGATCY	1 cut(s)	604
PsyI	GACNNNGTC	2 cut(s)	883, 1160
PvuII	CAGCTG	1 cut(s)	1181
RruI	TCGCGA	2 cut(s)	1911, 1958
RsaI	GTAC	6 cut(s)	66, 116, 668, 985, 1430, 2048
RsaNI	GTAC	6 cut(s)	65, 115, 667, 984, 1429, 2047
RseI	CAYNNNNRTG	3 cut(s)	230, 1198, 1204
SacI	GAGCTC	3 cut(s)	8, 1250, 1371
SacII	CCGCGG	1 cut(s)	1902
SalI	GTCGAC	1 cut(s)	2168
SaqAI	TTAA	5 cut(s)	585, 671, 767, 1641, 1920
SatI	GCNGC	5 cut(s)	927, 1182, 1624, 1840, 1948
Sau3AI	GATC	7 cut(s)	130, 325, 445, 604, 748, 1473, 1845
Sau96I	GGNCC	5 cut(s)	253, 1415, 1524, 2113, 2121
ScaI	AGTACT	2 cut(s)	116, 985
SchI	GAGTC	6 cut(s)	201, 249, 443, 874, 941, 2015
ScrFI	CCNGG	9 cut(s)	219, 580, 782, 1006, 1714, 1715, 2003, 2153, 2291
SduI	GDGCHC	4 cut(s)	8, 1250, 1371, 1909
SexAI	ACCWGGT	1 cut(s)	578
SfaNI	GCATC	1 cut(s)	2197
SfcI	CTRYAG	1 cut(s)	282
Sfr303I	CCGCGG	1 cut(s)	1902
SgrBI	CCGCGG	1 cut(s)	1902
SinI	GGWCC	4 cut(s)	253, 1415, 2113, 2121
SmaI	CCCGGG	1 cut(s)	1715
SmiMI	CAYNNNNRTG	3 cut(s)	230, 1198, 1204
SmlI	CTYRAG	1 cut(s)	1364
SmoI	CTYRAG	1 cut(s)	1364
Sse9I	AATT	5 cut(s)	337, 586, 736, 870, 1549
SseBI	AGGCCT	1 cut(s)	1388
SspI	AATATT	1 cut(s)	727
SspMI	CTAG	7 cut(s)	62, 209, 1679, 2069, 2231, 2247, 2297
SstI	GAGCTC	3 cut(s)	8, 1250, 1371
StuI	AGGCCT	1 cut(s)	1388
StyD4I	CCNGG	9 cut(s)	217, 578, 780, 1004, 1712, 1713, 2001, 2151, 2289
TaaI	ACNGT	4 cut(s)	266, 535, 852, 1345
TaiI	ACGT	4 cut(s)	1379, 1749, 2046, 2175
TaqI	TCGA	7 cut(s)	878, 945, 1173, 1371, 1509, 1794, 2169
TasI	AATT	5 cut(s)	337, 586, 736, 870, 1549
TatI	WGTACW	4 cut(s)	114, 983, 1428, 2046
TauI	GCSGC	1 cut(s)	1842
TfiI	GAWTC	4 cut(s)	785, 859, 956, 1513
Tru1I	TTAA	5 cut(s)	585, 671, 767, 1641, 1920
Tru9I	TTAA	5 cut(s)	585, 671, 767, 1641, 1920
TscAI	CASTG	6 cut(s)	133, 540, 568, 738, 2123, 2139
TseFI	GTSAC	5 cut(s)	193, 241, 901, 1018, 1162
TseI	GCWGC	4 cut(s)	926, 1181, 1623, 1947
Tsp45I	GTSAC	5 cut(s)	193, 241, 901, 1018, 1162
TspDTI	ATGAA	8 cut(s)	171, 183, 897, 1122, 1296, 1647, 1938, 2291
TspGWI	ACGGA	3 cut(s)	109, 580, 2111
TspMI	CCCGGG	1 cut(s)	1713
TspRI	CASTG	6 cut(s)	133, 540, 568, 738, 2123, 2139
Tth111I	GACNNNGTC	2 cut(s)	883, 1160
VpaK11BI	GGWCC	4 cut(s)	253, 1415, 2113, 2121
VspI	ATTAAT	1 cut(s)	1920
XapI	RAATTY	2 cut(s)	736, 1549
XbaI	TCTAGA	1 cut(s)	1678
XceI	RCATGY	2 cut(s)	201, 235
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1069
XmaI	CCCGGG	1 cut(s)	1713
XmiI	GTMKAC	3 cut(s)	887, 1089, 2169
XspI	CTAG	7 cut(s)	62, 209, 1679, 2069, 2231, 2247, 2297
ZrmI	AGTACT	2 cut(s)	116, 985

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.