MD05G1114500.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014818
Physical Location & Seq
Reverse (-)
23317235 .. 23318478
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1114500.v1.1.491

Sequence Viewer

Length: 915 bp
ATGGCGGAGTTTGTCATAATCCAAACTGAAGCTGATCAAAAATTGCCCAAATCAGTTGGAGGAAGTTGTGACACAATCGGTGCCCGAAATTCCTTGGGGCCAAAGTTTCTTGCTGCAATTGGTGCCCATGAATACTTTTCACCAGAGATGTGGAGAGCAGCTCTGACAGAATTAGTAGCAACCGCTTCTCTAGTATTCAGCCTAACCTCTTCCATCATATCATGCTTGGATTCAGGTGAAGTTGATCCGAAACTGCTTGTCCCCATTTCCGTCTTCATCATAGCCTTCCTCCTTCTCCTTGTGACAGTTCCGTTATCGGGCGGCCACATGAGCCCAGTTTTCACATTTATAGCTACCCTCAAGGGTGCCATAACCCTAGCTCGTGCTTCCATCTACATTTTGGCACAATGTTTTGGCTCAGTTTTAGGGTTTCTCATAATGAAGACTGTGATGGACCAACATGCAGTACAAAAGTACTCCTTAGGTGGCTGCACAATCCAAGGAAGTGGGCCCACATCTGGGGTGGAGACACAAACAGCTTTGGTACTAGAAGTTACTTGCACTTTTGTGGTTCTCTTTGTTGGAGTCACAGTGGCATTTGACAAGAGAAGAAGCAAAGAATTAGGGTTACCAATGGTGTGCGTTGTGGTGGCTGGGTCTATGGCAATTGCAGTTTTTGTGTCGGTAACGGTAACCGGGCGTGCTGGGTACGCCGGGGTAGGACTGAATCCGGCAAGGTGCTTAGGGGCAGCACTGTTGCAAGGAGGGATATTGTGGGATGGTCATTGGGTTTTTTGGGTTGGACCAATTTTGGCTTGTAGTGTGTACCATTGTTTGTCCTTGAACTTGCCAAAGCAGGATTTAACGTCAGTAGAAGAAGAGTGTGAGATTATGAAAGGAGAAAGAAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko04962 ko04976 map04962 map04976
Pfam Domains
Protein Families
MIP

Protein Analysis

305

Amino Acids

32.17

Weight (kDa)

5.96

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 47 - 271 5.2e-31 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 80, 122, 365
AciI CCGC 3 cut(s) 5, 183, 321
AclWI GGATC 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 5 cut(s) 468, 476, 546, 710, 827
AfiI CCNNNNNNNGG 3 cut(s) 317, 518, 519
AgsI TTSAA 1 cut(s) 844
AleI CACNNNNGTG 1 cut(s) 566
AluBI AGCT 5 cut(s) 32, 161, 353, 380, 539
AluI AGCT 5 cut(s) 32, 161, 353, 380, 539
Alw26I GTCTC 1 cut(s) 521
AlwI GGATC 1 cut(s) 239
AoxI GGCC 3 cut(s) 98, 322, 509
ApaI GGGCCC 1 cut(s) 513
ApeKI GCWGC 4 cut(s) 113, 158, 489, 749
ApoI RAATTY 1 cut(s) 88
AspS9I GGNCC 5 cut(s) 98, 454, 509, 510, 803
AsuC2I CCSGG 2 cut(s) 697, 715
AsuHPI GGTGA 2 cut(s) 132, 248
AvaII GGWCC 2 cut(s) 454, 803
AxyI CCTNAGG 1 cut(s) 481
BaeGI GKGCMC 3 cut(s) 85, 127, 513
BanI GGYRCC 3 cut(s) 80, 122, 365
BanII GRGCYC 2 cut(s) 335, 513
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 2 cut(s) 265, 449
BbvI GCAGC 4 cut(s) 100, 170, 476, 761
BccI CCATC 4 cut(s) 221, 398, 445, 773
BclI TGATCA 1 cut(s) 34
BcnI CCSGG 2 cut(s) 697, 715
BcoDI GTCTC 1 cut(s) 521
BfaI CTAG 3 cut(s) 191, 377, 548
BisI GCNGC 5 cut(s) 114, 159, 322, 490, 750
BlsI GCNGC 5 cut(s) 115, 160, 323, 491, 751
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 2 cut(s) 697, 715
Bme18I GGWCC 2 cut(s) 454, 803
BmgT120I GGNCC 5 cut(s) 98, 454, 509, 510, 803
BmiI GGNNCC 5 cut(s) 82, 99, 124, 367, 511
BmrFI CCNGG 2 cut(s) 697, 715
BmrI ACTGGG 1 cut(s) 329
BmuI ACTGGG 1 cut(s) 329
BpiI GAAGAC 2 cut(s) 265, 449
BplI GAGNNNNNCTC 2 cut(s) 145, 177
Bpu10I CCTNAGC 1 cut(s) 742
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 2 cut(s) 697, 715
BsaJI CCNNGG 3 cut(s) 93, 499, 714
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 518, 519
Bse1I ACTGG 1 cut(s) 335
Bse21I CCTNAGG 1 cut(s) 481
BseDI CCNNGG 3 cut(s) 93, 499, 714
BseGI GGATG 1 cut(s) 784
BseLI CCNNNNNNNGG 3 cut(s) 317, 518, 519
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 335
BseSI GKGCMC 3 cut(s) 85, 127, 513
BseXI GCAGC 4 cut(s) 100, 170, 476, 761
BseYI CCCAGC 2 cut(s) 653, 704
BsgI GTGCAG 1 cut(s) 475
BshFI GGCC 3 cut(s) 100, 324, 511
BshNI GGYRCC 3 cut(s) 80, 122, 365
BsiSI CCGG 3 cut(s) 696, 714, 731
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 3 cut(s) 317, 518, 519
BsmAI GTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 3 cut(s) 100, 324, 511
Bsp120I GGGCCC 1 cut(s) 509
Bsp1286I GDGCHC 4 cut(s) 85, 127, 335, 513
Bsp143I GATC 2 cut(s) 34, 244
BspACI CCGC 3 cut(s) 5, 183, 321
BspANI GGCC 3 cut(s) 100, 324, 511
BspCNI CTCAG 1 cut(s) 431
BspLI GGNNCC 5 cut(s) 82, 99, 124, 367, 511
BspPI GGATC 1 cut(s) 239
BspT107I GGYRCC 3 cut(s) 80, 122, 365
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 3 cut(s) 93, 499, 714
BssMI GATC 2 cut(s) 34, 244
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 2 cut(s) 93, 499
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 5 cut(s) 307, 448, 592, 691, 756
Bst6I CTCTTC 2 cut(s) 214, 873
BstAPI GCANNNNNTGC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 702
BstDEI CTNAG 3 cut(s) 418, 481, 742
BstEII GGTNACC 2 cut(s) 627, 691
BstF5I GGATG 1 cut(s) 784
BstKTI GATC 2 cut(s) 37, 247
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 2 cut(s) 34, 244
BstMWI GCNNNNNNNGC 3 cut(s) 122, 330, 710
BstNSI RCATGY 1 cut(s) 464
BstPI GGTNACC 2 cut(s) 627, 691
BstSCI CCNGG 2 cut(s) 695, 713
BstSLI GKGCMC 3 cut(s) 85, 127, 513
BstV1I GCAGC 4 cut(s) 100, 170, 476, 761
BstV2I GAAGAC 2 cut(s) 265, 449
BstXI CCANNNNNNTGG 2 cut(s) 150, 506
Bsu36I CCTNAGG 1 cut(s) 481
BsuRI GGCC 3 cut(s) 100, 324, 511
BtsCI GGATG 1 cut(s) 784
BtsIMutI CAGTG 2 cut(s) 597, 752
Cac8I GCNNGC 1 cut(s) 702
Cfr13I GGNCC 5 cut(s) 98, 454, 509, 510, 803
Csp6I GTAC 5 cut(s) 467, 475, 545, 709, 826
CviAII CATG 4 cut(s) 128, 222, 328, 461
CviQI GTAC 5 cut(s) 467, 475, 545, 709, 826
DdeI CTNAG 3 cut(s) 418, 481, 742
DpnI GATC 2 cut(s) 36, 246
DpnII GATC 2 cut(s) 34, 244
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 214, 873
EarI CTCTTC 2 cut(s) 214, 873
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 93, 499
Eco24I GRGCYC 2 cut(s) 335, 513
Eco47I GGWCC 2 cut(s) 454, 803
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 481
Eco91I GGTNACC 2 cut(s) 627, 691
EcoO65I GGTNACC 2 cut(s) 627, 691
EcoT14I CCWWGG 2 cut(s) 93, 499
EcoT38I GRGCYC 2 cut(s) 335, 513
ErhI CCWWGG 2 cut(s) 93, 499
FaeI CATG 4 cut(s) 131, 225, 331, 464
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 245
FatI CATG 4 cut(s) 127, 221, 327, 460
FbaI TGATCA 1 cut(s) 34
Fnu4HI GCNGC 5 cut(s) 114, 159, 322, 490, 750
FokI GGATG 1 cut(s) 791
FriOI GRGCYC 2 cut(s) 335, 513
Fsp4HI GCNGC 5 cut(s) 114, 159, 322, 490, 750
FspBI CTAG 3 cut(s) 191, 377, 548
GluI GCNGC 5 cut(s) 114, 159, 322, 490, 750
GsaI CCCAGC 2 cut(s) 657, 708
HaeIII GGCC 3 cut(s) 100, 324, 511
HapII CCGG 3 cut(s) 696, 714, 731
Hin1II CATG 4 cut(s) 131, 225, 331, 464
HinfI GANTC 3 cut(s) 230, 585, 727
HpaII CCGG 3 cut(s) 696, 714, 731
HphI GGTGA 2 cut(s) 132, 248
Hpy166II GTNNAC 1 cut(s) 826
Hpy188I TCNGA 2 cut(s) 165, 249
Hpy8I GTNNAC 1 cut(s) 826
HpyAV CCTTC 2 cut(s) 295, 302
HpyCH4III ACNGT 5 cut(s) 307, 448, 592, 691, 756
HpyCH4IV ACGT 1 cut(s) 866
HpyCH4V TGCA 6 cut(s) 116, 464, 492, 561, 671, 760
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 330, 710
HpyF3I CTNAG 3 cut(s) 418, 481, 742
HpySE526I ACGT 1 cut(s) 866
Hsp92II CATG 4 cut(s) 131, 225, 331, 464
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 2 cut(s) 34, 244
Lsp1109I GCAGC 4 cut(s) 100, 170, 476, 761
MaeI CTAG 3 cut(s) 191, 377, 548
MaeII ACGT 1 cut(s) 866
MaeIII GTNAC 7 cut(s) 68, 301, 553, 586, 627, 685, 691
MalI GATC 2 cut(s) 36, 246
MboI GATC 2 cut(s) 34, 244
MboII GAAGA 6 cut(s) 201, 265, 454, 621, 887, 890
MfeI CAATTG 2 cut(s) 117, 666
MhlI GDGCHC 4 cut(s) 85, 127, 335, 513
MluCI AATT 7 cut(s) 41, 88, 117, 170, 620, 666, 807
MlyI GAGTC 1 cut(s) 594
MmeI TCCRAC 3 cut(s) 37, 562, 781
MnlI CCTC 5 cut(s) 53, 217, 299, 368, 758
MseI TTAA 1 cut(s) 863
MslI CAYNNNNRTG 1 cut(s) 566
MspI CCGG 3 cut(s) 696, 714, 731
MspR9I CCNGG 2 cut(s) 697, 715
MunI CAATTG 2 cut(s) 117, 666
MwoI GCNNNNNNNGC 3 cut(s) 122, 330, 710
NciI CCSGG 2 cut(s) 697, 715
NdeII GATC 2 cut(s) 34, 244
NlaIII CATG 4 cut(s) 131, 225, 331, 464
NlaIV GGNNCC 5 cut(s) 82, 99, 124, 367, 511
NmuCI GTSAC 3 cut(s) 68, 301, 586
NspI RCATGY 1 cut(s) 464
OliI CACNNNNGTG 1 cut(s) 566
PfeI GAWTC 2 cut(s) 230, 727
PkrI GCNGC 5 cut(s) 115, 160, 323, 491, 751
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
PspEI GGTNACC 2 cut(s) 627, 691
PspFI CCCAGC 2 cut(s) 653, 704
PspN4I GGNNCC 5 cut(s) 82, 99, 124, 367, 511
PspOMI GGGCCC 1 cut(s) 509
PspPI GGNCC 5 cut(s) 98, 454, 509, 510, 803
RsaI GTAC 5 cut(s) 468, 476, 546, 710, 827
RsaNI GTAC 5 cut(s) 467, 475, 545, 709, 826
RseI CAYNNNNRTG 1 cut(s) 566
SaqAI TTAA 1 cut(s) 863
SatI GCNGC 5 cut(s) 114, 159, 322, 490, 750
Sau3AI GATC 2 cut(s) 34, 244
Sau96I GGNCC 5 cut(s) 98, 454, 509, 510, 803
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 1 cut(s) 594
ScrFI CCNGG 2 cut(s) 697, 715
SduI GDGCHC 4 cut(s) 85, 127, 335, 513
SinI GGWCC 2 cut(s) 454, 803
SmiMI CAYNNNNRTG 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 7 cut(s) 41, 88, 117, 170, 620, 666, 807
SsiI CCGC 3 cut(s) 5, 183, 321
SspMI CTAG 3 cut(s) 191, 377, 548
StyD4I CCNGG 2 cut(s) 695, 713
StyI CCWWGG 2 cut(s) 93, 499
TaaI ACNGT 5 cut(s) 307, 448, 592, 691, 756
TaiI ACGT 1 cut(s) 869
TasI AATT 7 cut(s) 41, 88, 117, 170, 620, 666, 807
TatI WGTACW 2 cut(s) 466, 474
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 2 cut(s) 230, 727
Tru1I TTAA 1 cut(s) 863
Tru9I TTAA 1 cut(s) 863
TscAI CASTG 2 cut(s) 597, 759
TseFI GTSAC 3 cut(s) 68, 301, 586
TseI GCWGC 4 cut(s) 113, 158, 489, 749
Tsp45I GTSAC 3 cut(s) 68, 301, 586
TspDTI ATGAA 4 cut(s) 144, 265, 455, 908
TspGWI ACGGA 2 cut(s) 259, 300
TspRI CASTG 2 cut(s) 597, 759
VpaK11BI GGWCC 2 cut(s) 454, 803
XapI RAATTY 1 cut(s) 88
XceI RCATGY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 2 cut(s) 397, 520
XspI CTAG 3 cut(s) 191, 377, 548
ZrmI AGTACT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.