RchiOBHm_Chr6g0245851

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014818
Physical Location & Seq
Forward (+)
1582740 .. 1583761
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22036

Sequence Viewer

Length: 765 bp
ATGGAGTCGATAATCGTCCCCCATGAAGCTGATCATAAATTGCCGAGAGCAGTTGGAAGTGGTACGGAAAATAGATCAGTGGAAACCAACTCCTTAAGGCAAAGATTTCTTGCTTCAATTGGTGCCCATGAATACTTTTCACCAGAGATGTGGAGAGCAGCTCTTACAGAGTTTGTAGCAACTGCGTGTCTAATGTTCACCTTGACGTCTTCAATCATCTCATGCTTGGATTCACATGAGGTTGATCCCAAGCTATTAATTAGTACCCTTTGCGGTGTTATAACTCTTGCTAGAGCTTCCATCTACATCTTGATGCAATGTGTTGGTTCAATTGTGGGATTTCTTATTATCAAGACTGTGATGGACCATAATACATCCCAAAAGTACTCCTTAGGTGGCTGCACAACACATGGAGTAGGCACCCAAACAGCATTGATGTTAGAATTTGTTTGCACATTTGTGGTTCTCTTTGTTGGAGTCACTGTGGCATTCGACAAGAAAAGGTGCAAGGAACTTGGTTTAGCAATGGTGTGTCTCGTGGTGGCCGGGGCTATGGCACTTGCAGTTTTTGTGTCCATTACAGTCACTGGGAAGTCCGGGAATGCAGGCGTGGGGCTGAACCCGGCAAGGTGCTTAGGACCTGCATTGTTGCAAGGGGGGATATTATGGGATGGTCATTGGGTTTTCTGGGTTGGCTCCATCTTGGCCTGCAGTGTGTACTATTGTTTCTCTTTGAACTTGCCCAAGGAGGGTTTGCCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko04962 ko04976 map04962 map04976
Pfam Domains
Protein Families
MIP

Protein Analysis

254

Amino Acids

27.25

Weight (kDa)

7.01

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 90 - 234 5.1e-23 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AatII GACGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 649
AccB1I GGYRCC 2 cut(s) 122, 419
AciI CCGC 1 cut(s) 273
AclWI GGATC 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 1 cut(s) 443
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 4 cut(s) 64, 265, 386, 719
AfiI CCNNNNNNNGG 1 cut(s) 749
AflII CTTAAG 1 cut(s) 94
AgsI TTSAA 4 cut(s) 117, 213, 330, 736
AleI CACNNNNGTG 1 cut(s) 458
AluBI AGCT 4 cut(s) 29, 161, 253, 296
AluI AGCT 4 cut(s) 29, 161, 253, 296
Alw26I GTCTC 1 cut(s) 539
AlwI GGATC 1 cut(s) 239
AlwNI CAGNNNCTG 1 cut(s) 587
AoxI GGCC 2 cut(s) 543, 705
ApeKI GCWGC 2 cut(s) 158, 399
ApoI RAATTY 1 cut(s) 443
AseI ATTAAT 1 cut(s) 257
AspS9I GGNCC 2 cut(s) 364, 638
AsuC2I CCSGG 3 cut(s) 547, 598, 623
AsuHPI GGTGA 2 cut(s) 132, 190
AvaII GGWCC 2 cut(s) 364, 638
AxyI CCTNAGG 1 cut(s) 391
BaeGI GKGCMC 1 cut(s) 127
BanI GGYRCC 2 cut(s) 122, 419
BauI CACGAG 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 170, 386
BccI CCATC 4 cut(s) 308, 355, 665, 707
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 3 cut(s) 547, 598, 623
BcoDI GTCTC 1 cut(s) 539
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 709
BfrI CTTAAG 1 cut(s) 94
BfuAI ACCTGC 1 cut(s) 649
BisI GCNGC 2 cut(s) 159, 400
BlsI GCNGC 2 cut(s) 160, 401
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 3 cut(s) 547, 598, 623
Bme18I GGWCC 2 cut(s) 364, 638
BmgT120I GGNCC 2 cut(s) 364, 638
BmiI GGNNCC 3 cut(s) 124, 421, 697
BmrFI CCNGG 3 cut(s) 547, 598, 623
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 1 cut(s) 303
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 1 cut(s) 201
BplI GAGNNNNNCTC 2 cut(s) 145, 177
Bpu10I CCTNAGC 1 cut(s) 634
BpuMI CCSGG 3 cut(s) 547, 598, 623
BsaHI GRCGYC 1 cut(s) 206
BsaJI CCNNGG 2 cut(s) 546, 744
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 749
Bse1I ACTGG 1 cut(s) 592
Bse21I CCTNAGG 1 cut(s) 391
Bse3DI GCAATG 2 cut(s) 323, 531
BseDI CCNNGG 2 cut(s) 546, 744
BseGI GGATG 2 cut(s) 374, 676
BseLI CCNNNNNNNGG 1 cut(s) 749
BseMI GCAATG 2 cut(s) 323, 531
BseNI ACTGG 1 cut(s) 592
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 2 cut(s) 170, 386
BsgI GTGCAG 1 cut(s) 385
BshFI GGCC 2 cut(s) 545, 707
BshNI GGYRCC 2 cut(s) 122, 419
BsiSI CCGG 3 cut(s) 546, 597, 623
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 1 cut(s) 749
BsmAI GTCTC 1 cut(s) 539
BsmFI GGGAC 1 cut(s) 2
BsmI GAATGC 2 cut(s) 488, 607
BsnI GGCC 2 cut(s) 545, 707
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 31, 74, 244
BspACI CCGC 1 cut(s) 273
BspANI GGCC 2 cut(s) 545, 707
BspLI GGNNCC 3 cut(s) 124, 421, 697
BspMAI CTGCAG 1 cut(s) 713
BspMI ACCTGC 1 cut(s) 649
BspPI GGATC 1 cut(s) 239
BspT107I GGYRCC 2 cut(s) 122, 419
BspTI CTTAAG 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 323, 531
BsrI ACTGG 1 cut(s) 592
BssECI CCNNGG 2 cut(s) 546, 744
BssMI GATC 3 cut(s) 31, 74, 244
BssNI GRCGYC 1 cut(s) 206
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 1 cut(s) 744
Bst2BI CACGAG 1 cut(s) 536
Bst4CI ACNGT 3 cut(s) 358, 484, 583
BstACI GRCGYC 1 cut(s) 206
BstAFI CTTAAG 1 cut(s) 94
BstC8I GCNNGC 2 cut(s) 607, 709
BstDEI CTNAG 2 cut(s) 391, 634
BstF5I GGATG 2 cut(s) 374, 676
BstKTI GATC 3 cut(s) 34, 77, 247
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 3 cut(s) 31, 74, 244
BstSCI CCNGG 3 cut(s) 545, 596, 621
BstSFI CTRYAG 1 cut(s) 709
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 2 cut(s) 170, 386
BstV2I GAAGAC 1 cut(s) 201
BstXI CCANNNNNNTGG 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 391
BsuRI GGCC 2 cut(s) 545, 707
BtsCI GGATG 2 cut(s) 374, 676
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 4 cut(s) 84, 480, 585, 718
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 2 cut(s) 607, 709
CaiI CAGNNNCTG 1 cut(s) 587
Cfr13I GGNCC 2 cut(s) 364, 638
Csp6I GTAC 4 cut(s) 63, 264, 385, 718
CviAII CATG 5 cut(s) 23, 128, 222, 236, 410
CviQI GTAC 4 cut(s) 63, 264, 385, 718
DdeI CTNAG 2 cut(s) 391, 634
DpnI GATC 3 cut(s) 33, 76, 246
DpnII GATC 3 cut(s) 31, 74, 244
EaeI YGGCCR 1 cut(s) 543
Eco130I CCWWGG 1 cut(s) 744
Eco47I GGWCC 2 cut(s) 364, 638
Eco81I CCTNAGG 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 744
ErhI CCWWGG 1 cut(s) 744
FaeI CATG 5 cut(s) 26, 131, 225, 239, 413
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 1 cut(s) 2
FatI CATG 5 cut(s) 22, 127, 221, 235, 409
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 2 cut(s) 159, 400
FokI GGATG 2 cut(s) 361, 683
Fsp4HI GCNGC 2 cut(s) 159, 400
FspBI CTAG 1 cut(s) 291
GluI GCNGC 2 cut(s) 159, 400
HaeIII GGCC 2 cut(s) 545, 707
HapII CCGG 3 cut(s) 546, 597, 623
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 5 cut(s) 26, 131, 225, 239, 413
HinfI GANTC 3 cut(s) 5, 230, 477
HpaII CCGG 3 cut(s) 546, 597, 623
HphI GGTGA 2 cut(s) 132, 190
Hpy166II GTNNAC 2 cut(s) 198, 718
Hpy188III TCNNGA 2 cut(s) 310, 352
Hpy8I GTNNAC 2 cut(s) 198, 718
HpyCH4III ACNGT 3 cut(s) 358, 484, 583
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 9 cut(s) 316, 402, 453, 507, 563, 605, 644, 652, 711
HpyF3I CTNAG 2 cut(s) 391, 634
HpySE526I ACGT 1 cut(s) 206
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 5 cut(s) 26, 131, 225, 239, 413
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 3 cut(s) 31, 74, 244
LmnI GCTCC 1 cut(s) 701
LpnPI CCDG 9 cut(s) 156, 559, 573, 591, 610, 636, 654, 673, 721
Lsp1109I GCAGC 2 cut(s) 170, 386
LweI GCATC 1 cut(s) 303
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 478, 583
MalI GATC 3 cut(s) 33, 76, 246
MboI GATC 3 cut(s) 31, 74, 244
MboII GAAGA 1 cut(s) 201
MfeI CAATTG 2 cut(s) 117, 330
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 5 cut(s) 38, 117, 258, 330, 443
MlyI GAGTC 2 cut(s) 14, 486
MmeI TCCRAC 2 cut(s) 34, 454
MnlI CCTC 2 cut(s) 232, 742
MseI TTAA 2 cut(s) 95, 257
MslI CAYNNNNRTG 2 cut(s) 311, 458
MspCI CTTAAG 1 cut(s) 94
MspI CCGG 3 cut(s) 546, 597, 623
MspR9I CCNGG 3 cut(s) 547, 598, 623
MunI CAATTG 2 cut(s) 117, 330
Mva1269I GAATGC 2 cut(s) 488, 607
NciI CCSGG 3 cut(s) 547, 598, 623
NdeII GATC 3 cut(s) 31, 74, 244
NlaIII CATG 5 cut(s) 26, 131, 225, 239, 413
NlaIV GGNNCC 3 cut(s) 124, 421, 697
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 478, 583
OliI CACNNNNGTG 1 cut(s) 458
PctI GAATGC 2 cut(s) 488, 607
PfeI GAWTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 596
PkrI GCNGC 2 cut(s) 160, 401
PleI GAGTC 2 cut(s) 13, 485
PpsI GAGTC 2 cut(s) 13, 485
PpuMI RGGWCCY 1 cut(s) 638
PshBI ATTAAT 1 cut(s) 257
PsiI TTATAA 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 638
PspN4I GGNNCC 3 cut(s) 124, 421, 697
PspPI GGNCC 2 cut(s) 364, 638
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 713
PstNI CAGNNNCTG 1 cut(s) 587
RsaI GTAC 4 cut(s) 64, 265, 386, 719
RsaNI GTAC 4 cut(s) 63, 264, 385, 718
RseI CAYNNNNRTG 2 cut(s) 311, 458
SaqAI TTAA 2 cut(s) 95, 257
SatI GCNGC 2 cut(s) 159, 400
Sau3AI GATC 3 cut(s) 31, 74, 244
Sau96I GGNCC 2 cut(s) 364, 638
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 2 cut(s) 14, 486
ScrFI CCNGG 3 cut(s) 547, 598, 623
SduI GDGCHC 1 cut(s) 127
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 1 cut(s) 709
SinI GGWCC 2 cut(s) 364, 638
SmiMI CAYNNNNRTG 2 cut(s) 311, 458
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 5 cut(s) 38, 117, 258, 330, 443
SsiI CCGC 1 cut(s) 273
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 545, 596, 621
StyI CCWWGG 1 cut(s) 744
TaaI ACNGT 3 cut(s) 358, 484, 583
TaiI ACGT 1 cut(s) 209
TaqI TCGA 2 cut(s) 8, 492
TasI AATT 5 cut(s) 38, 117, 258, 330, 443
TatI WGTACW 2 cut(s) 384, 717
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 2 cut(s) 95, 257
Tru9I TTAA 2 cut(s) 95, 257
TscAI CASTG 4 cut(s) 84, 487, 592, 718
TseFI GTSAC 2 cut(s) 478, 583
TseI GCWGC 2 cut(s) 158, 399
Tsp45I GTSAC 2 cut(s) 478, 583
TspDTI ATGAA 2 cut(s) 39, 144
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 4 cut(s) 84, 487, 592, 718
Vha464I CTTAAG 1 cut(s) 94
VpaK11BI GGWCC 2 cut(s) 364, 638
VspI ATTAAT 1 cut(s) 257
XapI RAATTY 1 cut(s) 443
XspI CTAG 1 cut(s) 291
ZraI GACGTC 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.