Rh6AG030900

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014818
Physical Location & Seq
Forward (+)
3820847 .. 3821861
1015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG030900.1

Sequence Viewer

Length: 855 bp
ATGGAGTCGATAATCGTCCCCCATGAAGCTGATCATAAATTGCCGAGAGCAGTTGGAAGTGGTACGGAAAATAGATCAGTGGAAACCAATTCCTTAAGGCAAAGATTTCTTGCTTCAATTGGTGCCCATGAATACTTTTCACCAGAGATGTGGAGAGCAGCTCTTACAGAGTTTGTAGCAACTGCGTGTCTAATGTTCACCTTGACGTCTTCAATCATCTCATGCTTGGATTCACATGAGGTTGATCCCAAGCTATTAGTACCCTTTGCGGTCTTCATCATAGCCTTCCTTTTCCTCATGGTGACAGTTCCTCTATCTGGTGGTCATATGAGTCCTGTTTTCACATTCATAGCTGCCCTAAAAGGTGTCATAACTCTTGCTAGAGCTTCCATCTACATCTTGATGCAATGTGTTGGTTCAATTGTGGGATTTCTTATTATCAAGACTGTGATGGACCATAATGCATCCCAAAAGTACTCCTTAGGTGGCTGCACAACACATGGAGTAGGCACCCAAACAGCATTGATGTTAGAATTTGTTTGCACATTTGTGGTTCTCTTTGTTGGAGTCACTGTAGCATTCGACAAGAAAAGGTGCAAGGAACTTGGTTTAGCAATGGTGTGTCTCGTGGTGGCCGGGGTTATGGCACTTGCAGTTTTTGTGTCCATTACAGTCACTGGGAAGTCCGGGTATGCAGGCGTGGGGCTGAACCCGGCAAGGTGCTTAGGACCTGCATTGTTGCAAGGAGGGATATTATGGGATGGTCATTGGGTTTTCTGGGTTGGCTCCATCTTGGCCTGCAGTGTGTACTATTGTTTCTCTTTGAACTTGCCCAAGGAGGGTTTGCCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko04962 ko04976 map04962 map04976
Pfam Domains
Protein Families
MIP

Protein Analysis

284

Amino Acids

30.55

Weight (kDa)

7.57

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 47 - 264 6.5e-33 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 739
AccB1I GGYRCC 2 cut(s) 122, 509
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 1 cut(s) 533
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 4 cut(s) 64, 261, 476, 809
AfiI CCNNNNNNNGG 2 cut(s) 317, 839
AflII CTTAAG 1 cut(s) 94
AgsI TTSAA 4 cut(s) 117, 213, 420, 826
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 5 cut(s) 29, 161, 253, 353, 386
AluI AGCT 5 cut(s) 29, 161, 253, 353, 386
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 1 cut(s) 239
AlwNI CAGNNNCTG 1 cut(s) 677
AoxI GGCC 2 cut(s) 633, 795
ApeKI GCWGC 3 cut(s) 158, 353, 489
ApoI RAATTY 1 cut(s) 533
AspS9I GGNCC 2 cut(s) 454, 728
AsuC2I CCSGG 3 cut(s) 637, 688, 713
AsuHPI GGTGA 3 cut(s) 132, 190, 313
AvaII GGWCC 2 cut(s) 454, 728
AxyI CCTNAGG 1 cut(s) 481
BaeGI GKGCMC 1 cut(s) 127
BanI GGYRCC 2 cut(s) 122, 509
BauI CACGAG 1 cut(s) 626
BbsI GAAGAC 2 cut(s) 201, 265
BbvI GCAGC 3 cut(s) 170, 340, 476
BccI CCATC 4 cut(s) 398, 445, 755, 797
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 3 cut(s) 637, 688, 713
BcoDI GTCTC 1 cut(s) 629
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 2 cut(s) 573, 799
BfrI CTTAAG 1 cut(s) 94
BfuAI ACCTGC 1 cut(s) 739
BisI GCNGC 3 cut(s) 159, 354, 490
BlsI GCNGC 3 cut(s) 160, 355, 491
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 3 cut(s) 637, 688, 713
Bme18I GGWCC 2 cut(s) 454, 728
BmgT120I GGNCC 2 cut(s) 454, 728
BmiI GGNNCC 3 cut(s) 124, 511, 787
BmrFI CCNGG 3 cut(s) 637, 688, 713
BmrI ACTGGG 1 cut(s) 687
BmsI GCATC 2 cut(s) 393, 473
BmuI ACTGGG 1 cut(s) 687
BpiI GAAGAC 2 cut(s) 201, 265
BplI GAGNNNNNCTC 2 cut(s) 145, 177
Bpu10I CCTNAGC 1 cut(s) 724
BpuMI CCSGG 3 cut(s) 637, 688, 713
BsaHI GRCGYC 1 cut(s) 206
BsaJI CCNNGG 2 cut(s) 636, 834
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 839
Bse1I ACTGG 1 cut(s) 682
Bse21I CCTNAGG 1 cut(s) 481
Bse3DI GCAATG 2 cut(s) 413, 621
BseDI CCNNGG 2 cut(s) 636, 834
BseGI GGATG 2 cut(s) 464, 766
BseLI CCNNNNNNNGG 2 cut(s) 317, 839
BseMI GCAATG 2 cut(s) 413, 621
BseNI ACTGG 1 cut(s) 682
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 3 cut(s) 170, 340, 476
BsgI GTGCAG 1 cut(s) 475
BshFI GGCC 2 cut(s) 635, 797
BshNI GGYRCC 2 cut(s) 122, 509
BsiSI CCGG 3 cut(s) 636, 687, 713
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 2 cut(s) 317, 839
BsmAI GTCTC 1 cut(s) 629
BsmFI GGGAC 1 cut(s) 2
BsmI GAATGC 1 cut(s) 578
BsnI GGCC 2 cut(s) 635, 797
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 31, 74, 244
BspACI CCGC 1 cut(s) 269
BspANI GGCC 2 cut(s) 635, 797
BspLI GGNNCC 3 cut(s) 124, 511, 787
BspMAI CTGCAG 1 cut(s) 803
BspMI ACCTGC 1 cut(s) 739
BspPI GGATC 1 cut(s) 239
BspT107I GGYRCC 2 cut(s) 122, 509
BspTI CTTAAG 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 413, 621
BsrI ACTGG 1 cut(s) 682
BssECI CCNNGG 2 cut(s) 636, 834
BssMI GATC 3 cut(s) 31, 74, 244
BssNI GRCGYC 1 cut(s) 206
BssSI CACGAG 1 cut(s) 626
BssT1I CCWWGG 1 cut(s) 834
Bst2BI CACGAG 1 cut(s) 626
Bst4CI ACNGT 4 cut(s) 307, 448, 574, 673
BstACI GRCGYC 1 cut(s) 206
BstAFI CTTAAG 1 cut(s) 94
BstC8I GCNNGC 2 cut(s) 697, 799
BstDEI CTNAG 2 cut(s) 481, 724
BstF5I GGATG 2 cut(s) 464, 766
BstKTI GATC 3 cut(s) 34, 77, 247
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 3 cut(s) 31, 74, 244
BstSCI CCNGG 3 cut(s) 635, 686, 711
BstSFI CTRYAG 2 cut(s) 573, 799
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 3 cut(s) 170, 340, 476
BstV2I GAAGAC 2 cut(s) 201, 265
BstXI CCANNNNNNTGG 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 481
BsuRI GGCC 2 cut(s) 635, 797
BtsCI GGATG 2 cut(s) 464, 766
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 4 cut(s) 84, 570, 675, 808
BveI ACCTGC 1 cut(s) 739
Cac8I GCNNGC 2 cut(s) 697, 799
CaiI CAGNNNCTG 1 cut(s) 677
Cfr13I GGNCC 2 cut(s) 454, 728
Csp6I GTAC 4 cut(s) 63, 260, 475, 808
CviAII CATG 6 cut(s) 23, 128, 222, 236, 298, 500
CviQI GTAC 4 cut(s) 63, 260, 475, 808
DdeI CTNAG 2 cut(s) 481, 724
DpnI GATC 3 cut(s) 33, 76, 246
DpnII GATC 3 cut(s) 31, 74, 244
EaeI YGGCCR 1 cut(s) 633
Eco130I CCWWGG 1 cut(s) 834
Eco47I GGWCC 2 cut(s) 454, 728
Eco81I CCTNAGG 1 cut(s) 481
EcoO109I RGGNCCY 1 cut(s) 728
EcoT14I CCWWGG 1 cut(s) 834
EcoT22I ATGCAT 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 834
FaeI CATG 6 cut(s) 26, 131, 225, 239, 301, 503
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 2
FatI CATG 6 cut(s) 22, 127, 221, 235, 297, 499
FauNDI CATATG 1 cut(s) 327
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 3 cut(s) 159, 354, 490
FokI GGATG 2 cut(s) 451, 773
Fsp4HI GCNGC 3 cut(s) 159, 354, 490
FspBI CTAG 1 cut(s) 381
GluI GCNGC 3 cut(s) 159, 354, 490
HaeIII GGCC 2 cut(s) 635, 797
HapII CCGG 3 cut(s) 636, 687, 713
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 6 cut(s) 26, 131, 225, 239, 301, 503
HinfI GANTC 4 cut(s) 5, 230, 331, 567
HpaII CCGG 3 cut(s) 636, 687, 713
HphI GGTGA 3 cut(s) 132, 190, 313
Hpy166II GTNNAC 2 cut(s) 198, 808
Hpy188III TCNNGA 2 cut(s) 400, 442
Hpy8I GTNNAC 2 cut(s) 198, 808
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 4 cut(s) 307, 448, 574, 673
HpyCH4IV ACGT 1 cut(s) 206
HpyF3I CTNAG 2 cut(s) 481, 724
HpySE526I ACGT 1 cut(s) 206
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 6 cut(s) 26, 131, 225, 239, 301, 503
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 3 cut(s) 31, 74, 244
LmnI GCTCC 1 cut(s) 791
Lsp1109I GCAGC 3 cut(s) 170, 340, 476
LweI GCATC 2 cut(s) 393, 473
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 3 cut(s) 301, 568, 673
MalI GATC 3 cut(s) 33, 76, 246
MboI GATC 3 cut(s) 31, 74, 244
MboII GAAGA 2 cut(s) 201, 265
MfeI CAATTG 2 cut(s) 117, 420
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 5 cut(s) 38, 88, 117, 420, 533
MlyI GAGTC 3 cut(s) 14, 340, 576
MmeI TCCRAC 2 cut(s) 34, 544
MnlI CCTC 5 cut(s) 232, 305, 321, 740, 832
Mph1103I ATGCAT 1 cut(s) 466
MseI TTAA 1 cut(s) 95
MslI CAYNNNNRTG 2 cut(s) 401, 548
MspCI CTTAAG 1 cut(s) 94
MspI CCGG 3 cut(s) 636, 687, 713
MspR9I CCNGG 3 cut(s) 637, 688, 713
MunI CAATTG 2 cut(s) 117, 420
Mva1269I GAATGC 1 cut(s) 578
NciI CCSGG 3 cut(s) 637, 688, 713
NdeI CATATG 1 cut(s) 327
NdeII GATC 3 cut(s) 31, 74, 244
NlaIII CATG 6 cut(s) 26, 131, 225, 239, 301, 503
NlaIV GGNNCC 3 cut(s) 124, 511, 787
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 3 cut(s) 301, 568, 673
NsiI ATGCAT 1 cut(s) 466
OliI CACNNNNGTG 1 cut(s) 548
PctI GAATGC 1 cut(s) 578
PfeI GAWTC 1 cut(s) 230
PkrI GCNGC 3 cut(s) 160, 355, 491
PleI GAGTC 3 cut(s) 13, 339, 575
PpsI GAGTC 3 cut(s) 13, 339, 575
PpuMI RGGWCCY 1 cut(s) 728
Psp5II RGGWCCY 1 cut(s) 728
PspN4I GGNNCC 3 cut(s) 124, 511, 787
PspPI GGNCC 2 cut(s) 454, 728
PspPPI RGGWCCY 1 cut(s) 728
PstI CTGCAG 1 cut(s) 803
PstNI CAGNNNCTG 1 cut(s) 677
RsaI GTAC 4 cut(s) 64, 261, 476, 809
RsaNI GTAC 4 cut(s) 63, 260, 475, 808
RseI CAYNNNNRTG 2 cut(s) 401, 548
SaqAI TTAA 1 cut(s) 95
SatI GCNGC 3 cut(s) 159, 354, 490
Sau3AI GATC 3 cut(s) 31, 74, 244
Sau96I GGNCC 2 cut(s) 454, 728
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 3 cut(s) 14, 340, 576
ScrFI CCNGG 3 cut(s) 637, 688, 713
SduI GDGCHC 1 cut(s) 127
SfaNI GCATC 2 cut(s) 393, 473
SfcI CTRYAG 2 cut(s) 573, 799
SinI GGWCC 2 cut(s) 454, 728
SmiMI CAYNNNNRTG 2 cut(s) 401, 548
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 5 cut(s) 38, 88, 117, 420, 533
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 3 cut(s) 635, 686, 711
StyI CCWWGG 1 cut(s) 834
TaaI ACNGT 4 cut(s) 307, 448, 574, 673
TaiI ACGT 1 cut(s) 209
TaqI TCGA 2 cut(s) 8, 582
TasI AATT 5 cut(s) 38, 88, 117, 420, 533
TatI WGTACW 2 cut(s) 474, 807
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 1 cut(s) 95
Tru9I TTAA 1 cut(s) 95
TscAI CASTG 4 cut(s) 84, 577, 682, 808
TseFI GTSAC 3 cut(s) 301, 568, 673
TseI GCWGC 3 cut(s) 158, 353, 489
Tsp45I GTSAC 3 cut(s) 301, 568, 673
TspDTI ATGAA 4 cut(s) 39, 144, 265, 337
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 4 cut(s) 84, 577, 682, 808
Vha464I CTTAAG 1 cut(s) 94
VpaK11BI GGWCC 2 cut(s) 454, 728
XapI RAATTY 1 cut(s) 533
XspI CTAG 1 cut(s) 381
ZraI GACGTC 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 476
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.