Rh6DG026600

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0014818
Physical Location & Seq
Forward (+)
2341287 .. 2341814
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG026600.1

Sequence Viewer

Length: 528 bp
ATGAGTCCTGTTTTCACATTCATAGCTGCCCTAAAAGGTGTCATAACTCTTGCTAGAGCTTCCATCTACATCTTGATGCAATGTGTTGGTTCAATTGTGGGATTTCTTATTATCAAGACTGTGATGGACCATAATGCATCCCAAAAGTACTCCTTAGGTGGCTGCACAACACATGGAGTAGGCACCCAAACAGCATTGATGTTAGAATTTGTTTGCACATTTGTGGTTCTCTTTGTTGGAGTCACTGTGGCGTTCGACAAGAAAAGGTGCAAGGAACTTGGTTTAGCAATGGTGTGTCTCGTGGTGGTCGGGGCTATGGCACTTGCAGTTTTTGTGTCCATTACAATCACTGGGAAGTCCGGGTATGCAGGCGTGGGGCTGAACCCGGCAAGGTGCTTAGGACCTGCATTGTTGCAAGGGGGAATATTATGGGATGGTCATTGGGTTTTCTGGGTTGGCTCCATCTTGGCCTGCAGTGTGTACTATTGTTTCTCTTTGAACTTGCCCAAGGAGGGTTTGCCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko04962 ko04976 map04962 map04976
Pfam Domains
Protein Families
MIP

Protein Analysis

175

Amino Acids

18.56

Weight (kDa)

8.82

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 3 - 155 6.7e-25 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 182
AcsI RAATTY 1 cut(s) 206
AfaI GTAC 2 cut(s) 149, 482
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 2 cut(s) 93, 499
AleI CACNNNNGTG 1 cut(s) 221
AluBI AGCT 2 cut(s) 26, 59
AluI AGCT 2 cut(s) 26, 59
Alw26I GTCTC 1 cut(s) 302
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 2 cut(s) 26, 162
ApoI RAATTY 1 cut(s) 206
AspS9I GGNCC 2 cut(s) 127, 401
AsuC2I CCSGG 2 cut(s) 361, 386
AvaII GGWCC 2 cut(s) 127, 401
AxyI CCTNAGG 1 cut(s) 154
BanI GGYRCC 1 cut(s) 182
BauI CACGAG 1 cut(s) 299
BbvI GCAGC 2 cut(s) 13, 149
BccI CCATC 4 cut(s) 71, 118, 428, 470
BcnI CCSGG 2 cut(s) 361, 386
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 412
BisI GCNGC 2 cut(s) 27, 163
BlsI GCNGC 2 cut(s) 28, 164
BmcAI AGTACT 1 cut(s) 149
Bme1390I CCNGG 2 cut(s) 361, 386
Bme18I GGWCC 2 cut(s) 127, 401
BmgT120I GGNCC 2 cut(s) 127, 401
BmiI GGNNCC 2 cut(s) 184, 460
BmrFI CCNGG 2 cut(s) 361, 386
BmrI ACTGGG 1 cut(s) 360
BmsI GCATC 2 cut(s) 66, 146
BmuI ACTGGG 1 cut(s) 360
Bpu10I CCTNAGC 1 cut(s) 397
BpuMI CCSGG 2 cut(s) 361, 386
BsaJI CCNNGG 1 cut(s) 507
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse1I ACTGG 1 cut(s) 355
Bse21I CCTNAGG 1 cut(s) 154
Bse3DI GCAATG 2 cut(s) 86, 294
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 2 cut(s) 137, 439
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMI GCAATG 2 cut(s) 86, 294
BseNI ACTGG 1 cut(s) 355
BseXI GCAGC 2 cut(s) 13, 149
BsgI GTGCAG 1 cut(s) 148
BshFI GGCC 1 cut(s) 470
BshNI GGYRCC 1 cut(s) 182
BsiSI CCGG 2 cut(s) 360, 386
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 1 cut(s) 302
BsnI GGCC 1 cut(s) 470
BspANI GGCC 1 cut(s) 470
BspLI GGNNCC 2 cut(s) 184, 460
BspMAI CTGCAG 1 cut(s) 476
BspMI ACCTGC 1 cut(s) 412
BspT107I GGYRCC 1 cut(s) 182
BsrDI GCAATG 2 cut(s) 86, 294
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 507
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 299
Bst4CI ACNGT 2 cut(s) 121, 247
BstC8I GCNNGC 2 cut(s) 370, 472
BstDEI CTNAG 2 cut(s) 154, 397
BstF5I GGATG 2 cut(s) 137, 439
BstMAI GTCTC 1 cut(s) 302
BstSCI CCNGG 2 cut(s) 359, 384
BstSFI CTRYAG 1 cut(s) 472
BstV1I GCAGC 2 cut(s) 13, 149
Bsu36I CCTNAGG 1 cut(s) 154
BsuRI GGCC 1 cut(s) 470
BtsCI GGATG 2 cut(s) 137, 439
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 3 cut(s) 243, 348, 481
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 370, 472
Cfr13I GGNCC 2 cut(s) 127, 401
Csp6I GTAC 2 cut(s) 148, 481
CviAII CATG 1 cut(s) 173
CviJI RGCY 7 cut(s) 26, 59, 162, 314, 379, 459, 470
CviKI_1 RGCY 7 cut(s) 26, 59, 162, 314, 379, 459, 470
CviQI GTAC 2 cut(s) 148, 481
DdeI CTNAG 2 cut(s) 154, 397
Eco130I CCWWGG 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 127, 401
Eco81I CCTNAGG 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 401
EcoT14I CCWWGG 1 cut(s) 507
EcoT22I ATGCAT 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 1 cut(s) 176
FaiI YATR 7 cut(s) 23, 44, 132, 174, 317, 366, 430
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 1 cut(s) 172
Fnu4HI GCNGC 2 cut(s) 27, 163
FokI GGATG 2 cut(s) 124, 446
Fsp4HI GCNGC 2 cut(s) 27, 163
FspBI CTAG 1 cut(s) 54
GluI GCNGC 2 cut(s) 27, 163
HaeIII GGCC 1 cut(s) 470
HapII CCGG 2 cut(s) 360, 386
Hin1II CATG 1 cut(s) 176
HinfI GANTC 2 cut(s) 4, 240
HpaII CCGG 2 cut(s) 360, 386
Hpy166II GTNNAC 1 cut(s) 481
Hpy188III TCNNGA 2 cut(s) 73, 115
Hpy8I GTNNAC 1 cut(s) 481
HpyCH4III ACNGT 2 cut(s) 121, 247
HpyF3I CTNAG 2 cut(s) 154, 397
Hsp92II CATG 1 cut(s) 176
LmnI GCTCC 1 cut(s) 464
LpnPI CCDG 8 cut(s) 21, 336, 354, 373, 399, 417, 436, 484
Lsp1109I GCAGC 2 cut(s) 13, 149
LweI GCATC 2 cut(s) 66, 146
MaeI CTAG 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 241
MfeI CAATTG 1 cut(s) 93
MluCI AATT 2 cut(s) 93, 206
MlyI GAGTC 2 cut(s) 13, 249
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 1 cut(s) 505
Mph1103I ATGCAT 1 cut(s) 139
MslI CAYNNNNRTG 2 cut(s) 74, 221
MspI CCGG 2 cut(s) 360, 386
MspR9I CCNGG 2 cut(s) 361, 386
MunI CAATTG 1 cut(s) 93
NciI CCSGG 2 cut(s) 361, 386
NlaIII CATG 1 cut(s) 176
NlaIV GGNNCC 2 cut(s) 184, 460
NmuCI GTSAC 1 cut(s) 241
NsiI ATGCAT 1 cut(s) 139
OliI CACNNNNGTG 1 cut(s) 221
PkrI GCNGC 2 cut(s) 28, 164
PleI GAGTC 2 cut(s) 12, 248
PpsI GAGTC 2 cut(s) 12, 248
PpuMI RGGWCCY 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 184, 460
PspPI GGNCC 2 cut(s) 127, 401
PspPPI RGGWCCY 1 cut(s) 401
PstI CTGCAG 1 cut(s) 476
RsaI GTAC 2 cut(s) 149, 482
RsaNI GTAC 2 cut(s) 148, 481
RseI CAYNNNNRTG 2 cut(s) 74, 221
SatI GCNGC 2 cut(s) 27, 163
Sau96I GGNCC 2 cut(s) 127, 401
ScaI AGTACT 1 cut(s) 149
SchI GAGTC 2 cut(s) 13, 249
ScrFI CCNGG 2 cut(s) 361, 386
SetI ASST 7 cut(s) 28, 40, 61, 160, 269, 395, 406
SfaNI GCATC 2 cut(s) 66, 146
SfcI CTRYAG 1 cut(s) 472
SinI GGWCC 2 cut(s) 127, 401
SmiMI CAYNNNNRTG 2 cut(s) 74, 221
Sse9I AATT 2 cut(s) 93, 206
SspI AATATT 1 cut(s) 426
SspMI CTAG 1 cut(s) 54
StyD4I CCNGG 2 cut(s) 359, 384
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 2 cut(s) 121, 247
TaqI TCGA 1 cut(s) 255
TasI AATT 2 cut(s) 93, 206
TatI WGTACW 2 cut(s) 147, 480
TscAI CASTG 3 cut(s) 250, 355, 481
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 2 cut(s) 26, 162
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 1 cut(s) 10
TspRI CASTG 3 cut(s) 250, 355, 481
VpaK11BI GGWCC 2 cut(s) 127, 401
XapI RAATTY 1 cut(s) 206
XspI CTAG 1 cut(s) 54
ZrmI AGTACT 1 cut(s) 149
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.