Rh6BG027700

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0014818
Physical Location & Seq
Forward (+)
4138479 .. 4139477
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG027700.1

Sequence Viewer

Length: 708 bp
ATGTGGAGAGCAGCTCTTACAGAGTTTGTAGCAACTGCGTGTCTAATGTTCACCTTGACGTCTTCAATCATCTCATGCTTGGATTCACATGAGGTTGATCCCAAGCTATTAGTACCCTTTGCGGTCTTCATCATAGCCTTCCTTTTCCTCATGGTGACAGTTCCTCTATCCGGTGGTCATATGAGTCCTGTTTTCACATTCATAGCTGCCCTAAAAGGTGTCATAACTCTTGCTAGAGCTTCCCTCTACATCTTGATGCAATGTGTTGGTTCAATTGTGGGATTTCTTATTATCAAGACTGTGATGGACCATAATGCATCCCAAAAGTACTCCTTAGGTGGCTGCACAACACATGGAGTAGGCACCCAAACAGCATTGATGTTAGAATTTGTTTGCACATTTGTGGTTCTCTTTGTTGGAGTCACTGTAGCATTTGACAAGAAAAGGTGCAAGGAACTTGGTTTAGCAATGGTGTGTCTCGTGGTGGCCGGGGCTATGGCACTTGCAGTTTTTGTGTCCATTACAGTCACTGGGAAGTCCGGGTATGCAGGAGTGGGGCTGAACCCGGCAAGGTGCTTAGGACCTGCATTGTTGCAAGGGGGGATATTATGGGACGGTCATTGGGTTTTCTGGGTTGGCTCCATCTTGGCCTGCAGTGTGTACTATTGTTTCTCTTTGAACTTGCCCAAGGAGGGTTTGCCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko04962 ko04976 map04962 map04976
Pfam Domains
Protein Families
MIP

Protein Analysis

235

Amino Acids

25.07

Weight (kDa)

8.33

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 2 - 215 5.1e-32 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 592
AccB1I GGYRCC 1 cut(s) 362
AciI CCGC 1 cut(s) 122
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 1 cut(s) 386
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 3 cut(s) 114, 329, 662
AfiI CCNNNNNNNGG 2 cut(s) 170, 692
AgsI TTSAA 3 cut(s) 66, 273, 679
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 4 cut(s) 14, 106, 206, 239
AluI AGCT 4 cut(s) 14, 106, 206, 239
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 1 cut(s) 92
AlwNI CAGNNNCTG 1 cut(s) 530
AoxI GGCC 2 cut(s) 486, 648
ApeKI GCWGC 3 cut(s) 11, 206, 342
ApoI RAATTY 1 cut(s) 386
AspS9I GGNCC 2 cut(s) 307, 581
AsuC2I CCSGG 3 cut(s) 490, 541, 566
AsuHPI GGTGA 2 cut(s) 43, 166
AvaII GGWCC 2 cut(s) 307, 581
AxyI CCTNAGG 1 cut(s) 334
BanI GGYRCC 1 cut(s) 362
BauI CACGAG 1 cut(s) 479
BbsI GAAGAC 2 cut(s) 54, 118
BbvI GCAGC 3 cut(s) 23, 193, 329
BccI CCATC 2 cut(s) 298, 650
BcnI CCSGG 3 cut(s) 490, 541, 566
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 2 cut(s) 426, 652
BfuAI ACCTGC 1 cut(s) 592
BisI GCNGC 3 cut(s) 12, 207, 343
BlsI GCNGC 3 cut(s) 13, 208, 344
BmcAI AGTACT 1 cut(s) 329
Bme1390I CCNGG 3 cut(s) 490, 541, 566
Bme18I GGWCC 2 cut(s) 307, 581
BmgT120I GGNCC 2 cut(s) 307, 581
BmiI GGNNCC 2 cut(s) 364, 640
BmrFI CCNGG 3 cut(s) 490, 541, 566
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 2 cut(s) 246, 326
BmuI ACTGGG 1 cut(s) 540
BpiI GAAGAC 2 cut(s) 54, 118
BplI GAGNNNNNCTC 3 cut(s) 30, 228, 260
Bpu10I CCTNAGC 1 cut(s) 577
BpuMI CCSGG 3 cut(s) 490, 541, 566
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 489, 687
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 692
Bse1I ACTGG 1 cut(s) 535
Bse21I CCTNAGG 1 cut(s) 334
Bse3DI GCAATG 2 cut(s) 266, 474
BseDI CCNNGG 2 cut(s) 489, 687
BseGI GGATG 1 cut(s) 317
BseLI CCNNNNNNNGG 2 cut(s) 170, 692
BseMI GCAATG 2 cut(s) 266, 474
BseNI ACTGG 1 cut(s) 535
BseXI GCAGC 3 cut(s) 23, 193, 329
BsgI GTGCAG 1 cut(s) 328
BshFI GGCC 2 cut(s) 488, 650
BshNI GGYRCC 1 cut(s) 362
BsiSI CCGG 4 cut(s) 171, 489, 540, 566
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 2 cut(s) 170, 692
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 2 cut(s) 488, 650
Bsp143I GATC 1 cut(s) 97
BspACI CCGC 1 cut(s) 122
BspANI GGCC 2 cut(s) 488, 650
BspLI GGNNCC 2 cut(s) 364, 640
BspMAI CTGCAG 1 cut(s) 656
BspMI ACCTGC 1 cut(s) 592
BspPI GGATC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 362
BsrDI GCAATG 2 cut(s) 266, 474
BsrI ACTGG 1 cut(s) 535
BssECI CCNNGG 2 cut(s) 489, 687
BssMI GATC 1 cut(s) 97
BssNI GRCGYC 1 cut(s) 59
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 687
Bst2BI CACGAG 1 cut(s) 479
Bst4CI ACNGT 5 cut(s) 160, 301, 427, 526, 617
BstACI GRCGYC 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 652
BstDEI CTNAG 2 cut(s) 334, 577
BstF5I GGATG 1 cut(s) 317
BstKTI GATC 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 1 cut(s) 97
BstSCI CCNGG 3 cut(s) 488, 539, 564
BstSFI CTRYAG 2 cut(s) 426, 652
BstV1I GCAGC 3 cut(s) 23, 193, 329
BstV2I GAAGAC 2 cut(s) 54, 118
Bsu36I CCTNAGG 1 cut(s) 334
BsuRI GGCC 2 cut(s) 488, 650
BtsCI GGATG 1 cut(s) 317
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 3 cut(s) 423, 528, 661
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 1 cut(s) 652
CaiI CAGNNNCTG 1 cut(s) 530
Cfr13I GGNCC 2 cut(s) 307, 581
Csp6I GTAC 3 cut(s) 113, 328, 661
CviAII CATG 4 cut(s) 75, 89, 151, 353
CviQI GTAC 3 cut(s) 113, 328, 661
DdeI CTNAG 2 cut(s) 334, 577
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
EaeI YGGCCR 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 687
Eco47I GGWCC 2 cut(s) 307, 581
Eco81I CCTNAGG 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 581
EcoT14I CCWWGG 1 cut(s) 687
EcoT22I ATGCAT 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 687
FaeI CATG 4 cut(s) 78, 92, 154, 356
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 1 cut(s) 626
FatI CATG 4 cut(s) 74, 88, 150, 352
FauNDI CATATG 1 cut(s) 180
Fnu4HI GCNGC 3 cut(s) 12, 207, 343
FokI GGATG 1 cut(s) 304
Fsp4HI GCNGC 3 cut(s) 12, 207, 343
FspBI CTAG 1 cut(s) 234
GluI GCNGC 3 cut(s) 12, 207, 343
HaeIII GGCC 2 cut(s) 488, 650
HapII CCGG 4 cut(s) 171, 489, 540, 566
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 4 cut(s) 78, 92, 154, 356
HinfI GANTC 3 cut(s) 83, 184, 420
HpaII CCGG 4 cut(s) 171, 489, 540, 566
HphI GGTGA 2 cut(s) 43, 166
Hpy166II GTNNAC 2 cut(s) 51, 661
Hpy188III TCNNGA 2 cut(s) 253, 295
Hpy8I GTNNAC 2 cut(s) 51, 661
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 5 cut(s) 160, 301, 427, 526, 617
HpyCH4IV ACGT 1 cut(s) 59
HpyF3I CTNAG 2 cut(s) 334, 577
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 4 cut(s) 78, 92, 154, 356
Kzo9I GATC 1 cut(s) 97
LmnI GCTCC 1 cut(s) 644
Lsp1109I GCAGC 3 cut(s) 23, 193, 329
LweI GCATC 2 cut(s) 246, 326
MaeI CTAG 1 cut(s) 234
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 3 cut(s) 154, 421, 526
MalI GATC 1 cut(s) 99
MboI GATC 1 cut(s) 97
MboII GAAGA 2 cut(s) 54, 118
MfeI CAATTG 1 cut(s) 273
MluCI AATT 2 cut(s) 273, 386
MlyI GAGTC 2 cut(s) 193, 429
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 5 cut(s) 85, 158, 174, 254, 685
Mph1103I ATGCAT 1 cut(s) 319
MslI CAYNNNNRTG 2 cut(s) 254, 401
MspI CCGG 4 cut(s) 171, 489, 540, 566
MspR9I CCNGG 3 cut(s) 490, 541, 566
MunI CAATTG 1 cut(s) 273
NciI CCSGG 3 cut(s) 490, 541, 566
NdeI CATATG 1 cut(s) 180
NdeII GATC 1 cut(s) 97
NlaIII CATG 4 cut(s) 78, 92, 154, 356
NlaIV GGNNCC 2 cut(s) 364, 640
NmuCI GTSAC 3 cut(s) 154, 421, 526
NsiI ATGCAT 1 cut(s) 319
OliI CACNNNNGTG 1 cut(s) 401
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 3 cut(s) 13, 208, 344
PleI GAGTC 2 cut(s) 192, 428
PpsI GAGTC 2 cut(s) 192, 428
PpuMI RGGWCCY 1 cut(s) 581
Psp5II RGGWCCY 1 cut(s) 581
PspN4I GGNNCC 2 cut(s) 364, 640
PspPI GGNCC 2 cut(s) 307, 581
PspPPI RGGWCCY 1 cut(s) 581
PstI CTGCAG 1 cut(s) 656
PstNI CAGNNNCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 114, 329, 662
RsaNI GTAC 3 cut(s) 113, 328, 661
RseI CAYNNNNRTG 2 cut(s) 254, 401
SatI GCNGC 3 cut(s) 12, 207, 343
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 2 cut(s) 307, 581
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 2 cut(s) 193, 429
ScrFI CCNGG 3 cut(s) 490, 541, 566
SfaNI GCATC 2 cut(s) 246, 326
SfcI CTRYAG 2 cut(s) 426, 652
SinI GGWCC 2 cut(s) 307, 581
SmiMI CAYNNNNRTG 2 cut(s) 254, 401
Sse9I AATT 2 cut(s) 273, 386
SsiI CCGC 1 cut(s) 122
SspMI CTAG 1 cut(s) 234
StyD4I CCNGG 3 cut(s) 488, 539, 564
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 5 cut(s) 160, 301, 427, 526, 617
TaiI ACGT 1 cut(s) 62
TasI AATT 2 cut(s) 273, 386
TatI WGTACW 2 cut(s) 327, 660
TfiI GAWTC 1 cut(s) 83
TscAI CASTG 3 cut(s) 430, 535, 661
TseFI GTSAC 3 cut(s) 154, 421, 526
TseI GCWGC 3 cut(s) 11, 206, 342
Tsp45I GTSAC 3 cut(s) 154, 421, 526
TspDTI ATGAA 2 cut(s) 118, 190
TspRI CASTG 3 cut(s) 430, 535, 661
VpaK11BI GGWCC 2 cut(s) 307, 581
XapI RAATTY 1 cut(s) 386
XspI CTAG 1 cut(s) 234
ZraI GACGTC 1 cut(s) 60
ZrmI AGTACT 1 cut(s) 329
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.