Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004730

Physical Location & Seq

Genomic Coordinates Reverse (-)

25235138 .. 25238308

Gene Structure 3171 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1128400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGGGGAGTGTGTCGTCGGAGGACGGCTCGGACCAGCAGAGCGAGCGGTGCGGGAGCTACAGCCTGAGCGCCGATGTGAGCGAGTCCGAGAGTTGCAGCAGCTTCTCGTGCAGGAGGTTCGACGCGGAAGGGGCTTCGAGCTCCATGACGTCGTCTCCGTGCCCTGTTGCCGGAAATTTCTGTTTTCAGCCGCCGGTGATGCTTCCGGTGATCGGAGGGAAGGATGTGGTTGCTTGGGATGAGAATCCGGAGAAGCGAGATGCTGATTTATCTGAGGTTGAAATGATGAAGGAGAGGTTCGCAAAGCTTTTGCTTGGAGAAGACATGTCCGGAGGTGGGAAAGGAGTCTGCACTGCCCTTGCTATCTCAAATGCCATCACCAATCTCTCTGCAACCGTGTTTGGCGAATTATGGAGGTTAGAGCCGCTGAAAGCGCAGAGAAAGGCAATGTGGCGCCGGGAAATGGAGTGGCTCTTATGTGTGAGTGATTCAATTGTTGAGCTTGTGCCTTCAATACAACAGTTTCCAGGTGGGGGTACATATGAAGTCATGGAGATGCGGCCGCGCTCTGACTTGTATGTGAATCTACCTGCCATAAAGAAGCTTGATGCAATGCTGCTTAGTATGCTGGATGGATTTTGTGAGACGGAGTTCTGCTATATTGACCGGGCGATAATTTTGGGCGATTCGAAAGAGGGTGAGGGGTTTATGGGTGGAAGGCCTTCGATTAGGCAGGAAGAGAAGTGGTGGTTGCCCTATCCTAAAGTTCCGCCAAATGGGTTGTCCTGTGAAACAAGGAAGAAGTTGCAGCAGTGTAGGGACTGCTGCAACCAGATATTGAAGGCGGCCATGGCGATTAATAGTAGTGTGCTTGTTGAGATGGAAATCCCGAGAGCATACATGGAAACGTTGCCCAAGAATGGCAAGGATTGTCTGGGCGATATCATTTATCGTTACATAACTGCAGACCAGTTTTCACCCGAACATCTTCTTGATTGCCTGGACTTGTCGTCAGAACATCAAACTCTAGAGATAGCCAATAGAATTGAGGCAGCTGTGCATGTTTGGAAGCAAAAAGACCAGAAGAAACACAACAATCATAAAGCTAAACGGGCATCTTGGGGGGGCAAGGTCAAGGGCCTTGTTGCTGATACTGAAAAGAACCATTTCCTGGCCCAACGAGCAGAGACCCTCTTACACAGCCTAAGGCATCGTTTCCCTGGCCTCCCGCAAACTTCACTAGATATGAACAAAATACAGTATAACAAGGATGTGGGGCAGTCAATTCTGGAAAGCTACTCAAGAGTTATGGAGAGTCTGGCCTTTAACATAATGGCAAGAATAGATGATGTCCTCTTCGTTGACGATGCTACCAAGCGATGTGCTGCAGCGGAATCAGTGTCTATCTTCAGCAGGGGAGGTTTGAGTGGCCTTCCTATCCAGAAGCGGATGTCGCCAAGCCCCTTCTCCATTCAACACACTCCTTACGCGTCTCCACTTGCAACACCGACTTTCTGTTCCTCCACCCCAGTTATTGGAAGTCCAGGAAGGACACCTCTGTGTGTAAAGCGCAGTGTTATATTAGACGAGAAGTCAGAGAAACTACTCGCGGCTGATTTTGAGAGGGTGTGGTCGTACGCCGGAAGCCTAAGCTCAAGGAGAACTACCGGCGACGCTCCTGAACGGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010769 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0031267 GO:0032092 GO:0032501 GO:0032502 GO:0032879 GO:0032989 GO:0040007 GO:0040008 GO:0043393 GO:0043900 GO:0044093 GO:0044425 GO:0044459 GO:0044464 GO:0044706 GO:0045177 GO:0045595 GO:0048468 GO:0048518 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051020 GO:0051049 GO:0051098 GO:0051099 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0065008 GO:0065009 GO:0071840 GO:0071944 GO:0080092 GO:0098590 GO:0098772 GO:1904424 GO:1904426 GO:1904475 GO:1904477 GO:1905097 GO:1905099 GO:2000012 GO:2000241 GO:2001106 GO:2001108

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PRONE

Protein Analysis

564

Amino Acids

62.47

Weight (kDa)

5.87

Isoelectric Point (pI)

53.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRONE	PF03759	91 - 459	1.2e-179	PRONE (Plant-specific Rop nucleotide exchanger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004730)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38430
fragaria_vesca	FvH4_2g15940 FvH4_2g37830 FvH4_2g37830
malus_domestica	MD05G1128400.v1.1 MD08G1005700.v1.1 MD10G1131700.v1.1
prunus_persica	Prupe.1G357600_v2.0.a1 Prupe.8G172500_v2.0.a1
pyrus_communis	pycom05g12430 pycom08g00440 pycom10g11270
rosa_chinensis	RchiOBHm_Chr6g0279461 RchiOBHm_Chr6g0303881
rosa_laevigata	RLG00000011038 RLG00000013145
rosa_multiflora	Rmu_sc0000274.1_g000004 Rmu_sc0004644.1_g000003 Rmu_sc0007179.1_g000004
rosa_roxburghii	Rroxscaffold_7G00164340 Rroxscaffold_7G00188890
rosa_rugosa	Rorug06G0124300 Rorug06G0325500 Rorug06G0325500
rosa_samantha	Rh6AG439800 Rh6BG238000 Rh6BG463200 Rh6CG452100 Rh6DG439000
rosa_wichuraiana	Rw6G020390 Rw6G038080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	152
Acc36I	ACCTGC	1 cut(s)	598
AccB1I	GGYRCC	1 cut(s)	453
AccB7I	CCANNNNNTGG	2 cut(s)	1171, 1535
AccBSI	CCGCTC	1 cut(s)	46
AccII	CGCG	4 cut(s)	125, 565, 1490, 1610
AccIII	TCCGGA	2 cut(s)	247, 329
AclI	AACGTT	1 cut(s)	908
AcoI	YGGCCR	2 cut(s)	560, 846
AcsI	RAATTY	1 cut(s)	175
AcuI	CTGAAG	1 cut(s)	1393
AcyI	GRCGYC	2 cut(s)	149, 454
AfaI	GTAC	2 cut(s)	538, 1637
AfiI	CCNNNNNNNGG	9 cut(s)	170, 212, 336, 463, 533, 776, 920, 1171, 1535
AflIII	ACRYGT	2 cut(s)	324, 1488
AgsI	TTSAA	5 cut(s)	281, 492, 513, 841, 1475
AhdI	GACNNNNNGTC	2 cut(s)	1009, 1591
AjnI	CCWGG	5 cut(s)	526, 999, 1170, 1219, 1543
AleI	CACNNNNGTG	1 cut(s)	1558
Alw21I	GWGCWC	1 cut(s)	143
Alw26I	GTCTC	4 cut(s)	159, 638, 1181, 1497
Ama87I	CYCGRG	1 cut(s)	889
Aor13HI	TCCGGA	2 cut(s)	247, 329
AoxI	GGCC	8 cut(s)	560, 719, 846, 1138, 1173, 1222, 1320, 1429
ApeKI	GCWGC	8 cut(s)	96, 99, 616, 808, 825, 1052, 1385, 1388
ApoI	RAATTY	1 cut(s)	175
ArsI	GACNNNNNNTTYG	2 cut(s)	767, 799
AseI	ATTAAT	1 cut(s)	858
Asp700I	GAANNNNTTC	3 cut(s)	721, 987, 1166
AspLEI	GCGC	5 cut(s)	71, 436, 456, 567, 1572
AspS9I	GGNCC	3 cut(s)	31, 1138, 1174
AsuC2I	CCSGG	2 cut(s)	458, 668
AsuHPI	GGTGA	5 cut(s)	208, 220, 370, 710, 969
AsuII	TTCGAA	1 cut(s)	689
AvaI	CYCGRG	1 cut(s)	889
AvaII	GGWCC	1 cut(s)	31
AxyI	CCTNAGG	1 cut(s)	1205
BaeGI	GKGCMC	1 cut(s)	164
BanI	GGYRCC	1 cut(s)	453
BanII	GRGCYC	1 cut(s)	143
BauI	CACGAG	1 cut(s)	106
BbsI	GAAGAC	1 cut(s)	327
Bbv12I	GWGCWC	1 cut(s)	143
BbvI	GCAGC	8 cut(s)	108, 111, 603, 812, 820, 1064, 1372, 1400
BccI	CCATC	3 cut(s)	383, 626, 874
BceAI	ACGGC	1 cut(s)	40
BcgI	CGANNNNNNTGC	2 cut(s)	100, 134
BciT130I	CCWGG	5 cut(s)	528, 1001, 1172, 1221, 1545
BcnI	CCSGG	2 cut(s)	458, 668
BcoDI	GTCTC	4 cut(s)	159, 638, 1181, 1497
BfaI	CTAG	2 cut(s)	1028, 1241
BfmI	CTRYAG	3 cut(s)	58, 963, 1386
BfoI	RGCGCY	2 cut(s)	72, 457
BfuAI	ACCTGC	1 cut(s)	598
Bme1390I	CCNGG	7 cut(s)	458, 528, 668, 1001, 1172, 1221, 1545
Bme18I	GGWCC	1 cut(s)	31
BmeRI	GACNNNNNGTC	2 cut(s)	1009, 1591
BmeT110I	CYCGRG	1 cut(s)	889
BmgT120I	GGNCC	3 cut(s)	31, 1138, 1174
BmiI	GGNNCC	1 cut(s)	455
BmrFI	CCNGG	7 cut(s)	458, 528, 668, 1001, 1172, 1221, 1545
BmrI	ACTGGG	1 cut(s)	1523
BmsI	GCATC	7 cut(s)	189, 250, 546, 598, 1124, 1219, 1357
BmuI	ACTGGG	1 cut(s)	1523
BpiI	GAAGAC	1 cut(s)	327
BplI	GAGNNNNNCTC	2 cut(s)	11, 43
Bpu10I	CCTNAGC	2 cut(s)	65, 1649
Bpu14I	TTCGAA	1 cut(s)	689
BpuEI	CTTGAG	2 cut(s)	1285, 1639
BpuMI	CCSGG	2 cut(s)	458, 668
BsaBI	GATNNNNATC	2 cut(s)	243, 884
BsaHI	GRCGYC	2 cut(s)	149, 454
BsaI	GGTCTC	1 cut(s)	1181
BsaJI	CCNNGG	2 cut(s)	849, 1219
BsaWI	WCCGGW	3 cut(s)	205, 247, 329
BsaXI	ACNNNNNCTCC	2 cut(s)	139, 169
Bsc4I	CCNNNNNNNGG	9 cut(s)	170, 212, 336, 463, 533, 776, 920, 1171, 1535
Bse118I	RCCGGY	2 cut(s)	193, 1667
Bse1I	ACTGG	2 cut(s)	970, 1529
Bse21I	CCTNAGG	1 cut(s)	1205
Bse3DI	GCAATG	2 cut(s)	453, 618
Bse8I	GATNNNNATC	2 cut(s)	243, 884
BseAI	TCCGGA	2 cut(s)	247, 329
BseBI	CCWGG	5 cut(s)	528, 1001, 1172, 1221, 1545
BseDI	CCNNGG	2 cut(s)	849, 1219
BseGI	GGATG	5 cut(s)	229, 244, 637, 1276, 1455
BseJI	GATNNNNATC	2 cut(s)	243, 884
BseLI	CCNNNNNNNGG	9 cut(s)	170, 212, 336, 463, 533, 776, 920, 1171, 1535
BseMI	GCAATG	2 cut(s)	453, 618
BseMII	CTCAG	2 cut(s)	56, 264
BseNI	ACTGG	2 cut(s)	970, 1529
BseSI	GKGCMC	1 cut(s)	164
BseX3I	CGGCCG	1 cut(s)	560
BseXI	GCAGC	8 cut(s)	108, 111, 603, 812, 820, 1064, 1372, 1400
BsgI	GTGCAG	2 cut(s)	130, 334
Bsh1236I	CGCG	4 cut(s)	125, 565, 1490, 1610
Bsh1285I	CGRYCG	1 cut(s)	563
BshFI	GGCC	8 cut(s)	562, 721, 848, 1140, 1175, 1224, 1322, 1431
BshNI	GGYRCC	1 cut(s)	453
BsiEI	CGRYCG	1 cut(s)	563
BsiHKAI	GWGCWC	1 cut(s)	143
BsiHKCI	CYCGRG	1 cut(s)	889
BsiSI	CCGG	9 cut(s)	171, 194, 206, 248, 330, 457, 667, 1641, 1668
BsiWI	CGTACG	1 cut(s)	1635
BslFI	GGGAC	1 cut(s)	833
BslI	CCNNNNNNNGG	9 cut(s)	170, 212, 336, 463, 533, 776, 920, 1171, 1535
BsmAI	GTCTC	4 cut(s)	159, 638, 1181, 1497
BsmBI	CGTCTC	3 cut(s)	159, 638, 1497
BsmFI	GGGAC	1 cut(s)	833
BsnI	GGCC	8 cut(s)	562, 721, 848, 1140, 1175, 1224, 1322, 1431
Bso31I	GGTCTC	1 cut(s)	1181
BsoBI	CYCGRG	1 cut(s)	889
Bsp119I	TTCGAA	1 cut(s)	689
Bsp1286I	GDGCHC	2 cut(s)	143, 164
Bsp13I	TCCGGA	2 cut(s)	247, 329
Bsp143I	GATC	1 cut(s)	210
Bsp19I	CCATGG	1 cut(s)	849
BspANI	GGCC	8 cut(s)	562, 721, 848, 1140, 1175, 1224, 1322, 1431
BspCNI	CTCAG	2 cut(s)	57, 265
BspEI	TCCGGA	2 cut(s)	247, 329
BspFNI	CGCG	4 cut(s)	125, 565, 1490, 1610
BspLI	GGNNCC	1 cut(s)	455
BspMAI	CTGCAG	2 cut(s)	967, 1390
BspMI	ACCTGC	1 cut(s)	598
BspT104I	TTCGAA	1 cut(s)	689
BspT107I	GGYRCC	1 cut(s)	453
BspTNI	GGTCTC	1 cut(s)	1181
BsrBI	CCGCTC	1 cut(s)	46
BsrDI	GCAATG	2 cut(s)	453, 618
BsrFI	RCCGGY	2 cut(s)	193, 1667
BsrI	ACTGG	2 cut(s)	970, 1529
BssAI	RCCGGY	2 cut(s)	193, 1667
BssECI	CCNNGG	2 cut(s)	849, 1219
BssMI	GATC	1 cut(s)	210
BssNI	GRCGYC	2 cut(s)	149, 454
BssSI	CACGAG	1 cut(s)	106
BssT1I	CCWWGG	1 cut(s)	849
Bst2BI	CACGAG	1 cut(s)	106
Bst2UI	CCWGG	5 cut(s)	528, 1001, 1172, 1221, 1545
Bst4CI	ACNGT	3 cut(s)	397, 522, 1260
Bst6I	CTCTTC	2 cut(s)	732, 1361
BstACI	GRCGYC	2 cut(s)	149, 454
BstBI	TTCGAA	1 cut(s)	689
BstC8I	GCNNGC	1 cut(s)	44
BstDEI	CTNAG	5 cut(s)	65, 273, 620, 1205, 1649
BstDSI	CCRYGG	1 cut(s)	849
BstF5I	GGATG	5 cut(s)	229, 244, 637, 1276, 1455
BstFNI	CGCG	4 cut(s)	125, 565, 1490, 1610
BstH2I	RGCGCY	2 cut(s)	72, 457
BstHHI	GCGC	5 cut(s)	71, 436, 456, 567, 1572
BstKTI	GATC	1 cut(s)	213
BstMAI	GTCTC	4 cut(s)	159, 638, 1181, 1497
BstMBI	GATC	1 cut(s)	210
BstMCI	CGRYCG	1 cut(s)	563
BstNI	CCWGG	5 cut(s)	528, 1001, 1172, 1221, 1545
BstNSI	RCATGY	2 cut(s)	328, 1064
BstSCI	CCNGG	7 cut(s)	456, 526, 666, 999, 1170, 1219, 1543
BstSFI	CTRYAG	3 cut(s)	58, 963, 1386
BstSLI	GKGCMC	1 cut(s)	164
BstUI	CGCG	4 cut(s)	125, 565, 1490, 1610
BstV1I	GCAGC	8 cut(s)	108, 111, 603, 812, 820, 1064, 1372, 1400
BstV2I	GAAGAC	1 cut(s)	327
BstZI	CGGCCG	1 cut(s)	560
Bsu36I	CCTNAGG	1 cut(s)	1205
BsuRI	GGCC	8 cut(s)	562, 721, 848, 1140, 1175, 1224, 1322, 1431
BtgI	CCRYGG	1 cut(s)	849
BtgZI	GCGATG	1 cut(s)	1393
BtsCI	GGATG	5 cut(s)	229, 244, 637, 1276, 1455
BtsI	GCAGTG	3 cut(s)	351, 818, 1579
BtsIMutI	CAGTG	4 cut(s)	351, 818, 1404, 1579
BveI	ACCTGC	1 cut(s)	598
Cac8I	GCNNGC	1 cut(s)	44
CciNI	GCGGCCGC	1 cut(s)	560
CfoI	GCGC	5 cut(s)	71, 436, 456, 567, 1572
Cfr10I	RCCGGY	2 cut(s)	193, 1667
Cfr13I	GGNCC	3 cut(s)	31, 1138, 1174
CseI	GACGC	3 cut(s)	131, 1479, 1682
Csp6I	GTAC	2 cut(s)	537, 1636
CviAII	CATG	6 cut(s)	145, 325, 550, 850, 901, 1061
CviQI	GTAC	2 cut(s)	537, 1636
DdeI	CTNAG	5 cut(s)	65, 273, 620, 1205, 1649
DinI	GGCGCC	1 cut(s)	455
DpnI	GATC	1 cut(s)	212
DpnII	GATC	1 cut(s)	210
DriI	GACNNNNNGTC	2 cut(s)	1009, 1591
EaeI	YGGCCR	2 cut(s)	560, 846
EagI	CGGCCG	1 cut(s)	560
Eam1104I	CTCTTC	2 cut(s)	732, 1361
Eam1105I	GACNNNNNGTC	2 cut(s)	1009, 1591
EarI	CTCTTC	2 cut(s)	732, 1361
EciI	GGCGGA	1 cut(s)	759
Ecl136II	GAGCTC	1 cut(s)	141
EclXI	CGGCCG	1 cut(s)	560
Eco130I	CCWWGG	1 cut(s)	849
Eco147I	AGGCCT	1 cut(s)	721
Eco24I	GRGCYC	1 cut(s)	143
Eco31I	GGTCTC	1 cut(s)	1181
Eco32I	GATATC	1 cut(s)	943
Eco47I	GGWCC	1 cut(s)	31
Eco52I	CGGCCG	1 cut(s)	560
Eco53kI	GAGCTC	1 cut(s)	141
Eco57I	CTGAAG	1 cut(s)	1393
Eco81I	CCTNAGG	1 cut(s)	1205
Eco88I	CYCGRG	1 cut(s)	889
EcoICRI	GAGCTC	1 cut(s)	141
EcoO109I	RGGNCCY	1 cut(s)	1138
EcoRII	CCWGG	5 cut(s)	526, 999, 1170, 1219, 1543
EcoRV	GATATC	1 cut(s)	943
EcoT14I	CCWWGG	1 cut(s)	849
EcoT38I	GRGCYC	1 cut(s)	143
EgeI	GGCGCC	1 cut(s)	455
EheI	GGCGCC	1 cut(s)	455
ErhI	CCWWGG	1 cut(s)	849
Esp3I	CGTCTC	3 cut(s)	159, 638, 1497
FaeI	CATG	6 cut(s)	148, 328, 553, 853, 904, 1064
FaqI	GGGAC	1 cut(s)	833
FatI	CATG	6 cut(s)	144, 324, 549, 849, 900, 1060
FauI	CCCGC	2 cut(s)	44, 1236
FauNDI	CATATG	1 cut(s)	541
FokI	GGATG	5 cut(s)	236, 251, 644, 1283, 1462
FriOI	GRGCYC	1 cut(s)	143
FspBI	CTAG	2 cut(s)	1028, 1241
GlaI	GCGC	5 cut(s)	70, 435, 455, 566, 1571
HaeII	RGCGCY	2 cut(s)	72, 457
HaeIII	GGCC	8 cut(s)	562, 721, 848, 1140, 1175, 1224, 1322, 1431
HapII	CCGG	9 cut(s)	171, 194, 206, 248, 330, 457, 667, 1641, 1668
HgaI	GACGC	3 cut(s)	131, 1479, 1682
HhaI	GCGC	5 cut(s)	71, 436, 456, 567, 1572
Hin1I	GRCGYC	2 cut(s)	149, 454
Hin1II	CATG	6 cut(s)	148, 328, 553, 853, 904, 1064
Hin6I	GCGC	5 cut(s)	69, 434, 454, 565, 1570
HinP1I	GCGC	5 cut(s)	69, 434, 454, 565, 1570
HincII	GTYRAC	1 cut(s)	1363
HindII	GTYRAC	1 cut(s)	1363
HindIII	AAGCTT	2 cut(s)	305, 602
HinfI	GANTC	8 cut(s)	83, 244, 345, 488, 583, 686, 1315, 1394
HpaII	CCGG	9 cut(s)	171, 194, 206, 248, 330, 457, 667, 1641, 1668
HphI	GGTGA	5 cut(s)	208, 220, 370, 710, 969
Hpy166II	GTNNAC	1 cut(s)	1363
Hpy188I	TCNGA	8 cut(s)	19, 31, 88, 215, 274, 571, 1015, 1597
Hpy188III	TCNNGA	9 cut(s)	248, 330, 889, 992, 1028, 1289, 1302, 1441, 1679
Hpy8I	GTNNAC	1 cut(s)	1363
Hpy99I	CGWCG	4 cut(s)	19, 125, 154, 1676
HpyCH4III	ACNGT	3 cut(s)	397, 522, 1260
HpyCH4IV	ACGT	2 cut(s)	149, 908
HpyF3I	CTNAG	5 cut(s)	65, 273, 620, 1205, 1649
HpySE526I	ACGT	2 cut(s)	149, 908
Hsp92I	GRCGYC	2 cut(s)	149, 454
Hsp92II	CATG	6 cut(s)	148, 328, 553, 853, 904, 1064
HspAI	GCGC	5 cut(s)	69, 434, 454, 565, 1570
KasI	GGCGCC	1 cut(s)	453
Kpn2I	TCCGGA	2 cut(s)	247, 329
Kzo9I	GATC	1 cut(s)	210
LmnI	GCTCC	3 cut(s)	54, 146, 1681
Lsp1109I	GCAGC	8 cut(s)	108, 111, 603, 812, 820, 1064, 1372, 1400
LweI	GCATC	7 cut(s)	189, 250, 546, 598, 1124, 1219, 1357
MaeI	CTAG	2 cut(s)	1028, 1241
MaeII	ACGT	2 cut(s)	149, 908
MaeIII	GTNAC	1 cut(s)	953
MalI	GATC	1 cut(s)	212
MbiI	CCGCTC	1 cut(s)	46
MboI	GATC	1 cut(s)	210
MboII	GAAGA	7 cut(s)	332, 749, 811, 980, 1096, 1348, 1399
MfeI	CAATTG	1 cut(s)	492
MhlI	GDGCHC	2 cut(s)	143, 164
MluCI	AATT	6 cut(s)	175, 407, 492, 675, 1044, 1284
MluI	ACGCGT	1 cut(s)	1488
Mly113I	GGCGCC	1 cut(s)	454
MlyI	GAGTC	3 cut(s)	92, 354, 1324
MroI	TCCGGA	2 cut(s)	247, 329
MroXI	GAANNNNTTC	3 cut(s)	721, 987, 1166
MseI	TTAA	2 cut(s)	858, 1326
MslI	CAYNNNNRTG	2 cut(s)	554, 1558
MspA1I	CMGCKG	3 cut(s)	427, 1055, 1391
MspI	CCGG	9 cut(s)	171, 194, 206, 248, 330, 457, 667, 1641, 1668
MspR9I	CCNGG	7 cut(s)	458, 528, 668, 1001, 1172, 1221, 1545
MunI	CAATTG	1 cut(s)	492
MvaI	CCWGG	5 cut(s)	528, 1001, 1172, 1221, 1545
MvnI	CGCG	4 cut(s)	125, 565, 1490, 1610
NarI	GGCGCC	1 cut(s)	454
NciI	CCSGG	2 cut(s)	458, 668
NcoI	CCATGG	1 cut(s)	849
NdeI	CATATG	1 cut(s)	541
NdeII	GATC	1 cut(s)	210
NlaIII	CATG	6 cut(s)	148, 328, 553, 853, 904, 1064
NlaIV	GGNNCC	1 cut(s)	455
NotI	GCGGCCGC	1 cut(s)	560
NspI	RCATGY	2 cut(s)	328, 1064
NspV	TTCGAA	1 cut(s)	689
OliI	CACNNNNGTG	1 cut(s)	1558
PceI	AGGCCT	1 cut(s)	721
PciI	ACATGT	1 cut(s)	324
PcsI	WCGNNNNNNNCGW	1 cut(s)	155
PdmI	GAANNNNTTC	3 cut(s)	721, 987, 1166
PfeI	GAWTC	5 cut(s)	244, 488, 583, 686, 1394
Pfl23II	CGTACG	1 cut(s)	1635
PflFI	GACNNNGTC	1 cut(s)	151
PflMI	CCANNNNNTGG	2 cut(s)	1171, 1535
PfoI	TCCNGGA	1 cut(s)	1543
PleI	GAGTC	3 cut(s)	91, 353, 1323
PluTI	GGCGCC	1 cut(s)	457
PpsI	GAGTC	3 cut(s)	91, 353, 1323
PscI	ACATGT	1 cut(s)	324
PshBI	ATTAAT	1 cut(s)	858
Psp124BI	GAGCTC	1 cut(s)	143
Psp1406I	AACGTT	1 cut(s)	908
Psp6I	CCWGG	5 cut(s)	526, 999, 1170, 1219, 1543
PspGI	CCWGG	5 cut(s)	526, 999, 1170, 1219, 1543
PspLI	CGTACG	1 cut(s)	1635
PspN4I	GGNNCC	1 cut(s)	455
PspPI	GGNCC	3 cut(s)	31, 1138, 1174
PstI	CTGCAG	2 cut(s)	967, 1390
PsyI	GACNNNGTC	1 cut(s)	151
PvuII	CAGCTG	1 cut(s)	1055
RsaI	GTAC	2 cut(s)	538, 1637
RsaNI	GTAC	2 cut(s)	537, 1636
RseI	CAYNNNNRTG	2 cut(s)	554, 1558
SacI	GAGCTC	1 cut(s)	143
SaqAI	TTAA	2 cut(s)	858, 1326
Sau3AI	GATC	1 cut(s)	210
Sau96I	GGNCC	3 cut(s)	31, 1138, 1174
SchI	GAGTC	3 cut(s)	92, 354, 1324
ScrFI	CCNGG	7 cut(s)	458, 528, 668, 1001, 1172, 1221, 1545
SduI	GDGCHC	2 cut(s)	143, 164
SfaNI	GCATC	7 cut(s)	189, 250, 546, 598, 1124, 1219, 1357
SfcI	CTRYAG	3 cut(s)	58, 963, 1386
SfoI	GGCGCC	1 cut(s)	455
SfuI	TTCGAA	1 cut(s)	689
SgrAI	CRCCGGYG	1 cut(s)	193
SinI	GGWCC	1 cut(s)	31
SmiMI	CAYNNNNRTG	2 cut(s)	554, 1558
SmlI	CTYRAG	2 cut(s)	1300, 1654
SmoI	CTYRAG	2 cut(s)	1300, 1654
Sse9I	AATT	6 cut(s)	175, 407, 492, 675, 1044, 1284
SseBI	AGGCCT	1 cut(s)	721
SspDI	GGCGCC	1 cut(s)	453
SspMI	CTAG	2 cut(s)	1028, 1241
SstI	GAGCTC	1 cut(s)	143
StuI	AGGCCT	1 cut(s)	721
StyD4I	CCNGG	7 cut(s)	456, 526, 666, 999, 1170, 1219, 1543
StyI	CCWWGG	1 cut(s)	849
TaaI	ACNGT	3 cut(s)	397, 522, 1260
TaiI	ACGT	2 cut(s)	152, 911
TaqI	TCGA	4 cut(s)	120, 137, 689, 725
TasI	AATT	6 cut(s)	175, 407, 492, 675, 1044, 1284
TauI	GCSGC	6 cut(s)	193, 427, 562, 565, 848, 1613
TfiI	GAWTC	5 cut(s)	244, 488, 583, 686, 1394
Tru1I	TTAA	2 cut(s)	858, 1326
Tru9I	TTAA	2 cut(s)	858, 1326
TscAI	CASTG	4 cut(s)	358, 818, 1404, 1579
TseI	GCWGC	8 cut(s)	96, 99, 616, 808, 825, 1052, 1385, 1388
TspDTI	ATGAA	3 cut(s)	302, 558, 1262
TspGWI	ACGGA	2 cut(s)	147, 662
TspRI	CASTG	4 cut(s)	358, 818, 1404, 1579
Tth111I	GACNNNGTC	1 cut(s)	151
Van91I	CCANNNNNTGG	2 cut(s)	1171, 1535
VpaK11BI	GGWCC	1 cut(s)	31
VspI	ATTAAT	1 cut(s)	858
XapI	RAATTY	1 cut(s)	175
XbaI	TCTAGA	1 cut(s)	1027
XceI	RCATGY	2 cut(s)	328, 1064
XmnI	GAANNNNTTC	3 cut(s)	721, 987, 1166
XspI	CTAG	2 cut(s)	1028, 1241
ZraI	GACGTC	1 cut(s)	150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.