Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004730

Physical Location & Seq

Genomic Coordinates Reverse (-)

21194130 .. 21197630

Gene Structure 3501 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1131700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGGGGAGTGTGTCGTCGGAGGACGGCTCGGACCAGCAGAGCGAGCGATGCGGGAGCTACAGCTTAAGCGCCGACGTGAGCGAGTCGGAGAGTTGTAGCAGCTTCTCGTGCCGGAGGTTCGATGCGGAGGGGGCTTCGAGCTCCATGACGTCGTCGCCGCGACCGGGCGGCGGAAATTTCTTCTTTCAGGCGCCGGTGATGCTTCCGGTGATCGGAGGAAAGGATGTGGTGGCTTGGGATGAGAAGCCGGAGAAACGAAATGCTGATTTGTCTGAAGTGGAGATGATGAAGGAGAGGTTTGCCAAGCTTCTGCTTGGAGAAGACATGTCAGGAGGAGGAAAAGGAGTCTGCACTGCCCTTGCTATCTCAAATGCCATCACCAATCTCTCTGCGACGGTGTTTGGCGAATTATGGAGGTTAGAGCCGCTGAAAGCACAGAGAAAGGCAATGTGGCGCCGGGAAATGGACTGGCTGTTATGTGTGAGTGATTCAATTGTTGAGCTTGTGCCTTCGATACAACAGTTTCCGGGTGGGGGGACATACGAAGTCATGGAAATGCGGCCGCGCTCTGACTTGTATGTGAATCTACCTGCCATTAAGAAGCTTGACGCAATGCTGCTTGGTATGCTAGATGGGTTTTGTGAGACAGAGTTCTGCTATGTTGACAGGGGGATTATTTTGGGGGATTCGAAAGAGGGTGAGGGGTTTATTGGTGGAAGGCCTTCAATTAGGCAAGAAGAGAAGTGGTGGCTGCCTTATCCTAAAGTTCCGCCAAATGGGTTGTCCTTTGAAACAAGGAAGAAATTGCAGCAGTGTAGGGACTGCTGCAACCAGATATTGAAGGCAGCCATGGCGATTAATAGTAGTGTGCTTGCTGAGATGGAAATCCCAAGAGCGTACATGGAGTCGTTGCCCAAGAAGGGGAAAGATTGTATGGGCGATATCATTTATCGTTACATAGCTGCAGACCAATTTTCACCAGAGCATCTTCTGGATTGCCTGGACTTGTCCTCAGAACATCAAACTCTAGAGATAGCCAATAGAATTGAGGCAGCTGTGCATGTTTGGGCGCAGAAAGACCAGAATAAACACAATAATCATAAAGCTAAACGGGCATCCTGGGGTGGCAAGGTCAAGGGACTTGTTTCCGATACTGACAAGAGCCATTTTCTGGCCCAACGAGCAGAGACGCTCTTACACAGCCTAAGGCATCGTTTCCCTGGCCTCCCGCAAACTTCACTAGATATGAACAAAATACAATATAACAAGGACGTGGGGCAGTCAATTCTGGAAAGCTACTCAAGAGTTATGGAGAGTCTGGCCTTTAACGTAATGGCAAGGATAGATGATGTCCTCTTCGTTGACGATGCTACCAAGCGATGTGCTGCTGCGGAATCAGTGTCTATCTTCAGCAGGGGAGGTTTGAGTGGCCTTCCTGTCCAGAAGCGGATGTCACCGAGCCCCTTCTCCATTCAACACACTCCTTATGCGTCTCCACTTGCAACACCGGCGTTCTGTTCCTCCACCCCAGTTATTGGAAGTCCAGGAAGGACACCTCTGTGTGTAAAGCGCAACGTTATAATAGATGACAAGTCAGAGAGATTACTCACAGCTGATTTTGAGAGGGTGTGGTCGTACGCCGGAAGCCTAAGCTCAAGAAGAACAACCGGGGATGCTCCTGAACGGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010769 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0031267 GO:0032092 GO:0032501 GO:0032502 GO:0032879 GO:0032989 GO:0040007 GO:0040008 GO:0043393 GO:0043900 GO:0044093 GO:0044425 GO:0044459 GO:0044464 GO:0044706 GO:0045177 GO:0045595 GO:0048468 GO:0048518 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051020 GO:0051049 GO:0051098 GO:0051099 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0065008 GO:0065009 GO:0071840 GO:0071944 GO:0080092 GO:0098590 GO:0098772 GO:1904424 GO:1904426 GO:1904475 GO:1904477 GO:1905097 GO:1905099 GO:2000012 GO:2000241 GO:2001106 GO:2001108

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PRONE

Protein Analysis

564

Amino Acids

62.32

Weight (kDa)

6.09

Isoelectric Point (pI)

51.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRONE	PF03759	91 - 459	2.7e-178	PRONE (Plant-specific Rop nucleotide exchanger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004730)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38430
fragaria_vesca	FvH4_2g15940 FvH4_2g37830 FvH4_2g37830
malus_domestica	MD05G1128400.v1.1 MD08G1005700.v1.1 MD10G1131700.v1.1
prunus_persica	Prupe.1G357600_v2.0.a1 Prupe.8G172500_v2.0.a1
pyrus_communis	pycom05g12430 pycom08g00440 pycom10g11270
rosa_chinensis	RchiOBHm_Chr6g0279461 RchiOBHm_Chr6g0303881
rosa_laevigata	RLG00000011038 RLG00000013145
rosa_multiflora	Rmu_sc0000274.1_g000004 Rmu_sc0004644.1_g000003 Rmu_sc0007179.1_g000004
rosa_roxburghii	Rroxscaffold_7G00164340 Rroxscaffold_7G00188890
rosa_rugosa	Rorug06G0124300 Rorug06G0325500 Rorug06G0325500
rosa_samantha	Rh6AG439800 Rh6BG238000 Rh6BG463200 Rh6CG452100 Rh6DG439000
rosa_wichuraiana	Rw6G020390 Rw6G038080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1580
AatII	GACGTC	1 cut(s)	152
Acc36I	ACCTGC	1 cut(s)	598
AccB1I	GGYRCC	2 cut(s)	190, 453
AccB7I	CCANNNNNTGG	2 cut(s)	1171, 1535
AccII	CGCG	2 cut(s)	160, 565
AclI	AACGTT	1 cut(s)	1575
AcoI	YGGCCR	1 cut(s)	560
AcsI	RAATTY	1 cut(s)	175
AcuI	CTGAAG	2 cut(s)	294, 1393
AcyI	GRCGYC	3 cut(s)	149, 191, 454
AfaI	GTAC	2 cut(s)	899, 1637
AflII	CTTAAG	1 cut(s)	64
AflIII	ACRYGT	1 cut(s)	324
AgsI	TTSAA	5 cut(s)	492, 726, 791, 841, 1475
AjiI	CACGTC	2 cut(s)	76, 1273
AjnI	CCWGG	4 cut(s)	999, 1118, 1219, 1543
AleI	CACNNNNGTG	1 cut(s)	1558
Alw21I	GWGCWC	1 cut(s)	143
Alw26I	GTCTC	3 cut(s)	638, 1181, 1497
AoxI	GGCC	6 cut(s)	560, 719, 1173, 1222, 1320, 1429
ApoI	RAATTY	1 cut(s)	175
ArsI	GACNNNNNNTTYG	2 cut(s)	767, 799
AseI	ATTAAT	1 cut(s)	858
Asp700I	GAANNNNTTC	1 cut(s)	721
AspLEI	GCGC	6 cut(s)	71, 193, 456, 567, 1072, 1572
AspS9I	GGNCC	2 cut(s)	31, 1174
AsuC2I	CCSGG	4 cut(s)	165, 458, 528, 1669
AsuHPI	GGTGA	6 cut(s)	208, 220, 370, 710, 969, 1446
AsuII	TTCGAA	1 cut(s)	689
AvaII	GGWCC	1 cut(s)	31
AxyI	CCTNAGG	1 cut(s)	1205
BaeI	ACNNNNGTAYC	2 cut(s)	506, 539
BanI	GGYRCC	2 cut(s)	190, 453
BanII	GRGCYC	2 cut(s)	143, 1463
BauI	CACGAG	1 cut(s)	106
BbsI	GAAGAC	1 cut(s)	327
Bbv12I	GWGCWC	1 cut(s)	143
BccI	CCATC	3 cut(s)	383, 626, 874
BceAI	ACGGC	1 cut(s)	40
BciT130I	CCWGG	4 cut(s)	1001, 1120, 1221, 1545
BcnI	CCSGG	4 cut(s)	165, 458, 528, 1669
BcoDI	GTCTC	3 cut(s)	638, 1181, 1497
BfaI	CTAG	3 cut(s)	629, 1028, 1241
BfmI	CTRYAG	2 cut(s)	58, 963
BfoI	RGCGCY	3 cut(s)	72, 194, 457
BfrI	CTTAAG	1 cut(s)	64
BfuAI	ACCTGC	1 cut(s)	598
Bme1390I	CCNGG	8 cut(s)	165, 458, 528, 1001, 1120, 1221, 1545, 1669
Bme18I	GGWCC	1 cut(s)	31
BmgBI	CACGTC	2 cut(s)	76, 1273
BmgT120I	GGNCC	2 cut(s)	31, 1174
BmiI	GGNNCC	2 cut(s)	192, 455
BmrFI	CCNGG	8 cut(s)	165, 458, 528, 1001, 1120, 1221, 1545, 1669
BmrI	ACTGGG	1 cut(s)	1523
BmsI	GCATC	8 cut(s)	38, 112, 189, 994, 1124, 1219, 1357, 1663
BmuI	ACTGGG	1 cut(s)	1523
BpiI	GAAGAC	1 cut(s)	327
BplI	GAGNNNNNCTC	2 cut(s)	11, 43
Bpu10I	CCTNAGC	1 cut(s)	1649
Bpu14I	TTCGAA	1 cut(s)	689
BpuEI	CTTGAG	2 cut(s)	1285, 1639
BpuMI	CCSGG	4 cut(s)	165, 458, 528, 1669
BsaBI	GATNNNNATC	1 cut(s)	884
BsaHI	GRCGYC	3 cut(s)	149, 191, 454
BsaJI	CCNNGG	4 cut(s)	849, 1119, 1219, 1668
BsaWI	WCCGGW	1 cut(s)	205
Bse118I	RCCGGY	2 cut(s)	193, 1507
Bse1I	ACTGG	2 cut(s)	473, 1529
Bse21I	CCTNAGG	1 cut(s)	1205
Bse3DI	GCAATG	2 cut(s)	453, 618
Bse8I	GATNNNNATC	1 cut(s)	884
BseBI	CCWGG	4 cut(s)	1001, 1120, 1221, 1545
BseDI	CCNNGG	4 cut(s)	849, 1119, 1219, 1668
BseGI	GGATG	5 cut(s)	229, 244, 1115, 1455, 1678
BseJI	GATNNNNATC	1 cut(s)	884
BseMI	GCAATG	2 cut(s)	453, 618
BseMII	CTCAG	2 cut(s)	867, 1026
BseNI	ACTGG	2 cut(s)	473, 1529
BseRI	GAGGAG	1 cut(s)	348
BseX3I	CGGCCG	1 cut(s)	560
BsgI	GTGCAG	1 cut(s)	334
Bsh1236I	CGCG	2 cut(s)	160, 565
Bsh1285I	CGRYCG	2 cut(s)	164, 563
BshFI	GGCC	6 cut(s)	562, 721, 1175, 1224, 1322, 1431
BshNI	GGYRCC	2 cut(s)	190, 453
BsiEI	CGRYCG	2 cut(s)	164, 563
BsiHKAI	GWGCWC	1 cut(s)	143
BsiWI	CGTACG	1 cut(s)	1635
BslFI	GGGAC	3 cut(s)	550, 833, 1152
BsmAI	GTCTC	3 cut(s)	638, 1181, 1497
BsmBI	CGTCTC	2 cut(s)	1181, 1497
BsmFI	GGGAC	3 cut(s)	550, 833, 1152
BsnI	GGCC	6 cut(s)	562, 721, 1175, 1224, 1322, 1431
Bsp119I	TTCGAA	1 cut(s)	689
Bsp1286I	GDGCHC	2 cut(s)	143, 1463
Bsp143I	GATC	1 cut(s)	210
Bsp19I	CCATGG	1 cut(s)	849
BspANI	GGCC	6 cut(s)	562, 721, 1175, 1224, 1322, 1431
BspCNI	CTCAG	2 cut(s)	868, 1025
BspFNI	CGCG	2 cut(s)	160, 565
BspLI	GGNNCC	2 cut(s)	192, 455
BspMAI	CTGCAG	1 cut(s)	967
BspMI	ACCTGC	1 cut(s)	598
BspT104I	TTCGAA	1 cut(s)	689
BspT107I	GGYRCC	2 cut(s)	190, 453
BspTI	CTTAAG	1 cut(s)	64
BsrDI	GCAATG	2 cut(s)	453, 618
BsrFI	RCCGGY	2 cut(s)	193, 1507
BsrI	ACTGG	2 cut(s)	473, 1529
BssAI	RCCGGY	2 cut(s)	193, 1507
BssECI	CCNNGG	4 cut(s)	849, 1119, 1219, 1668
BssMI	GATC	1 cut(s)	210
BssNI	GRCGYC	3 cut(s)	149, 191, 454
BssSI	CACGAG	1 cut(s)	106
BssT1I	CCWWGG	1 cut(s)	849
Bst2BI	CACGAG	1 cut(s)	106
Bst2UI	CCWGG	4 cut(s)	1001, 1120, 1221, 1545
Bst4CI	ACNGT	2 cut(s)	397, 522
Bst6I	CTCTTC	2 cut(s)	732, 1361
BstACI	GRCGYC	3 cut(s)	149, 191, 454
BstAFI	CTTAAG	1 cut(s)	64
BstBI	TTCGAA	1 cut(s)	689
BstC8I	GCNNGC	2 cut(s)	44, 873
BstDEI	CTNAG	4 cut(s)	876, 1012, 1205, 1649
BstDSI	CCRYGG	1 cut(s)	849
BstF5I	GGATG	5 cut(s)	229, 244, 1115, 1455, 1678
BstFNI	CGCG	2 cut(s)	160, 565
BstH2I	RGCGCY	3 cut(s)	72, 194, 457
BstHHI	GCGC	6 cut(s)	71, 193, 456, 567, 1072, 1572
BstKTI	GATC	1 cut(s)	213
BstMAI	GTCTC	3 cut(s)	638, 1181, 1497
BstMBI	GATC	1 cut(s)	210
BstMCI	CGRYCG	2 cut(s)	164, 563
BstNI	CCWGG	4 cut(s)	1001, 1120, 1221, 1545
BstNSI	RCATGY	2 cut(s)	328, 1064
BstSCI	CCNGG	8 cut(s)	163, 456, 526, 999, 1118, 1219, 1543, 1667
BstSFI	CTRYAG	2 cut(s)	58, 963
BstUI	CGCG	2 cut(s)	160, 565
BstV2I	GAAGAC	1 cut(s)	327
BstZI	CGGCCG	1 cut(s)	560
Bsu36I	CCTNAGG	1 cut(s)	1205
BsuRI	GGCC	6 cut(s)	562, 721, 1175, 1224, 1322, 1431
BtgI	CCRYGG	1 cut(s)	849
BtgZI	GCGATG	2 cut(s)	61, 1393
BtrI	CACGTC	2 cut(s)	76, 1273
BtsCI	GGATG	5 cut(s)	229, 244, 1115, 1455, 1678
BtsI	GCAGTG	2 cut(s)	351, 818
BtsIMutI	CAGTG	3 cut(s)	351, 818, 1404
BveI	ACCTGC	1 cut(s)	598
Cac8I	GCNNGC	2 cut(s)	44, 873
CciNI	GCGGCCGC	1 cut(s)	560
CfoI	GCGC	6 cut(s)	71, 193, 456, 567, 1072, 1572
Cfr10I	RCCGGY	2 cut(s)	193, 1507
Cfr13I	GGNCC	2 cut(s)	31, 1174
CseI	GACGC	3 cut(s)	617, 1198, 1479
Csp6I	GTAC	2 cut(s)	898, 1636
CviAII	CATG	6 cut(s)	145, 325, 550, 850, 901, 1061
CviQI	GTAC	2 cut(s)	898, 1636
DdeI	CTNAG	4 cut(s)	876, 1012, 1205, 1649
DinI	GGCGCC	2 cut(s)	192, 455
DpnI	GATC	1 cut(s)	212
DpnII	GATC	1 cut(s)	210
EaeI	YGGCCR	1 cut(s)	560
EagI	CGGCCG	1 cut(s)	560
Eam1104I	CTCTTC	2 cut(s)	732, 1361
EarI	CTCTTC	2 cut(s)	732, 1361
EciI	GGCGGA	2 cut(s)	186, 759
Ecl136II	GAGCTC	1 cut(s)	141
EclXI	CGGCCG	1 cut(s)	560
Eco130I	CCWWGG	1 cut(s)	849
Eco147I	AGGCCT	1 cut(s)	721
Eco24I	GRGCYC	2 cut(s)	143, 1463
Eco32I	GATATC	1 cut(s)	943
Eco47I	GGWCC	1 cut(s)	31
Eco52I	CGGCCG	1 cut(s)	560
Eco53kI	GAGCTC	1 cut(s)	141
Eco57I	CTGAAG	2 cut(s)	294, 1393
Eco81I	CCTNAGG	1 cut(s)	1205
EcoICRI	GAGCTC	1 cut(s)	141
EcoRII	CCWGG	4 cut(s)	999, 1118, 1219, 1543
EcoRV	GATATC	1 cut(s)	943
EcoT14I	CCWWGG	1 cut(s)	849
EcoT38I	GRGCYC	2 cut(s)	143, 1463
EgeI	GGCGCC	2 cut(s)	192, 455
EheI	GGCGCC	2 cut(s)	192, 455
ErhI	CCWWGG	1 cut(s)	849
Esp3I	CGTCTC	2 cut(s)	1181, 1497
FaeI	CATG	6 cut(s)	148, 328, 553, 853, 904, 1064
FaqI	GGGAC	3 cut(s)	550, 833, 1152
FatI	CATG	6 cut(s)	144, 324, 549, 849, 900, 1060
FauI	CCCGC	2 cut(s)	44, 1236
FokI	GGATG	5 cut(s)	236, 251, 1102, 1462, 1685
FriOI	GRGCYC	2 cut(s)	143, 1463
FspBI	CTAG	3 cut(s)	629, 1028, 1241
GlaI	GCGC	6 cut(s)	70, 192, 455, 566, 1071, 1571
HaeII	RGCGCY	3 cut(s)	72, 194, 457
HaeIII	GGCC	6 cut(s)	562, 721, 1175, 1224, 1322, 1431
HgaI	GACGC	3 cut(s)	617, 1198, 1479
HhaI	GCGC	6 cut(s)	71, 193, 456, 567, 1072, 1572
Hin1I	GRCGYC	3 cut(s)	149, 191, 454
Hin1II	CATG	6 cut(s)	148, 328, 553, 853, 904, 1064
Hin6I	GCGC	6 cut(s)	69, 191, 454, 565, 1070, 1570
HinP1I	GCGC	6 cut(s)	69, 191, 454, 565, 1070, 1570
HincII	GTYRAC	2 cut(s)	664, 1363
HindII	GTYRAC	2 cut(s)	664, 1363
HindIII	AAGCTT	2 cut(s)	305, 602
HinfI	GANTC	8 cut(s)	83, 345, 488, 583, 686, 905, 1315, 1394
HphI	GGTGA	6 cut(s)	208, 220, 370, 710, 969, 1446
Hpy166II	GTNNAC	2 cut(s)	664, 1363
Hpy188I	TCNGA	9 cut(s)	19, 31, 88, 215, 274, 571, 1015, 1150, 1597
Hpy188III	TCNNGA	8 cut(s)	330, 992, 1028, 1289, 1302, 1441, 1656, 1679
Hpy8I	GTNNAC	2 cut(s)	664, 1363
Hpy99I	CGWCG	5 cut(s)	19, 77, 154, 157, 397
HpyAV	CCTTC	9 cut(s)	283, 519, 711, 732, 835, 913, 1442, 1474, 1542
HpyCH4III	ACNGT	2 cut(s)	397, 522
HpyCH4IV	ACGT	5 cut(s)	75, 149, 1272, 1329, 1575
HpyCH4V	TGCA	6 cut(s)	351, 808, 828, 965, 1060, 1502
HpyF3I	CTNAG	4 cut(s)	876, 1012, 1205, 1649
HpySE526I	ACGT	5 cut(s)	75, 149, 1272, 1329, 1575
Hsp92I	GRCGYC	3 cut(s)	149, 191, 454
Hsp92II	CATG	6 cut(s)	148, 328, 553, 853, 904, 1064
HspAI	GCGC	6 cut(s)	69, 191, 454, 565, 1070, 1570
KasI	GGCGCC	2 cut(s)	190, 453
Kzo9I	GATC	1 cut(s)	210
LmnI	GCTCC	3 cut(s)	54, 146, 1681
LweI	GCATC	8 cut(s)	38, 112, 189, 994, 1124, 1219, 1357, 1663
MaeI	CTAG	3 cut(s)	629, 1028, 1241
MaeII	ACGT	5 cut(s)	75, 149, 1272, 1329, 1575
MaeIII	GTNAC	2 cut(s)	953, 1452
MalI	GATC	1 cut(s)	212
MboI	GATC	1 cut(s)	210
MboII	GAAGA	8 cut(s)	172, 332, 749, 811, 980, 1348, 1399, 1671
MfeI	CAATTG	1 cut(s)	492
MhlI	GDGCHC	2 cut(s)	143, 1463
MluCI	AATT	8 cut(s)	175, 407, 492, 726, 803, 971, 1044, 1284
Mly113I	GGCGCC	2 cut(s)	191, 454
MlyI	GAGTC	4 cut(s)	92, 354, 914, 1324
MmeI	TCCRAC	1 cut(s)	66
MroXI	GAANNNNTTC	1 cut(s)	721
MseI	TTAA	4 cut(s)	65, 597, 858, 1326
MslI	CAYNNNNRTG	2 cut(s)	554, 1558
MspA1I	CMGCKG	3 cut(s)	427, 1055, 1613
MspCI	CTTAAG	1 cut(s)	64
MspR9I	CCNGG	8 cut(s)	165, 458, 528, 1001, 1120, 1221, 1545, 1669
MunI	CAATTG	1 cut(s)	492
MvaI	CCWGG	4 cut(s)	1001, 1120, 1221, 1545
MvnI	CGCG	2 cut(s)	160, 565
NarI	GGCGCC	2 cut(s)	191, 454
NciI	CCSGG	4 cut(s)	165, 458, 528, 1669
NcoI	CCATGG	1 cut(s)	849
NdeII	GATC	1 cut(s)	210
NlaIII	CATG	6 cut(s)	148, 328, 553, 853, 904, 1064
NlaIV	GGNNCC	2 cut(s)	192, 455
NmuCI	GTSAC	1 cut(s)	1452
NotI	GCGGCCGC	1 cut(s)	560
NspI	RCATGY	2 cut(s)	328, 1064
NspV	TTCGAA	1 cut(s)	689
OliI	CACNNNNGTG	1 cut(s)	1558
PceI	AGGCCT	1 cut(s)	721
PciI	ACATGT	1 cut(s)	324
PdmI	GAANNNNTTC	1 cut(s)	721
PfeI	GAWTC	4 cut(s)	488, 583, 686, 1394
Pfl23II	CGTACG	1 cut(s)	1635
PflFI	GACNNNGTC	1 cut(s)	151
PflMI	CCANNNNNTGG	2 cut(s)	1171, 1535
PfoI	TCCNGGA	1 cut(s)	1543
PleI	GAGTC	4 cut(s)	91, 353, 913, 1323
PluTI	GGCGCC	2 cut(s)	194, 457
PpsI	GAGTC	4 cut(s)	91, 353, 913, 1323
PscI	ACATGT	1 cut(s)	324
PshBI	ATTAAT	1 cut(s)	858
PsiI	TTATAA	1 cut(s)	1580
Psp124BI	GAGCTC	1 cut(s)	143
Psp1406I	AACGTT	1 cut(s)	1575
Psp6I	CCWGG	4 cut(s)	999, 1118, 1219, 1543
PspGI	CCWGG	4 cut(s)	999, 1118, 1219, 1543
PspLI	CGTACG	1 cut(s)	1635
PspN4I	GGNNCC	2 cut(s)	192, 455
PspPI	GGNCC	2 cut(s)	31, 1174
PstI	CTGCAG	1 cut(s)	967
PsyI	GACNNNGTC	1 cut(s)	151
PvuII	CAGCTG	2 cut(s)	1055, 1613
RsaI	GTAC	2 cut(s)	899, 1637
RsaNI	GTAC	2 cut(s)	898, 1636
RseI	CAYNNNNRTG	2 cut(s)	554, 1558
SacI	GAGCTC	1 cut(s)	143
SaqAI	TTAA	4 cut(s)	65, 597, 858, 1326
Sau3AI	GATC	1 cut(s)	210
Sau96I	GGNCC	2 cut(s)	31, 1174
SchI	GAGTC	4 cut(s)	92, 354, 914, 1324
ScrFI	CCNGG	8 cut(s)	165, 458, 528, 1001, 1120, 1221, 1545, 1669
SduI	GDGCHC	2 cut(s)	143, 1463
SfaNI	GCATC	8 cut(s)	38, 112, 189, 994, 1124, 1219, 1357, 1663
SfcI	CTRYAG	2 cut(s)	58, 963
SfoI	GGCGCC	2 cut(s)	192, 455
SfuI	TTCGAA	1 cut(s)	689
SgrAI	CRCCGGYG	2 cut(s)	193, 1507
SinI	GGWCC	1 cut(s)	31
SmiMI	CAYNNNNRTG	2 cut(s)	554, 1558
SmlI	CTYRAG	3 cut(s)	64, 1300, 1654
SmoI	CTYRAG	3 cut(s)	64, 1300, 1654
Sse9I	AATT	8 cut(s)	175, 407, 492, 726, 803, 971, 1044, 1284
SseBI	AGGCCT	1 cut(s)	721
SspDI	GGCGCC	2 cut(s)	190, 453
SspMI	CTAG	3 cut(s)	629, 1028, 1241
SstI	GAGCTC	1 cut(s)	143
StuI	AGGCCT	1 cut(s)	721
StyD4I	CCNGG	8 cut(s)	163, 456, 526, 999, 1118, 1219, 1543, 1667
StyI	CCWWGG	1 cut(s)	849
TaaI	ACNGT	2 cut(s)	397, 522
TaiI	ACGT	5 cut(s)	78, 152, 1275, 1332, 1578
TaqI	TCGA	4 cut(s)	120, 137, 512, 689
TasI	AATT	8 cut(s)	175, 407, 492, 726, 803, 971, 1044, 1284
TauI	GCSGC	5 cut(s)	160, 171, 427, 562, 565
TfiI	GAWTC	4 cut(s)	488, 583, 686, 1394
Tru1I	TTAA	4 cut(s)	65, 597, 858, 1326
Tru9I	TTAA	4 cut(s)	65, 597, 858, 1326
TscAI	CASTG	3 cut(s)	358, 818, 1404
TseFI	GTSAC	1 cut(s)	1452
Tsp45I	GTSAC	1 cut(s)	1452
TspDTI	ATGAA	2 cut(s)	302, 1262
TspRI	CASTG	3 cut(s)	358, 818, 1404
Tth111I	GACNNNGTC	1 cut(s)	151
Van91I	CCANNNNNTGG	2 cut(s)	1171, 1535
Vha464I	CTTAAG	1 cut(s)	64
VpaK11BI	GGWCC	1 cut(s)	31
VspI	ATTAAT	1 cut(s)	858
XapI	RAATTY	1 cut(s)	175
XbaI	TCTAGA	1 cut(s)	1027
XceI	RCATGY	2 cut(s)	328, 1064
XmnI	GAANNNNTTC	1 cut(s)	721
XspI	CTAG	3 cut(s)	629, 1028, 1241
ZraI	GACGTC	1 cut(s)	150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.