Rh6CG452100

PRONE (Plant-specific Rop nucleotide exchanger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004730
Physical Location & Seq
Reverse (-)
62874612 .. 62877989
3378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG452100.1

Sequence Viewer

Length: 1311 bp
ATGGAGAGCGTCAACGATGAAGAGGTGGAGGACTTGAGCGAGCGGTTCGAGAGCTATAGCTTGAGTGCTGACGTTAGTGAGTCGGAAAGCTCAACTAGTACTAGCTTTTCCTGTCGGAAAAACGAAGCTCATGCTTCTACTTCCATGACTTCCTCGCCGCCCTCCGGACTGGACTTCGCTGACATTTTTTACCGCAAGACGGCCCTGCCGGTCATGTTGCCGGCCTTCGGTGGTGGACGTGATGTTGTAGTTCCGGTGATGACGGAGAAGACTGAGGCTGACTGCTGCATGTCTGAGGTTGAATTGATGAAGGAGAGATTTGCAAAGCTTCTGCTTGGAGAAGATATGTCAGGTGGAGGCAATGGAGTCTGCACTGCTCTTGCTATTTCCAATGCCATCACAAATCTTTCTGCTACTGTGTTTGGAGAGTTATGGAAGTTGGAGCCATTGGCACCGCAGAAGAAGGCAATGTGGTGCCGAGAAATGGAATGGCTTTTGTGTGTGAGTGATTCCATTGTTGAGCTCATACCATCAACTCAAGAATTCCCTGGTGGTGGGACTTTTGAGGTTATGGTCACTCGTCCCCGCTCGGATCTATATGTGAGCCTACCTGCCCTCAGGAAACTGGATGCAATGCTGCTTAGCCTTCTGGATGGATTTAGTGATTCCGAGTTTTATTATGTTGACCGAGGGATAATTGTTGCTGATACTGATGATACCGAAACATTCTCCCGATCTTCCTCTTCGGGTAGACCTTCAATTAGGCAAGAAGAGAAGTGGTGGCTTCCATTCCCTAAAGTACCCCAAAATGGTCTGTCTGAAAATGTGAGGAAGGCATTGCAGCACTGTAGGGAATGCGCACATCAGATCCTTAAGGCCTCCATGGCCATCAATAGTAATGTGCTGGCTGAAATGGAAATCCCAGATGCTTATTTAGAGAGCTTGCCCAAGAGTGGGAAAGCTTGTCTAGGCGAAATCATTTACCGTTTTATAACTGCTGATCGGTTCTCCCCACAGTGTCTTCTTGACTACCTGGACCTCTCATCAGAGTACACCACTCTTGAAATAGCTAACAGGATTGAGGCTGCTGCACACATTTGGAGACAGAAACACCTCAAACGCCATCTGAAGCCTGCGAAATCCGGTAAGTCATCCTGGGGTGGAAAGGTCAAGGGATTGGTTGGTGACATGGATAAATGTAAGCTTCTGGCCTCCCGAGCTGATACCATCTTACAGAATCTAAGGATACGGTTCCCTGGCCTCCCACAGACGGCTCTGGACATGAGCAAGATTCAGTATAACAAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRONE

Protein Analysis

436

Amino Acids

48.41

Weight (kDa)

5.27

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 98 - 435 2.1e-152 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 992
Acc16I TGCGCA 1 cut(s) 859
Acc36I ACCTGC 1 cut(s) 619
AccB1I GGYRCC 2 cut(s) 451, 474
AccBSI CCGCTC 2 cut(s) 43, 588
AccI GTMKAC 1 cut(s) 751
AccIII TCCGGA 1 cut(s) 164
AciI CCGC 5 cut(s) 43, 158, 193, 455, 586
AclWI GGATC 2 cut(s) 600, 862
AcoI YGGCCR 1 cut(s) 885
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 1 cut(s) 1148
AfaI GTAC 3 cut(s) 100, 801, 1052
AfiI CCNNNNNNNGG 9 cut(s) 164, 199, 227, 484, 554, 809, 953, 954, 1270
AflII CTTAAG 1 cut(s) 872
AgsI TTSAA 3 cut(s) 302, 759, 1064
AhlI ACTAGT 1 cut(s) 95
AjiI CACGTC 1 cut(s) 239
AjnI CCWGG 4 cut(s) 547, 1032, 1154, 1255
Alw21I GWGCWC 1 cut(s) 525
Alw26I GTCTC 1 cut(s) 1096
AlwI GGATC 2 cut(s) 600, 862
Ama87I CYCGRG 1 cut(s) 1215
Aor13HI TCCGGA 1 cut(s) 164
AoxI GGCC 6 cut(s) 201, 222, 876, 885, 1209, 1258
ApeKI GCWGC 5 cut(s) 285, 637, 841, 1085, 1088
ApoI RAATTY 1 cut(s) 542
AspLEI GCGC 1 cut(s) 860
AspS9I GGNCC 2 cut(s) 202, 1036
AsuHPI GGTGA 2 cut(s) 268, 1196
AvaI CYCGRG 1 cut(s) 1215
AvaII GGWCC 1 cut(s) 1036
AxyI CCTNAGG 1 cut(s) 617
BalI TGGCCA 1 cut(s) 887
BanI GGYRCC 2 cut(s) 451, 474
BanII GRGCYC 1 cut(s) 525
BbsI GAAGAC 2 cut(s) 275, 1013
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 5 cut(s) 272, 624, 853, 1072, 1075
BccI CCATC 6 cut(s) 404, 538, 647, 896, 1131, 1235
BceAI ACGGC 2 cut(s) 216, 1287
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 4 cut(s) 549, 1034, 1156, 1257
BciVI GTATCC 1 cut(s) 1239
BcoDI GTCTC 1 cut(s) 1096
BcuI ACTAGT 1 cut(s) 95
BfaI CTAG 3 cut(s) 96, 102, 968
BfmI CTRYAG 2 cut(s) 55, 847
BfrI CTTAAG 1 cut(s) 872
BfuAI ACCTGC 1 cut(s) 619
BfuI GTATCC 1 cut(s) 1239
BglI GCCNNNNNGGC 1 cut(s) 884
BisI GCNGC 6 cut(s) 158, 286, 638, 842, 1086, 1089
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 6 cut(s) 159, 287, 639, 843, 1087, 1090
BmcAI AGTACT 1 cut(s) 100
Bme1390I CCNGG 4 cut(s) 549, 1034, 1156, 1257
Bme18I GGWCC 1 cut(s) 1036
BmeT110I CYCGRG 1 cut(s) 1215
BmgBI CACGTC 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 202, 1036
BmiI GGNNCC 4 cut(s) 444, 453, 476, 1253
BmrFI CCNGG 4 cut(s) 549, 1034, 1156, 1257
BmsI GCATC 2 cut(s) 619, 916
BpiI GAAGAC 2 cut(s) 275, 1013
Bpu1102I GCTNAGC 1 cut(s) 641
BpuEI CTTGAG 3 cut(s) 55, 82, 522
BsaJI CCNNGG 5 cut(s) 547, 688, 882, 1155, 1255
BsaWI WCCGGW 3 cut(s) 164, 253, 1142
Bsc4I CCNNNNNNNGG 9 cut(s) 164, 199, 227, 484, 554, 809, 953, 954, 1270
Bse118I RCCGGY 2 cut(s) 208, 220
Bse1I ACTGG 2 cut(s) 174, 630
Bse21I CCTNAGG 1 cut(s) 617
Bse3DI GCAATG 4 cut(s) 367, 474, 639, 836
BseAI TCCGGA 1 cut(s) 164
BseBI CCWGG 4 cut(s) 549, 1034, 1156, 1257
BseDI CCNNGG 5 cut(s) 547, 688, 882, 1155, 1255
BseGI GGATG 3 cut(s) 634, 658, 1151
BseLI CCNNNNNNNGG 9 cut(s) 164, 199, 227, 484, 554, 809, 953, 954, 1270
BseMI GCAATG 4 cut(s) 367, 474, 639, 836
BseMII CTCAG 3 cut(s) 264, 285, 631
BseNI ACTGG 2 cut(s) 174, 630
BseXI GCAGC 5 cut(s) 272, 624, 853, 1072, 1075
BsgI GTGCAG 2 cut(s) 355, 1074
BshFI GGCC 6 cut(s) 203, 224, 878, 887, 1211, 1260
BshNI GGYRCC 2 cut(s) 451, 474
BsiHKAI GWGCWC 1 cut(s) 525
BsiHKCI CYCGRG 1 cut(s) 1215
BsiSI CCGG 5 cut(s) 165, 209, 221, 254, 1143
BslFI GGGAC 2 cut(s) 567, 571
BslI CCNNNNNNNGG 9 cut(s) 164, 199, 227, 484, 554, 809, 953, 954, 1270
BsmAI GTCTC 1 cut(s) 1096
BsmFI GGGAC 2 cut(s) 567, 571
BsmI GAATGC 1 cut(s) 860
BsnI GGCC 6 cut(s) 203, 224, 878, 887, 1211, 1260
BsoBI CYCGRG 1 cut(s) 1215
Bsp1286I GDGCHC 1 cut(s) 525
Bsp13I TCCGGA 1 cut(s) 164
Bsp143I GATC 4 cut(s) 592, 734, 867, 1000
Bsp1720I GCTNAGC 1 cut(s) 641
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 5 cut(s) 43, 158, 193, 455, 586
BspANI GGCC 6 cut(s) 203, 224, 878, 887, 1211, 1260
BspCNI CTCAG 3 cut(s) 265, 286, 630
BspEI TCCGGA 1 cut(s) 164
BspLI GGNNCC 4 cut(s) 444, 453, 476, 1253
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 2 cut(s) 600, 862
BspT107I GGYRCC 2 cut(s) 451, 474
BspTI CTTAAG 1 cut(s) 872
BsrBI CCGCTC 2 cut(s) 43, 588
BsrDI GCAATG 4 cut(s) 367, 474, 639, 836
BsrFI RCCGGY 2 cut(s) 208, 220
BsrI ACTGG 2 cut(s) 174, 630
BssAI RCCGGY 2 cut(s) 208, 220
BssECI CCNNGG 5 cut(s) 547, 688, 882, 1155, 1255
BssMI GATC 4 cut(s) 592, 734, 867, 1000
BssT1I CCWWGG 1 cut(s) 882
Bst2UI CCWGG 4 cut(s) 549, 1034, 1156, 1257
Bst4CI ACNGT 5 cut(s) 418, 848, 986, 1017, 1251
Bst6I CTCTTC 3 cut(s) 15, 748, 765
BstAFI CTTAAG 1 cut(s) 872
BstC8I GCNNGC 5 cut(s) 41, 222, 906, 944, 1134
BstDEI CTNAG 5 cut(s) 273, 294, 617, 641, 1241
BstDSI CCRYGG 1 cut(s) 882
BstF5I GGATG 3 cut(s) 634, 658, 1151
BstHHI GCGC 1 cut(s) 860
BstKTI GATC 4 cut(s) 595, 737, 870, 1003
BstMAI GTCTC 1 cut(s) 1096
BstMBI GATC 4 cut(s) 592, 734, 867, 1000
BstMWI GCNNNNNNNGC 2 cut(s) 884, 1217
BstNI CCWGG 4 cut(s) 549, 1034, 1156, 1257
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 4 cut(s) 547, 1032, 1154, 1255
BstSFI CTRYAG 2 cut(s) 55, 847
BstV1I GCAGC 5 cut(s) 272, 624, 853, 1072, 1075
BstV2I GAAGAC 2 cut(s) 275, 1013
BstX2I RGATCY 2 cut(s) 592, 867
BstYI RGATCY 2 cut(s) 592, 867
Bsu36I CCTNAGG 1 cut(s) 617
BsuI GTATCC 1 cut(s) 1239
BsuRI GGCC 6 cut(s) 203, 224, 878, 887, 1211, 1260
BtgI CCRYGG 1 cut(s) 882
BtrI CACGTC 1 cut(s) 239
BtsCI GGATG 3 cut(s) 634, 658, 1151
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 3 cut(s) 372, 844, 1022
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 5 cut(s) 41, 222, 906, 944, 1134
CfoI GCGC 1 cut(s) 860
Cfr10I RCCGGY 2 cut(s) 208, 220
Cfr13I GGNCC 2 cut(s) 202, 1036
Csp6I GTAC 3 cut(s) 99, 800, 1051
CviAII CATG 7 cut(s) 131, 145, 214, 289, 883, 1189, 1282
CviQI GTAC 3 cut(s) 99, 800, 1051
DdeI CTNAG 5 cut(s) 273, 294, 617, 641, 1241
DpnI GATC 4 cut(s) 594, 736, 869, 1002
DpnII GATC 4 cut(s) 592, 734, 867, 1000
EaeI YGGCCR 1 cut(s) 885
Eam1104I CTCTTC 3 cut(s) 15, 748, 765
EarI CTCTTC 3 cut(s) 15, 748, 765
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 1 cut(s) 882
Eco147I AGGCCT 1 cut(s) 878
Eco24I GRGCYC 1 cut(s) 525
Eco47I GGWCC 1 cut(s) 1036
Eco53kI GAGCTC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 1148
Eco81I CCTNAGG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 1215
EcoICRI GAGCTC 1 cut(s) 523
EcoRI GAATTC 1 cut(s) 542
EcoRII CCWGG 4 cut(s) 547, 1032, 1154, 1255
EcoT14I CCWWGG 1 cut(s) 882
EcoT38I GRGCYC 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 7 cut(s) 134, 148, 217, 292, 886, 1192, 1285
FaqI GGGAC 2 cut(s) 567, 571
FatI CATG 7 cut(s) 130, 144, 213, 288, 882, 1188, 1281
FauI CCCGC 1 cut(s) 593
FblI GTMKAC 1 cut(s) 751
Fnu4HI GCNGC 6 cut(s) 158, 286, 638, 842, 1086, 1089
FokI GGATG 3 cut(s) 641, 665, 1138
FriOI GRGCYC 1 cut(s) 525
Fsp4HI GCNGC 6 cut(s) 158, 286, 638, 842, 1086, 1089
FspAI RTGCGCAY 1 cut(s) 859
FspBI CTAG 3 cut(s) 96, 102, 968
FspI TGCGCA 1 cut(s) 859
GlaI GCGC 1 cut(s) 859
GluI GCNGC 6 cut(s) 158, 286, 638, 842, 1086, 1089
HaeIII GGCC 6 cut(s) 203, 224, 878, 887, 1211, 1260
HapII CCGG 5 cut(s) 165, 209, 221, 254, 1143
HhaI GCGC 1 cut(s) 860
Hin1II CATG 7 cut(s) 134, 148, 217, 292, 886, 1192, 1285
Hin6I GCGC 1 cut(s) 858
HinP1I GCGC 1 cut(s) 858
HincII GTYRAC 2 cut(s) 13, 685
HindII GTYRAC 2 cut(s) 13, 685
HindIII AAGCTT 3 cut(s) 326, 960, 1202
HinfI GANTC 6 cut(s) 80, 366, 509, 665, 1237, 1291
HpaII CCGG 5 cut(s) 165, 209, 221, 254, 1143
HphI GGTGA 2 cut(s) 268, 1196
Hpy166II GTNNAC 5 cut(s) 13, 236, 685, 752, 1053
Hpy188I TCNGA 9 cut(s) 85, 117, 295, 592, 670, 820, 867, 1048, 1128
Hpy8I GTNNAC 5 cut(s) 13, 236, 685, 752, 1053
HpyAV CCTTC 6 cut(s) 235, 304, 457, 656, 765, 826
HpyCH4III ACNGT 5 cut(s) 418, 848, 986, 1017, 1251
HpyCH4IV ACGT 2 cut(s) 72, 238
HpyCH4V TGCA 6 cut(s) 288, 323, 372, 632, 841, 1091
HpyF10VI GCNNNNNNNGC 2 cut(s) 884, 1217
HpyF3I CTNAG 5 cut(s) 273, 294, 617, 641, 1241
HpySE526I ACGT 2 cut(s) 72, 238
Hsp92II CATG 7 cut(s) 134, 148, 217, 292, 886, 1192, 1285
HspAI GCGC 1 cut(s) 858
Kpn2I TCCGGA 1 cut(s) 164
KroI GCCGGC 1 cut(s) 220
KroNI GCCGGC 1 cut(s) 222
Kzo9I GATC 4 cut(s) 592, 734, 867, 1000
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 5 cut(s) 272, 624, 853, 1072, 1075
LweI GCATC 2 cut(s) 619, 916
MaeI CTAG 3 cut(s) 96, 102, 968
MaeII ACGT 2 cut(s) 72, 238
MaeIII GTNAC 2 cut(s) 574, 1184
MalI GATC 4 cut(s) 594, 736, 869, 1002
MbiI CCGCTC 2 cut(s) 43, 588
MboI GATC 4 cut(s) 592, 734, 867, 1000
MboII GAAGA 8 cut(s) 32, 280, 353, 472, 729, 735, 782, 1013
MflI RGATCY 2 cut(s) 592, 867
MhlI GDGCHC 1 cut(s) 525
MlsI TGGCCA 1 cut(s) 887
MluCI AATT 4 cut(s) 302, 542, 696, 759
MluNI TGGCCA 1 cut(s) 887
MlyI GAGTC 2 cut(s) 89, 375
MmeI TCCRAC 3 cut(s) 63, 95, 420
Mox20I TGGCCA 1 cut(s) 887
MroI TCCGGA 1 cut(s) 164
MroNI GCCGGC 1 cut(s) 220
MscI TGGCCA 1 cut(s) 887
MseI TTAA 1 cut(s) 873
Msp20I TGGCCA 1 cut(s) 887
MspCI CTTAAG 1 cut(s) 872
MspI CCGG 5 cut(s) 165, 209, 221, 254, 1143
MspR9I CCNGG 4 cut(s) 549, 1034, 1156, 1257
Mva1269I GAATGC 1 cut(s) 860
MvaI CCWGG 4 cut(s) 549, 1034, 1156, 1257
MwoI GCNNNNNNNGC 2 cut(s) 884, 1217
NaeI GCCGGC 1 cut(s) 222
NcoI CCATGG 1 cut(s) 882
NdeII GATC 4 cut(s) 592, 734, 867, 1000
NgoMIV GCCGGC 1 cut(s) 220
NlaIII CATG 7 cut(s) 134, 148, 217, 292, 886, 1192, 1285
NlaIV GGNNCC 4 cut(s) 444, 453, 476, 1253
NmeAIII GCCGAG 1 cut(s) 503
NmuCI GTSAC 2 cut(s) 574, 1184
NsbI TGCGCA 1 cut(s) 859
NspI RCATGY 1 cut(s) 292
PceI AGGCCT 1 cut(s) 878
PctI GAATGC 1 cut(s) 860
PdiI GCCGGC 1 cut(s) 222
PfeI GAWTC 4 cut(s) 509, 665, 1237, 1291
PkrI GCNGC 6 cut(s) 159, 287, 639, 843, 1087, 1090
PleI GAGTC 2 cut(s) 88, 374
PpsI GAGTC 2 cut(s) 88, 374
PsiI TTATAA 1 cut(s) 992
Psp124BI GAGCTC 1 cut(s) 525
Psp6I CCWGG 4 cut(s) 547, 1032, 1154, 1255
PspGI CCWGG 4 cut(s) 547, 1032, 1154, 1255
PspN4I GGNNCC 4 cut(s) 444, 453, 476, 1253
PspPI GGNCC 2 cut(s) 202, 1036
PsuI RGATCY 2 cut(s) 592, 867
RsaI GTAC 3 cut(s) 100, 801, 1052
RsaNI GTAC 3 cut(s) 99, 800, 1051
SacI GAGCTC 1 cut(s) 525
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 6 cut(s) 158, 286, 638, 842, 1086, 1089
Sau3AI GATC 4 cut(s) 592, 734, 867, 1000
Sau96I GGNCC 2 cut(s) 202, 1036
ScaI AGTACT 1 cut(s) 100
SchI GAGTC 2 cut(s) 89, 375
ScrFI CCNGG 4 cut(s) 549, 1034, 1156, 1257
SduI GDGCHC 1 cut(s) 525
SfaNI GCATC 2 cut(s) 619, 916
SfcI CTRYAG 2 cut(s) 55, 847
SfiI GGCCNNNNNGGCC 1 cut(s) 884
SinI GGWCC 1 cut(s) 1036
SmlI CTYRAG 4 cut(s) 34, 61, 537, 872
SmoI CTYRAG 4 cut(s) 34, 61, 537, 872
SpeI ACTAGT 1 cut(s) 95
Sse9I AATT 4 cut(s) 302, 542, 696, 759
SseBI AGGCCT 1 cut(s) 878
SsiI CCGC 5 cut(s) 43, 158, 193, 455, 586
SspMI CTAG 3 cut(s) 96, 102, 968
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 1 cut(s) 878
StyD4I CCNGG 4 cut(s) 547, 1032, 1154, 1255
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 5 cut(s) 418, 848, 986, 1017, 1251
TaiI ACGT 2 cut(s) 75, 241
TaqI TCGA 1 cut(s) 48
TaqII GACCGA 1 cut(s) 702
TasI AATT 4 cut(s) 302, 542, 696, 759
TatI WGTACW 2 cut(s) 98, 1050
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 4 cut(s) 509, 665, 1237, 1291
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 3 cut(s) 379, 851, 1022
TseFI GTSAC 2 cut(s) 574, 1184
TseI GCWGC 5 cut(s) 285, 637, 841, 1085, 1088
Tsp45I GTSAC 2 cut(s) 574, 1184
TspDTI ATGAA 2 cut(s) 33, 323
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 3 cut(s) 379, 851, 1022
Vha464I CTTAAG 1 cut(s) 872
VpaK11BI GGWCC 1 cut(s) 1036
XapI RAATTY 1 cut(s) 542
XceI RCATGY 1 cut(s) 292
XmiI GTMKAC 1 cut(s) 751
XspI CTAG 3 cut(s) 96, 102, 968
ZrmI AGTACT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.