MD08G1005700.v1.1

PRONE (Plant-specific Rop nucleotide exchanger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004730
Physical Location & Seq
Forward (+)
497265 .. 500337
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1005700.v1.1.491

Sequence Viewer

Length: 1665 bp
ATGGGGAGCACCGGCGTCTCGTCCGAGGATGATGAGTTGGAGCAGTTGAGCGAGCGCTCCGACAATTACAGCTTGAGTGCCGACGTCAGTGAGTCCGAAAGCTCCAGTGGCTTTTCCTCTCGGCAGTACGGTCGCCAAGCTCCTGCTGCTTCCGCTTCTCTCACTTCTTCGCGTCCAACTGGACCCGACTTCGCTGACATTTGTAATTCTCAGCAGCCGCCCATGCAGTTTGTTTTGCCGGCGGTAGGTGGACGACGTGTCGTCCTTCCGCCGTCTATGACGGAGAAACCCCAGACCAAGTTGTCCGAAGTTGAATTGATGAAGGAGCGGTTTGCAAAGCTTCTGCTTGGAGAAGATATGTCCGGCGGAGGCAAGGGAGTCTGTACTGCGCTTGCTATTTCCAATGCAATCACAAATCTTTCTGCCACCGTATTTGGGGAGTTGTGGAAGTTGGAGCCTCTGGCTCCCCAGAAGAAGGCAATGTGGTGCCGAGAGATGGATTGGCTTTTGTCTGTAAGTGATTCTATTGTTGAGCTCATACCATCAGTACAAGATTTCCCTGGTGGTGGGACCTTTGAGGTTATGGTCACTCGGCCACGCTCTGATCTGTATGTAAACCTCCCGGCACTCAAGAAACTGGATGCAATGCTGCTTAGCATTCTTGACGGATTCCACGATTCTGAGTTTTACTATGTTGACCGAGGAATAATTGTTCCTGATTCTGATGATGGTGCCTTGTCTCTCTCTTCCGGCAGACCATCTACTAGGCAAGAAGAGAAATGGTGGCTTCCATTCCCTAAGGTCCCCTCAAATGGTCTGTCGGAAAATGTCAGAAAGACTTTGCAACAGTGTCGGGAATGCACAAATCAGATCCTTAAGGCTGCAATGGCCATCAATAGTAGTGTGCTAGCTGAAATGGAAACCCCAGATGCTTACTTGGAGAGTTTACCCAAGAGTGGGAAAGCTTGTTTAGGCGAAATCATTTACCGTTTTATAGCTGCTGATCGGTTTTCCCCAGAATGTCTTCTTGATTACTTGGACCTCTCATCAGAATACACCACTCTAGAAATAGCAAACAGGATTGAGGCTGCCGCACACATTTGGAAGCAGAAGCATGTGAAACCCCATCTAACTCGTGTAAATTCTGCAAAGTCATCCTGGGGTGGAAAGGTCAAAGGCTTTGTCAGTGACATAGAAAAGAGTAAGCTTCTCGGATATAGGACGGATACCCTCTTACAGAATCTGAAGAGACGGTTCCCTGGCCTCCCACAGACTGCTCTGGACATGAACAAGATTCAGTACAACAGGGATGTGGGGCAGTCAATCCTTGAGAGCTACTCTAGGGTGATGGAACGCATAGCCTTCAACATAATGGCGAGGATAGATGATCTACTATACGTAGACGATGCCACCAAACAGCGGGCAGCTGCAGAGTCTATGTCCCTGTATGACCAGGGAAGGATTGGTGGCACATTGCCAAAACAGAAGCGGATATCACCCAGTCCCTTCTCAATTCACCGTACCTCTTCAATTACATTGCCAGCATTGGATCCTGCTGTGAATCAGATAGGTAGAAGCCCCGGTGGAAGGACACATAATAAACAAACAACGAAGAATAATTTCAGCGACGCGGTGGAAGACAAATTAGAAAAATTAATCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRONE

Protein Analysis

555

Amino Acids

61.22

Weight (kDa)

6.03

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 102 - 472 3.4e-174 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 301
AatII GACGTC 1 cut(s) 87
AccB1I GGYRCC 2 cut(s) 486, 731
AccBSI CCGCTC 1 cut(s) 328
AccI GTMKAC 1 cut(s) 1401
AccII CGCG 2 cut(s) 172, 1631
AclWI GGATC 3 cut(s) 865, 1544, 1557
AcoI YGGCCR 2 cut(s) 593, 888
AcsI RAATTY 1 cut(s) 1141
AcuI CTGAAG 1 cut(s) 1265
AcyI GRCGYC 2 cut(s) 15, 84
AfaI GTAC 5 cut(s) 128, 385, 549, 1301, 1522
AfeI AGCGCT 1 cut(s) 56
AflII CTTAAG 1 cut(s) 875
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 3 cut(s) 314, 1366, 1530
AhdI GACNNNNNGTC 2 cut(s) 257, 260
AjiI CACGTC 1 cut(s) 257
AjnI CCWGG 4 cut(s) 559, 1157, 1258, 1452
AloI GAACNNNNNNTCC 2 cut(s) 696, 728
Alw21I GWGCWC 2 cut(s) 11, 537
Alw26I GTCTC 3 cut(s) 22, 744, 1243
AlwI GGATC 3 cut(s) 865, 1544, 1557
AlwNI CAGNNNCTG 1 cut(s) 1243
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 3 cut(s) 593, 888, 1261
ApeKI GCWGC 8 cut(s) 146, 214, 649, 881, 998, 1088, 1424, 1427
ApoI RAATTY 1 cut(s) 1141
ArsI GACNNNNNNTTYG 4 cut(s) 803, 835, 1167, 1199
AseI ATTAAT 1 cut(s) 1655
Asp700I GAANNNNTTC 2 cut(s) 1023, 1619
AspLEI GCGC 2 cut(s) 57, 391
AspS9I GGNCC 4 cut(s) 182, 570, 802, 1039
AsuC2I CCSGG 2 cut(s) 623, 1581
AsuHPI GGTGA 3 cut(s) 1357, 1488, 1508
AsuNHI GCTAGC 1 cut(s) 907
AvaII GGWCC 4 cut(s) 182, 570, 802, 1039
AxyI CCTNAGG 1 cut(s) 798
BalI TGGCCA 1 cut(s) 890
BamHI GGATCC 1 cut(s) 1549
BanI GGYRCC 2 cut(s) 486, 731
BanII GRGCYC 1 cut(s) 537
BauI CACGAG 1 cut(s) 1134
BbsI GAAGAC 2 cut(s) 1016, 1644
Bbv12I GWGCWC 2 cut(s) 11, 537
BbvI GCAGC 8 cut(s) 133, 226, 636, 868, 985, 1075, 1414, 1436
BccI CCATC 7 cut(s) 490, 550, 722, 766, 899, 1134, 1342
BceAI ACGGC 1 cut(s) 256
BcgI CGANNNNNNTGC 4 cut(s) 113, 147, 833, 867
BciT130I CCWGG 4 cut(s) 561, 1159, 1260, 1454
BciVI GTATCC 1 cut(s) 1219
BcnI CCSGG 2 cut(s) 623, 1581
BcoDI GTCTC 3 cut(s) 22, 744, 1243
BfaI CTAG 4 cut(s) 765, 908, 1064, 1341
BfmI CTRYAG 1 cut(s) 1428
BfoI RGCGCY 1 cut(s) 58
BfrI CTTAAG 1 cut(s) 875
BfuI GTATCC 1 cut(s) 1219
BlpI GCTNAGC 1 cut(s) 653
Bme1390I CCNGG 6 cut(s) 561, 623, 1159, 1260, 1454, 1581
Bme18I GGWCC 4 cut(s) 182, 570, 802, 1039
BmeRI GACNNNNNGTC 2 cut(s) 257, 260
BmgBI CACGTC 1 cut(s) 257
BmgT120I GGNCC 4 cut(s) 182, 570, 802, 1039
BmiI GGNNCC 9 cut(s) 184, 456, 465, 488, 571, 733, 804, 1256, 1551
BmrFI CCNGG 6 cut(s) 561, 623, 1159, 1260, 1454, 1581
BmrI ACTGGG 1 cut(s) 1494
BmsI GCATC 3 cut(s) 631, 919, 1396
BmtI GCTAGC 1 cut(s) 911
BmuI ACTGGG 1 cut(s) 1494
BpiI GAAGAC 2 cut(s) 1016, 1644
BplI GAGNNNNNCTC 2 cut(s) 1322, 1354
BpmI CTGGAG 1 cut(s) 88
Bpu1102I GCTNAGC 1 cut(s) 653
BpuEI CTTGAG 3 cut(s) 94, 614, 1349
BpuMI CCSGG 2 cut(s) 623, 1581
BsaAI YACGTR 1 cut(s) 1399
BsaHI GRCGYC 2 cut(s) 15, 84
BsaJI CCNNGG 7 cut(s) 24, 559, 700, 1158, 1258, 1453, 1579
Bse118I RCCGGY 2 cut(s) 11, 238
Bse1I ACTGG 4 cut(s) 105, 184, 642, 1500
Bse21I CCTNAGG 1 cut(s) 798
Bse3DI GCAATG 5 cut(s) 486, 651, 891, 1472, 1535
BseBI CCWGG 4 cut(s) 561, 1159, 1260, 1454
BseDI CCNNGG 7 cut(s) 24, 559, 700, 1158, 1258, 1453, 1579
BseGI GGATG 4 cut(s) 34, 646, 1154, 1315
BseMI GCAATG 5 cut(s) 486, 651, 891, 1472, 1535
BseMII CTCAG 2 cut(s) 224, 672
BseNI ACTGG 4 cut(s) 105, 184, 642, 1500
BseXI GCAGC 8 cut(s) 133, 226, 636, 868, 985, 1075, 1414, 1436
Bsh1236I CGCG 2 cut(s) 172, 1631
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 3 cut(s) 595, 890, 1263
BshNI GGYRCC 2 cut(s) 486, 731
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 2 cut(s) 11, 537
BsiSI CCGG 6 cut(s) 12, 239, 363, 623, 750, 1581
BslFI GGGAC 4 cut(s) 583, 788, 1426, 1488
BsmAI GTCTC 3 cut(s) 22, 744, 1243
BsmBI CGTCTC 2 cut(s) 22, 1243
BsmFI GGGAC 4 cut(s) 583, 788, 1426, 1488
BsmI GAATGC 2 cut(s) 657, 863
BsnI GGCC 3 cut(s) 595, 890, 1263
Bsp1286I GDGCHC 2 cut(s) 11, 537
Bsp143I GATC 5 cut(s) 604, 870, 1003, 1387, 1549
Bsp1720I GCTNAGC 1 cut(s) 653
BspANI GGCC 3 cut(s) 595, 890, 1263
BspCNI CTCAG 2 cut(s) 223, 673
BspFNI CGCG 2 cut(s) 172, 1631
BspLI GGNNCC 9 cut(s) 184, 456, 465, 488, 571, 733, 804, 1256, 1551
BspMAI CTGCAG 1 cut(s) 1432
BspOI GCTAGC 1 cut(s) 911
BspPI GGATC 3 cut(s) 865, 1544, 1557
BspT107I GGYRCC 2 cut(s) 486, 731
BspTI CTTAAG 1 cut(s) 875
BsrBI CCGCTC 1 cut(s) 328
BsrDI GCAATG 5 cut(s) 486, 651, 891, 1472, 1535
BsrFI RCCGGY 2 cut(s) 11, 238
BsrI ACTGG 4 cut(s) 105, 184, 642, 1500
BssAI RCCGGY 2 cut(s) 11, 238
BssECI CCNNGG 7 cut(s) 24, 559, 700, 1158, 1258, 1453, 1579
BssMI GATC 5 cut(s) 604, 870, 1003, 1387, 1549
BssNI GRCGYC 2 cut(s) 15, 84
BssSI CACGAG 1 cut(s) 1134
Bst2BI CACGAG 1 cut(s) 1134
Bst2UI CCWGG 4 cut(s) 561, 1159, 1260, 1454
Bst4CI ACNGT 6 cut(s) 131, 430, 849, 989, 1254, 1520
Bst6I CTCTTC 4 cut(s) 751, 768, 1241, 1531
BstACI GRCGYC 2 cut(s) 15, 84
BstAFI CTTAAG 1 cut(s) 875
BstBAI YACGTR 1 cut(s) 1399
BstC8I GCNNGC 6 cut(s) 53, 240, 393, 909, 1422, 1542
BstDEI CTNAG 4 cut(s) 210, 653, 681, 798
BstF5I GGATG 4 cut(s) 34, 646, 1154, 1315
BstFNI CGCG 2 cut(s) 172, 1631
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 2 cut(s) 57, 391
BstKTI GATC 5 cut(s) 607, 873, 1006, 1390, 1552
BstMAI GTCTC 3 cut(s) 22, 744, 1243
BstMBI GATC 5 cut(s) 604, 870, 1003, 1387, 1549
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 5 cut(s) 108, 146, 152, 223, 887
BstNI CCWGG 4 cut(s) 561, 1159, 1260, 1454
BstNSI RCATGY 1 cut(s) 1118
BstSCI CCNGG 6 cut(s) 559, 621, 1157, 1258, 1452, 1579
BstSFI CTRYAG 1 cut(s) 1428
BstSNI TACGTA 1 cut(s) 1399
BstUI CGCG 2 cut(s) 172, 1631
BstV1I GCAGC 8 cut(s) 133, 226, 636, 868, 985, 1075, 1414, 1436
BstV2I GAAGAC 2 cut(s) 1016, 1644
BstX2I RGATCY 2 cut(s) 870, 1549
BstYI RGATCY 2 cut(s) 870, 1549
Bsu36I CCTNAGG 1 cut(s) 798
BsuI GTATCC 1 cut(s) 1219
BsuRI GGCC 3 cut(s) 595, 890, 1263
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 4 cut(s) 34, 646, 1154, 1315
BtsIMutI CAGTG 4 cut(s) 94, 112, 854, 1192
Cac8I GCNNGC 6 cut(s) 53, 240, 393, 909, 1422, 1542
CaiI CAGNNNCTG 1 cut(s) 1243
CfoI GCGC 2 cut(s) 57, 391
Cfr10I RCCGGY 2 cut(s) 11, 238
Cfr13I GGNCC 4 cut(s) 182, 570, 802, 1039
CseI GACGC 3 cut(s) 4, 161, 1637
Csp6I GTAC 5 cut(s) 127, 384, 548, 1300, 1521
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviAII CATG 3 cut(s) 223, 1115, 1285
CviQI GTAC 5 cut(s) 127, 384, 548, 1300, 1521
DdeI CTNAG 4 cut(s) 210, 653, 681, 798
DpnI GATC 5 cut(s) 606, 872, 1005, 1389, 1551
DpnII GATC 5 cut(s) 604, 870, 1003, 1387, 1549
DrdI GACNNNNNNGTC 1 cut(s) 301
DriI GACNNNNNGTC 2 cut(s) 257, 260
DseDI GACNNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 2 cut(s) 593, 888
Eam1104I CTCTTC 4 cut(s) 751, 768, 1241, 1531
Eam1105I GACNNNNNGTC 2 cut(s) 257, 260
EarI CTCTTC 4 cut(s) 751, 768, 1241, 1531
EciI GGCGGA 2 cut(s) 258, 381
Ecl136II GAGCTC 1 cut(s) 535
Eco105I TACGTA 1 cut(s) 1399
Eco24I GRGCYC 1 cut(s) 537
Eco32I GATATC 1 cut(s) 1494
Eco47I GGWCC 4 cut(s) 182, 570, 802, 1039
Eco47III AGCGCT 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 1265
Eco81I CCTNAGG 1 cut(s) 798
EcoICRI GAGCTC 1 cut(s) 535
EcoO109I RGGNCCY 2 cut(s) 570, 802
EcoRII CCWGG 4 cut(s) 559, 1157, 1258, 1452
EcoRV GATATC 1 cut(s) 1494
EcoT38I GRGCYC 1 cut(s) 537
Esp3I CGTCTC 2 cut(s) 22, 1243
FaeI CATG 3 cut(s) 226, 1118, 1288
FaqI GGGAC 4 cut(s) 583, 788, 1426, 1488
FatI CATG 3 cut(s) 222, 1114, 1284
FauI CCCGC 1 cut(s) 1413
FblI GTMKAC 1 cut(s) 1401
FokI GGATG 4 cut(s) 41, 653, 1141, 1322
FriOI GRGCYC 1 cut(s) 537
FspBI CTAG 4 cut(s) 765, 908, 1064, 1341
GlaI GCGC 2 cut(s) 56, 390
GsuI CTGGAG 1 cut(s) 88
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 3 cut(s) 595, 890, 1263
HapII CCGG 6 cut(s) 12, 239, 363, 623, 750, 1581
HgaI GACGC 3 cut(s) 4, 161, 1637
HhaI GCGC 2 cut(s) 57, 391
Hin1I GRCGYC 2 cut(s) 15, 84
Hin1II CATG 3 cut(s) 226, 1118, 1288
Hin6I GCGC 2 cut(s) 55, 389
HinP1I GCGC 2 cut(s) 55, 389
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 3 cut(s) 338, 963, 1205
HpaII CCGG 6 cut(s) 12, 239, 363, 623, 750, 1581
HphI GGTGA 3 cut(s) 1357, 1488, 1508
Hpy166II GTNNAC 5 cut(s) 251, 616, 697, 947, 1402
Hpy188III TCNNGA 7 cut(s) 631, 662, 716, 854, 1028, 1064, 1280
Hpy8I GTNNAC 5 cut(s) 251, 616, 697, 947, 1402
Hpy99I CGWCG 3 cut(s) 86, 258, 1631
HpyAV CCTTC 7 cut(s) 275, 316, 469, 1372, 1452, 1516, 1581
HpyCH4III ACNGT 6 cut(s) 131, 430, 849, 989, 1254, 1520
HpyCH4IV ACGT 3 cut(s) 84, 256, 1398
HpyCH4V TGCA 9 cut(s) 226, 335, 407, 644, 844, 861, 884, 1148, 1430
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 146, 152, 223, 887
HpyF3I CTNAG 4 cut(s) 210, 653, 681, 798
HpySE526I ACGT 3 cut(s) 84, 256, 1398
Hsp92I GRCGYC 2 cut(s) 15, 84
Hsp92II CATG 3 cut(s) 226, 1118, 1288
HspAI GCGC 2 cut(s) 55, 389
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Kzo9I GATC 5 cut(s) 604, 870, 1003, 1387, 1549
LmnI GCTCC 8 cut(s) 6, 40, 62, 107, 145, 325, 454, 469
Lsp1109I GCAGC 8 cut(s) 133, 226, 636, 868, 985, 1075, 1414, 1436
LweI GCATC 3 cut(s) 631, 919, 1396
MaeI CTAG 4 cut(s) 765, 908, 1064, 1341
MaeII ACGT 3 cut(s) 84, 256, 1398
MaeIII GTNAC 2 cut(s) 586, 1187
MalI GATC 5 cut(s) 606, 872, 1005, 1389, 1551
MbiI CCGCTC 1 cut(s) 328
MboI GATC 5 cut(s) 604, 870, 1003, 1387, 1549
MflI RGATCY 2 cut(s) 870, 1549
MhlI GDGCHC 2 cut(s) 11, 537
MlsI TGGCCA 1 cut(s) 890
MluNI TGGCCA 1 cut(s) 890
MlyI GAGTC 3 cut(s) 101, 387, 1442
MmeI TCCRAC 5 cut(s) 18, 84, 200, 432, 801
Mox20I TGGCCA 1 cut(s) 890
MroNI GCCGGC 1 cut(s) 238
MroXI GAANNNNTTC 2 cut(s) 1023, 1619
MscI TGGCCA 1 cut(s) 890
MseI TTAA 2 cut(s) 876, 1655
Msp20I TGGCCA 1 cut(s) 890
MspA1I CMGCKG 2 cut(s) 1420, 1427
MspCI CTTAAG 1 cut(s) 875
MspI CCGG 6 cut(s) 12, 239, 363, 623, 750, 1581
MspR9I CCNGG 6 cut(s) 561, 623, 1159, 1260, 1454, 1581
Mva1269I GAATGC 2 cut(s) 657, 863
MvaI CCWGG 4 cut(s) 561, 1159, 1260, 1454
MvnI CGCG 2 cut(s) 172, 1631
MwoI GCNNNNNNNGC 5 cut(s) 108, 146, 152, 223, 887
NaeI GCCGGC 1 cut(s) 240
NciI CCSGG 2 cut(s) 623, 1581
NdeII GATC 5 cut(s) 604, 870, 1003, 1387, 1549
NgoMIV GCCGGC 1 cut(s) 238
NheI GCTAGC 1 cut(s) 907
NlaIII CATG 3 cut(s) 226, 1118, 1288
NlaIV GGNNCC 9 cut(s) 184, 456, 465, 488, 571, 733, 804, 1256, 1551
NmeAIII GCCGAG 3 cut(s) 100, 515, 571
NmuCI GTSAC 2 cut(s) 586, 1187
NspI RCATGY 1 cut(s) 1118
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PctI GAATGC 2 cut(s) 657, 863
PdiI GCCGGC 1 cut(s) 240
PdmI GAANNNNTTC 2 cut(s) 1023, 1619
PfeI GAWTC 7 cut(s) 521, 669, 677, 719, 1240, 1294, 1561
PleI GAGTC 3 cut(s) 100, 386, 1441
PpsI GAGTC 3 cut(s) 100, 386, 1441
Ppu21I YACGTR 1 cut(s) 1399
PpuMI RGGWCCY 2 cut(s) 570, 802
PshBI ATTAAT 1 cut(s) 1655
Psp124BI GAGCTC 1 cut(s) 537
Psp5II RGGWCCY 2 cut(s) 570, 802
Psp6I CCWGG 4 cut(s) 559, 1157, 1258, 1452
PspGI CCWGG 4 cut(s) 559, 1157, 1258, 1452
PspN4I GGNNCC 9 cut(s) 184, 456, 465, 488, 571, 733, 804, 1256, 1551
PspPI GGNCC 4 cut(s) 182, 570, 802, 1039
PspPPI RGGWCCY 2 cut(s) 570, 802
PstI CTGCAG 1 cut(s) 1432
PstNI CAGNNNCTG 1 cut(s) 1243
PsuI RGATCY 2 cut(s) 870, 1549
PvuII CAGCTG 1 cut(s) 1427
RsaI GTAC 5 cut(s) 128, 385, 549, 1301, 1522
RsaNI GTAC 5 cut(s) 127, 384, 548, 1300, 1521
SacI GAGCTC 1 cut(s) 537
SaqAI TTAA 2 cut(s) 876, 1655
Sau3AI GATC 5 cut(s) 604, 870, 1003, 1387, 1549
Sau96I GGNCC 4 cut(s) 182, 570, 802, 1039
SchI GAGTC 3 cut(s) 101, 387, 1442
ScrFI CCNGG 6 cut(s) 561, 623, 1159, 1260, 1454, 1581
SduI GDGCHC 2 cut(s) 11, 537
SfaNI GCATC 3 cut(s) 631, 919, 1396
SfcI CTRYAG 1 cut(s) 1428
SgrAI CRCCGGYG 1 cut(s) 11
SinI GGWCC 4 cut(s) 182, 570, 802, 1039
SmlI CTYRAG 4 cut(s) 73, 629, 875, 1328
SmoI CTYRAG 4 cut(s) 73, 629, 875, 1328
SnaBI TACGTA 1 cut(s) 1399
SspMI CTAG 4 cut(s) 765, 908, 1064, 1341
SstI GAGCTC 1 cut(s) 537
StyD4I CCNGG 6 cut(s) 559, 621, 1157, 1258, 1452, 1579
TaaI ACNGT 6 cut(s) 131, 430, 849, 989, 1254, 1520
TaiI ACGT 3 cut(s) 87, 259, 1401
TaqII GACCGA 1 cut(s) 714
TatI WGTACW 3 cut(s) 383, 547, 1299
TauI GCSGC 2 cut(s) 220, 1094
TfiI GAWTC 7 cut(s) 521, 669, 677, 719, 1240, 1294, 1561
Tru1I TTAA 2 cut(s) 876, 1655
Tru9I TTAA 2 cut(s) 876, 1655
TscAI CASTG 4 cut(s) 94, 112, 854, 1192
TseFI GTSAC 2 cut(s) 586, 1187
TseI GCWGC 8 cut(s) 146, 214, 649, 881, 998, 1088, 1424, 1427
Tsp45I GTSAC 2 cut(s) 586, 1187
TspDTI ATGAA 2 cut(s) 335, 1301
TspGWI ACGGA 3 cut(s) 296, 681, 1238
TspRI CASTG 4 cut(s) 94, 112, 854, 1192
Vha464I CTTAAG 1 cut(s) 875
VpaK11BI GGWCC 4 cut(s) 182, 570, 802, 1039
VspI ATTAAT 1 cut(s) 1655
XapI RAATTY 1 cut(s) 1141
XbaI TCTAGA 1 cut(s) 1063
XceI RCATGY 1 cut(s) 1118
XcmI CCANNNNNNNNNTGG 1 cut(s) 1460
XmiI GTMKAC 1 cut(s) 1401
XmnI GAANNNNTTC 2 cut(s) 1023, 1619
XspI CTAG 4 cut(s) 765, 908, 1064, 1341
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.