MD05G1129000.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008796
Physical Location & Seq
Forward (+)
25306531 .. 25310547
4017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1129000.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCACCAAAAGTCGGATTCGGATGTGACGATGAGCGTGGAGCAGTTGACACCGCCGCGGTCGCCTCGCCGTCCGATTTACTACGTCCAGAGCCCCTCCAACCACGACGTCGAGAAGATGTCGTACGGCTCGAGTCCTGTTGGGTCGCCGCAGCACCACTACTATCACTGCTCCCCTATCCACCACTCCCGCGAGTCCTCCACGTCGAGATTCTCCGCCTCCCTGAAAAACCCCCGCAGCATGTCCGCCTGGCGGAAGCTGCAGCGCGGGGAAGAAGTGGAATTGTCCGAAGACGACGAAAATGACTCCGCATTCGACGACGGCGGCCCCGCGCGTAATATTAGGTTGTACTTCTGCTTCGCGCTGCTTTTCGTTGTGCTCTTCACGGTGTTTTCTTTGATCTTGTGGGGCGCCAGCAAGGCTTACGAGCCTCGAGTCATCGTTAAGAATATTGTGTTCGAGAGTCTAAATATACAGGCGGGGAGCGACATGACGGGGGTCTCGACGGACATGCTGTCGTTGAATTCGACGGTGAGGATCTCTTACAGGAATCCCGCGACATTCTTCGGCGTCCACGTCACGTCGACTCCATTGGAGCTCCACTATTACCAGCTCAAGGTTGCGTCCGGGCAGGTTGAAAACTTCTACCAAGCAAGGAAGACGCATCGGACAATAAGGACGGTTGTGCTAGGGTACCAAGTGGCTCTTTACGGGGGAATTTCAGTTATTCAAGATGTTAAAGAGGATCGGCAGAGAGTGGTAATACCGCTGAATCTGACGTTTGTTGTGAGGTCAAGAGCCTACATTTTGGGAAAACTAGTGAAGTCCAAGTTTTATGAGAGAATCAGATGCTCTGTCACTCTTAGAGGAAGCACACTAGGCAAGCACTCAAATTTGACAGATTATTGTGTCTACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

307

Amino Acids

34.78

Weight (kDa)

9.24

Isoelectric Point (pI)

55.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 111
Acc36I ACCTGC 1 cut(s) 622
Acc65I GGTACC 1 cut(s) 693
AccB1I GGYRCC 2 cut(s) 410, 693
AccI GTMKAC 2 cut(s) 584, 912
AccII CGCG 7 cut(s) 58, 192, 267, 332, 334, 362, 557
AclWI GGATC 2 cut(s) 545, 753
AcsI RAATTY 3 cut(s) 523, 717, 892
AcyI GRCGYC 3 cut(s) 108, 411, 570
AfaI GTAC 3 cut(s) 125, 350, 695
AfiI CCNNNNNNNGG 3 cut(s) 13, 252, 616
AgsI TTSAA 3 cut(s) 523, 638, 731
AhdI GACNNNNNGTC 1 cut(s) 514
AhlI ACTAGT 1 cut(s) 817
AjiI CACGTC 3 cut(s) 204, 577, 582
AjnI CCWGG 1 cut(s) 248
AluBI AGCT 3 cut(s) 259, 598, 613
AluI AGCT 3 cut(s) 259, 598, 613
Alw21I GWGCWC 2 cut(s) 381, 600
Alw26I GTCTC 1 cut(s) 505
AlwI GGATC 2 cut(s) 545, 753
Ama87I CYCGRG 2 cut(s) 130, 432
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 5 cut(s) 151, 237, 259, 262, 364
ApoI RAATTY 3 cut(s) 523, 717, 892
ArsI GACNNNNNNTTYG 2 cut(s) 296, 328
Asp718I GGTACC 1 cut(s) 693
AspLEI GCGC 4 cut(s) 267, 334, 364, 413
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 628
AsuHPI GGTGA 1 cut(s) 544
AvaI CYCGRG 2 cut(s) 130, 432
BanI GGYRCC 2 cut(s) 410, 693
BanII GRGCYC 2 cut(s) 95, 600
BarI GAAGNNNNNNTAC 4 cut(s) 107, 139, 341, 373
BbsI GAAGAC 2 cut(s) 297, 665
Bbv12I GWGCWC 2 cut(s) 381, 600
BbvI GCAGC 5 cut(s) 163, 246, 249, 274, 351
BceAI ACGGC 3 cut(s) 54, 142, 337
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 1 cut(s) 250
BcnI CCSGG 1 cut(s) 628
BcoDI GTCTC 1 cut(s) 505
BcuI ACTAGT 1 cut(s) 817
BfaI CTAG 3 cut(s) 689, 818, 878
BfmI CTRYAG 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 414
BfuAI ACCTGC 1 cut(s) 622
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 8 cut(s) 56, 149, 152, 238, 260, 263, 325, 365
BlsI GCNGC 8 cut(s) 57, 150, 153, 239, 261, 264, 326, 366
Bme1390I CCNGG 2 cut(s) 250, 628
BmeRI GACNNNNNGTC 1 cut(s) 514
BmeT110I CYCGRG 2 cut(s) 130, 432
BmgBI CACGTC 3 cut(s) 204, 577, 582
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 3 cut(s) 328, 412, 695
BmrFI CCNGG 2 cut(s) 250, 628
BmsI GCATC 2 cut(s) 673, 839
BoxI GACNNNNGTC 1 cut(s) 497
BpiI GAAGAC 2 cut(s) 297, 665
BpuEI CTTGAG 1 cut(s) 599
BpuMI CCSGG 1 cut(s) 628
BsaHI GRCGYC 3 cut(s) 108, 411, 570
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 571, 601
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 252, 616
BseBI CCWGG 1 cut(s) 250
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 3 cut(s) 13, 252, 616
BseXI GCAGC 5 cut(s) 163, 246, 249, 274, 351
Bsh1236I CGCG 7 cut(s) 58, 192, 267, 332, 334, 362, 557
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 327
BshNI GGYRCC 2 cut(s) 410, 693
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 381, 600
BsiHKCI CYCGRG 2 cut(s) 130, 432
BsiSI CCGG 1 cut(s) 627
BsiWI CGTACG 1 cut(s) 123
BslI CCNNNNNNNGG 3 cut(s) 13, 252, 616
BsmAI GTCTC 1 cut(s) 505
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 1 cut(s) 327
Bso31I GGTCTC 1 cut(s) 505
BsoBI CYCGRG 2 cut(s) 130, 432
Bsp1286I GDGCHC 3 cut(s) 95, 381, 600
Bsp143I GATC 3 cut(s) 399, 537, 745
BspANI GGCC 1 cut(s) 327
BspFNI CGCG 7 cut(s) 58, 192, 267, 332, 334, 362, 557
BspLI GGNNCC 3 cut(s) 328, 412, 695
BspMAI CTGCAG 1 cut(s) 264
BspMI ACCTGC 1 cut(s) 622
BspPI GGATC 2 cut(s) 545, 753
BspQI GCTCTTC 1 cut(s) 386
BspT107I GGYRCC 2 cut(s) 410, 693
BspTNI GGTCTC 1 cut(s) 505
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 3 cut(s) 399, 537, 745
BssNI GRCGYC 3 cut(s) 108, 411, 570
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 3 cut(s) 388, 532, 682
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 3 cut(s) 108, 411, 570
BstC8I GCNNGC 2 cut(s) 415, 884
BstDEI CTNAG 1 cut(s) 863
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 28
BstFNI CGCG 7 cut(s) 58, 192, 267, 332, 334, 362, 557
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 4 cut(s) 267, 334, 364, 413
BstKTI GATC 3 cut(s) 402, 540, 748
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 3 cut(s) 399, 537, 745
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 4 cut(s) 61, 259, 419, 879
BstNI CCWGG 1 cut(s) 250
BstNSI RCATGY 2 cut(s) 244, 514
BstPAI GACNNNNGTC 1 cut(s) 497
BstSCI CCNGG 2 cut(s) 248, 626
BstSFI CTRYAG 1 cut(s) 260
BstUI CGCG 7 cut(s) 58, 192, 267, 332, 334, 362, 557
BstV1I GCAGC 5 cut(s) 163, 246, 249, 274, 351
BstV2I GAAGAC 2 cut(s) 297, 665
BstX2I RGATCY 1 cut(s) 537
BstYI RGATCY 1 cut(s) 537
BsuRI GGCC 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 56
BtrI CACGTC 3 cut(s) 204, 577, 582
BtsCI GGATG 1 cut(s) 28
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 166
BveI ACCTGC 1 cut(s) 622
Cac8I GCNNGC 2 cut(s) 415, 884
CfoI GCGC 4 cut(s) 267, 334, 364, 413
Cfr13I GGNCC 1 cut(s) 326
Cfr42I CCGCGG 1 cut(s) 59
CseI GACGC 3 cut(s) 559, 612, 670
Csp6I GTAC 3 cut(s) 124, 349, 694
CviAII CATG 3 cut(s) 241, 490, 511
CviQI GTAC 3 cut(s) 124, 349, 694
DdeI CTNAG 1 cut(s) 863
DinI GGCGCC 1 cut(s) 412
DpnI GATC 3 cut(s) 401, 539, 747
DpnII GATC 3 cut(s) 399, 537, 745
DriI GACNNNNNGTC 1 cut(s) 514
Eam1104I CTCTTC 1 cut(s) 386
Eam1105I GACNNNNNGTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 3 cut(s) 205, 235, 268
Ecl136II GAGCTC 1 cut(s) 598
Eco24I GRGCYC 2 cut(s) 95, 600
Eco31I GGTCTC 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 598
Eco88I CYCGRG 2 cut(s) 130, 432
EcoICRI GAGCTC 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 248
EcoT38I GRGCYC 2 cut(s) 95, 600
EgeI GGCGCC 1 cut(s) 412
EheI GGCGCC 1 cut(s) 412
FaeI CATG 3 cut(s) 244, 493, 514
FaiI YATR 5 cut(s) 242, 473, 491, 512, 837
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 3 cut(s) 240, 489, 510
FauI CCCGC 6 cut(s) 197, 242, 260, 337, 472, 562
FblI GTMKAC 2 cut(s) 584, 912
Fnu4HI GCNGC 8 cut(s) 56, 149, 152, 238, 260, 263, 325, 365
FokI GGATG 1 cut(s) 35
FriOI GRGCYC 2 cut(s) 95, 600
Fsp4HI GCNGC 8 cut(s) 56, 149, 152, 238, 260, 263, 325, 365
FspBI CTAG 3 cut(s) 689, 818, 878
GlaI GCGC 4 cut(s) 266, 333, 363, 412
GluI GCNGC 8 cut(s) 56, 149, 152, 238, 260, 263, 325, 365
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 1 cut(s) 327
HapII CCGG 1 cut(s) 627
HgaI GACGC 3 cut(s) 559, 612, 670
HhaI GCGC 4 cut(s) 267, 334, 364, 413
Hin1I GRCGYC 3 cut(s) 108, 411, 570
Hin1II CATG 3 cut(s) 244, 493, 514
Hin6I GCGC 4 cut(s) 265, 332, 362, 411
HinP1I GCGC 4 cut(s) 265, 332, 362, 411
HincII GTYRAC 2 cut(s) 48, 585
HindII GTYRAC 2 cut(s) 48, 585
HpaII CCGG 1 cut(s) 627
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 4 cut(s) 48, 574, 585, 913
Hpy188I TCNGA 7 cut(s) 16, 22, 75, 289, 669, 777, 848
Hpy188III TCNNGA 7 cut(s) 88, 112, 207, 460, 502, 731, 795
Hpy8I GTNNAC 4 cut(s) 48, 574, 585, 913
Hpy99I CGWCG 9 cut(s) 110, 113, 208, 299, 320, 323, 508, 532, 586
HpyCH4III ACNGT 3 cut(s) 388, 532, 682
HpyCH4IV ACGT 6 cut(s) 84, 108, 203, 576, 581, 779
HpyCH4V TGCA 2 cut(s) 4, 262
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 259, 419, 879
HpyF3I CTNAG 1 cut(s) 863
HpySE526I ACGT 6 cut(s) 84, 108, 203, 576, 581, 779
Hsp92I GRCGYC 3 cut(s) 108, 411, 570
Hsp92II CATG 3 cut(s) 244, 493, 514
HspAI GCGC 4 cut(s) 265, 332, 362, 411
KasI GGCGCC 1 cut(s) 410
KpnI GGTACC 1 cut(s) 697
KspI CCGCGG 1 cut(s) 59
Kzo9I GATC 3 cut(s) 399, 537, 745
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 5 cut(s) 40, 176, 483, 595, 603
Lsp1109I GCAGC 5 cut(s) 163, 246, 249, 274, 351
LweI GCATC 2 cut(s) 673, 839
MaeI CTAG 3 cut(s) 689, 818, 878
MaeII ACGT 6 cut(s) 84, 108, 203, 576, 581, 779
MaeIII GTNAC 3 cut(s) 25, 577, 856
MalI GATC 3 cut(s) 401, 539, 747
MboI GATC 3 cut(s) 399, 537, 745
MboII GAAGA 6 cut(s) 127, 284, 302, 373, 556, 670
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 3 cut(s) 95, 381, 600
MluCI AATT 4 cut(s) 281, 523, 717, 892
Mly113I GGCGCC 1 cut(s) 411
MlyI GAGTC 6 cut(s) 142, 203, 299, 444, 472, 580
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 9 cut(s) 75, 106, 208, 229, 441, 528, 736, 783, 860
MseI TTAA 2 cut(s) 444, 738
MspA1I CMGCKG 2 cut(s) 58, 769
MspI CCGG 1 cut(s) 627
MspR9I CCNGG 2 cut(s) 250, 628
Mva1269I GAATGC 1 cut(s) 311
MvaI CCWGG 1 cut(s) 250
MvnI CGCG 7 cut(s) 58, 192, 267, 332, 334, 362, 557
MwoI GCNNNNNNNGC 4 cut(s) 61, 259, 419, 879
NarI GGCGCC 1 cut(s) 411
NciI CCSGG 1 cut(s) 628
NdeII GATC 3 cut(s) 399, 537, 745
NlaIII CATG 3 cut(s) 244, 493, 514
NlaIV GGNNCC 3 cut(s) 328, 412, 695
NmuCI GTSAC 3 cut(s) 25, 577, 856
NspI RCATGY 2 cut(s) 244, 514
PaeR7I CTCGAG 2 cut(s) 130, 432
PciSI GCTCTTC 1 cut(s) 386
PcsI WCGNNNNNNNCGW 5 cut(s) 26, 128, 500, 524, 573
PctI GAATGC 1 cut(s) 311
PfeI GAWTC 5 cut(s) 17, 210, 550, 772, 843
Pfl23II CGTACG 1 cut(s) 123
PkrI GCNGC 8 cut(s) 57, 150, 153, 239, 261, 264, 326, 366
PleI GAGTC 6 cut(s) 141, 202, 299, 443, 471, 580
PluTI GGCGCC 1 cut(s) 414
PpsI GAGTC 6 cut(s) 141, 202, 299, 443, 471, 580
PshAI GACNNNNGTC 1 cut(s) 497
Psp124BI GAGCTC 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 248
PspGI CCWGG 1 cut(s) 248
PspLI CGTACG 1 cut(s) 123
PspN4I GGNNCC 3 cut(s) 328, 412, 695
PspPI GGNCC 1 cut(s) 326
PspXI VCTCGAGB 2 cut(s) 130, 432
PstI CTGCAG 1 cut(s) 264
PsuI RGATCY 1 cut(s) 537
RsaI GTAC 3 cut(s) 125, 350, 695
RsaNI GTAC 3 cut(s) 124, 349, 694
SacI GAGCTC 1 cut(s) 600
SacII CCGCGG 1 cut(s) 59
SalI GTCGAC 1 cut(s) 583
SapI GCTCTTC 1 cut(s) 386
SaqAI TTAA 2 cut(s) 444, 738
SatI GCNGC 8 cut(s) 56, 149, 152, 238, 260, 263, 325, 365
Sau3AI GATC 3 cut(s) 399, 537, 745
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 6 cut(s) 142, 203, 299, 444, 472, 580
ScrFI CCNGG 2 cut(s) 250, 628
SduI GDGCHC 3 cut(s) 95, 381, 600
SfaNI GCATC 2 cut(s) 673, 839
SfcI CTRYAG 1 cut(s) 260
SfoI GGCGCC 1 cut(s) 412
Sfr274I CTCGAG 2 cut(s) 130, 432
Sfr303I CCGCGG 1 cut(s) 59
SgrBI CCGCGG 1 cut(s) 59
SlaI CTCGAG 2 cut(s) 130, 432
SmlI CTYRAG 3 cut(s) 130, 432, 614
SmoI CTYRAG 3 cut(s) 130, 432, 614
SpeI ACTAGT 1 cut(s) 817
Sse9I AATT 4 cut(s) 281, 523, 717, 892
SspDI GGCGCC 1 cut(s) 410
SspI AATATT 2 cut(s) 340, 451
SspMI CTAG 3 cut(s) 689, 818, 878
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 2 cut(s) 248, 626
TaaI ACNGT 3 cut(s) 388, 532, 682
TaiI ACGT 6 cut(s) 87, 111, 206, 579, 584, 782
TaqI TCGA 9 cut(s) 111, 131, 206, 315, 433, 459, 503, 527, 584
TasI AATT 4 cut(s) 281, 523, 717, 892
TatI WGTACW 1 cut(s) 348
TauI GCSGC 3 cut(s) 58, 151, 327
TfiI GAWTC 5 cut(s) 17, 210, 550, 772, 843
Tru1I TTAA 2 cut(s) 444, 738
Tru9I TTAA 2 cut(s) 444, 738
TscAI CASTG 1 cut(s) 173
TseFI GTSAC 3 cut(s) 25, 577, 856
TseI GCWGC 5 cut(s) 151, 237, 259, 262, 364
Tsp45I GTSAC 3 cut(s) 25, 577, 856
TspGWI ACGGA 1 cut(s) 521
TspRI CASTG 1 cut(s) 173
XapI RAATTY 3 cut(s) 523, 717, 892
XceI RCATGY 2 cut(s) 244, 514
XhoI CTCGAG 2 cut(s) 130, 432
XmiI GTMKAC 2 cut(s) 584, 912
XspI CTAG 3 cut(s) 689, 818, 878
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.