Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010996

Physical Location & Seq

Genomic Coordinates Reverse (-)

25713219 .. 25718884

Gene Structure 5666 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1131700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2442 bp

ATGGGTTGCACGAGCTCCAAGCTCGACGAACTTCCGGCCGTTGCCTTATGCCGGGAGCGCTGCGGCTTCCTCGACGAGGCGATTCACCAGCGCTACGCCCTCGCCGAGGCCCACATCGCTTATATTAACTCCCTCAGAGGAATCGGCCGCTCTCTGCATCAATTCATCGAGCAGGAGGTGGGCAATTCATCCGGGTCGCCGCCCTCTCCCCACCTCAACCTCCCGCCGGCCAGAAAAGGCGAGATCAAATCCTCGCCGCCGCGCCACTCTCACTCAAATTCAAATTCCGGGTCCCATCTCCACTTTCGCTCCGATTCCGACAACGATTCCGGCTCGCTCCACGACTCCGGCCACTCGTCGCCACTGCACGATACTCACTACGGCGGTCATATTGATTACATGGAGGAGGGCGGGCAAGAGGGTTTCAATTCTTACCCAGATGGGTTTATGCGCATGAATTACATGAAGAACAAAGCGACGCCATCTGTGGTGCATCGGCAGAAACCCGTGAGCCCCGAAAACGTGTTTCAATTGGGGGAATCTTCTTCTTCGAATTCGTATTATGGATATCAAAATTCAAATATTTATCCAGACGCTGTCTCTAATCCTTATTCCGATTTCGGCGGTGGGTTTAACAATTACGGTGGTGCGTATAATTACGGCGGAGGGAGTGGTGGGTATAACAATTACGGGAATTCTTCTCCGCTGGTCCGATATGGAGCACCGTCGCCACAGGCTTCAGCTTCGACCTCGTCTAAGCCACCTCCTCCGCCGCCTTCGCCGCCAAGAGCTTCAGCTTGGGATTTTTTAAACCCATTCGAGGTTCACGAGAAGTACTACTCTGCCTACACTCCGAGTCGGGACTTGAAGGAGGTGAGAGACGAAGAGGGAATTCCCGAATTGGAAGACGAGTACCAACAGGAGGTGGTGAAGGAGGTGCAGAGGGACCACAAGCATGTGGTGGATGGCGGGAAGCATTCTAAGGCTGTTGTGGACGATGAGGCAGCTGAAACGCAGCCCTCCTCGCTTTATCAAGCTCGGCCGAGCGTTGAGACTGATGGTGGTGGGGTGGAGTATGAAGTCCATGTGATGGAGAAGAAAATTGTGGATGAGGAGGAGACGCGCGAGGACCGTGGCAATGGCGGAGCTGCAGCTAAAGCTCGACCGGGGTCTCGAGATGCTTTCGAAGTTGCTCGAGAGATTGAGGTTCAGTTTCAGAGAGCTTCGGAGTCGGGGAACGAAATTTCCAAGATGCTCGAGGTGGGAAAGCTTCCCCATAGTGGAAAACATGTTTTTTCCAAGACTTTAGTCTCTTCGCAACCTTCTACTAGTGCCGAGCCATCGACCTCTGCTGAAGTGGCTGCTCCTGCTCAATTGGGGTTCGATGAAGAATTGATGTTGAGGTCCAAAAATCTCTCTTCCACCTTACACAAGCTATATCTTTGGGAGAAGAAACTCTACAATGAAGTAAAGGCCGAGGAAAAGATGCGGGTGATCCATGACAGGAAGTTCAGTAAGCTAAAGCATTTAGATGAAAGGGGCGCAGAAGCTCATAAAGTTCATAAAACTCGAACTTTAATAAGGAGCCTGTCCACAAAAATTAAAATTGCAATTCAAGTGGTAGACAAGATATCTGTGACTATAAGTAAGATCAGGGATGAAGAATTATGGCCGCAACTGAACGAATTAATTCAGGGGTTAACCAGGATGTGGAAATGTATGCTCGAATGTCATAGTAGCCAGTGCCTAGCAATTAGAGAAGCCAGAGGTTTAGGTCCTATCGGATCTGGAAAAAAGCTTAATGATGGTCATCTTGAGACCACATTGCAGCTTGAACATGAGCTTATTAACTGGACTTTCAGATTCTCTACTTGGATTAGTGCGCAGAAGGGATATGTGCGAGCTTTGAACAAGTGGCTTTTAAAATGTCTTCTGTATGAACCGGAAGAAACACCGGATGGAATAGTTCCCTTTTCACCTGGTAGAATAGGTGCACCCCCAATTTTTGTAATATGTAACCAATGGTCACAGGCTTTGGAGAATATATCTGAAAGAGAGGTGGTTGAATCGATGCGTATTTTCACCACAAGTGTGCTTCAGACTTGGGAACAAGATAAGATTGAAATGCGCCAGAGGATGGTGGCAAACAAGGATCTAGAGAAACAAGTTAAGAACTTGGATAGAAAGGACCAAAAAATACAGAAAGAAATTCAGGCATTAGACAAGAAAGTTTTGCTAGCATCAGGGGATGGTGACGGACTCTCGGTTGCTGGGCAGATTGTATATCAGAGTGACACTAGAAATAGTACTTTACAGGGTAGTCTGCAACGCATTTTTGAGGCCATGGAAAGGTTCACCGATAGCTCTACAAAAGTTTATGAGGAGCTTTTGCAACGTAATGAAGAAGAAAGGCTTGCTAGAGCGGCCGAGAGGGTTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632

Protein Analysis

814

Amino Acids

90.91

Weight (kDa)

6.02

Isoelectric Point (pI)

48.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF630	PF04783	1 - 59	9.1e-27	Protein of unknown function (DUF630)
DUF632	PF04782	396 - 698	5e-108	Protein of unknown function (DUF632)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010996)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35240 AT4G35240 AT4G35240 AT4G35240
fragaria_vesca	FvH4_2g16330
malus_domestica	MD05G1131700.v1.1 MD10G1134700.v1.1
prunus_persica	Prupe.8G176200_v2.0.a1
pyrus_communis	pycom05g12840 pycom10g11620
rosa_chinensis	RchiOBHm_Chr6g0280091
rosa_laevigata	RLG00000013091
rosa_multiflora	Rmu_sc0007137.1_g000002
rosa_roxburghii	Rroxscaffold_7G00188410
rosa_rugosa	Rorug06G0129900
rosa_samantha	Rh6AG240100 Rh6BG244000 Rh6CG245600 Rh6DG236500
rosa_wichuraiana	Rw6G020930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	452, 1884
AccB7I	CCANNNNNTGG	3 cut(s)	1090, 1710, 2137
AccBSI	CCGCTC	2 cut(s)	150, 2423
AccI	GTMKAC	1 cut(s)	1623
AccII	CGCG	3 cut(s)	262, 1123, 1125
AclWI	GGATC	3 cut(s)	1489, 1792, 2160
AcoI	YGGCCR	7 cut(s)	36, 145, 228, 349, 1040, 1670, 2424
AcsI	RAATTY	8 cut(s)	277, 283, 553, 574, 694, 891, 1242, 2208
AcuI	CTGAAG	4 cut(s)	723, 777, 1374, 2081
AcyI	GRCGYC	1 cut(s)	479
AfaI	GTAC	3 cut(s)	836, 914, 2308
AfeI	AGCGCT	2 cut(s)	59, 92
AflIII	ACRYGT	2 cut(s)	522, 1288
AhlI	ACTAGT	1 cut(s)	1328
AjnI	CCWGG	2 cut(s)	1703, 1978
AleI	CACNNNNGTG	1 cut(s)	2090
AloI	GAACNNNNNNTCC	2 cut(s)	1229, 1261
Alw21I	GWGCWC	3 cut(s)	17, 724, 1996
Alw26I	GTCTC	7 cut(s)	604, 873, 1046, 1112, 1176, 1315, 1811
Alw44I	GTGCAC	1 cut(s)	1992
AlwI	GGATC	3 cut(s)	1489, 1792, 2160
AlwNI	CAGNNNCTG	1 cut(s)	596
Ama87I	CYCGRG	3 cut(s)	1173, 1194, 1256
Aor51HI	AGCGCT	2 cut(s)	59, 92
ApaLI	GTGCAC	1 cut(s)	1992
ApeKI	GCWGC	7 cut(s)	60, 1004, 1015, 1148, 1151, 1361, 1828
ApoI	RAATTY	8 cut(s)	277, 283, 553, 574, 694, 891, 1242, 2208
ArsI	GACNNNNNNTTYG	2 cut(s)	275, 307
AseI	ATTAAT	1 cut(s)	1688
Asp700I	GAANNNNTTC	1 cut(s)	1689
AspLEI	GCGC	8 cut(s)	60, 93, 264, 453, 1125, 1544, 1885, 2130
AspS9I	GGNCC	8 cut(s)	109, 291, 709, 946, 1129, 1404, 1775, 2188
AsuC2I	CCSGG	4 cut(s)	53, 193, 289, 1167
AsuHPI	GGTGA	8 cut(s)	77, 886, 940, 1504, 1968, 2074, 2264, 2347
AsuII	TTCGAA	2 cut(s)	551, 1185
AsuNHI	GCTAGC	1 cut(s)	2236
AvaI	CYCGRG	3 cut(s)	1173, 1194, 1256
AvaII	GGWCC	7 cut(s)	291, 709, 946, 1129, 1404, 1775, 2188
BaeGI	GKGCMC	1 cut(s)	1996
BanII	GRGCYC	2 cut(s)	17, 515
BarI	GAAGNNNNNNTAC	2 cut(s)	1442, 1474
BauI	CACGAG	2 cut(s)	10, 827
BbsI	GAAGAC	2 cut(s)	912, 1922
Bbv12I	GWGCWC	3 cut(s)	17, 724, 1996
BbvI	GCAGC	7 cut(s)	47, 1016, 1027, 1135, 1163, 1348, 1840
BceAI	ACGGC	3 cut(s)	23, 397, 676
BciT130I	CCWGG	2 cut(s)	1705, 1980
BcnI	CCSGG	4 cut(s)	53, 193, 289, 1167
BcoDI	GTCTC	7 cut(s)	604, 873, 1046, 1112, 1176, 1315, 1811
BcuI	ACTAGT	1 cut(s)	1328
BfaI	CTAG	6 cut(s)	1329, 1748, 2156, 2237, 2298, 2418
BfmI	CTRYAG	1 cut(s)	1149
BfoI	RGCGCY	2 cut(s)	61, 94
BmcAI	AGTACT	2 cut(s)	836, 2308
Bme1390I	CCNGG	6 cut(s)	53, 193, 289, 1167, 1705, 1980
Bme18I	GGWCC	7 cut(s)	291, 709, 946, 1129, 1404, 1775, 2188
BmeT110I	CYCGRG	3 cut(s)	1173, 1194, 1256
BmgT120I	GGNCC	8 cut(s)	109, 291, 709, 946, 1129, 1404, 1775, 2188
BmiI	GGNNCC	4 cut(s)	292, 293, 947, 1586
BmrFI	CCNGG	6 cut(s)	53, 193, 289, 1167, 1705, 1980
BmsI	GCATC	7 cut(s)	166, 502, 1168, 1242, 1476, 2061, 2249
BmtI	GCTAGC	1 cut(s)	2240
BoxI	GACNNNNGTC	2 cut(s)	1168, 1307
BpiI	GAAGAC	2 cut(s)	912, 1922
Bpu14I	TTCGAA	2 cut(s)	551, 1185
BpuEI	CTTGAG	1 cut(s)	1835
BpuMI	CCSGG	4 cut(s)	53, 193, 289, 1167
Bsa29I	ATCGAT	1 cut(s)	2069
BsaBI	GATNNNNATC	1 cut(s)	1809
BsaHI	GRCGYC	1 cut(s)	479
BsaI	GGTCTC	2 cut(s)	1176, 1811
BsaJI	CCNNGG	5 cut(s)	105, 1132, 1166, 1476, 2343
BsaWI	WCCGGW	2 cut(s)	1942, 1954
BsaXI	ACNNNNNCTCC	2 cut(s)	293, 323
Bse118I	RCCGGY	1 cut(s)	226
Bse1I	ACTGG	2 cut(s)	1741, 1856
Bse3DI	GCAATG	2 cut(s)	1144, 1823
Bse8I	GATNNNNATC	1 cut(s)	1809
BseBI	CCWGG	2 cut(s)	1705, 1980
BseCI	ATCGAT	1 cut(s)	2069
BseDI	CCNNGG	5 cut(s)	105, 1132, 1166, 1476, 2343
BseGI	GGATG	8 cut(s)	188, 970, 1114, 1663, 1713, 1963, 2142, 2254
BseJI	GATNNNNATC	1 cut(s)	1809
BseMI	GCAATG	2 cut(s)	1144, 1823
BseMII	CTCAG	1 cut(s)	148
BseNI	ACTGG	2 cut(s)	1741, 1856
BseRI	GAGGAG	6 cut(s)	419, 756, 1012, 1127, 1130, 2396
BseSI	GKGCMC	1 cut(s)	1996
BseX3I	CGGCCG	4 cut(s)	36, 145, 1040, 2424
BseXI	GCAGC	7 cut(s)	47, 1016, 1027, 1135, 1163, 1348, 1840
BseYI	CCCAGC	1 cut(s)	2270
BsgI	GTGCAG	2 cut(s)	350, 959
Bsh1236I	CGCG	3 cut(s)	262, 1123, 1125
Bsh1285I	CGRYCG	5 cut(s)	39, 148, 1043, 1166, 2427
BshVI	ATCGAT	1 cut(s)	2069
BsiEI	CGRYCG	5 cut(s)	39, 148, 1043, 1166, 2427
BsiHKAI	GWGCWC	3 cut(s)	17, 724, 1996
BsiHKCI	CYCGRG	3 cut(s)	1173, 1194, 1256
BslFI	GGGAC	3 cut(s)	277, 875, 959
BsmAI	GTCTC	7 cut(s)	604, 873, 1046, 1112, 1176, 1315, 1811
BsmBI	CGTCTC	2 cut(s)	873, 1112
BsmFI	GGGAC	3 cut(s)	277, 875, 959
BsmI	GAATGC	1 cut(s)	976
Bso31I	GGTCTC	2 cut(s)	1176, 1811
BsoBI	CYCGRG	3 cut(s)	1173, 1194, 1256
Bsp119I	TTCGAA	2 cut(s)	551, 1185
Bsp1286I	GDGCHC	4 cut(s)	17, 515, 724, 1996
Bsp143I	GATC	5 cut(s)	243, 1494, 1650, 1784, 2152
Bsp19I	CCATGG	1 cut(s)	2343
BspCNI	CTCAG	1 cut(s)	147
BspDI	ATCGAT	1 cut(s)	2069
BspFNI	CGCG	3 cut(s)	262, 1123, 1125
BspLI	GGNNCC	4 cut(s)	292, 293, 947, 1586
BspMAI	CTGCAG	1 cut(s)	1153
BspOI	GCTAGC	1 cut(s)	2240
BspPI	GGATC	3 cut(s)	1489, 1792, 2160
BspT104I	TTCGAA	2 cut(s)	551, 1185
BspTNI	GGTCTC	2 cut(s)	1176, 1811
BsrBI	CCGCTC	2 cut(s)	150, 2423
BsrDI	GCAATG	2 cut(s)	1144, 1823
BsrFI	RCCGGY	1 cut(s)	226
BsrI	ACTGG	2 cut(s)	1741, 1856
BssAI	RCCGGY	1 cut(s)	226
BssECI	CCNNGG	5 cut(s)	105, 1132, 1166, 1476, 2343
BssMI	GATC	5 cut(s)	243, 1494, 1650, 1784, 2152
BssNI	GRCGYC	1 cut(s)	479
BssSI	CACGAG	2 cut(s)	10, 827
BssT1I	CCWWGG	1 cut(s)	2343
Bst2BI	CACGAG	2 cut(s)	10, 827
Bst2UI	CCWGG	2 cut(s)	1705, 1980
Bst4CI	ACNGT	3 cut(s)	644, 726, 1133
Bst6I	CTCTTC	3 cut(s)	879, 1318, 1423
BstACI	GRCGYC	1 cut(s)	479
BstBI	TTCGAA	2 cut(s)	551, 1185
BstC8I	GCNNGC	6 cut(s)	228, 335, 413, 1902, 2238, 2415
BstDEI	CTNAG	3 cut(s)	134, 756, 981
BstDSI	CCRYGG	2 cut(s)	1132, 2343
BstENI	CCTNNNNNAGG	2 cut(s)	74, 104
BstF5I	GGATG	8 cut(s)	188, 970, 1114, 1663, 1713, 1963, 2142, 2254
BstFNI	CGCG	3 cut(s)	262, 1123, 1125
BstH2I	RGCGCY	2 cut(s)	61, 94
BstHHI	GCGC	8 cut(s)	60, 93, 264, 453, 1125, 1544, 1885, 2130
BstKTI	GATC	5 cut(s)	246, 1497, 1653, 1787, 2155
BstMAI	GTCTC	7 cut(s)	604, 873, 1046, 1112, 1176, 1315, 1811
BstMBI	GATC	5 cut(s)	243, 1494, 1650, 1784, 2152
BstMCI	CGRYCG	5 cut(s)	39, 148, 1043, 1166, 2427
BstMWI	GCNNNNNNNGC	9 cut(s)	57, 116, 778, 781, 1024, 1157, 1358, 1367, 2423
BstNI	CCWGG	2 cut(s)	1705, 1980
BstNSI	RCATGY	2 cut(s)	959, 1292
BstPAI	GACNNNNGTC	2 cut(s)	1168, 1307
BstSCI	CCNGG	6 cut(s)	51, 191, 287, 1165, 1703, 1978
BstSFI	CTRYAG	1 cut(s)	1149
BstSLI	GKGCMC	1 cut(s)	1996
BstUI	CGCG	3 cut(s)	262, 1123, 1125
BstV1I	GCAGC	7 cut(s)	47, 1016, 1027, 1135, 1163, 1348, 1840
BstV2I	GAAGAC	2 cut(s)	912, 1922
BstX2I	RGATCY	2 cut(s)	1784, 2152
BstYI	RGATCY	2 cut(s)	1784, 2152
BstZI	CGGCCG	4 cut(s)	36, 145, 1040, 2424
Bsu15I	ATCGAT	1 cut(s)	2069
BsuTUI	ATCGAT	1 cut(s)	2069
BtgI	CCRYGG	2 cut(s)	1132, 2343
BtgZI	GCGATG	1 cut(s)	100
BtsCI	GGATG	8 cut(s)	188, 970, 1114, 1663, 1713, 1963, 2142, 2254
BtsI	GCAGTG	1 cut(s)	362
BtsIMutI	CAGTG	2 cut(s)	362, 1748
Cac8I	GCNNGC	6 cut(s)	228, 335, 413, 1902, 2238, 2415
CaiI	CAGNNNCTG	1 cut(s)	596
CfoI	GCGC	8 cut(s)	60, 93, 264, 453, 1125, 1544, 1885, 2130
Cfr10I	RCCGGY	1 cut(s)	226
Cfr13I	GGNCC	8 cut(s)	109, 291, 709, 946, 1129, 1404, 1775, 2188
ClaI	ATCGAT	1 cut(s)	2069
CseI	GACGC	3 cut(s)	487, 602, 1129
CsiI	ACCWGGT	1 cut(s)	1978
Csp6I	GTAC	3 cut(s)	835, 913, 2307
CspCI	CAANNNNNGTGG	4 cut(s)	625, 660, 1599, 1634
CviAII	CATG	9 cut(s)	400, 454, 463, 956, 1085, 1289, 1499, 1838, 2344
CviQI	GTAC	3 cut(s)	835, 913, 2307
DdeI	CTNAG	3 cut(s)	134, 756, 981
DpnI	GATC	5 cut(s)	245, 1496, 1652, 1786, 2154
DpnII	GATC	5 cut(s)	243, 1494, 1650, 1784, 2152
DraI	TTTAAA	2 cut(s)	810, 1923
EaeI	YGGCCR	7 cut(s)	36, 145, 228, 349, 1040, 1670, 2424
EagI	CGGCCG	4 cut(s)	36, 145, 1040, 2424
Eam1104I	CTCTTC	3 cut(s)	879, 1318, 1423
EarI	CTCTTC	3 cut(s)	879, 1318, 1423
EciI	GGCGGA	3 cut(s)	678, 759, 1158
Ecl136II	GAGCTC	1 cut(s)	15
EclXI	CGGCCG	4 cut(s)	36, 145, 1040, 2424
Eco130I	CCWWGG	1 cut(s)	2343
Eco24I	GRGCYC	2 cut(s)	17, 515
Eco31I	GGTCTC	2 cut(s)	1176, 1811
Eco32I	GATATC	2 cut(s)	569, 1632
Eco47I	GGWCC	7 cut(s)	291, 709, 946, 1129, 1404, 1775, 2188
Eco47III	AGCGCT	2 cut(s)	59, 92
Eco52I	CGGCCG	4 cut(s)	36, 145, 1040, 2424
Eco53kI	GAGCTC	1 cut(s)	15
Eco57I	CTGAAG	4 cut(s)	723, 777, 1374, 2081
Eco88I	CYCGRG	3 cut(s)	1173, 1194, 1256
EcoICRI	GAGCTC	1 cut(s)	15
EcoNI	CCTNNNNNAGG	2 cut(s)	74, 104
EcoO109I	RGGNCCY	2 cut(s)	291, 1775
EcoRI	GAATTC	3 cut(s)	553, 694, 891
EcoRII	CCWGG	2 cut(s)	1703, 1978
EcoRV	GATATC	2 cut(s)	569, 1632
EcoT14I	CCWWGG	1 cut(s)	2343
EcoT38I	GRGCYC	2 cut(s)	17, 515
ErhI	CCWWGG	1 cut(s)	2343
Esp3I	CGTCTC	2 cut(s)	873, 1112
FaeI	CATG	9 cut(s)	403, 457, 466, 959, 1088, 1292, 1502, 1841, 2347
FalI	AAGNNNNNCTT	2 cut(s)	2397, 2429
FaqI	GGGAC	3 cut(s)	277, 875, 959
FatI	CATG	9 cut(s)	399, 453, 462, 955, 1084, 1288, 1498, 1837, 2343
FauI	CCCGC	4 cut(s)	231, 404, 962, 1482
FblI	GTMKAC	1 cut(s)	1623
FokI	GGATG	8 cut(s)	175, 977, 1121, 1670, 1720, 1970, 2149, 2261
FriOI	GRGCYC	2 cut(s)	17, 515
FspAI	RTGCGCAY	1 cut(s)	452
FspBI	CTAG	6 cut(s)	1329, 1748, 2156, 2237, 2298, 2418
FspI	TGCGCA	2 cut(s)	452, 1884
GlaI	GCGC	8 cut(s)	59, 92, 263, 452, 1124, 1543, 1884, 2129
GsaI	CCCAGC	1 cut(s)	2274
HaeII	RGCGCY	2 cut(s)	61, 94
HgaI	GACGC	3 cut(s)	487, 602, 1129
HhaI	GCGC	8 cut(s)	60, 93, 264, 453, 1125, 1544, 1885, 2130
Hin1I	GRCGYC	1 cut(s)	479
Hin1II	CATG	9 cut(s)	403, 457, 466, 959, 1088, 1292, 1502, 1841, 2347
Hin6I	GCGC	8 cut(s)	58, 91, 262, 451, 1123, 1542, 1883, 2128
HinP1I	GCGC	8 cut(s)	58, 91, 262, 451, 1123, 1542, 1883, 2128
HincII	GTYRAC	1 cut(s)	1701
HindII	GTYRAC	1 cut(s)	1701
HindIII	AAGCTT	2 cut(s)	1268, 1796
HpaI	GTTAAC	1 cut(s)	1701
HphI	GGTGA	8 cut(s)	77, 886, 940, 1504, 1968, 2074, 2264, 2347
Hpy166II	GTNNAC	7 cut(s)	826, 994, 1593, 1624, 1701, 1994, 2355
Hpy8I	GTNNAC	7 cut(s)	826, 994, 1593, 1624, 1701, 1994, 2355
Hpy99I	CGWCG	5 cut(s)	29, 77, 361, 481, 730
HpyAV	CCTTC	5 cut(s)	786, 862, 925, 1332, 1882
HpyCH4III	ACNGT	3 cut(s)	644, 726, 1133
HpyCH4IV	ACGT	2 cut(s)	522, 2395
HpyF10VI	GCNNNNNNNGC	9 cut(s)	57, 116, 778, 781, 1024, 1157, 1358, 1367, 2423
HpyF3I	CTNAG	3 cut(s)	134, 756, 981
HpySE526I	ACGT	2 cut(s)	522, 2395
Hsp92I	GRCGYC	1 cut(s)	479
Hsp92II	CATG	9 cut(s)	403, 457, 466, 959, 1088, 1292, 1502, 1841, 2347
HspAI	GCGC	8 cut(s)	58, 91, 262, 451, 1123, 1542, 1883, 2128
KflI	GGGWCCC	1 cut(s)	291
KroI	GCCGGC	1 cut(s)	226
KroNI	GCCGGC	1 cut(s)	228
KspAI	GTTAAC	1 cut(s)	1701
Kzo9I	GATC	5 cut(s)	243, 1494, 1650, 1784, 2152
LmnI	GCTCC	9 cut(s)	20, 55, 314, 342, 719, 1145, 1369, 1584, 2383
Lsp1109I	GCAGC	7 cut(s)	47, 1016, 1027, 1135, 1163, 1348, 1840
LweI	GCATC	7 cut(s)	166, 502, 1168, 1242, 1476, 2061, 2249
MabI	ACCWGGT	1 cut(s)	1978
MaeI	CTAG	6 cut(s)	1329, 1748, 2156, 2237, 2298, 2418
MaeII	ACGT	2 cut(s)	522, 2395
MaeIII	GTNAC	5 cut(s)	1636, 2015, 2025, 2252, 2291
MalI	GATC	5 cut(s)	245, 1496, 1652, 1786, 2154
MbiI	CCGCTC	2 cut(s)	150, 2423
MboI	GATC	5 cut(s)	243, 1494, 1650, 1784, 2152
MfeI	CAATTG	2 cut(s)	530, 1373
MflI	RGATCY	2 cut(s)	1784, 2152
MhlI	GDGCHC	4 cut(s)	17, 515, 724, 1996
MlyI	GAGTC	4 cut(s)	338, 865, 1238, 2253
MmeI	TCCRAC	1 cut(s)	342
MroNI	GCCGGC	1 cut(s)	226
MroXI	GAANNNNTTC	1 cut(s)	1689
MslI	CAYNNNNRTG	3 cut(s)	954, 1530, 2090
MspA1I	CMGCKG	2 cut(s)	706, 1007
MspR9I	CCNGG	6 cut(s)	53, 193, 289, 1167, 1705, 1980
MunI	CAATTG	2 cut(s)	530, 1373
Mva1269I	GAATGC	1 cut(s)	976
MvaI	CCWGG	2 cut(s)	1705, 1980
MvnI	CGCG	3 cut(s)	262, 1123, 1125
MwoI	GCNNNNNNNGC	9 cut(s)	57, 116, 778, 781, 1024, 1157, 1358, 1367, 2423
NaeI	GCCGGC	1 cut(s)	228
NciI	CCSGG	4 cut(s)	53, 193, 289, 1167
NcoI	CCATGG	1 cut(s)	2343
NdeII	GATC	5 cut(s)	243, 1494, 1650, 1784, 2152
NgoMIV	GCCGGC	1 cut(s)	226
NheI	GCTAGC	1 cut(s)	2236
NlaIII	CATG	9 cut(s)	403, 457, 466, 959, 1088, 1292, 1502, 1841, 2347
NlaIV	GGNNCC	4 cut(s)	292, 293, 947, 1586
NmeAIII	GCCGAG	5 cut(s)	130, 1018, 1068, 1360, 1501
NmuCI	GTSAC	4 cut(s)	1636, 2025, 2252, 2291
NsbI	TGCGCA	2 cut(s)	452, 1884
NspI	RCATGY	2 cut(s)	959, 1292
NspV	TTCGAA	2 cut(s)	551, 1185
OliI	CACNNNNGTG	1 cut(s)	2090
PaeR7I	CTCGAG	3 cut(s)	1173, 1194, 1256
PciI	ACATGT	1 cut(s)	1288
PcsI	WCGNNNNNNNCGW	2 cut(s)	102, 825
PctI	GAATGC	1 cut(s)	976
PdiI	GCCGGC	1 cut(s)	228
PdmI	GAANNNNTTC	1 cut(s)	1689
PfeI	GAWTC	7 cut(s)	82, 141, 314, 326, 539, 1863, 2066
PflFI	GACNNNGTC	2 cut(s)	596, 751
PflMI	CCANNNNNTGG	3 cut(s)	1090, 1710, 2137
PleI	GAGTC	4 cut(s)	338, 864, 1237, 2253
PpsI	GAGTC	4 cut(s)	338, 864, 1237, 2253
PpuMI	RGGWCCY	2 cut(s)	291, 1775
PscI	ACATGT	1 cut(s)	1288
PshAI	GACNNNNGTC	2 cut(s)	1168, 1307
PshBI	ATTAAT	1 cut(s)	1688
Psp124BI	GAGCTC	1 cut(s)	17
Psp5II	RGGWCCY	2 cut(s)	291, 1775
Psp6I	CCWGG	2 cut(s)	1703, 1978
PspFI	CCCAGC	1 cut(s)	2270
PspGI	CCWGG	2 cut(s)	1703, 1978
PspN4I	GGNNCC	4 cut(s)	292, 293, 947, 1586
PspPI	GGNCC	8 cut(s)	109, 291, 709, 946, 1129, 1404, 1775, 2188
PspPPI	RGGWCCY	2 cut(s)	291, 1775
PspXI	VCTCGAGB	1 cut(s)	1256
PstI	CTGCAG	1 cut(s)	1153
PstNI	CAGNNNCTG	1 cut(s)	596
PsuI	RGATCY	2 cut(s)	1784, 2152
PsyI	GACNNNGTC	2 cut(s)	596, 751
PvuII	CAGCTG	1 cut(s)	1007
RsaI	GTAC	3 cut(s)	836, 914, 2308
RsaNI	GTAC	3 cut(s)	835, 913, 2307
RseI	CAYNNNNRTG	3 cut(s)	954, 1530, 2090
SacI	GAGCTC	1 cut(s)	17
Sau3AI	GATC	5 cut(s)	243, 1494, 1650, 1784, 2152
Sau96I	GGNCC	8 cut(s)	109, 291, 709, 946, 1129, 1404, 1775, 2188
ScaI	AGTACT	2 cut(s)	836, 2308
SchI	GAGTC	4 cut(s)	338, 865, 1238, 2253
ScrFI	CCNGG	6 cut(s)	53, 193, 289, 1167, 1705, 1980
SduI	GDGCHC	4 cut(s)	17, 515, 724, 1996
SexAI	ACCWGGT	1 cut(s)	1978
SfaNI	GCATC	7 cut(s)	166, 502, 1168, 1242, 1476, 2061, 2249
SfcI	CTRYAG	1 cut(s)	1149
Sfr274I	CTCGAG	3 cut(s)	1173, 1194, 1256
SfuI	TTCGAA	2 cut(s)	551, 1185
SinI	GGWCC	7 cut(s)	291, 709, 946, 1129, 1404, 1775, 2188
SlaI	CTCGAG	3 cut(s)	1173, 1194, 1256
SmiMI	CAYNNNNRTG	3 cut(s)	954, 1530, 2090
SmlI	CTYRAG	4 cut(s)	1173, 1194, 1256, 1814
SmoI	CTYRAG	4 cut(s)	1173, 1194, 1256, 1814
SpeI	ACTAGT	1 cut(s)	1328
SspI	AATATT	1 cut(s)	583
SspMI	CTAG	6 cut(s)	1329, 1748, 2156, 2237, 2298, 2418
SstI	GAGCTC	1 cut(s)	17
StyD4I	CCNGG	6 cut(s)	51, 191, 287, 1165, 1703, 1978
StyI	CCWWGG	1 cut(s)	2343
TaaI	ACNGT	3 cut(s)	644, 726, 1133
TaiI	ACGT	2 cut(s)	525, 2398
TatI	WGTACW	2 cut(s)	834, 2306
TauI	GCSGC	9 cut(s)	66, 150, 202, 259, 262, 775, 784, 1675, 2426
TfiI	GAWTC	7 cut(s)	82, 141, 314, 326, 539, 1863, 2066
TscAI	CASTG	2 cut(s)	369, 1748
TseFI	GTSAC	4 cut(s)	1636, 2025, 2252, 2291
TseI	GCWGC	7 cut(s)	60, 1004, 1015, 1148, 1151, 1361, 1828
Tsp45I	GTSAC	4 cut(s)	1636, 2025, 2252, 2291
TspGWI	ACGGA	1 cut(s)	2271
TspRI	CASTG	2 cut(s)	369, 1748
Tth111I	GACNNNGTC	2 cut(s)	596, 751
Van91I	CCANNNNNTGG	3 cut(s)	1090, 1710, 2137
VneI	GTGCAC	1 cut(s)	1992
VpaK11BI	GGWCC	7 cut(s)	291, 709, 946, 1129, 1404, 1775, 2188
VspI	ATTAAT	1 cut(s)	1688
XagI	CCTNNNNNAGG	2 cut(s)	74, 104
XapI	RAATTY	8 cut(s)	277, 283, 553, 574, 694, 891, 1242, 2208
XbaI	TCTAGA	1 cut(s)	2155
XceI	RCATGY	2 cut(s)	959, 1292
XhoI	CTCGAG	3 cut(s)	1173, 1194, 1256
XmiI	GTMKAC	1 cut(s)	1623
XmnI	GAANNNNTTC	1 cut(s)	1689
XspI	CTAG	6 cut(s)	1329, 1748, 2156, 2237, 2298, 2418
ZrmI	AGTACT	2 cut(s)	836, 2308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.