pycom05g12840

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010996
Physical Location & Seq
Reverse (-)
16366944 .. 16371224
4281 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12840.2

Sequence Viewer

Length: 2448 bp
ATGGGTTGCACGAGCTCCAAGCCCGACGAACTTCCGGCCGTTGCCTTATGCCGGGAGCGCTGCGGCTTCCTCGACGAGGCGATTCACCAGCGCTACGCCCTCGCCGAGGCCCACATCGCTTATATTAGCTCCCTCAGAGAAATCGGCCGCTCTCTGCATCAATTTATCGAGCAGGAGGTGGGCAATTCATCCGGGTCGCCGTCCTCACCCCACCTCAACCTCCCGCCGGCCAGAAAAGGCGAGACCAAATCCTCGCCGCTGCGCCACTCTCACTCAAATTCAAATTCCGGGTCTCATCTCCACTTTCACTCTTCCGACGATGATTCCGGCTCGCTCCACCACTCCGGCCACTCGTCGCCACTGCACGATACTCACTACAGCGGTCATATTGATTACATGGAGGACGGCGGGCAGGAGGGTTTCAATTCGTACCCAGATGGGTTTATGCGCATGAATTACATGAAGAACAAAGCGACGCCATCTGTGGTGCATCGGCAGAAACCCGTGAGCCCCGAAAACGTGTTTCAATTGGGGGAATCTTCTTCTTCGAATTCGTATGATGGATATCAAAATTCAAGTAGTTATCCAGACGCTGTCTCTAATCCTTATGCCGATTACGGCGGTGGGTTTAACAATTACGGTGGTGCGTATAATTACGGCGGAGGGAGTAGTGGGTATACTAATTACGGTAATTCTTCTCCGCCGGTCCGATATGGAGCACCGTCGCCACAGGCTTCGGCTTCGACCTCGTCTAAGCCACCTCCTCCGCCACCTTCGCCGCCAAGAGCTTCAGCTTGGGATTTTTTAAACCCATTCGAGGTTCAGGAAAAGTACTACTCTGCCTACACTCCGAGTCGGGACTTGAAGGAGGTGAGAGACGAAGAGGGAATTCCCGAATTGGAAGACGTGGAGTACCAGCAGGAGGTGGTGAAGGAGGTGCAGAGGGACCACAAGCATGTGGTGGATGGCGGGAAGCATTCTAAGGCTGTTGTGGACGATGAGGCAGCTGAAACGCAACCCTCCTCGGTTTATCAAGCTCGGCCGAGCGTTGAGACTGATGGTGGTGGGGTGGAGTATGAAGTCCATGTGATGGAGAAGAAGATTGTGGATGAGGAGGAGACGCGCGAGGACCGTGGCAATGGCGGAGCTGCAGCTAAAGCTCGACCGGGGTCTCGAGATGCTTTCGAAGTTGCTCGAGAGATTGAGATTCAGTTTCAGAGAGCTTCGGAGTCGGGGAAAGAAATTTCCAAGATGCTCGAGGTGGGAAAGCTTCCCCATGGTGGAAAACATGTTTCTTCCAAGACTTTAGTCTCTTCGCAACCTTCTACTAGTGCCGAGCCATCGACCTCTGCTCAAGTGGCTGCTCCTGCTCAATTGGGGTTCGATGAAGAATTGATGTTGAGGTCCAAAAATCTCTCTTCCACCTTACACAAGCTATATCTTTGGGAGAAGAAACTCTACAATGAAGTAAAGGCGGAGGAAAAGATGCGGGTGATCGATCCCCATGACAGGAAGGTCAGTAAGCTAAAGCATTTAGATGAAAGGGGCGCAGAAGCTCATAAAGTTGATAAAACTCGAACTTTAATAAGGAGTCTGTCCACAAAAATTAAAATTGCAATTCAAGTGGTAGACAAGATATCTGTGACTATAAGTAAGATCAGGGATGAAGAATTATGGCCACAACTGAACGAATTAATTCAGGGGTTAACCAGGATGTGGAAATGTATGCTCGAATGTCATAGTAGCCAGTGCCTAGCAGTTAGAGAAGCCAGAGGTTTAGGTCCTATTGGATCTGGAAAAAAGCTTAATGATGGTCATCTTGATGCCACATTGCAGCTTGAACATCAGCTTATTAACTGGACTTTCAGATTCTCTACTTGGATTAGTGCGCAGAAGGGATATGTGCGAGCTTTGAACAAGTGGCTTTTAAAATGTCTTCTGTATGAACCGGAAGAAACACCAGATGGAATAGTTCCCTTTTCACCCGGTAGAATAGGTGCACCCCCAATTTTTGTAATATGTAACCAGTGGTCACAGGCTTTGGAAAATATATCTGAAAGAGAGGTGGTTGAGTCGATGCGTATTTTCACCACAAGTGTGCTTCAGATTTGGGAACAAGATAAGATTGAAATGCGCCAGTGGATGGTGGCACACAAGGATCAAGAGAAACAAGTTAAGAAATTGGATAGAAAGGACCAAAAAATACAGAAAGAAATTCAGGCATTAGACAAGAAAATTGTGCTAGCATCAGGGGATGGTGACGGACTCTCGGTTGCTGGGCAGATTGTATATCAGAGTGACACTAGAAATAGTACTTTACAGGGTAGTCTGCAACGCATTTTTGAGGCCATGGAAAGGTTCACCGATAGCTCCACAAAAGTTTATGAGGAGCTTTTGCAACGTAATGAAGAAGAAAGGCTTGCTAGAGCAGCCGAGAGGGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

816

Amino Acids

90.93

Weight (kDa)

6.0

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35240 AT4G35240 AT4G35240 AT4G35240
fragaria_vesca FvH4_2g16330
malus_domestica MD05G1131700.v1.1 MD10G1134700.v1.1
prunus_persica Prupe.8G176200_v2.0.a1
pyrus_communis pycom05g12840 pycom10g11620
rosa_chinensis RchiOBHm_Chr6g0280091
rosa_laevigata RLG00000013091
rosa_multiflora Rmu_sc0007137.1_g000002
rosa_roxburghii Rroxscaffold_7G00188410
rosa_rugosa Rorug06G0129900
rosa_samantha Rh6AG240100 Rh6BG244000 Rh6CG245600 Rh6DG236500
rosa_wichuraiana Rw6G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1514
Acc16I TGCGCA 2 cut(s) 449, 1890
AccB7I CCANNNNNTGG 3 cut(s) 1090, 1716, 2143
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 2 cut(s) 677, 1629
AccII CGCG 2 cut(s) 1123, 1125
AclWI GGATC 3 cut(s) 1493, 1798, 2166
AcoI YGGCCR 6 cut(s) 36, 145, 228, 346, 1040, 1676
AcsI RAATTY 7 cut(s) 277, 283, 550, 571, 888, 1242, 2214
AcuI CTGAAG 2 cut(s) 774, 2087
AcyI GRCGYC 1 cut(s) 476
AfaI GTAC 4 cut(s) 431, 833, 914, 2314
AfeI AGCGCT 2 cut(s) 59, 92
AflIII ACRYGT 2 cut(s) 519, 1288
AgsI TTSAA 9 cut(s) 282, 424, 527, 576, 865, 1622, 1841, 1915, 2129
AhlI ACTAGT 1 cut(s) 1328
AjiI CACGTC 1 cut(s) 907
AjnI CCWGG 1 cut(s) 1709
AleI CACNNNNGTG 1 cut(s) 2096
Alw21I GWGCWC 3 cut(s) 17, 721, 2002
Alw26I GTCTC 8 cut(s) 236, 297, 601, 870, 1046, 1112, 1176, 1315
Alw44I GTGCAC 1 cut(s) 1998
AlwI GGATC 3 cut(s) 1493, 1798, 2166
AlwNI CAGNNNCTG 1 cut(s) 593
Ama87I CYCGRG 3 cut(s) 1173, 1194, 1256
Aor51HI AGCGCT 2 cut(s) 59, 92
AoxI GGCC 8 cut(s) 36, 108, 145, 228, 346, 1040, 1676, 2346
ApaLI GTGCAC 1 cut(s) 1998
ApeKI GCWGC 8 cut(s) 60, 259, 1004, 1148, 1151, 1361, 1834, 2429
ApoI RAATTY 7 cut(s) 277, 283, 550, 571, 888, 1242, 2214
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AseI ATTAAT 1 cut(s) 1694
Asp700I GAANNNNTTC 1 cut(s) 1695
AspLEI GCGC 8 cut(s) 60, 93, 264, 450, 1125, 1550, 1891, 2136
AspS9I GGNCC 7 cut(s) 109, 706, 946, 1129, 1404, 1781, 2194
AsuC2I CCSGG 5 cut(s) 53, 193, 289, 1167, 1986
AsuHPI GGTGA 9 cut(s) 77, 198, 883, 940, 1504, 1974, 2080, 2270, 2353
AsuII TTCGAA 2 cut(s) 548, 1185
AsuNHI GCTAGC 1 cut(s) 2242
AvaI CYCGRG 3 cut(s) 1173, 1194, 1256
AvaII GGWCC 6 cut(s) 706, 946, 1129, 1404, 1781, 2194
BaeGI GKGCMC 1 cut(s) 2002
BalI TGGCCA 1 cut(s) 1678
BanII GRGCYC 2 cut(s) 17, 512
BarI GAAGNNNNNNTAC 2 cut(s) 1442, 1474
BauI CACGAG 1 cut(s) 10
BbsI GAAGAC 2 cut(s) 909, 1928
Bbv12I GWGCWC 3 cut(s) 17, 721, 2002
BbvI GCAGC 8 cut(s) 47, 246, 1016, 1135, 1163, 1348, 1846, 2441
BceAI ACGGC 5 cut(s) 23, 184, 421, 634, 673
BciT130I CCWGG 1 cut(s) 1711
BcnI CCSGG 5 cut(s) 53, 193, 289, 1167, 1986
BcoDI GTCTC 8 cut(s) 236, 297, 601, 870, 1046, 1112, 1176, 1315
BcuI ACTAGT 1 cut(s) 1328
BfaI CTAG 5 cut(s) 1329, 1754, 2243, 2304, 2424
BfmI CTRYAG 2 cut(s) 376, 1149
BfoI RGCGCY 2 cut(s) 61, 94
BmcAI AGTACT 2 cut(s) 833, 2314
Bme1390I CCNGG 6 cut(s) 53, 193, 289, 1167, 1711, 1986
Bme18I GGWCC 6 cut(s) 706, 946, 1129, 1404, 1781, 2194
BmeT110I CYCGRG 3 cut(s) 1173, 1194, 1256
BmgBI CACGTC 1 cut(s) 907
BmgT120I GGNCC 7 cut(s) 109, 706, 946, 1129, 1404, 1781, 2194
BmiI GGNNCC 1 cut(s) 947
BmrFI CCNGG 6 cut(s) 53, 193, 289, 1167, 1711, 1986
BmsI GCATC 8 cut(s) 166, 499, 1168, 1242, 1476, 1813, 2067, 2255
BmtI GCTAGC 1 cut(s) 2246
BoxI GACNNNNGTC 2 cut(s) 1168, 1307
BpiI GAAGAC 2 cut(s) 909, 1928
Bpu14I TTCGAA 2 cut(s) 548, 1185
BpuEI CTTGAG 1 cut(s) 1338
BpuMI CCSGG 5 cut(s) 53, 193, 289, 1167, 1986
Bsa29I ATCGAT 1 cut(s) 1497
BsaBI GATNNNNATC 2 cut(s) 564, 1815
BsaHI GRCGYC 1 cut(s) 476
BsaI GGTCTC 3 cut(s) 236, 297, 1176
BsaJI CCNNGG 6 cut(s) 105, 1023, 1132, 1166, 1276, 2349
BsaWI WCCGGW 1 cut(s) 1948
Bse118I RCCGGY 2 cut(s) 226, 703
Bse1I ACTGG 4 cut(s) 1747, 1862, 2026, 2137
Bse3DI GCAATG 2 cut(s) 1144, 1829
Bse8I GATNNNNATC 2 cut(s) 564, 1815
BseBI CCWGG 1 cut(s) 1711
BseCI ATCGAT 1 cut(s) 1497
BseDI CCNNGG 6 cut(s) 105, 1023, 1132, 1166, 1276, 2349
BseGI GGATG 7 cut(s) 188, 970, 1114, 1669, 1719, 2148, 2260
BseJI GATNNNNATC 2 cut(s) 564, 1815
BseMI GCAATG 2 cut(s) 1144, 1829
BseMII CTCAG 1 cut(s) 148
BseNI ACTGG 4 cut(s) 1747, 1862, 2026, 2137
BseRI GAGGAG 5 cut(s) 753, 1012, 1127, 1130, 2402
BseSI GKGCMC 1 cut(s) 2002
BseX3I CGGCCG 3 cut(s) 36, 145, 1040
BseXI GCAGC 8 cut(s) 47, 246, 1016, 1135, 1163, 1348, 1846, 2441
BseYI CCCAGC 1 cut(s) 2276
BsgI GTGCAG 2 cut(s) 347, 959
Bsh1236I CGCG 2 cut(s) 1123, 1125
Bsh1285I CGRYCG 4 cut(s) 39, 148, 1043, 1166
BshFI GGCC 8 cut(s) 38, 110, 147, 230, 348, 1042, 1678, 2348
BshVI ATCGAT 1 cut(s) 1497
BsiEI CGRYCG 4 cut(s) 39, 148, 1043, 1166
BsiHKAI GWGCWC 3 cut(s) 17, 721, 2002
BsiHKCI CYCGRG 3 cut(s) 1173, 1194, 1256
BslFI GGGAC 2 cut(s) 872, 959
BsmAI GTCTC 8 cut(s) 236, 297, 601, 870, 1046, 1112, 1176, 1315
BsmBI CGTCTC 2 cut(s) 870, 1112
BsmFI GGGAC 2 cut(s) 872, 959
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 8 cut(s) 38, 110, 147, 230, 348, 1042, 1678, 2348
Bso31I GGTCTC 3 cut(s) 236, 297, 1176
BsoBI CYCGRG 3 cut(s) 1173, 1194, 1256
Bsp119I TTCGAA 2 cut(s) 548, 1185
Bsp1286I GDGCHC 4 cut(s) 17, 512, 721, 2002
Bsp143I GATC 5 cut(s) 1494, 1498, 1656, 1790, 2158
Bsp19I CCATGG 2 cut(s) 1276, 2349
BspANI GGCC 8 cut(s) 38, 110, 147, 230, 348, 1042, 1678, 2348
BspCNI CTCAG 1 cut(s) 147
BspDI ATCGAT 1 cut(s) 1497
BspFNI CGCG 2 cut(s) 1123, 1125
BspLI GGNNCC 1 cut(s) 947
BspMAI CTGCAG 1 cut(s) 1153
BspOI GCTAGC 1 cut(s) 2246
BspPI GGATC 3 cut(s) 1493, 1798, 2166
BspT104I TTCGAA 2 cut(s) 548, 1185
BspTNI GGTCTC 3 cut(s) 236, 297, 1176
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 2 cut(s) 1144, 1829
BsrFI RCCGGY 2 cut(s) 226, 703
BsrI ACTGG 4 cut(s) 1747, 1862, 2026, 2137
BssAI RCCGGY 2 cut(s) 226, 703
BssECI CCNNGG 6 cut(s) 105, 1023, 1132, 1166, 1276, 2349
BssMI GATC 5 cut(s) 1494, 1498, 1656, 1790, 2158
BssNAI GTATAC 1 cut(s) 678
BssNI GRCGYC 1 cut(s) 476
BssSI CACGAG 1 cut(s) 10
BssT1I CCWWGG 2 cut(s) 1276, 2349
Bst1107I GTATAC 1 cut(s) 678
Bst2BI CACGAG 1 cut(s) 10
Bst2UI CCWGG 1 cut(s) 1711
Bst4CI ACNGT 4 cut(s) 641, 689, 723, 1133
Bst6I CTCTTC 4 cut(s) 316, 876, 1318, 1423
BstACI GRCGYC 1 cut(s) 476
BstBI TTCGAA 2 cut(s) 548, 1185
BstC8I GCNNGC 6 cut(s) 228, 332, 410, 1908, 2244, 2421
BstDEI CTNAG 3 cut(s) 134, 753, 981
BstDSI CCRYGG 3 cut(s) 1132, 1276, 2349
BstENI CCTNNNNNAGG 2 cut(s) 74, 104
BstF5I GGATG 7 cut(s) 188, 970, 1114, 1669, 1719, 2148, 2260
BstFNI CGCG 2 cut(s) 1123, 1125
BstH2I RGCGCY 2 cut(s) 61, 94
BstHHI GCGC 8 cut(s) 60, 93, 264, 450, 1125, 1550, 1891, 2136
BstKTI GATC 5 cut(s) 1497, 1501, 1659, 1793, 2161
BstMAI GTCTC 8 cut(s) 236, 297, 601, 870, 1046, 1112, 1176, 1315
BstMBI GATC 5 cut(s) 1494, 1498, 1656, 1790, 2158
BstMCI CGRYCG 4 cut(s) 39, 148, 1043, 1166
BstMWI GCNNNNNNNGC 7 cut(s) 57, 116, 775, 1157, 1358, 1367, 2429
BstNI CCWGG 1 cut(s) 1711
BstNSI RCATGY 2 cut(s) 959, 1292
BstPAI GACNNNNGTC 2 cut(s) 1168, 1307
BstSCI CCNGG 6 cut(s) 51, 191, 287, 1165, 1709, 1984
BstSFI CTRYAG 2 cut(s) 376, 1149
BstSLI GKGCMC 1 cut(s) 2002
BstUI CGCG 2 cut(s) 1123, 1125
BstV1I GCAGC 8 cut(s) 47, 246, 1016, 1135, 1163, 1348, 1846, 2441
BstV2I GAAGAC 2 cut(s) 909, 1928
BstX2I RGATCY 1 cut(s) 1790
BstYI RGATCY 1 cut(s) 1790
BstZ17I GTATAC 1 cut(s) 678
BstZI CGGCCG 3 cut(s) 36, 145, 1040
Bsu15I ATCGAT 1 cut(s) 1497
BsuRI GGCC 8 cut(s) 38, 110, 147, 230, 348, 1042, 1678, 2348
BsuTUI ATCGAT 1 cut(s) 1497
BtgI CCRYGG 3 cut(s) 1132, 1276, 2349
BtgZI GCGATG 1 cut(s) 100
BtrI CACGTC 1 cut(s) 907
BtsCI GGATG 7 cut(s) 188, 970, 1114, 1669, 1719, 2148, 2260
BtsI GCAGTG 1 cut(s) 359
BtsIMutI CAGTG 4 cut(s) 359, 1754, 2033, 2144
Cac8I GCNNGC 6 cut(s) 228, 332, 410, 1908, 2244, 2421
CaiI CAGNNNCTG 1 cut(s) 593
CfoI GCGC 8 cut(s) 60, 93, 264, 450, 1125, 1550, 1891, 2136
Cfr10I RCCGGY 2 cut(s) 226, 703
Cfr13I GGNCC 7 cut(s) 109, 706, 946, 1129, 1404, 1781, 2194
ClaI ATCGAT 1 cut(s) 1497
CpoI CGGWCCG 1 cut(s) 706
CseI GACGC 3 cut(s) 484, 599, 1129
Csp6I GTAC 4 cut(s) 430, 832, 913, 2313
CspCI CAANNNNNGTGG 4 cut(s) 622, 657, 1605, 1640
CspI CGGWCCG 1 cut(s) 706
CviAII CATG 9 cut(s) 397, 451, 460, 956, 1085, 1277, 1289, 1505, 2350
CviQI GTAC 4 cut(s) 430, 832, 913, 2313
DdeI CTNAG 3 cut(s) 134, 753, 981
DpnI GATC 5 cut(s) 1496, 1500, 1658, 1792, 2160
DpnII GATC 5 cut(s) 1494, 1498, 1656, 1790, 2158
DraI TTTAAA 2 cut(s) 807, 1929
DrdI GACNNNNNNGTC 1 cut(s) 1514
DseDI GACNNNNNNGTC 1 cut(s) 1514
EaeI YGGCCR 6 cut(s) 36, 145, 228, 346, 1040, 1676
EagI CGGCCG 3 cut(s) 36, 145, 1040
Eam1104I CTCTTC 4 cut(s) 316, 876, 1318, 1423
EarI CTCTTC 4 cut(s) 316, 876, 1318, 1423
EciI GGCGGA 5 cut(s) 675, 690, 756, 1158, 1490
Ecl136II GAGCTC 1 cut(s) 15
EclXI CGGCCG 3 cut(s) 36, 145, 1040
Eco130I CCWWGG 2 cut(s) 1276, 2349
Eco24I GRGCYC 2 cut(s) 17, 512
Eco31I GGTCTC 3 cut(s) 236, 297, 1176
Eco32I GATATC 2 cut(s) 566, 1638
Eco47I GGWCC 6 cut(s) 706, 946, 1129, 1404, 1781, 2194
Eco47III AGCGCT 2 cut(s) 59, 92
Eco52I CGGCCG 3 cut(s) 36, 145, 1040
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 2 cut(s) 774, 2087
Eco88I CYCGRG 3 cut(s) 1173, 1194, 1256
EcoICRI GAGCTC 1 cut(s) 15
EcoNI CCTNNNNNAGG 2 cut(s) 74, 104
EcoO109I RGGNCCY 1 cut(s) 1781
EcoRI GAATTC 2 cut(s) 550, 888
EcoRII CCWGG 1 cut(s) 1709
EcoRV GATATC 2 cut(s) 566, 1638
EcoT14I CCWWGG 2 cut(s) 1276, 2349
EcoT38I GRGCYC 2 cut(s) 17, 512
ErhI CCWWGG 2 cut(s) 1276, 2349
Esp3I CGTCTC 2 cut(s) 870, 1112
FaeI CATG 9 cut(s) 400, 454, 463, 959, 1088, 1280, 1292, 1508, 2353
FalI AAGNNNNNCTT 2 cut(s) 2403, 2435
FaqI GGGAC 2 cut(s) 872, 959
FatI CATG 9 cut(s) 396, 450, 459, 955, 1084, 1276, 1288, 1504, 2349
FauI CCCGC 4 cut(s) 231, 401, 962, 1482
FblI GTMKAC 2 cut(s) 677, 1629
FokI GGATG 7 cut(s) 175, 977, 1121, 1676, 1726, 2155, 2267
FriOI GRGCYC 2 cut(s) 17, 512
FspAI RTGCGCAY 1 cut(s) 449
FspBI CTAG 5 cut(s) 1329, 1754, 2243, 2304, 2424
FspI TGCGCA 2 cut(s) 449, 1890
GlaI GCGC 8 cut(s) 59, 92, 263, 449, 1124, 1549, 1890, 2135
GsaI CCCAGC 1 cut(s) 2280
HaeII RGCGCY 2 cut(s) 61, 94
HaeIII GGCC 8 cut(s) 38, 110, 147, 230, 348, 1042, 1678, 2348
HgaI GACGC 3 cut(s) 484, 599, 1129
HhaI GCGC 8 cut(s) 60, 93, 264, 450, 1125, 1550, 1891, 2136
Hin1I GRCGYC 1 cut(s) 476
Hin1II CATG 9 cut(s) 400, 454, 463, 959, 1088, 1280, 1292, 1508, 2353
Hin6I GCGC 8 cut(s) 58, 91, 262, 448, 1123, 1548, 1889, 2134
HinP1I GCGC 8 cut(s) 58, 91, 262, 448, 1123, 1548, 1889, 2134
HincII GTYRAC 1 cut(s) 1707
HindII GTYRAC 1 cut(s) 1707
HindIII AAGCTT 2 cut(s) 1268, 1802
HpaI GTTAAC 1 cut(s) 1707
HphI GGTGA 9 cut(s) 77, 198, 883, 940, 1504, 1974, 2080, 2270, 2353
Hpy166II GTNNAC 7 cut(s) 678, 994, 1599, 1630, 1707, 2000, 2361
Hpy8I GTNNAC 7 cut(s) 678, 994, 1599, 1630, 1707, 2000, 2361
Hpy99I CGWCG 6 cut(s) 29, 77, 320, 358, 478, 727
HpyAV CCTTC 6 cut(s) 783, 859, 925, 1332, 1507, 1888
HpyCH4III ACNGT 4 cut(s) 641, 689, 723, 1133
HpyCH4IV ACGT 3 cut(s) 519, 906, 2401
HpyF10VI GCNNNNNNNGC 7 cut(s) 57, 116, 775, 1157, 1358, 1367, 2429
HpyF3I CTNAG 3 cut(s) 134, 753, 981
HpySE526I ACGT 3 cut(s) 519, 906, 2401
Hsp92I GRCGYC 1 cut(s) 476
Hsp92II CATG 9 cut(s) 400, 454, 463, 959, 1088, 1280, 1292, 1508, 2353
HspAI GCGC 8 cut(s) 58, 91, 262, 448, 1123, 1548, 1889, 2134
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
KspAI GTTAAC 1 cut(s) 1707
Kzo9I GATC 5 cut(s) 1494, 1498, 1656, 1790, 2158
LmnI GCTCC 9 cut(s) 20, 55, 134, 339, 716, 1145, 1369, 2375, 2389
Lsp1109I GCAGC 8 cut(s) 47, 246, 1016, 1135, 1163, 1348, 1846, 2441
LweI GCATC 8 cut(s) 166, 499, 1168, 1242, 1476, 1813, 2067, 2255
MaeI CTAG 5 cut(s) 1329, 1754, 2243, 2304, 2424
MaeII ACGT 3 cut(s) 519, 906, 2401
MaeIII GTNAC 5 cut(s) 1642, 2021, 2031, 2258, 2297
MalI GATC 5 cut(s) 1496, 1500, 1658, 1792, 2160
MbiI CCGCTC 1 cut(s) 150
MboI GATC 5 cut(s) 1494, 1498, 1656, 1790, 2158
MfeI CAATTG 2 cut(s) 527, 1373
MflI RGATCY 1 cut(s) 1790
MhlI GDGCHC 4 cut(s) 17, 512, 721, 2002
MlsI TGGCCA 1 cut(s) 1678
MluNI TGGCCA 1 cut(s) 1678
MlyI GAGTC 5 cut(s) 862, 1238, 1600, 2081, 2259
MmeI TCCRAC 1 cut(s) 339
Mox20I TGGCCA 1 cut(s) 1678
MroNI GCCGGC 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 1695
MscI TGGCCA 1 cut(s) 1678
MslI CAYNNNNRTG 4 cut(s) 954, 1536, 1821, 2096
Msp20I TGGCCA 1 cut(s) 1678
MspA1I CMGCKG 3 cut(s) 259, 381, 1007
MspR9I CCNGG 6 cut(s) 53, 193, 289, 1167, 1711, 1986
MunI CAATTG 2 cut(s) 527, 1373
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 1 cut(s) 1711
MvnI CGCG 2 cut(s) 1123, 1125
MwoI GCNNNNNNNGC 7 cut(s) 57, 116, 775, 1157, 1358, 1367, 2429
NaeI GCCGGC 1 cut(s) 228
NciI CCSGG 5 cut(s) 53, 193, 289, 1167, 1986
NcoI CCATGG 2 cut(s) 1276, 2349
NdeII GATC 5 cut(s) 1494, 1498, 1656, 1790, 2158
NgoMIV GCCGGC 1 cut(s) 226
NheI GCTAGC 1 cut(s) 2242
NlaIII CATG 9 cut(s) 400, 454, 463, 959, 1088, 1280, 1292, 1508, 2353
NlaIV GGNNCC 1 cut(s) 947
NmeAIII GCCGAG 4 cut(s) 130, 1018, 1068, 1360
NmuCI GTSAC 4 cut(s) 1642, 2031, 2258, 2297
NsbI TGCGCA 2 cut(s) 449, 1890
NspI RCATGY 2 cut(s) 959, 1292
NspV TTCGAA 2 cut(s) 548, 1185
OliI CACNNNNGTG 1 cut(s) 2096
PaeR7I CTCGAG 3 cut(s) 1173, 1194, 1256
PciI ACATGT 1 cut(s) 1288
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 976
PdiI GCCGGC 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 1695
PfeI GAWTC 5 cut(s) 82, 323, 536, 1207, 1869
PflFI GACNNNGTC 2 cut(s) 593, 748
PflMI CCANNNNNTGG 3 cut(s) 1090, 1716, 2143
PleI GAGTC 5 cut(s) 861, 1237, 1599, 2080, 2259
PpsI GAGTC 5 cut(s) 861, 1237, 1599, 2080, 2259
PpuMI RGGWCCY 1 cut(s) 1781
PscI ACATGT 1 cut(s) 1288
PshAI GACNNNNGTC 2 cut(s) 1168, 1307
PshBI ATTAAT 1 cut(s) 1694
Psp124BI GAGCTC 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 1781
Psp6I CCWGG 1 cut(s) 1709
PspFI CCCAGC 1 cut(s) 2276
PspGI CCWGG 1 cut(s) 1709
PspN4I GGNNCC 1 cut(s) 947
PspPI GGNCC 7 cut(s) 109, 706, 946, 1129, 1404, 1781, 2194
PspPPI RGGWCCY 1 cut(s) 1781
PspXI VCTCGAGB 1 cut(s) 1256
PstI CTGCAG 1 cut(s) 1153
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 1790
PsyI GACNNNGTC 2 cut(s) 593, 748
PvuII CAGCTG 1 cut(s) 1007
RsaI GTAC 4 cut(s) 431, 833, 914, 2314
RsaNI GTAC 4 cut(s) 430, 832, 913, 2313
RseI CAYNNNNRTG 4 cut(s) 954, 1536, 1821, 2096
Rsr2I CGGWCCG 1 cut(s) 706
RsrII CGGWCCG 1 cut(s) 706
SacI GAGCTC 1 cut(s) 17
Sau3AI GATC 5 cut(s) 1494, 1498, 1656, 1790, 2158
Sau96I GGNCC 7 cut(s) 109, 706, 946, 1129, 1404, 1781, 2194
ScaI AGTACT 2 cut(s) 833, 2314
SchI GAGTC 5 cut(s) 862, 1238, 1600, 2081, 2259
ScrFI CCNGG 6 cut(s) 53, 193, 289, 1167, 1711, 1986
SduI GDGCHC 4 cut(s) 17, 512, 721, 2002
SfaNI GCATC 8 cut(s) 166, 499, 1168, 1242, 1476, 1813, 2067, 2255
SfcI CTRYAG 2 cut(s) 376, 1149
Sfr274I CTCGAG 3 cut(s) 1173, 1194, 1256
SfuI TTCGAA 2 cut(s) 548, 1185
SinI GGWCC 6 cut(s) 706, 946, 1129, 1404, 1781, 2194
SlaI CTCGAG 3 cut(s) 1173, 1194, 1256
SmiMI CAYNNNNRTG 4 cut(s) 954, 1536, 1821, 2096
SmlI CTYRAG 4 cut(s) 1173, 1194, 1256, 1353
SmoI CTYRAG 4 cut(s) 1173, 1194, 1256, 1353
SpeI ACTAGT 1 cut(s) 1328
SspMI CTAG 5 cut(s) 1329, 1754, 2243, 2304, 2424
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 6 cut(s) 51, 191, 287, 1165, 1709, 1984
StyI CCWWGG 2 cut(s) 1276, 2349
TaaI ACNGT 4 cut(s) 641, 689, 723, 1133
TaiI ACGT 3 cut(s) 522, 909, 2404
TatI WGTACW 2 cut(s) 831, 2312
TauI GCSGC 4 cut(s) 66, 150, 259, 781
TfiI GAWTC 5 cut(s) 82, 323, 536, 1207, 1869
TscAI CASTG 4 cut(s) 366, 1754, 2033, 2144
TseFI GTSAC 4 cut(s) 1642, 2031, 2258, 2297
TseI GCWGC 8 cut(s) 60, 259, 1004, 1148, 1151, 1361, 1834, 2429
Tsp45I GTSAC 4 cut(s) 1642, 2031, 2258, 2297
TspGWI ACGGA 1 cut(s) 2277
TspRI CASTG 4 cut(s) 366, 1754, 2033, 2144
Tth111I GACNNNGTC 2 cut(s) 593, 748
Van91I CCANNNNNTGG 3 cut(s) 1090, 1716, 2143
VneI GTGCAC 1 cut(s) 1998
VpaK11BI GGWCC 6 cut(s) 706, 946, 1129, 1404, 1781, 2194
VspI ATTAAT 1 cut(s) 1694
XagI CCTNNNNNAGG 2 cut(s) 74, 104
XapI RAATTY 7 cut(s) 277, 283, 550, 571, 888, 1242, 2214
XceI RCATGY 2 cut(s) 959, 1292
XhoI CTCGAG 3 cut(s) 1173, 1194, 1256
XmiI GTMKAC 2 cut(s) 677, 1629
XmnI GAANNNNTTC 1 cut(s) 1695
XspI CTAG 5 cut(s) 1329, 1754, 2243, 2304, 2424
ZrmI AGTACT 2 cut(s) 833, 2314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.