RLG00000013091

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010996
Physical Location & Seq
Forward (+)
26129821 .. 26133684
3864 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013091

Sequence Viewer

Length: 2457 bp
ATGGGCTGCGCGAGCTCCAAGCTCGACGACCTGCCGGCGGTGGCGCTCTGCCGCGAGCGGTGTAGCTACCTCGACGAGGCGATTCACCAGCGCTACGCTCTCGCCGAGGCTCACATCGGTTATATTCACTCTCTCCGCGGCATCGGCCAGTCTCTCACTGATTTCATCAAACTAGAGGTCGCCAATTCCTCCGGCTCGCCGCCTTCTCCCACTCTCAACCTCCCTCCGGTCCGCAAAGGCGATCACGTCGCCGCCAAATCCTCCTCCCCCCACCACCTCTCCCACTCTAACTCCGGCTCCCACCTCCAGTTCCACTCAGATTCCGACGACGACTCCGTCTCGCTCCACCATTCCGACCACTCCTCGCCTCTGCACCTCCACGGCGGCATGGACTACATGGACGGCGGCGGCGGCGGCCAAGACGACATGAACCCCTACCCGCCGCAGGGGTATATGCACATGAACTACATGAGGAACAAAGCGACGCCGTCTGTGGTGTACCAGCAGAAACCATTGAGCCCCGAGAACGTGTACCATATGGGCGAATCCTCCAACTCATCTTACTACCCCTATCCTTACCAAGACGGCGGCGGCGGAGGCGGCGCCAATCCTTATCCCTATTCGGGTTACTCCAATTACGGCGGTGGAAGTAGCGGTGGGTATTATGGTAATTCCACACCCGCGCTTCCTCCTTACGGGGTGTCCTCGCCGCCAGTATCGCAGTCAAAGCCGCCGCCTCCGCCGCCTTCGCCGCCGAAAGCCTCGGCCTGGGACTTCATAAACCCGTTCGAGAGCATCGAGAGCTACTACAAGGCTTACACGCCGAGCCGGGACTCGAGGGAGGTCAGAGAAGAAGAGGGAATTCCCGATTTGGAAGACGAGGAGTACAACAACCACGGCGGCCACCACAGCCAGTACCAGCAAGAAGTGGTGAAGGAAGTTCACAGAGAGCACCACAAGGAAGGGAAGCAGCATCATCGTTCTTCGAAAGCCGTGGAGGATGAGGAGGTCGCCGAAGCTCAGGCCTCTCTGTACCAGACGAGGCCGAGCGTCGAGAACGAAAACGGGGTGGAGTATGAGGTACATGTGGTGGACAAGAAGGTTGTGGATGAGGAGGAGAAGCGTGAGGACCACAGGAGTTCCGGTGGAGGTGGTGGAGCATTCAAGCCGGGTTCTAGGGATGCTTTTGATGTGGCTAGAGAAATTCAAGCTCAGTTTCAGAGAGCTTCCGAGTCGGGTAACGAAATCGCAAGGATGCTGGAGGTGGGAACGCATCCGTATAATCGAAAACATGTTGCTTCCAAGATGATATCGCTAGTGTCGTCGCAGCCTTCTACTTCCAACAGTGTTGATGGGGCATCATCCTCAGAGGCTGGTCCTGCTCAACTGGCTTTTGATGAAGAATTGATGATGAGGACTAAAAATCTGTCTTCTACGTTGCACAAGCTCTATCTTTGGGAGAAGAAACTGTACAATGAAGTCAAGGCCGAGGAAAAGATGCGGGTGGTCCATGACAGGAAGTGTCGTAAGCTGAAGCGTTTAGATGAAAGGGGAGCAGAGGCCCAGAAAGTAGATACAACTAGAACTTTGATAAGGAGCTTGTCCACAAAAATAAAAATTGCAATTCAAGTTGTTGACAAGATATCTGTGACCATAAATAAGATCAGGGATGAAGAATTATGGCCACAATTAAACGAATTAATTCAAGGGTTAACCAGGATGTGGAAATCTATGCTTGAATGCCATCGTAATCAGTGCCTAGCAATCAGAGAGGCCAGGGGTTTAGGTCCTATTGGATCTGGAAAGAAGCTAAGTGATGCTCATGTTGATGCTACATTGCAATTTGAGCATGAGCTTGTTAACTGGACTTTCAGATTCTCTACCTGGATTAGCGCTCAAAAGGGTTATGTGAGAGCTTTGAATAATTGGCTTTTAAAGTGTCTTCTTTATGAACCTGAAGAAACAGCAGATGGAATAGTTCCCTTCTCACCTGGTCGAATAGGTGCGCCTCCTGTTTTTGTAATTTGTAACCAGTGGTCACAAGCTTTGGACAGGATATCAGAAAGAGAGGTGGTTGAGTCTATGCGTGTTTTCACCACGAGTGTGCTTCAGAGTTGGGAACAAGGTAAGCTTGAAATGCGGCAGAGAATGACGGAAAATCAAGATCTGCAAAAGAAATTTAAGAACTTGGATAAAAAGGACCAAAAGCTTCAGAAGGAGATTCAAGCATTAGACAAGAAAGTGGTGCTAGTCTCGGGGGATGTTGACTATCCCTCAGGTACTGGTGGACAGATTGTATATCAAAGTGAGACTAGAAATAGTACTTTACAGGCTAGTCTGCAGAGAATTTTTGAGGCCATGGAAAGGTTTACGGAAGTCTCCACAAAAGCTTACGAGGATCTTTTGCAACGCAGTGAAGAAGAAAGGCTTGCAGGGGAGCAGGAGAGAGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

819

Amino Acids

91.62

Weight (kDa)

5.98

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 1 - 57 7e-25 Protein of unknown function (DUF630)
DUF632 PF04782 397 - 702 3.3e-111 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35240 AT4G35240 AT4G35240 AT4G35240
fragaria_vesca FvH4_2g16330
malus_domestica MD05G1131700.v1.1 MD10G1134700.v1.1
prunus_persica Prupe.8G176200_v2.0.a1
pyrus_communis pycom05g12840 pycom10g11620
rosa_chinensis RchiOBHm_Chr6g0280091
rosa_laevigata RLG00000013091
rosa_multiflora Rmu_sc0007137.1_g000002
rosa_roxburghii Rroxscaffold_7G00188410
rosa_rugosa Rorug06G0129900
rosa_samantha Rh6AG240100 Rh6BG244000 Rh6CG245600 Rh6DG236500
rosa_wichuraiana Rw6G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 602
AccB7I CCANNNNNTGG 1 cut(s) 1722
AccBSI CCGCTC 1 cut(s) 58
AccII CGCG 4 cut(s) 11, 54, 138, 683
AclWI GGATC 2 cut(s) 1804, 2406
AcoI YGGCCR 4 cut(s) 145, 415, 901, 1682
AcsI RAATTY 4 cut(s) 861, 1203, 2177, 2346
AcuI CTGAAG 4 cut(s) 1553, 1977, 2093, 2195
AcyI GRCGYC 2 cut(s) 485, 603
AfaI GTAC 9 cut(s) 500, 533, 887, 917, 1034, 1083, 1472, 2281, 2323
AfeI AGCGCT 2 cut(s) 92, 1894
AflIII ACRYGT 3 cut(s) 528, 1084, 1291
AgsI TTSAA 8 cut(s) 1165, 1208, 1628, 1706, 1739, 1921, 2135, 2225
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 5 cut(s) 767, 1715, 1775, 1883, 1990
AleI CACNNNNGTG 1 cut(s) 2102
Alw21I GWGCWC 2 cut(s) 17, 954
Alw26I GTCTC 5 cut(s) 156, 343, 2257, 2303, 2383
AlwI GGATC 2 cut(s) 1804, 2406
AlwNI CAGNNNCTG 2 cut(s) 1373, 2282
Ama87I CYCGRG 3 cut(s) 521, 835, 2254
Aor51HI AGCGCT 2 cut(s) 92, 1894
ApeKI GCWGC 3 cut(s) 6, 970, 1327
ApoI RAATTY 4 cut(s) 861, 1203, 2177, 2346
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AseI ATTAAT 1 cut(s) 1700
Asp700I GAANNNNTTC 1 cut(s) 1701
AspLEI GCGC 7 cut(s) 11, 46, 93, 605, 685, 1895, 2008
AspS9I GGNCC 7 cut(s) 229, 1129, 1376, 1507, 1561, 1787, 2200
AsuC2I CCSGG 2 cut(s) 830, 1170
AsuHPI GGTGA 4 cut(s) 77, 943, 1980, 2086
AsuII TTCGAA 1 cut(s) 986
AvaI CYCGRG 3 cut(s) 521, 835, 2254
AvaII GGWCC 6 cut(s) 229, 1129, 1376, 1507, 1787, 2200
AxyI CCTNAGG 1 cut(s) 2275
BalI TGGCCA 1 cut(s) 1684
BanI GGYRCC 1 cut(s) 602
BanII GRGCYC 2 cut(s) 17, 521
BarI GAAGNNNNNNTAC 2 cut(s) 1454, 1486
BauI CACGAG 1 cut(s) 2098
BbsI GAAGAC 3 cut(s) 882, 1422, 1934
Bbv12I GWGCWC 2 cut(s) 17, 954
BbvI GCAGC 2 cut(s) 982, 1339
BccI CCATC 3 cut(s) 1346, 1752, 1964
BceAI ACGGC 7 cut(s) 397, 418, 472, 601, 655, 913, 977
BcgI CGANNNNNNTGC 2 cut(s) 959, 993
BciT130I CCWGG 5 cut(s) 769, 1717, 1777, 1885, 1992
BcnI CCSGG 2 cut(s) 830, 1170
BcoDI GTCTC 5 cut(s) 156, 343, 2257, 2303, 2383
BfaI CTAG 9 cut(s) 173, 1176, 1197, 1316, 1581, 1760, 2249, 2313, 2334
BfmI CTRYAG 1 cut(s) 2339
BfoI RGCGCY 4 cut(s) 47, 94, 606, 1896
BfuAI ACCTGC 1 cut(s) 39
BglII AGATCT 1 cut(s) 2164
BmcAI AGTACT 1 cut(s) 2323
Bme1390I CCNGG 7 cut(s) 769, 830, 1170, 1717, 1777, 1885, 1992
Bme18I GGWCC 6 cut(s) 229, 1129, 1376, 1507, 1787, 2200
BmeT110I CYCGRG 3 cut(s) 521, 835, 2254
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 7 cut(s) 229, 1129, 1376, 1507, 1561, 1787, 2200
BmiI GGNNCC 2 cut(s) 298, 604
BmrFI CCNGG 7 cut(s) 769, 830, 1170, 1717, 1777, 1885, 1992
BpiI GAAGAC 3 cut(s) 882, 1422, 1934
BpmI CTGGAG 2 cut(s) 290, 1280
Bpu10I CCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 986
BpuMI CCSGG 2 cut(s) 830, 1170
BsaHI GRCGYC 2 cut(s) 485, 603
BsaWI WCCGGW 2 cut(s) 226, 1142
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 8 cut(s) 148, 307, 713, 913, 1392, 1868, 2032, 2287
Bse21I CCTNAGG 1 cut(s) 2275
Bse3DI GCAATG 1 cut(s) 1835
BseBI CCWGG 5 cut(s) 769, 1717, 1777, 1885, 1992
BseGI GGATG 9 cut(s) 1006, 1114, 1186, 1260, 1273, 1361, 1675, 1725, 2266
BseMI GCAATG 1 cut(s) 1835
BseMII CTCAG 5 cut(s) 330, 1034, 1226, 1380, 2289
BseNI ACTGG 8 cut(s) 148, 307, 713, 913, 1392, 1868, 2032, 2287
BseRI GAGGAG 6 cut(s) 253, 352, 896, 1019, 1127, 1130
BseXI GCAGC 2 cut(s) 982, 1339
BsgI GTGCAG 1 cut(s) 356
Bsh1236I CGCG 4 cut(s) 11, 54, 138, 683
BshNI GGYRCC 1 cut(s) 602
BsiHKAI GWGCWC 2 cut(s) 17, 954
BsiHKCI CYCGRG 3 cut(s) 521, 835, 2254
BsiSI CCGG 7 cut(s) 35, 192, 227, 294, 829, 1143, 1169
BslFI GGGAC 2 cut(s) 785, 845
BsmAI GTCTC 5 cut(s) 156, 343, 2257, 2303, 2383
BsmBI CGTCTC 1 cut(s) 343
BsmFI GGGAC 2 cut(s) 785, 845
BsmI GAATGC 2 cut(s) 1160, 1745
BsoBI CYCGRG 3 cut(s) 521, 835, 2254
Bsp119I TTCGAA 1 cut(s) 986
Bsp1286I GDGCHC 3 cut(s) 17, 521, 954
Bsp1407I TGTACA 1 cut(s) 1470
Bsp143I GATC 5 cut(s) 241, 1662, 1796, 2164, 2398
Bsp19I CCATGG 1 cut(s) 2358
BspCNI CTCAG 5 cut(s) 329, 1033, 1225, 1379, 2288
BspFNI CGCG 4 cut(s) 11, 54, 138, 683
BspLI GGNNCC 2 cut(s) 298, 604
BspMAI CTGCAG 1 cut(s) 2343
BspMI ACCTGC 1 cut(s) 39
BspPI GGATC 2 cut(s) 1804, 2406
BspT104I TTCGAA 1 cut(s) 986
BspT107I GGYRCC 1 cut(s) 602
BsrBI CCGCTC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 1835
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 1470
BsrI ACTGG 8 cut(s) 148, 307, 713, 913, 1392, 1868, 2032, 2287
BssAI RCCGGY 1 cut(s) 34
BssMI GATC 5 cut(s) 241, 1662, 1796, 2164, 2398
BssNI GRCGYC 2 cut(s) 485, 603
BssSI CACGAG 1 cut(s) 2098
BssT1I CCWWGG 1 cut(s) 2358
Bst2BI CACGAG 1 cut(s) 2098
Bst2UI CCWGG 5 cut(s) 769, 1717, 1777, 1885, 1992
Bst4CI ACNGT 2 cut(s) 1346, 1470
Bst6I CTCTTC 1 cut(s) 849
BstACI GRCGYC 2 cut(s) 485, 603
BstAUI TGTACA 1 cut(s) 1470
BstBI TTCGAA 1 cut(s) 986
BstC8I GCNNGC 5 cut(s) 13, 36, 56, 197, 2430
BstDEI CTNAG 6 cut(s) 316, 1020, 1212, 1366, 1811, 2275
BstDSI CCRYGG 5 cut(s) 136, 379, 895, 993, 2358
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 9 cut(s) 1006, 1114, 1186, 1260, 1273, 1361, 1675, 1725, 2266
BstFNI CGCG 4 cut(s) 11, 54, 138, 683
BstH2I RGCGCY 4 cut(s) 47, 94, 606, 1896
BstHHI GCGC 7 cut(s) 11, 46, 93, 605, 685, 1895, 2008
BstKTI GATC 5 cut(s) 244, 1665, 1799, 2167, 2401
BstMAI GTCTC 5 cut(s) 156, 343, 2257, 2303, 2383
BstMBI GATC 5 cut(s) 241, 1662, 1796, 2164, 2398
BstNI CCWGG 5 cut(s) 769, 1717, 1777, 1885, 1992
BstNSI RCATGY 2 cut(s) 1088, 1295
BstSCI CCNGG 7 cut(s) 767, 828, 1168, 1715, 1775, 1883, 1990
BstSFI CTRYAG 1 cut(s) 2339
BstUI CGCG 4 cut(s) 11, 54, 138, 683
BstV1I GCAGC 2 cut(s) 982, 1339
BstV2I GAAGAC 3 cut(s) 882, 1422, 1934
BstX2I RGATCY 3 cut(s) 1796, 2164, 2398
BstYI RGATCY 3 cut(s) 1796, 2164, 2398
Bsu36I CCTNAGG 1 cut(s) 2275
BtgI CCRYGG 5 cut(s) 136, 379, 895, 993, 2358
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 9 cut(s) 1006, 1114, 1186, 1260, 1273, 1361, 1675, 1725, 2266
BtsI GCAGTG 1 cut(s) 2419
BtsIMutI CAGTG 5 cut(s) 156, 1351, 1760, 2039, 2419
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 5 cut(s) 13, 36, 56, 197, 2430
CaiI CAGNNNCTG 2 cut(s) 1373, 2282
CfoI GCGC 7 cut(s) 11, 46, 93, 605, 685, 1895, 2008
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 7 cut(s) 229, 1129, 1376, 1507, 1561, 1787, 2200
Cfr42I CCGCGG 1 cut(s) 139
CpoI CGGWCCG 1 cut(s) 229
CseI GACGC 2 cut(s) 493, 1039
CsiI ACCWGGT 1 cut(s) 1990
Csp6I GTAC 9 cut(s) 499, 532, 886, 916, 1033, 1082, 1471, 2280, 2322
CspI CGGWCCG 1 cut(s) 229
CviQI GTAC 9 cut(s) 499, 532, 886, 916, 1033, 1082, 1471, 2280, 2322
DdeI CTNAG 6 cut(s) 316, 1020, 1212, 1366, 1811, 2275
DinI GGCGCC 1 cut(s) 604
DpnI GATC 5 cut(s) 243, 1664, 1798, 2166, 2400
DpnII GATC 5 cut(s) 241, 1662, 1796, 2164, 2398
DraI TTTAAA 1 cut(s) 1935
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 4 cut(s) 145, 415, 901, 1682
Eam1104I CTCTTC 1 cut(s) 849
EarI CTCTTC 1 cut(s) 849
EciI GGCGGA 2 cut(s) 609, 729
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 2358
Eco147I AGGCCT 1 cut(s) 1025
Eco24I GRGCYC 2 cut(s) 17, 521
Eco32I GATATC 3 cut(s) 1311, 1644, 2058
Eco47I GGWCC 6 cut(s) 229, 1129, 1376, 1507, 1787, 2200
Eco47III AGCGCT 2 cut(s) 92, 1894
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 4 cut(s) 1553, 1977, 2093, 2195
Eco81I CCTNAGG 1 cut(s) 2275
Eco88I CYCGRG 3 cut(s) 521, 835, 2254
EcoICRI GAGCTC 1 cut(s) 15
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 1787
EcoRI GAATTC 1 cut(s) 861
EcoRII CCWGG 5 cut(s) 767, 1715, 1775, 1883, 1990
EcoRV GATATC 3 cut(s) 1311, 1644, 2058
EcoT14I CCWWGG 1 cut(s) 2358
EcoT38I GRGCYC 2 cut(s) 17, 521
EgeI GGCGCC 1 cut(s) 604
EheI GGCGCC 1 cut(s) 604
ErhI CCWWGG 1 cut(s) 2358
Esp3I CGTCTC 1 cut(s) 343
FalI AAGNNNNNCTT 4 cut(s) 2115, 2147, 2412, 2444
FaqI GGGAC 2 cut(s) 785, 845
FauI CCCGC 3 cut(s) 447, 688, 1494
FauNDI CATATG 1 cut(s) 537
FokI GGATG 9 cut(s) 1013, 1121, 1193, 1260, 1267, 1348, 1682, 1732, 2273
FriOI GRGCYC 2 cut(s) 17, 521
FspBI CTAG 9 cut(s) 173, 1176, 1197, 1316, 1581, 1760, 2249, 2313, 2334
GlaI GCGC 7 cut(s) 10, 45, 92, 604, 684, 1894, 2007
GsuI CTGGAG 2 cut(s) 290, 1280
HaeII RGCGCY 4 cut(s) 47, 94, 606, 1896
HapII CCGG 7 cut(s) 35, 192, 227, 294, 829, 1143, 1169
HgaI GACGC 2 cut(s) 493, 1039
HhaI GCGC 7 cut(s) 11, 46, 93, 605, 685, 1895, 2008
Hin1I GRCGYC 2 cut(s) 485, 603
Hin6I GCGC 7 cut(s) 9, 44, 91, 603, 683, 1893, 2006
HinP1I GCGC 7 cut(s) 9, 44, 91, 603, 683, 1893, 2006
HincII GTYRAC 4 cut(s) 1636, 1713, 1861, 2266
HindII GTYRAC 4 cut(s) 1636, 1713, 1861, 2266
HindIII AAGCTT 4 cut(s) 2043, 2129, 2207, 2388
HinfI GANTC 9 cut(s) 82, 320, 332, 545, 833, 1232, 1875, 2078, 2221
HpaI GTTAAC 2 cut(s) 1713, 1861
HpaII CCGG 7 cut(s) 35, 192, 227, 294, 829, 1143, 1169
HphI GGTGA 4 cut(s) 77, 943, 1980, 2086
Hpy188III TCNNGA 6 cut(s) 790, 799, 866, 1054, 1800, 2162
Hpy99I CGWCG 8 cut(s) 29, 77, 251, 329, 332, 487, 1055, 1327
HpyAV CCTTC 8 cut(s) 213, 756, 928, 956, 1093, 1341, 1993, 2209
HpyCH4III ACNGT 2 cut(s) 1346, 1470
HpyCH4IV ACGT 3 cut(s) 246, 528, 1436
HpyCH4V TGCA 9 cut(s) 373, 457, 1441, 1622, 1840, 2170, 2341, 2407, 2432
HpyF3I CTNAG 6 cut(s) 316, 1020, 1212, 1366, 1811, 2275
HpySE526I ACGT 3 cut(s) 246, 528, 1436
Hsp92I GRCGYC 2 cut(s) 485, 603
HspAI GCGC 7 cut(s) 9, 44, 91, 603, 683, 1893, 2006
KasI GGCGCC 1 cut(s) 602
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
KspAI GTTAAC 2 cut(s) 1713, 1861
KspI CCGCGG 1 cut(s) 139
Kzo9I GATC 5 cut(s) 241, 1662, 1796, 2164, 2398
LmnI GCTCC 7 cut(s) 20, 302, 348, 1157, 1553, 1596, 2437
Lsp1109I GCAGC 2 cut(s) 982, 1339
MabI ACCWGGT 1 cut(s) 1990
MaeI CTAG 9 cut(s) 173, 1176, 1197, 1316, 1581, 1760, 2249, 2313, 2334
MaeII ACGT 3 cut(s) 246, 528, 1436
MaeIII GTNAC 5 cut(s) 626, 1238, 1648, 2027, 2037
MalI GATC 5 cut(s) 243, 1664, 1798, 2166, 2400
MbiI CCGCTC 1 cut(s) 58
MboI GATC 5 cut(s) 241, 1662, 1796, 2164, 2398
MflI RGATCY 3 cut(s) 1796, 2164, 2398
MhlI GDGCHC 3 cut(s) 17, 521, 954
MlsI TGGCCA 1 cut(s) 1684
MluNI TGGCCA 1 cut(s) 1684
Mly113I GGCGCC 1 cut(s) 603
MlyI GAGTC 4 cut(s) 326, 827, 1241, 2087
MmeI TCCRAC 4 cut(s) 348, 378, 576, 1365
Mox20I TGGCCA 1 cut(s) 1684
MroNI GCCGGC 1 cut(s) 34
MroXI GAANNNNTTC 1 cut(s) 1701
MscI TGGCCA 1 cut(s) 1684
MseI TTAA 6 cut(s) 1691, 1700, 1712, 1860, 1934, 2181
MslI CAYNNNNRTG 2 cut(s) 1827, 2102
Msp20I TGGCCA 1 cut(s) 1684
MspA1I CMGCKG 1 cut(s) 138
MspI CCGG 7 cut(s) 35, 192, 227, 294, 829, 1143, 1169
MspR9I CCNGG 7 cut(s) 769, 830, 1170, 1717, 1777, 1885, 1992
Mva1269I GAATGC 2 cut(s) 1160, 1745
MvaI CCWGG 5 cut(s) 769, 1717, 1777, 1885, 1992
MvnI CGCG 4 cut(s) 11, 54, 138, 683
NaeI GCCGGC 1 cut(s) 36
NarI GGCGCC 1 cut(s) 603
NciI CCSGG 2 cut(s) 830, 1170
NcoI CCATGG 1 cut(s) 2358
NdeI CATATG 1 cut(s) 537
NdeII GATC 5 cut(s) 241, 1662, 1796, 2164, 2398
NgoMIV GCCGGC 1 cut(s) 34
NlaIV GGNNCC 2 cut(s) 298, 604
NmeAIII GCCGAG 5 cut(s) 130, 743, 849, 1071, 1513
NmuCI GTSAC 2 cut(s) 1648, 2037
NspI RCATGY 2 cut(s) 1088, 1295
NspV TTCGAA 1 cut(s) 986
OliI CACNNNNGTG 1 cut(s) 2102
PaeR7I CTCGAG 1 cut(s) 835
PceI AGGCCT 1 cut(s) 1025
PciI ACATGT 2 cut(s) 1084, 1291
PcsI WCGNNNNNNNCGW 4 cut(s) 102, 333, 795, 1319
PctI GAATGC 2 cut(s) 1160, 1745
PdiI GCCGGC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 1701
PfeI GAWTC 5 cut(s) 82, 320, 545, 1875, 2221
PflFI GACNNNGTC 2 cut(s) 335, 487
PflMI CCANNNNNTGG 1 cut(s) 1722
PleI GAGTC 4 cut(s) 326, 827, 1240, 2086
PluTI GGCGCC 1 cut(s) 606
PpsI GAGTC 4 cut(s) 326, 827, 1240, 2086
PpuMI RGGWCCY 1 cut(s) 1787
PscI ACATGT 2 cut(s) 1084, 1291
PshBI ATTAAT 1 cut(s) 1700
Psp124BI GAGCTC 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 1787
Psp6I CCWGG 5 cut(s) 767, 1715, 1775, 1883, 1990
PspGI CCWGG 5 cut(s) 767, 1715, 1775, 1883, 1990
PspN4I GGNNCC 2 cut(s) 298, 604
PspPI GGNCC 7 cut(s) 229, 1129, 1376, 1507, 1561, 1787, 2200
PspPPI RGGWCCY 1 cut(s) 1787
PspXI VCTCGAGB 1 cut(s) 835
PstI CTGCAG 1 cut(s) 2343
PstNI CAGNNNCTG 2 cut(s) 1373, 2282
PsuI RGATCY 3 cut(s) 1796, 2164, 2398
PsyI GACNNNGTC 2 cut(s) 335, 487
RsaI GTAC 9 cut(s) 500, 533, 887, 917, 1034, 1083, 1472, 2281, 2323
RsaNI GTAC 9 cut(s) 499, 532, 886, 916, 1033, 1082, 1471, 2280, 2322
RseI CAYNNNNRTG 2 cut(s) 1827, 2102
Rsr2I CGGWCCG 1 cut(s) 229
RsrII CGGWCCG 1 cut(s) 229
SacI GAGCTC 1 cut(s) 17
SacII CCGCGG 1 cut(s) 139
SaqAI TTAA 6 cut(s) 1691, 1700, 1712, 1860, 1934, 2181
Sau3AI GATC 5 cut(s) 241, 1662, 1796, 2164, 2398
Sau96I GGNCC 7 cut(s) 229, 1129, 1376, 1507, 1561, 1787, 2200
ScaI AGTACT 1 cut(s) 2323
SchI GAGTC 4 cut(s) 326, 827, 1241, 2087
ScrFI CCNGG 7 cut(s) 769, 830, 1170, 1717, 1777, 1885, 1992
SduI GDGCHC 3 cut(s) 17, 521, 954
SexAI ACCWGGT 1 cut(s) 1990
SfcI CTRYAG 1 cut(s) 2339
SfoI GGCGCC 1 cut(s) 604
Sfr274I CTCGAG 1 cut(s) 835
Sfr303I CCGCGG 1 cut(s) 139
SfuI TTCGAA 1 cut(s) 986
SgrBI CCGCGG 1 cut(s) 139
SinI GGWCC 6 cut(s) 229, 1129, 1376, 1507, 1787, 2200
SlaI CTCGAG 1 cut(s) 835
SmiMI CAYNNNNRTG 2 cut(s) 1827, 2102
SmlI CTYRAG 1 cut(s) 835
SmoI CTYRAG 1 cut(s) 835
SseBI AGGCCT 1 cut(s) 1025
SspDI GGCGCC 1 cut(s) 602
SspMI CTAG 9 cut(s) 173, 1176, 1197, 1316, 1581, 1760, 2249, 2313, 2334
SstI GAGCTC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 1025
StyD4I CCNGG 7 cut(s) 767, 828, 1168, 1715, 1775, 1883, 1990
StyI CCWWGG 1 cut(s) 2358
TaaI ACNGT 2 cut(s) 1346, 1470
TaiI ACGT 3 cut(s) 249, 531, 1439
TaqI TCGA 9 cut(s) 24, 72, 789, 798, 836, 986, 1053, 1285, 1996
TatI WGTACW 3 cut(s) 885, 1470, 2321
TfiI GAWTC 5 cut(s) 82, 320, 545, 1875, 2221
Tru1I TTAA 6 cut(s) 1691, 1700, 1712, 1860, 1934, 2181
Tru9I TTAA 6 cut(s) 1691, 1700, 1712, 1860, 1934, 2181
TscAI CASTG 5 cut(s) 163, 1351, 1760, 2039, 2419
TseFI GTSAC 2 cut(s) 1648, 2037
TseI GCWGC 3 cut(s) 6, 970, 1327
Tsp45I GTSAC 2 cut(s) 1648, 2037
TspGWI ACGGA 4 cut(s) 325, 1266, 2168, 2387
TspRI CASTG 5 cut(s) 163, 1351, 1760, 2039, 2419
Tth111I GACNNNGTC 2 cut(s) 335, 487
Van91I CCANNNNNTGG 1 cut(s) 1722
VpaK11BI GGWCC 6 cut(s) 229, 1129, 1376, 1507, 1787, 2200
VspI ATTAAT 1 cut(s) 1700
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 4 cut(s) 861, 1203, 2177, 2346
XceI RCATGY 2 cut(s) 1088, 1295
XhoI CTCGAG 1 cut(s) 835
XmnI GAANNNNTTC 1 cut(s) 1701
XspI CTAG 9 cut(s) 173, 1176, 1197, 1316, 1581, 1760, 2249, 2313, 2334
ZrmI AGTACT 1 cut(s) 2323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.