RchiOBHm_Chr6g0280091

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010996
Physical Location & Seq
Reverse (-)
43383679 .. 43388832
5154 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25118

Sequence Viewer

Length: 2442 bp
ATGGGCTGCGCGAGCTCCAAGCTCGACGACCTCCCCGCGGTGGCGCTCTGCCGCGAGCGGTGTAGCTACCTCGACGAGGCGATTCACCAGCGCTACGCTCTCGCCGAGGCCCACATCGGTTATATTCACTCTCTCCGCGGCATCGGCCAGTCTCTCACTCATTTCATCGAACTAGAGGTCGCCAATTCCTCCGGCTCGCCGCCTTCTCCTACTCTCAACCTCCCTCCGGTCCGCAAAGGCGATCACGTCGCCGCCAAATCCTCCGCCCCGCACCACCTCTCCCACTCTAACTCCGGCTCCCACCTCCAATTCCACTCAGATTCCGACGACGACTCCGTCGGCTCGCTCCACCATTCCGACCACTCCTCGCCTCTGCACCTCCACGGCGGCATGGACTACATGGACGGCGGCCAAGACGACATGAACCCCTACCCGCCGCAGGGGTATCTGCACATGAACTACATGAGGAACAAAGCGACGCCGTCTGTGGTGTACCAGCAGAAACCCATGAGCCCCGAGAACGTGTACCACATGGGCGAATCCTCCAACTCATCTTACTACCCCTATCCTTACCAAGACGGCGGCGGCGGAGGCGGCGCCAATCCTTATCCCTATTCCGGGTACTCCAATTACGGCGGTGGAAGTAGCGGTGGGTATTATGGTAATTCCACACCCGCGCCTCCTCCTTACGGGGTGTCCTCGCCGCCGGCTCCCCAGTCAAAGCCGCCGCCTCCGCCGCCTTCGCCGCCGAAAGCCTCGGCCTGGGACTTCATAAACCCGTTCGAGAGCATCGAGAGGTACTACTCGGCTTACACGCCGAGCCGGGACTCGAGGGAGGTCAGAGAGGAAGAGGGAATTCCCGATTTGGAAGACGAGGAGTACAACAACCACGGCGGCCACCACAGCCAGTACCAGCAGGAAGTGGTGAAGGAAGTTCACAGAGAGCACCACAAGGAAGGGAAGCAGCATCATCGTTCTTCGAAAGCCGTGGAGGATGAGGAGGTCGTCGAAGCTCAGGCCTCGCTGTACCAGACGAGGCCGAGCGTCGAGAAACAAAACGGGGTGGAGTATGAGGTACACGTGGTGGACAAGAAGGTTGTGGATGAGGAGGAGAAGCGTGAGGACCACCGGAGTTCCGGTGGAGCATTCAAGCCGGGTTCTAGGGATGCTTTTGATGTGGCTAGAGAAATTCAGGCTCAGTTTCAGAGAGCTTCCGAGTCGGGTAACGAAATCGCAAGGATGCTGGAGGTGGGAAGGCATCCGTATAATCGGAAACATGTTACTTCCAAGATGATATCGCTAGTGTCGTCGCAGCCTTCTACTTCCAACAGTGTTGATGGGGCATCATCCTCAGAGGCTGGTCCTGCTCAATTGGGGTTTGATGAAGAACTGATGATGAGGACTAAAAATCTGTCTTCTACGTTGCACAAGCTCTATCTTTGGGAGAAGAAGCTGTACAATGAAGTCAAGGCCGAGGAAAAGATGCGGGTGGTCCATGACAGGAAGTGTCGTAAGCTGAAGCGTTTAGATGAAAGGGGAGCAGAGGCTCAGAAAGTAGATACAACTCGAACTTTGATAAGGAGCTTGTCCACAAAAATAAAAATTGCGATTCAAGTTGTGGATAAAATATCTGTGACCATAAATAAGATCAGGGATGAAGAATTGTGGCCACAATTAAACGAATTAATTCAAGGGTTAACCAGGATGTGGAAATCTATGCTTGAATGCCATCGTAATCAGTGCCTAGCAATCAGAGAAGCCAGGGGTTTAGGTCCTATTGGATCTGGAAAGAAGCTAAGTGATGCTCATGTTGATGCTACATTGCAATTTGAGCATGAGCTTGTTAACTGGACTTTCAGATTCTCTACTTGGATTAGCGCTCAAAAGGGTTATGTGAGAGCTTTGAATAATTGGCTTTTAAAGTGTCTTCTTTATGAACCTGAAGAAACAGCAGATGGAATAGTTCCCTTCTCACCTGGTCGAATAGGTGCGCCTCCTATTTTTGTAATTTGTAACCAGTGGTCACAAGCTTTGGACAGGATATCAGAAAGAGAGGTGGTTGATTCTATGCGTGTTTTCACCATGAGTGTGCTTCAGAGTTGGGAACAAGGTAAGCTTGAAATGCGGCAGAGAATGACGGAAAATCAAGATCTGCAAAAGAAATTTAAGAACTTGGATAAAAAGGACCAAAAACTTCAGAAGGAGATTCAAGCATTAGACAAGAAAGTGGTGCTAGTCTCGCGGGATGTTGACTATCCCTCAGATACTGGTGGACAGATTGTATATCAAAGTGAGACTAGAAATAGTACTTTACAGGCTAGTCTGCAAAGAATTTTTGAGGCCATGGAAAGGTTTACGGAAGTCTCCACAAAAGCTTACGAGGATCTTTTGCAACGCAGTGAAGAAGTAAGGCTTGCAGGGGAGCATGAGAGAGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

813

Amino Acids

91.62

Weight (kDa)

6.14

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 1 - 58 3.4e-25 Protein of unknown function (DUF630)
DUF632 PF04782 392 - 697 9.9e-112 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35240 AT4G35240 AT4G35240 AT4G35240
fragaria_vesca FvH4_2g16330
malus_domestica MD05G1131700.v1.1 MD10G1134700.v1.1
prunus_persica Prupe.8G176200_v2.0.a1
pyrus_communis pycom05g12840 pycom10g11620
rosa_chinensis RchiOBHm_Chr6g0280091
rosa_laevigata RLG00000013091
rosa_multiflora Rmu_sc0007137.1_g000002
rosa_roxburghii Rroxscaffold_7G00188410
rosa_rugosa Rorug06G0129900
rosa_samantha Rh6AG240100 Rh6BG244000 Rh6CG245600 Rh6DG236500
rosa_wichuraiana Rw6G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 596
AccB7I CCANNNNNTGG 1 cut(s) 1707
AccBSI CCGCTC 1 cut(s) 58
AccII CGCG 6 cut(s) 11, 38, 54, 138, 677, 2242
AclWI GGATC 2 cut(s) 1789, 2391
AcoI YGGCCR 4 cut(s) 145, 409, 895, 1667
AcsI RAATTY 4 cut(s) 855, 1188, 2162, 2331
AcuI CTGAAG 4 cut(s) 1538, 1962, 2078, 2180
AcvI CACGTG 1 cut(s) 1081
AcyI GRCGYC 2 cut(s) 479, 597
AdeI CACNNNGTG 1 cut(s) 1084
AfeI AGCGCT 2 cut(s) 92, 1879
AflIII ACRYGT 3 cut(s) 522, 1078, 1276
AgsI TTSAA 7 cut(s) 1150, 1613, 1691, 1724, 1906, 2120, 2210
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 4 cut(s) 761, 1700, 1760, 1975
Alw21I GWGCWC 2 cut(s) 17, 948
Alw26I GTCTC 4 cut(s) 156, 2242, 2288, 2368
AlwI GGATC 2 cut(s) 1789, 2391
AlwNI CAGNNNCTG 2 cut(s) 1358, 2267
Ama87I CYCGRG 2 cut(s) 515, 829
Aor51HI AGCGCT 2 cut(s) 92, 1879
ApeKI GCWGC 3 cut(s) 6, 964, 1312
ApoI RAATTY 4 cut(s) 855, 1188, 2162, 2331
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AseI ATTAAT 1 cut(s) 1685
Asp700I GAANNNNTTC 1 cut(s) 1686
AspLEI GCGC 7 cut(s) 11, 46, 93, 599, 679, 1880, 1993
AspS9I GGNCC 7 cut(s) 109, 229, 1123, 1361, 1492, 1772, 2185
AsuC2I CCSGG 3 cut(s) 619, 824, 1155
AsuHPI GGTGA 4 cut(s) 77, 937, 1965, 2071
AsuII TTCGAA 1 cut(s) 980
AvaI CYCGRG 2 cut(s) 515, 829
AvaII GGWCC 6 cut(s) 229, 1123, 1361, 1492, 1772, 2185
BalI TGGCCA 1 cut(s) 1669
BanI GGYRCC 1 cut(s) 596
BanII GRGCYC 2 cut(s) 17, 515
BarI GAAGNNNNNNTAC 2 cut(s) 1439, 1471
BbrPI CACGTG 1 cut(s) 1081
BbsI GAAGAC 3 cut(s) 876, 1407, 1919
Bbv12I GWGCWC 2 cut(s) 17, 948
BbvI GCAGC 2 cut(s) 976, 1324
BccI CCATC 3 cut(s) 1331, 1737, 1949
BceAI ACGGC 7 cut(s) 400, 421, 466, 595, 649, 907, 971
BcgI CGANNNNNNTGC 2 cut(s) 953, 987
BciT130I CCWGG 4 cut(s) 763, 1702, 1762, 1977
BcnI CCSGG 3 cut(s) 619, 824, 1155
BcoDI GTCTC 4 cut(s) 156, 2242, 2288, 2368
BfaI CTAG 8 cut(s) 173, 1161, 1182, 1301, 1745, 2234, 2298, 2319
BfoI RGCGCY 4 cut(s) 47, 94, 600, 1881
BglII AGATCT 1 cut(s) 2149
BmcAI AGTACT 1 cut(s) 2308
Bme1390I CCNGG 7 cut(s) 619, 763, 824, 1155, 1702, 1762, 1977
Bme18I GGWCC 6 cut(s) 229, 1123, 1361, 1492, 1772, 2185
BmeT110I CYCGRG 2 cut(s) 515, 829
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 7 cut(s) 109, 229, 1123, 1361, 1492, 1772, 2185
BmiI GGNNCC 3 cut(s) 298, 598, 711
BmrFI CCNGG 7 cut(s) 619, 763, 824, 1155, 1702, 1762, 1977
BmrI ACTGGG 1 cut(s) 709
BmuI ACTGGG 1 cut(s) 709
BpiI GAAGAC 3 cut(s) 876, 1407, 1919
BpmI CTGGAG 1 cut(s) 1265
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu14I TTCGAA 1 cut(s) 980
BpuMI CCSGG 3 cut(s) 619, 824, 1155
BsaAI YACGTR 1 cut(s) 1081
BsaHI GRCGYC 2 cut(s) 479, 597
BsaWI WCCGGW 3 cut(s) 226, 1128, 1136
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 6 cut(s) 148, 715, 907, 1853, 2017, 2272
Bse3DI GCAATG 1 cut(s) 1820
BseBI CCWGG 4 cut(s) 763, 1702, 1762, 1977
BseGI GGATG 9 cut(s) 1000, 1108, 1171, 1245, 1258, 1346, 1660, 1710, 2251
BseMI GCAATG 1 cut(s) 1820
BseMII CTCAG 6 cut(s) 330, 1028, 1211, 1365, 1562, 2274
BseNI ACTGG 6 cut(s) 148, 715, 907, 1853, 2017, 2272
BseRI GAGGAG 6 cut(s) 355, 672, 890, 1013, 1121, 1124
BseXI GCAGC 2 cut(s) 976, 1324
BsgI GTGCAG 2 cut(s) 359, 434
Bsh1236I CGCG 6 cut(s) 11, 38, 54, 138, 677, 2242
BshNI GGYRCC 1 cut(s) 596
BsiHKAI GWGCWC 2 cut(s) 17, 948
BsiHKCI CYCGRG 2 cut(s) 515, 829
BsiSI CCGG 9 cut(s) 192, 227, 294, 618, 707, 823, 1129, 1137, 1154
BslFI GGGAC 2 cut(s) 779, 839
BsmAI GTCTC 4 cut(s) 156, 2242, 2288, 2368
BsmFI GGGAC 2 cut(s) 779, 839
BsmI GAATGC 2 cut(s) 1145, 1730
BsoBI CYCGRG 2 cut(s) 515, 829
Bsp119I TTCGAA 1 cut(s) 980
Bsp1286I GDGCHC 3 cut(s) 17, 515, 948
Bsp1407I TGTACA 1 cut(s) 1455
Bsp143I GATC 5 cut(s) 241, 1647, 1781, 2149, 2383
Bsp19I CCATGG 1 cut(s) 2343
BspCNI CTCAG 6 cut(s) 329, 1027, 1210, 1364, 1561, 2273
BspFNI CGCG 6 cut(s) 11, 38, 54, 138, 677, 2242
BspLI GGNNCC 3 cut(s) 298, 598, 711
BspPI GGATC 2 cut(s) 1789, 2391
BspT104I TTCGAA 1 cut(s) 980
BspT107I GGYRCC 1 cut(s) 596
BsrBI CCGCTC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 1820
BsrFI RCCGGY 1 cut(s) 706
BsrGI TGTACA 1 cut(s) 1455
BsrI ACTGG 6 cut(s) 148, 715, 907, 1853, 2017, 2272
BssAI RCCGGY 1 cut(s) 706
BssMI GATC 5 cut(s) 241, 1647, 1781, 2149, 2383
BssNI GRCGYC 2 cut(s) 479, 597
BssT1I CCWWGG 1 cut(s) 2343
Bst2UI CCWGG 4 cut(s) 763, 1702, 1762, 1977
Bst4CI ACNGT 1 cut(s) 1331
Bst6I CTCTTC 1 cut(s) 843
BstACI GRCGYC 2 cut(s) 479, 597
BstAUI TGTACA 1 cut(s) 1455
BstBAI YACGTR 1 cut(s) 1081
BstBI TTCGAA 1 cut(s) 980
BstC8I GCNNGC 6 cut(s) 13, 56, 197, 344, 708, 2415
BstDEI CTNAG 7 cut(s) 316, 1014, 1197, 1351, 1548, 1796, 2260
BstDSI CCRYGG 6 cut(s) 36, 136, 382, 889, 987, 2343
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 9 cut(s) 1000, 1108, 1171, 1245, 1258, 1346, 1660, 1710, 2251
BstFNI CGCG 6 cut(s) 11, 38, 54, 138, 677, 2242
BstH2I RGCGCY 4 cut(s) 47, 94, 600, 1881
BstHHI GCGC 7 cut(s) 11, 46, 93, 599, 679, 1880, 1993
BstKTI GATC 5 cut(s) 244, 1650, 1784, 2152, 2386
BstMAI GTCTC 4 cut(s) 156, 2242, 2288, 2368
BstMBI GATC 5 cut(s) 241, 1647, 1781, 2149, 2383
BstNI CCWGG 4 cut(s) 763, 1702, 1762, 1977
BstNSI RCATGY 1 cut(s) 1280
BstSCI CCNGG 7 cut(s) 617, 761, 822, 1153, 1700, 1760, 1975
BstUI CGCG 6 cut(s) 11, 38, 54, 138, 677, 2242
BstV1I GCAGC 2 cut(s) 976, 1324
BstV2I GAAGAC 3 cut(s) 876, 1407, 1919
BstX2I RGATCY 3 cut(s) 1781, 2149, 2383
BstYI RGATCY 3 cut(s) 1781, 2149, 2383
BtgI CCRYGG 6 cut(s) 36, 136, 382, 889, 987, 2343
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 9 cut(s) 1000, 1108, 1171, 1245, 1258, 1346, 1660, 1710, 2251
BtsI GCAGTG 1 cut(s) 2404
BtsIMutI CAGTG 4 cut(s) 1336, 1745, 2024, 2404
Cac8I GCNNGC 6 cut(s) 13, 56, 197, 344, 708, 2415
CaiI CAGNNNCTG 2 cut(s) 1358, 2267
CfoI GCGC 7 cut(s) 11, 46, 93, 599, 679, 1880, 1993
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 7 cut(s) 109, 229, 1123, 1361, 1492, 1772, 2185
Cfr42I CCGCGG 2 cut(s) 39, 139
CpoI CGGWCCG 1 cut(s) 229
CseI GACGC 2 cut(s) 487, 1033
CsiI ACCWGGT 1 cut(s) 1975
CspI CGGWCCG 1 cut(s) 229
DdeI CTNAG 7 cut(s) 316, 1014, 1197, 1351, 1548, 1796, 2260
DinI GGCGCC 1 cut(s) 598
DpnI GATC 5 cut(s) 243, 1649, 1783, 2151, 2385
DpnII GATC 5 cut(s) 241, 1647, 1781, 2149, 2383
DraI TTTAAA 1 cut(s) 1920
DraIII CACNNNGTG 1 cut(s) 1084
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 4 cut(s) 145, 409, 895, 1667
Eam1104I CTCTTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 843
EciI GGCGGA 3 cut(s) 253, 603, 723
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 2343
Eco147I AGGCCT 1 cut(s) 1019
Eco24I GRGCYC 2 cut(s) 17, 515
Eco32I GATATC 2 cut(s) 1296, 2043
Eco47I GGWCC 6 cut(s) 229, 1123, 1361, 1492, 1772, 2185
Eco47III AGCGCT 2 cut(s) 92, 1879
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 4 cut(s) 1538, 1962, 2078, 2180
Eco72I CACGTG 1 cut(s) 1081
Eco88I CYCGRG 2 cut(s) 515, 829
EcoICRI GAGCTC 1 cut(s) 15
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 1772
EcoRI GAATTC 1 cut(s) 855
EcoRII CCWGG 4 cut(s) 761, 1700, 1760, 1975
EcoRV GATATC 2 cut(s) 1296, 2043
EcoT14I CCWWGG 1 cut(s) 2343
EcoT38I GRGCYC 2 cut(s) 17, 515
EgeI GGCGCC 1 cut(s) 598
EheI GGCGCC 1 cut(s) 598
ErhI CCWWGG 1 cut(s) 2343
FalI AAGNNNNNCTT 4 cut(s) 2100, 2132, 2397, 2429
FaqI GGGAC 2 cut(s) 779, 839
FauI CCCGC 6 cut(s) 43, 276, 441, 682, 1479, 2235
FokI GGATG 9 cut(s) 1007, 1115, 1178, 1245, 1252, 1333, 1667, 1717, 2258
FriOI GRGCYC 2 cut(s) 17, 515
FspBI CTAG 8 cut(s) 173, 1161, 1182, 1301, 1745, 2234, 2298, 2319
GlaI GCGC 7 cut(s) 10, 45, 92, 598, 678, 1879, 1992
GsuI CTGGAG 1 cut(s) 1265
HaeII RGCGCY 4 cut(s) 47, 94, 600, 1881
HapII CCGG 9 cut(s) 192, 227, 294, 618, 707, 823, 1129, 1137, 1154
HgaI GACGC 2 cut(s) 487, 1033
HhaI GCGC 7 cut(s) 11, 46, 93, 599, 679, 1880, 1993
Hin1I GRCGYC 2 cut(s) 479, 597
Hin6I GCGC 7 cut(s) 9, 44, 91, 597, 677, 1878, 1991
HinP1I GCGC 7 cut(s) 9, 44, 91, 597, 677, 1878, 1991
HincII GTYRAC 3 cut(s) 1698, 1846, 2251
HindII GTYRAC 3 cut(s) 1698, 1846, 2251
HindIII AAGCTT 3 cut(s) 2028, 2114, 2373
HpaI GTTAAC 2 cut(s) 1698, 1846
HpaII CCGG 9 cut(s) 192, 227, 294, 618, 707, 823, 1129, 1137, 1154
HphI GGTGA 4 cut(s) 77, 937, 1965, 2071
Hpy188III TCNNGA 6 cut(s) 784, 793, 860, 1048, 1785, 2147
HpyAV CCTTC 9 cut(s) 213, 750, 922, 950, 1087, 1248, 1326, 1978, 2194
HpyCH4III ACNGT 1 cut(s) 1331
HpyCH4IV ACGT 4 cut(s) 246, 522, 1080, 1421
HpyCH4V TGCA 8 cut(s) 376, 451, 1426, 1825, 2155, 2326, 2392, 2417
HpyF3I CTNAG 7 cut(s) 316, 1014, 1197, 1351, 1548, 1796, 2260
HpySE526I ACGT 4 cut(s) 246, 522, 1080, 1421
Hsp92I GRCGYC 2 cut(s) 479, 597
HspAI GCGC 7 cut(s) 9, 44, 91, 597, 677, 1878, 1991
KasI GGCGCC 1 cut(s) 596
KroI GCCGGC 1 cut(s) 706
KroNI GCCGGC 1 cut(s) 708
KspAI GTTAAC 2 cut(s) 1698, 1846
KspI CCGCGG 2 cut(s) 39, 139
Kzo9I GATC 5 cut(s) 241, 1647, 1781, 2149, 2383
LmnI GCTCC 8 cut(s) 20, 302, 351, 715, 1142, 1538, 1581, 2422
Lsp1109I GCAGC 2 cut(s) 976, 1324
MabI ACCWGGT 1 cut(s) 1975
MaeI CTAG 8 cut(s) 173, 1161, 1182, 1301, 1745, 2234, 2298, 2319
MaeII ACGT 4 cut(s) 246, 522, 1080, 1421
MaeIII GTNAC 5 cut(s) 1223, 1279, 1633, 2012, 2022
MalI GATC 5 cut(s) 243, 1649, 1783, 2151, 2385
MbiI CCGCTC 1 cut(s) 58
MboI GATC 5 cut(s) 241, 1647, 1781, 2149, 2383
MfeI CAATTG 1 cut(s) 1370
MflI RGATCY 3 cut(s) 1781, 2149, 2383
MhlI GDGCHC 3 cut(s) 17, 515, 948
MlsI TGGCCA 1 cut(s) 1669
MluNI TGGCCA 1 cut(s) 1669
Mly113I GGCGCC 1 cut(s) 597
MlyI GAGTC 3 cut(s) 326, 821, 1226
MmeI TCCRAC 4 cut(s) 348, 381, 570, 1350
Mox20I TGGCCA 1 cut(s) 1669
MroNI GCCGGC 1 cut(s) 706
MroXI GAANNNNTTC 1 cut(s) 1686
MscI TGGCCA 1 cut(s) 1669
MseI TTAA 6 cut(s) 1676, 1685, 1697, 1845, 1919, 2166
MslI CAYNNNNRTG 2 cut(s) 1812, 2087
Msp20I TGGCCA 1 cut(s) 1669
MspA1I CMGCKG 2 cut(s) 38, 138
MspI CCGG 9 cut(s) 192, 227, 294, 618, 707, 823, 1129, 1137, 1154
MspR9I CCNGG 7 cut(s) 619, 763, 824, 1155, 1702, 1762, 1977
MunI CAATTG 1 cut(s) 1370
Mva1269I GAATGC 2 cut(s) 1145, 1730
MvaI CCWGG 4 cut(s) 763, 1702, 1762, 1977
MvnI CGCG 6 cut(s) 11, 38, 54, 138, 677, 2242
NaeI GCCGGC 1 cut(s) 708
NarI GGCGCC 1 cut(s) 597
NciI CCSGG 3 cut(s) 619, 824, 1155
NcoI CCATGG 1 cut(s) 2343
NdeII GATC 5 cut(s) 241, 1647, 1781, 2149, 2383
NgoMIV GCCGGC 1 cut(s) 706
NlaIV GGNNCC 3 cut(s) 298, 598, 711
NmeAIII GCCGAG 6 cut(s) 130, 737, 785, 843, 1065, 1498
NmuCI GTSAC 2 cut(s) 1633, 2022
NspI RCATGY 1 cut(s) 1280
NspV TTCGAA 1 cut(s) 980
PaeR7I CTCGAG 1 cut(s) 829
PceI AGGCCT 1 cut(s) 1019
PciI ACATGT 1 cut(s) 1276
PcsI WCGNNNNNNNCGW 4 cut(s) 102, 333, 789, 1304
PctI GAATGC 2 cut(s) 1145, 1730
PdiI GCCGGC 1 cut(s) 708
PdmI GAANNNNTTC 1 cut(s) 1686
PfeI GAWTC 7 cut(s) 82, 320, 539, 1609, 1860, 2063, 2206
PflFI GACNNNGTC 2 cut(s) 335, 481
PflMI CCANNNNNTGG 1 cut(s) 1707
PleI GAGTC 3 cut(s) 326, 821, 1225
PluTI GGCGCC 1 cut(s) 600
PmaCI CACGTG 1 cut(s) 1081
PmlI CACGTG 1 cut(s) 1081
PpsI GAGTC 3 cut(s) 326, 821, 1225
Ppu21I YACGTR 1 cut(s) 1081
PpuMI RGGWCCY 1 cut(s) 1772
PscI ACATGT 1 cut(s) 1276
PshBI ATTAAT 1 cut(s) 1685
Psp124BI GAGCTC 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 1772
Psp6I CCWGG 4 cut(s) 761, 1700, 1760, 1975
PspCI CACGTG 1 cut(s) 1081
PspGI CCWGG 4 cut(s) 761, 1700, 1760, 1975
PspN4I GGNNCC 3 cut(s) 298, 598, 711
PspPI GGNCC 7 cut(s) 109, 229, 1123, 1361, 1492, 1772, 2185
PspPPI RGGWCCY 1 cut(s) 1772
PspXI VCTCGAGB 1 cut(s) 829
PstNI CAGNNNCTG 2 cut(s) 1358, 2267
PsuI RGATCY 3 cut(s) 1781, 2149, 2383
PsyI GACNNNGTC 2 cut(s) 335, 481
RseI CAYNNNNRTG 2 cut(s) 1812, 2087
Rsr2I CGGWCCG 1 cut(s) 229
RsrII CGGWCCG 1 cut(s) 229
SacI GAGCTC 1 cut(s) 17
SacII CCGCGG 2 cut(s) 39, 139
SaqAI TTAA 6 cut(s) 1676, 1685, 1697, 1845, 1919, 2166
Sau3AI GATC 5 cut(s) 241, 1647, 1781, 2149, 2383
Sau96I GGNCC 7 cut(s) 109, 229, 1123, 1361, 1492, 1772, 2185
ScaI AGTACT 1 cut(s) 2308
SchI GAGTC 3 cut(s) 326, 821, 1226
ScrFI CCNGG 7 cut(s) 619, 763, 824, 1155, 1702, 1762, 1977
SduI GDGCHC 3 cut(s) 17, 515, 948
SexAI ACCWGGT 1 cut(s) 1975
SfoI GGCGCC 1 cut(s) 598
Sfr274I CTCGAG 1 cut(s) 829
Sfr303I CCGCGG 2 cut(s) 39, 139
SfuI TTCGAA 1 cut(s) 980
SgrBI CCGCGG 2 cut(s) 39, 139
SinI GGWCC 6 cut(s) 229, 1123, 1361, 1492, 1772, 2185
SlaI CTCGAG 1 cut(s) 829
SmiMI CAYNNNNRTG 2 cut(s) 1812, 2087
SmlI CTYRAG 1 cut(s) 829
SmoI CTYRAG 1 cut(s) 829
SseBI AGGCCT 1 cut(s) 1019
SspDI GGCGCC 1 cut(s) 596
SspMI CTAG 8 cut(s) 173, 1161, 1182, 1301, 1745, 2234, 2298, 2319
SstI GAGCTC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 1019
StyD4I CCNGG 7 cut(s) 617, 761, 822, 1153, 1700, 1760, 1975
StyI CCWWGG 1 cut(s) 2343
TaaI ACNGT 1 cut(s) 1331
TaiI ACGT 4 cut(s) 249, 525, 1083, 1424
TatI WGTACW 3 cut(s) 879, 1455, 2306
TfiI GAWTC 7 cut(s) 82, 320, 539, 1609, 1860, 2063, 2206
Tru1I TTAA 6 cut(s) 1676, 1685, 1697, 1845, 1919, 2166
Tru9I TTAA 6 cut(s) 1676, 1685, 1697, 1845, 1919, 2166
TscAI CASTG 4 cut(s) 1336, 1745, 2024, 2404
TseFI GTSAC 2 cut(s) 1633, 2022
TseI GCWGC 3 cut(s) 6, 964, 1312
Tsp45I GTSAC 2 cut(s) 1633, 2022
TspGWI ACGGA 4 cut(s) 325, 1251, 2153, 2372
TspRI CASTG 4 cut(s) 1336, 1745, 2024, 2404
Tth111I GACNNNGTC 2 cut(s) 335, 481
Van91I CCANNNNNTGG 1 cut(s) 1707
VpaK11BI GGWCC 6 cut(s) 229, 1123, 1361, 1492, 1772, 2185
VspI ATTAAT 1 cut(s) 1685
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 4 cut(s) 855, 1188, 2162, 2331
XceI RCATGY 1 cut(s) 1280
XhoI CTCGAG 1 cut(s) 829
XmnI GAANNNNTTC 1 cut(s) 1686
XspI CTAG 8 cut(s) 173, 1161, 1182, 1301, 1745, 2234, 2298, 2319
ZrmI AGTACT 1 cut(s) 2308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.