MD10G1134700.v1.1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010996
Physical Location & Seq
Reverse (-)
21673511 .. 21679042
5532 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1134700.v1.1.491

Sequence Viewer

Length: 2469 bp
ATGGGCTGCACGAGCTCCAAGCTCGACGATCTTCCGGCCGTCGCCTTATGCCGGGAGCGCTGCGGCTTCCTCGACGAGGCGATTCACCAGCGCTACGCCTTCGCCGAGGCCCACATCGCTTATATACACTCCCTCAGAGGAATCGGCCGCTCTCTCCATCTATTCATCGAACAGGAAGTGGGCAATTCATCCGGATCGCCGCCCTCCCCCCACCTCAACCTCCCGCCGGCCCGAAAAGGCCAGACTAAATCTTCGCCGCCGCGCCACTCTCACTCGAATTCAAATTCTGGGTCCCATCTCCAGTTTCACTCCGATTCCGACGACGATTCCGGCTCGCTCCACCACTCCGACCACTCGTCGCCGCTGCACGATACTCACCACGGCGGTCATATTGATTATATGGAGGGCGGCGTCCAGGAGGGTTTCAATTCGTACCCAGATGGGTTTATGCGCATGAATTACATGAAGAACAAAGCGACGCCTTCTGTGGTGCATCGGCAGAAACCCGTGAGCCCCGAAAAAGTGTATCAATTGGGTGAATCTTCTTCTTCTAATCCGTATTATGGATACCAAAACTCAAATTTTTATCCAGATGCTGTCTCTAATCCTTATTCCGATTACGGCGGTGGGTTTAACAATTACAACGGCGGGGGTGGTGGGTATAATTATGGTGGCGGGGGTGGTGGGTATACCAATTACGGTAATTCTTCTCCGCCGGTCCGTTACGGAGCACCGTCGCCACAGGCCTCAGCTTCGAGTTCGTCCAAGCCGGCTCCTCCGCCGCCGCCTTCACCGCCGAGAGCTTCAGCTTGGGATTTCTTAAACCCATTCGAGGCTTACGAAAAGTCCTACTCTGTTTACACTCCGAGTCGGGACTTAAAGGAGGTGAGAGACGAAGAGGGAATTCCGGAATTGGAAGATGAGGAGTACCAGCAGGAGGTAGTGAAGGAGGTGCAGAGGGACCACAAGCATGTGGTGGATGGCGGGAGGCATTCCAAGGCTATTCTGGACGATGAGGCAGCTGAAACGCAGCCCTCCTCCCTTTATCAAGCTCGGCCGAGCGTTGGGACCGATGGCATTGGGGTAGAGTATGAAGTAAATGTTGTGGAGAAGAAGATTGTGGATGAGGAGGAGAGGCGCGAGGACCTTGGCAATGGCGGAGATGCAGCTAAAGCTCGACCGGGGTCTCGAGATGCTTTTGAAGTTGCTCGAGAGATTGAGGTCCAGTTTCAGAGAGCTTCGGAGTCGGGGAACGAAATTGCCAAGATGCTCGAGGTGGGAAAGCTTCCGCATAATCGAAAACATGTTTCTTCCAAGATGATGTCTTTGGTCTCTTCGCAACCTTCTACTAGCGCCGAGCCATCGACCTCAGCTGGAGTGGCTGCTCCTGCGCAATTGGGGTTCGATGAAGAATTGATGTTGAGGTCCAAAAATCTCTCTTCCACCTTACACAAGCTATATCTTTGGGAGAAGAAACTCTACAATGAAGTAAAGGCTGAGGAAAAGATGCGGGTGATCCATGACAGGAAGGTTCGTAAGTTGAAGCATTTAGATGAAAGGGGCGCAGAAGCTCATAAAGTTGATAAAACTCGAACTTTAATAAGGAGTCTGTCCACAAAAATCAAAATTGCAATTCAAGTGGTAGACAAGTTATCTGTGACTATAAATAAGATCAGGGATGAAGAATTATGGCCGCAACTAAATGAATTGATTCAGGGGTTAACCAGGATGTGGAAATGTATGCTCGAATGTCATAGTAGCCAGTGCCAAGTGATTAGAGAAGCCAGAGGTTTAGGTCCTATCAGATCTGGATCTGGAAAAATGCTTAATGATGCTCATCTTGCTGCCACATTGCAGCTTGAACATGAGCTTATTAATTGGACTTTCAGATTCTCTACTTGGATTAGTGCGCAGAAGGGATATGTGCGAGCTTTGAACAAGTGGCTTTTAAAATGTCTTCTGTATGAACCAGAAGAAACAGCGGACGGAATAGTTCCCTTTTCACCTGGTAGGATAGGTGCACCCCCAATTTTCGTAATATGTAACCAGTGGTCACAGGCTTTGGAGAATGTATCAGAAAGGGAGGTGGTTGAGTCGATGCGTATTTTCACCATGAGTGTGCTTCAAACTTGGGAACAAGATAAGCTTGAAATGCGCCAGAGGATGGTGGCACAGAAGGATCTAGAGAAACAAGTTAAAAACTTGGATAGAAAAGACCAAAAAATACATAAGGAAATTCAGGCATTAGACAAGAAAATTATGCTAGCGTCAGGGGACGGTGACGGTCTCTCGGTTGCTGGACAGATTGTATATCAGAGTGACACTAGAAATATTACTTTACAGGCTAGTCTGCAACGCATTTTTGAGGCGATGGAAAGGTTCGCTGATTGCTCTACAAAAGTGTATGAGGAGCTTTTGCAACGTAATGAAGAAGAAAGGCTTGCTAGAGCGGATGAGAGAGCTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

823

Amino Acids

91.9

Weight (kDa)

6.13

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 1 - 59 1.4e-26 Protein of unknown function (DUF630)
DUF632 PF04782 401 - 707 3.1e-110 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35240 AT4G35240 AT4G35240 AT4G35240
fragaria_vesca FvH4_2g16330
malus_domestica MD05G1131700.v1.1 MD10G1134700.v1.1
prunus_persica Prupe.8G176200_v2.0.a1
pyrus_communis pycom05g12840 pycom10g11620
rosa_chinensis RchiOBHm_Chr6g0280091
rosa_laevigata RLG00000013091
rosa_multiflora Rmu_sc0007137.1_g000002
rosa_roxburghii Rroxscaffold_7G00188410
rosa_rugosa Rorug06G0129900
rosa_samantha Rh6AG240100 Rh6BG244000 Rh6CG245600 Rh6DG236500
rosa_wichuraiana Rw6G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 452, 1392, 1911
AccB7I CCANNNNNTGG 2 cut(s) 1731, 2164
AccBSI CCGCTC 2 cut(s) 150, 2450
AccI GTMKAC 2 cut(s) 689, 1644
AccII CGCG 2 cut(s) 262, 1140
AccIII TCCGGA 2 cut(s) 191, 907
AclWI GGATC 4 cut(s) 202, 1510, 1819, 2187
AcoI YGGCCR 4 cut(s) 36, 145, 1055, 1691
AcsI RAATTY 5 cut(s) 277, 283, 580, 903, 2235
AcuI CTGAAG 1 cut(s) 789
AcyI GRCGYC 2 cut(s) 411, 479
AfaI GTAC 2 cut(s) 434, 929
AfeI AGCGCT 2 cut(s) 59, 92
AfiI CCNNNNNNNGG 9 cut(s) 51, 76, 226, 563, 832, 937, 1064, 1731, 2164
AflIII ACRYGT 1 cut(s) 1303
AgsI TTSAA 9 cut(s) 282, 427, 1202, 1543, 1637, 1862, 1936, 2126, 2150
AhdI GACNNNNNGTC 1 cut(s) 355
AjnI CCWGG 3 cut(s) 414, 1724, 2005
Alw21I GWGCWC 3 cut(s) 17, 733, 2023
Alw26I GTCTC 5 cut(s) 604, 885, 1191, 1336, 2291
Alw44I GTGCAC 1 cut(s) 2019
AlwI GGATC 4 cut(s) 202, 1510, 1819, 2187
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 3 cut(s) 1188, 1209, 1271
Aor13HI TCCGGA 2 cut(s) 191, 907
Aor51HI AGCGCT 2 cut(s) 59, 92
AoxI GGCC 8 cut(s) 36, 108, 145, 228, 238, 744, 1055, 1691
ApaLI GTGCAC 1 cut(s) 2019
ApeKI GCWGC 9 cut(s) 6, 60, 364, 1019, 1030, 1166, 1382, 1844, 1855
ApoI RAATTY 5 cut(s) 277, 283, 580, 903, 2235
ArsI GACNNNNNNTTYG 4 cut(s) 235, 267, 275, 307
AseI ATTAAT 1 cut(s) 1875
Asp700I GAANNNNTTC 1 cut(s) 1710
AsuC2I CCSGG 2 cut(s) 53, 1182
AsuHPI GGTGA 9 cut(s) 77, 368, 548, 783, 898, 1525, 1995, 2101, 2291
AsuNHI GCTAGC 1 cut(s) 2263
AvaI CYCGRG 3 cut(s) 1188, 1209, 1271
AvaII GGWCC 8 cut(s) 291, 718, 961, 1068, 1144, 1222, 1425, 1796
BaeGI GKGCMC 1 cut(s) 2023
BanII GRGCYC 2 cut(s) 17, 515
BarI GAAGNNNNNNTAC 2 cut(s) 1463, 1495
BauI CACGAG 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 1949
Bbv12I GWGCWC 3 cut(s) 17, 733, 2023
BbvCI CCTCAGC 3 cut(s) 748, 1369, 1497
BbvI GCAGC 8 cut(s) 47, 351, 1031, 1042, 1178, 1369, 1831, 1867
BccI CCATC 8 cut(s) 165, 303, 434, 974, 1067, 1369, 2158, 2365
BceAI ACGGC 4 cut(s) 23, 397, 637, 661
BciT130I CCWGG 3 cut(s) 416, 1726, 2007
BciVI GTATCC 1 cut(s) 560
BcnI CCSGG 2 cut(s) 53, 1182
BcoDI GTCTC 5 cut(s) 604, 885, 1191, 1336, 2291
BfaI CTAG 6 cut(s) 1350, 2183, 2264, 2325, 2346, 2445
BfoI RGCGCY 3 cut(s) 61, 94, 1356
BfuI GTATCC 1 cut(s) 560
BglII AGATCT 1 cut(s) 1805
Bme1390I CCNGG 5 cut(s) 53, 416, 1182, 1726, 2007
Bme18I GGWCC 8 cut(s) 291, 718, 961, 1068, 1144, 1222, 1425, 1796
BmeRI GACNNNNNGTC 1 cut(s) 355
BmeT110I CYCGRG 3 cut(s) 1188, 1209, 1271
BmiI GGNNCC 5 cut(s) 292, 293, 774, 962, 1069
BmrFI CCNGG 5 cut(s) 53, 416, 1182, 1726, 2007
BmsI GCATC 8 cut(s) 502, 583, 1153, 1183, 1257, 1497, 1822, 2088
BmtI GCTAGC 1 cut(s) 2267
BoxI GACNNNNGTC 1 cut(s) 1183
BpiI GAAGAC 1 cut(s) 1949
BpmI CTGGAG 2 cut(s) 284, 1395
Bpu10I CCTNAGC 3 cut(s) 748, 1369, 1497
BpuMI CCSGG 2 cut(s) 53, 1182
BsaBI GATNNNNATC 2 cut(s) 1810, 1836
BsaHI GRCGYC 2 cut(s) 411, 479
BsaI GGTCTC 3 cut(s) 1191, 1336, 2291
BsaJI CCNNGG 5 cut(s) 105, 379, 996, 1147, 1181
BsaWI WCCGGW 2 cut(s) 191, 907
Bsc4I CCNNNNNNNGG 9 cut(s) 51, 76, 226, 563, 832, 937, 1064, 1731, 2164
Bse118I RCCGGY 3 cut(s) 226, 715, 769
Bse1I ACTGG 4 cut(s) 301, 1225, 1762, 2047
Bse3DI GCAATG 2 cut(s) 1159, 1850
Bse8I GATNNNNATC 2 cut(s) 1810, 1836
BseAI TCCGGA 2 cut(s) 191, 907
BseBI CCWGG 3 cut(s) 416, 1726, 2007
BseDI CCNNGG 5 cut(s) 105, 379, 996, 1147, 1181
BseGI GGATG 7 cut(s) 188, 985, 1129, 1684, 1734, 2169, 2458
BseJI GATNNNNATC 2 cut(s) 1810, 1836
BseLI CCNNNNNNNGG 9 cut(s) 51, 76, 226, 563, 832, 937, 1064, 1731, 2164
BseMI GCAATG 2 cut(s) 1159, 1850
BseMII CTCAG 4 cut(s) 148, 762, 1383, 1488
BseNI ACTGG 4 cut(s) 301, 1225, 1762, 2047
BseRI GAGGAG 6 cut(s) 765, 938, 1027, 1142, 1145, 2423
BseSI GKGCMC 1 cut(s) 2023
BseX3I CGGCCG 3 cut(s) 36, 145, 1055
BseXI GCAGC 8 cut(s) 47, 351, 1031, 1042, 1178, 1369, 1831, 1867
BsgI GTGCAG 2 cut(s) 350, 974
Bsh1236I CGCG 2 cut(s) 262, 1140
Bsh1285I CGRYCG 4 cut(s) 39, 148, 1058, 1181
BshFI GGCC 8 cut(s) 38, 110, 147, 230, 240, 746, 1057, 1693
BsiEI CGRYCG 4 cut(s) 39, 148, 1058, 1181
BsiHKAI GWGCWC 3 cut(s) 17, 733, 2023
BsiHKCI CYCGRG 3 cut(s) 1188, 1209, 1271
BsiSI CCGG 9 cut(s) 35, 52, 192, 227, 330, 716, 770, 908, 1181
BslFI GGGAC 5 cut(s) 277, 887, 974, 1081, 2288
BslI CCNNNNNNNGG 9 cut(s) 51, 76, 226, 563, 832, 937, 1064, 1731, 2164
BsmAI GTCTC 5 cut(s) 604, 885, 1191, 1336, 2291
BsmBI CGTCTC 1 cut(s) 885
BsmFI GGGAC 5 cut(s) 277, 887, 974, 1081, 2288
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 8 cut(s) 38, 110, 147, 230, 240, 746, 1057, 1693
Bso31I GGTCTC 3 cut(s) 1191, 1336, 2291
BsoBI CYCGRG 3 cut(s) 1188, 1209, 1271
Bsp1286I GDGCHC 4 cut(s) 17, 515, 733, 2023
Bsp13I TCCGGA 2 cut(s) 191, 907
Bsp143I GATC 7 cut(s) 28, 194, 1515, 1671, 1805, 1811, 2179
BspANI GGCC 8 cut(s) 38, 110, 147, 230, 240, 746, 1057, 1693
BspCNI CTCAG 4 cut(s) 147, 761, 1382, 1489
BspEI TCCGGA 2 cut(s) 191, 907
BspFNI CGCG 2 cut(s) 262, 1140
BspLI GGNNCC 5 cut(s) 292, 293, 774, 962, 1069
BspOI GCTAGC 1 cut(s) 2267
BspPI GGATC 4 cut(s) 202, 1510, 1819, 2187
BspTNI GGTCTC 3 cut(s) 1191, 1336, 2291
BsrBI CCGCTC 2 cut(s) 150, 2450
BsrDI GCAATG 2 cut(s) 1159, 1850
BsrFI RCCGGY 3 cut(s) 226, 715, 769
BsrI ACTGG 4 cut(s) 301, 1225, 1762, 2047
BssAI RCCGGY 3 cut(s) 226, 715, 769
BssECI CCNNGG 5 cut(s) 105, 379, 996, 1147, 1181
BssMI GATC 7 cut(s) 28, 194, 1515, 1671, 1805, 1811, 2179
BssNAI GTATAC 1 cut(s) 690
BssNI GRCGYC 2 cut(s) 411, 479
BssSI CACGAG 1 cut(s) 10
BssT1I CCWWGG 2 cut(s) 996, 1147
Bst1107I GTATAC 1 cut(s) 690
Bst2BI CACGAG 1 cut(s) 10
Bst2UI CCWGG 3 cut(s) 416, 1726, 2007
Bst4CI ACNGT 4 cut(s) 701, 735, 2279, 2285
Bst6I CTCTTC 3 cut(s) 891, 1339, 1444
BstACI GRCGYC 2 cut(s) 411, 479
BstC8I GCNNGC 6 cut(s) 228, 335, 771, 1929, 2265, 2442
BstDEI CTNAG 4 cut(s) 134, 748, 1369, 1497
BstDSI CCRYGG 1 cut(s) 379
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 7 cut(s) 188, 985, 1129, 1684, 1734, 2169, 2458
BstFNI CGCG 2 cut(s) 262, 1140
BstH2I RGCGCY 3 cut(s) 61, 94, 1356
BstKTI GATC 7 cut(s) 31, 197, 1518, 1674, 1808, 1814, 2182
BstMAI GTCTC 5 cut(s) 604, 885, 1191, 1336, 2291
BstMBI GATC 7 cut(s) 28, 194, 1515, 1671, 1805, 1811, 2179
BstMCI CGRYCG 4 cut(s) 39, 148, 1058, 1181
BstMWI GCNNNNNNNGC 9 cut(s) 12, 57, 116, 793, 1172, 1379, 1388, 1841, 2152
BstNI CCWGG 3 cut(s) 416, 1726, 2007
BstNSI RCATGY 2 cut(s) 974, 1307
BstPAI GACNNNNGTC 1 cut(s) 1183
BstSCI CCNGG 5 cut(s) 51, 414, 1180, 1724, 2005
BstSLI GKGCMC 1 cut(s) 2023
BstUI CGCG 2 cut(s) 262, 1140
BstV1I GCAGC 8 cut(s) 47, 351, 1031, 1042, 1178, 1369, 1831, 1867
BstV2I GAAGAC 1 cut(s) 1949
BstX2I RGATCY 3 cut(s) 1805, 1811, 2179
BstYI RGATCY 3 cut(s) 1805, 1811, 2179
BstZ17I GTATAC 1 cut(s) 690
BstZI CGGCCG 3 cut(s) 36, 145, 1055
BsuI GTATCC 1 cut(s) 560
BsuRI GGCC 8 cut(s) 38, 110, 147, 230, 240, 746, 1057, 1693
BtgI CCRYGG 1 cut(s) 379
BtgZI GCGATG 2 cut(s) 100, 2384
BtsCI GGATG 7 cut(s) 188, 985, 1129, 1684, 1734, 2169, 2458
BtsIMutI CAGTG 2 cut(s) 1769, 2054
Cac8I GCNNGC 6 cut(s) 228, 335, 771, 1929, 2265, 2442
CaiI CAGNNNCTG 1 cut(s) 596
Cfr10I RCCGGY 3 cut(s) 226, 715, 769
CpoI CGGWCCG 1 cut(s) 718
CseI GACGC 3 cut(s) 400, 487, 2256
CsiI ACCWGGT 1 cut(s) 2005
Csp6I GTAC 2 cut(s) 433, 928
CspCI CAANNNNNGTGG 2 cut(s) 1620, 1655
CspI CGGWCCG 1 cut(s) 718
CviAII CATG 7 cut(s) 454, 463, 971, 1304, 1520, 1865, 2113
CviQI GTAC 2 cut(s) 433, 928
DdeI CTNAG 4 cut(s) 134, 748, 1369, 1497
DpnI GATC 7 cut(s) 30, 196, 1517, 1673, 1807, 1813, 2181
DpnII GATC 7 cut(s) 28, 194, 1515, 1671, 1805, 1811, 2179
DraI TTTAAA 1 cut(s) 1950
DriI GACNNNNNGTC 1 cut(s) 355
EaeI YGGCCR 4 cut(s) 36, 145, 1055, 1691
EagI CGGCCG 3 cut(s) 36, 145, 1055
Eam1104I CTCTTC 3 cut(s) 891, 1339, 1444
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 3 cut(s) 891, 1339, 1444
EciI GGCGGA 3 cut(s) 702, 768, 1173
Ecl136II GAGCTC 1 cut(s) 15
EclXI CGGCCG 3 cut(s) 36, 145, 1055
Eco130I CCWWGG 2 cut(s) 996, 1147
Eco147I AGGCCT 1 cut(s) 746
Eco24I GRGCYC 2 cut(s) 17, 515
Eco31I GGTCTC 3 cut(s) 1191, 1336, 2291
Eco47I GGWCC 8 cut(s) 291, 718, 961, 1068, 1144, 1222, 1425, 1796
Eco47III AGCGCT 2 cut(s) 59, 92
Eco52I CGGCCG 3 cut(s) 36, 145, 1055
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 789
Eco88I CYCGRG 3 cut(s) 1188, 1209, 1271
EcoICRI GAGCTC 1 cut(s) 15
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 3 cut(s) 291, 1144, 1796
EcoRI GAATTC 2 cut(s) 277, 903
EcoRII CCWGG 3 cut(s) 414, 1724, 2005
EcoT14I CCWWGG 2 cut(s) 996, 1147
EcoT38I GRGCYC 2 cut(s) 17, 515
ErhI CCWWGG 2 cut(s) 996, 1147
Esp3I CGTCTC 1 cut(s) 885
FaeI CATG 7 cut(s) 457, 466, 974, 1307, 1523, 1868, 2116
FalI AAGNNNNNCTT 4 cut(s) 2130, 2162, 2424, 2456
FaqI GGGAC 5 cut(s) 277, 887, 974, 1081, 2288
FatI CATG 7 cut(s) 453, 462, 970, 1303, 1519, 1864, 2112
FauI CCCGC 5 cut(s) 231, 641, 668, 977, 1503
FblI GTMKAC 2 cut(s) 689, 1644
FokI GGATG 7 cut(s) 175, 992, 1136, 1691, 1741, 2176, 2465
FriOI GRGCYC 2 cut(s) 17, 515
FspAI RTGCGCAY 1 cut(s) 452
FspBI CTAG 6 cut(s) 1350, 2183, 2264, 2325, 2346, 2445
FspI TGCGCA 3 cut(s) 452, 1392, 1911
GsuI CTGGAG 2 cut(s) 284, 1395
HaeII RGCGCY 3 cut(s) 61, 94, 1356
HaeIII GGCC 8 cut(s) 38, 110, 147, 230, 240, 746, 1057, 1693
HapII CCGG 9 cut(s) 35, 52, 192, 227, 330, 716, 770, 908, 1181
HgaI GACGC 3 cut(s) 400, 487, 2256
Hin1I GRCGYC 2 cut(s) 411, 479
Hin1II CATG 7 cut(s) 457, 466, 974, 1307, 1523, 1868, 2116
HincII GTYRAC 1 cut(s) 1722
HindII GTYRAC 1 cut(s) 1722
HindIII AAGCTT 2 cut(s) 1283, 2144
HpaI GTTAAC 1 cut(s) 1722
HpaII CCGG 9 cut(s) 35, 52, 192, 227, 330, 716, 770, 908, 1181
HphI GGTGA 9 cut(s) 77, 368, 548, 783, 898, 1525, 1995, 2101, 2291
Hpy166II GTNNAC 6 cut(s) 690, 859, 1614, 1645, 1722, 2021
Hpy8I GTNNAC 6 cut(s) 690, 859, 1614, 1645, 1722, 2021
Hpy99I CGWCG 8 cut(s) 29, 44, 77, 323, 326, 361, 481, 739
HpyAV CCTTC 8 cut(s) 109, 492, 798, 940, 1353, 1522, 1909, 2170
HpyCH4III ACNGT 4 cut(s) 701, 735, 2279, 2285
HpyCH4IV ACGT 1 cut(s) 2422
HpyF10VI GCNNNNNNNGC 9 cut(s) 12, 57, 116, 793, 1172, 1379, 1388, 1841, 2152
HpyF3I CTNAG 4 cut(s) 134, 748, 1369, 1497
HpySE526I ACGT 1 cut(s) 2422
Hsp92I GRCGYC 2 cut(s) 411, 479
Hsp92II CATG 7 cut(s) 457, 466, 974, 1307, 1523, 1868, 2116
KflI GGGWCCC 1 cut(s) 291
Kpn2I TCCGGA 2 cut(s) 191, 907
KroI GCCGGC 2 cut(s) 226, 769
KroNI GCCGGC 2 cut(s) 228, 771
KspAI GTTAAC 1 cut(s) 1722
Kzo9I GATC 7 cut(s) 28, 194, 1515, 1671, 1805, 1811, 2179
LmnI GCTCC 7 cut(s) 20, 55, 342, 728, 778, 1390, 2410
Lsp1109I GCAGC 8 cut(s) 47, 351, 1031, 1042, 1178, 1369, 1831, 1867
LweI GCATC 8 cut(s) 502, 583, 1153, 1183, 1257, 1497, 1822, 2088
MabI ACCWGGT 1 cut(s) 2005
MaeI CTAG 6 cut(s) 1350, 2183, 2264, 2325, 2346, 2445
MaeII ACGT 1 cut(s) 2422
MaeIII GTNAC 6 cut(s) 722, 1657, 2042, 2052, 2279, 2318
MalI GATC 7 cut(s) 30, 196, 1517, 1673, 1807, 1813, 2181
MbiI CCGCTC 2 cut(s) 150, 2450
MboI GATC 7 cut(s) 28, 194, 1515, 1671, 1805, 1811, 2179
MfeI CAATTG 2 cut(s) 530, 1394
MflI RGATCY 3 cut(s) 1805, 1811, 2179
MhlI GDGCHC 4 cut(s) 17, 515, 733, 2023
MlyI GAGTC 4 cut(s) 877, 1253, 1615, 2102
MmeI TCCRAC 2 cut(s) 342, 372
MroI TCCGGA 2 cut(s) 191, 907
MroNI GCCGGC 2 cut(s) 226, 769
MroXI GAANNNNTTC 1 cut(s) 1710
MseI TTAA 9 cut(s) 633, 821, 878, 1598, 1721, 1827, 1875, 1949, 2196
MslI CAYNNNNRTG 3 cut(s) 969, 1551, 2117
MspA1I CMGCKG 4 cut(s) 364, 1022, 1373, 1982
MspI CCGG 9 cut(s) 35, 52, 192, 227, 330, 716, 770, 908, 1181
MspR9I CCNGG 5 cut(s) 53, 416, 1182, 1726, 2007
MunI CAATTG 2 cut(s) 530, 1394
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 3 cut(s) 416, 1726, 2007
MvnI CGCG 2 cut(s) 262, 1140
MwoI GCNNNNNNNGC 9 cut(s) 12, 57, 116, 793, 1172, 1379, 1388, 1841, 2152
NaeI GCCGGC 2 cut(s) 228, 771
NciI CCSGG 2 cut(s) 53, 1182
NdeII GATC 7 cut(s) 28, 194, 1515, 1671, 1805, 1811, 2179
NgoMIV GCCGGC 2 cut(s) 226, 769
NheI GCTAGC 1 cut(s) 2263
NlaIII CATG 7 cut(s) 457, 466, 974, 1307, 1523, 1868, 2116
NlaIV GGNNCC 5 cut(s) 292, 293, 774, 962, 1069
NmeAIII GCCGAG 5 cut(s) 130, 822, 1033, 1083, 1381
NmuCI GTSAC 4 cut(s) 1657, 2052, 2279, 2318
NsbI TGCGCA 3 cut(s) 452, 1392, 1911
NspI RCATGY 2 cut(s) 974, 1307
PaeR7I CTCGAG 3 cut(s) 1188, 1209, 1271
PceI AGGCCT 1 cut(s) 746
PciI ACATGT 1 cut(s) 1303
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 837
PctI GAATGC 1 cut(s) 991
PdiI GCCGGC 2 cut(s) 228, 771
PdmI GAANNNNTTC 1 cut(s) 1710
PfeI GAWTC 7 cut(s) 82, 141, 314, 326, 539, 1711, 1890
PflMI CCANNNNNTGG 2 cut(s) 1731, 2164
PfoI TCCNGGA 1 cut(s) 414
PleI GAGTC 4 cut(s) 876, 1252, 1614, 2101
PpsI GAGTC 4 cut(s) 876, 1252, 1614, 2101
PpuMI RGGWCCY 3 cut(s) 291, 1144, 1796
PscI ACATGT 1 cut(s) 1303
PshAI GACNNNNGTC 1 cut(s) 1183
PshBI ATTAAT 1 cut(s) 1875
Psp124BI GAGCTC 1 cut(s) 17
Psp5II RGGWCCY 3 cut(s) 291, 1144, 1796
Psp6I CCWGG 3 cut(s) 414, 1724, 2005
PspGI CCWGG 3 cut(s) 414, 1724, 2005
PspN4I GGNNCC 5 cut(s) 292, 293, 774, 962, 1069
PspPPI RGGWCCY 3 cut(s) 291, 1144, 1796
PspXI VCTCGAGB 1 cut(s) 1271
PstNI CAGNNNCTG 1 cut(s) 596
PsuI RGATCY 3 cut(s) 1805, 1811, 2179
PvuII CAGCTG 2 cut(s) 1022, 1373
RsaI GTAC 2 cut(s) 434, 929
RsaNI GTAC 2 cut(s) 433, 928
RseI CAYNNNNRTG 3 cut(s) 969, 1551, 2117
Rsr2I CGGWCCG 1 cut(s) 718
RsrII CGGWCCG 1 cut(s) 718
SacI GAGCTC 1 cut(s) 17
SaqAI TTAA 9 cut(s) 633, 821, 878, 1598, 1721, 1827, 1875, 1949, 2196
Sau3AI GATC 7 cut(s) 28, 194, 1515, 1671, 1805, 1811, 2179
SchI GAGTC 4 cut(s) 877, 1253, 1615, 2102
ScrFI CCNGG 5 cut(s) 53, 416, 1182, 1726, 2007
SduI GDGCHC 4 cut(s) 17, 515, 733, 2023
SexAI ACCWGGT 1 cut(s) 2005
SfaNI GCATC 8 cut(s) 502, 583, 1153, 1183, 1257, 1497, 1822, 2088
Sfr274I CTCGAG 3 cut(s) 1188, 1209, 1271
SinI GGWCC 8 cut(s) 291, 718, 961, 1068, 1144, 1222, 1425, 1796
SlaI CTCGAG 3 cut(s) 1188, 1209, 1271
SmiMI CAYNNNNRTG 3 cut(s) 969, 1551, 2117
SmlI CTYRAG 3 cut(s) 1188, 1209, 1271
SmoI CTYRAG 3 cut(s) 1188, 1209, 1271
SseBI AGGCCT 1 cut(s) 746
SspI AATATT 1 cut(s) 2332
SspMI CTAG 6 cut(s) 1350, 2183, 2264, 2325, 2346, 2445
SstI GAGCTC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 746
StyD4I CCNGG 5 cut(s) 51, 414, 1180, 1724, 2005
StyI CCWWGG 2 cut(s) 996, 1147
TaaI ACNGT 4 cut(s) 701, 735, 2279, 2285
TaiI ACGT 1 cut(s) 2425
TaqII GACCGA 1 cut(s) 1085
TfiI GAWTC 7 cut(s) 82, 141, 314, 326, 539, 1711, 1890
Tru1I TTAA 9 cut(s) 633, 821, 878, 1598, 1721, 1827, 1875, 1949, 2196
Tru9I TTAA 9 cut(s) 633, 821, 878, 1598, 1721, 1827, 1875, 1949, 2196
TscAI CASTG 2 cut(s) 1769, 2054
TseFI GTSAC 4 cut(s) 1657, 2052, 2279, 2318
TseI GCWGC 9 cut(s) 6, 60, 364, 1019, 1030, 1166, 1382, 1844, 1855
Tsp45I GTSAC 4 cut(s) 1657, 2052, 2279, 2318
TspGWI ACGGA 4 cut(s) 546, 710, 741, 2001
TspRI CASTG 2 cut(s) 1769, 2054
Van91I CCANNNNNTGG 2 cut(s) 1731, 2164
VneI GTGCAC 1 cut(s) 2019
VpaK11BI GGWCC 8 cut(s) 291, 718, 961, 1068, 1144, 1222, 1425, 1796
VspI ATTAAT 1 cut(s) 1875
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 5 cut(s) 277, 283, 580, 903, 2235
XbaI TCTAGA 1 cut(s) 2182
XceI RCATGY 2 cut(s) 974, 1307
XcmI CCANNNNNNNNNTGG 1 cut(s) 1003
XhoI CTCGAG 3 cut(s) 1188, 1209, 1271
XmiI GTMKAC 2 cut(s) 689, 1644
XmnI GAANNNNTTC 1 cut(s) 1710
XspI CTAG 6 cut(s) 1350, 2183, 2264, 2325, 2346, 2445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.