Prupe.8G176200_v2.0.a1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0010996
Physical Location & Seq
Reverse (-)
17826635 .. 17832438
5804 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G176200.1

Sequence Viewer

Length: 2442 bp
ATGGGCTGCACGAGCTCCAAGCTTGACGATCTTCCGGCCGTGGCTTTATGCCGAGAGCGCTGCGGCTATCTCGACGAGGCGATTCACCAGCGCTATGCTCTGGCAGAAGCCCACTTCGCTTATATTCACTCCCTCAGAGACATCGGCCACTCTCTGCATAAATTCATCGAACAGGAGGTGGGCAATTCATCTGGGTCACCTCCTTCTCCCCACCTCAACCTCCCTCCTGTCCGAAAAGGGGACACCAAATCGTCGCCGCTGCACCACTCTCATTCAAATTCAGGCTCCCATCTTCACTTTCACTCCGATTCCGACGACGACGACTTGGGCTCGCTCCACCACTCGGACCACTCGTCGCCGCTGCACAATACTCACCCTGGCAGCCATATTGATTACATGGGCAACGGCCAGGAGGGTTTCCAGGAGGGTTTTAGCTCGTACCCAGATGGTTACATGCGCATGAATTACATGAGGAACAAAGCGACACCGTCTGTGGTGTATCAGCAGAAACCCATGAGCCCAGAGAACGTGTATCACATGGGTGAATCTTCTTCTTCTTCAAATTCGCATTATGGATATCCAAATTCAAATCCTAATCCAAACAACACTGCTAATCCTTACCCCTATTATGGTGGGTACAACAATTACGGTGGCGGGGGTGGGGGGTATTATGGTAATTCATCTCCGCCGCCGCCTTACGGAGCCATATCGTCGCCGCCGGCTTCGGCTTCCACCTCTGCGAAGCCGCCTCCGCCTCCGCCATCGCCGCCTAGAGCTTCGGCTTGGGAGTTTTTGAACCCGTTCGAGACTTACGATAAGTACTACTCGGCCTACACGCCGAGCCGAGACTCGAAGGAAGTGAGAGACGAAGAGGGAATTCCCGATTTGGAAGACGAGGAGTACCAGCAGGAGGTGGTGAAGGAGGTGCAGAGAGACCACAAGCACGTGGTCGATGGCGGGAAGCATTCCAAGGCTGTTGTGGACGATGAGTTAGCTGAAACGCAGCCTTCCTCGCTTTATCAATCGAGGCCGAGCGTGGAGACCGATGGTGGTGGGGCTGAGTATGAGGTCCATGTGGTGGAGAAGAAGGTTGTGGATGAGGACGAGAGGCGCGAGGACCGTGGCAATGGCGGCGGTGCACCTAAAGTTCGACCGGGGTCTCGGGATGCTTTTGAAGTAGCTCGAGAGATTGAGGTTCAGTTTCAGAGAGCTTCCGAGTCCGGGAACGAAATTGCCAAGATGCTCGAGGTGGGAAGGCTTCCACATAATCGAAAACACGTTTCTTCTAAGATGATGTCGTTAGTGTCTTCGCAACCTTCTACTTCTAAGAGTGCTGAGCCATCGGCCTCCTCTGAAATAGCTGGTCCTGCTCAATTGGGGTTTGATGAAGAAATGATGATGAGGTCCAAAAATCTCTCTTCTACCTTAGCCAAGCTGTATCTTTGGGAGAAGAAACTCTATAATGAAGTAAAGTCTGAGGAAAAGATGCGGGTAATCCATGACAGGAAGGTCCGTAAGCTGAAGCGTTTAGATGAAAAGGGAGCGGAGGCTCACAAAGTTGATACAACTCGAACTTTAATAAGGAGTCTGTCCACAAAAATCAGAATCGCAATTCAAGTTGTTGACAAAATATCTGTGACCATAAATAAGATTAGGGATGAAGAATTATGGCCACAACTGAATGAATTAATTCAAGGGTTAACCCGGATGTGGAAATGCATGCTTGATTGTCATCGTACTCAGTGCCAAGTAATTAGAGAAGCCAGGGGTTTAGGTCCTATTGGATCTGGAAAAAGGCTTAGTGATGCTCATCTTGATGCTACATCGCAGCTTGAGCATGAGCTTATTAACTGGACTTTCAGATTCTCTACTTGGATTAGTGCTCAAAAGGGTTATGTGAGAGCTTTGAACAATTGGCTTCTAAAATGTCTTCTGTATGAACCCGAAGAAACACCAGATGGAATAGTTCCTTTTTCACCTGGTAGGATAGGTGCGCCTCCTGTTTTTGTAATCTGTAACCAATGGTCACAAGCTTTGGAGAGAATATCAGAAAGAGAGGTCGTTGAAACTATGCGTGTCTTCACCACGAGTGTCTTACAGGTTTGGGAACAAGATAAGCTTGAAATGCGCCAGAGGATGGTAGCACACAAGGATCTAGAGAAAAAAGTTAAGAACTTGGATAGAAAGGACCAAAAATTACAGAAGGAAATTCAGGCATTAGACAAGAAAATTGTGCTAGTACCAGGGGATGTTGACACTCTCTCAGGTGGTGGACAGATTGTATATCAGAGTGACACTAGAAATAGTACTTTACAGGCTAGTCTGCAACGCATTTTTGAGGCCATGGAAAGGTTCACCGATAACTCCACAAAAGCTTACGAGGAGCTTTTGCAACGTAACGAAGAAGATAGGCTTGCGAGAGAGGAAGAGAGGGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

814

Amino Acids

91.49

Weight (kDa)

6.04

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35240 AT4G35240 AT4G35240 AT4G35240
fragaria_vesca FvH4_2g16330
malus_domestica MD05G1131700.v1.1 MD10G1134700.v1.1
prunus_persica Prupe.8G176200_v2.0.a1
pyrus_communis pycom05g12840 pycom10g11620
rosa_chinensis RchiOBHm_Chr6g0280091
rosa_laevigata RLG00000013091
rosa_multiflora Rmu_sc0007137.1_g000002
rosa_roxburghii Rroxscaffold_7G00188410
rosa_rugosa Rorug06G0129900
rosa_samantha Rh6AG240100 Rh6BG244000 Rh6CG245600 Rh6DG236500
rosa_wichuraiana Rw6G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1508
Acc16I TGCGCA 1 cut(s) 458
AccB7I CCANNNNNTGG 2 cut(s) 1078, 2137
AccBSI CCGCTC 1 cut(s) 1544
AccII CGCG 1 cut(s) 1113
AclWI GGATC 2 cut(s) 1792, 2160
AcoI YGGCCR 4 cut(s) 36, 145, 406, 1670
AcsI RAATTY 6 cut(s) 161, 277, 562, 583, 876, 2208
AcuI CTGAAG 1 cut(s) 1541
AcvI CACGTG 1 cut(s) 946
AfaI GTAC 7 cut(s) 440, 638, 821, 902, 1738, 2241, 2308
AfeI AGCGCT 2 cut(s) 59, 92
AflIII ACRYGT 2 cut(s) 528, 1276
AhdI GACNNNNNGTC 1 cut(s) 352
AjnI CCWGG 6 cut(s) 376, 408, 420, 1763, 1978, 2242
AleI CACNNNNGTG 1 cut(s) 540
Alw21I GWGCWC 3 cut(s) 17, 1141, 1885
Alw26I GTCTC 7 cut(s) 132, 800, 840, 858, 927, 1034, 1164
Alw44I GTGCAC 1 cut(s) 1137
AlwI GGATC 2 cut(s) 1792, 2160
Ama87I CYCGRG 3 cut(s) 1161, 1182, 1244
Aor51HI AGCGCT 2 cut(s) 59, 92
AoxI GGCC 8 cut(s) 36, 145, 406, 828, 1028, 1344, 1670, 2340
ApaLI GTGCAC 1 cut(s) 1137
ApeKI GCWGC 7 cut(s) 6, 60, 259, 361, 381, 1003, 1828
ApoI RAATTY 6 cut(s) 161, 277, 562, 583, 876, 2208
AseI ATTAAT 1 cut(s) 1688
Asp700I GAANNNNTTC 2 cut(s) 800, 1689
AspLEI GCGC 6 cut(s) 60, 93, 459, 1113, 1996, 2130
AspS9I GGNCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
AsuC2I CCSGG 3 cut(s) 1155, 1222, 1705
AsuHPI GGTGA 8 cut(s) 77, 189, 365, 554, 928, 1968, 2074, 2347
AvaI CYCGRG 3 cut(s) 1161, 1182, 1244
AvaII GGWCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
BaeGI GKGCMC 1 cut(s) 1141
BalI TGGCCA 1 cut(s) 1672
BanII GRGCYC 3 cut(s) 17, 332, 521
BauI CACGAG 2 cut(s) 10, 2086
BbrPI CACGTG 1 cut(s) 946
BbsI GAAGAC 4 cut(s) 897, 1299, 1922, 2071
Bbv12I GWGCWC 3 cut(s) 17, 1141, 1885
BbvI GCAGC 6 cut(s) 47, 246, 348, 393, 1015, 1840
BccI CCATC 8 cut(s) 297, 440, 769, 947, 1040, 1348, 1952, 2131
BceAI ACGGC 2 cut(s) 23, 421
BcgI CGANNNNNNTGC 2 cut(s) 42, 76
BciT130I CCWGG 6 cut(s) 378, 410, 422, 1765, 1980, 2244
BcnI CCSGG 3 cut(s) 1155, 1222, 1705
BcoDI GTCTC 7 cut(s) 132, 800, 840, 858, 927, 1034, 1164
BfaI CTAG 5 cut(s) 771, 2156, 2237, 2298, 2319
BfoI RGCGCY 2 cut(s) 61, 94
BlpI GCTNAGC 1 cut(s) 1335
BmcAI AGTACT 2 cut(s) 821, 2308
Bme1390I CCNGG 9 cut(s) 378, 410, 422, 1155, 1222, 1705, 1765, 1980, 2244
Bme18I GGWCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
BmeRI GACNNNNNGTC 1 cut(s) 352
BmeT110I CYCGRG 3 cut(s) 1161, 1182, 1244
BmgT120I GGNCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
BmiI GGNNCC 2 cut(s) 286, 703
BmrFI CCNGG 9 cut(s) 378, 410, 422, 1155, 1222, 1705, 1765, 1980, 2244
BmsI GCATC 5 cut(s) 1156, 1230, 1476, 1795, 1807
BoxI GACNNNNGTC 1 cut(s) 1156
BpiI GAAGAC 4 cut(s) 897, 1299, 1922, 2071
Bpu10I CCTNAGC 1 cut(s) 1426
Bpu1102I GCTNAGC 1 cut(s) 1335
BpuEI CTTGAG 1 cut(s) 1853
BpuMI CCSGG 3 cut(s) 1155, 1222, 1705
BsaAI YACGTR 1 cut(s) 946
BsaBI GATNNNNATC 2 cut(s) 1731, 1809
BsaI GGTCTC 3 cut(s) 927, 1034, 1164
BsaJI CCNNGG 8 cut(s) 39, 376, 969, 1120, 1154, 1764, 2243, 2343
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bse118I RCCGGY 1 cut(s) 718
Bse1I ACTGG 1 cut(s) 1856
Bse3DI GCAATG 1 cut(s) 1132
Bse8I GATNNNNATC 2 cut(s) 1731, 1809
BseBI CCWGG 6 cut(s) 378, 410, 422, 1765, 1980, 2244
BseDI CCNNGG 8 cut(s) 39, 376, 969, 1120, 1154, 1764, 2243, 2343
BseGI GGATG 6 cut(s) 1102, 1171, 1663, 1713, 2142, 2254
BseJI GATNNNNATC 2 cut(s) 1731, 1809
BseMI GCAATG 1 cut(s) 1132
BseMII CTCAG 6 cut(s) 148, 1050, 1326, 1467, 1754, 2277
BseNI ACTGG 1 cut(s) 1856
BseRI GAGGAG 3 cut(s) 911, 1339, 2396
BseSI GKGCMC 1 cut(s) 1141
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 6 cut(s) 47, 246, 348, 393, 1015, 1840
BsgI GTGCAG 3 cut(s) 245, 347, 947
Bsh1236I CGCG 1 cut(s) 1113
Bsh1285I CGRYCG 2 cut(s) 39, 1154
BshFI GGCC 8 cut(s) 38, 147, 408, 830, 1030, 1346, 1672, 2342
BsiEI CGRYCG 2 cut(s) 39, 1154
BsiHKAI GWGCWC 3 cut(s) 17, 1141, 1885
BsiHKCI CYCGRG 3 cut(s) 1161, 1182, 1244
BsiSI CCGG 5 cut(s) 35, 719, 1154, 1221, 1705
BslFI GGGAC 1 cut(s) 254
BsmAI GTCTC 7 cut(s) 132, 800, 840, 858, 927, 1034, 1164
BsmBI CGTCTC 1 cut(s) 858
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 8 cut(s) 38, 147, 408, 830, 1030, 1346, 1672, 2342
Bso31I GGTCTC 3 cut(s) 927, 1034, 1164
BsoBI CYCGRG 3 cut(s) 1161, 1182, 1244
Bsp1286I GDGCHC 5 cut(s) 17, 332, 521, 1141, 1885
Bsp143I GATC 3 cut(s) 28, 1784, 2152
Bsp1720I GCTNAGC 1 cut(s) 1335
Bsp19I CCATGG 1 cut(s) 2343
BspANI GGCC 8 cut(s) 38, 147, 408, 830, 1030, 1346, 1672, 2342
BspCNI CTCAG 6 cut(s) 147, 1051, 1327, 1468, 1753, 2276
BspFNI CGCG 1 cut(s) 1113
BspLI GGNNCC 2 cut(s) 286, 703
BspPI GGATC 2 cut(s) 1792, 2160
BspTNI GGTCTC 3 cut(s) 927, 1034, 1164
BsrBI CCGCTC 1 cut(s) 1544
BsrDI GCAATG 1 cut(s) 1132
BsrFI RCCGGY 1 cut(s) 718
BsrI ACTGG 1 cut(s) 1856
BssAI RCCGGY 1 cut(s) 718
BssECI CCNNGG 8 cut(s) 39, 376, 969, 1120, 1154, 1764, 2243, 2343
BssMI GATC 3 cut(s) 28, 1784, 2152
BssSI CACGAG 2 cut(s) 10, 2086
BssT1I CCWWGG 2 cut(s) 969, 2343
Bst2BI CACGAG 2 cut(s) 10, 2086
Bst2UI CCWGG 6 cut(s) 378, 410, 422, 1765, 1980, 2244
Bst4CI ACNGT 3 cut(s) 489, 650, 1121
Bst6I CTCTTC 3 cut(s) 864, 1423, 2421
BstBAI YACGTR 1 cut(s) 946
BstC8I GCNNGC 4 cut(s) 332, 720, 1721, 2415
BstDSI CCRYGG 3 cut(s) 39, 1120, 2343
BstEII GGTNACC 1 cut(s) 195
BstF5I GGATG 6 cut(s) 1102, 1171, 1663, 1713, 2142, 2254
BstFNI CGCG 1 cut(s) 1113
BstH2I RGCGCY 2 cut(s) 61, 94
BstHHI GCGC 6 cut(s) 60, 93, 459, 1113, 1996, 2130
BstKTI GATC 3 cut(s) 31, 1787, 2155
BstMAI GTCTC 7 cut(s) 132, 800, 840, 858, 927, 1034, 1164
BstMBI GATC 3 cut(s) 28, 1784, 2152
BstMCI CGRYCG 2 cut(s) 39, 1154
BstNI CCWGG 6 cut(s) 378, 410, 422, 1765, 1980, 2244
BstNSI RCATGY 2 cut(s) 457, 1723
BstPAI GACNNNNGTC 1 cut(s) 1156
BstPI GGTNACC 1 cut(s) 195
BstSCI CCNGG 9 cut(s) 376, 408, 420, 1153, 1220, 1703, 1763, 1978, 2242
BstSLI GKGCMC 1 cut(s) 1141
BstUI CGCG 1 cut(s) 1113
BstV1I GCAGC 6 cut(s) 47, 246, 348, 393, 1015, 1840
BstV2I GAAGAC 4 cut(s) 897, 1299, 1922, 2071
BstX2I RGATCY 2 cut(s) 1784, 2152
BstYI RGATCY 2 cut(s) 1784, 2152
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 8 cut(s) 38, 147, 408, 830, 1030, 1346, 1672, 2342
BtgI CCRYGG 3 cut(s) 39, 1120, 2343
BtgZI GCGATG 2 cut(s) 747, 1809
BtsCI GGATG 6 cut(s) 1102, 1171, 1663, 1713, 2142, 2254
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 2 cut(s) 606, 1748
Cac8I GCNNGC 4 cut(s) 332, 720, 1721, 2415
CfoI GCGC 6 cut(s) 60, 93, 459, 1113, 1996, 2130
Cfr10I RCCGGY 1 cut(s) 718
Cfr13I GGNCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
CsiI ACCWGGT 1 cut(s) 1978
Csp6I GTAC 7 cut(s) 439, 637, 820, 901, 1737, 2240, 2307
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviQI GTAC 7 cut(s) 439, 637, 820, 901, 1737, 2240, 2307
DpnI GATC 3 cut(s) 30, 1786, 2154
DpnII GATC 3 cut(s) 28, 1784, 2152
DrdI GACNNNNNNGTC 1 cut(s) 1508
DriI GACNNNNNGTC 1 cut(s) 352
DseDI GACNNNNNNGTC 1 cut(s) 1508
EaeI YGGCCR 4 cut(s) 36, 145, 406, 1670
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 3 cut(s) 864, 1423, 2421
Eam1105I GACNNNNNGTC 1 cut(s) 352
EarI CTCTTC 3 cut(s) 864, 1423, 2421
EciI GGCGGA 3 cut(s) 675, 741, 747
Ecl136II GAGCTC 1 cut(s) 15
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 2 cut(s) 969, 2343
Eco24I GRGCYC 3 cut(s) 17, 332, 521
Eco31I GGTCTC 3 cut(s) 927, 1034, 1164
Eco32I GATATC 1 cut(s) 578
Eco47I GGWCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
Eco47III AGCGCT 2 cut(s) 59, 92
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 1541
Eco72I CACGTG 1 cut(s) 946
Eco88I CYCGRG 3 cut(s) 1161, 1182, 1244
Eco91I GGTNACC 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 1775
EcoO65I GGTNACC 1 cut(s) 195
EcoRI GAATTC 1 cut(s) 876
EcoRII CCWGG 6 cut(s) 376, 408, 420, 1763, 1978, 2242
EcoRV GATATC 1 cut(s) 578
EcoT14I CCWWGG 2 cut(s) 969, 2343
EcoT22I ATGCAT 1 cut(s) 1721
EcoT38I GRGCYC 3 cut(s) 17, 332, 521
ErhI CCWWGG 2 cut(s) 969, 2343
Esp3I CGTCTC 1 cut(s) 858
FalI AAGNNNNNCTT 4 cut(s) 2103, 2135, 2397, 2429
FaqI GGGAC 1 cut(s) 254
FauI CCCGC 3 cut(s) 647, 950, 1482
FokI GGATG 6 cut(s) 1109, 1178, 1670, 1720, 2149, 2261
FriOI GRGCYC 3 cut(s) 17, 332, 521
FspAI RTGCGCAY 1 cut(s) 458
FspBI CTAG 5 cut(s) 771, 2156, 2237, 2298, 2319
FspI TGCGCA 1 cut(s) 458
GlaI GCGC 6 cut(s) 59, 92, 458, 1112, 1995, 2129
HaeII RGCGCY 2 cut(s) 61, 94
HaeIII GGCC 8 cut(s) 38, 147, 408, 830, 1030, 1346, 1672, 2342
HapII CCGG 5 cut(s) 35, 719, 1154, 1221, 1705
HhaI GCGC 6 cut(s) 60, 93, 459, 1113, 1996, 2130
Hin6I GCGC 6 cut(s) 58, 91, 457, 1111, 1994, 2128
HinP1I GCGC 6 cut(s) 58, 91, 457, 1111, 1994, 2128
HincII GTYRAC 3 cut(s) 1624, 1701, 2254
HindII GTYRAC 3 cut(s) 1624, 1701, 2254
HindIII AAGCTT 4 cut(s) 20, 2031, 2117, 2373
HinfI GANTC 8 cut(s) 82, 308, 545, 848, 1217, 1585, 1605, 1863
HpaI GTTAAC 1 cut(s) 1701
HpaII CCGG 5 cut(s) 35, 719, 1154, 1221, 1705
HphI GGTGA 8 cut(s) 77, 189, 365, 554, 928, 1968, 2074, 2347
Hpy166II GTNNAC 8 cut(s) 982, 1139, 1593, 1624, 1701, 2254, 2273, 2355
Hpy188III TCNNGA 8 cut(s) 71, 805, 881, 1163, 1184, 1788, 1814, 2156
Hpy8I GTNNAC 8 cut(s) 982, 1139, 1593, 1624, 1701, 2254, 2273, 2355
Hpy99I CGWCG 7 cut(s) 77, 256, 317, 320, 323, 358, 715
HpyAV CCTTC 9 cut(s) 213, 847, 913, 1017, 1081, 1248, 1326, 1501, 2197
HpyCH4III ACNGT 3 cut(s) 489, 650, 1121
HpyCH4IV ACGT 4 cut(s) 528, 945, 1278, 2395
HpyCH4V TGCA 9 cut(s) 9, 157, 262, 364, 928, 1139, 1719, 2326, 2392
HpySE526I ACGT 4 cut(s) 528, 945, 1278, 2395
HspAI GCGC 6 cut(s) 58, 91, 457, 1111, 1994, 2128
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 1701
Kzo9I GATC 3 cut(s) 28, 1784, 2152
LmnI GCTCC 6 cut(s) 20, 290, 339, 701, 1541, 2383
Lsp1109I GCAGC 6 cut(s) 47, 246, 348, 393, 1015, 1840
LweI GCATC 5 cut(s) 1156, 1230, 1476, 1795, 1807
MabI ACCWGGT 1 cut(s) 1978
MaeI CTAG 5 cut(s) 771, 2156, 2237, 2298, 2319
MaeII ACGT 4 cut(s) 528, 945, 1278, 2395
MaeIII GTNAC 7 cut(s) 195, 449, 1636, 2015, 2025, 2291, 2396
MalI GATC 3 cut(s) 30, 1786, 2154
MbiI CCGCTC 1 cut(s) 1544
MboI GATC 3 cut(s) 28, 1784, 2152
MfeI CAATTG 2 cut(s) 1373, 1912
MflI RGATCY 2 cut(s) 1784, 2152
MhlI GDGCHC 5 cut(s) 17, 332, 521, 1141, 1885
MlsI TGGCCA 1 cut(s) 1672
MluNI TGGCCA 1 cut(s) 1672
MlyI GAGTC 3 cut(s) 842, 1226, 1594
MmeI TCCRAC 1 cut(s) 336
Mox20I TGGCCA 1 cut(s) 1672
Mph1103I ATGCAT 1 cut(s) 1721
MroNI GCCGGC 1 cut(s) 718
MroXI GAANNNNTTC 2 cut(s) 800, 1689
MscI TGGCCA 1 cut(s) 1672
MseI TTAA 5 cut(s) 1577, 1688, 1700, 1848, 2169
MslI CAYNNNNRTG 3 cut(s) 458, 540, 1815
Msp20I TGGCCA 1 cut(s) 1672
MspA1I CMGCKG 2 cut(s) 259, 361
MspI CCGG 5 cut(s) 35, 719, 1154, 1221, 1705
MspR9I CCNGG 9 cut(s) 378, 410, 422, 1155, 1222, 1705, 1765, 1980, 2244
MunI CAATTG 2 cut(s) 1373, 1912
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 6 cut(s) 378, 410, 422, 1765, 1980, 2244
MvnI CGCG 1 cut(s) 1113
NaeI GCCGGC 1 cut(s) 720
NciI CCSGG 3 cut(s) 1155, 1222, 1705
NcoI CCATGG 1 cut(s) 2343
NdeII GATC 3 cut(s) 28, 1784, 2152
NgoMIV GCCGGC 1 cut(s) 718
NlaIV GGNNCC 2 cut(s) 286, 703
NmeAIII GCCGAG 5 cut(s) 77, 806, 864, 869, 1056
NmuCI GTSAC 4 cut(s) 195, 1636, 2025, 2291
NsbI TGCGCA 1 cut(s) 458
NsiI ATGCAT 1 cut(s) 1721
NspI RCATGY 2 cut(s) 457, 1723
OliI CACNNNNGTG 1 cut(s) 540
PaeI GCATGC 1 cut(s) 1723
PaeR7I CTCGAG 2 cut(s) 1182, 1244
PcsI WCGNNNNNNNCGW 3 cut(s) 350, 810, 1110
PctI GAATGC 1 cut(s) 964
PdiI GCCGGC 1 cut(s) 720
PdmI GAANNNNTTC 2 cut(s) 800, 1689
PfeI GAWTC 5 cut(s) 82, 308, 545, 1605, 1863
PflFI GACNNNGTC 1 cut(s) 487
PflMI CCANNNNNTGG 2 cut(s) 1078, 2137
PfoI TCCNGGA 2 cut(s) 420, 1220
PleI GAGTC 3 cut(s) 842, 1225, 1593
PmaCI CACGTG 1 cut(s) 946
PmlI CACGTG 1 cut(s) 946
PpsI GAGTC 3 cut(s) 842, 1225, 1593
Ppu21I YACGTR 1 cut(s) 946
PpuMI RGGWCCY 1 cut(s) 1775
PshAI GACNNNNGTC 1 cut(s) 1156
PshBI ATTAAT 1 cut(s) 1688
Psp124BI GAGCTC 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 1775
Psp6I CCWGG 6 cut(s) 376, 408, 420, 1763, 1978, 2242
PspCI CACGTG 1 cut(s) 946
PspEI GGTNACC 1 cut(s) 195
PspGI CCWGG 6 cut(s) 376, 408, 420, 1763, 1978, 2242
PspN4I GGNNCC 2 cut(s) 286, 703
PspPI GGNCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
PspPPI RGGWCCY 1 cut(s) 1775
PspXI VCTCGAGB 1 cut(s) 1244
PsuI RGATCY 2 cut(s) 1784, 2152
PsyI GACNNNGTC 1 cut(s) 487
RsaI GTAC 7 cut(s) 440, 638, 821, 902, 1738, 2241, 2308
RsaNI GTAC 7 cut(s) 439, 637, 820, 901, 1737, 2240, 2307
RseI CAYNNNNRTG 3 cut(s) 458, 540, 1815
SacI GAGCTC 1 cut(s) 17
SaqAI TTAA 5 cut(s) 1577, 1688, 1700, 1848, 2169
Sau3AI GATC 3 cut(s) 28, 1784, 2152
Sau96I GGNCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
ScaI AGTACT 2 cut(s) 821, 2308
SchI GAGTC 3 cut(s) 842, 1226, 1594
ScrFI CCNGG 9 cut(s) 378, 410, 422, 1155, 1222, 1705, 1765, 1980, 2244
SduI GDGCHC 5 cut(s) 17, 332, 521, 1141, 1885
SexAI ACCWGGT 1 cut(s) 1978
SfaNI GCATC 5 cut(s) 1156, 1230, 1476, 1795, 1807
Sfr274I CTCGAG 2 cut(s) 1182, 1244
SinI GGWCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
SlaI CTCGAG 2 cut(s) 1182, 1244
SmiMI CAYNNNNRTG 3 cut(s) 458, 540, 1815
SmlI CTYRAG 3 cut(s) 1182, 1244, 1832
SmoI CTYRAG 3 cut(s) 1182, 1244, 1832
SphI GCATGC 1 cut(s) 1723
SspMI CTAG 5 cut(s) 771, 2156, 2237, 2298, 2319
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 9 cut(s) 376, 408, 420, 1153, 1220, 1703, 1763, 1978, 2242
StyI CCWWGG 2 cut(s) 969, 2343
TaaI ACNGT 3 cut(s) 489, 650, 1121
TaiI ACGT 4 cut(s) 531, 948, 1281, 2398
TaqII GACCGA 1 cut(s) 1058
TatI WGTACW 2 cut(s) 819, 2306
TauI GCSGC 9 cut(s) 66, 259, 361, 691, 694, 718, 748, 769, 1134
TfiI GAWTC 5 cut(s) 82, 308, 545, 1605, 1863
Tru1I TTAA 5 cut(s) 1577, 1688, 1700, 1848, 2169
Tru9I TTAA 5 cut(s) 1577, 1688, 1700, 1848, 2169
TscAI CASTG 2 cut(s) 613, 1748
TseFI GTSAC 4 cut(s) 195, 1636, 2025, 2291
TseI GCWGC 7 cut(s) 6, 60, 259, 361, 381, 1003, 1828
Tsp45I GTSAC 4 cut(s) 195, 1636, 2025, 2291
TspGWI ACGGA 2 cut(s) 714, 1502
TspRI CASTG 2 cut(s) 613, 1748
Tth111I GACNNNGTC 1 cut(s) 487
Van91I CCANNNNNTGG 2 cut(s) 1078, 2137
VneI GTGCAC 1 cut(s) 1137
VpaK11BI GGWCC 8 cut(s) 346, 1069, 1117, 1364, 1404, 1510, 1775, 2188
VspI ATTAAT 1 cut(s) 1688
XapI RAATTY 6 cut(s) 161, 277, 562, 583, 876, 2208
XbaI TCTAGA 1 cut(s) 2155
XceI RCATGY 2 cut(s) 457, 1723
XcmI CCANNNNNNNNNTGG 1 cut(s) 976
XhoI CTCGAG 2 cut(s) 1182, 1244
XmnI GAANNNNTTC 2 cut(s) 800, 1689
XspI CTAG 5 cut(s) 771, 2156, 2237, 2298, 2319
ZrmI AGTACT 2 cut(s) 821, 2308
Zsp2I ATGCAT 1 cut(s) 1721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.