MD05G1132900.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010779
Physical Location & Seq
Reverse (-)
25859908 .. 25867856
7949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1132900.v1.1.491

Sequence Viewer

Length: 1815 bp
ATGTTACCGGCGATGAAGAAGAAGAAGAGAGACGCAAACAACGGCCACCGCTGCATCTACATTAACGACGTTATCGCCGCCGCCGCCATAGGCGGCGTCATTTTCAGAACCTCTATTCTTCGGCGACACCTCCGCCGTTTCCGCCACCACGTGTTCCCGCTTATCTGCGCCGTATCCGGTTGCCTGCTTATCTTCCTCGCTGCCTTCTCTCTCCTCGCTCCGTCTCCGGTTCTGCATTTCCACAATCACCTCTCGCTCTTGCGTCACCTCTCCTCGGTTGACAATGGCGGCGATGAGGTCCGGGATAATTCGGATGAGACGACGACGTTTCGTGTGCCGGAGAGTGGAGGAAGTTCAGGTCGAGATTTATGGAGTACAAGGAATTCGGAGCTTTTCTTCGGTTGCAGCAATGCCGCCGATAACTTTGCAACTGCTGATGCGAAAACTCATTCGAATCGCTACTTGTTGATTGCTACAAGTGGAGGTTTGAACCAGCAAAGAACAGGGATAACAGATGCTGTCGTTGCAGCTTATATCTTGAATGCTACTCTTGTTGTTCCCAAGCTGGACCAGAAATCGTATTGGAAGGATTCCAGTAACTTTGATGAAATCTTTGACGTAGACTGGTTTATATCATCTCTCTCAAAAGATGTTAAAATCATAAAACAGCTTCCAACAAAGGGAGGGAAACCAATGAGCCCATATACCATGCGAGTTCCGAGGAAGTGTAATGCAAAATGTTATCAGAATCGCTTGGTGCCCGTTCTTAATAAAAAGCATGCTGTTCAGCTCACAAAATTTGATTACAGACTTTCAAATAAGTTGGATTCAAATTTACAAAAGCTGAGATGTAGAGCCAATTATCAAGCCTTGAAATTTACCGACACGATTAGCAAAATGGGTAAAAAGTTGGTTGAGAGAATGAGCATGAAAAGCAAGCATTTCATTACCCTGCATTTGAGGTTTGAGCCGGATATGCTGGCATTTTCTGGATGCGATTATGGTGGGGGAGAAAAAGAAAGGAAAGAGCTTGGCAAACTTCGGAAAAGGTGGAAAACTTTACATGCAAGCAACCCTGACAAGGTAAGAAGACATGGCAGATGCCCGCTAACTCCAGAGGAAGTTGGTCTCATGCTTAGAGCGTTGGGTTTCGGAAGTGATGTTCACTTGTATGTGGCATCCGGTGAAGTTTATGGAAGTGAAGAGACATTGGCACCCCTAAAAACACTTTTCCCAAACTTCCACACAAAAGAGACTCTGTTAAGCAAGGAGGAGTTGGCCCCATTTTCATCCTTCTCTTCTCGAATGGCTGCACTGGACTTTATTGTTTGCGATGAAAGTGATGTTTTCGTCACCAACAACAATGGCAATATGGCTAAAATGTTAGCTGGTCGAAGGAGATACTTTGGTCACAAACCAACAATCCGTCCAAATGCTAAGAAACTGTCTCAGTTGTTCATAAATCGTAATAACATGACTTGGGAAGAATTTGCATCGAGGATTCGAACAAATCAAATTGGATTCTTGGGAGAGCCAAATGAGGTAAAGCCAGGAAGGGGTGTGTTCCACGAAAACCCCGCTGCCTGCATTTGTAGCACATCTGACACAAAGGCTAAGGAGGATTATACGAACAAGGACAGTGGTTATGCGACAGATGAGCAAGTAACCGAGGACGAGCAAGATTGGTCTGAGATGGATTATGCTGAAATTACAAATCAGTCTCTGGGGAAAGTGCTGCCTAGTGGAAGAGTTTCAGGTCCAAGTGATCTACTCAAATCCGACCAACCAGAACTCGAAGAGTTCTTTTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

605

Amino Acids

67.99

Weight (kDa)

9.17

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 154 - 468 2.7e-101 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 757, 1213
AccI GTMKAC 1 cut(s) 621
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 5 cut(s) 382, 797, 832, 875, 1487
AcvI CACGTG 1 cut(s) 151
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 1 cut(s) 376
AfiI CCNNNNNNNGG 5 cut(s) 274, 344, 680, 1081, 1556
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 5 cut(s) 490, 541, 816, 831, 874
AjnI CCWGG 1 cut(s) 1549
AloI GAACNNNNNNTCC 2 cut(s) 1146, 1178
AluBI AGCT 8 cut(s) 391, 530, 565, 670, 790, 844, 1030, 1388
AluI AGCT 8 cut(s) 391, 530, 565, 670, 790, 844, 1030, 1388
Alw26I GTCTC 8 cut(s) 24, 228, 311, 1133, 1199, 1247, 1452, 1725
AlwNI CAGNNNCTG 2 cut(s) 518, 1723
AoxI GGCC 2 cut(s) 43, 1278
ApeKI GCWGC 7 cut(s) 51, 200, 405, 527, 1310, 1580, 1735
ApoI RAATTY 5 cut(s) 382, 797, 832, 875, 1487
Asp700I GAANNNNTTC 1 cut(s) 1750
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 4 cut(s) 298, 568, 1279, 1757
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 4 cut(s) 239, 257, 1196, 1345
AsuII TTCGAA 2 cut(s) 452, 1504
AvaII GGWCC 3 cut(s) 298, 568, 1757
BaeGI GKGCMC 1 cut(s) 762
BaeI ACNNNNGTAYC 2 cut(s) 1393, 1426
BanI GGYRCC 2 cut(s) 757, 1213
BanII GRGCYC 1 cut(s) 701
BbrPI CACGTG 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 1096
BbvI GCAGC 7 cut(s) 38, 187, 417, 539, 1297, 1567, 1722
BccI CCATC 1 cut(s) 1687
BceAI ACGGC 3 cut(s) 58, 120, 155
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 1 cut(s) 1551
BciVI GTATCC 1 cut(s) 184
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 8 cut(s) 24, 228, 311, 1133, 1199, 1247, 1452, 1725
BfaI CTAG 1 cut(s) 1740
BfuI GTATCC 1 cut(s) 184
Bme1390I CCNGG 2 cut(s) 302, 1551
Bme18I GGWCC 3 cut(s) 298, 568, 1757
BmgT120I GGNCC 4 cut(s) 298, 568, 1279, 1757
BmiI GGNNCC 3 cut(s) 759, 1215, 1281
BmrFI CCNGG 2 cut(s) 302, 1551
BmsI GCATC 7 cut(s) 63, 427, 505, 983, 1091, 1187, 1502
BpiI GAAGAC 1 cut(s) 1096
BpmI CTGGAG 1 cut(s) 1098
Bpu10I CCTNAGC 1 cut(s) 1614
Bpu14I TTCGAA 2 cut(s) 452, 1504
BpuMI CCSGG 1 cut(s) 302
BsaAI YACGTR 1 cut(s) 151
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 1133
BsaJI CCNNGG 3 cut(s) 273, 719, 1668
BsaWI WCCGGW 3 cut(s) 176, 226, 1181
Bsc4I CCNNNNNNNGG 5 cut(s) 274, 344, 680, 1081, 1556
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 3 cut(s) 594, 629, 1320
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 1551
BseDI CCNNGG 3 cut(s) 273, 719, 1668
BseGI GGATG 4 cut(s) 319, 998, 1178, 1289
BseLI CCNNNNNNNGG 5 cut(s) 274, 344, 680, 1081, 1556
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 3 cut(s) 836, 1463, 1680
BseNI ACTGG 3 cut(s) 594, 629, 1320
BseRI GAGGAG 3 cut(s) 203, 262, 1286
BseSI GKGCMC 1 cut(s) 762
BseXI GCAGC 7 cut(s) 38, 187, 417, 539, 1297, 1567, 1722
BsgI GTGCAG 1 cut(s) 1296
BshFI GGCC 2 cut(s) 45, 1280
BshNI GGYRCC 2 cut(s) 757, 1213
BsiSI CCGG 7 cut(s) 8, 177, 227, 301, 338, 971, 1182
BslI CCNNNNNNNGG 5 cut(s) 274, 344, 680, 1081, 1556
BsmAI GTCTC 8 cut(s) 24, 228, 311, 1133, 1199, 1247, 1452, 1725
BsmBI CGTCTC 3 cut(s) 24, 228, 311
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 2 cut(s) 45, 1280
Bso31I GGTCTC 1 cut(s) 1133
Bsp119I TTCGAA 2 cut(s) 452, 1504
Bsp1286I GDGCHC 2 cut(s) 701, 762
Bsp143I GATC 1 cut(s) 1765
BspANI GGCC 2 cut(s) 45, 1280
BspCNI CTCAG 3 cut(s) 837, 1462, 1681
BspLI GGNNCC 3 cut(s) 759, 1215, 1281
BspT104I TTCGAA 2 cut(s) 452, 1504
BspT107I GGYRCC 2 cut(s) 757, 1213
BspTNI GGTCTC 1 cut(s) 1133
BsrDI GCAATG 1 cut(s) 415
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 3 cut(s) 594, 629, 1320
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 3 cut(s) 273, 719, 1668
BssMI GATC 1 cut(s) 1765
BssNI GRCGYC 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 1551
Bst4CI ACNGT 2 cut(s) 1446, 1640
Bst6I CTCTTC 5 cut(s) 20, 1197, 1303, 1741, 1791
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 151
BstBI TTCGAA 2 cut(s) 452, 1504
BstC8I GCNNGC 7 cut(s) 185, 780, 938, 981, 1069, 1106, 1585
BstDEI CTNAG 6 cut(s) 845, 1136, 1437, 1449, 1614, 1689
BstF5I GGATG 4 cut(s) 319, 998, 1178, 1289
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 1 cut(s) 1768
BstMAI GTCTC 8 cut(s) 24, 228, 311, 1133, 1199, 1247, 1452, 1725
BstMBI GATC 1 cut(s) 1765
BstMWI GCNNNNNNNGC 7 cut(s) 51, 83, 141, 524, 933, 976, 1593
BstNI CCWGG 1 cut(s) 1551
BstNSI RCATGY 2 cut(s) 782, 1067
BstSCI CCNGG 2 cut(s) 300, 1549
BstSLI GKGCMC 1 cut(s) 762
BstV1I GCAGC 7 cut(s) 38, 187, 417, 539, 1297, 1567, 1722
BstV2I GAAGAC 1 cut(s) 1096
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 2 cut(s) 45, 1280
BtgZI GCGATG 3 cut(s) 26, 306, 1347
BtsCI GGATG 4 cut(s) 319, 998, 1178, 1289
BtsIMutI CAGTG 2 cut(s) 1313, 1645
Cac8I GCNNGC 7 cut(s) 185, 780, 938, 981, 1069, 1106, 1585
CaiI CAGNNNCTG 2 cut(s) 518, 1723
CfoI GCGC 1 cut(s) 170
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 4 cut(s) 298, 568, 1279, 1757
CseI GACGC 3 cut(s) 41, 85, 251
Csp6I GTAC 1 cut(s) 375
CspCI CAANNNNNGTGG 2 cut(s) 1621, 1656
CviAII CATG 7 cut(s) 709, 779, 928, 1064, 1094, 1132, 1474
CviQI GTAC 1 cut(s) 375
DdeI CTNAG 6 cut(s) 845, 1136, 1437, 1449, 1614, 1689
DpnI GATC 1 cut(s) 1767
DpnII GATC 1 cut(s) 1765
DraIII CACNNNGTG 1 cut(s) 151
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 5 cut(s) 20, 1197, 1303, 1741, 1791
EarI CTCTTC 5 cut(s) 20, 1197, 1303, 1741, 1791
EciI GGCGGA 2 cut(s) 122, 131
Eco24I GRGCYC 1 cut(s) 701
Eco31I GGTCTC 1 cut(s) 1133
Eco47I GGWCC 3 cut(s) 298, 568, 1757
Eco72I CACGTG 1 cut(s) 151
EcoRI GAATTC 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 1549
EcoT38I GRGCYC 1 cut(s) 701
Esp3I CGTCTC 3 cut(s) 24, 228, 311
FaeI CATG 7 cut(s) 712, 782, 931, 1067, 1097, 1135, 1477
FatI CATG 7 cut(s) 708, 778, 927, 1063, 1093, 1131, 1473
FauI CCCGC 3 cut(s) 165, 1113, 1585
FblI GTMKAC 1 cut(s) 621
FokI GGATG 4 cut(s) 326, 1005, 1165, 1276
FriOI GRGCYC 1 cut(s) 701
FspBI CTAG 1 cut(s) 1740
GlaI GCGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 1098
HaeIII GGCC 2 cut(s) 45, 1280
HapII CCGG 7 cut(s) 8, 177, 227, 301, 338, 971, 1182
HgaI GACGC 3 cut(s) 41, 85, 251
HhaI GCGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 7 cut(s) 712, 782, 931, 1067, 1097, 1135, 1477
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 7 cut(s) 454, 590, 748, 827, 1255, 1501, 1521
HpaII CCGG 7 cut(s) 8, 177, 227, 301, 338, 971, 1182
HphI GGTGA 4 cut(s) 239, 257, 1196, 1345
Hpy166II GTNNAC 3 cut(s) 280, 622, 1165
Hpy188III TCNNGA 5 cut(s) 362, 538, 990, 1115, 1302
Hpy8I GTNNAC 3 cut(s) 280, 622, 1165
Hpy99I CGWCG 3 cut(s) 71, 325, 328
HpyAV CCTTC 5 cut(s) 214, 580, 1303, 1389, 1548
HpyCH4III ACNGT 2 cut(s) 1446, 1640
HpyCH4IV ACGT 4 cut(s) 69, 150, 326, 618
HpyF10VI GCNNNNNNNGC 7 cut(s) 51, 83, 141, 524, 933, 976, 1593
HpyF3I CTNAG 6 cut(s) 845, 1136, 1437, 1449, 1614, 1689
HpySE526I ACGT 4 cut(s) 69, 150, 326, 618
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 7 cut(s) 712, 782, 931, 1067, 1097, 1135, 1477
HspAI GCGC 1 cut(s) 168
Kzo9I GATC 1 cut(s) 1765
LmnI GCTCC 2 cut(s) 223, 388
Lsp1109I GCAGC 7 cut(s) 38, 187, 417, 539, 1297, 1567, 1722
LweI GCATC 7 cut(s) 63, 427, 505, 983, 1091, 1187, 1502
MaeI CTAG 1 cut(s) 1740
MaeII ACGT 4 cut(s) 69, 150, 326, 618
MaeIII GTNAC 6 cut(s) 3, 263, 596, 1351, 1409, 1663
MalI GATC 1 cut(s) 1767
MboI GATC 1 cut(s) 1765
MhlI GDGCHC 2 cut(s) 701, 762
MluCI AATT 9 cut(s) 307, 382, 797, 832, 859, 875, 1487, 1515, 1707
MlyI GAGTC 1 cut(s) 1249
MmeI TCCRAC 3 cut(s) 698, 804, 1803
MroXI GAANNNNTTC 1 cut(s) 1750
MseI TTAA 4 cut(s) 63, 654, 768, 1262
MslI CAYNNNNRTG 1 cut(s) 1170
MspA1I CMGCKG 2 cut(s) 51, 1580
MspI CCGG 7 cut(s) 8, 177, 227, 301, 338, 971, 1182
MspR9I CCNGG 2 cut(s) 302, 1551
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 1 cut(s) 1551
MwoI GCNNNNNNNGC 7 cut(s) 51, 83, 141, 524, 933, 976, 1593
NciI CCSGG 1 cut(s) 302
NdeII GATC 1 cut(s) 1765
NlaIII CATG 7 cut(s) 712, 782, 931, 1067, 1097, 1135, 1477
NlaIV GGNNCC 3 cut(s) 759, 1215, 1281
NmuCI GTSAC 3 cut(s) 263, 1351, 1409
NspI RCATGY 2 cut(s) 782, 1067
NspV TTCGAA 2 cut(s) 452, 1504
PaeI GCATGC 1 cut(s) 782
PcsI WCGNNNNNNNCGW 1 cut(s) 317
PctI GAATGC 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 1750
PfeI GAWTC 6 cut(s) 454, 590, 748, 827, 1501, 1521
PfoI TCCNGGA 1 cut(s) 300
PleI GAGTC 1 cut(s) 1249
PmaCI CACGTG 1 cut(s) 151
PmlI CACGTG 1 cut(s) 151
PpsI GAGTC 1 cut(s) 1249
Ppu21I YACGTR 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 1549
PspCI CACGTG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 1549
PspN4I GGNNCC 3 cut(s) 759, 1215, 1281
PspPI GGNCC 4 cut(s) 298, 568, 1279, 1757
PstNI CAGNNNCTG 2 cut(s) 518, 1723
RsaI GTAC 1 cut(s) 376
RsaNI GTAC 1 cut(s) 375
RseI CAYNNNNRTG 1 cut(s) 1170
SaqAI TTAA 4 cut(s) 63, 654, 768, 1262
Sau3AI GATC 1 cut(s) 1765
Sau96I GGNCC 4 cut(s) 298, 568, 1279, 1757
SchI GAGTC 1 cut(s) 1249
ScrFI CCNGG 2 cut(s) 302, 1551
SduI GDGCHC 2 cut(s) 701, 762
SfaNI GCATC 7 cut(s) 63, 427, 505, 983, 1091, 1187, 1502
SfuI TTCGAA 2 cut(s) 452, 1504
SinI GGWCC 3 cut(s) 298, 568, 1757
SmiMI CAYNNNNRTG 1 cut(s) 1170
SphI GCATGC 1 cut(s) 782
Sse9I AATT 9 cut(s) 307, 382, 797, 832, 859, 875, 1487, 1515, 1707
SspMI CTAG 1 cut(s) 1740
StyD4I CCNGG 2 cut(s) 300, 1549
TaaI ACNGT 2 cut(s) 1446, 1640
TaiI ACGT 4 cut(s) 72, 153, 329, 621
TaqI TCGA 7 cut(s) 361, 452, 1303, 1393, 1496, 1504, 1794
TasI AATT 9 cut(s) 307, 382, 797, 832, 859, 875, 1487, 1515, 1707
TatI WGTACW 1 cut(s) 374
TauI GCSGC 6 cut(s) 80, 83, 86, 96, 291, 416
TfiI GAWTC 6 cut(s) 454, 590, 748, 827, 1501, 1521
Tru1I TTAA 4 cut(s) 63, 654, 768, 1262
Tru9I TTAA 4 cut(s) 63, 654, 768, 1262
TscAI CASTG 2 cut(s) 1320, 1645
TseFI GTSAC 3 cut(s) 263, 1351, 1409
TseI GCWGC 7 cut(s) 51, 200, 405, 527, 1310, 1580, 1735
Tsp45I GTSAC 3 cut(s) 263, 1351, 1409
TspDTI ATGAA 7 cut(s) 29, 621, 934, 944, 1278, 1350, 1447
TspGWI ACGGA 2 cut(s) 210, 1415
TspRI CASTG 2 cut(s) 1320, 1645
VpaK11BI GGWCC 3 cut(s) 298, 568, 1757
XapI RAATTY 5 cut(s) 382, 797, 832, 875, 1487
XceI RCATGY 2 cut(s) 782, 1067
XmiI GTMKAC 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 1750
XspI CTAG 1 cut(s) 1740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.