Rroxscaffold_7G00188360

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010779
Physical Location & Seq
Forward (+)
27941983 .. 27946110
4128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00188360.1

Sequence Viewer

Length: 1833 bp
ATGTCTATGTTCTCTCCAATGAAGAAGAAGAAGAGAGACAAGTCGGACAACAACAACCACCTCAACGGCGTCGGTCTCCACAGCCCCATTCTCCGGCGGCACATCCGCCGCTACCGCCACCACATCTTCCCGGTAATCTCCGTCGTCTCCGGCTGCCTGCTTCTCTTCCTCGTCGCGTTCTCTCTCGTCGCTCGTTCGCCGCCTCTCCACCACCACGATCACCTCTCGCTCCTCCGTTTCCACGCCTCCTCGGCTGATAACGGCGTCGTCGAGGTCCAGTTGAACAATGACGAGACGCCGGCGTTTCGTGTGCCGGAGAGTGGAGGGAGCTCAGTCCAACATTTATGGAGCGGAGCGCAATCGGAGCTGTTCTTTGGTTGTAGCAACGCCGGCGACAAGTTTTTGACAGCCGATATGAAAACGCTTCCCAATCGGTACTTGTTGATTGCTACCAGTGGAGGCTTGAATCAACAGAGAACAGGGATAACAGATGCTGTTGTTGCAGCTTATATTTTAAATGCTACTCTTGTTGTTCCGAAGCTGGACCATCAATCATTTTGGAAGGATTCCAGCAACTTTGCTGAGATCTTTGATGTAGACTGGTTCATATCTTTTCTGTCAAATGATGTCAAAATCATTAAAAAGCTTCCATCGAAGGGAGGGACACCAATGAGTCCATATACCATGCGTGTCCCGAGGAAGTGTAATGTGAAATGCTACCTGAATCGCGTAGTGCCTGTTCTTAATAAAAAGCATGCTGTTCAGCTCACTAAGTTTGATTACAGGCTTTCAAATAACCTGGAATCACATTTACAGAAGCTGAGATGTAGGACTAATTATCACGCCTTGAAATTTACTGACAATATTAATGAAATGGGGAGGAAGTTGGTTGAGAGAATGAAGATGAAAAACAAGCATTTCATTGCCCTGCATTTGAGGTTTGAGCCGGATATGCTGGCATTTTCTGGTTGTTATTTTGGTGGGGGAGAAAAAGAAAGGAATGAACTTGGTGCAATTCGGAAAAGGTGGAAAACTCTACATCCAACCAACCCTGACAAGGTGCGAAGACGTGGTAGATGCCCTCTAAGCCCAGGAGAAGTTGGTCTTATGCTTAGAGCATTGGGCTTCGGAAGTGATGTTCACTTGTATGTGGCATCAGGTGAAGTATATAAAGGTGAAGAGACATTGGCACCGCTGAAAGCACTTTTCCCAAACTTCCATACAAAGGAGACTATAGCAAGTAAGGAAGAGTTGGAACCATTTTCTTCCTTCTCTTCTCGCATGGCTGCACTAGATTTCATTGTCTGTGATGAAAGTGATGTCTTCGTCACCAACAATAATGGCAACATGGCTAGAATATTAGCTGGTCGAAGGAGATACTTTGGGCATAAACCAACAATCCGTCCAAACACTAAGAAACTGTACAAGTTGTTCATTAACCAAAATAACATGACATGGGAGGAATTTGCTTCCAGGGTTCGAACTAGTCAAATTGGATCCATGGGAGAGCCAAATGAGAAAAAGCCAGGCAAGGGTGAGTTCCATGAAAATCCAGCAGACTGCATCTGTAACACATCTAATACAAAGTCCATAGACGTCCGAATTCCTCAAATTGAAATTAATGAAGGCACTAACATCAGTAAGGAAGATGAGACAAGTAAGGATAGTGGTTATGCGACAGATGGGCACATGACGGATGACGAGCAATTTTGGCCTGAGATGGATTATGTGGATACTGGAAACAAGTCTCTGGGAAAAGGGCCTAGTCAAAGATTTTCGTATCAGGGTCTTAATTATGATCTGCCAGAACTTCTAGAGCTCTTTTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

610

Amino Acids

69.14

Weight (kDa)

9.18

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 146 - 460 3.5e-101 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1599
AccB1I GGYRCC 1 cut(s) 1189
AccBSI CCGCTC 1 cut(s) 351
AccI GTMKAC 1 cut(s) 597
AccII CGCG 2 cut(s) 176, 729
AciI CCGC 7 cut(s) 97, 106, 109, 115, 200, 351, 1193
AclWI GGATC 2 cut(s) 1491, 1504
AcsI RAATTY 3 cut(s) 851, 1463, 1602
AcyI GRCGYC 4 cut(s) 69, 264, 296, 1596
AfaI GTAC 2 cut(s) 437, 1424
AfiI CCNNNNNNNGG 6 cut(s) 93, 320, 656, 1057, 1225, 1532
AgsI TTSAA 5 cut(s) 283, 466, 792, 850, 1616
AhlI ACTAGT 1 cut(s) 1484
AjiI CACGTC 1 cut(s) 1070
AjnI CCWGG 4 cut(s) 798, 1090, 1472, 1525
AloI GAACNNNNNNTCC 2 cut(s) 1122, 1154
AluBI AGCT 9 cut(s) 330, 367, 506, 541, 646, 766, 820, 1364, 1819
AluI AGCT 9 cut(s) 330, 367, 506, 541, 646, 766, 820, 1364, 1819
Alw21I GWGCWC 2 cut(s) 332, 1821
Alw26I GTCTC 8 cut(s) 30, 80, 151, 287, 1175, 1223, 1646, 1752
AlwI GGATC 2 cut(s) 1491, 1504
AlwNI CAGNNNCTG 2 cut(s) 494, 820
Ama87I CYCGRG 1 cut(s) 694
AoxI GGCC 2 cut(s) 1712, 1760
ApeKI GCWGC 3 cut(s) 153, 503, 1286
ApoI RAATTY 3 cut(s) 851, 1463, 1602
AseI ATTAAT 2 cut(s) 867, 1620
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 3 cut(s) 274, 544, 1760
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 5 cut(s) 212, 1172, 1187, 1321, 1547
AsuII TTCGAA 1 cut(s) 1480
AvaI CYCGRG 1 cut(s) 694
AvaII GGWCC 2 cut(s) 274, 544
BaeGI GKGCMC 1 cut(s) 1689
BamHI GGATCC 1 cut(s) 1496
BanI GGYRCC 1 cut(s) 1189
BanII GRGCYC 2 cut(s) 332, 1821
BbsI GAAGAC 2 cut(s) 1072, 1315
Bbv12I GWGCWC 2 cut(s) 332, 1821
BbvI GCAGC 3 cut(s) 140, 515, 1273
BccI CCATC 4 cut(s) 555, 658, 1676, 1714
BceAI ACGGC 2 cut(s) 82, 277
BciT130I CCWGG 4 cut(s) 800, 1092, 1474, 1527
BciVI GTATCC 1 cut(s) 1726
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 8 cut(s) 30, 80, 151, 287, 1175, 1223, 1646, 1752
BcuI ACTAGT 1 cut(s) 1484
BfaI CTAG 5 cut(s) 1292, 1353, 1485, 1764, 1814
BfmI CTRYAG 1 cut(s) 1233
BfuI GTATCC 1 cut(s) 1726
BglI GCCNNNNNGGC 1 cut(s) 251
BglII AGATCT 1 cut(s) 585
BisI GCNGC 6 cut(s) 98, 109, 154, 200, 504, 1287
BlsI GCNGC 6 cut(s) 99, 110, 155, 201, 505, 1288
Bme1390I CCNGG 5 cut(s) 131, 800, 1092, 1474, 1527
Bme18I GGWCC 2 cut(s) 274, 544
BmeT110I CYCGRG 1 cut(s) 694
BmgBI CACGTC 1 cut(s) 1070
BmgT120I GGNCC 3 cut(s) 274, 544, 1760
BmiI GGNNCC 3 cut(s) 1191, 1257, 1498
BmrFI CCNGG 5 cut(s) 131, 800, 1092, 1474, 1527
BmsI GCATC 4 cut(s) 481, 1067, 1163, 1572
BpiI GAAGAC 2 cut(s) 1072, 1315
Bpu14I TTCGAA 1 cut(s) 1480
BpuMI CCSGG 1 cut(s) 131
BsaHI GRCGYC 4 cut(s) 69, 264, 296, 1596
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 5 cut(s) 249, 695, 1090, 1473, 1500
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 320, 656, 1057, 1225, 1532
Bse118I RCCGGY 2 cut(s) 298, 389
Bse1I ACTGG 4 cut(s) 277, 453, 605, 1741
Bse3DI GCAATG 1 cut(s) 921
BseBI CCWGG 4 cut(s) 800, 1092, 1474, 1527
BseDI CCNNGG 5 cut(s) 249, 695, 1090, 1473, 1500
BseGI GGATG 3 cut(s) 102, 1039, 1702
BseLI CCNNNNNNNGG 6 cut(s) 93, 320, 656, 1057, 1225, 1532
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 4 cut(s) 345, 573, 812, 1707
BseNI ACTGG 4 cut(s) 277, 453, 605, 1741
BseRI GAGGAG 2 cut(s) 221, 238
BseSI GKGCMC 1 cut(s) 1689
BseXI GCAGC 3 cut(s) 140, 515, 1273
BsgI GTGCAG 1 cut(s) 1272
Bsh1236I CGCG 2 cut(s) 176, 729
BshFI GGCC 2 cut(s) 1714, 1762
BshNI GGYRCC 1 cut(s) 1189
BsiHKAI GWGCWC 2 cut(s) 332, 1821
BsiHKCI CYCGRG 1 cut(s) 694
BsiSI CCGG 7 cut(s) 94, 131, 150, 299, 314, 390, 947
BslFI GGGAC 2 cut(s) 676, 677
BslI CCNNNNNNNGG 6 cut(s) 93, 320, 656, 1057, 1225, 1532
BsmAI GTCTC 8 cut(s) 30, 80, 151, 287, 1175, 1223, 1646, 1752
BsmBI CGTCTC 2 cut(s) 151, 287
BsmFI GGGAC 2 cut(s) 676, 677
BsnI GGCC 2 cut(s) 1714, 1762
Bso31I GGTCTC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 694
Bsp119I TTCGAA 1 cut(s) 1480
Bsp1286I GDGCHC 3 cut(s) 332, 1689, 1821
Bsp1407I TGTACA 1 cut(s) 1422
Bsp143I GATC 4 cut(s) 217, 585, 1496, 1798
Bsp19I CCATGG 1 cut(s) 1500
BspACI CCGC 7 cut(s) 97, 106, 109, 115, 200, 351, 1193
BspANI GGCC 2 cut(s) 1714, 1762
BspCNI CTCAG 4 cut(s) 344, 574, 813, 1708
BspFNI CGCG 2 cut(s) 176, 729
BspLI GGNNCC 3 cut(s) 1191, 1257, 1498
BspPI GGATC 2 cut(s) 1491, 1504
BspT104I TTCGAA 1 cut(s) 1480
BspT107I GGYRCC 1 cut(s) 1189
BspTNI GGTCTC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 351
BsrDI GCAATG 1 cut(s) 921
BsrFI RCCGGY 2 cut(s) 298, 389
BsrGI TGTACA 1 cut(s) 1422
BsrI ACTGG 4 cut(s) 277, 453, 605, 1741
BssAI RCCGGY 2 cut(s) 298, 389
BssECI CCNNGG 5 cut(s) 249, 695, 1090, 1473, 1500
BssMI GATC 4 cut(s) 217, 585, 1496, 1798
BssNI GRCGYC 4 cut(s) 69, 264, 296, 1596
BssT1I CCWWGG 1 cut(s) 1500
Bst2UI CCWGG 4 cut(s) 800, 1092, 1474, 1527
Bst4CI ACNGT 1 cut(s) 1422
Bst6I CTCTTC 5 cut(s) 26, 170, 1173, 1242, 1279
BstACI GRCGYC 4 cut(s) 69, 264, 296, 1596
BstAUI TGTACA 1 cut(s) 1422
BstBI TTCGAA 1 cut(s) 1480
BstC8I GCNNGC 5 cut(s) 158, 300, 391, 756, 957
BstDEI CTNAG 8 cut(s) 331, 582, 771, 821, 1085, 1112, 1413, 1716
BstDSI CCRYGG 1 cut(s) 1500
BstF5I GGATG 3 cut(s) 102, 1039, 1702
BstFNI CGCG 2 cut(s) 176, 729
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 4 cut(s) 220, 588, 1499, 1801
BstMAI GTCTC 8 cut(s) 30, 80, 151, 287, 1175, 1223, 1646, 1752
BstMBI GATC 4 cut(s) 217, 585, 1496, 1798
BstMWI GCNNNNNNNGC 8 cut(s) 114, 251, 364, 390, 500, 952, 1086, 1711
BstNI CCWGG 4 cut(s) 800, 1092, 1474, 1527
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 5 cut(s) 129, 798, 1090, 1472, 1525
BstSFI CTRYAG 1 cut(s) 1233
BstSLI GKGCMC 1 cut(s) 1689
BstUI CGCG 2 cut(s) 176, 729
BstV1I GCAGC 3 cut(s) 140, 515, 1273
BstV2I GAAGAC 2 cut(s) 1072, 1315
BstX2I RGATCY 2 cut(s) 585, 1496
BstYI RGATCY 2 cut(s) 585, 1496
BsuI GTATCC 1 cut(s) 1726
BsuRI GGCC 2 cut(s) 1714, 1762
BtgI CCRYGG 1 cut(s) 1500
BtrI CACGTC 1 cut(s) 1070
BtsCI GGATG 3 cut(s) 102, 1039, 1702
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 5 cut(s) 158, 300, 391, 756, 957
CaiI CAGNNNCTG 2 cut(s) 494, 820
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 2 cut(s) 298, 389
Cfr13I GGNCC 3 cut(s) 274, 544, 1760
CseI GACGC 3 cut(s) 58, 253, 304
Csp6I GTAC 2 cut(s) 436, 1423
CviAII CATG 9 cut(s) 685, 755, 1282, 1348, 1450, 1455, 1501, 1544, 1690
CviQI GTAC 2 cut(s) 436, 1423
DdeI CTNAG 8 cut(s) 331, 582, 771, 821, 1085, 1112, 1413, 1716
DpnI GATC 4 cut(s) 219, 587, 1498, 1800
DpnII GATC 4 cut(s) 217, 585, 1496, 1798
DraI TTTAAA 1 cut(s) 516
Eam1104I CTCTTC 5 cut(s) 26, 170, 1173, 1242, 1279
EarI CTCTTC 5 cut(s) 26, 170, 1173, 1242, 1279
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 2 cut(s) 330, 1819
Eco130I CCWWGG 1 cut(s) 1500
Eco24I GRGCYC 2 cut(s) 332, 1821
Eco31I GGTCTC 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 274, 544
Eco53kI GAGCTC 2 cut(s) 330, 1819
Eco88I CYCGRG 1 cut(s) 694
EcoICRI GAGCTC 2 cut(s) 330, 1819
EcoO109I RGGNCCY 1 cut(s) 1760
EcoRI GAATTC 1 cut(s) 1602
EcoRII CCWGG 4 cut(s) 798, 1090, 1472, 1525
EcoT14I CCWWGG 1 cut(s) 1500
EcoT38I GRGCYC 2 cut(s) 332, 1821
ErhI CCWWGG 1 cut(s) 1500
Esp3I CGTCTC 2 cut(s) 151, 287
FaeI CATG 9 cut(s) 688, 758, 1285, 1351, 1453, 1458, 1504, 1547, 1693
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 2 cut(s) 676, 677
FatI CATG 9 cut(s) 684, 754, 1281, 1347, 1449, 1454, 1500, 1543, 1689
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 6 cut(s) 98, 109, 154, 200, 504, 1287
FokI GGATG 3 cut(s) 89, 1026, 1709
FriOI GRGCYC 2 cut(s) 332, 1821
Fsp4HI GCNGC 6 cut(s) 98, 109, 154, 200, 504, 1287
FspBI CTAG 5 cut(s) 1292, 1353, 1485, 1764, 1814
GlaI GCGC 1 cut(s) 357
GluI GCNGC 6 cut(s) 98, 109, 154, 200, 504, 1287
HaeIII GGCC 2 cut(s) 1714, 1762
HapII CCGG 7 cut(s) 94, 131, 150, 299, 314, 390, 947
HgaI GACGC 3 cut(s) 58, 253, 304
HhaI GCGC 1 cut(s) 358
Hin1I GRCGYC 4 cut(s) 69, 264, 296, 1596
Hin1II CATG 9 cut(s) 688, 758, 1285, 1351, 1453, 1458, 1504, 1547, 1693
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 5 cut(s) 466, 566, 673, 724, 803
HpaII CCGG 7 cut(s) 94, 131, 150, 299, 314, 390, 947
HphI GGTGA 5 cut(s) 212, 1172, 1187, 1321, 1547
Hpy166II GTNNAC 2 cut(s) 598, 1141
Hpy188I TCNGA 7 cut(s) 46, 364, 537, 1020, 1130, 1601, 1828
Hpy188III TCNNGA 2 cut(s) 694, 1814
Hpy8I GTNNAC 2 cut(s) 598, 1141
Hpy99I CGWCG 6 cut(s) 74, 146, 176, 191, 269, 272
HpyAV CCTTC 5 cut(s) 556, 649, 1279, 1365, 1619
HpyCH4III ACNGT 1 cut(s) 1422
HpyCH4IV ACGT 2 cut(s) 1069, 1596
HpyCH4V TGCA 5 cut(s) 503, 931, 1013, 1289, 1563
HpyF10VI GCNNNNNNNGC 8 cut(s) 114, 251, 364, 390, 500, 952, 1086, 1711
HpyF3I CTNAG 8 cut(s) 331, 582, 771, 821, 1085, 1112, 1413, 1716
HpySE526I ACGT 2 cut(s) 1069, 1596
Hsp92I GRCGYC 4 cut(s) 69, 264, 296, 1596
Hsp92II CATG 9 cut(s) 688, 758, 1285, 1351, 1453, 1458, 1504, 1547, 1693
HspAI GCGC 1 cut(s) 356
KroI GCCGGC 2 cut(s) 298, 389
KroNI GCCGGC 2 cut(s) 300, 391
Kzo9I GATC 4 cut(s) 217, 585, 1496, 1798
LmnI GCTCC 5 cut(s) 234, 327, 348, 353, 364
Lsp1109I GCAGC 3 cut(s) 140, 515, 1273
LweI GCATC 4 cut(s) 481, 1067, 1163, 1572
MaeI CTAG 5 cut(s) 1292, 1353, 1485, 1764, 1814
MaeII ACGT 2 cut(s) 1069, 1596
MaeIII GTNAC 2 cut(s) 1327, 1568
MalI GATC 4 cut(s) 219, 587, 1498, 1800
MbiI CCGCTC 1 cut(s) 351
MboI GATC 4 cut(s) 217, 585, 1496, 1798
MflI RGATCY 2 cut(s) 585, 1496
MhlI GDGCHC 3 cut(s) 332, 1689, 1821
MlyI GAGTC 1 cut(s) 682
MmeI TCCRAC 4 cut(s) 24, 361, 1067, 1233
MreI CGCCGGCG 2 cut(s) 298, 389
MroNI GCCGGC 2 cut(s) 298, 389
MseI TTAA 7 cut(s) 515, 639, 744, 867, 1437, 1620, 1791
MslI CAYNNNNRTG 1 cut(s) 1146
MspA1I CMGCKG 1 cut(s) 1195
MspI CCGG 7 cut(s) 94, 131, 150, 299, 314, 390, 947
MspR9I CCNGG 5 cut(s) 131, 800, 1092, 1474, 1527
MvaI CCWGG 4 cut(s) 800, 1092, 1474, 1527
MvnI CGCG 2 cut(s) 176, 729
MwoI GCNNNNNNNGC 8 cut(s) 114, 251, 364, 390, 500, 952, 1086, 1711
NaeI GCCGGC 2 cut(s) 300, 391
NciI CCSGG 1 cut(s) 131
NcoI CCATGG 1 cut(s) 1500
NdeII GATC 4 cut(s) 217, 585, 1496, 1798
NgoMIV GCCGGC 2 cut(s) 298, 389
NlaIII CATG 9 cut(s) 688, 758, 1285, 1351, 1453, 1458, 1504, 1547, 1693
NlaIV GGNNCC 3 cut(s) 1191, 1257, 1498
NmeAIII GCCGAG 1 cut(s) 230
NmuCI GTSAC 1 cut(s) 1327
NspI RCATGY 1 cut(s) 758
NspV TTCGAA 1 cut(s) 1480
PaeI GCATGC 1 cut(s) 758
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 2 cut(s) 300, 391
PfeI GAWTC 4 cut(s) 466, 566, 724, 803
PkrI GCNGC 6 cut(s) 99, 110, 155, 201, 505, 1288
PleI GAGTC 1 cut(s) 681
PpsI GAGTC 1 cut(s) 681
PshBI ATTAAT 2 cut(s) 867, 1620
Psp124BI GAGCTC 2 cut(s) 332, 1821
Psp6I CCWGG 4 cut(s) 798, 1090, 1472, 1525
PspGI CCWGG 4 cut(s) 798, 1090, 1472, 1525
PspN4I GGNNCC 3 cut(s) 1191, 1257, 1498
PspPI GGNCC 3 cut(s) 274, 544, 1760
PsrI GAACNNNNNNTAC 4 cut(s) 723, 755, 1415, 1447
PstNI CAGNNNCTG 2 cut(s) 494, 820
PsuI RGATCY 2 cut(s) 585, 1496
RsaI GTAC 2 cut(s) 437, 1424
RsaNI GTAC 2 cut(s) 436, 1423
RseI CAYNNNNRTG 1 cut(s) 1146
SacI GAGCTC 2 cut(s) 332, 1821
SaqAI TTAA 7 cut(s) 515, 639, 744, 867, 1437, 1620, 1791
SatI GCNGC 6 cut(s) 98, 109, 154, 200, 504, 1287
Sau3AI GATC 4 cut(s) 217, 585, 1496, 1798
Sau96I GGNCC 3 cut(s) 274, 544, 1760
SchI GAGTC 1 cut(s) 682
ScrFI CCNGG 5 cut(s) 131, 800, 1092, 1474, 1527
SduI GDGCHC 3 cut(s) 332, 1689, 1821
SfaNI GCATC 4 cut(s) 481, 1067, 1163, 1572
SfcI CTRYAG 1 cut(s) 1233
SfuI TTCGAA 1 cut(s) 1480
SgrAI CRCCGGYG 2 cut(s) 298, 389
SinI GGWCC 2 cut(s) 274, 544
SmiMI CAYNNNNRTG 1 cut(s) 1146
SpeI ACTAGT 1 cut(s) 1484
SphI GCATGC 1 cut(s) 758
SsiI CCGC 7 cut(s) 97, 106, 109, 115, 200, 351, 1193
SspI AATATT 2 cut(s) 865, 1359
SspMI CTAG 5 cut(s) 1292, 1353, 1485, 1764, 1814
SstI GAGCTC 2 cut(s) 332, 1821
StyD4I CCNGG 5 cut(s) 129, 798, 1090, 1472, 1525
StyI CCWWGG 1 cut(s) 1500
TaaI ACNGT 1 cut(s) 1422
TaiI ACGT 2 cut(s) 1072, 1599
TaqI TCGA 4 cut(s) 270, 653, 1369, 1480
TaqII GACCGA 1 cut(s) 62
TatI WGTACW 1 cut(s) 1422
TauI GCSGC 3 cut(s) 100, 111, 202
TfiI GAWTC 4 cut(s) 466, 566, 724, 803
Tru1I TTAA 7 cut(s) 515, 639, 744, 867, 1437, 1620, 1791
Tru9I TTAA 7 cut(s) 515, 639, 744, 867, 1437, 1620, 1791
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 1 cut(s) 1327
TseI GCWGC 3 cut(s) 153, 503, 1286
Tsp45I GTSAC 1 cut(s) 1327
TspGWI ACGGA 4 cut(s) 130, 224, 1391, 1709
TspRI CASTG 1 cut(s) 460
VpaK11BI GGWCC 2 cut(s) 274, 544
VspI ATTAAT 2 cut(s) 867, 1620
XapI RAATTY 3 cut(s) 851, 1463, 1602
XbaI TCTAGA 1 cut(s) 1813
XceI RCATGY 1 cut(s) 758
XmiI GTMKAC 1 cut(s) 597
XspI CTAG 5 cut(s) 1292, 1353, 1485, 1764, 1814
ZraI GACGTC 1 cut(s) 1597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.