pycom05g12960

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010779
Physical Location & Seq
Reverse (-)
16500458 .. 16503672
3215 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12960.6

Sequence Viewer

Length: 1782 bp
ATGTCACCGGCGATGAAGAAGAAGAAGAGAGACGCAAACAACGGTCACCGCTGCGTCTATATTAACGACGTTATCACCGCTGCCGCCATAGGCGGCGTCGTTTTCAGAACCTCTATTCTTCGGCGATACCTCCGCCGTCTCCGCCACCACGTGTTCCCGCTAATCTGCGCCGTATCCGGTTGCCTGCTTATCTTCCTCGCTGCCTTCTCTCTCCTCGCTCCGTCTCCGGTTCTGCATTTTCACAATCACCTCTCGCTCTTGCGTCACCTCTCCTCGGTACTCAAGCACACACCGAATCACCTCTCAGAGAGCGGAGGAAGTTCAGGTCGAGATTTATGGAGTACAAGGAATTCGGAGGTTTTCTTCGGTTGCAGCAATGCCGCCGATAACTTTGCAACTGCTGATGCGAAAACTCATTCGAATCGTTACTTGTTGATTGCTACAAGTGGAGGTTTGAACCAACAAAGAACAGGGATAACAGATGCTGTCGTTGCAGCTTATATCTTGAATGCTACTCTTGTTGTTCCCAAGCTGGACCAGAAATCGTATTGGAAGGATTCCAGTAACTTTGATGAAATCTTTGACGTAGACTGGTTTATATCATCTCTATCAAAAGATGTTAAAATCATAAAACAGCTTCCAACAAAGGGAGGGAAACCAATGAGCCCATATACCATGCGAGTTCCGAGGAAGTGTAATGCAAAATGTTATCAGAATCGCTTGGTGCCCGTTCTTAATAAAAAGCATGCTGTTCAGCTCACAAAATTTGATTACAGACTTTCAAATAAGTTGGATTCAAATTTACAAAAACTGAGATGTAGAGCCAATTATCAAGCCTTGAAATTTACCGACACGATTAGAAAAATGGGTAAAAAGTTGGTTGAGAGAATGAGCAAGAAAAACAAGCATTTCATTGCCCTGCATTTGAGGTTTGAGCCGGATATGCTGGCGTTTTCTGGATGCGATTATGGTGGGGGAGAAAAAGAAAGGAAAGAGCTTGGCAAACTTCGGAAAAGGTGGAAAACTTTACATGCAAGCAACCCTGACAAGGTACGAAGACATGGCAGATGCCCGCTAACTCCAGAGGAAGTTGGTCTCATGCTTAGAGCGTTGGGTTTCGGACGTGATGTTCACTTGTATGTGGCATCCGGTGATGTATATGGAGGTGAAGAGACATTGGCACCCCTAAAATCACTTTTCCCAAACTTCCACACAAAAGAGACTCTGTTAAGCAGGGAGGAGTTGGCCCCGTTTTCATCCTTCTCTTCTCGAATGGCTGCGCTGGACTTTATTGTTTGCGATGAAAGTGATGTTTTCGTCACAAACAACAATGGCAACATGGCTAAAATGTTAGCTGGTCGAAGGAGATACTTTGGTCACAAACCAACAATCCGTCCCAATGCTAAGAAACTGTCTCAGTTGTTCATAAATCGTAATAACATGACATGGGAAGAATTTGCATCGAGGATTCGAACAGATCAAATTGGATTCTTGGGAGAGCCAAATGAGGTAAAGCCGGGAAGGGGTGTGTTCCACGAAAACCCCGCTGCCTGCATTTGTAGCACATCTGACACAAAGGCTAAGGAGGATTATATGAACAAGGACAGTGGTTATGCGACAGATGAGCAAGTAACCGAGGACGAGCAAGATTGGTCTGAGATGGATTATGCTGAAATTACAAATCAGTCTATAGGGAAAGTGCTGCCTAGTGGAAGCGTTTCAGGTCCAAGTGTTCTACTTAAATCCGACCAACCAGAATTCGAAGAGTTCTTTTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

594

Amino Acids

66.85

Weight (kDa)

9.43

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 724, 1180
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 588
AcsI RAATTY 6 cut(s) 349, 764, 799, 842, 1454, 1757
AcvI CACGTG 1 cut(s) 151
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 3 cut(s) 279, 343, 1053
AfiI CCNNNNNNNGG 4 cut(s) 274, 647, 1048, 1523
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 5 cut(s) 457, 508, 783, 798, 841
AjiI CACGTC 1 cut(s) 1124
AloI GAACNNNNNNTCC 2 cut(s) 1113, 1145
AluBI AGCT 6 cut(s) 497, 532, 637, 757, 997, 1355
AluI AGCT 6 cut(s) 497, 532, 637, 757, 997, 1355
Alw26I GTCTC 7 cut(s) 24, 143, 228, 1100, 1166, 1214, 1419
AlwNI CAGNNNCTG 1 cut(s) 485
AoxI GGCC 1 cut(s) 1245
ApeKI GCWGC 8 cut(s) 51, 80, 200, 372, 494, 1277, 1547, 1702
ApoI RAATTY 6 cut(s) 349, 764, 799, 842, 1454, 1757
Asp700I GAANNNNTTC 1 cut(s) 1717
AspLEI GCGC 2 cut(s) 170, 1282
AspS9I GGNCC 3 cut(s) 535, 1246, 1724
AsuC2I CCSGG 1 cut(s) 1518
AsuHPI GGTGA 7 cut(s) 38, 67, 239, 257, 290, 1163, 1178
AsuII TTCGAA 3 cut(s) 419, 1471, 1761
AvaII GGWCC 2 cut(s) 535, 1724
BaeGI GKGCMC 1 cut(s) 729
BaeI ACNNNNGTAYC 2 cut(s) 1360, 1393
BanI GGYRCC 2 cut(s) 724, 1180
BanII GRGCYC 1 cut(s) 668
BbrPI CACGTG 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 1063
BbvI GCAGC 8 cut(s) 38, 67, 187, 384, 506, 1264, 1534, 1689
BccI CCATC 1 cut(s) 1654
BceAI ACGGC 2 cut(s) 120, 155
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciVI GTATCC 1 cut(s) 184
BcnI CCSGG 1 cut(s) 1518
BcoDI GTCTC 7 cut(s) 24, 143, 228, 1100, 1166, 1214, 1419
BfaI CTAG 1 cut(s) 1707
BfmI CTRYAG 1 cut(s) 1689
BfuI GTATCC 1 cut(s) 184
Bme1390I CCNGG 1 cut(s) 1518
Bme18I GGWCC 2 cut(s) 535, 1724
BmgBI CACGTC 1 cut(s) 1124
BmgT120I GGNCC 3 cut(s) 535, 1246, 1724
BmiI GGNNCC 3 cut(s) 726, 1182, 1248
BmrFI CCNGG 1 cut(s) 1518
BmsI GCATC 6 cut(s) 394, 472, 950, 1058, 1154, 1469
BpiI GAAGAC 1 cut(s) 1063
BpmI CTGGAG 1 cut(s) 1065
Bpu10I CCTNAGC 1 cut(s) 1581
Bpu14I TTCGAA 3 cut(s) 419, 1471, 1761
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 1518
BsaAI YACGTR 1 cut(s) 151
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 3 cut(s) 273, 686, 1635
BsaWI WCCGGW 3 cut(s) 176, 226, 1148
Bsc4I CCNNNNNNNGG 4 cut(s) 274, 647, 1048, 1523
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 2 cut(s) 561, 596
Bse3DI GCAATG 2 cut(s) 382, 912
BseDI CCNNGG 3 cut(s) 273, 686, 1635
BseGI GGATG 3 cut(s) 965, 1145, 1256
BseLI CCNNNNNNNGG 4 cut(s) 274, 647, 1048, 1523
BseMI GCAATG 2 cut(s) 382, 912
BseMII CTCAG 4 cut(s) 318, 803, 1430, 1647
BseNI ACTGG 2 cut(s) 561, 596
BseRI GAGGAG 3 cut(s) 203, 262, 1253
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 8 cut(s) 38, 67, 187, 384, 506, 1264, 1534, 1689
BshFI GGCC 1 cut(s) 1247
BshNI GGYRCC 2 cut(s) 724, 1180
BsiSI CCGG 6 cut(s) 8, 177, 227, 938, 1149, 1517
BslFI GGGAC 1 cut(s) 1380
BslI CCNNNNNNNGG 4 cut(s) 274, 647, 1048, 1523
BsmAI GTCTC 7 cut(s) 24, 143, 228, 1100, 1166, 1214, 1419
BsmBI CGTCTC 3 cut(s) 24, 143, 228
BsmFI GGGAC 1 cut(s) 1380
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 1 cut(s) 1247
Bso31I GGTCTC 1 cut(s) 1100
Bsp119I TTCGAA 3 cut(s) 419, 1471, 1761
Bsp1286I GDGCHC 2 cut(s) 668, 729
Bsp143I GATC 1 cut(s) 1477
BspANI GGCC 1 cut(s) 1247
BspCNI CTCAG 4 cut(s) 317, 804, 1429, 1648
BspLI GGNNCC 3 cut(s) 726, 1182, 1248
BspT104I TTCGAA 3 cut(s) 419, 1471, 1761
BspT107I GGYRCC 2 cut(s) 724, 1180
BspTNI GGTCTC 1 cut(s) 1100
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 2 cut(s) 382, 912
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 2 cut(s) 561, 596
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 3 cut(s) 273, 686, 1635
BssMI GATC 1 cut(s) 1477
BssNI GRCGYC 1 cut(s) 96
Bst4CI ACNGT 3 cut(s) 44, 1413, 1607
Bst6I CTCTTC 4 cut(s) 20, 1164, 1270, 1758
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 151
BstBI TTCGAA 3 cut(s) 419, 1471, 1761
BstC8I GCNNGC 6 cut(s) 185, 747, 948, 1036, 1073, 1552
BstDEI CTNAG 7 cut(s) 304, 812, 1103, 1404, 1416, 1581, 1656
BstEII GGTNACC 1 cut(s) 44
BstF5I GGATG 3 cut(s) 965, 1145, 1256
BstHHI GCGC 2 cut(s) 170, 1282
BstKTI GATC 1 cut(s) 1480
BstMAI GTCTC 7 cut(s) 24, 143, 228, 1100, 1166, 1214, 1419
BstMBI GATC 1 cut(s) 1477
BstMWI GCNNNNNNNGC 4 cut(s) 141, 491, 943, 1560
BstNSI RCATGY 2 cut(s) 749, 1034
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 1516
BstSFI CTRYAG 1 cut(s) 1689
BstSLI GKGCMC 1 cut(s) 729
BstV1I GCAGC 8 cut(s) 38, 67, 187, 384, 506, 1264, 1534, 1689
BstV2I GAAGAC 1 cut(s) 1063
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 1 cut(s) 1247
BtgZI GCGATG 2 cut(s) 26, 1314
BtrI CACGTC 1 cut(s) 1124
BtsCI GGATG 3 cut(s) 965, 1145, 1256
BtsIMutI CAGTG 1 cut(s) 1612
Cac8I GCNNGC 6 cut(s) 185, 747, 948, 1036, 1073, 1552
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 2 cut(s) 170, 1282
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 535, 1246, 1724
CseI GACGC 4 cut(s) 41, 43, 85, 251
Csp6I GTAC 3 cut(s) 278, 342, 1052
CspCI CAANNNNNGTGG 2 cut(s) 1588, 1623
CviAII CATG 8 cut(s) 676, 746, 1031, 1061, 1099, 1339, 1441, 1446
CviQI GTAC 3 cut(s) 278, 342, 1052
DdeI CTNAG 7 cut(s) 304, 812, 1103, 1404, 1416, 1581, 1656
DpnI GATC 1 cut(s) 1479
DpnII GATC 1 cut(s) 1477
DraIII CACNNNGTG 1 cut(s) 151
Eam1104I CTCTTC 4 cut(s) 20, 1164, 1270, 1758
EarI CTCTTC 4 cut(s) 20, 1164, 1270, 1758
EciI GGCGGA 2 cut(s) 122, 131
Eco24I GRGCYC 1 cut(s) 668
Eco31I GGTCTC 1 cut(s) 1100
Eco47I GGWCC 2 cut(s) 535, 1724
Eco72I CACGTG 1 cut(s) 151
Eco91I GGTNACC 1 cut(s) 44
EcoO65I GGTNACC 1 cut(s) 44
EcoRI GAATTC 2 cut(s) 349, 1757
EcoT38I GRGCYC 1 cut(s) 668
Esp3I CGTCTC 3 cut(s) 24, 143, 228
FaeI CATG 8 cut(s) 679, 749, 1034, 1064, 1102, 1342, 1444, 1449
FaqI GGGAC 1 cut(s) 1380
FatI CATG 8 cut(s) 675, 745, 1030, 1060, 1098, 1338, 1440, 1445
FauI CCCGC 3 cut(s) 165, 1080, 1552
FblI GTMKAC 1 cut(s) 588
FokI GGATG 3 cut(s) 972, 1132, 1243
FriOI GRGCYC 1 cut(s) 668
FspBI CTAG 1 cut(s) 1707
GlaI GCGC 2 cut(s) 169, 1281
GsuI CTGGAG 1 cut(s) 1065
HaeIII GGCC 1 cut(s) 1247
HapII CCGG 6 cut(s) 8, 177, 227, 938, 1149, 1517
HgaI GACGC 4 cut(s) 41, 43, 85, 251
HhaI GCGC 2 cut(s) 170, 1282
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 8 cut(s) 679, 749, 1034, 1064, 1102, 1342, 1444, 1449
Hin6I GCGC 2 cut(s) 168, 1280
HinP1I GCGC 2 cut(s) 168, 1280
HinfI GANTC 8 cut(s) 295, 421, 557, 715, 794, 1222, 1468, 1488
HpaII CCGG 6 cut(s) 8, 177, 227, 938, 1149, 1517
HphI GGTGA 7 cut(s) 38, 67, 239, 257, 290, 1163, 1178
Hpy166II GTNNAC 2 cut(s) 589, 1132
Hpy188III TCNNGA 5 cut(s) 329, 505, 957, 1082, 1269
Hpy8I GTNNAC 2 cut(s) 589, 1132
Hpy99I CGWCG 2 cut(s) 71, 101
HpyAV CCTTC 5 cut(s) 214, 547, 1270, 1356, 1515
HpyCH4III ACNGT 3 cut(s) 44, 1413, 1607
HpyCH4IV ACGT 4 cut(s) 69, 150, 585, 1123
HpyCH4V TGCA 9 cut(s) 235, 372, 395, 494, 701, 922, 1034, 1460, 1554
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 491, 943, 1560
HpyF3I CTNAG 7 cut(s) 304, 812, 1103, 1404, 1416, 1581, 1656
HpySE526I ACGT 4 cut(s) 69, 150, 585, 1123
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 8 cut(s) 679, 749, 1034, 1064, 1102, 1342, 1444, 1449
HspAI GCGC 2 cut(s) 168, 1280
Kzo9I GATC 1 cut(s) 1477
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 8 cut(s) 38, 67, 187, 384, 506, 1264, 1534, 1689
LweI GCATC 6 cut(s) 394, 472, 950, 1058, 1154, 1469
MaeI CTAG 1 cut(s) 1707
MaeII ACGT 4 cut(s) 69, 150, 585, 1123
MaeIII GTNAC 8 cut(s) 3, 44, 263, 425, 563, 1318, 1376, 1630
MalI GATC 1 cut(s) 1479
MbiI CCGCTC 1 cut(s) 312
MboI GATC 1 cut(s) 1477
MhlI GDGCHC 2 cut(s) 668, 729
MluCI AATT 9 cut(s) 349, 764, 799, 826, 842, 1454, 1482, 1674, 1757
MlyI GAGTC 1 cut(s) 1216
MmeI TCCRAC 3 cut(s) 665, 771, 1770
MroXI GAANNNNTTC 1 cut(s) 1717
MseI TTAA 5 cut(s) 63, 621, 735, 1229, 1740
MslI CAYNNNNRTG 1 cut(s) 1137
MspA1I CMGCKG 3 cut(s) 51, 80, 1547
MspI CCGG 6 cut(s) 8, 177, 227, 938, 1149, 1517
MspR9I CCNGG 1 cut(s) 1518
Mva1269I GAATGC 1 cut(s) 514
MwoI GCNNNNNNNGC 4 cut(s) 141, 491, 943, 1560
NciI CCSGG 1 cut(s) 1518
NdeII GATC 1 cut(s) 1477
NlaIII CATG 8 cut(s) 679, 749, 1034, 1064, 1102, 1342, 1444, 1449
NlaIV GGNNCC 3 cut(s) 726, 1182, 1248
NmuCI GTSAC 5 cut(s) 3, 44, 263, 1318, 1376
NspI RCATGY 2 cut(s) 749, 1034
NspV TTCGAA 3 cut(s) 419, 1471, 1761
PaeI GCATGC 1 cut(s) 749
PctI GAATGC 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 1717
PfeI GAWTC 7 cut(s) 295, 421, 557, 715, 794, 1468, 1488
PleI GAGTC 1 cut(s) 1216
PmaCI CACGTG 1 cut(s) 151
PmlI CACGTG 1 cut(s) 151
PpsI GAGTC 1 cut(s) 1216
Ppu21I YACGTR 1 cut(s) 151
PspCI CACGTG 1 cut(s) 151
PspEI GGTNACC 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 726, 1182, 1248
PspPI GGNCC 3 cut(s) 535, 1246, 1724
PstNI CAGNNNCTG 1 cut(s) 485
RsaI GTAC 3 cut(s) 279, 343, 1053
RsaNI GTAC 3 cut(s) 278, 342, 1052
RseI CAYNNNNRTG 1 cut(s) 1137
SaqAI TTAA 5 cut(s) 63, 621, 735, 1229, 1740
Sau3AI GATC 1 cut(s) 1477
Sau96I GGNCC 3 cut(s) 535, 1246, 1724
SchI GAGTC 1 cut(s) 1216
ScrFI CCNGG 1 cut(s) 1518
SduI GDGCHC 2 cut(s) 668, 729
SfaNI GCATC 6 cut(s) 394, 472, 950, 1058, 1154, 1469
SfcI CTRYAG 1 cut(s) 1689
SfuI TTCGAA 3 cut(s) 419, 1471, 1761
SgrAI CRCCGGYG 1 cut(s) 7
SinI GGWCC 2 cut(s) 535, 1724
SmiMI CAYNNNNRTG 1 cut(s) 1137
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SphI GCATGC 1 cut(s) 749
Sse9I AATT 9 cut(s) 349, 764, 799, 826, 842, 1454, 1482, 1674, 1757
SspMI CTAG 1 cut(s) 1707
StyD4I CCNGG 1 cut(s) 1516
TaaI ACNGT 3 cut(s) 44, 1413, 1607
TaiI ACGT 4 cut(s) 72, 153, 588, 1126
TaqI TCGA 7 cut(s) 328, 419, 1270, 1360, 1463, 1471, 1761
TasI AATT 9 cut(s) 349, 764, 799, 826, 842, 1454, 1482, 1674, 1757
TatI WGTACW 1 cut(s) 341
TauI GCSGC 3 cut(s) 86, 96, 383
TfiI GAWTC 7 cut(s) 295, 421, 557, 715, 794, 1468, 1488
Tru1I TTAA 5 cut(s) 63, 621, 735, 1229, 1740
Tru9I TTAA 5 cut(s) 63, 621, 735, 1229, 1740
TscAI CASTG 1 cut(s) 1612
TseFI GTSAC 5 cut(s) 3, 44, 263, 1318, 1376
TseI GCWGC 8 cut(s) 51, 80, 200, 372, 494, 1277, 1547, 1702
Tsp45I GTSAC 5 cut(s) 3, 44, 263, 1318, 1376
TspDTI ATGAA 7 cut(s) 29, 588, 901, 1245, 1317, 1414, 1610
TspGWI ACGGA 2 cut(s) 210, 1382
TspRI CASTG 1 cut(s) 1612
VpaK11BI GGWCC 2 cut(s) 535, 1724
XapI RAATTY 6 cut(s) 349, 764, 799, 842, 1454, 1757
XceI RCATGY 2 cut(s) 749, 1034
XmiI GTMKAC 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 1717
XspI CTAG 1 cut(s) 1707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.